The therapeutic potential of saharian monovarietal virgin olive oil (olea europea l.) on hematological, histological, and antioxidant status in titanium dioxide nanoparticles-induced oxidative stress in rats

This study aimed to assess the composition of bioactive compounds and the antioxidant activity of monovarietal virgin olive oil (MVOO). Specifically, the objective was to investigate the protective effects of MVOO in alleviating the adverse effects induced by titanium dioxide nanoparticles (NPTiO2). Bioactive compounds in MVOO were analyzed using UV –visible spectrophotometry, along with High-Performance Liquid Chromatography with a Photodiode Array (HPLC–PDA) for a comprehensive assessment. Antioxidant activity in vitro was evaluated through 2,2-diphenyl-1-picrylhydrazyl (DDPH) assays and on rats in vivo. A total of twenty-four male Wistar albino rats were randomly divided into four groups: one served as the control (Control), and the three remaining groups were injected with NPTiO2. Among the NPTiO2-injected groups, one received no treatment (NPTiO2), while the other two (NPTiO2 + MVOO1 and NPTiO2 + MVOO2) were treated with different doses of MVOO (2  g/kg bw and 6 g/kg bw). Commencing 24 h after NPTiO2 injection, the treatment with MVOO began, and body weight was consistently recorded. After 6  weeks of dietary manipulation, the fasting animals were sacrificed, and evaluations of hematological parameters, biochemical parameters, and oxidative stress markers in the liver and kidney were conducted. Histological examinations were performed to confirm protective effects. The analysis reveale d significant levels of bioactive compounds in MVOO, with a remarkable...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research