Emodin-induced hepatotoxicity is enhanced by 3-methylcholanthrene through activating aryl hydrocarbon receptor and inducing CYP1A1 in vitro and in vivo
CONCLUSIONS: Our results validated that the metabolism of emodin to 5-hydroxyemodin by CYP1A played an important role in the hepatocellular toxicity of emodin and provided evidence that CYP1A1 and AhR could be used to predict and validate patient-specific liver injury of PMT or other herbs containing emodin.PMID:35934134 | DOI:10.1016/j.cbi.2022.110089 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - August 7, 2022 Category: Molecular Biology Authors: Meixi Wang Zuqi Zhang Panpan Ruan Guangchen Zhang Chengrong Xiao Yuguang Wang Yue Gao Source Type: research
Rhein activated Fas-induced apoptosis pathway causing cardiotoxicity in vitro and in vivo
In this study, we found that the IC50 of rhein to H9c2 cells at 24h and 48h were 94.5 and 45.9μmol/L, respectively, with positive correlation of dose-toxicity and time-toxicity. After the treatment of rhein (106, 124 and 132μmol/L), the number of H9c2 cells decreased significantly, and the morphology of H9c2 cells showed atrophy, round shape and wall detachment. Moreover, the proportion of apoptotic cells in H9c2 cells treated with rhein was significantly increased in a dose-dependent manner. And rhein induced S phase arrest of H9c2 cells and inhibited cell proliferation. Rhein up-regulated ROS, LDH levels and low MMP bu...
Source: Toxicology Letters - May 19, 2022 Category: Toxicology Authors: Li Gang-Min Chen Jun-Ren Zhang Hui-Qiong Sun Chen Chen Guan-Ru Xiong Qiu-Yun Cao Xiao-Yu Yu Lei Lin Zi-Wei Qin Jun-Yuan Wu Liu-Jun Li Jing Pu Lin Peng Fu Xie Xiao-Fang Peng Cheng Source Type: research
Rhein activated Fas-induced apoptosis pathway causing cardiotoxicity in vitro and in vivo
In this study, we found that the IC50 of rhein to H9c2 cells at 24h and 48h were 94.5 and 45.9μmol/L, respectively, with positive correlation of dose-toxicity and time-toxicity. After the treatment of rhein (106, 124 and 132μmol/L), the number of H9c2 cells decreased significantly, and the morphology of H9c2 cells showed atrophy, round shape and wall detachment. Moreover, the proportion of apoptotic cells in H9c2 cells treated with rhein was significantly increased in a dose-dependent manner. And rhein induced S phase arrest of H9c2 cells and inhibited cell proliferation. Rhein up-regulated ROS, LDH levels and low MMP bu...
Source: Toxicology Letters - May 19, 2022 Category: Toxicology Authors: Li Gang-Min Chen Jun-Ren Zhang Hui-Qiong Sun Chen Chen Guan-Ru Xiong Qiu-Yun Cao Xiao-Yu Yu Lei Lin Zi-Wei Qin Jun-Yuan Wu Liu-Jun Li Jing Pu Lin Peng Fu Xie Xiao-Fang Peng Cheng Source Type: research
Hepatotoxicity of the Major Anthraquinones Derived From Polygoni Multiflori Radix Based on Bile Acid Homeostasis
This study aims to explore the hepatotoxic effect of PMR extract and the major PMR derived anthraquinones including emodin, chrysophanol, and physcion in mice and the underlying mechanisms based on bile acid homeostasis. After consecutively treating the ICR mice with PMR extract or individual anthraquinones for 14 or 28 days, the liver function was evaluated by measuring serum enzymes levels and liver histological examination. The compositions of bile acids (BAs) in the bile, liver, and plasma were measured by LC-MS/MS, followed by Principal Component Analysis (PCA) and Partial Least Squares Discriminate Analysis (PLS-DA)...
