Melatonin mitigates oxidative stress in luniron-induced testicular injury in Wistar rats

This study aims to investigate the potential protective effects of melatonin (MLT) against linuron-induced testicular toxicity and spermatogenesis damage. Rats were divided into four groups: the control group (no treatment), the MLT group that received MLT (10 mg/kg b.w), the LIN group that received LIN (120 mg/kg b.w), and (LIN/MLT) group treated with LIN and MLT. The investigated substances MLT and linuron (LIN) were given orally to the animals for 30 days. The results showed that linuron treatment-induced testicular dysfunctions demonstrated significant inhibition of p ituitary–testicular axis hormone synthesis (diminution serum levels of testosterone, FSH, and LH) associated with spermatogenesis injury improved by low Johnsen scores and increased in testis CD117 expression. Furthermore, superoxide dismutase (SOD) and catalase (CAT) activities, as well as reduce d glutathione (GSH) content, were significantly decreased. In contrast, there was a considerable rise in the activity of glutathione S-transferase (GST), glutathione peroxidase (GPx), malondialdehyde (MDA), and protein carbonyl (PCO). Our results established that oral MLT supplementation in LIN-trea ted rats restored plasma hormone levels and alleviated the adverse cytotoxic effects of LIN. According to the findings, MLT demonstrated potential as an endogenous antioxidant, effectively alleviating linuron-induced testicular oxidative injury.
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research