Effects of electrofishing on stress response of wild carp ( Cyprinus carpio , L.)
AbstractElectrofishing is commonly used by fishery scientists for collecting fishes. During such work, fishes are often exposed to air for several minutes which lead to deleterious impacts. The purpose of the current paper was to compare hematological and biochemical parameters of wild carp when electrofishing device was applied. Hematology and serum biochemical analyses were performed. The result showed significant differences (p 
Source: Comparative Clinical Pathology - February 27, 2018 Category: Pathology Source Type: research

Histomorphometric and ultrasonographic evaluations of the abomasum in sheep
In this study, the samples were collected from fundus, body, and pyloric regions of the abomasum from 10 healthy sheep and fixed in 10% neutral buffered formalin. All samples were processed through routine paraffin embedding technique, cut at 6  μm, and stained with hematoxylin and eosin. Histomorphometry of samples was done by Olympus microscope (BX1) and camera (DP25). Ultrasonography of the abomasum wall was performed with 8-MHz probe. Statistical analysis showed that there is no significant correlation between ultrasonography and his tomorphometry. It seems possible that these results are due to unwanted change...
Source: Comparative Clinical Pathology - February 26, 2018 Category: Pathology Source Type: research

Prognostic relevance of CD69 expression in B cell chronic lymphocytic leukemia
This study aimed to determine the prognostic impact of CD69 expression in B cell chronic lymphocytic leukemia (CLL). The study included 153 B cell chronic lymphocytic leukemia (B-CLL) recruited to Mansoura Oncology Center. Cellular CD69 expression was determined by multicolor flow cytometry in parallel with PCR amplification and sequencing analysis to mutational status of immunoglobulin variable heavy chain (IgVH). The number of CLL patients positively expressed CD69 was 47 out of 153 (30.7%). There is significant association between CD69-positive expression and unmutated IgVH and between CD69 negative expressions with mut...
Source: Comparative Clinical Pathology - February 26, 2018 Category: Pathology Source Type: research

Concurrent infection of Maedi-Visna with lung worms and pulmonary adenomatosis in sheep kept in Debre Berhan Sheep Improvement Station, Central Ethiopia
AbstractThe study was conducted with the objectives of determining the possible causes of pneumonia in the sheep and to characterize the microscopic lesions of concurrent infection of Maedi-Visna with lung worms and other infectious pneumonia. Detailed gross and microscopic examinations were employed on 35 Maedi-Visna seropositive sheep. Out of 35 sheep slaughtered and examined for gross lesions on their lungs, lung worms, fibrinous adhesions, and mottling on the cranioventral lobes were observed with decreasing order of frequency. Histopathologically, the pneumonic lungs (n = 35) revealed Maedi (100%), Maedi-V...
Source: Comparative Clinical Pathology - February 26, 2018 Category: Pathology Source Type: research

Innate immunological responses of Asian sea bass, Lates calcarifer (Bloch, 1790) for experimentally challenged Aeromonas hydrophila infection
AbstractModulation of innate immunity was determined in theAeromonas hydrophila-challenged Asain sea bass,Lates calcarifer. The haemato-immunological parameters were studied in blood serum. Blood was sampled at 3, 6, 12, and 24th  h post-challenged infection and control. Histopathological examination of most important organs (ventral musculature tissue, kidney and liver) was studied in the infected fish. The present study results indicated that at early stages of infection, TEC, Hb, PCV, MCV, MCH, and MCHC were not altered significantly, whereas in TLC, the values were fluctuated. Lysozyme, NBT, and protein were signi...
Source: Comparative Clinical Pathology - February 24, 2018 Category: Pathology Source Type: research

The effect of ( Humulus lupulus L.) extract on blood factors in male rat
AbstractHumulus lupulus L. is a plant from cannabinaceac family which is grown in Iran as well as most regions round the world. The present study aimed at examining effects of hydroalcoholic extract ofHumulus lupulus L. hops on blood pictures in male rats. Rats were randomly assigned to seven groups of ten, including one control group (no treatment). All of the six groups under the study were exposed 7  weeks to 25% tween 80° solution (1 ml/kg/day), olive oil (1 ml/kg/day), hydroalcoholic extract ofHumulus lupulus L. (H) at 50, 150, or 450  mg/kg/day, as well as Di Ethyl Stilbestrol (DES) (100 ...
Source: Comparative Clinical Pathology - February 21, 2018 Category: Pathology Source Type: research

