In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay
In this study, we designed a chemiluminescence dot blot assay to detect the 3A and 3B NSPs of FMDV. It is sensitive enough to detect up to 20 ng of the NSP, and exhibited specificity as it does not react with the viral SPs. This cost-effective assay holds promise in quality control assessment in FMD vaccine manufacturing.PMID:38479084 | DOI:10.1016/j.jviromet.2024.114906 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Uzma Jabeen Kailash Singh Bisht Huildore Bommanna Ranjitha Madhusudan Hosamani Beeragere Parameshwaraiah Sreenivasa Pratik M Kulkarni Dombesara Chandrashekar Nidhi Rajegowdanadoddi Lakshmana Amulya Veerakyathappa Bhanuprakash Hosur Joyappa Dechamma Aniket Source Type: research
Maintaining the quality of vaccines through the use of standards: Current challenges and future opportunities
Biologicals. 2024 Mar 12;86:101756. doi: 10.1016/j.biologicals.2024.101756. Online ahead of print.ABSTRACTAn international hybrid meeting held 21-22 June 2023 in Ottawa, Canada brought together regulators, scientists, and industry experts to discuss a set of principles and best practices in the development and implementation of standards. Although the use of international standards (ISs) and international units (IUs) has been an essential part of ensuring human and animal vaccine quality in the past decades, the types and uses of standards have expanded with technological advances in manufacture and testing of vaccines. Th...
Source: Biologicals : Journal of the International Association of Biological Standardization - March 13, 2024 Category: Biotechnology Authors: Nancy C Sajjadi Ryan Brady Carmen Jungb äck Laurent Mallet Catherine Milne Sandra Prior Gayle Pulle Peter Rigsby Tim Schofield Richard Siggers Dean Smith Paul Stickings Sylvie Uhlrich Wim Van Molle Angela Walker Tong Wu Tiequn Zhou Maria Baca-Estrada Source Type: research
Alum/CpG adjuvant promotes immunogenicity of inactivated SARS-CoV-2 Omicron vaccine through enhanced humoral and cellular immunity
Virology. 2024 Mar 7;594:110050. doi: 10.1016/j.virol.2024.110050. Online ahead of print.ABSTRACTThe SARS-CoV-2 Omicron variant, which was classified as a variant of concern (VOC) by the World Health Organization on 26 November 2021, has attracted worldwide attention for its high transmissibility and immune evasion ability. The existing COVID-19 vaccine has been shown to be less effective in preventing Omicron variant infection and symptomatic infection, which brings new challenges to vaccine development and application. Here, we evaluated the immunogenicity and safety of an Omicron variant COVID-19 inactivated vaccine con...
Source: Virology - March 13, 2024 Category: Virology Authors: Xiaotong Zheng Rong Yang Yuxiu Zhao Yadan Zhang Guangying Yuan Weidong Li Zhuangzhuang Xiao Xiaofei Dong Meng Ma Yancen Guo Wei Wang Xue Zhao Hongqiang Yang Shaoting Qiu Zheng Peng Ankang Liu Shouzhi Yu Yuntao Zhang Source Type: research
In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay
In this study, we designed a chemiluminescence dot blot assay to detect the 3A and 3B NSPs of FMDV. It is sensitive enough to detect up to 20 ng of the NSP, and exhibited specificity as it does not react with the viral SPs. This cost-effective assay holds promise in quality control assessment in FMD vaccine manufacturing.PMID:38479084 | DOI:10.1016/j.jviromet.2024.114906 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Uzma Jabeen Kailash Singh Bisht Huildore Bommanna Ranjitha Madhusudan Hosamani Beeragere Parameshwaraiah Sreenivasa Pratik M Kulkarni Dombesara Chandrashekar Nidhi Rajegowdanadoddi Lakshmana Amulya Veerakyathappa Bhanuprakash Hosur Joyappa Dechamma Aniket Source Type: research
Maintaining the quality of vaccines through the use of standards: Current challenges and future opportunities
Biologicals. 2024 Mar 12;86:101756. doi: 10.1016/j.biologicals.2024.101756. Online ahead of print.ABSTRACTAn international hybrid meeting held 21-22 June 2023 in Ottawa, Canada brought together regulators, scientists, and industry experts to discuss a set of principles and best practices in the development and implementation of standards. Although the use of international standards (ISs) and international units (IUs) has been an essential part of ensuring human and animal vaccine quality in the past decades, the types and uses of standards have expanded with technological advances in manufacture and testing of vaccines. Th...
