Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic < em > Escherichia coli < /em >
The objective of this study was to prepare a novel tetravalent vaccine to effectively prevent piglet diarrhea caused by E. coli. In order to realize the production of K88ac-K99-ST1-LTB tetravalent inactivated vaccine, the biological characteristics, stability, preservation conditions, and safety of the recombinant strain BL21(DE3) (pXKKSL4) were studied, and the vaccine efficacy and minimum immune dose were measured. The results indicated that the biological characteristics, target protein expression, and immunogenicity of the 1st to 10th generations of the strain were stable. Therefore, the basic seed generation was preli...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: ChongLi Xu Yuhan She Fengyang Fu ChongBo Xu Source Type: research

Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic < em > Escherichia coli < /em >
The objective of this study was to prepare a novel tetravalent vaccine to effectively prevent piglet diarrhea caused by E. coli. In order to realize the production of K88ac-K99-ST1-LTB tetravalent inactivated vaccine, the biological characteristics, stability, preservation conditions, and safety of the recombinant strain BL21(DE3) (pXKKSL4) were studied, and the vaccine efficacy and minimum immune dose were measured. The results indicated that the biological characteristics, target protein expression, and immunogenicity of the 1st to 10th generations of the strain were stable. Therefore, the basic seed generation was preli...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: ChongLi Xu Yuhan She Fengyang Fu ChongBo Xu Source Type: research

Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic < em > Escherichia coli < /em >
The objective of this study was to prepare a novel tetravalent vaccine to effectively prevent piglet diarrhea caused by E. coli. In order to realize the production of K88ac-K99-ST1-LTB tetravalent inactivated vaccine, the biological characteristics, stability, preservation conditions, and safety of the recombinant strain BL21(DE3) (pXKKSL4) were studied, and the vaccine efficacy and minimum immune dose were measured. The results indicated that the biological characteristics, target protein expression, and immunogenicity of the 1st to 10th generations of the strain were stable. Therefore, the basic seed generation was preli...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: ChongLi Xu Yuhan She Fengyang Fu ChongBo Xu Source Type: research

Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic < em > Escherichia coli < /em >
The objective of this study was to prepare a novel tetravalent vaccine to effectively prevent piglet diarrhea caused by E. coli. In order to realize the production of K88ac-K99-ST1-LTB tetravalent inactivated vaccine, the biological characteristics, stability, preservation conditions, and safety of the recombinant strain BL21(DE3) (pXKKSL4) were studied, and the vaccine efficacy and minimum immune dose were measured. The results indicated that the biological characteristics, target protein expression, and immunogenicity of the 1st to 10th generations of the strain were stable. Therefore, the basic seed generation was preli...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: ChongLi Xu Yuhan She Fengyang Fu ChongBo Xu Source Type: research

Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic < em > Escherichia coli < /em >
The objective of this study was to prepare a novel tetravalent vaccine to effectively prevent piglet diarrhea caused by E. coli. In order to realize the production of K88ac-K99-ST1-LTB tetravalent inactivated vaccine, the biological characteristics, stability, preservation conditions, and safety of the recombinant strain BL21(DE3) (pXKKSL4) were studied, and the vaccine efficacy and minimum immune dose were measured. The results indicated that the biological characteristics, target protein expression, and immunogenicity of the 1st to 10th generations of the strain were stable. Therefore, the basic seed generation was preli...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: ChongLi Xu Yuhan She Fengyang Fu ChongBo Xu Source Type: research

Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic < em > Escherichia coli < /em >
The objective of this study was to prepare a novel tetravalent vaccine to effectively prevent piglet diarrhea caused by E. coli. In order to realize the production of K88ac-K99-ST1-LTB tetravalent inactivated vaccine, the biological characteristics, stability, preservation conditions, and safety of the recombinant strain BL21(DE3) (pXKKSL4) were studied, and the vaccine efficacy and minimum immune dose were measured. The results indicated that the biological characteristics, target protein expression, and immunogenicity of the 1st to 10th generations of the strain were stable. Therefore, the basic seed generation was preli...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: ChongLi Xu Yuhan She Fengyang Fu ChongBo Xu Source Type: research

Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic < em > Escherichia coli < /em >
The objective of this study was to prepare a novel tetravalent vaccine to effectively prevent piglet diarrhea caused by E. coli. In order to realize the production of K88ac-K99-ST1-LTB tetravalent inactivated vaccine, the biological characteristics, stability, preservation conditions, and safety of the recombinant strain BL21(DE3) (pXKKSL4) were studied, and the vaccine efficacy and minimum immune dose were measured. The results indicated that the biological characteristics, target protein expression, and immunogenicity of the 1st to 10th generations of the strain were stable. Therefore, the basic seed generation was preli...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: ChongLi Xu Yuhan She Fengyang Fu ChongBo Xu Source Type: research

Protective Efficacy of Lyophilized Vesicular Stomatitis Virus –Based Vaccines in Animal Model
A. Salawudeen et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - April 10, 2024 Category: Infectious Diseases Source Type: research

