Effect of supplementation of newborn piglets with spray dry blood plasma on weight gain and serum biochemical variables
AbstractThe aim of this study was to evaluate the effect of ​spray dry blood plasma (SDBP) supplementation for newborn piglets on performance, seric biochemical variables, total antioxidant levels, and nitric oxide. Thus, 20 newborn piglets, with lower body wheith (1.179 ± 0.276 kg) were distributed in an experimental design with five randomized blo cks (females were considered block), with two treatments and ten repetitions. The treatment consisted to provide 10 g of SDBP (group T), previously diluted at 1:4 (blood plasma/water) administered for the first hour post-birth of the piglet...
Source: Comparative Clinical Pathology - August 5, 2016 Category: Pathology Source Type: research

Xenograft of Mus musculus ovary in the uterus of Rattus norvegicus after induction of pseudopregnancy
AbstractXenograft of gonads such as ovarian tissue can preserve fertility in patients at risk of infertility after radiotherapy or chemotherapy and also is valuable in preserving fertility in mammalian species. The purpose of this investigation was to preserve mouse (Mus musculus) ovarian tissue after xenografting in uterine lumen of rat (Rattus norvegicus) immunoprivileged by pseudopregnancy. Ten adult female Balb/c mice and seven female and four male Sprague-Dawley rats were enrolled. Seven mice were selected for transplantation, and three mice were euthanized and their ovaries were removed as controls. Female Sprague-Da...
Source: Comparative Clinical Pathology - August 4, 2016 Category: Pathology Source Type: research

Investigating temporal changes and effects of elevation on the prevalence of a rickettsial blood parasite in red-backed salamanders ( Plethodon cinereus ) in Virginia, USA
Abstract Amphibians are host to a number of vector-transmitted blood parasites. One parasite for which there is little information is a recently described intracellular bacteria in the order Rickettsiales , found in red-backed salamanders ( Plethodon cinereus ) in the eastern USA. This parasite was observed in 16.7  % of individuals across three states in the summer of 2008. Here, we resampled one of the locations from the 2008 study (Mountain Lake, Virginia, where site-level prevalence was 22 %) to determine if the incidence of infection has changed in 5 years. We also examined red-backed salamanders at se...
Source: Comparative Clinical Pathology - July 29, 2016 Category: Pathology Source Type: research

Assessment of gastrointestinal helminths among house mice ( Mus musculus) caught in the north-west of Iran, with a special view on zoonotic aspects
The objective of this study was to assess parasitic infection and associated risk factors among house mice caught in West Azarbaijan, north-west of Iran, with a special view on zoonotic aspects. One hundred and fifty six captured rodents were individually anesthetized with ether and dissected. The whole gastrointestinal tract was excised and examined for the presence of parasites. Of 156 examined mice, 43 (27.6  %) were found positive for gastrointestinal parasite. The overall, three Nematoda species; Syphacia obvelata (11.5  %), Syphacia muris (1.9  %), and Aspicularis tetraptera (1.3  %) and two Ce...
Source: Comparative Clinical Pathology - July 28, 2016 Category: Pathology Source Type: research

Biebersteinia multifida DC a potential savior against testicular torsion-induced reperfusion injury
Abstract The study aimed to investigate the potential therapeutic effect of the methanolic extract of Biebersteinia multifida DC on ischemia –reperfusion injury induced by testicular torsion/detorsion in rats. Forty adult male Sprague-Dawley rats were randomly divided into four groups. Sham operation group, torsion/detorsion group plus saline, and torsion/detorsion groups plus 75 and 150 mg/kg doses of DC extract (administered intrape ritoneally 15 min before detorsion). Testicular ischemia was induced via keeping the left testis under 720° counterclockwise torsion for 2 h; afterwards, detorsion ...
Source: Comparative Clinical Pathology - July 27, 2016 Category: Pathology Source Type: research

Phylotyping and ColV plasmid-associated virulence genotyping of E. coli isolated from broiler chickens with colibacillosis in Iran
Abstract Avian pathogenic Escherichia coli (APEC) strains are responsible for significant losses to the poultry industry in many parts of the world. Although, many serotypes of E. coli are non pathogen, a relatively few number of serotypes carry virulence genes which make them damaging for chicken and other poultry. Previously, high prevalence of virulence genes carried on plasmid and/or chromosomal DNA has demonstrated in APECs obtained from different countries. The aim of the present study is characterization of E. coli isolated from broiler chickens with pericarditis and femoral head necrosis (FHN) associated with c...
Source: Comparative Clinical Pathology - July 27, 2016 Category: Pathology Source Type: research