Hematological and plasm biochemical values for Rhinogobio ventralis in the Yangtze River, China
This study aimed to establish a “normal” hematological profile for theRhinogobio ventralis and to provide the basis for future comparative surveys. The hematological and plasma biochemical parameters were measured in the cyprinid species,R. ventralis, collected from the upper reaches of the Yangtze River. The concentrations of red blood cell count, white blood cell count, hematocrit, hemoglobin, mean corpuscle volume, mean corpuscle hemoglobin, Cl−, Ca2+, creatinine, urea, glucose, plasma total protein, plasma albumin, globulins, triglyceride, cholesterol, alanine aminotransferase, aspartate aminotransfer...
Source: Comparative Clinical Pathology - February 21, 2018 Category: Pathology Source Type: research

Perturbations of erythrocyte membrane integrity by subchronic low-level lead exposure in New Zealand white rabbits
AbstractLead (Pb) poisoning has been recognised as a clinical condition since ancient times. The anaemia frequently observed in Pb poisoning is thought to result from the shortening of erythrocytes, life span and inhibition of key enzymes of haem synthesis [viz. delta aminolevulinic acid dehydratase (ALAD), pyrimidine 5 ′-nucleotidase type 1 (P5N-1) and ferrochelatase]. However, the exact mechanism by which Pb shortens erythrocyte life span is still debatable. In the present study, the effect of ingested Pb on erythrocyte membrane sialic acid content and osmotic fragility of New Zealand White (NZW) rabbits was st udi...
Source: Comparative Clinical Pathology - February 20, 2018 Category: Pathology Source Type: research

Effect of age, breed and sex on haematological and blood biochemical parameters in helmeted guinea fowl ( Numida meleagris )
This study evaluated the effect of age, breed and sex on haematological and blood biochemical parameters in helmeted guinea fowl. Haemoglobin concentrations increased (P 
Source: Comparative Clinical Pathology - February 19, 2018 Category: Pathology Source Type: research

Recombinant VP2 expressed in baculovirus and adjuvanted with TIR-TLR7: a vaccine candidate against infectious bursal disease virus
AbstractCurrently, a variety of immunostimulatory molecules are used as adjuvants in combination with poor immunogenic recombinant infectious bursal disease virus (IBDV) vaccine antigen. TIR domain of Toll-like receptors (TLRs) triggers the interleukin-1 (IL-1) signaling cascade which plays a critical role in immune responses against infectious agents. For this study, the bacmid DNA pBac-VP2 of a field isolate was generated and transfected to Sf9 insect cells to produce recombinant VP2 (rVP2). In addition, based on in silico analysis, the cDNA encoding the conserved TIR domain of TLR7 (TIR-TLR7) contain the IL-1 receptor w...
Source: Comparative Clinical Pathology - February 19, 2018 Category: Pathology Source Type: research

Ovarian morphology and development of 3 to 8  months old African catfish, Clarias gariepinus
AbstractThirty female African catfish of 3, 4, 5, 6, 7, and 8  months (n = 5 per age) cultured in south-east Nigeria were used to investigate ovarian morphology and development using gross anatomical and histological techniques. The results illustrated that the gonadosomatic indices of the African catfish peaked at 7 months of age (20.05 ± 1.45). Histologicall y, the ovaries of 3-month-old fish exhibited only pre-vitellogenic oocytes, while 4- to 8-month-old fish showed all stages of oocyte development, with predominance of pre-vitellogenic at 4 and 5 months old, and mature...
Source: Comparative Clinical Pathology - February 17, 2018 Category: Pathology Source Type: research

Amelioration of renal structural changes in STZ-induced diabetic mice with ethanolic extract of Allium saralicum R.M. Fritsch
AbstractThe purpose of this study was to assess the hypoglycemic and protective activities of the ethanolic extract ofAllium saralicum (AS) in streptozotocin (STZ) induced diabetic mice. Diabetes was experimentally induced by intraperitoneal injection of STZ (60  mg/kg bw) in 35 mice. Fasting blood glucose (FBG) levels were measured everyday using glucometer strips. Mice with plasma glucose level> 250 mg/dL were considered diabetic. After 3 days, they were divided randomly into five groups. Groups 1 and 2 served as non-diabetic and untreated diabetic controls, respectively. Group 3 received 0.5 ...
Source: Comparative Clinical Pathology - February 17, 2018 Category: Pathology Source Type: research

