Cisd2 deficiency impairs neutrophil function by regulating calcium homeostasis via Calnexin and SERCA
BMB Rep. 2024 Apr 17:6179. Online ahead of print.ABSTRACTIn the context of aging, the susceptibility to infectious diseases increases, leading to heightened morbidity and mortality. This phenomenon, termed immunosenescence, is characterized by dysregulation in the aging immune system, including abnormal alterations in lymphocyte composition, elevated basal inflammation, and the accumulation of senescent T cells. Such changes contribute to increased autoimmune diseases, enhanced infection severity, and reduced responsiveness to vaccines. Utilizing aging animal models becomes imperative for a comprehensive understanding of i...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Un Yung Choi Youn Jung Choi Shin-Ae Lee Ji-Seung Yoo Source Type: research
Effect of different levels of single cell protein and probiotic microorganisms on performance, immunological responses, and intestinal histology in laying hens
AbstractAn experiment was conducted to evaluate the effects of single-cell protein (SCP), as a valuable by-product and substitute for soybean meal, and multistrain probiotics on the performance, blood biochemistry, intestinal histomorphology, ileal microbiota, and immune responses in laying hens. Leghorn laying hens were randomly assigned to nine experimental treatments with eight replicates of five birds each. Dietary treatments consisted of three levels of replacement of soybean meal with SCP (0.0%, 30%, and 60%) and three levels of probiotics (0.0, 50, 100 mg/kg) offered through 70 days of main recording. The result ...
Source: Journal of Animal Physiology and Animal Nutrition - April 17, 2024 Category: Zoology Authors: Rasoul Rashedi Dehsahraee,
Amir Hossein Mahdavi,
Mohammad Sedghi,
Hassan Saleh Tags: ORIGINAL ARTICLE Source Type: research
Flock level socio-economic and other associated risk factors for Peste des petits ruminants (PPR) exposure in sheep and goats in Madhya Pradesh state, India
AbstractTo effectively control and eradicate PPR, the comprehensive understanding of risk factors associated with PPR exposure is vital. Hence, this study investigated socioeconomic and other associated risk determinants for PPR exposure at flock level in sheep and goats in a non-vaccination programme implemented Madhya Pradesh state India. A total of 410 sheep and goat flocks, comprised mostly of goats but also some mixed flocks, were surveyed during 2016 using a multistage random sampling procedure. Further, 230 blood samples were also collected from the farmers-reported PPR affected flocks and sera were tested using c-E...
Source: Tropical Animal Health and Production - April 16, 2024 Category: Veterinary Research Source Type: research
Development of a novel monoclonal antibody-based competitive ELISA for antibody detection against bovine leukemia virus
In conclusion, the novel p24 cELISA exhibited the potential to be a reliable and efficient diagnostic tool for BLV serological detection with a broad application prospect.PMID:38621561 | DOI:10.1016/j.ijbiomac.2024.131446 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - April 15, 2024 Category: Biochemistry Authors: Jing Wang Chao Sun Zhe Hu Fang Wang Jitao Chang Ming Gao Dandan Ye Qi Jia Hui Zou Luc Willems Zhigang Jiang Xin Yin Source Type: research
Cutting Edge: First Lung Infection Permanently Enlarges Lymph Nodes and Enhances New T Cell Responses
J Immunol. 2024 Apr 15:ji2400010. doi: 10.4049/jimmunol.2400010. Online ahead of print.ABSTRACTHumans experience frequent respiratory infections. Immunology and vaccinology studies in mice are typically performed in naive specific pathogen-free animals responding to their very first respiratory challenge. We found that the first respiratory infection induces lifelong enlargement of the lung-draining mediastinal lymph nodes (medLNs). Furthermore, infection-experienced medLNs supported better naive T cell surveillance and effector responses to new unrelated infections that exhibited more biased accumulation and memory establ...
