Urtica dioica extract mitigates doxorubicin-induced hepatotoxicity and nephrotoxicity by suppressing oxidative stress and modulating biochemical indices: In vivo and molecular docking study

This study evaluated the hepatoprotective and nephroprotective effects of ethanol extracts ofUrtica dioica (UD)  leaves on doxorubicin-induced oxidative stress. This study comprised 5 groups: control, doxorubicin (DOX: 15 mg/kg), UD-treated group (DOX + 300 mg/kg), a second UD-treated group (DOX + 600 mg/kg), and UD-treated group (600 mg/kg alone). The in vitro antioxidant potential of UD was as sayed with FRAP and DPPH, and GCMS-identified UD phytoconstituents were docked against NADPH oxidase (2CDU) and nitric oxide synthase (2FLQ) using molecular docking tools such as Discovery Studio, Open Babel, and PyRX. UD had a concentration-dependent FRAP better than BHT and a DPPH scavenging activ ity of IC50 265.96 g/ml. The DOX group had hepatic and renal alteration that was evident in the significant (p <  0.05) elevation of hepatic (AST and ALT) and renal (BUN, creatinine, Na+) markers with reduced antioxidant markers (GPx, CAT, GSH). The CRP level of the DOX group was also significantly (p <  0.05) higher than that of the control group. The UD-treated group (DOX + 300 mg/kg) showed a significant (p <  0.05) reduction in the CRP, hepatic, and renal biomarkers, with a significant improvement in the activities of the antioxidant markers. The selected UD compounds showed good binding affinities against 2CDU and 2FLQ. Beta-Bissabolene (-9.2 and -8.0 kg/mol) and Alpha-Terpineol (-7.7 and -7.0 kg/m ol) have higher binding affinities and good ...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research