Hematological and iron-related parameters in a dual-purpose local cattle breed compared to the specialized Italian Friesian breed during transition and lactation periods
AbstractCattle genetic selection has resulted in cows more predisposed to physiological diseases and immunological disorders. The aim of this study was to compare hematological and iron-related parameters during transition and lactation between the dual-purpose local cattle breed Modenese (MO) and the Italian Friesian (IF), housed together under identical conditions of feeding and management. Blood samples from 25 pluriparous lactating cows, 11 MO and 14 IF, were collected at different physiological status: ante-partum (a.p.), early post-partum (p.p.), and lactation. Significant differences were observed during all three p...
Source: Comparative Clinical Pathology - February 16, 2018 Category: Pathology Source Type: research

Co-administration of artemisinin and Ricinodendron heudelotii leaf extract —effects on selected antioxidants and liver parameters in male Wistar rats
This study assessed the effect of co-administration of artemisinin andRicinodendron heudelotii extract on certain liver and antioxidant indices in rats. Four groups containing ten rats each were administered distilled water (group A), artemisinin only (group B), artemisinin withR.heudelotii extract (group C), andR.heudelotii extract only (group D). Serum biochemical values and antioxidant parameters were determined using standard methods respectively. The results revealed that the total protein level increased significantly (p 
Source: Comparative Clinical Pathology - February 16, 2018 Category: Pathology Source Type: research

Renaut bodies in the hind limb nerves of cattle with downer cow syndrome
AbstractRenaut bodies are loosely textured, whorled, cell-sparse structures that are located in the sub-perineurial area of peripheral nerves. Renaut bodies have been previously recognized in human beings and some animal species. Pathogenesis of the structures is not fully understood. In the present study, peripheral nerves of four adult Holstein-Friesian dairy cows affected with the downer cow syndrome were investigated histopathologically. The animals were culled 3  days (one case), 12 days (one case), or 11 days (two cases) after the onset of clinical signs indicative of motor dysfunction, such as hind li...
Source: Comparative Clinical Pathology - February 16, 2018 Category: Pathology Source Type: research

Molecular detection of Leishmania species in northeast of Iran
AbstractTwo known types of cutaneous leishmaniasis (CL) including zoonotic CL due toLeishmania major and anthroponotic CL due toLeishmania tropica are prevalent in 14 of 22 countries located in the Eastern Mediterranean region including Iran. According to existing data, CL is endemic in Sabzevar City (northeast of Iran) and, because of the climatic conditions in this semi-desert region, is suitable for living vector/reservoir hosts of infection. The aim of our study was to identify the recent status of CL causative species in rural areas of Sabzevar County. Suspected patients of CL who were referred to health centers in su...
Source: Comparative Clinical Pathology - February 16, 2018 Category: Pathology Source Type: research

Malignant peripheral nerve sheath tumor in a cat: cytological, histopathological, and immunohistochemical aspects
AbstractThe malignant peripheral nerve sheath tumor is an uncommon tumor in cats. Cytological preparations can be used to diagnose such neoplasia, being a useful tool to characterize a spindle cell tumor presenting several criteria of malignancy. Histopathological examination is required for a definitive diagnosis, complemented by immunohistochemical techniques. This paper describes a case of malignant peripheral nerve sheath tumor in a cat. The diagnosis of mesenchymal neoplasm with high pleomorphism was suggested by cytology, performed through fine needle aspiration. Thereafter, the nodule underwent surgical excision, hi...
Source: Comparative Clinical Pathology - February 15, 2018 Category: Pathology Source Type: research

Haemocytomorphometric variation in different ages of Japanese quail, Coturnix coturnix japonica (Temminck and Schlegel, 1849)
AbstractThe investigation focuses to assess the haematological and cytomorphometric variation in Japanese quail (Coturnix coturnix japonica) at different age groups with sexual dimorphism. Two millilitres of blood from 60 individual birds, comprising 10 males and 10 females from three age groups, was collected from ulnar vein of Japanese quail. Haematological and morphometric values of the blood samples were determined by using standard protocols. For haematology and morphometry, three age groups were considered, i.e., chick (0 –3 weeks), grower (4–5 weeks), and adult (above 5 weeks), respectivel...
Source: Comparative Clinical Pathology - February 14, 2018 Category: Pathology Source Type: research

