Stability and integrity of self-assembled bovine parvovirus virus ‑like particles (BPV‑VLPs) of VP2 and combination of VP1VP2 assisted by baculovirus-insect cell expression: a potential logistical platform for vaccine deployment
Bovine parvovirus (BPV) is an autonomous DNA virus with a smaller molecular size and subtle differences in its structural proteins, unlike other animal parvoviruses. More importantly, this virus has the potent... (Source: Virology Journal)
Source: Virology Journal - April 19, 2024 Category: Virology Authors: Ashenafi Kiros Wubshet, Guo-xiu Li, Qian Li, Jun-Fei Dai, Yao-Zhong Ding, Luoyi Zhou, Min Qu, Yang Wang, Zhongyuan Ma, Gebremeskel Mamu Werid, Birhanu Hadush Abera, Asmelash Tassew Kebede, Yuefeng Sun, Xiangping Yin, Yongsheng Liu and Zhang Jie Tags: Methodology Source Type: research
Developing transmissible vaccines for animal infections | Science
Intrinsically safe designs and a staged transparent development process will be essential (Source: Science: Current Issue)
Source: Science: Current Issue - April 18, 2024 Category: Science Source Type: research
Cisd2 deficiency impairs neutrophil function by regulating calcium homeostasis via Calnexin and SERCA
BMB Rep. 2024 Apr 17:6179. Online ahead of print.ABSTRACTIn the context of aging, the susceptibility to infectious diseases increases, leading to heightened morbidity and mortality. This phenomenon, termed immunosenescence, is characterized by dysregulation in the aging immune system, including abnormal alterations in lymphocyte composition, elevated basal inflammation, and the accumulation of senescent T cells. Such changes contribute to increased autoimmune diseases, enhanced infection severity, and reduced responsiveness to vaccines. Utilizing aging animal models becomes imperative for a comprehensive understanding of i...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Un Yung Choi Youn Jung Choi Shin-Ae Lee Ji-Seung Yoo Source Type: research
Identifying major histocompatibility complex class II-DR molecules in bovine and swine peripheral blood monocyte-derived macrophages using mAb-L243
This study used mAb-L243 to identify Staphylococcus aureus and Salmonella enterica serovar Typhimurium peptides binding to cattle and swine macrophage MHC-II-DR molecules using flow cytometry, mass spectrometry and two immunopurification techniques. Antibody cross-reactivity led to identifying expressed MHC-II-DR molecules, together with 10 Staphylococcus aureus peptides in cattle and 13 S. enterica serovar Typhimurium peptides in swine. Such data demonstrates that MHC-II-DR expression and immunocapture approaches using L243 mAb represents a viable strategy for flow cytometry and immunopeptidomics analysis of bovine and sw...
Source: Vaccine - April 17, 2024 Category: Allergy & Immunology Authors: Carmen Celis-Giraldo Diego Ordo ñez Diana D íaz-Arévalo Michel D Boh órquez Nieves Ibarrola Carlos F Su árez Kewin Rodr íguez Yoelis Yepes Alexander Rodr íguez Catalina Avenda ño Julio L ópez-Abán Ra úl Manzano-Román Manuel Alfonso Patarroyo Source Type: research
Cisd2 deficiency impairs neutrophil function by regulating calcium homeostasis via Calnexin and SERCA
BMB Rep. 2024 Apr 17:6179. Online ahead of print.ABSTRACTIn the context of aging, the susceptibility to infectious diseases increases, leading to heightened morbidity and mortality. This phenomenon, termed immunosenescence, is characterized by dysregulation in the aging immune system, including abnormal alterations in lymphocyte composition, elevated basal inflammation, and the accumulation of senescent T cells. Such changes contribute to increased autoimmune diseases, enhanced infection severity, and reduced responsiveness to vaccines. Utilizing aging animal models becomes imperative for a comprehensive understanding of i...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Un Yung Choi Youn Jung Choi Shin-Ae Lee Ji-Seung Yoo Source Type: research
Identifying major histocompatibility complex class II-DR molecules in bovine and swine peripheral blood monocyte-derived macrophages using mAb-L243
This study used mAb-L243 to identify Staphylococcus aureus and Salmonella enterica serovar Typhimurium peptides binding to cattle and swine macrophage MHC-II-DR molecules using flow cytometry, mass spectrometry and two immunopurification techniques. Antibody cross-reactivity led to identifying expressed MHC-II-DR molecules, together with 10 Staphylococcus aureus peptides in cattle and 13 S. enterica serovar Typhimurium peptides in swine. Such data demonstrates that MHC-II-DR expression and immunocapture approaches using L243 mAb represents a viable strategy for flow cytometry and immunopeptidomics analysis of bovine and sw...
