Co-evolution of β-glucosidase activity and product tolerance for increasing cellulosic ethanol yield.
Co-evolution of β-glucosidase activity and product tolerance for increasing cellulosic ethanol yield. Biotechnol Lett. 2020 Jun 24;: Authors: Wang K, Huang Q, Li H, Zhao X Abstract β-Glucosidase (BGL) plays a key role in cellulose hydrolysis. However, it is still a great challenge to enhance product tolerance and enzyme activity of BGL simultaneously. Here, we utilized one round error-prone PCR to engineer the Penicillium oxalicum 16 BGL (16BGL) for improving the cellulosic ethanol yield. We identified a new variant (L-6C), a triple mutant (M280T/V484L/D589E), with enhanced catalytic efficie...
Source: Biotechnology Letters - June 24, 2020 Category: Biotechnology Authors: Wang K, Huang Q, Li H, Zhao X Tags: Biotechnol Lett Source Type: research

Enhancement of S-adenosylmethionine production by deleting thrB gene and overexpressing SAM2 gene in Bacillus amyloliquefaciens.
CONCLUSIONS: Deleting thrB and overexpressing SAM2 gene were efficient for enhanced SAM production in B. amyloliquefaciens. The maximum SAM titer in flasks batch fermentation was much higher than that of previous reports. PMID: 32577851 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 23, 2020 Category: Biotechnology Authors: Jiang C, Ruan L, Wei X, Guo A Tags: Biotechnol Lett Source Type: research

Investigation of the gut microbiome of Apis cerana honeybees from Vietnam.
CONCLUSIONS: The presence of beneficial bacteria in the gut highlighted their role in the honeybee and suggested that they can be promising candidates for the development of probiotics for health improvement, infection control and disease management of honeybees. PMID: 32577852 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 23, 2020 Category: Biotechnology Authors: Duong BTT, Lien NTK, Thu HT, Hoa NT, Lanh PT, Yun BR, Yoo MS, Cho YS, Van Quyen D Tags: Biotechnol Lett Source Type: research

Aerobic biodegradation of high explosive hexahydro-1,3,5- trinitro-1,3,5-triazine by Janibacter cremeus isolated from contaminated soil.
CONCLUSION: J. cremeus could degrade RDX as sole source of nitrogen, via three different pathways wherein, Nitroreductase enzyme was found to play a major role. The efficient degradation of RDX makes J. cremeus suitable in treatment of contaminated water and soil at field scale levels. PMID: 32572651 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 22, 2020 Category: Biotechnology Authors: Kalsi A, Celin SM, Sharma JG Tags: Biotechnol Lett Source Type: research

Recombinant ferritin nanoparticles can induce dendritic cell maturation through TLR4/NF- κB pathway.
CONCLUSION: RFNps induced DCs maturation lends the potential application of RFNps as carrier platforms in DC-based vaccine. PMID: 32567013 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 22, 2020 Category: Biotechnology Authors: Qu Z, Guo Y, Li M, Cao C, Wang J, Gao M Tags: Biotechnol Lett Source Type: research

Correction to: Bioconversion of arachidonic acid into human 14,15-hepoxilin B3 and 13,14,15-trioxilin B3 by recombinant cells expressing microbial 15-lipoxygenase without and with epoxide hydrolase.
Abstract In the original publication of the article, some chirality of Fig. 1 was published incorrectly. The corrected figure is provided below. PMID: 32564168 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 20, 2020 Category: Biotechnology Authors: Lee J, Kim TH, Oh DK Tags: Biotechnol Lett Source Type: research

By targeting TRAF6, miR-140-3p inhibits TGF- β1-induced human osteosarcoma epithelial-to-mesenchymal transition, migration, and invasion.
CONCLUSION: These results demonstrate miR-140-3p to function as a tumor inhibitor of human OS cells by decreasing TRAF6 expression. miR-140-3p and TRAF6 may be valuable and novel biomarkers for diagnosis and treatment of OS. PMID: 32562135 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 19, 2020 Category: Biotechnology Authors: Guo Q, Zhang N, Liu S, Pang Z, Chen Z Tags: Biotechnol Lett Source Type: research

