Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus in Xinjiang, China, from 2020 to 2022
In this study, we investigated the genetic characteristics of currently prevalent porcine epidemic diarrhea virus (PEDV) strains in the region. We collected 548 samples from animals with suspected PED on large-scale pig farms in Xinjiang. Of these, 258 tested positive for PEDV by RT-PCR, yielding an overall positivity rate of 47.08%.S1 gene sequencing and phylogenetic analysis were conducted on 23 randomly selected RT-PCR-positive samples. Three endemic strains of PEDV (PEDV/CH/XU/2020, PEDV/CH/XK/2020, and PEDV/CH/XA/2020) were isolated, and their complete genome sequences were analyzed for evidence of genetic recombinati...
Source: Archives of Virology - April 15, 2024 Category: Virology Source Type: research
Lactiplantibacillus plantarum surface-displayed VP6 (PoRV) protein can prevent PoRV infection in piglets
Int Immunopharmacol. 2024 Apr 13;133:112079. doi: 10.1016/j.intimp.2024.112079. Online ahead of print.ABSTRACTPorcine rotavirus (PoRV) poses a threat to the development of animal husbandry and human health, leading to substantial economic losses. VP6 protein is the most abundant component in virus particles and also the core structural protein of the virus. Firstly, this study developed an antibiotic-resistance-free, environmentally friendly expression vector, named asd-araC-PBAD-alr (AAPA). Then Recombinant Lactiplantibacillus plantarum (L. plantarum) strains induced by arabinose to express VP6 and VP6-pFc fusion proteins...
Source: International Immunopharmacology - April 14, 2024 Category: Allergy & Immunology Authors: Junhong Wang Haixu Wang Dongliang Zhang Fangyuan Liu Xiaoxu Li Ming Gao Mingyang Cheng Hongyu Bao Jiaxing Zhan Yan Zeng Chunfeng Wang Xin Cao Source Type: research
Lactiplantibacillus plantarum surface-displayed VP6 (PoRV) protein can prevent PoRV infection in piglets
Int Immunopharmacol. 2024 Apr 13;133:112079. doi: 10.1016/j.intimp.2024.112079. Online ahead of print.ABSTRACTPorcine rotavirus (PoRV) poses a threat to the development of animal husbandry and human health, leading to substantial economic losses. VP6 protein is the most abundant component in virus particles and also the core structural protein of the virus. Firstly, this study developed an antibiotic-resistance-free, environmentally friendly expression vector, named asd-araC-PBAD-alr (AAPA). Then Recombinant Lactiplantibacillus plantarum (L. plantarum) strains induced by arabinose to express VP6 and VP6-pFc fusion proteins...
Source: International Immunopharmacology - April 14, 2024 Category: Allergy & Immunology Authors: Junhong Wang Haixu Wang Dongliang Zhang Fangyuan Liu Xiaoxu Li Ming Gao Mingyang Cheng Hongyu Bao Jiaxing Zhan Yan Zeng Chunfeng Wang Xin Cao Source Type: research
Genotoxicity and safety pharmacology of the rVSVInd(GML)-mspSGtc vaccine against SARS-CoV-2 in Sprague-Dawley rats and Beagle dogs
Arch Toxicol. 2024 Apr 12. doi: 10.1007/s00204-024-03746-x. Online ahead of print.ABSTRACTThe emergence of coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) led to a pandemic, prompting rapid vaccine development. Although vaccines are effective, the occurrence of rare adverse events following vaccination highlights the necessity of determining whether the benefits outweigh the risks posed by the infection itself. The recombinant Vesicular Stomatitis Virus (rVSV) platform is a promising vector for vaccines against emerging viruses. However, limited studies have evaluated t...
