Engineering a heterologous synthetic pathway in Escherichia coli for efficient production of biotin
CONCLUSION: Through introduction of heterologous biotin synthetic pathway, increasing the supply of precursor pimeloyl-CoA and cofactor SAM can significantly enhance biotin production in E. coli.PMID:33666816 | DOI:10.1007/s10529-021-03108-y (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 5, 2021 Category: Biotechnology Authors: Pei-Pei Wei Fu-Cheng Zhu Cun-Wu Chen Guo-Si Li Source Type: research

Optimization of activated charcoal detoxification and concentration of chestnut shell hydrolysate for xylitol production
CONCLUSIONS: Optimizing detoxification conditions with concentration level was found to be useful for enhancing xylitol production. In addition, fortification of the hydrolysate caused a three fold increase in maximum xylitol concentration.PMID:33651230 | DOI:10.1007/s10529-021-03087-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 2, 2021 Category: Biotechnology Authors: Kubra Eryasar-Orer Seda Karasu-Yalcin Source Type: research

Optimization of activated charcoal detoxification and concentration of chestnut shell hydrolysate for xylitol production
CONCLUSIONS: Optimizing detoxification conditions with concentration level was found to be useful for enhancing xylitol production. In addition, fortification of the hydrolysate caused a three fold increase in maximum xylitol concentration.PMID:33651230 | DOI:10.1007/s10529-021-03087-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 2, 2021 Category: Biotechnology Authors: Kubra Eryasar-Orer Seda Karasu-Yalcin Source Type: research

Optimization of activated charcoal detoxification and concentration of chestnut shell hydrolysate for xylitol production
CONCLUSIONS: Optimizing detoxification conditions with concentration level was found to be useful for enhancing xylitol production. In addition, fortification of the hydrolysate caused a three fold increase in maximum xylitol concentration.PMID:33651230 | DOI:10.1007/s10529-021-03087-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 2, 2021 Category: Biotechnology Authors: Kubra Eryasar-Orer Seda Karasu-Yalcin Source Type: research

Overexpression of uracil permease and nucleoside transporter from Bacillus amyloliquefaciens improves cytidine production in Escherichia coli
In this study, overexpression of uracil permease and a nucleoside transporter from Bacillus amyloliquefaciens related to cell membrane transport in Escherichia coli strain BG-08 was found to increase cytidine production in shake flask cultivation by 1.3-fold (0.91 ± 0.03 g/L) and 1.8-fold (1.26 ± 0.03 g/L) relative to that of the original strain (0.70 ± 0.03 g/L), respectively. Co-overexpression of uracil permease and a nucleoside transporter further increased cytidine yield by 2.7-fold (1.59 ± 0.05 g/L) compared with that of the original strain. These results indicate that the overexpressed ura...
Source: Biotechnology Letters - March 1, 2021 Category: Biotechnology Authors: Ruoshuang Ma Haitian Fang Huiyan Liu Lin Pan Hongyan Wang Heng Zhang Source Type: research

Overexpression of uracil permease and nucleoside transporter from Bacillus amyloliquefaciens improves cytidine production in Escherichia coli
In this study, overexpression of uracil permease and a nucleoside transporter from Bacillus amyloliquefaciens related to cell membrane transport in Escherichia coli strain BG-08 was found to increase cytidine production in shake flask cultivation by 1.3-fold (0.91 ± 0.03 g/L) and 1.8-fold (1.26 ± 0.03 g/L) relative to that of the original strain (0.70 ± 0.03 g/L), respectively. Co-overexpression of uracil permease and a nucleoside transporter further increased cytidine yield by 2.7-fold (1.59 ± 0.05 g/L) compared with that of the original strain. These results indicate that the overexpressed ura...
Source: Biotechnology Letters - March 1, 2021 Category: Biotechnology Authors: Ruoshuang Ma Haitian Fang Huiyan Liu Lin Pan Hongyan Wang Heng Zhang Source Type: research

A novel method for the in vitro assembly of virus-like particles and multimeric proteins
CONCLUSIONS: The novel method presented here is simpler and faster than other reported methods for the assembly and disassembly of viral proteins, a step needed for most applications.PMID:33638746 | DOI:10.1007/s10529-021-03093-2 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 27, 2021 Category: Biotechnology Authors: Mabel Rodr íguez Ricardo M Castro-Acosta Elias R Ruiz-Morales Francisca Villanueva-Flores Octavio T Ram írez Laura A Palomares Source Type: research

