Duration of blood plasma cortisol suppression after a low-dose dexamethasone suppression test in dogs
Abstract The aim of this study was to determine the duration of the suppressive effect on the hypothalamus–pituitary–adrenocortical axis as measured by plasma cortisol concentration after injection of a low dosage of dexamethasone (0.01 mg/kg BW) used within the low-dose dexamethasone suppression test (LDDST). A LDDST was performed on ten clinically healthy dogs with unremarkable haematological and plasma biochemical profiles, abdominal X-ray, abdominal ultrasonography and fine needle aspiration cytology of the liver. Two of them were excluded from the analysis. Blood samples were collected at bas...
Source: Comparative Clinical Pathology - November 27, 2015 Category: Pathology Source Type: research

Expression of metallothionein protein relating to proliferative cell index in malignant feline mammary tumors using high throughput tissue microarray technique
Abstract The present study aimed to examine the relationship between proliferation markers by detecting Ki-67 and proliferative cell nuclear antigen (PCNA) indices, and metallothionein expression, in different histological groups and nuclear grades of malignant feline mammary tumors by using immunohistochemistry and tissue microarray technique. Seventy feline mammary carcinoma (FMC) biopsy specimens were collected to perform tissue microarray (TMA) slide. The immunohistochemical staining was performed on the TMA slides against pancytokeratin (CK19), PCNA, Ki-67 clone MIB, and metallothionein 1 (MT-1) antibodies. T...
Source: Comparative Clinical Pathology - November 27, 2015 Category: Pathology Source Type: research

Prognostic value and clinical significance of TCR rearrangements for MRD monitoring in ALL patients
In this study, we have discussed T cell receptor (TCR) gene rearrangements in ALL. As leukemia cell proliferation originates from a unique clone, clone-specific rearrangement can be very helpful in diagnosis and detection of remaining malignant cells from among normal cells for minimal residual disease. On the other hand, each of the TCR genes is associated with translocation in ALL types, and the result of gene rearrangements can be used as prognosis markers. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 23, 2015 Category: Pathology Source Type: research

Renal structure and its concentrating ability in the Persian squirrel ( Sciurus anomalus )
Abstract The present study was conducted to investigate the morphological characteristics of the kidney and its urine concentrating ability in the Persian squirrel. The kidneys of five male Persian squirrel which suffered from diseases unrelated to the urogenital system were used for morphometric measurements and biochemical analysis of blood and urine under their owner’s permission. There was no statistical significance found between the linear measurements (length, width, and thickness) between the right and left kidneys (P > 0.05). Also, no statistically significant difference existed bet...
Source: Comparative Clinical Pathology - November 23, 2015 Category: Pathology Source Type: research

The case report on cutaneous myxoma or invasive myxomatosis in Blackfin sea catfish ( Arius jella Day, 1877)
Abstract The presence of cutaneous myxoma is reported in a Blackfin sea catfish (Arius jella) from Vellar estuary, Parangipettai, southeast coast of India. The gross morphology revealed fleshy with gelatinous distended tumourous growths on both sides of the fish attached to the caudal peduncle region. Tumour overlying the epidermis was ulcerated, hyperemic and hyperchromatic. Histologically, the tumourous growth was characterized by bland spindloid cells embedded in an abundant myxoid extracellular matrix. The tumour masses were present within the dermis and covered with a thin attenuated layer of epidermis. Neopl...
Source: Comparative Clinical Pathology - November 19, 2015 Category: Pathology Source Type: research

Ectopic cross ureter with uterus didelphys in a lamb
Abstract The development and incorporation of the ureteric bud into the developing urinary bladder is a complex process, which is simultaneously influenced by events occurring in the development of other systems in the perineum. Knowledge of the embryology of the ureteric bud and associated Wolffian duct is the key to understand the development of the entire genitourinary system. In this article, we have reported and discussed a 1-day dead lamb with congenital ectopic ureter, open urachal sinus and uterus didelphys. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 17, 2015 Category: Pathology Source Type: research

Hepatocellular carcinoma in a cow: detection of tumor-infiltrating T lymphocytes implicated in anti-tumor immune response and partial spontaneous tumor regression
This report describes the morphological finding in a 12-year-old Holstein-Friesian dairy cow with spontaneous hepatocellular carcinoma. The cow that showed anorexia, emaciation, depression, and sluggish movement was culled and exhibited multiple tumor nodules (
Source: Comparative Clinical Pathology - November 17, 2015 Category: Pathology Source Type: research