PCR and PCR-RFLP genotyping of Staphylococcus aureus coagulase gene: convenience compared to pulse-field gel electrophoresis
Abstract Staphylococcus aureus belongs to the group of major contagious mastitis pathogens. We aimed to evaluate the usefulness and the convenience of the coagulase ( coa ) gene polymorphism to type S. aureus from bovine mastitis milk samples. A total of 22 S. aureus strains were collected from bovine cases of mastitis in four veterinary practices in the Rhone-Alpes region of France and were assessed using coagulase gene ’s polymerase chain reaction (PCR) followed by Alu I PCR-restriction fragment length polymorphism (PCR-RFLP). Coa gene PCR produced six profiles. The Alu I PCR-RFLP displayed nine profiles, givi...
Source: Comparative Clinical Pathology - July 26, 2016 Category: Pathology Source Type: research

A prospective comparison of brush cytology before and after biopsy in diagnosis of gastric malignancies
Abstract Gastric malignancies are the third most common causes of cancer-related death in the world. Here, we aimed to compare brush cytology before and after biopsy in the diagnosis of gastric malignancies. This prospective study included 74 cases referred to endoscopy clinic for suspected gastric lesions during 1  year. In 38 cases, brush cytology was done before and in 36 cases it was done after biopsy. All the cytology and biopsy slides were randomly coded and reviewed by a pathologist blindly without any knowledge of the surgical pathology result or clinical data of the patients. Diagnostic accuracy of b rush cy...
Source: Comparative Clinical Pathology - July 26, 2016 Category: Pathology Source Type: research

Evaluation of the hepato-protective potential of Sarcocephalus latifolius leaf methanol extracts in paracetamol-induced liver damage of mice
Abstract Sarcocephalus latifolius , the African peach, is one greatly cherished, eaten and widely distributed shrubs mainly in the closed woodland savannah, and has been used to treat diverse diseases in some African and Asia populations. In Southern Nigeria, ‘herbal doctors’ include its leaves in decoctions given to patients of varying degrees of suspected liver damage. However, there is no scientific substantiation of their claim and use in literature. To evaluate the possible hepato-protective activity of S. latifolius leaves extract in paracetamol-induced hepato-compromised animal model as information is ...
Source: Comparative Clinical Pathology - July 26, 2016 Category: Pathology Source Type: research

Breed and seasonal variations in erythrocyte osmotic fragility of goat kids raised in semi-arid savannah
In conclusion, breed and season dramatically influence the susceptibility of erythrocytes of kids to hypotonic haemolysis with heat stress during the HDS causing decrease in membrane integrity in both breeds of goats. However, sexual immaturity may b e responsible for lack of sexual dimorphism in EOF in both breeds. The use of mitigation techniques and prevention of additional stress factors such as weaning is recommended during the peak of the HDS. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - July 25, 2016 Category: Pathology Source Type: research

Immune-mediated hemolytic anemia in a hypereosinophilic dog with intestinal T-cell lymphoma
Abstract A 13-year-old, 21-kg, female, mixed breed dog was presented with multifocal skin rashes, abdominal distension, and vomiting for several days. Radiographic images displayed the small intestine thickening. On exploratory laparotomy, a wall thickness of the jejunum and ileum about 15  cm long, with an adhesive 1-cm-diameter nodular mass was found. Histopathologically, the mass was diagnosed as lymphoma. The lymphoid cells were positive for CD 3, but were negative for CD 79α on immunohistochemical staining. Hematological profiles revealed severe hypochromia, autoagglutination, and eosinophilic left shift. ...
Source: Comparative Clinical Pathology - July 23, 2016 Category: Pathology Source Type: research

Acute renal failure by use of dipyrone in Cavia porcellus
Abstract Renal failure is a condition that occurs when one or more functions of the kidneys are changed and in guinea pig (Cavia porcellus) its occurrence is uncommon but always lethal. This paper reports the occurrence of acute renal failure in a patient mistakenly treated with dipyrone for 5 days in a veterinary hospital of UNOESC/Xanxerê-SC, presenting apathy, anorexia and hematuria diagnosed through laboratory tests. The use of dipyrone generated an eminent intoxication condition and the specific knowledge of therapy and familiarity with exotic animal species is extremely important to avoid cases of...
Source: Comparative Clinical Pathology - July 18, 2016 Category: Pathology Source Type: research