A novel method for the in vitro assembly of virus-like particles and multimeric proteins
CONCLUSIONS: The novel method presented here is simpler and faster than other reported methods for the assembly and disassembly of viral proteins, a step needed for most applications.PMID:33638746 | DOI:10.1007/s10529-021-03093-2 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 27, 2021 Category: Biotechnology Authors: Mabel Rodr íguez Ricardo M Castro-Acosta Elias R Ruiz-Morales Francisca Villanueva-Flores Octavio T Ram írez Laura A Palomares Source Type: research

Immunoproteomic analysis of Clostridium botulinum type B secretome for identification of immunogenic proteins against botulism
CONCLUSIONS: Identified immunogenic proteins can be used as vaccine candidates and diagnostic markers for the infant and wound botulism but common immunogenic proteins may be the best vaccine candidate molecule for development of vaccine as well as diagnostic system against the infant and wound botulism.PMID:33629143 | PMC:PMC7904509 | DOI:10.1007/s10529-021-03091-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 25, 2021 Category: Biotechnology Authors: Arti Sharma S Ponmariappan Sarita Rani S I Alam S Shukla Source Type: research

Immunoproteomic analysis of Clostridium botulinum type B secretome for identification of immunogenic proteins against botulism
CONCLUSIONS: Identified immunogenic proteins can be used as vaccine candidates and diagnostic markers for the infant and wound botulism but common immunogenic proteins may be the best vaccine candidate molecule for development of vaccine as well as diagnostic system against the infant and wound botulism.PMID:33629143 | DOI:10.1007/s10529-021-03091-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 25, 2021 Category: Biotechnology Authors: Arti Sharma S Ponmariappan Sarita Rani S I Alam S Shukla Source Type: research

Immunoproteomic analysis of Clostridium botulinum type B secretome for identification of immunogenic proteins against botulism
CONCLUSIONS: Identified immunogenic proteins can be used as vaccine candidates and diagnostic markers for the infant and wound botulism but common immunogenic proteins may be the best vaccine candidate molecule for development of vaccine as well as diagnostic system against the infant and wound botulism.PMID:33629143 | DOI:10.1007/s10529-021-03091-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 25, 2021 Category: Biotechnology Authors: Arti Sharma S Ponmariappan Sarita Rani S I Alam S Shukla Source Type: research

Mycelial culture extracts of selected wood-decay mushrooms as a source of skin-protecting factors
CONCLUSIONS: The results showed that the mycelial cultures of the studied species may be used as an alternative source of substances used in cosmetology.PMID:33624207 | DOI:10.1007/s10529-021-03095-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 24, 2021 Category: Biotechnology Authors: Katarzyna Su łkowska-Ziaja Karolina Grabowska Anna Apola Agata Kryczyk-Poprawa Bo żena Muszyńska Source Type: research

Mycelial culture extracts of selected wood-decay mushrooms as a source of skin-protecting factors
CONCLUSIONS: The results showed that the mycelial cultures of the studied species may be used as an alternative source of substances used in cosmetology.PMID:33624207 | DOI:10.1007/s10529-021-03095-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 24, 2021 Category: Biotechnology Authors: Katarzyna Su łkowska-Ziaja Karolina Grabowska Anna Apola Agata Kryczyk-Poprawa Bo żena Muszyńska Source Type: research

Correction to: Bio-electrospraying is a safe technology for delivering human adipose-derived stem cells
Biotechnol Lett. 2021 Feb 19. doi: 10.1007/s10529-021-03102-4. Online ahead of print.NO ABSTRACTPMID:33606122 | DOI:10.1007/s10529-021-03102-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 19, 2021 Category: Biotechnology Authors: Chuan Ye Zhixu He Yunfeng Lin Yi Zhang Jin Tang Bo Sun Minxian Ma Jielin Liu Long Yang Houxiang Ren Baoping Zhao Source Type: research

Correction to: Bio-electrospraying is a safe technology for delivering human adipose-derived stem cells.
PMID: 33606122 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 19, 2021 Category: Biotechnology Authors: Ye C, He Z, Lin Y, Zhang Y, Tang J, Sun B, Ma M, Liu J, Yang L, Ren H, Zhao B Tags: Biotechnol Lett Source Type: research