Survivin and SIRT1: can be two prognostic factors in chronic myeloid leukemia?
In this study, we measured the expression of SVV and SIRT1 in 18 patients with typical Ph-positive chronic myeloid leukemia in chronic phases of the disease as well as in 18 matched healthy donors. SVV, SIRT1, and BCR/ABL expression were determined by quantitative-real-time polymerase chain reaction (Q-RT-PCR). HPRT was used as a reference gene to calculate SVV/HPRT and SIRT1/HPRT ratio, and ABL was used as an internal control for BCR/ABL expression. SVV was between 0.00 and 2.39-fold (median 0.204) and SIRT1 was between 0.00 and 33.32-fold (median 0.186). In our study, both SVV and SIRT1 were lower in patients than in the...
Source: Comparative Clinical Pathology - November 16, 2015 Category: Pathology Source Type: research

Culture-dependent assessment of bacterial diversity from human milk with lactational mastitis
The objective of this study was to explore culturable bacterial diversity present in the breast milk of mastitis-suffering women by 16S rDNA sequencing and evaluate its antibiotic susceptibility. The culturable bacteria present in the breast milk of 32 women with lactational mastitis were studied. Thirty-seven bacterial isolates comprised of distinct colony characteristics were checked for antibiotic susceptibility and identified by 16S rDNA sequencing. Sequencing of 16S rDNA and BLAST analysis revealed the presence of 17 genera and 30 different species indicating highly diverse community in mastitis milk. The Proteobacter...
Source: Comparative Clinical Pathology - November 16, 2015 Category: Pathology Source Type: research

Evaluation of clinical, hematological, and biochemical changes following autologous blood transfusion in goats
Abstract Blood transfusion is an important remedy to manage animals with severe anemia. The risk of pathogen transmission can be reduced using autologous blood transfusion. Due to the lack of studies related to the use of autologous blood transfusions in goats, this study was conducted to evaluate the clinical, hematological, and biochemical changes following blood collection and reinfusion in goats. Fifteen milliliters per kilogram of whole blood was collected and reinfused after 7 days. The heart rate and respiratory rate were increased following blood collection and decreased to normal values within 48 and...
Source: Comparative Clinical Pathology - November 16, 2015 Category: Pathology Source Type: research

Histopathological evaluation of the healing effects of human amniotic membrane transplantation in third-degree burn wound injuries
Abstract Skin burn injuries result in loss of its protective function as a barrier and leading to a high risk of infection. Therefore, effective treatments and healing of burn injuries are very important to prevent complications. Amniotic membrane (AM) as a biological dressing inhibits the loss of vital fluids, water, and protein. The aim of this study was to compare the healing effects of AM and silver sulfadiazine (SSD) ointment in third-degree burn injuries in experimental rat model. Fifty-four male Sprague–Dawley rats were divided randomly into three equal groups. After induction of third-degree burn, tr...
Source: Comparative Clinical Pathology - November 11, 2015 Category: Pathology Source Type: research

Orexin-A improves ischaemia/reperfusion-induced acute renal failure
This study investigated the effect of orexin-A on the renal dysfunction and histological damage induced by ischaemia/reperfusion at an early stage. Pentobarbital anaesthetised rats were prepared for measuring renal functional variables. Ischaemia was induced by bilateral renal artery clamping for 30 min followed by a 1-h reperfusion period. In orexin-treated rats, it was infused (i.v.) at 500 pmol kg−1 min−1 from starting pre-ischaemic (pretreatment) clearance period. The left kidney was examined using light microscopy. The renal ischaemic challenge resulted in major histological damages, wh...
Source: Comparative Clinical Pathology - November 10, 2015 Category: Pathology Source Type: research

The effect of fetal rat brain extract on morphology of bone marrow-derived mesenchymal stem cells
Abstract Nowadays, regenerative medicine is going to find its true position among different kinds of treatment measures. In this regard, cell therapy brings hopes for the treatment of various diseases. As it is obvious, the role of nervous system for the appropriate function of other organs is not negligible. Unfortunately, damage to nerve cells is irreversible. Substitution of malfunctioned neural cells with the normal one is a noteworthy choice. The aim of the present study was to determine the effect of fetal rat brain extract on morphology of bone marrow-derived mesenchymal stem cells (BM-MSCs). Hence, pooled ...
Source: Comparative Clinical Pathology - October 5, 2015 Category: Pathology Source Type: research