Role of fibronectin in the process of human intestinal cell disruption during E. histolytica infection
We examined the mechanism using topology networks and functional analysis and the result showed links to 765 proteins, of which fibronectin was predominant in the network. Fibronectin interacts with ubiquitin conjugate, insulin-like factor binding protein-3, and integrin alpha chain-2 which play pivotal roles in maintaining cell cycle, survival, and apoptosis. Therefore, inhibition of the protein results in intestinal cells losing the ability to maintain homeostasis mechanism by which the disruption of intestinal cell occurs. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - July 18, 2016 Category: Pathology Source Type: research

Electron microscopic evaluation of milk thistle, chicory, and vitamin E effects on gentamicin-induced nephrotoxicity in rat
In conclusion, milk thistle, chicory, and vitamin E, individually, can reduce kidney damage caused by gentamicin, but they have a greater effect in reducing damage if used together. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - July 14, 2016 Category: Pathology Source Type: research

C hanges in serum biochemical parameters and oxidative stress biomarkers in grazing cattle
In this study, blood was collected from healthy cows kept in a pen (non-grazing) for 1 week after which they were subjected to grazing for another week, to determine the effect of grazing on serum biochemical parameters and oxidative stress biomarkers. Grazing significantly increased (p 
Source: Comparative Clinical Pathology - July 12, 2016 Category: Pathology Source Type: research

The growth kinetic, differentiation properties, karyotyping, and characterization of adipose tissue-derived stem cells in hamster
Abstract Multilineage properties of mesenchymal stem cells (MSCs) cause their contribution in repair of different tissue. Adipose tissue is considered a good source of MSCs because of its abundance and easy access. The aims of this study were to characterize and determine the differentiation properties and growth kinetics of adipose tissue-derived stem cells (AT-SCs) from Syrian hamster. Fat tissue was isolated from the abdominal and pelvic regions of the hamster and by using type I collagenase, and cells were isolated and cultured. AT-SCs were characterized by morphology, cell surface markers, and adipogenic and ...
Source: Comparative Clinical Pathology - July 12, 2016 Category: Pathology Source Type: research

The effect of hydroalcoholic extract of Mangifera indica on induced osteoarthritis of knee in male guinea pigs
Abstract Osteoarthritis (OA) is one of the top six diseases for which complementary and alternative medicine is used. Among the herbal products, mango could be regarded as a potential natural remedy for OA due to its anti-inflammatory characteristics. The aim of the present study was to evaluate the healing effect of mango on OA of the knee. Eighteen male guinea pigs were chosen, and anterior cruciate ligament transection was performed for them to induce OA for the animal models. Then, they were recruited in four treatment groups (n = 5): (a) oral treatment, (b) intra-articular injection treatment, (c) p...
Source: Comparative Clinical Pathology - July 11, 2016 Category: Pathology Source Type: research

D -dimer concentrations in dogs with kidney disease with or without protein-losing nephropathy
In conclusion, the detection of increased D-dimers in the context of impaired kidney function should be cautiously interpreted in the dog and should not be invariably assumed to imply the presence of a state of hypercoagulability. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - July 11, 2016 Category: Pathology Source Type: research

Point-of-care blood gases and critical care blood chemistry and haematology reference values in captive gyr falcons ( Falco rusticolus )
Abstract Blood samples were obtained from a total of 94 clinically normal captive gyr falcons (Falco rusticolus) divided into two groups. The first group comprised 44 gyr falcons (35 females and 9 males). These falcons were 5 to 6 months old and were destined for the sport of falconry. The second group comprised 50 gyr falcons, (26 females and 24 males). These falcons were 2 to 9 years old and were part of the captive breeding programme at the Falcon Department, Wildlife Division, Wrsan, Abu Dhabi, United Arab Emirates (UAE). The samples were obtained in order to determine blood gases and critical care b...
Source: Comparative Clinical Pathology - July 6, 2016 Category: Pathology Source Type: research