One-step purification and immobilization of recombinant proteins using SpyTag/SpyCatcher chemistry.
Abstract Based on the specific and spontaneous formation of isopeptide bonds by SpyCatcher/SpyTag, we have developed a one-step method for purification and immobilization of recombinant proteins. The procedure is to immobilize SpyCatcher on glyoxyl agarose gels, and then the SpyCatcher immobilisate can be used to immobilize the SpyTag-fused protein in the crude extract selectively. A mutant of SpyCatcher (mSC), in which a peptide (LysGlyLysGlyLysGly) was added to the C-terminus of SpyCatcher and three lysine residues around the SpyTag/SpyCatcher binding domain were replaced with arginine, was designed to improve t...
Source: Biotechnology Letters - February 16, 2021 Category: Biotechnology Authors: Tian J, Jia R, Wenge D, Sun H, Wang Y, Chang Y, Luo H Tags: Biotechnol Lett Source Type: research

Characterization of substrate specificity and inhibitory mechanism of bile salt hydrolase from Lactobacillus reuteri CRL 1098 using molecular docking analysis.
CONCLUSION: This study helps to delve into the molecular mechanisms involved in the recognition of substrates and potential inhibitors of LrBSH. PMID: 33591463 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 16, 2021 Category: Biotechnology Authors: Ledesma AE, Taranto MP, Bustos AY Tags: Biotechnol Lett Source Type: research

Furfural biotransformation in Acinetobacter baylyi ADP1 and Acinetobacter schindleri ACE.
CONCLUSIONS: Acinetobacter baylyi ADP1 is a candidate for the biological detoxification of furfural, a fermentation inhibitor present in lignocellulosic hydrolysates, with the possible direct involvement of the AreB and FrmA enzymes in the process. PMID: 33590377 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 15, 2021 Category: Biotechnology Authors: Arteaga JE, Cerros K, Rivera-Becerril E, Lara AR, Le Borgne S, Sigala JC Tags: Biotechnol Lett Source Type: research

Electrochemical characteristics of a gold nanoparticle-modified controlled enzyme-electrode contact junction electrode.
In this study, we postulate that the structural change of the enzyme would be reduced and the electrochemical performance improved by making the contact area between the enzyme and the electrode extremely small and adsorbing it as a point. Therefore, we aimed to develop a high-power biodevice that retains enzyme structure and activity by interposing gold nanoparticles (AuNPs) between the enzyme and the electrode. The enzymatic and electrochemical properties of pyrroloquinoline quinone-dependent glucose dehydrogenase adsorbed on AuNPs of 5-40 nm diameter were investigated. We found that the characteristics differed amo...
Source: Biotechnology Letters - February 12, 2021 Category: Biotechnology Authors: Mori S, Kitta Y, Sakamoto H, Takamura E, Suye SI Tags: Biotechnol Lett Source Type: research

Continuous production of fructooligosaccharides by recycling of the thermal-stable β-fructofuranosidase produced by Aspergillus niger.
CONCLUSIONS: The thermal-stable β-fructofuranosidase produced by A. niger can be recycled to achieve continuous synthesis of FOS with high efficiency, providing a powerful and economical strategy for the industrial production of FOS. PMID: 33575897 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 11, 2021 Category: Biotechnology Authors: Wang J, Zhang J, Wang L, Liu H, Li N, Zhou H, Ning Z, Zhang W, Wang L, Huang F, Zhong Y Tags: Biotechnol Lett Source Type: research

Performance evaluation of a novel conceptual bioprocess for clinically-required mass production of hematopoietic cells.
CONCLUSIONS: The results demonstrated that the conceptual bioprocess for suspended stem cell production has a great potential in practice although additional experiments are required to improve the system. PMID: 33554302 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 8, 2021 Category: Biotechnology Authors: Kaleybar LS, Khoshfetrat AB, Rahbarghazi R, Nozad Charoudeh H Tags: Biotechnol Lett Source Type: research