Source: Vaccine - April 17, 2024 Category: Allergy & Immunology Authors: Carmen Celis-Giraldo Diego Ordo ñez Diana D íaz-Arévalo Michel D Boh órquez Nieves Ibarrola Carlos F Su árez Kewin Rodr íguez Yoelis Yepes Alexander Rodr íguez Catalina Avenda ño Julio L ópez-Abán Ra úl Manzano-Román Manuel Alfonso Patarroyo Source Type: research
Cisd2 deficiency impairs neutrophil function by regulating calcium homeostasis via Calnexin and SERCA
BMB Rep. 2024 Apr 17:6179. Online ahead of print.ABSTRACTIn the context of aging, the susceptibility to infectious diseases increases, leading to heightened morbidity and mortality. This phenomenon, termed immunosenescence, is characterized by dysregulation in the aging immune system, including abnormal alterations in lymphocyte composition, elevated basal inflammation, and the accumulation of senescent T cells. Such changes contribute to increased autoimmune diseases, enhanced infection severity, and reduced responsiveness to vaccines. Utilizing aging animal models becomes imperative for a comprehensive understanding of i...
Source: BMB Reports - April 17, 2024 Category: Biochemistry Authors: Un Yung Choi Youn Jung Choi Shin-Ae Lee Ji-Seung Yoo Source Type: research
Effect of different levels of single cell protein and probiotic microorganisms on performance, immunological responses, and intestinal histology in laying hens
AbstractAn experiment was conducted to evaluate the effects of single-cell protein (SCP), as a valuable by-product and substitute for soybean meal, and multistrain probiotics on the performance, blood biochemistry, intestinal histomorphology, ileal microbiota, and immune responses in laying hens. Leghorn laying hens were randomly assigned to nine experimental treatments with eight replicates of five birds each. Dietary treatments consisted of three levels of replacement of soybean meal with SCP (0.0%, 30%, and 60%) and three levels of probiotics (0.0, 50, 100 mg/kg) offered through 70 days of main recording. The result ...
Source: Journal of Animal Physiology and Animal Nutrition - April 17, 2024 Category: Zoology Authors: Rasoul Rashedi Dehsahraee,
Amir Hossein Mahdavi,
Mohammad Sedghi,
Hassan Saleh Tags: ORIGINAL ARTICLE Source Type: research
Flock level socio-economic and other associated risk factors for Peste des petits ruminants (PPR) exposure in sheep and goats in Madhya Pradesh state, India
AbstractTo effectively control and eradicate PPR, the comprehensive understanding of risk factors associated with PPR exposure is vital. Hence, this study investigated socioeconomic and other associated risk determinants for PPR exposure at flock level in sheep and goats in a non-vaccination programme implemented Madhya Pradesh state India. A total of 410 sheep and goat flocks, comprised mostly of goats but also some mixed flocks, were surveyed during 2016 using a multistage random sampling procedure. Further, 230 blood samples were also collected from the farmers-reported PPR affected flocks and sera were tested using c-E...
Source: Tropical Animal Health and Production - April 16, 2024 Category: Veterinary Research Source Type: research
Development of a novel monoclonal antibody-based competitive ELISA for antibody detection against bovine leukemia virus
In conclusion, the novel p24 cELISA exhibited the potential to be a reliable and efficient diagnostic tool for BLV serological detection with a broad application prospect.PMID:38621561 | DOI:10.1016/j.ijbiomac.2024.131446 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - April 15, 2024 Category: Biochemistry Authors: Jing Wang Chao Sun Zhe Hu Fang Wang Jitao Chang Ming Gao Dandan Ye Qi Jia Hui Zou Luc Willems Zhigang Jiang Xin Yin Source Type: research
Cutting Edge: First Lung Infection Permanently Enlarges Lymph Nodes and Enhances New T Cell Responses
J Immunol. 2024 Apr 15:ji2400010. doi: 10.4049/jimmunol.2400010. Online ahead of print.ABSTRACTHumans experience frequent respiratory infections. Immunology and vaccinology studies in mice are typically performed in naive specific pathogen-free animals responding to their very first respiratory challenge. We found that the first respiratory infection induces lifelong enlargement of the lung-draining mediastinal lymph nodes (medLNs). Furthermore, infection-experienced medLNs supported better naive T cell surveillance and effector responses to new unrelated infections that exhibited more biased accumulation and memory establ...