Neoplasia in flat head grey mullet, Mugil cephalus Linnaeus, 1758 (Perciforms: Mugilidae): two case reports of myxoinflammatory fibroblastic sarcoma and leiomyoma
Abstract The presence of cutaneous neoplasms is reported in two cases of wild female grey mullet (Mugil cephalus) from Pazhayar and Parangipettai fish landing centre, southeast coast of India. A gross morphological examination case 1 revealed obvious reddish white, solitary and fleshy appearance of tumour located on behind the right-side of the anal fin, and the overlying epidermis was ulcerated, hyperemic and hyperchromatic. The tumour had a gelatinous consistency and released jelly-like matters. The tumour-affected case 2 revealed well-circumscribed, solitary and reddish fleshy appearance of tumour located on th...
Source: Comparative Clinical Pathology - July 6, 2016 Category: Pathology Source Type: research

Sorghum stem extract modulates Na + /K + -ATPase, ecto-5 ′-nucleotidase, and acetylcholinesterase activities
This study sought to assess the antioxidant activity and effects of phenolic extract from sorghum stem ( Sorghum bicolor ) on some cholinergic (acetylcholinesterase (AChE)) and purinergic (Na + /K + -ATPase and ecto-5 ′-nucleotidase) enzymes associated with neurological conditions. Phenolic-rich extract was prepared using methanol: 1 N HCl (1:1, v / v ) mixture and characterized using high-performance liquid chromatography-diode array detector (HPLC-DAD). In vitro tests were used to investigate the effects of the phenolic extract on AChE, Na + /K + -ATPase, and ecto-5 ′-nucleotidase activities. Furthermore...
Source: Comparative Clinical Pathology - July 1, 2016 Category: Pathology Source Type: research

Histopathological evaluation and molecular detection of natural Iridovirus infection in cultured grouper fish in Malaysia
Abstract Iridovirus is the causative agent of grouper iridovirus infection (GIV), which causes severe epizootics resulting in large-scale mortalities and huge economic losses in cultured and marine fishes worldwide. The current study evaluated the gross and histopathological lesions, and molecular detection of GIV in naturally infected grouper farms in Malaysia. A total of 150 moribund fish showing different clinical signs, presented to the Aquatic unit were used for this study. The fish were necropsied and visceral organs (spleen, kidney, heart, liver, intestine, brain, eyes, gills, and some parts ...
Source: Comparative Clinical Pathology - June 28, 2016 Category: Pathology Source Type: research

Free-living ruddy ground dove ( Columbina talpacoti ): a report on leukocyte and weight values
Abstract The Columbina talpacoti species, a common type of bird in Brazil, is often the target of illegal trade or used as pets, which can lead to the threat of extinction as the number of individuals of the species decreases. As this is a rare species with unknown hematological parameters, this study aimed to describe the species’ leukocyte values and biometrics (weight). Twenty-five birds were captured at a private farm located in Guarapari, Espírito Santo, in southeastern Brazil. The capture and sampling were conducted in October 2014. The birds’ body mass was measured, and their blood was co...
Source: Comparative Clinical Pathology - June 23, 2016 Category: Pathology Source Type: research

Disturbance of thyroid function in canine ehrlichiosis and babesiosis associated with oxidative stress
Abstract Canine babesiosis and ehrlichiosis are endemic in India. The effect of these diseases on hematological and clinical biochemistry is well established; however, the association with thyroid function and oxidative stress is not known. Accordingly, we assessed the thyroid function and oxidative stress in the dogs with babesiosis (n = 8) and ehrlichiosis (n = 10) based on the blood smear examination. Non-infected dogs (n = 8) served as control. Assay of plasma free triiodothyronine (FT3) and free thyroxine (FT4) was done by enzyme-linked immunosorbent assay (ELISA); plasma malondi...
Source: Comparative Clinical Pathology - June 23, 2016 Category: Pathology Source Type: research

Fish bone versus fish demineralized bone matrix (vertebra) effects on healing of experimental radial defect in rat model
This study showed that the fish bone graft is effective in healing of the radial bone defect in rat. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 22, 2016 Category: Pathology Source Type: research

Prevalence of hepatitis B, hepatitis C, and human immunodeficiency virus infections among hemophilia patients in Shiraz, south of Iran
This study was conducted to assess the seroprevalence of HBV, HCV, and HIV infections among Iranian hemophilia patients. A cross-sectional study was conducted on 580 hemophilia patients (mean age ± SD = 27.11 ± 14.9) referred to the Namazi hospital, Shiraz University of medical sciences, Shiraz, Iran during July 2012 to August 2015. Blood samples were taken from the patients and tested for HBV surface antigen (HBsAg), antibody to HBV surface antigen (anti-HBs), antibody to HBV core antigen (anti-HBc), anti-HCV antibody (HCVAb), and anti-HIV antibody (HIVAb). The association of HB...
Source: Comparative Clinical Pathology - June 21, 2016 Category: Pathology Source Type: research