Engineered formate dehydrogenase from Chaetomium thermophilum, a promising enzymatic solution for biotechnical CO2 fixation.
CONCLUSIONS: Two variants presenting up to five-fold increase in catalytic efficiency and kcat were obtained and characterised. They constitute a promising enzymatic alternative for CO2 utilization and will serve as scaffolds to be further developed in order to meet industrial requirements. PMID: 32557118 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 16, 2020 Category: Biotechnology Authors: Çakar MM, Ruupunen J, Mangas-Sanchez J, Birmingham WR, Yildirim D, Turunen O, Turner NJ, Valjakka J, Binay B Tags: Biotechnol Lett Source Type: research

Purification and characterization of a native lytic polysaccharide monooxygenase from Thermoascus aurantiacus.
Abstract Lytic polysaccharide monooxygenases (LPMOs) have emerged as key proteins for depolymerization of cellulose. These copper-containing enzymes oxidize C-1 and/or C-4 bonds in cellulose, promoting increased hydrolysis of the oxidized cellulose chains. The LPMO from Thermoascus aurantiacus, a thermophilic ascomycete fungus, has been extensively studied and has served as a model LPMO. A method was developed to purify the LPMO from culture filtrates of T. aurantiacus along with its native cellobiohydrolase and endoglucanase. The activity of the purified LPMO was measured with a colorimetric assay that establishe...
Source: Biotechnology Letters - June 15, 2020 Category: Biotechnology Authors: Fritsche S, Hopson C, Gorman J, Gabriel R, Singer SW Tags: Biotechnol Lett Source Type: research

Inhibition of berberine hydrochloride on Candida albicans biofilm formation.
This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation. RESULTS: This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we found that 128 and 32 μg/mL BH significantly inhibited biofilm formation (P 
Source: Biotechnology Letters - June 15, 2020 Category: Biotechnology Authors: Huang X, Zheng M, Yi Y, Patel A, Song Z, Li Y Tags: Biotechnol Lett Source Type: research

A novel system to rapidly detect protein-protein interactions (PPIs) based on fluorescence co-localization.
CONCLUSIONS: There FTCL system provided a novel detection method for PPIs, regardless of protein localization in the nucleus or cytoplasm. More importantly, this study provides a new strategy for future protein interaction studies through organelle localization (such as mitochondria, Golgi and cytomembrane, etc.). PMID: 32533375 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 13, 2020 Category: Biotechnology Authors: Hu N, Dong ZQ, Chen TT, Zheng N, Wu Q, Chen P, Lu C, Pan MH Tags: Biotechnol Lett Source Type: research

Production and characterization of an enzyme extract with cellulase activity produced by an indigenous strain of Fusarium verticillioides ITV03 using sweet sorghum bagasse.
CONCLUSION: The present work shows that a native strain like F. verticillioides ITV03 using DSSB supplemented with nitrogen has a great potential as a producer of cellulase for lignocellulosic residue hydrolysis. PMID: 32533374 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 12, 2020 Category: Biotechnology Authors: Infanzón-Rodríguez MI, Ragazzo-Sánchez JA, Del Moral S, Calderón-Santoyo M, Aguilar-Uscanga MG Tags: Biotechnol Lett Source Type: research

Overexpression and characterization of a novel GH16 β-agarase (Aga1) from Cellulophaga omnivescoria W5C.
CONCLUSIONS: Aga1 displays unique traits such as moderate psychrophilicity, stability, and synergy with other agarases, which makes it an excellent candidate for biosynthetic production of neoagarooligosaccharides from agar. PMID: 32519168 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 9, 2020 Category: Biotechnology Authors: Ramos KRM, Valdehuesa KNG, Bañares AB, Nisola GM, Lee WK, Chung WJ Tags: Biotechnol Lett Source Type: research