Study on chromatin regulation patterns of expression vectors in the PhiC31 integration site
Volume 19, Issue 1, December 2024 . (Source: Epigenetics)
Source: Epigenetics - April 10, 2024 Category: Genetics & Stem Cells Authors: Xueli LiuQina ChenXudong YinXiao WangJinshan RanWei YuBin Wanga Key Technology Engineering Center for New Veterinary Vaccine and Industry of Yunnan Provincial Education Department, Kunming University, Kunming, Yunnan, Chinab Pharmaceutical Department, Sec Source Type: research

Time, temperature and media: the three keys to improve the recovery of Campylobacter fetus subsp. venerealis from preputial bull samples
AbstractThe isolation ofCampylobacter fetus subsp.venerealis (Cfv) from clinical samples is the gold standard for confirming cases of bovine genital campylobacteriosis, an important cause of infertility in cattle and a potential public health concern. Furthermore, isolation is also necessary for the development of autologous vaccines, characterization of strains for antimicrobial susceptibility patterns, etc. Nevertheless, the sensitivity of culture methods is usually low, and there is no standardized protocol to maximize the recovery of Cfv from clinical samples. The aim of the current study is to design a protocol for th...
Source: Veterinary Research Communications - April 10, 2024 Category: Veterinary Research Source Type: research

Viruses, Vol. 16, Pages 571: Characterization of an African Swine Fever Virus Field Isolate from Vietnam with Deletions in the Left Variable Multigene Family Region
This study showed that ASFV-GUS-Vietnam field isolate is able to induce complete protection in the majority of the pigs against highly virulent homologous ASFV challenge, but has the potential for horizontal transmission, and can be fatal in some animals. This study highlights the need for proper monitoring and surveillance when ASFV live-attenuated virus-based vaccines are used in the field for ASF control in endemic countries. (Source: Viruses)
Source: Viruses - April 7, 2024 Category: Virology Authors: Aruna Ambagala Kalhari Goonewardene Ian El Kanoa Thi Tam Than Van Tam Nguyen Thi Ngoc Ha Lai Thi Lan Nguyen Cassidy N. G. Erdelyan Erin Robert Nikesh Tailor Chukwunonso Onyilagha Lindsey Lamboo Katherine Handel Michelle Nebroski Oksana Vernygora Oliver Lu Tags: Article Source Type: research

Pilot-scale production of a highly efficacious and stable monoglycosylated influenza split virus vaccine
In conclusion, this study provides efficacy and safety profiles of IVR-190mg for further clinical study and shows that this vaccine without a glycan shield has great potential to be safe and protective against H1N1 variants.PMID:38582606 | DOI:10.1016/j.vaccine.2023.11.038 (Source: Vaccine)
Source: Vaccine - April 6, 2024 Category: Allergy & Immunology Authors: Chia-Ying Wu Shao-En Kao Yung-Chieh Tseng Jen-Tzu Hou Li-Yang Wu Juine-Ruey Chen Source Type: research

In vitro assessment of the anthelmintic activity of copper oxide and zinc oxide nanoparticles on egg and adult stages of Fasciola hepatica: evidence on oxidative stress biomarkers, and DNA damage
Fasciolosis is of significant economic and public health importance worldwide. The lack of a successful vaccine and emerging resistance in flukes to the drug of choice, triclabendazole, has initiated the searc... (Source: BMC Veterinary Research)
Source: BMC Veterinary Research - April 4, 2024 Category: Veterinary Research Authors: Amirhassan Ravvaz, Farnaz Malekifard and Bijan Esmaeilnejad Tags: Research Source Type: research

Knowledge-attitude and practice of Anthrax and brucellosis: Implications for zoonotic disease surveillance and control in pastoral communities of Afar and Somali region, Ethiopia
ConclusionOur study revealed significant under-reporting of Anthrax and brucellosis, and weak prevention and response in humans, mostly associated with poor disease knowledge of healthcare providers. Ability to respond to animal outbreaks was limited by vaccine and drugs availability, timely vaccine administration and the mobility of pastoralists. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - April 4, 2024 Category: Tropical Medicine Authors: Rea Tschopp Source Type: research

A porcine kidney-derived clonal cell line with clear genetic annotation is highly susceptible to African swine fever virus
AbstractAfrican Swine Fever virus (ASFV), the causative agent of African swine fever, is a highly lethal hemorrhagic virus affecting domestic pigs and wild boars. The primary target cells for ASFV infection are porcine alveolar macrophages (PAMs), which are difficult to obtain and maintain in vitro, and less subjective to genetic editing. To overcome these issues and facilitate ASFV research, we obtained a subclonal cell line PK1-C5 by subcloning LLC-PK1 cells that support stable ASFV proliferation. This consequential cell line exhibited high ASFV infection levels and similar viral growth characteristics to PAMs, while als...
Source: Veterinary Research - April 4, 2024 Category: Veterinary Research Source Type: research