Source: Biologicals : Journal of the International Association of Biological Standardization - March 13, 2024 Category: Biotechnology Authors: Nancy C Sajjadi Ryan Brady Carmen Jungb äck Laurent Mallet Catherine Milne Sandra Prior Gayle Pulle Peter Rigsby Tim Schofield Richard Siggers Dean Smith Paul Stickings Sylvie Uhlrich Wim Van Molle Angela Walker Tong Wu Tiequn Zhou Maria Baca-Estrada Source Type: research
Alum/CpG adjuvant promotes immunogenicity of inactivated SARS-CoV-2 Omicron vaccine through enhanced humoral and cellular immunity
Virology. 2024 Mar 7;594:110050. doi: 10.1016/j.virol.2024.110050. Online ahead of print.ABSTRACTThe SARS-CoV-2 Omicron variant, which was classified as a variant of concern (VOC) by the World Health Organization on 26 November 2021, has attracted worldwide attention for its high transmissibility and immune evasion ability. The existing COVID-19 vaccine has been shown to be less effective in preventing Omicron variant infection and symptomatic infection, which brings new challenges to vaccine development and application. Here, we evaluated the immunogenicity and safety of an Omicron variant COVID-19 inactivated vaccine con...
Source: Virology - March 13, 2024 Category: Virology Authors: Xiaotong Zheng Rong Yang Yuxiu Zhao Yadan Zhang Guangying Yuan Weidong Li Zhuangzhuang Xiao Xiaofei Dong Meng Ma Yancen Guo Wei Wang Xue Zhao Hongqiang Yang Shaoting Qiu Zheng Peng Ankang Liu Shouzhi Yu Yuntao Zhang Source Type: research
In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay
In this study, we designed a chemiluminescence dot blot assay to detect the 3A and 3B NSPs of FMDV. It is sensitive enough to detect up to 20 ng of the NSP, and exhibited specificity as it does not react with the viral SPs. This cost-effective assay holds promise in quality control assessment in FMD vaccine manufacturing.PMID:38479084 | DOI:10.1016/j.jviromet.2024.114906 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Uzma Jabeen Kailash Singh Bisht Huildore Bommanna Ranjitha Madhusudan Hosamani Beeragere Parameshwaraiah Sreenivasa Pratik M Kulkarni Dombesara Chandrashekar Nidhi Rajegowdanadoddi Lakshmana Amulya Veerakyathappa Bhanuprakash Hosur Joyappa Dechamma Aniket Source Type: research
Maintaining the quality of vaccines through the use of standards: Current challenges and future opportunities
Biologicals. 2024 Mar 12;86:101756. doi: 10.1016/j.biologicals.2024.101756. Online ahead of print.ABSTRACTAn international hybrid meeting held 21-22 June 2023 in Ottawa, Canada brought together regulators, scientists, and industry experts to discuss a set of principles and best practices in the development and implementation of standards. Although the use of international standards (ISs) and international units (IUs) has been an essential part of ensuring human and animal vaccine quality in the past decades, the types and uses of standards have expanded with technological advances in manufacture and testing of vaccines. Th...
Source: Biologicals : Journal of the International Association of Biological Standardization - March 13, 2024 Category: Biotechnology Authors: Nancy C Sajjadi Ryan Brady Carmen Jungb äck Laurent Mallet Catherine Milne Sandra Prior Gayle Pulle Peter Rigsby Tim Schofield Richard Siggers Dean Smith Paul Stickings Sylvie Uhlrich Wim Van Molle Angela Walker Tong Wu Tiequn Zhou Maria Baca-Estrada Source Type: research
In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay
In this study, we designed a chemiluminescence dot blot assay to detect the 3A and 3B NSPs of FMDV. It is sensitive enough to detect up to 20 ng of the NSP, and exhibited specificity as it does not react with the viral SPs. This cost-effective assay holds promise in quality control assessment in FMD vaccine manufacturing.PMID:38479084 | DOI:10.1016/j.jviromet.2024.114906 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Uzma Jabeen Kailash Singh Bisht Huildore Bommanna Ranjitha Madhusudan Hosamani Beeragere Parameshwaraiah Sreenivasa Pratik M Kulkarni Dombesara Chandrashekar Nidhi Rajegowdanadoddi Lakshmana Amulya Veerakyathappa Bhanuprakash Hosur Joyappa Dechamma Aniket Source Type: research
In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay
In this study, we designed a chemiluminescence dot blot assay to detect the 3A and 3B NSPs of FMDV. It is sensitive enough to detect up to 20 ng of the NSP, and exhibited specificity as it does not react with the viral SPs. This cost-effective assay holds promise in quality control assessment in FMD vaccine manufacturing.PMID:38479084 | DOI:10.1016/j.jviromet.2024.114906 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Uzma Jabeen Kailash Singh Bisht Huildore Bommanna Ranjitha Madhusudan Hosamani Beeragere Parameshwaraiah Sreenivasa Pratik M Kulkarni Dombesara Chandrashekar Nidhi Rajegowdanadoddi Lakshmana Amulya Veerakyathappa Bhanuprakash Hosur Joyappa Dechamma Aniket Source Type: research
Viruses, Vol. 16, Pages 441: Evaluation of Vaccine Immunogenicity & mdash;Correlates to Real-World Protection: Influenza
Viruses, Vol. 16, Pages 441: Evaluation of Vaccine Immunogenicity—Correlates to Real-World Protection: Influenza
Viruses doi: 10.3390/v16030441
Authors:
Csaba Laszlofy
Gyorgy Fazekas
Zoltan Barath
Zoltan Vajo
Recent events highlighted that, despite decades of studying vaccine immunogenicity and efforts toward finding correlates of protection, evaluating real-world vaccine efficacy as well as establishing meaningful licensing criteria still represents a significant challenge. In this paper, we review all aspects of influenza vaccine immunogenicity, including animal and human challenge studies, humora...
