The value of kidney biochemical parameters in diagnosis of acute tubular necrosis (ATN) in chickens

This study was designed to evaluate the effectiveness of biochemical factors such as blood urea nitrogen (BUN), creatinine, and serum uric acid in identifying and diagnosing acute tubular necrosis lesion in broilers. Kidney tissue and blood samples from 80 broiler chickens at the slaughter stage were collected. Tissue samples were collected in 10% formalin to detect pathological lesions. The components of BUN, creatinine, and serum uric acid were measured with commercially available kits. After preparing the histopathological sections, based on the percentage of necrosis of the renal tubules in each tissue sample, the pathological lesions were divided into 3 degrees: mild, moderate, and severe. Biochemical parameters analyzed with receiver operator characteristic (ROC) analysis to determine critical thresholds for predicting ATN. The results showed that 36 samples out of 80 samples (45%) had acute tubular necrosis (ATN) lesions; 14 (17.5%), 12 (15%), and 10 (12.5%) samples had severe, moderate, and mild tubular necrosis lesions, respectively. The amount of uric acid in chickens with moderate and severe lesions showed a significant difference between the chickens without lesions and the mild lesion. However, BUN and creatinine increased significantly only in severe ATN lesions (p <  0.05). Uric acid was the only significant predictors identified in the ROC analysis. Optimum critical thresholds for this was 5.71 mg/dl (P<0.05).  In general, the results of this study ...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research