Neuroprotection of melatonin on spinal cord injury by activating autophagy and inhibiting apoptosis via SIRT1/AMPK signaling pathway.
Abstract The effect of melatonin (MT) on spinal cord injury (SCI) has attracted increasing research attention. However, the specific role and molecular mechanism of MT on SCI have not been elucidated. An experiment was performed to investigate the effect and molecular mechanism of MT on the neuronal autophagy after SCI and its effect on the recovery of nerve function. The rats were randomly divided into four treatment groups: the SCI+MT+EX527 (SIRT1 inhibitor), SCI+MT, SCI, and sham operation groups. On the 14th day after SCI, MT significantly promoted the recovery of motor function in the hind limbs according to ...
Source: Biotechnology Letters - June 8, 2020 Category: Biotechnology Authors: Gao K, Niu J, Dang X Tags: Biotechnol Lett Source Type: research

Improvement of the recombinant human coagulation factor IX expression by co-expression of a novel transcript of Drosophila γ carboxylase in a human cell line.
Improvement of the recombinant human coagulation factor IX expression by co-expression of a novel transcript of Drosophila γ carboxylase in a human cell line. Biotechnol Lett. 2020 Jun 08;: Authors: Moniri Javadhesari S, Zomorodipour A Abstract OBJECTIVE: Mammalian cells as the main host for production of human proteins are incapable of complete γ-carboxylation of over-expressed Vitamin K Dependent (VKD) proteins. The Drosophila γ-glutamyl carboxylase (DγC) has been shown to be more efficient than its human counterpart in γ-carboxylation of human substrates, in vitro. Con...
Source: Biotechnology Letters - June 8, 2020 Category: Biotechnology Authors: Moniri Javadhesari S, Zomorodipour A Tags: Biotechnol Lett Source Type: research

Formaldehyde effects on kanamycin resistance gene of inactivated recombinant Escherichia coli vaccines.
CONCLUSIONS: We found that this compound is effective as an inactivation agent. Here we demonstrate the biosafety involving antibiotic resistance gene of recombinant E. coli vaccines allowing to industrial production and animal application. PMID: 32500473 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 4, 2020 Category: Biotechnology Authors: Donassolo RA, Ferreira MRA, Moreira C, Dos Santos LM, Griep E, Moreira GMSG, Rodrigues RR, Moreira ÂN, Conceição FR Tags: Biotechnol Lett Source Type: research

Piperine inhibits colorectal cancer migration and invasion by regulating STAT3/Snail-mediated epithelial-mesenchymal transition.
Abstract Cancer metastasis is the primary cause of death in patients diagnosed with colorectal cancer. Piperine, an active nontoxic ingredient in pepper, has potent anti-inflammatory and anti-cancer properties. However, little is known about the anti-migratory and anti-invasive effects of piperine on colorectal cancer. We demonstrated piperine inhibited the migration and invasion of colorectal cancer cells. Then, we found piperine reversed the biomarker expression of epithelial-to-mesenchymal transition (EMT), and suppressed the EMT regulator Snail. Furthermore, signal transducers and activators of transcription 3...
Source: Biotechnology Letters - June 4, 2020 Category: Biotechnology Authors: Song L, Wang Y, Zhen Y, Li D, He X, Yang H, Zhang H, Liu Q Tags: Biotechnol Lett Source Type: research

Nanoparticle technology improves in-vitro attachment of cattle (Bos taurus) trophectoderm cells.
Abstract The bovine cell line, cow trophectoderm-1 (CT-1), provides an excellent in-vitro cell culture model to study early embryonic development. Obtaining consistent attachment and outgrowth, however, is difficult because enzymatic disassociation into single cells is detrimental; therefore, CT-1 cells must be passaged in clumps, which do not attach readily to the surface of the dish. We tested whether magnetic nanoparticles, NanoShuttle™-PL, could be used to improve cell attachment and subsequent proliferation of the cattle trophectoderm cell line without altering cellular metabolism or immunofluorescent d...
Source: Biotechnology Letters - June 3, 2020 Category: Biotechnology Authors: Chung J, Sriram G, Keefer CL Tags: Biotechnol Lett Source Type: research

Efficient CRISPR/Cas9-mediated gene editing in Guangdong small-ear spotted pig cells using an optimized electrotransfection method.
CONCLUSIONS: This study establishes the foundation for genetic improvement and resource development of GDSS pigs using CRISPR/Cas9-mediated gene editing combined with SCNT. PMID: 32494996 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 3, 2020 Category: Biotechnology Authors: Wei YY, Zhan QM, Zhu XX, Yan AF, Feng J, Liu L, Li JH, Tang DS Tags: Biotechnol Lett Source Type: research