Surfactants: physicochemical interactions with biological macromolecules.
e;az De Rienzo MA Abstract Macromolecules are essential cellular components in biological systems responsible for performing a large number of functions that are necessary for growth and perseverance of living organisms. Proteins, lipids and carbohydrates are three major classes of biological macromolecules. To predict the structure, function, and behaviour of any cluster of macromolecules, it is necessary to understand the interaction between them and other components through basic principles of chemistry and physics. An important number of macromolecules are present in mixtures with surfactants, where a combinat...
Source: Biotechnology Letters - February 3, 2021 Category: Biotechnology Authors: Aguirre-Ramírez M, Silva-Jiménez H, Banat IM, Díaz De Rienzo MA Tags: Biotechnol Lett Source Type: research

Bufalin inhibits the malignant development of non-small cell lung cancer by mediating the circ_0046264/miR-522-3p axis.
CONCLUSION: Bufalin played an anti-tumor role in NSCLC by modulating the circ_0046264/miR-522-3p pathway, which might be a potential functional mechanism of bufalin in NSCLC. PMID: 33534015 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 3, 2021 Category: Biotechnology Authors: Jin J, Yao Z, Qin H, Wang K, Xin X Tags: Biotechnol Lett Source Type: research

Correction to: Overexpression of a Eutrema salsugineum phosphate transporter gene EsPHT1;4 enhances tolerance to low phosphorus stress in soybean.
PMID: 33534016 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 3, 2021 Category: Biotechnology Authors: Yang S, Feng Y, Zhao Y, Bai J, Wang J Tags: Biotechnol Lett Source Type: research

Design and development of a new ambr250 ® bioreactor vessel for improved cell and gene therapy applications.
Design and development of a new ambr250® bioreactor vessel for improved cell and gene therapy applications. Biotechnol Lett. 2021 Feb 02;: Authors: Rotondi M, Grace N, Betts J, Bargh N, Costariol E, Zoro B, Hewitt CJ, Nienow AW, Rafiq QA Abstract The emergence of cell and gene therapies has generated significant interest in their clinical and commercial potential. However, these therapies are prohibitively expensive to manufacture and can require extensive time for development due to our limited process knowledge and understanding. The automated ambr250® stirred-tank bioreactor platform provid...
Source: Biotechnology Letters - February 2, 2021 Category: Biotechnology Authors: Rotondi M, Grace N, Betts J, Bargh N, Costariol E, Zoro B, Hewitt CJ, Nienow AW, Rafiq QA Tags: Biotechnol Lett Source Type: research

Obtaining hemicellulosic hydrolysate from sugarcane bagasse for microbial oil production by Lipomyces starkeyi.
CONCLUSION: The hemicellulose hydrolysate from SCB is a potential substrate for L. starkeyi to produce lipids for biodiesel synthesis based on the biorefinery concept. PMID: 33517513 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 31, 2021 Category: Biotechnology Authors: da Cunha Abreu Xavier M, Teixeira Franco T Tags: Biotechnol Lett Source Type: research

Co-delivery of miRNA-15a and miRNA-16-1 using cationic PEGylated niosomes downregulates Bcl-2 and induces apoptosis in prostate cancer cells.
CONCLUSION: According to the results, it seems the designed niosomes containing miR-15a and miR-16-1 can target the Bcl-2 gene and provide a cheap, applicable, cost-effective, and safe drug delivery system against prostate cancer. PMID: 33515341 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 30, 2021 Category: Biotechnology Authors: Ghaffari M, Kalantar SM, Hemati M, Dehghani Firoozabadi A, Asri A, Shams A, Jafari Ghalekohneh S, Haghiralsadat F Tags: Biotechnol Lett Source Type: research

Expression and kinetic analysis of carboxylesterase LmCesA1 from Locusta migratoria.
CONCLUSIONS: These results suggest that overexpression of LmCesA1 enhances malathion sequestration to confer malathion tolerance in L. migratoria. PMID: 33511494 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 29, 2021 Category: Biotechnology Authors: Yin F, Ma W, Li D, Zhang X, Zhang J Tags: Biotechnol Lett Source Type: research

MPH-GST sensing microplate for easy detection of organophosphate insecticides.
CONCLUSIONS: All of the advantages of the MPH-GST sensing microplate developed have rendered it suitable for rapid and convenient OP screening, and for being a bio-element for fabricating a potential optical biosensor in the future. PMID: 33512614 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 29, 2021 Category: Biotechnology Authors: Rapichai W, Chaichalerm S, Mearnchu J, Wichitwechkarn J Tags: Biotechnol Lett Source Type: research

