Knowledge-attitude and practice of Anthrax and brucellosis: Implications for zoonotic disease surveillance and control in pastoral communities of Afar and Somali region, Ethiopia
ConclusionOur study revealed significant under-reporting of Anthrax and brucellosis, and weak prevention and response in humans, mostly associated with poor disease knowledge of healthcare providers. Ability to respond to animal outbreaks was limited by vaccine and drugs availability, timely vaccine administration and the mobility of pastoralists. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - April 4, 2024 Category: Tropical Medicine Authors: Rea Tschopp Source Type: research
A porcine kidney-derived clonal cell line with clear genetic annotation is highly susceptible to African swine fever virus
AbstractAfrican Swine Fever virus (ASFV), the causative agent of African swine fever, is a highly lethal hemorrhagic virus affecting domestic pigs and wild boars. The primary target cells for ASFV infection are porcine alveolar macrophages (PAMs), which are difficult to obtain and maintain in vitro, and less subjective to genetic editing. To overcome these issues and facilitate ASFV research, we obtained a subclonal cell line PK1-C5 by subcloning LLC-PK1 cells that support stable ASFV proliferation. This consequential cell line exhibited high ASFV infection levels and similar viral growth characteristics to PAMs, while als...
Source: Veterinary Research - April 4, 2024 Category: Veterinary Research Source Type: research
GSE262852 Neoantigen-Augmented iPSC-based Cancer Vaccine Combined with Local Radiotherapy Promotes Systemic Antitumor Immunity in Poorly Immunogenic Cancers
Contributors : Kevin C Huang ; William T Chen ; Jia-Yi Chen ; Chien-Yueh Lee ; Chia-Hsin Wu ; Chia-Ying Lai ; Pei-Chen Yang ; Ji-An Liang ; An ‑Cheng Shiau ; K S Chao ; Tao-Wei KeSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusAlthough irradiated induced-pluripotent stem cells (iPSCs) as a prophylactic cancer vaccine elicit an antitumor immune response, the therapeutic efficacy of iPSC-based cancer vaccines is not promising due to their insufficient antigenicity and the immunosuppressive tumor microenvironment. Here, we found that neoantigen-engineered iPSC cancer vaccines c...
Source: GEO: Gene Expression Omnibus - April 3, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Whole genome sequencing of recombinant viruses obtained from co-infection and superinfection of Vero cells with modified vaccinia virus ankara vectored influenza vaccine and a naturally occurring cowpox virus
In this study, we selected the putative recombinant viruses and performed genomic characterization of these viruses. Some putative recombinant viruses displayed plaque morphology distinct of that of the parental viruses. Our analysis demonstrated that they had mosaic genomes of different lengths. The recombinant viruses, with a genome more similar to MVA-HANP (>50%), rescued deleted and/or fragmented genes in MVA and gained new host ranges genes. Our analysis also revealed that some MVA-HANP contained a partially deleted transgene expression cassette and one recombinant virus contained part of the transgene expression c...
Source: Frontiers in Immunology - April 3, 2024 Category: Allergy & Immunology Source Type: research
Viruses, Vol. 16, Pages 559: Measles Virus-Based Vaccine Expressing Membrane-Anchored Spike of SARS-CoV-2 Inducing Efficacious Systemic and Mucosal Humoral Immunity in Hamsters
ao-Qi Chen
Jia Lu
Shuo Shen
As SARS-CoV-2 continues to evolve and COVID-19 cases rapidly increase among children and adults, there is an urgent need for a safe and effective vaccine that can elicit systemic and mucosal humoral immunity to limit the emergence of new variants. Using the Chinese Hu191 measles virus (MeV-hu191) vaccine strain as a backbone, we developed MeV chimeras stably expressing the prefusion forms of either membrane-anchored, full-length spike (rMeV-preFS), or its soluble secreted spike trimers with the help of the SP-D trimerization tag (rMeV-S+SPD) of SARS-CoV-2 Omicron BA.2. The two vaccine ca...
Source: Viruses - April 3, 2024 Category: Virology Authors: Zhi-Hui Yang Yan-Li Song Jie Pei Song-Zhuang Li Rui-Lun Liu Yu Xiong Jie Wu Yuan-Lang Liu Hui-Fen Fan Jia-Hui Wu Ze-Jun Wang Jing Guo Sheng-Li Meng Xiao-Qi Chen Jia Lu Shuo Shen Tags: Article Source Type: research
Intranasal administration of unadjuvanted SARS-CoV-2 spike antigen boosts antigen-specific immune responses induced by parenteral protein subunit vaccine prime in mice and hamsters
Eur J Immunol. 2024 Apr 1:e2350620. doi: 10.1002/eji.202350620. Online ahead of print.ABSTRACTWith the continued transmission of SARS-CoV-2 across widely vaccinated populations, it remains important to develop new vaccines and vaccination strategies capable of providing protective immunity and limiting the spread of disease. Heterologous prime-boost vaccination based on the selection of different vaccine formulations and administration routes for priming and booster doses presents a promising strategy for inducing broader immune responses in key systemic and respiratory mucosal compartments. Intranasal vaccination can indu...