Source: Archives of Toxicology - April 12, 2024 Category: Toxicology Authors: Sang-Jin Park Heeseon Park Seng-Min Back Young Ju Lee Joung-Wook Seo Dabi Kim Jeong Hwa Lee Chaewon Kwak Kang-Hyun Han Hwa-Young Son Yong-Bum Kim Source Type: research
Neutralizing anti-diphtheria toxin scFv produced by phage display
CONCLUSION: This study describes the prosperous construction and isolation of scFv from the immune library, which specifically neutralizes diphtheria toxin. The HuscFv produced in this study can be a potential candidate to substitute the animal antibody for treating diphtheria and detecting toxins.PMID:38607601 | DOI:10.1007/s10529-024-03476-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - April 12, 2024 Category: Biotechnology Authors: Ehsan Khalili Mostafa Lakzaei Mahdi Aminian Source Type: research
Long-term performance of show-jumping horses and relationship with severity of ataxia and complications associated with myeloencephalopathy caused by equine herpes virus-1
CONCLUSIONS AND CLINICAL IMPORTANCE: Horses affected by EHM were able to return to their previous performance levels, but certain clinical variables were negatively associated with postoutbreak performance. Ataxia grade upon admission and the development of systemic signs of vasculitis and urinary complications were potential poor prognostic indicators in sport horses. Variables linked to fatality included prior vaccination against EHV-1, ataxia grade upon admission, and the development of urinary complications.PMID:38609161 | DOI:10.1111/jvim.17070 (Source: Herpes)
Source: Herpes - April 12, 2024 Category: Infectious Diseases Authors: Mar ía de la Cuesta-Torrado Ana Velloso Alvarez Patricia Neira-Egea Juan Cuervo-Arango Source Type: research
Genotoxicity and safety pharmacology of the rVSVInd(GML)-mspSGtc vaccine against SARS-CoV-2 in Sprague-Dawley rats and Beagle dogs
Arch Toxicol. 2024 Apr 12. doi: 10.1007/s00204-024-03746-x. Online ahead of print.ABSTRACTThe emergence of coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) led to a pandemic, prompting rapid vaccine development. Although vaccines are effective, the occurrence of rare adverse events following vaccination highlights the necessity of determining whether the benefits outweigh the risks posed by the infection itself. The recombinant Vesicular Stomatitis Virus (rVSV) platform is a promising vector for vaccines against emerging viruses. However, limited studies have evaluated t...
Source: Archives of Toxicology - April 12, 2024 Category: Toxicology Authors: Sang-Jin Park Heeseon Park Seng-Min Back Young Ju Lee Joung-Wook Seo Dabi Kim Jeong Hwa Lee Chaewon Kwak Kang-Hyun Han Hwa-Young Son Yong-Bum Kim Source Type: research
Neutralizing anti-diphtheria toxin scFv produced by phage display
CONCLUSION: This study describes the prosperous construction and isolation of scFv from the immune library, which specifically neutralizes diphtheria toxin. The HuscFv produced in this study can be a potential candidate to substitute the animal antibody for treating diphtheria and detecting toxins.PMID:38607601 | DOI:10.1007/s10529-024-03476-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - April 12, 2024 Category: Biotechnology Authors: Ehsan Khalili Mostafa Lakzaei Mahdi Aminian Source Type: research
Neutralizing anti-diphtheria toxin scFv produced by phage display
CONCLUSION: This study describes the prosperous construction and isolation of scFv from the immune library, which specifically neutralizes diphtheria toxin. The HuscFv produced in this study can be a potential candidate to substitute the animal antibody for treating diphtheria and detecting toxins.PMID:38607601 | DOI:10.1007/s10529-024-03476-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - April 12, 2024 Category: Biotechnology Authors: Ehsan Khalili Mostafa Lakzaei Mahdi Aminian Source Type: research
Neutralizing anti-diphtheria toxin scFv produced by phage display
CONCLUSION: This study describes the prosperous construction and isolation of scFv from the immune library, which specifically neutralizes diphtheria toxin. The HuscFv produced in this study can be a potential candidate to substitute the animal antibody for treating diphtheria and detecting toxins.PMID:38607601 | DOI:10.1007/s10529-024-03476-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - April 12, 2024 Category: Biotechnology Authors: Ehsan Khalili Mostafa Lakzaei Mahdi Aminian Source Type: research