Bluemomycin, a new naphthoquinone derivative from Streptomyces sp. with antimicrobial and cytotoxic properties.
In this study, Streptomyces sp. (ERINLG-201) was isolated from the soil sample of Kodanad forest (Southern Western Ghats), The Nilgiris, Tamil Nadu, India. ERINLG-201 isolate showed promising antibacterial activity against tested Gram-positive and Gram-negative bacteria which was confirmed by perpendicular 'T' streak method. Secondary metabolites of ERINLG-201 isolate exhibited promising antibacterial activity against tested Gram-positive and Gram-negative bacteria which was confirmed by disc diffusion method using the ethylacetate extract. Further, the ethylacetate extract of ERINLG-201 (15 g) was packed in column ch...
Source: Biotechnology Letters - January 29, 2021 Category: Biotechnology Authors: Balachandran C, Al-Dhabi NA, Duraipandiyan V, Ignacimuthu S Tags: Biotechnol Lett Source Type: research

Quantitative proteomics implicates YggT in streptomycin resistance in Salmonella enterica serovar Enteritidis.
CONCLUSIONS: The above proteomic and mutagenic analyses revealed that yggT is involved in streptomycin resistance in S. enterica. PMID: 33502659 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 27, 2021 Category: Biotechnology Authors: Liang Y, Deng F, Mu P, Wen J, Deng Y Tags: Biotechnol Lett Source Type: research

Promoter engineering for high ectoine production in a lower saline medium by Halomonas hydrothermalis Y2.
CONCLUSIONS: As no impaired growth was observed in strain p/Y2/△ectD/△doeA while ectoine synthesis was increased, this promoter engineering strategy provides a practical protocol for lowering the salt-dependence of ectoine synthesis in this moderately halotolerant strain. PMID: 33502660 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 27, 2021 Category: Biotechnology Authors: Li S, Shang Y, Zhao Q, Liu Y, Dong X, Wang W, Yang C Tags: Biotechnol Lett Source Type: research

Enrichment of marine manganese-oxidizing microorganisms using polycaprolactone as a solid organic substrate.
CONCLUSIONS: Our data demonstrate that PCL can serve as a potential substrate to enrich Mn-oxidizing microorganisms with the ability to produce MnOx under marine conditions. PMID: 33496920 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 26, 2021 Category: Biotechnology Authors: Aoki M, Miyashita Y, Tran PT, Okuno Y, Watari T, Yamaguchi T Tags: Biotechnol Lett Source Type: research

Circular RNA 0081146 facilitates the progression of gastric cancer by sponging miR-144 and up-regulating HMGB1.
CONCLUSION: Circ_0081146 functions as an oncogene in GC to promote cell growth, migration and invasion via modulating the miR-144/HMGB1 axis. PMID: 33496921 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 26, 2021 Category: Biotechnology Authors: Xu Q, Liao B, Hu S, Zhou Y, Xia W Tags: Biotechnol Lett Source Type: research

The carbon partitioning of glucose and DIC in mixotrophic, heterotrophic and photoautotrophic cultures of Tetraselmis suecica.
CONCLUSIONS: There is a condition specific shift in carbon partitioning into different fatty acid and biochemical fractions in T. suecica, with more inorganic carbon partitioned into 18:2 n-(9,12) in photoautotrophic rather than mixotrophic cultures. PMID: 33459952 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 18, 2021 Category: Biotechnology Authors: Penhaul Smith JK, Hughes AD, McEvoy L, Thornton B, Day JG Tags: Biotechnol Lett Source Type: research

Differential proteomics reveals main determinants for the improved pectinase activity in UV-mutagenized Aspergillus niger strain.
CONCLUSIONS: The result demonstrated that the improved pectinase activity by UV-mutagenesis in A. niger EIMU2 was probably contributed to the up-regulated expression of rhamnogalacturonan lyase, or pectinesterase, which resulted in the optimization of synergy amongst different components of pectin-degrading enzymes. PMID: 33449253 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 15, 2021 Category: Biotechnology Authors: Lin W, Xu X, Lv R, Huang W, Ul Haq H, Gao Y, Ren H, Lan C, Tian B Tags: Biotechnol Lett Source Type: research