Blood smear demonstrations of Anaplasma -infected sheep in a flock
This study is important because it was done in a naturally Anaplasma-infected flock, emphasized on valuable changes in the infected blood films, and discussed the importance of preventive measures in an area. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - October 1, 2015 Category: Pathology Source Type: research

Incidence of dermatophytes isolated from dogs and cats in the city of Baku, Azerbaijan
Abstract Dermatophytes are filamentous fungi, which are able to use keratin as a source of carbon. Some of these organisms develop in skin and hair and cause cutaneous lesions. This zoonotic disease is called dermatophytosis or ringworm. The study was aimed to assess the frequency of dermatophyte infection of dog’s and cat’s skin around the city of Baku. During a period of 1 year (2014–2015), 116 dogs and 77 cats were included in this study. The mycological analyses were conducted by direct microscopy and by fungal culture on chloramphenicol Sabouraud agar (CSA) at the Department of Microbio...
Source: Comparative Clinical Pathology - September 30, 2015 Category: Pathology Source Type: research

Follicular adenoma associated with spindle cell proliferation, papillary adenoma and colloid goitre in a dromedary camel
Abstract In humans, spindle cell lesions associated with thyroid adenoma or carcinoma are rare. A cross section of the thyroid of a dromedary camel showed follicular cysts, and microscopically, both thyroid lobes showed colloid goitre. The left lobe showed spindle cell proliferation associated with follicular adenoma without evidence of capsular or vascular invasion. The spindle cell lesion had cells featuring ground glass or pseudoinclusion nuclei and showed extensive haemorrhage, hyalinization and thrombosis. The right thyroid lobe showed papillary adenoma. Immunohistochemistry showed negative expression for mal...
Source: Comparative Clinical Pathology - September 26, 2015 Category: Pathology Source Type: research

Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves ( Bubalus bubalis )
This study was designed to present detailed detection and histopathological changes of the reproductive system and mammary glands of buffaloes experimentally infected with P. multocida B:2. Briefly, nine healthy buffalo calves aged 8-month-old were divided into three groups of three calves each. Calves from groups 1 and 2 were inoculated subcutaneously and orally with 10 mL of 1 × 1012 cfu/mL of P. multocida B:2, respectively, while calves of group 3 were inoculated orally with 10 mL of phosphate buffer saline as a sham control. All the calves in group 1 had to be euthanized after 12 ...
Source: Comparative Clinical Pathology - September 25, 2015 Category: Pathology Source Type: research

Effect of piracetam, vincamine, vinpocetine, and donepezil on oxidative stress and neurodegeneration induced by aluminum chloride in rats
In this study, we investigated the effects of the memory-enhancing drugs piracetam, vincamine, and vinpocetine or the cholinesterase inhibitor donepezil on the development of oxidative stress, inflammation, and brain damage induced in rat brain by aluminum chloride (AlCl3). Saline (control), piracetam (100 or 300 mg/kg), vincamine (10 or 20 mg/kg), vinpocetine (10 or 20 mg/kg), piracetam 100 mg/kg plus vincamine 10 mg/kg, piracetam 100 mg/kg plus vinpocetine 10 mg/kg, or donepezil 5 mg/kg were administered once daily intraperitoneally for 45 days along with AlCl3 (10 mg/kg, intr...
Source: Comparative Clinical Pathology - September 22, 2015 Category: Pathology Source Type: research

Evaluation of the effects of methanol leaf extract of Palisota hirsuta on the biochemical and hematological parameters of rats
Abstract The effects of 28-day oral administration of the methanol leaf extract of Palisota hirsuta (MLEPH) on biochemical, hematological parameters, and relative organ weights (ROWs) in albino rats were investigated. There were no significant (p > 0.05) variations in the ROW of the heart, kidney, spleen, and liver on days 14 and 28 in all the rats given the different doses of MLEPH. The hematology showed that there were no significant (p > 0.05) alterations of the packed cell volume (PCV), hemoglobin concentration, RBC count, total white blood cell (tWBC), and differential WBC co...
Source: Comparative Clinical Pathology - September 22, 2015 Category: Pathology Source Type: research

Evaluation of the osteogenic potentials of the platelet-rich plasma and autologous cancellous bone grafts on ulna ostectomies of Nigerian local dogs
This study established that for optimal osteogenesis, concurrent application of PRP and CBG should be advocated. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - September 16, 2015 Category: Pathology Source Type: research