Potential pharmaceutic effect of Shilajit (mumie) on experimental osteoarthritis in rat
AbstractOsteoarthritis (OA) is a worldwide joint disease with clinically loss of motion and pain in human. The conventional treatments are associated with essential side effects. In folk medicine, Shilajit is applied for treatment of arthritis and bone fractures. The present study evaluated effect of Shilajit on the osteoarthritis in rat model. Thirty-six adult male rats were randomly divided into two groups including OA and treated with Shilajit groups. OA was induced by injection of monosodium iodoacetate in the articular space of femorotibial joint. Aqueous extract of Shilajit was given to the treatment group by gavage ...
Source: Comparative Clinical Pathology - February 14, 2018 Category: Pathology Source Type: research

Biochemical and ultrasonographic evaluation of exertional rhabdomyolysis and myositis in draft horses
AbstractThe purpose of this study was to evaluate the biochemical alterations in horses with exertional rhabdomyolysis (ER) and those with myositis and to characterize the progressive changes of both diseases by using ultrasonography (US). For this reason, 40 horses with various symptoms of musculoskeletal disorders were studied. Based on the competent case history, clinical symptoms, and in combination with clinicopathological findings as well as ultrasonographic alterations, the investigated horses were allocated into two main groups: ER-group (n = 7) and myositis group (n = 33). Blood samples...
Source: Comparative Clinical Pathology - February 14, 2018 Category: Pathology Source Type: research

Quercetin, rutin, and their combinations modulate penile phosphodiesterase-5 ′, arginase, acetylcholinesterase, and angiotensin-I-converting enzyme activities: a comparative study
In conclusion, our fi ndings suggest that the combination of quercetin and rutin is more potent than their individual effect. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 13, 2018 Category: Pathology Source Type: research

Effect of traumatic reticulitis and allied syndromes on blood constituents in buffaloes
This study was aimed to evaluate the effect of traumatic reticulitis and other affections on some blood variables in buffaloes. Female buffaloes (n = 62) were divided into eight groups: control, non-penetrating reticular foreign bodies (NPRF), traumatic reticulitis (TR), traumatic reticuloperitonitis (TRP), traumatic reticuloperitonitis with peri-reticular abscess (TRP + PA), traumatic reticuloperitonitis with vagus indigestion (TRP + VI), diaphragmatic hernia (DH), and traumatic pericarditis (TP). Results revealed a significant decrease in RBC count in cases of NPRF, TR, TRP, TRP + PA, and TP. Platelet count...
Source: Comparative Clinical Pathology - February 13, 2018 Category: Pathology Source Type: research

Genetic characterization and phylogenetic analysis of hemotrophic mycoplasmas in camel ( Camelus dromedarius )
AbstractSo far, a number of hemotrophic mycoplasma (HM) species have been reported from different animals, mostly based on the 16S rDNA gene sequence analysis. Although previous studies have describedCandidatus Mycoplasma haemolamae from alpaca (Lama pacos), llama (Lama glama), and vicuna (Lama vicugna) in Camelidae, data on the causative agent(s) of hemotrophic mycoplasmosis or hemoplasmosis are insufficient in dromedary camel (Camelus dromedarius). The purpose of the present molecular study was to investigate hemotrophic mycoplasmosis in Iranian dromedary camel. Accordingly, a total number of 100 EDTA-anticoagulated bloo...
Source: Comparative Clinical Pathology - February 13, 2018 Category: Pathology Source Type: research

Chronic immune thrombocytopenia. Egyptian experience
In this study, we reviewed the demographic features of thrombocytopenic Egyptian patients. We also analyzed the role of T cell in chronic immune cases. IL-12, IL-35, IL-17, and TGF- β were measured by ELISA. The median age at the time of diagnosis was 30 years and its range was 14–70. The median platelet count at the time of diagnosis was 15 × 109/L. Regarding treatment and follow-up, there was an indication for treatment in 96% of patients. Of the 150 ITP patients who were given first-line therapy (corticosteroid 1  mg/kg/day PO), there was a complete response (CR) in 40.3% while 5...
Source: Comparative Clinical Pathology - February 13, 2018 Category: Pathology Source Type: research

Assessment of two abdominal mass closure methods using fishing line in rabbit model: clinical and pathological study
This study has demonstrated that fishing line, as suture material, is suitable for mass abdom inal closure in rabbit model. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 9, 2018 Category: Pathology Source Type: research