A brief period of darkness induces changes in fatty acid biosynthesis towards accumulation of saturated fatty acids in Chlorella vulgaris UMT-M1 at stationary growth phase.
CONCLUSIONS: Findings from this study suggests that fatty acid composition in C. vulgaris could be modulated to favor either C16:0 or C18:1 by a brief period of either darkness or light incubation, prior to harvesting. PMID: 33438120 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 12, 2021 Category: Biotechnology Authors: Cha TS, Yee W, Phua PSP, Loh SH, Aziz A Tags: Biotechnol Lett Source Type: research

Improved secretion of recombinant human IL-25 in HEK293 cells using a signal peptide-pro-peptide domain derived from Trypsin-1.
CONCLUSION: The Trypsin-1 signal peptide-pro-peptide sequence increased human IL-25 expression and secretion in mammalian cells by fivefold. PMID: 33415569 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 7, 2021 Category: Biotechnology Authors: Mullin MJ, Wilkinson C, Hiles I, Smith KJ Tags: Biotechnol Lett Source Type: research

Circ_0114876 promoted IL-1 β-induced chondrocyte injury by targeting miR-671/TRAF2 axis.
Circ_0114876 promoted IL-1β-induced chondrocyte injury by targeting miR-671/TRAF2 axis. Biotechnol Lett. 2021 Jan 06;: Authors: Wang Q, Luo S, Yang J, Li J, Huan S, She G, Zha Z Abstract Osteoarthritis (OA) is a chronic joint disease, which occurs in the elderly. The regulatory mechanisms of circRNAs were involved in the occurrence and development of various diseases. However, the potential regulatory network of circRNA in OA remains further research and clarification. The expression of circ_0114876 was increased in OA tissues and inhibition of circ_0114876 could induce cell viability and suppres...
Source: Biotechnology Letters - January 6, 2021 Category: Biotechnology Authors: Wang Q, Luo S, Yang J, Li J, Huan S, She G, Zha Z Tags: Biotechnol Lett Source Type: research

Bacillus subtilis CtpxS2-1 induces systemic resistance against anthracnose in Andean lupin by lipopeptide production.
CONCLUSION: B. subtilis CtpxS2-1 is a key factor enhancing Andean lupin health by producing lipopeptides that damage C. acutatum cellular structures and inhibit their growth, as well as by inducing the expression of defence-related genes of lupin plants involved in systemic acquired resistance (SAR) against anthracnose. PMID: 33389271 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 3, 2021 Category: Biotechnology Authors: Yánez-Mendizábal V, Falconí CE Tags: Biotechnol Lett Source Type: research

Box-Behnken design optimisation of a green novel nanobio-based reagent for rapid visualisation of latent fingerprints on wet, non-porous substrates.
CONCLUSION: The optimised novel NBR could be the relatively greener option for visualising latent fingerprints on wet, non-porous substrates for forensic applications. PMID: 33389272 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 3, 2021 Category: Biotechnology Authors: Azman AR, Mahat NA, Wahab RA, Ahmad WA, Puspanadan JK, Huri MAM, Kamaluddin MR, Ismail D Tags: Biotechnol Lett Source Type: research

CT5, a subtle in vitro DNA assembling method based on the combination of FnCas12a and T5 exonuclease.
CONCLUSIONS: The CT5 method was a precise and exquisite DNA operating system and provided an ideal platform for the study of gene functions, genetic engineering and synthetic biology. PMID: 33389273 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 3, 2021 Category: Biotechnology Authors: Dong M, Wang F, Lv B, Mei M, Ma L, Hu Y, Zhai C Tags: Biotechnol Lett Source Type: research

Growth of Leucoagaricus gongylophorus M öller (Singer) and production of key enzymes in submerged and solid-state cultures with lignocellulosic substrates.
Growth of Leucoagaricus gongylophorus Möller (Singer) and production of key enzymes in submerged and solid-state cultures with lignocellulosic substrates. Biotechnol Lett. 2021 Jan 02;: Authors: Maya-Yescas ME, Revah S, Le Borgne S, Valenzuela J, Palacios-González E, Terrés-Rojas E, Vigueras-Ramírez G Abstract The aim of this study was to characterize the growth of the fungus Leucoagaricus gongylophorus LEU18496, isolated from the fungus garden of the nest of leaf cutter ants Atta mexicana. The fungus garden was cultivated in an artificial laboratory nest and the fungus furth...
Source: Biotechnology Letters - January 2, 2021 Category: Biotechnology Authors: Maya-Yescas ME, Revah S, Le Borgne S, Valenzuela J, Palacios-González E, Terrés-Rojas E, Vigueras-Ramírez G Tags: Biotechnol Lett Source Type: research