CD4+ and CD8+ T cells in the peripheral blood of dogs affected with generalised demodicosis
Abstract Canine generalised demodicosis is recognised as a severe skin disease of dogs, and studies on CD4+ and CD8+ T cells in this disease condition have been limited. The aim of the present study was to record the CD4+ and CD8+ T cells in the peripheral blood of dogs affected with generalised demodicosis. Whole blood was collected from dogs with generalised demodicosis for estimation of CD4+ and CD8+ T cells by flow cytometry. The demodicosis-affected dogs had lower number of CD4+ T cells, increased CD8+ T cells, and reduced CD4+/CD8+ ratio compared to healthy controls. Present abnormalities in the study are re...
Source: Comparative Clinical Pathology - September 15, 2015 Category: Pathology Source Type: research

Liver function, paraclinical tests, and mortality risk factors in pediatric liver transplant candidates
Abstract Liver transplantation has become a well-recognized transplant modality for children with end-stage liver disease. The first indicator to evaluate its efficiency is mortality rate. The aim of this study is to evaluate the complications and mortality of liver disease in children waiting for transplantation and understanding the risk factors that predict liver transplant waiting list death. This is a single-center prospective cohort study of all patient ≤18 years of age with chronic liver disease listed for liver transplantation. We analyzed medical records of 130 children for mortality risk factor. ...
Source: Comparative Clinical Pathology - September 11, 2015 Category: Pathology Source Type: research

Ethanolic extract of Salvia officinalis alter vascular endothelial growth factor gene expression and its receptors in the mouse endometrium at preimplantation
Abstract Salvia officinalis contains steroid and isoflavonoid coumestrol, daidzein, and genistein which are considered as phytoestrogens. Isoflavones are alternatives to hormone replacement treatment. No studies have been conducted on the effect of S. officinalis extraction on endometrial angiogenesis yet. The adult female mice in the test group received an ethanolic extract of the S. officinalis (100 mg/kg) once a day for 14 days then pseudopregnancy was induced. Uterine horns were removed for molecular and histological assessment in each group. Motic image software was used to assess the...
Source: Comparative Clinical Pathology - September 7, 2015 Category: Pathology Source Type: research

Changes in haematological parameters and oxidative stress response of goats subjected to road transport stress in a hot humid tropical environment
This study investigated the haematological parameters and oxidative stress response of goats subjected to 7 h of road transportation in the hot, humid tropical environment and the possible recovery period. Thirty-five healthy Boer goats, aged 2–3, weighing 20–25 kg, were divided into two groups, designated A and B, of 30 and 5 animals, respectively. Group A was transported for 7 h, and blood samples were collected before, 3.5 h on transit, after transport, and on days 3, 7, 16 and 26 post-transport, while five goats served as control. Plasma and haemolysate were prepared and used to assay ma...
Source: Comparative Clinical Pathology - September 7, 2015 Category: Pathology Source Type: research

Transmission electron microscopy of the retina following thyroidectomy in rabbit
In this study, 10 male rabbits of New Zealand strips were divided into two groups of five animals each (control group and thyroidectomy group). Before surgery, the rabbits were maintained in 12-h light and 12-h dark. The animals were anesthetized with intraperitoneal injection of 10 mg/kg xylazine and 6 mg/kg ketamine, and bilateral thyroidectomy was performed. One and 2 weeks after surgery, the rabbits were sacrificed by humane methods. Then, retinas of the killed rabbits were isolated in the laboratory and examined by electron microscopy for evaluating cells and changes in inner segment, outer segment, out...
Source: Comparative Clinical Pathology - September 1, 2015 Category: Pathology Source Type: research

Effects of dietary supplementation of Ferulago angulata (Schlecht.) Boiss powder on growth performance, carcass characteristics, and gut microflora and pH in broiler chicks
This study was conducted to examine the effects of powder of aerial parts of Ferulago angulata (FA) on performance, carcass characteristics, and intestinal development and microflora of broilers. A total of 126 1-day-old straight run broiler chicks (Ross 308) were assigned randomly into three dietary groups each with three replicates of 14 chicks. The dietary groups included a corn-soybean meal basal diet (control) and basal diet supplemented with 0.1 or 0.2 % FA powder. The experiment lasted for 42 days. In the overall period, chicks fed diets supplemented with FA gained more (P 
Source: Comparative Clinical Pathology - September 1, 2015 Category: Pathology Source Type: research