Source: European Journal of Immunology - April 2, 2024 Category: Allergy & Immunology Authors: Gerard Agbayani Bassel Akache Tyler M Renner Anh Tran Matthew Stuible Renu Dudani Blair A Harrison Diana Duque Jegarubee Bavananthasivam Lise Deschatelets Usha D Hemraz Sophie R égnier Yves Durocher Michael J McCluskie Source Type: research
Development, Strategies, and Challenges for Tularemia Vaccine
This study will investigate some possible vaccines and the challenges they face during development.PMID:38564047 | DOI:10.1007/s00284-024-03658-0 (Source: Current Microbiology)
Source: Current Microbiology - April 2, 2024 Category: Microbiology Authors: Safoura Moradkasani Max Maurin Amir Salek Farrokhi Saber Esmaeili Source Type: research
Intranasal administration of unadjuvanted SARS-CoV-2 spike antigen boosts antigen-specific immune responses induced by parenteral protein subunit vaccine prime in mice and hamsters
Eur J Immunol. 2024 Apr 1:e2350620. doi: 10.1002/eji.202350620. Online ahead of print.ABSTRACTWith the continued transmission of SARS-CoV-2 across widely vaccinated populations, it remains important to develop new vaccines and vaccination strategies capable of providing protective immunity and limiting the spread of disease. Heterologous prime-boost vaccination based on the selection of different vaccine formulations and administration routes for priming and booster doses presents a promising strategy for inducing broader immune responses in key systemic and respiratory mucosal compartments. Intranasal vaccination can indu...
Source: European Journal of Immunology - April 2, 2024 Category: Allergy & Immunology Authors: Gerard Agbayani Bassel Akache Tyler M Renner Anh Tran Matthew Stuible Renu Dudani Blair A Harrison Diana Duque Jegarubee Bavananthasivam Lise Deschatelets Usha D Hemraz Sophie R égnier Yves Durocher Michael J McCluskie Source Type: research
Perceptions, attitudes, and willingness of healthcare and frontline workers to participate in an Ebola vaccine trial in Uganda
CONCLUSIONS: Overall, information sharing leads to a better understanding and acceptance of vaccine trials and a positive vaccination experience can be a deciding factor in the acceptance of others. Particular attention should be paid to involving the community in addressing misconceptions and fears, while ensuring that participants have access to vaccination sites in terms of transport, and that they are properly accommodated at the study site including staying for a reasonable period of time.PMID:38565464 | DOI:10.1016/j.vaccine.2024.03.053 (Source: Vaccine)
Source: Vaccine - April 2, 2024 Category: Allergy & Immunology Authors: Geofrey Kimbugwe Renaud Vatrinet Juliet A Mwanga Ronald Kakuru Doreen Mpeirwe Suzan Logoose Kimton Opio Mateus Kambale Janet Seeley Rebecca F Grais Caroline Marquer Pontiano Kaleebu Agnes Ssali Source Type: research
Development, Strategies, and Challenges for Tularemia Vaccine
This study will investigate some possible vaccines and the challenges they face during development.PMID:38564047 | DOI:10.1007/s00284-024-03658-0 (Source: Current Microbiology)
Source: Current Microbiology - April 2, 2024 Category: Microbiology Authors: Safoura Moradkasani Max Maurin Amir Salek Farrokhi Saber Esmaeili Source Type: research
Perceptions, attitudes, and willingness of healthcare and frontline workers to participate in an Ebola vaccine trial in Uganda
CONCLUSIONS: Overall, information sharing leads to a better understanding and acceptance of vaccine trials and a positive vaccination experience can be a deciding factor in the acceptance of others. Particular attention should be paid to involving the community in addressing misconceptions and fears, while ensuring that participants have access to vaccination sites in terms of transport, and that they are properly accommodated at the study site including staying for a reasonable period of time.PMID:38565464 | DOI:10.1016/j.vaccine.2024.03.053 (Source: Vaccine)
Source: Vaccine - April 2, 2024 Category: Allergy & Immunology Authors: Geofrey Kimbugwe Renaud Vatrinet Juliet A Mwanga Ronald Kakuru Doreen Mpeirwe Suzan Logoose Kimton Opio Mateus Kambale Janet Seeley Rebecca F Grais Caroline Marquer Pontiano Kaleebu Agnes Ssali Source Type: research
Development, Strategies, and Challenges for Tularemia Vaccine
This study will investigate some possible vaccines and the challenges they face during development.PMID:38564047 | DOI:10.1007/s00284-024-03658-0 (Source: Current Microbiology)
Source: Current Microbiology - April 2, 2024 Category: Microbiology Authors: Safoura Moradkasani Max Maurin Amir Salek Farrokhi Saber Esmaeili Source Type: research
Experimental trials of predicted CD4+ and CD8+ T-cell epitopes of respiratory syncytial virus
ConclusionIn conclusion, epitopes of the F protein showed considerable immune response and are suitable for further validation. (Source: Frontiers in Immunology)
Source: Frontiers in Immunology - April 2, 2024 Category: Allergy & Immunology Source Type: research
False positive findings associated with adenoviral vector-based vaccine underscore the regulatory necessity to eliminate abnormal toxicity test
In conclusion, ATT, which requires unnecessary animal usage and competes for resources which otherwise can be spent on innovative medicine research, should be deleted permanently as batch release test by regulatory authorities around the world.PMID:38561146 | DOI:10.1016/j.yrtph.2024.105617 (Source: Regulatory Toxicology and Pharmacology : RTP)
Source: Regulatory Toxicology and Pharmacology : RTP - April 1, 2024 Category: Toxicology Authors: Ryan Meng Mark van Ooij Yali Li Yunhai Zhang Jianxun Xie Source Type: research
False positive findings associated with adenoviral vector-based vaccine underscore the regulatory necessity to eliminate abnormal toxicity test
In conclusion, ATT, which requires unnecessary animal usage and competes for resources which otherwise can be spent on innovative medicine research, should be deleted permanently as batch release test by regulatory authorities around the world.PMID:38561146 | DOI:10.1016/j.yrtph.2024.105617 (Source: Regulatory Toxicology and Pharmacology : RTP)
Source: Regulatory Toxicology and Pharmacology : RTP - April 1, 2024 Category: Toxicology Authors: Ryan Meng Mark van Ooij Yali Li Yunhai Zhang Jianxun Xie Source Type: research