Source: Frontiers in Pharmacology - May 18, 2022 Category: Drugs & Pharmacology Source Type: research
Hepatic Organoid-Based High-Content Imaging Boosts Evaluation of Stereoisomerism-Dependent Hepatotoxicity of Stilbenes in Herbal Medicines
In this study, we used a hepatic organoids–based in vitro hepatotoxic evaluation system in conjunction with using high content imaging to differentiate in vivo organ hepatotoxicity of the 2,3,5,4′-tetrahydroxy-trans-stilbene-2-O-β-glucoside (trans-SG) and its cis-isomer (cis-SG). By using such an organoid platform, we successfully differentiated the two stereoisomers’ hepatotoxic potentials, which were in accordance with their differences in rodents and humans. The lesion mechanism of the toxic isomer (cis-SG) was further found as the mitochondrial injury by high-content imaging, and its hepatotoxicity could be dose...
Source: Frontiers in Pharmacology - May 17, 2022 Category: Drugs & Pharmacology Source Type: research
Transformation of Stilbene Glucosides From Reynoutria multiflora During Processing
The root of Reynoutria multiflora Thunb. Moldenke (RM, syn.: Polygonum multiflorum Thunb.) has been widely used in TCM clinical practice for centuries. The raw R. multiflora (RRM) should be processed before use, in order to reduce toxicity and increase efficiency. However, the content of trans-2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside (trans-THSG), which is considered to be the main medicinal ingredient, decreases in this process. In order to understand the changes of stilbene glycosides raw R. multiflora (RRM) and processed R. multiflora (PRM), a simple and effective method was developed by ultra high pe...
Source: Frontiers in Pharmacology - April 25, 2022 Category: Drugs & Pharmacology Source Type: research
2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside protects against neuronal cell death and traumatic brain injury-induced pathophysiology
Aging (Albany NY). 2022 Mar 21;14(undefined). doi: 10.18632/aging.203958. Online ahead of print.ABSTRACTTraumatic brain injury (TBI) is a global health issue that affects at least 10 million people per year. Neuronal cell death and brain injury after TBI, including apoptosis, inflammation, and excitotoxicity, have led to detrimental effects in TBI. 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside (THSG), a water-soluble compound extracted from the Chinese herb Polygonum multiflorum, has been shown to exert various biological functions. However, the effects of THSG on TBI is still poorly understood. THSG reduced L-glut...
Source: Aging - March 22, 2022 Category: Biomedical Science Authors: Yu-Hsin Chen Yen-Chou Chen Yu-Tang Chin Ching-Chiung Wang Ling-Ling Hwang Liang-Yo Yang Dah-Yuu Lu Source Type: research
2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside protects against neuronal cell death and traumatic brain injury-induced pathophysiology
Aging (Albany NY). 2022 Mar 21;14(undefined). doi: 10.18632/aging.203958. Online ahead of print.ABSTRACTTraumatic brain injury (TBI) is a global health issue that affects at least 10 million people per year. Neuronal cell death and brain injury after TBI, including apoptosis, inflammation, and excitotoxicity, have led to detrimental effects in TBI. 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside (THSG), a water-soluble compound extracted from the Chinese herb Polygonum multiflorum, has been shown to exert various biological functions. However, the effects of THSG on TBI is still poorly understood. THSG reduced L-glut...
Source: Aging - March 22, 2022 Category: Biomedical Science Authors: Yu-Hsin Chen Yen-Chou Chen Yu-Tang Chin Ching-Chiung Wang Ling-Ling Hwang Liang-Yo Yang Dah-Yuu Lu Source Type: research
Anti-inflammation of torachrysone-8-O- β-ᴅ-glucoside by hurdling over morphological changes of macrophages
In this study, TG significantly inhibited the Tyr-phosphorylation of focal adhesion kinase (FAK), a key regulator of morphological transformation, and downregulated FAK-mediated transcription of cytoskeleton genes. Thus, TG greatly restrained LPS-induced morphological transformation of macrophage cells into M1 type and reduced their adhesion. The inhibition of TG on FAK phosphorylation also blocked the binding between phosphor-FAK and pyruvate kinase (PK), which contributed to the inhibition of PK activity and limited the high glycolysis rate of M1 metabolic phenotype. Moreover, TG ameliorated defective function of the TCA...