Anti-tumor effect of C-phycocyanin from Anabaena sp.ISC55 in inbred BALB/c mice injected with 4T1 breast cancer cell
This study investigated anti-tumor effect of C-phycocyanin from Anabaena sp.ISC55 on breast cancer in inbred BALB/c mice; 50 adult BALB/c inbred mice with injection of 4T1 breast cancer cell line were examined. Animals were classified into six groups of treatment and control. Two doses of phycocyanin were considered in the treatment groups; the main dose was 10-mg/kg body weight, and the second dose was 150-mg/kg body weight. Paclitaxel was used at a dose of 100.0-mg/kg body weight as comparative control drug. Microscopic examination on mammary tissue after 14 days in the treatment group with high dose of Anabaena phy...
Source: Comparative Clinical Pathology - June 16, 2016 Category: Pathology Source Type: research

Effects of dust particles on body weight and some haematological parameters in rats in Maiduguri metropolis, Nigeria
Abstract Maiduguri metropolis in Northern Nigeria is a typical Sahel region characterized by dry hot climate for a greater part of the year during which dust pollution is a major concern with regard to human and animal health. In order to understand some effects of prolonged dust exposure, this study investigated the effects of dust exposure on body weight and some haematological parameters of rats in Maiduguri metropolis. Sixty (60) rats of both sexes were used for the study. The rats were randomly divided into two groups of 30 rats each and designated groups I and II, being the experimental and control groups re...
Source: Comparative Clinical Pathology - June 1, 2016 Category: Pathology Source Type: research

Physicochemical and structure-activity properties of piroxicam—a mini review
Abstract The neutral bidentate ligand coordinated to the metal ion through pyridyl-N and carbonyl of the amide moiety causes protonation/deprotonation equilibria (tautomerism) of piroxicam. The penetration of piroxicam through blood–brain barrier (BBB) may be by redox chemical delivery system linking it to the lipophilic dihydropyridine carrier creating a complex with carboxylic acid that transverses the BBB. The complex is enzymatically oxidized to the conic pyridinium salt. Subsequent cleavage of the drug from the pyridinium carrier leads to sustained drug delivery in the brain parenchyma. Brain uptake of ...
Source: Comparative Clinical Pathology - May 24, 2016 Category: Pathology Source Type: research

Comparative efficacy of graded doses of diaminazine aceturate and fixed doses of iron dextran and vitamin B complex in mice infected with Trypanosoma brucei brucei
Abstract Trypanosomosis is a disease of both domestic animals and man. Trypanosomosis continues to be a menace in the livestock industry in Nigeria despite the agelong attempt to control the disease. The comparative effects of graded doses of diaminazine aceturate (DA) and fixed doses of iron dextran and vitamin B complex were experimentally investigated in the treatment of Trypanosoma brucei brucei-infected albino mice. A total of 40 albino mice were used and were randomly divided into eight groups of five mice each. All the mice in all the groups except group H were infected with T. brucei brucei, but group H se...
Source: Comparative Clinical Pathology - May 23, 2016 Category: Pathology Source Type: research

Experimental study on blood gases and acid-base parameter changes following oral administration of injectable enrofloxacin 5 % to the horses
This study showed that 3 weeks of oral administration of enrofloxacin 5 % had no significant effect on blood gases and electrolyte parameters even at high doses (15 mg/kg). This study showed the lack of drug effect on producing gastrointestinal mucosal ulcers and colic pain in the drug-treated horses. It was recommended that the drug enrofloxacin should not preferably be used in foals and also in mature horses for long duration, because of the possible joint disorder, and its usage should be with caution. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 21, 2016 Category: Pathology Source Type: research