Assessment of the hepatic and renal effects of sub-chronic administration of sub-lethal doses of amitraz/xylene in albino Wistar rats
Abstract The toxicological effects of increasing doses of amitraz on the liver and kidney of animals treated with it need to be assessed as a recent trend in development of resistance by pests may lead to increasing the quantity of amitraz required to control them. Forty adult male rats 170 ± 10 g assigned using simple random sampling method into four groups were used for the study. Groups A, B, and C were treated daily with 10.0, 2.0 and 0.4 mg/kg body weight, bw of amitraz respectively while group D (control group) was treated with 10 ml/kg bw water. The treatment was done dai...
Source: Comparative Clinical Pathology - August 20, 2015 Category: Pathology Source Type: research

The effect of human amniotic membrane as a covering layer on propylene mesh in decrease of adhesion after laparotomy in the rabbit
This study was conducted in order to determine surgical and histological outcomes of polypropylene mesh coated with human amniotic membrane (HAM) in comparison with polypropylene mesh on its own. Ten rabbits were divided into two equal groups, group I and II. A 5-cm incision was made along the abdomen of each rabbit. In group I, a 2 × 3 cm layer of polypropylene mesh was placed under the wall of the abdomen adjacent to the viscera. In group II, the procedure was identical except that the mesh was covered by a 4 × 6 cm layer of HAM too. After 3 weeks, all animals were s...
Source: Comparative Clinical Pathology - August 19, 2015 Category: Pathology Source Type: research

Coagulation parameters of captive black-tailed prairie dogs ( Cynomys ludovicianus )
This study was conducted in order to determine coagulation parameters in captive black-tailed prairie dogs. Sixteen black-tailed prairie dogs (10 males, 6 females) were presented for annual health evaluations. A blood sample was collected from the jugular vein of each prairie dog while under general isoflurane anesthesia, and prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen and fibrin degradation products (FDP) were evaluated using an automated hemostasis analyzer. Results of these tests showed a median PT of 8.4 s and a median APTT of 23.8 s. Median fibrinogen concentration was 343...
Source: Comparative Clinical Pathology - August 16, 2015 Category: Pathology Source Type: research

Improving lithium carbonate therapeutics by pegylated liposomal technology: an in vivo study
In this study, pegylated liposomal lithium carbonate nanoparticles were prepared by the reverse phase evaporation method to improve the drug’s therapeutic characteristics as well as lessening its side effects. In order to synthesize pegylated liposomal lithium carbonate, phosphatidylcholine, polyethylene glycol 3350 (PEG3350), cholesterol, and lithium carbonate were mixed. The characterization of synthesized nanoparticles was determined by Zetasizer. Encapsulation and drug loading efficiency and release pattern studies were determined through spectrophotometry method. In addition, serum lithium and creatinine levels ...
Source: Comparative Clinical Pathology - August 14, 2015 Category: Pathology Source Type: research

Confocal microscopic analysis of erythrocytes and erythroplastids from Major Mitchell’s cockatoos ( Lophochroa leadbeateri )
Abstract The majority of avian erythrocytes retain their nucleus throughout the circulatory lifespan of the cell; however, a small proportion of avian erythrocytes are enucleated to form erythroplastids. Previously, we have reported a Major Mitchell’s cockatoo that had a much greater proportion of erythroplastids than typically observed in avian blood. To further classify the erythrocytes of this bird, we employed fluorescent laser scanning confocal microscopy. Fluorescence of the cytoplasm of erythroid cells, including erythroplastids, facilitated assessment including z-stack analysis. Erythroplastids had a...
Source: Comparative Clinical Pathology - August 13, 2015 Category: Pathology Source Type: research

Intra-cardiac autonomic neuropathy in Holstein-Friesian dairy cows affected with bovine dilated cardiomyopathy
Abstract Three Holstein-Friesian dairy cows, aged 5 1/3 to 12 years, showed a history of heart failure and were euthanized on separate days. Commonly observed postmortem findings included edema in many sites of the body, dilatation and hypertrophy of the right cardiac ventricle, hepatic congestion, and swollen pale kidneys. Histopathologically, myocardial lesions were comprised of a variety of degenerative changes in cardiomyocytes and interstitial fibrosis, consistent with those described previously in cases of bovine dilated cardiomyopathy in Holstein-Friesian cattle. Additionally, in one of these three cow...
Source: Comparative Clinical Pathology - August 8, 2015 Category: Pathology Source Type: research