Effects of atorvastatin on myocardial oxidative and nitrosative stress in diabetic rats
AbstractFree radicals play a pivotal role in many pathophysiological states, such as myopathies. Atorvastatin is a known cholesterol-lowering agent with many pleiotropic actions including antioxidant properties. However, the impact of atorvastatin on myocardial oxidative stress is not well known. The aim of this study was to evaluate the role of atorvastatin in improving diabetes-induced oxidative stress in the myocardium. Male Wistar rats weighing 20 –25 g were randomly divided into four groups as normal, normal-treated, diabetes, and diabetes-treated. Induction of diabetes was performed by a single dose of str...
Source: Comparative Clinical Pathology - February 8, 2018 Category: Pathology Source Type: research

The study on rate of morbidity of fungal infections exiting in educational hospitals: Iran
AbstractWhereas hospitals have been assumed as the sites for spreading infection at level of world health, therefore, the current descriptive study has been conducted on 248 inpatients and outpatients who were suspected to fungal infection or disease. Samples were collected from several wards along with radiographic and endoscopic analyses in three hospitals, including urine, blood, skin, nail, wound (burning, surgery, eye and injury), lung, phlegm, ascites, bronchoaveolar lavage, (cerebrospinal, abdominal, and knee) fluids, peritoneum, fistula, ear tests and biopsy and sent to reference mycological center. The fungal spec...
Source: Comparative Clinical Pathology - February 7, 2018 Category: Pathology Source Type: research

Investigation of histopathological changes caused by adult stage of Linguatula serrata in dog
AbstractLinguatula serrata, known as tongue worm, is an anomalous ubiquitous parasite. This parasite inhabits the canine respiratory system as its final host. The discharged eggs cause to contamination of many plants that are consumed by feeder animals including human causing visceral and nasopharyngeal linguatulosis that is known as “Marrara syndrome.” This research was conducted for the first time to investigate the distribution and pathological findings ofL. serrata adult in nasal cavity of road-killed dogs in Uremia, Iran. For doing so, 52 dogs were inspected. During the necropsy;L. serrata adult was seen i...
Source: Comparative Clinical Pathology - February 4, 2018 Category: Pathology Source Type: research

Prevalence and antifungal susceptibility of mycotic agents isolated from skin lesions of horses in Enugu State, Nigeria
This study has provided baseline information which includes sex and seasonal distributions of the fungal agents associated with skin lesions in trade horses in Obollo-Afor area of Enugu State. Antifungal susceptibility results shows that voriconazole and nystatin are the drugs of choice in the treatment of dermatomycoses of horses in Nigeria. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 3, 2018 Category: Pathology Source Type: research

A novel herbal beverage from Moringa oleifera , Dennettia tripetala , and Citrus sinensis abates Bonny light crude oil-induced oxidative injury in rat brain and liver
AbstractA novel herbal beverage (HB) was formulated from blends ofMoringa oleifera,Dennettia tripetala, andCitrus sinensis and investigated for its antioxidative protective effect against Bonny light crude oil (BLCO)-induced oxidative injury in rat brain and hepatic tissues. Male albino rats were orally exposed to BLCO (800  mg/kg bodyweight) for 7 days, then treated with HB (200 and 400 mg/kg bodyweight) for another 7 days. Reduced glutathione (GSH) and malondialdehyde (MDA) levels were depleted and elevated respectively in both tissues on exposure to BLCO, which was (p 
Source: Comparative Clinical Pathology - February 2, 2018 Category: Pathology Source Type: research

Potential of the ImageJ software in the differentiation of various types of canine lymphadenopathies with fine needle aspiration cytology (FNAC)
This study was designed to evaluate the potential of the ImageJ software in the differentiation of various types of lymphadenopathies with fine needle aspiration cytology (FNAC). For this purpose, the samples of enlarged mandibular lymph node were selected from 46 dogs suspected of lymphadenopathy and 10 healthy dogs as a control. The target tissues were obtained from the animals by fine needle aspiration and lymph node removed by surgery. The samples were then prepared for cytological and histological study. Finally, the ImageJ software was used to evaluate the quantitative nuclear morphometric parameters. The data obtain...
Source: Comparative Clinical Pathology - February 2, 2018 Category: Pathology Source Type: research