Suspension state regulates epithelial-to-mesenchymal transition and stemness of breast tumor cells.
CONCLUSION: The study highlighted the emergence of hybrid epithelial/mesenchymal (E/M) state during hematogenous metastasis and the plasticity of CTCs caused by cancer stem cells, further providing novel insights into clinical monitoring of CTCs and therapeutic strategies. PMID: 33386502 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 2, 2021 Category: Biotechnology Authors: Lv Y, Zhang X, Chen L Tags: Biotechnol Lett Source Type: research

Production and characterization of a novel, thermotolerant fungal phytase from agro-industrial byproducts for cattle feed.
CONCLUSION: Overall, our report highlights the production of an efficient and thermotolerant phytase with potential as a low-cost animal feed supplement. PMID: 33387113 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 2, 2021 Category: Biotechnology Authors: Kumari N, Bansal S Tags: Biotechnol Lett Source Type: research

Characterization of Lactobacillus reuteri WQ-Y1 with the ciprofloxacin degradation ability.
CONCLUSIONS: L. reuteri WQ-Y1 could degrade fluoroquinolone antibiotics with the similar piperazine ring structure. However, future work still needs to be done on the confirmation of the key enzymes in the cytochrome P450 enzymes family in the biodegradation. The isolated ciprofloxacin-degrading strain L. reuteri WQ-Y1 had a CIP degradation rate of 65.1% at 24 hours, and one biodegradation metabolite was identified and proved to be an important metabolite of CIP from cytochrome P450 enzymes family hydrolysis with UPLC-MS/MS spectrograms approach. PMID: 33387114 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 2, 2021 Category: Biotechnology Authors: Qu C, Wu Z, Pan D, Cai Z, Liu X Tags: Biotechnol Lett Source Type: research

Synthesis of novel nicotinamide susbstituted phthalocyanine and photodynamic antomicrobial chemotherapy evaluation potentiated by potassium iodide against the gram positive S. aureus and gram negative E. coli.
Abstract In the present work, we propose the synthesis of novel nicotinamide subsituted phthlocyanine photosensitizer (PS) and characterized by FTIR, UV-visible, H-NMR and MALDI Toff spectroscopy. Nicotinamide plays a vital rule in the central nervous system and its potential as a therapeutic for neurodegenerative disease. Nicotinamide substituted PS (3) efficiently produced ROS via type-1 process as measured by DCF assay. We observed that our PS after red light illumination (22 J/cm2) killed gram positive S. aureus upto 3 log reduction. Furher the addition of Potassium Iodide (100 mM) significantly pote...
Source: Biotechnology Letters - January 2, 2021 Category: Biotechnology Authors: Ullah A, Zhao T, Muhammad MT, Khan M, Qurban S, Rahman A, Ahmad I Tags: Biotechnol Lett Source Type: research

Circ_0001247 functions as a miR-1270 sponge to accelerate cervical cancer progression by up-regulating ZEB2 expression level.
CONCLUSION: Circ_0001247 promotes progression of CC by sponging miR-1270 to upregulate ZEB2 expression level. PMID: 33386495 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 1, 2021 Category: Biotechnology Authors: Wang W, Xu A, Zhao M, Sun J, Gao L Tags: Biotechnol Lett Source Type: research

Microbial bioconversion of feathers into antioxidant peptides and pigments and their liposome encapsulation.
CONCLUSIONS: Feather waste can be a valuable substrate for simultaneous production of antioxidant peptides and pigment by Chryseobacterium sp. kr6, and their encapsulation into liposomes may be a suitable alternative for delivery of these natural antioxidants. PMID: 33386496 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 1, 2021 Category: Biotechnology Authors: Bertolini D, Jiménez MEP, Dos Santos C, Corrêa APF, Brandelli A Tags: Biotechnol Lett Source Type: research

Characterization and inhibition of four fungi producing citrinin in various culture media.
CONCLUSIONS: These results indicated that the suitable fermentation conditions could make considerable contributions to the reduction of citrinin production. This study provided an effective way for decreasing the citrinin production. PMID: 33386497 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 1, 2021 Category: Biotechnology Authors: Gu S, Chen Z, Wang F, Wang X Tags: Biotechnol Lett Source Type: research