Rhodococcus equi : are phenotypic tests the best method for accurate identification?
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - August 8, 2015 Category: Pathology Source Type: research

Bacillus cereus infection-associated pneumonia in an African grey parrot ( Psittacus erithacus erithacus )
Abstract Bacillus cereus infection-associated suppurative pneumonia was recorded in an adult African grey parrot (Psittacus erithacus erithacus). Grossly, the diaphragmatic lung lobes showed presence of multiple, irregular, creamy colored areas over the surface. The adjacent parenchyma appeared congested and pneumonic. Histopathologically, multifocal areas of abscesses characterized by presence of necrotic cell shreds, fibrin, and collection of heterophils with presence of bacterial aggregates were observed in the lungs. Taylor’s staining revealed presence of multiple aggregates of Gram-positi...
Source: Comparative Clinical Pathology - August 7, 2015 Category: Pathology Source Type: research

A histology-based study in apparently healthy wild freshwater stingrays Potamotrygon motoro
Abstract Histopathological studies are important as a reference in pathological process. There are a few of these studies in elasmobranchs. The aim of this study was to describe the histology of kidney, spleen, liver, and gills of wild apparently healthy Potamotrygon motoro. However, we observed some pathological findings in these animals. Nineteen P. motoro were collected from the Parana River, Brazil. Histology of kidney, spleen, liver, and gills were performed according to usual procedures. Briefly, the organs were immersed in 10 % formaldehyde solution for 48 h and routinely processed, embedded in pa...
Source: Comparative Clinical Pathology - August 5, 2015 Category: Pathology Source Type: research

Intravascular lymphoma preceded by urinary retention and diagnosed by random skin biopsy
Abstract A 53-year-old man was hospitalized for the scrutinization of urinary disturbance. Although vertebral X-ray did not show bone destruction, magnetic resonance imaging (MRI) revealed multiple lesions in thoracic and lumbar vertebrae. Bone marrow aspiration and biopsy of the ilium showed no abnormality. Bone marrow biopsy of the fifth lumbar vertebra was performed, and the diagnosis of diffuse large B cell lymphoma was made. As subacute ascending myelopathy of flaccid paraparesis developed, intravascular lymphoma was suspected. Random skin biopsy was then performed from normal-appearing skin. Hematoxylin-eosi...
Source: Comparative Clinical Pathology - July 31, 2015 Category: Pathology Source Type: research

Brain and serum galanin, thyroid hormones, and TSH are not influenced by different photoperiod schedules in broiler chickens
Abstract The purpose of the present study was to evaluate the effect of three different light regimens on serum thyroid hormones (THs), thyroid-stimulating hormone (TSH), and galanin levels, as well as brain galanin concentration, of broiler chickens. To this end, three groups of 1-day-old Cobb 500 broiler chicks were used. Each group consisted of 20 chickens. The chicks were kept in separate light-proof controlled rooms. All birds were reared on continuous light until 1 week of age. Then, the first group was treated under a continuous lighting (CL) program (23L:1D); the second group treated under a non-inter...
Source: Comparative Clinical Pathology - July 31, 2015 Category: Pathology Source Type: research

Effects of reduction of adenosine deaminase activity in the serum of dogs naturally infected by Ehrlichia canis and Hepatozoon canis
Abstract Ehrlichia canis and Hepatozoon canis have been reported infecting a variety of Carnivora species worldwide, including domestic dogs and wild canids. Since both diseases can follow their course while the infected animals remain asymptomatic, assessments of different parameters that may help toward reaching a more accurate diagnosis are welcome. In this regard, the aim of the present study was to assess the activity of adenosine deaminase (ADA) in serum from naturally infected dogs. Forty-nine samples from dogs were used in our study: 15 positive for E. canis (8 asymptomatic and 7 symptomatic...
Source: Comparative Clinical Pathology - July 24, 2015 Category: Pathology Source Type: research