Effect of powdered seed of Nigella sativa administration on sub-chronic and chronic lead acetate induced hemato-biochemical and histopathological changes in Sprague Dawley rats
This study hypothesized that theN. sativa (NS) administration will ameliorate the deleterious effects of chronic PbAC toxicity in rats. A total of 75 Sprague Dawley rats were divided into three groups of 25 rats, and each group was further sub-divided into five groups of five rats each. Group 1 rats (negative control) were given distilled water, group 2 (positive control; PC) were given 10  mg/kg of lead acetate (PbAC) daily, and groups 3 (T1), 4 (T2), and 5 (T3) were each given 10 mg/kg of PbAC followed by graded concentrations of powdered seeds of NS; 100, 150, and 200 mg/kg, respectively. Five rats in eac...
Source: Comparative Clinical Pathology - February 2, 2018 Category: Pathology Source Type: research

Bracken fern toxicity and its associated clinicopathological effects in humans and animals: a review
In conclusion, special attention should be given to this plant by toxicologists, botanists, veterinarians, toxicopathologists, oncologists and public health specialists. The human populace, farmers inclusive, should be educated about the public health significance of this plant. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 2, 2018 Category: Pathology Source Type: research

Detecting endomysial and tissue transglutaminase antibodies in patients with giardiasis
AbstractCeliac disease is a genetic disease diagnosed to be associated with chronic intestinal inflammation. Endomysial and tissue transglutaminase antibodies are the best serum markers for celiac disease (CD) diagnosis. This research aimed to determine the levels of endomysial and tissue transglutaminase antibodies in patients with giardiasis. Forty cases of giardiasis were selected among the referees to Milad Hospital in Tehran, as well as a children ’s hospital and some health centers in Karaj. Euro-immune IgA immunofluorescence and ELISA were utilized to measure their titers of endomysial and tissue transglutamin...
Source: Comparative Clinical Pathology - January 30, 2018 Category: Pathology Source Type: research

Growth performances, survival rate, and biochemical parameters of Nile tilapia ( Oreochromis niloticus ) reared in water treated with probiotic
AbstractThe growth performances, survival rate, and biochemical parameters of Nile tilapia (Oreochromis niloticus) reared in water treated with probiotic were investigated. Samples of 225 Nile tilapia (5.8  ± 0.10 g) were reared for 90 days in three different treatments with three replications as the control group (no probiotic), and two test groups with probiotics consisting of yeast (Saccharomyces cerevisiae) (5.6  × 108 cfu g−1) andBacillus spp. (B. subtilis,B. megaterium, andB. licheniformis) (1  × 106 cfu g−1) added ...
Source: Comparative Clinical Pathology - January 29, 2018 Category: Pathology Source Type: research

Is vitamin D supplement accompanied with narrow band UVB effective for treatment of vitiligo?
AbstractVitiligo is an acquired autoimmune depigmenting disorder that is the result of white macules formation in the skin and mucous membranes, caused by the loss of melanocytes. It has affected 0.4 to 2.0% of world ’s population. It has been hypothesized that Vitamin D is important for skin pigmentation, but the correlation between vitamin D and vitiligo treatment response needs to be evaluated. To evaluate vitamin D supplementation effect on the vitiligo area severity index in patients treated with narrow b and UVB (NB UVB). This clinical pilot randomized study was carried out on 48 vitiligo patients who admitted ...
Source: Comparative Clinical Pathology - January 29, 2018 Category: Pathology Source Type: research

Dramatic shortening of the diagnosis of multidrug-resistant tuberculosis by the detection of rifampicin resistance using a genotypic method: GeneXpert MTB/RIF assay
AbstractIn spite of all the evolutions in medicine, tuberculosis (TB) continues to be a terrifying contagious disease. In addition, the rise of multidrug-resistant TB (MDR-TB) is considered as a thorny problem that has greatly hindered the efforts of TB control. The classical drug susceptibility testing (DST) is the reference technique, but it takes many weeks for the identification of TB and MDR-TB. On the other hand, GeneXpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF) has dramatically shortened the identification of both MTB and RIF resistance from weeks to only 2  h leading to a great breakthrough in the mo...
Source: Comparative Clinical Pathology - January 27, 2018 Category: Pathology Source Type: research

Protective potential of Tetrapleura tetraptera against trona ( kaun )-induced hepatic injury in rat models
AbstractThe antioxidant protective effect of the fruits and peels ofTetrapleura tetraptera were investigated in trona (kaun)-induced oxidative injuries in male albino rats. The peels and fruits ofT. tetraptera were air-dried and extracted with ethanol. The concentrated extracts were subjected to FTIR spectroscopy analysis to identify their chemical functional groups. Five groups of six rats each were pretreated with trona (500  mg/kg bw) for 7 days, while a sixth group was administered water only which served as normal control. Four of the pretreated groups were subjected to posttreatment withT. tetraptera extrac...
Source: Comparative Clinical Pathology - January 25, 2018 Category: Pathology Source Type: research