Source: Journal of Immunology - April 15, 2024 Category: Allergy & Immunology Authors: J Michael Stolley Milcah C Scott Stephen D O'Flanagan Marco K ünzli Courtney A Matson Eyob Weyu Ryan A Langlois Vaiva Vezys David Masopust Source Type: research
Development of a novel monoclonal antibody-based competitive ELISA for antibody detection against bovine leukemia virus
In conclusion, the novel p24 cELISA exhibited the potential to be a reliable and efficient diagnostic tool for BLV serological detection with a broad application prospect.PMID:38621561 | DOI:10.1016/j.ijbiomac.2024.131446 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - April 15, 2024 Category: Biochemistry Authors: Jing Wang Chao Sun Zhe Hu Fang Wang Jitao Chang Ming Gao Dandan Ye Qi Jia Hui Zou Luc Willems Zhigang Jiang Xin Yin Source Type: research
Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus in Xinjiang, China, from 2020 to 2022
In this study, we investigated the genetic characteristics of currently prevalent porcine epidemic diarrhea virus (PEDV) strains in the region. We collected 548 samples from animals with suspected PED on large-scale pig farms in Xinjiang. Of these, 258 tested positive for PEDV by RT-PCR, yielding an overall positivity rate of 47.08%.S1 gene sequencing and phylogenetic analysis were conducted on 23 randomly selected RT-PCR-positive samples. Three endemic strains of PEDV (PEDV/CH/XU/2020, PEDV/CH/XK/2020, and PEDV/CH/XA/2020) were isolated, and their complete genome sequences were analyzed for evidence of genetic recombinati...
Source: Archives of Virology - April 15, 2024 Category: Virology Source Type: research
Lactiplantibacillus plantarum surface-displayed VP6 (PoRV) protein can prevent PoRV infection in piglets
Int Immunopharmacol. 2024 Apr 13;133:112079. doi: 10.1016/j.intimp.2024.112079. Online ahead of print.ABSTRACTPorcine rotavirus (PoRV) poses a threat to the development of animal husbandry and human health, leading to substantial economic losses. VP6 protein is the most abundant component in virus particles and also the core structural protein of the virus. Firstly, this study developed an antibiotic-resistance-free, environmentally friendly expression vector, named asd-araC-PBAD-alr (AAPA). Then Recombinant Lactiplantibacillus plantarum (L. plantarum) strains induced by arabinose to express VP6 and VP6-pFc fusion proteins...
Source: International Immunopharmacology - April 14, 2024 Category: Allergy & Immunology Authors: Junhong Wang Haixu Wang Dongliang Zhang Fangyuan Liu Xiaoxu Li Ming Gao Mingyang Cheng Hongyu Bao Jiaxing Zhan Yan Zeng Chunfeng Wang Xin Cao Source Type: research
Lactiplantibacillus plantarum surface-displayed VP6 (PoRV) protein can prevent PoRV infection in piglets
Int Immunopharmacol. 2024 Apr 13;133:112079. doi: 10.1016/j.intimp.2024.112079. Online ahead of print.ABSTRACTPorcine rotavirus (PoRV) poses a threat to the development of animal husbandry and human health, leading to substantial economic losses. VP6 protein is the most abundant component in virus particles and also the core structural protein of the virus. Firstly, this study developed an antibiotic-resistance-free, environmentally friendly expression vector, named asd-araC-PBAD-alr (AAPA). Then Recombinant Lactiplantibacillus plantarum (L. plantarum) strains induced by arabinose to express VP6 and VP6-pFc fusion proteins...
Source: International Immunopharmacology - April 14, 2024 Category: Allergy & Immunology Authors: Junhong Wang Haixu Wang Dongliang Zhang Fangyuan Liu Xiaoxu Li Ming Gao Mingyang Cheng Hongyu Bao Jiaxing Zhan Yan Zeng Chunfeng Wang Xin Cao Source Type: research
Genotoxicity and safety pharmacology of the rVSVInd(GML)-mspSGtc vaccine against SARS-CoV-2 in Sprague-Dawley rats and Beagle dogs
Arch Toxicol. 2024 Apr 12. doi: 10.1007/s00204-024-03746-x. Online ahead of print.ABSTRACTThe emergence of coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) led to a pandemic, prompting rapid vaccine development. Although vaccines are effective, the occurrence of rare adverse events following vaccination highlights the necessity of determining whether the benefits outweigh the risks posed by the infection itself. The recombinant Vesicular Stomatitis Virus (rVSV) platform is a promising vector for vaccines against emerging viruses. However, limited studies have evaluated t...