Biological activities, antioxidant properties and phytoconstituents of essential oil from sweet basil ( Ocimum basilicum L.) leaves
This study investigated the biological activities of essential oil from sweet basil leaves via its effect on α-amylase, α-glucosidase and angiotensin-I-converting enzyme (ACE) activities, inhibition of Fe2+ and sodium nitroprusside (SNP)-induced lipid peroxidation in rats’ pancreas and heart homogenates. The phytoconstituents of the oil were analysed using gas chromatography (GC). The essential oil exhibited a dose-dependent inhibition of α-amylase (IC50 = 3.21 mg/mL), α-glucosidase (IC50 = 3.06 mg/mL) and ACE (IC50 = 0.89 mg/mL) activit...
Source: Comparative Clinical Pathology - July 24, 2015 Category: Pathology Source Type: research

Efficacy and safety of a biogeneric recombinant human erythropoietin in the management of chemotherapy-induced anemia: a randomized double-blind active-comparator trial
This study aimed to compare the efficacy and safety of a biogeneric rHuEPO (Epolyrec®) with those of the original brand-name product (Eprex®). Seventy-seven cancer patients with hemoglobin levels 100 ng/dL, and a life expectancy of at least 6 months were recruited into the trial. Included subjects were randomly assigned to either Epolyrec® (n = 37) or Eprex® (n = 40) at a weekly dose of 100 IU/kg (divided in three doses), for a period of 8 weeks. Hemoglobin and hematocrit levels were significantly increased by week 4 in the Epolyrec® group (p &thins...
Source: Comparative Clinical Pathology - July 22, 2015 Category: Pathology Source Type: research

Use of a point-of-care portable blood analyzer for evaluation of blood gas analytes and select physiologic parameters in the American flamingo ( Phoenicopterus ruber )
This study was conducted in order to evaluate blood gas analytes using a portable clinical analyzer in captive American flamingos (Phoenicopterus ruber). Seventeen healthy flamingos were evaluated as part of an overall clinical health assessment. Each bird underwent a complete physical examination, complete blood count, and serum biochemistry testing. Blood samples were collected from the medial metatarsal vein and using the VetScan i-STAT® analyzer, tested for the following parameters: hydrogen ion concentration, oxygen tension, carbon dioxide tension, sodium, potassium, ionized calcium, hematocrit, and various calcul...
Source: Comparative Clinical Pathology - July 22, 2015 Category: Pathology Source Type: research

Ultra-structure of blood cells and molecular characteristics of Haemoproteus sp. in Blyth’s hawk eagle
Abstract Haemoproteus was detected in the blood smear of an adult female Blyth’s hawk eagle which was submitted to the Kasetsart University Raptor Rehabilitation Unit. The morphology and ultra-structure of leukocytes and gamonts of Haemoproteus were described. The gamont’s cytoplasm contained scattered brown to black pigment granules with a mean of 12 granules per parasite. Monocytes played a major role in the elimination of the gamonts. According to the annotated checklist of the avian Haemoproteidae, the Haemoproteus in this eagle was closely related to Haemoproteus buteonis. The phylo...
Source: Comparative Clinical Pathology - July 21, 2015 Category: Pathology Source Type: research

Prevalence of the C282Y and H63D mutations of the HFE hemochromatosis gene in Azerian major β-thalassemia and iron overload
In this study, we have determined the allele frequency of HFE mutations H63D and C282Y in a group of Azerian beta-thalassemia major patients. These two mutations are implicated in hereditary hemochromatosis among Caucasians. In this study, we wanted to outbreak these mutations with the iron status in major beta-thalassemia patients. Sixty Azerian major beta-thalassemias were screening for the C282Y and H63D by digestion of polymerase chain reaction products (PCRP). Serum ferritin level was measured by enzyme-linked immunosorbent assay (ELISA). The allele frequency of H63D mutation was 20 %. C282Y mutation was not pres...
Source: Comparative Clinical Pathology - July 15, 2015 Category: Pathology Source Type: research

Haematologic effects of leukoreduction on canine whole blood post-filtration and post-storage
Abstract Leukoreduction is the removal of leukocytes from a blood component to avoid the adverse reactions resulting from the exposure of leukocytes from a donor to the recipient during transfusion. A leukoreduced blood component has been defined as achieving a concentration of less than 5 × 106 residual donor leukocytes per final blood product while maintaining 85 % of the original red blood cell count. In the present study, we evaluated the efficiency of leukocyte filtration using a BioR 01-plus blood filter (Fresenius Hemocare, Germany) on samples of whole blood in citrate-phosphate-dex...
Source: Comparative Clinical Pathology - July 11, 2015 Category: Pathology Source Type: research

