Evaluation of the effect of Dracocephalum kotschyi hydroalcoholic extract in preventing intra-abdominal adhesions after abdominal surgery in rats based on inflammatory factors (TNF- α and IL-1β)

In this study, we investigated the anti-abdominal adhesions effect of the hydroalcoholic extract ofDracocephalum kotschyi. It is an experimental study. Forty healthy male Wistar rats were divided into 6 groups and abdominal surgery was performed in all of them to induce adhesion: group 1, control (with surgery and without active ingredient); group 2, chitosan gel injection in the abdominal area before suturing the site (as anti-adhesive); group 3, administration of alcoholic extract ofDracocephalum kotschyi at a dose of 40 mg/kg; group 4, administration of alcoholic extract ofDracocephalum kotschyi at a dose of 80 mg/kg; group 5, administration of alcoholic extract ofDracocephalum kotschyi at a dose of 120 mg/kg, for a week. Then, with deep anesthesia in rats, tissue samples were taken for histological studies and determination of the amount of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) by biochemical analyses, and real-time quantitative PCR (RT-qPCR) for gene expression of tumor necrosis factor-alpha (TNF- α) and interleukin-1beta (IL-1β). The decrease in intra-abdominal adhesion index indicates the positive effect of theDracocephalum kotschyi extract, which is confirmed by these results: MDA decreased compared to the control group, SOD and CAT was increased after prescriptionDracocephalum kotschyi extract especially in DC120mg/kg. Gene expressions for two inflammatory markers were reduced in total groups compared to the control group.Dracocephalum k...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research