Source: Journal of Immunology - April 15, 2024 Category: Allergy & Immunology Authors: J Michael Stolley Milcah C Scott Stephen D O'Flanagan Marco K ünzli Courtney A Matson Eyob Weyu Ryan A Langlois Vaiva Vezys David Masopust Source Type: research
Development of a novel monoclonal antibody-based competitive ELISA for antibody detection against bovine leukemia virus
In conclusion, the novel p24 cELISA exhibited the potential to be a reliable and efficient diagnostic tool for BLV serological detection with a broad application prospect.PMID:38621561 | DOI:10.1016/j.ijbiomac.2024.131446 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - April 15, 2024 Category: Biochemistry Authors: Jing Wang Chao Sun Zhe Hu Fang Wang Jitao Chang Ming Gao Dandan Ye Qi Jia Hui Zou Luc Willems Zhigang Jiang Xin Yin Source Type: research
Cutting Edge: First Lung Infection Permanently Enlarges Lymph Nodes and Enhances New T Cell Responses
J Immunol. 2024 Apr 15:ji2400010. doi: 10.4049/jimmunol.2400010. Online ahead of print.ABSTRACTHumans experience frequent respiratory infections. Immunology and vaccinology studies in mice are typically performed in naive specific pathogen-free animals responding to their very first respiratory challenge. We found that the first respiratory infection induces lifelong enlargement of the lung-draining mediastinal lymph nodes (medLNs). Furthermore, infection-experienced medLNs supported better naive T cell surveillance and effector responses to new unrelated infections that exhibited more biased accumulation and memory establ...
Source: Journal of Immunology - April 15, 2024 Category: Allergy & Immunology Authors: J Michael Stolley Milcah C Scott Stephen D O'Flanagan Marco K ünzli Courtney A Matson Eyob Weyu Ryan A Langlois Vaiva Vezys David Masopust Source Type: research
Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus in Xinjiang, China, from 2020 to 2022
In this study, we investigated the genetic characteristics of currently prevalent porcine epidemic diarrhea virus (PEDV) strains in the region. We collected 548 samples from animals with suspected PED on large-scale pig farms in Xinjiang. Of these, 258 tested positive for PEDV by RT-PCR, yielding an overall positivity rate of 47.08%.S1 gene sequencing and phylogenetic analysis were conducted on 23 randomly selected RT-PCR-positive samples. Three endemic strains of PEDV (PEDV/CH/XU/2020, PEDV/CH/XK/2020, and PEDV/CH/XA/2020) were isolated, and their complete genome sequences were analyzed for evidence of genetic recombinati...
Source: Archives of Virology - April 15, 2024 Category: Virology Source Type: research
Lactiplantibacillus plantarum surface-displayed VP6 (PoRV) protein can prevent PoRV infection in piglets
Int Immunopharmacol. 2024 Apr 13;133:112079. doi: 10.1016/j.intimp.2024.112079. Online ahead of print.ABSTRACTPorcine rotavirus (PoRV) poses a threat to the development of animal husbandry and human health, leading to substantial economic losses. VP6 protein is the most abundant component in virus particles and also the core structural protein of the virus. Firstly, this study developed an antibiotic-resistance-free, environmentally friendly expression vector, named asd-araC-PBAD-alr (AAPA). Then Recombinant Lactiplantibacillus plantarum (L. plantarum) strains induced by arabinose to express VP6 and VP6-pFc fusion proteins...
Source: International Immunopharmacology - April 14, 2024 Category: Allergy & Immunology Authors: Junhong Wang Haixu Wang Dongliang Zhang Fangyuan Liu Xiaoxu Li Ming Gao Mingyang Cheng Hongyu Bao Jiaxing Zhan Yan Zeng Chunfeng Wang Xin Cao Source Type: research