Mesenteric cystic lymphangioma: a case report
AbstractIn adult, cystic lymphangioma of mesentery is an extremely rare manifestation of an intraperitoneal tumor. The clinical presentations of it are variable and occasionally area incidental finding. Herein, we describe a mesenteric cystic lymphangioma in a 39-year-old Iranian woman. The patient was presented with abdominal pain in the periumbilical area from 6  h earlier in the morning. Histopathological review of the excised mass revealed large numbers of cystic lymphatic channels lined by flattened endothelial cells and had smooth muscle bundles in their walls. The patient had an uneventful postoperative period....
Source: Comparative Clinical Pathology - January 25, 2018 Category: Pathology Source Type: research

Effect of formaldehyde antimicrobial feed additive on the immune competence of chickens experimentally infected with a known Newcastle disease virus strain
AbstractThirty-seven percent (37%) formaldehyde- possessing antimicrobial activity has been used in various forms as a fumigant for mould control and as a feed additive for feed preservation. The study evaluated the effects of formaldehyde-treated poultry feed on the immune response of birds to Newcastle disease virus (NDV) infection with Kudu 113 strain. A total of 100 chicks were acquired from Zartech Hatchery, Ibadan, Nigeria. They were randomly appropriated into A, B, C and D experimental groups, with each group containing 25 birds. Group A chicks were vaccinated, fed treated feed and infected with ND virus while group...
Source: Comparative Clinical Pathology - January 24, 2018 Category: Pathology Source Type: research

Ectoparasites associated with small mammals (orders Insectivora, Eulipotyphla, and Rodentia) in Razan plain, western region of Iran
The objectives of this project were to determine the prevalence and intensity of ectoparasites of small mammals in the Razan plain region. From February 2010 to November 2013, small mammals were collected by live traps. Animals were anesthetized and ectoparasites were collected, preserved in ethanol, and then identified. A total of 192 small mammals in five species including three species of rodents, one species of shrew, and one species of hedgehog were caught of which ultimately 13 species of ectoparasites were collected: three species of sucking lice, four species of fleas, and six species of ticks. In total, 62.5% of s...
Source: Comparative Clinical Pathology - January 23, 2018 Category: Pathology Source Type: research

Protective effect of Gnetum africanum methanol leaf extract on pancreatic islet cells in rats
AbstractLaboratory rats have played a central role in studies of islet biology. Their islets have a well-defined structure with a central core of β-cells representing 60–80% of islet cells and a layer of other endocrine cells surrounding the core including α-cells, δ-cells, and pluripotent cells.Gnetum africanum is a leafy vegetable used in the management of diabetes by the people of Southern Nigeria. Despite its wide usage, its effect on alloxan-induced pancreatic cells is largely unknown. Its protective effect on pancreatic islets was studied using rats, in which diabetes was induced using alloxan ...
Source: Comparative Clinical Pathology - January 20, 2018 Category: Pathology Source Type: research

Detection of Toxoplasma gondii in infected meat samples by a latex agglutination test using anti-P30 recombinant antibody
In this study, a latex agglutination assessment for identification ofToxoplasma gondii in contaminated meat products was developed. For this propose, recombinant anti-P30 single-chain Fv (scFv) antibody gene was cloned into pET26b vector to express this antibody in high level. This gene ’s expression inEscherichia coli lead to production of significant amount of antibody (16  mg/L). After purification, the anti-P30 scFv was connected to beads of latex and used for agglutination test. These beads could agglutinate wholeToxoplasma gondii and contaminated meat samples. Thus, this rapid test may be applied to find c...
Source: Comparative Clinical Pathology - January 20, 2018 Category: Pathology Source Type: research

Correction to: Seroprevalence of orf infection based on IgM antibody detection in sheep and goats from selected small ruminant farms in Malaysia
AbstractThere were errors in the original version of this article. First is the affiliation and spelling of one of the authors, Ashwaq Ahmed Abdullah. And the other is in the affiliation 1. Corrected data are presented here. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - January 20, 2018 Category: Pathology Source Type: research