Source: Viruses - March 12, 2024 Category: Virology Authors: Csaba Laszlofy Gyorgy Fazekas Zoltan Barath Zoltan Vajo Tags: Review Source Type: research
Molecular characterization of the < em > meq < /em > oncogene of Marek's disease virus in vaccinated Brazilian poultry farms reveals selective pressure on prevalent strains
This study sheds light on the circulation of MDV strains capable of infecting vaccinated birds. We emphasize the urgency of adopting preventive measures to manage MDV outbreaks threatening the poultry farming industry.PMID:38465827 | DOI:10.1080/01652176.2024.2318198 (Source: Veterinary Quarterly)
Source: Veterinary Quarterly - March 11, 2024 Category: Veterinary Research Authors: Ruy D Chac ón Christian J S ánchez-Llatas Sarah L Pajuelo Andrea J Diaz Forero Victor Jimenez-Vasquez Jack A M édico Luis F Soto-Ugaldi Claudete S Astolfi-Ferreira Antonio J Piantino Ferreira Source Type: research
Role of vaccination in patients with human monkeypox virus and its cardiovascular manifestations
Ann Med Surg (Lond). 2024 Jan 4;86(3):1506-1516. doi: 10.1097/MS9.0000000000001674. eCollection 2024 Mar.ABSTRACTHuman monkeypox, caused by the monkeypox virus (MPXV), is an emerging infectious disease with the potential for human-to-human transmission and diverse clinical presentations. While generally considered milder than smallpox, it can lead to severe cardiovascular complications. The virus primarily spreads through contact with infected animals or through human-to-human transmission. Cardiovascular involvement in human monkeypox is rare but has been associated with myocarditis, pericarditis, arrhythmias, and even fu...
Source: Annals of Medicine - March 11, 2024 Category: Internal Medicine Authors: Khawaja Usama Maqbool Muhammad Talha Akhtar Shayan Ayub Fnu Simran Jahanzeb Malik Maria Malik Rafia Zubair Amin Mehmoodi Source Type: research
Glycolytic enzymes as vaccines against schistosomiasis: testing schistosoma mansoni phosphoglycerate mutase in mice
J Parasitol. 2024 Mar 1;110(2):96-105. doi: 10.1645/23-7.ABSTRACTSchistosomiasis is a globally burdensome parasitic disease caused by flatworms (blood flukes) in the genus Schistosoma. The current standard treatment for schistosomiasis is the drug praziquantel, but there is an urgent need to advance novel interventions such as vaccines. Several glycolytic enzymes have been evaluated as vaccine targets for schistosomiasis, and data from these studies are reviewed here. Although these parasites are canonically considered to be intracellular, proteomic analysis has revealed that many schistosome glycolytic enzymes are additio...
Source: The Journal of Parasitology - March 11, 2024 Category: Parasitology Authors: David B Pirovich Akram A Da'dara Patrick J Skelly Source Type: research
Molecular characterization of the < em > meq < /em > oncogene of Marek's disease virus in vaccinated Brazilian poultry farms reveals selective pressure on prevalent strains
This study sheds light on the circulation of MDV strains capable of infecting vaccinated birds. We emphasize the urgency of adopting preventive measures to manage MDV outbreaks threatening the poultry farming industry.PMID:38465827 | PMC:PMC10930104 | DOI:10.1080/01652176.2024.2318198 (Source: Veterinary Quarterly)
Source: Veterinary Quarterly - March 11, 2024 Category: Veterinary Research Authors: Ruy D Chac ón Christian J S ánchez-Llatas Sarah L Pajuelo Andrea J Diaz Forero Victor Jimenez-Vasquez Jack A M édico Luis F Soto-Ugaldi Claudete S Astolfi-Ferreira Antonio J Piantino Ferreira Source Type: research