Source: International Immunopharmacology - January 29, 2022 Category: Allergy & Immunology Authors: Wenlong Zhao Di Xu Lin Zhang Huihui Meng Qi Zheng Junsong Wang Source Type: research
Simultaneously screening multiple UGT1A1 inhibitors from Polygonum multiflorum root using ultrafiltration liquid chromatography-mass spectrometry
This study was deliberately designed to simultaneously screen the UGT1A1 inhibitors from PMR and their co-contribution to the hepatotoxicity was evaluated. With ultrafiltration coupled to liquid chromatography-mass spectrometry method, four compounds namely cis-2,3,5,4'-tetrahydroxystilbene-2-O-β-glucoside, trans-2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, emodin-8-O-β-D-glucoside and emodin were screened out, displaying the in vitro inhibitory activities against UGT1A1 with IC50 values of 76.23, 18.70, 62.18, 34.02 μM, respectively. The varying activities of screened UGT1A1 inhibitors were explained by performing...
Source: Biomedical Chromatography : BMC - December 18, 2021 Category: Biomedical Science Authors: Yan Jiang Cai Zhang Xian Zheng Zhen Zhao Hui-Jun Li Source Type: research
Simultaneously screening multiple UGT1A1 inhibitors from Polygonum multiflorum root using ultrafiltration liquid chromatography-mass spectrometry
This study was deliberately designed to simultaneously screen the UGT1A1 inhibitors from PMR and their co-contribution to the hepatotoxicity was evaluated. With ultrafiltration coupled to liquid chromatography-mass spectrometry method, four compounds namely cis-2,3,5,4'-tetrahydroxystilbene-2-O-β-glucoside, trans-2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, emodin-8-O-β-D-glucoside and emodin were screened out, displaying the in vitro inhibitory activities against UGT1A1 with IC50 values of 76.23, 18.70, 62.18, 34.02 μM, respectively. The varying activities of screened UGT1A1 inhibitors were explained by performing...
Source: Biomedical Chromatography : BMC - December 18, 2021 Category: Biomedical Science Authors: Yan Jiang Cai Zhang Xian Zheng Zhen Zhao Hui-Jun Li Source Type: research
Biomarkers and Mechanism Analysis for Polygoni Multiflori Radix Preparata-Induced Liver Injury by UHPLC-Q-TOF-MS-Based Metabolomics
CONCLUSIONS: This study is contributed to understand the potential pathogenesis of PMP-induced liver injury. The metabonomic method may be a valuable tool for the clinical diagnosis of PMP-induced liver injury.PMID:34858511 | PMC:PMC8632464 | DOI:10.1155/2021/7677392 (Source: Evidence-based Complementary and Alternative Medicine)
Source: Evidence-based Complementary and Alternative Medicine - December 3, 2021 Category: Complementary Medicine Authors: Liming Wang Zhida Wang Yanchao Xing Erwei Liu Xiumei Gao Linlin Wang Zhifei Fu Source Type: research
Effect of Observation of Shou Hui Tong Bian Capsule (Polygonum Multiflorum and Aloe-Based Herbal Capsule for Cathartic Effect) in Rapid Rehabilitation of Joint Surgery
CONCLUSION: The curative effect of Shou Hui Tong Bian capsule on patients undergoing arthroscopic joint surgery and joint replacement during perioperative period is obviously superior to that of conventional treatment. It can effectively improve the total effective rate, shorten the first exhaust time, and increase the number of patients without abdominal distension after treatment. It was safe and effective, and worthy of clinical promotion.PMID:34707664 | PMC:PMC8545539 | DOI:10.1155/2021/2268464 (Source: Evidence-based Complementary and Alternative Medicine)
Source: Evidence-based Complementary and Alternative Medicine - October 28, 2021 Category: Complementary Medicine Authors: Shu Huang Yong Xie Zhiyan Huang Guimin Zhang Guanyu Chen Jun Yuan Jing Wang Liu Xiangyang Yizhao Zhou Source Type: research