Diagnostic efficacy of abattoir meat inspection for detecting bovine tuberculosis at Adama municipal abattoir, Ethiopia
Abstract Bovine tuberculosis (BTB) is endemic in Ethiopia and like other developing countries in Africa. Ethiopia has limited laboratory and other diagnostic facilities. Therefore, abattoir inspection still remains the only option for monitoring BTB prevalence in domestic animals. However, information regarding the efficacy of such routine abattoir (RA) inspection is limited. The present study was conducted on 500 slaughtered cattle heads, slaughtered at the Adama municipal abattoir, Ethiopia from December 2009 to April 2010 to elucidate the efficacy of RA inspection with respect to the detailed abattoir (DA) insp...
Source: Comparative Clinical Pathology - May 18, 2016 Category: Pathology Source Type: research

Serum anti-HSP27 antibody titers in patients with metabolic syndrome, with or without diabetes mellitus
Abstract Metabolic syndrome (MetS) is characterized by clustering of clinical, physiological, biochemical, and metabolic factors that are associated with an increased risk of cardiovascular disease (CVD) and type 2 diabetes mellitus. Immune response to heat shock protein 27 (Hsp27) has been suggested to be implicated in atherogenesis. We aimed to investigate the association between serum anti-Hsp27 antibody concentrations and type 2 diabetes in patients with MetS. This was a cross-sectional observational study on groups of MetS and healthy subjects. The population sample was derived from MASHAD STUDY, a national c...
Source: Comparative Clinical Pathology - May 16, 2016 Category: Pathology Source Type: research

Hematological reference values for stray colony cats of northern Italy
The objectives of this study are to determine hematological reference intervals (RIs) from a large population of stray cats in northern Italy, to establish whether published RIs for the general pet feline population are valid in stray cats and to evaluate the effects of age and sex on hematological parameters. Hematological data were analyzed retrospectively from the database of a trap-neuter-release program (performed in 2008–2010 in northern Italy) to generate normal RIs. RIs were determined and compared with established pet cat RIs according to the National Committee for Clinical Laboratory Standard guidelines and...
Source: Comparative Clinical Pathology - May 13, 2016 Category: Pathology Source Type: research

Biochemical profile of polo horses in training phase and those players of official competition
Abstract Training is essential to prepare the body for intense changes occurring in athletic performance. The effect of training depends on the duration, type, frequency, and intensity of athletic training. The aim of this study was to evaluate the profile of proteins, metabolites, minerals, and enzymes of polo horses in training and those that participate regularly in official competitions. Blood samples were collected from 82 thoroughbred horses. A single collection was performed in the morning, before training the horses. They were divided into two groups: group 1 consisted of training horses, which have not ye...
Source: Comparative Clinical Pathology - May 12, 2016 Category: Pathology Source Type: research

Relationships between follicular fluid steroid concentrations and uterine infections in ovarian cystic cows
Abstract The present study aimed to determine the relationships among uterine bacterial infection, ovarian cysts, and endometritis. A total of 21 genital tracts with ovarian cysts were collected from the abattoir. Cystic fluids were aspirated from ovarian cysts (follicular cysts, n = 10; luteal cysts, n = 11) and eight large follicles (
Source: Comparative Clinical Pathology - May 10, 2016 Category: Pathology Source Type: research

Reference intervals for hematological and blood biochemistry reference values in healthy mules and hinnies
In this study, hinnies and mules showed results that were closer to those of horses than those of donkeys. Some differences recorded in hinnies may be related to age: RBC, WBC, MCH, MCV, eosinophils, magnesium, total bilirubin, creatinine, and AST. Findings may help establish new, relevant hematological and biochemical parameters which may prevent medical misdiagnosis. Additional research is needed with larger populations of healthy mules and hinnies. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 10, 2016 Category: Pathology Source Type: research

Biochemical and morphological changes in rats exposed to platinum nanoparticles
This study evaluated the toxicity potential of platinum (Pt) nanoparticles in Wistar rats. Wistar rats weighing between 180 and 193 g were randomly assigned into four groups. Animals in group 1 served as the control and received distilled water. Those in groups 2, 3, and 4 were administered with 10, 50, and 100 mg/kg body weight of Pt nanoparticles, respectively. At the end of treatments, the rats were fasted for 24 h and sacrificed under mild anesthesia. The blood and vital organs were collected and used for the biochemical and histopathological examinations. Pt nanoparticles caused significant alterations ...
Source: Comparative Clinical Pathology - May 6, 2016 Category: Pathology Source Type: research