Source: Archives of Toxicology - April 12, 2024 Category: Toxicology Authors: Sang-Jin Park Heeseon Park Seng-Min Back Young Ju Lee Joung-Wook Seo Dabi Kim Jeong Hwa Lee Chaewon Kwak Kang-Hyun Han Hwa-Young Son Yong-Bum Kim Source Type: research
Neutralizing anti-diphtheria toxin scFv produced by phage display
CONCLUSION: This study describes the prosperous construction and isolation of scFv from the immune library, which specifically neutralizes diphtheria toxin. The HuscFv produced in this study can be a potential candidate to substitute the animal antibody for treating diphtheria and detecting toxins.PMID:38607601 | DOI:10.1007/s10529-024-03476-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - April 12, 2024 Category: Biotechnology Authors: Ehsan Khalili Mostafa Lakzaei Mahdi Aminian Source Type: research
Long-term performance of show-jumping horses and relationship with severity of ataxia and complications associated with myeloencephalopathy caused by equine herpes virus-1
CONCLUSIONS AND CLINICAL IMPORTANCE: Horses affected by EHM were able to return to their previous performance levels, but certain clinical variables were negatively associated with postoutbreak performance. Ataxia grade upon admission and the development of systemic signs of vasculitis and urinary complications were potential poor prognostic indicators in sport horses. Variables linked to fatality included prior vaccination against EHV-1, ataxia grade upon admission, and the development of urinary complications.PMID:38609161 | DOI:10.1111/jvim.17070 (Source: Herpes)
Source: Herpes - April 12, 2024 Category: Infectious Diseases Authors: Mar ía de la Cuesta-Torrado Ana Velloso Alvarez Patricia Neira-Egea Juan Cuervo-Arango Source Type: research
Genotoxicity and safety pharmacology of the rVSVInd(GML)-mspSGtc vaccine against SARS-CoV-2 in Sprague-Dawley rats and Beagle dogs
Arch Toxicol. 2024 Apr 12. doi: 10.1007/s00204-024-03746-x. Online ahead of print.ABSTRACTThe emergence of coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) led to a pandemic, prompting rapid vaccine development. Although vaccines are effective, the occurrence of rare adverse events following vaccination highlights the necessity of determining whether the benefits outweigh the risks posed by the infection itself. The recombinant Vesicular Stomatitis Virus (rVSV) platform is a promising vector for vaccines against emerging viruses. However, limited studies have evaluated t...
Source: Archives of Toxicology - April 12, 2024 Category: Toxicology Authors: Sang-Jin Park Heeseon Park Seng-Min Back Young Ju Lee Joung-Wook Seo Dabi Kim Jeong Hwa Lee Chaewon Kwak Kang-Hyun Han Hwa-Young Son Yong-Bum Kim Source Type: research
Neutralizing anti-diphtheria toxin scFv produced by phage display
CONCLUSION: This study describes the prosperous construction and isolation of scFv from the immune library, which specifically neutralizes diphtheria toxin. The HuscFv produced in this study can be a potential candidate to substitute the animal antibody for treating diphtheria and detecting toxins.PMID:38607601 | DOI:10.1007/s10529-024-03476-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - April 12, 2024 Category: Biotechnology Authors: Ehsan Khalili Mostafa Lakzaei Mahdi Aminian Source Type: research
Neutralizing anti-diphtheria toxin scFv produced by phage display
CONCLUSION: This study describes the prosperous construction and isolation of scFv from the immune library, which specifically neutralizes diphtheria toxin. The HuscFv produced in this study can be a potential candidate to substitute the animal antibody for treating diphtheria and detecting toxins.PMID:38607601 | DOI:10.1007/s10529-024-03476-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - April 12, 2024 Category: Biotechnology Authors: Ehsan Khalili Mostafa Lakzaei Mahdi Aminian Source Type: research