Combination of diminazene aceturate and resveratrol reduces the toxic effects of chemotherapy in treating Trypanosoma evansi infection
Abstract The aim of this study was to evaluate the effects of resveratrol (RSV) in its free or complexed (cyclodextrin) forms in association with diminazene aceturate (DA), as an attempt to reduce the toxic effects of this standard chemotherapy on renal and hepatic samples of mice experimentally infected with T. evansi. In this sense, this experiment was designed in order to test the ability of RSV, in its free or complexed forms, on reducing the damage on lipid membrane and protein oxidation, effects usually observed by using DA. Both forms of RSV were used at 100 mg kg−1, during seven consecutive...
Source: Comparative Clinical Pathology - July 11, 2015 Category: Pathology Source Type: research

The effects of aqueous extracts of Lippia citriodora , Trachyspermum copticum , Dracocepalum polychaetum on repair of ethanol induced gastric ulcer in rats
Abstract To evaluate the effects of Lippia citriodora, Trachyspermum copticum, and Dracocepalum polychaetum on healing of gastric ulcer induced by ethanol, 25 male Sprague–Dawley rats were divided into five groups (five rats in each group). After 24-h starvation, all animals received oral ethanol (1 mL/200 g body weight). Three groups received 2 mL (100 mg/kg/day) of L. citriodora (LC group), D. polychaetum (DP group), and T. copticum (TC group) aqueous extracts; one group (RA) received ranitidine (200 mg/kg/day); and one group (control) received 2 mL distilled water saline by g...
Source: Comparative Clinical Pathology - July 8, 2015 Category: Pathology Source Type: research

Protection by intraperitoneal administration of bone marrow-derived stem cells of lipopolysaccharide-induced brain and liver damage in mice
Abstract Stem cells induce functional improvement in experimental and clinical studies, but its mechanism is still not known. This paper studies the effect of a single systemic injection of bone marrow-derived stem cell (BM-SC) suspension on brain oxidative stress and on pathological changes induced in the brain and liver of mice by lipopolysaccharide (LPS) endotoxin. Moreover, the possible cholinergic modulation of stem cell effects was also studied. Mice were treated with intraperitoneal (i.p.) injection of stem cell suspension or saline with or without subcutaneous atropine (1 mg/kg) at time of LPS (200&nb...
Source: Comparative Clinical Pathology - July 7, 2015 Category: Pathology Source Type: research

Comparative assessment of erythrocyte osmotic fragility in two breeds of dog: Nigerian indigenous breed and the German shepherd
Abstract Haematological analyses are important in early detection of diseases, and breed differences have been suggested (Schalm 1962). This work is a comparative study to establish the influence of breed on erythrocyte osmotic fragility of Nigerian indigenous breed of dog and the German shepherd dog. In the study, erythrocyte values and the erythrocyte osmotic fragility of Nigerian indigenous breed of dog were compared with those of the German shepherd dog. The influence of sex on the erythrocyte values of the German shepherd dog was also investigated. There were no significant differences (P 
Source: Comparative Clinical Pathology - July 1, 2015 Category: Pathology Source Type: research

The antipsychotic drug sulpiride induces insulin intolerance but does not affect body weight in adult male mice
Abstract Research studies have shown that chronic administration of some antipsychotic drugs induces obesity in female but not in male. To explore the mechanisms involved in this effect, we administrated the dopamine antagonist sulpiride (40 mg/kg) or vehicle intraperitoneally to adult male mice during 45 days and then the glucose tolerance and serum levels of insulin, serum, and testicular testosterone and prolactin, and blood estrogen levels were evaluated. Chronic sulpiride administration did not affect body weight. However, prolactin levels and the area under the glucose and insulin curves were signi...
Source: Comparative Clinical Pathology - July 1, 2015 Category: Pathology Source Type: research

Aelurostrongylus abstrusus in a stray cat’s blood stream
Abstract Aelurostrongylus abstrusus (Raillet), the cat lungworm, is a parasitic round worm which lives in the terminal bronchioles and the alveolar ducts inside the lungs of cats as final hosts. Snails or slugs can be intermediate hosts while rodents, frogs, toads, lizards, birds, and snakes can play the role of transporter from the intermediate host into the final host. The ingested third-stage larvae migrate towards the lungs through the blood vessels. In this research, first, the observation of A. abstrusus infection in a stray cat in Iran is described. Later, the larval detection of this worm ...
Source: Comparative Clinical Pathology - July 1, 2015 Category: Pathology Source Type: research