Oxidative status of growing West African dwarf goat kids fed diets containing different nutrient density
AbstractNutritional manipulation is the best option against parasitic infection in ruminants, as it has no residual effect on the animal. Nutrition improves the resilience and resistance of the infected animals and eliminates the resultant clinical anaemia. Twenty-four growing intensively raised West African dwarf (WAD) goats (10.2  + 0.78) were used to assess the effect of incremental levels of protein and energy on the performance characteristics and oxidative status of the WAD goats. The animals were individually housed and allotted to diets containing varying levels of crude protein and energy levels: hig...
Source: Comparative Clinical Pathology - January 19, 2018 Category: Pathology Source Type: research

A study of hematologic and biochemical profile in female dromedary camels during the breeding and non-breeding seasons
This study was performed to investigate hematological and serum biochemical changes during breeding and non-breeding seasons in female camels, raised in Khouzestan Province. Blood samples were collected from 32 apparently healthy female dromedary camels during December to March as breeding period and from June to October as non-breeding period. Complete blood counts and serum biochemical parameters were analyzed. The results revealed a significant rise in erythrocyte markers including RBC, Hb, HCT, and MCHC in peak breeding season. Serum creatinine concentration in the breeding season was significantly higher than the non-...
Source: Comparative Clinical Pathology - January 19, 2018 Category: Pathology Source Type: research

Clinical and pathological findings of enzootic nasal adenocarcinoma of goat
AbstractThe enzootic nasal tumor (ENT), a contagious tumor, is raised from the secretory epithelial cells of the ethmoid turbinate in animal which is caused by ENT virus type (ENTV) 2. The aim of this study is to investigate the clinical, radiological, and histopathological features of ENT or nasal adenocarcinoma in Lori-Bakhtiari breeds of goat. The affected goats showed respiratory nasal problems, continuous nasal exudate, and death resulted from progressive respiratory distress and anorexia. Dorsomedial radiographs of the skull region showed the mass in the right caudal paranasal sinus region deformation of bone in the ...
Source: Comparative Clinical Pathology - January 18, 2018 Category: Pathology Source Type: research

Effects of temperature, pH, and bile salt on antimicrobial activity of bacteriocin-like substances obtained from barley sourdough LAB
This study aimed to investigate the effects of temperature (4, 85, 121  °C), pH (2, 4, 11), and bile salt (0.3, 0.6, 1%)—as common conditions of gastrointestinal tract or thermal food processing—on antimicrobial activity of bacteriocin-like substances (BLS) obtained from barley sourdough lactic acid bacteria (LAB) against some of food-borne pathogens. In addition , the safety of LAB isolates was also evaluated. The isolates were identified by sequencing of PCR products asLactobacillus brevis,Lactobacillus curieae,Pediococcus stilesii andWeissella cibaria. At low and high temperatures and pH values and ...
Source: Comparative Clinical Pathology - January 18, 2018 Category: Pathology Source Type: research

Outbreak and management of Mycoplasma gallisepticum infection in desi chicken and turkey flocks in an organized mixed farm
AbstractIn an organized farm, desi chicken and turkeys were reported with the clinical signs of congestion of conjunctival mucous membrane, dullness, unilateral and bilateral head swelling and nasal discharge. Flock strength was 545 desi chickens and 296 turkeys of 75 and 50  days old respectively. Morbidity was 3.67 and 6.75% and mortality was 0.91 and 0.67% in chickens and turkeys respectively. On investigation, clinical signs started 12 days after the introduction of desi chicken from another organized farm. In total, 10 chickens and 20 turkeys showed clinical sign s, of which 5 desi chicken and 2 turkeys died...
Source: Comparative Clinical Pathology - January 13, 2018 Category: Pathology Source Type: research

Long-term effects of thyroxine-induced hyperthyroidism on the histological attributes of the oviduct in broiler breeder hens
AbstractA 4-week-long administration of extra thyroxine (T4) in broiler breeder hens was recommended to lower the cold-induced ascites incidence in the progeny chicks. A hypothesis was tested that the long-term-induced hyperthyroidism might influence the histological traits of oviducts in the exposed hens. A total of 70 47-week-old Cobb 500 breeder hens were randomly allotted to two treatment groups (5 replicates of 7 hens each), including the control (CON) and hyperthyroid (HYPER). Thyroxine (T4) was orally administered to the HYPER group (0.3  mg/bird day−1) for 100 successive days; whereas the CON group ...
Source: Comparative Clinical Pathology - January 6, 2018 Category: Pathology Source Type: research