Hematology and serum biochemistry values in greylag geese ( Anser anser ) in Southeast Iran
Abstract Greylag geese (Anser anser) migrate to the different parts of Iran including Sistan region, southeastern Iran. It breeds in Hamun lake in Sistan region. The current study was conducted to establish normal values of serum biochemistry and hematology parameters in greylag geese. Nineteen serum biochemistry and nine hematology parameters were measured in 31 healthy adult greylag geese from both genders (16 males and 15 females). With the exception of the high-density lipoprotein (HDL) and low-density lipoprotein (LDL), serum biochemistry and hematology values did not vary significantly by sex. Graylag geese ...
Source: Comparative Clinical Pathology - May 5, 2016 Category: Pathology Source Type: research

Ethanol effects on histobiochemical parameters of suckling pups borned from alcoholic rat mothers
This study was designed to investigate the effects of consuming different doses of ethanol during lactation on enzymatic and tissue changes and growth indices in suckling rat pups. Forty-five lactating Wistar rats with nine lactating pups each were randomly allocated to three treatment groups. The two treatment groups received 2 and 4 % v/v ethanol, while the third group was the control on distilled water for 24 days. On day 25 after birth, 30 newborn rats were randomly selected from each group and serum activity of liver enzyme markers, lactate dehydrogenase, blood urea nitrogen, creatinine, and creatine phospho...
Source: Comparative Clinical Pathology - May 3, 2016 Category: Pathology Source Type: research

Development of RFLP-PCR and simple multiplex PCR assays for detection and differentiation of two species of hemotropic mycoplasmas in naturally infected dogs
In this study, the occurrence and differentiation of both causative agents in dogs that originated from southern Iran were investigated by employing restriction fragment length polymorphism (RFLP)-PCR and simple multiplex PCR methods based on RNase P RNA (rnpB) and 16S rDNA markers, respectively. The results showed that the prevalence of Mhc and CMhp among 100 examined samples were 6.0 and 4.0 %, respectively. Both PCR assays provide valuable molecular tools for the differentiation of CMhp and Mhc DNA in canine blood samples compared to 16S rDNA sequencing results. Also, sequence analysis of the genes (rnpB and 16S rD...
Source: Comparative Clinical Pathology - April 26, 2016 Category: Pathology Source Type: research

The efficacy of zymosan in pro-inflammatory cytokine production by peritoneal macrophages of melanoma-bearing mice
Abstract Zymosan, a polysaccharide derived from yeast cell wall, has been widely applied as a macrophage-activating factor. Application of immunomodulatory compounds as cancer therapeutics has emerged in recent years. The goal of this study was to assess the effect of zymosan in the modulation of pro-inflammatory cytokines in melanoma-bearing mice. C57BL/6 mice were divided into four groups of seven mice including zymosan (Z group), melanoma (M group), zymosan treated melanoma-bearing mice (ZM group), and control (C group). Mice were treated with zymosan (10 μg) for four continuous days. At the end of the ...
Source: Comparative Clinical Pathology - April 26, 2016 Category: Pathology Source Type: research

Experimental infection with Trypanosoma evansi in rams: presence of parasite in semen, investigation of sexual transmission, hematological, and biochemical alterations
Abstract The aims of this study were to investigate Trypanosoma evansi venereal transmission in ewes and its presence in ram’s semen and hematological and biochemical alterations. Three adult rams and six ewes were used as follows: one uninfected ram was used as control (R1), and two rams (R2 and R3) were intravenously infected by T. evansi trypomastigotes. Semen samples were collected weekly for PCR analyses that have detected the presence of T. evansi. Blood samples were collected, and it was observed that the number of red blood cells and hemoglobin concentration were reduced in infected rams on day 14 po...
Source: Comparative Clinical Pathology - April 23, 2016 Category: Pathology Source Type: research

Pathology of emphysematous reticulitis in cattle: report of two cases and comparative overview of similar or identical conditions
Abstract Inflammatory forestomach disorders other than those of viral, bacterial, mycotic, parasitic, chemical, or plant poisoning origin are uncommon in the bovine species. Spontaneous focal emphysematous lesions were grossly identified in the reticulum—the second part of the forestomach—of two adult Holstein-Friesian cattle (one 2-year-old steer, the other 3-year-old dairy cow). Both cattle, which appeared to be clinically normal without evidence of apparent forestomach dysfunction or other significant abnormalities on physical examination, were slaughtered conventionally for human consumption. At po...
Source: Comparative Clinical Pathology - April 22, 2016 Category: Pathology Source Type: research