Neutralizing anti-diphtheria toxin scFv produced by phage display
CONCLUSION: This study describes the prosperous construction and isolation of scFv from the immune library, which specifically neutralizes diphtheria toxin. The HuscFv produced in this study can be a potential candidate to substitute the animal antibody for treating diphtheria and detecting toxins.PMID:38607601 | DOI:10.1007/s10529-024-03476-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - April 12, 2024 Category: Biotechnology Authors: Ehsan Khalili Mostafa Lakzaei Mahdi Aminian Source Type: research
Neutralizing anti-diphtheria toxin scFv produced by phage display
CONCLUSION: This study describes the prosperous construction and isolation of scFv from the immune library, which specifically neutralizes diphtheria toxin. The HuscFv produced in this study can be a potential candidate to substitute the animal antibody for treating diphtheria and detecting toxins.PMID:38607601 | DOI:10.1007/s10529-024-03476-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - April 12, 2024 Category: Biotechnology Authors: Ehsan Khalili Mostafa Lakzaei Mahdi Aminian Source Type: research
Redefining piscine lactococcosis
Appl Environ Microbiol. 2024 Apr 10:e0234923. doi: 10.1128/aem.02349-23. Online ahead of print.ABSTRACTPiscine lactococcosis is a significant threat to cultured and wild fish populations worldwide. The disease typically presents as a per-acute to acute hemorrhagic septicemia causing high morbidity and mortality, recalcitrant to antimicrobial treatment or management interventions. Historically, the disease was attributed to the gram-positive pathogen Lactococcus garvieae. However, recent work has revealed three distinct lactococcosis-causing bacteria (LCB)-L. garvieae, L. petauri, and L. formosensis-which are phenotypically...
Source: Applied and Environmental Microbiology - April 10, 2024 Category: Microbiology Authors: Taylor I Heckman Zeinab Yazdi Caitlin E Older Matt J Griffin Geoffrey C Waldbieser Alexander M Chow Isabella Medina Silva Kelsey M Anenson Julio C Garc ía Benjamin R LaFrentz Durda Slavic Kathy L Toohey-Kurth Paula Yant Heather M Fritz Eileen E Henderson Source Type: research
Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic < em > Escherichia coli < /em >
The objective of this study was to prepare a novel tetravalent vaccine to effectively prevent piglet diarrhea caused by E. coli. In order to realize the production of K88ac-K99-ST1-LTB tetravalent inactivated vaccine, the biological characteristics, stability, preservation conditions, and safety of the recombinant strain BL21(DE3) (pXKKSL4) were studied, and the vaccine efficacy and minimum immune dose were measured. The results indicated that the biological characteristics, target protein expression, and immunogenicity of the 1st to 10th generations of the strain were stable. Therefore, the basic seed generation was preli...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: ChongLi Xu Yuhan She Fengyang Fu ChongBo Xu Source Type: research
Redefining piscine lactococcosis
Appl Environ Microbiol. 2024 Apr 10:e0234923. doi: 10.1128/aem.02349-23. Online ahead of print.ABSTRACTPiscine lactococcosis is a significant threat to cultured and wild fish populations worldwide. The disease typically presents as a per-acute to acute hemorrhagic septicemia causing high morbidity and mortality, recalcitrant to antimicrobial treatment or management interventions. Historically, the disease was attributed to the gram-positive pathogen Lactococcus garvieae. However, recent work has revealed three distinct lactococcosis-causing bacteria (LCB)-L. garvieae, L. petauri, and L. formosensis-which are phenotypically...
Source: Applied and Environmental Microbiology - April 10, 2024 Category: Microbiology Authors: Taylor I Heckman Zeinab Yazdi Caitlin E Older Matt J Griffin Geoffrey C Waldbieser Alexander M Chow Isabella Medina Silva Kelsey M Anenson Julio C Garc ía Benjamin R LaFrentz Durda Slavic Kathy L Toohey-Kurth Paula Yant Heather M Fritz Eileen E Henderson Source Type: research