Pre- and post-partum seric biochemical variables of Lacaune ewes naturally infected by gastrointestinal parasites
Abstract The main objective of the present study was to evaluate the biochemical profile of the Lacaune ewes naturally infected by gastrointestinal parasites in the pre- and post-partum period. Ewes with similar age and weight, primiparas in the final stage of gestation, totaling 24 animals were selected. Sampling days were 10 and 2 for pre-partum and 2, 6, and 12 days for post-partum to evaluate total proteins, albumin, globulin, cholesterol, triglycerides, and urea levels, in addition to thiobarbituric acid reactive substances (TBARS), reactive oxygen species (ROS), and glutathione S-transferase (GST). Feca...
Source: Comparative Clinical Pathology - April 16, 2016 Category: Pathology Source Type: research

VIP-loaded PLGA as an anti-asthma nanodrug candidate
Abstract Poly lactic-co-glycolic acid (PLGA), a biodegradable polymer, can effectively protect encapsulated peptides from enzymatic degradation. PLGA was approved by FDA as a safe drug delivery system suitable for inhalation administration. Vasoactive intestinal peptide (VIP), a 28-amino-acid peptide, displays anti-inflammatory and anti-spasmodic effects, which can be considered as a new therapeutic option to control and treat asthma. Because of in vivo enzymatic degradation of VIP including in the lung, there is a need for an applicable delivery system. In light of this, the purpose of this study was to prepare V...
Source: Comparative Clinical Pathology - April 15, 2016 Category: Pathology Source Type: research

The effect of Iresine herbstii Hook on some haematological parameters of experimentally induced anaemic rats
Abstract Iresine herbstii is fed to livestock in South-eastern Nigeria in the belief that it boosts their blood supply. The aim of this work is to study the effect of the methanolic extract of I. herbstii Hook on some blood parameters of experimentally induced anaemic albino rats. For acute toxicity study, graded doses of the methanolic leaf extract of I. herbstii at 10, 100, 1000, 1600, 2900 and 5000 mg/kg were administered orally to rats randomly allotted to six groups of three animals each and signs of toxicity were observed for 24 h. To evaluate its haematological effects, 100, 200, an...
Source: Comparative Clinical Pathology - April 15, 2016 Category: Pathology Source Type: research

Abnormal gene expression leads to poor prognosis in breast cancer patients in Bihar
Abstract With its highest mortality rate, the breast cancer outruns other gynecological cancers. For a long time, the breast cancer was associated with poor prognosis. But with the advent of analysis of expression level of different protein receptors (ER/PR and Her-2/neu) with the help of immunohistochemistry (IHC) has ushered us to a new era of treatment of cancer called immunotherapy. Tissues sampled from 167 breast cancer patients at pathology labs of Mahavir Cancer Institute & Research Center, S S Hospital and Research Institute and NMCH, Patna, Bihar, India, were processed by a routine protocol. ...
Source: Comparative Clinical Pathology - April 14, 2016 Category: Pathology Source Type: research

Removal of spermatogonial stem cells (SSCs) from in vitro culture modulates testosterone synthesis in bovine
Abstract Niche cells, encompassing spermatogonial stem cells (SSCs) and controlling their development are believed to have a reciprocal communication with SSCs. The present study was conducted to evaluate the impact of the elimination of SSCs on testosterone production and genes contributing to testosterone synthesis. Following isolation, bovine testicular cells were cultured for 12 days on extracellular matrix-coated plates. In the germ cell-removed group, the SSCs were removed from the in vitro culture using differential plating; however, in the control group, no intervention in the culture was performed. T...
Source: Comparative Clinical Pathology - April 13, 2016 Category: Pathology Source Type: research

Pustular rumenitis associated with epithelial akeratosis, dyskeratosis, and parakeratosis in steers: report of two cases
Abstract Although occurrence of inflammatory lesions of the rumen due to a variety of factors is relatively common in the bovine species, there are a limited number of actually published reports on ruminal pustules. In order to deepen knowledge of the pathological process involving inflammatory rumen disorders in this species, histopathological investigations were performed on ruminal pustules in two 2-year-old steers which were slaughtered conventionally for food. On postmortem gross examination of the steers’ rumen, the mucosal surface of the ventral sac revealed focal areas of irregular arrangement, disco...
Source: Comparative Clinical Pathology - April 8, 2016 Category: Pathology Source Type: research