Improving the thermostability of a GH97 dextran glucosidase by rational design.
This study was aimed at improving the thermostability of dextran glucosidase PspAG97A, a member of the glycoside hydrolase family 97, from Pseudoalteromonas sp. K8. A total of 9 lysine residues were chosen using the TKSA-MC program based on the optimization of surface charge-charge interactions and were mutated to glutamate for shifting the enzyme's isoelectric point off its optimum pH value. Three mutants K75E, K363E and K420E showed enhanced thermostability. The triple mutant, K75E/K363E/K420E, was found to be the best with a 7.3-fold increase in half-life (t1/2) at 33 °C compared to that of the wild-type (WT). ...
Source: Biotechnology Letters - June 1, 2020 Category: Biotechnology Authors: Zhang X, Chen F, He C, Fang W, Fang Z, Zhang X, Wang X, Xiao Y Tags: Biotechnol Lett Source Type: research

Bioconversion of arachidonic acid into human 14,15-hepoxilin B3 and 13,14,15-trioxilin B3 by recombinant cells expressing microbial 15-lipoxygenase without and with epoxide hydrolase.
CONCLUSION: These are the highest concentrations, productivities, and yields of hepoxilin and trioxilin from ARA reported thus far. PMID: 32488442 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 1, 2020 Category: Biotechnology Authors: Lee J, Kim TH, Oh DK Tags: Biotechnol Lett Source Type: research

Continuous biomass and lipid production from local chlorella-bacteria consortium in raw wastewater using volatile fatty acids.
CONCLUSIONS: Although bacterial quantities increased with algae growth concurrently, the objective of culturing microalgae-bacteria consortium in raw wastewater without sterilization to produce biomass and lipid yield still can be realized. PMID: 32488443 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 1, 2020 Category: Biotechnology Authors: Zuo Y, Wongsnansilp T, Zhang X, Chen G, Wu Z Tags: Biotechnol Lett Source Type: research

Codon usage trend in genes associated with obesity.
Abstract Obesity is not only a social menace but also an economic burden as it reduces productivity and increases health care cost. We used bioinformatic tools to analyze the CUB of obesity associated genes and compared with housekeeping genes (control) to explore the similarities and differences between two data sets as no work was reported yet. The mean effective number of codons (ENC) in genes associated with obesity and housekeeping gene was 50.45 and 52.03 respectively, indicating low CUB. The relative synonymous codon usage (RSCU) suggested that codons namely CTG and GTG were over-represented in both obesity...
Source: Biotechnology Letters - June 1, 2020 Category: Biotechnology Authors: Chakraborty S, Barbhuiya PA, Paul S, Uddin A, Choudhury Y, Ahn Y, Cho YS Tags: Biotechnol Lett Source Type: research

MicroRNA miR-212 regulates PDCD4 to attenuate A β25-35-induced neurotoxicity via PI3K/AKT signaling pathway in Alzheimer's disease.
CONCLUSION: All the data in this study revealed that miR-212 modulated PDCD4 to regulate cell proliferation, apoptosis through PI3K/AKT signaling pathway in Aβ25-35-treated SH-SY5Y and IMR-32 cells, and this new regulatory network may provide a novel mechanism of AD. PMID: 32474742 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 30, 2020 Category: Biotechnology Authors: Wang Y, Chang Q Tags: Biotechnol Lett Source Type: research

Marine bacterial community analysis on 316L stainless steel coupons by Illumina MiSeq sequencing.
acute;pio L Abstract In order to evaluate the corrosive action of microorganisms on 316L metal exposed directly to a marine environment, a system was designed to immerse coupons in seawater. After periods of 30, 60 and 90 days, the coupons were recovered, the corrosion rates evaluated and the biofilm samples on their surface were analyzed by 16S rRNA gene sequencing. The results of the corrosion rate showed an acceleration over the entire experimental period. Alpha diversity measurements showed higher rates after 60 days of the experiment, while abundance measurements showed higher rates after 90 da...
Source: Biotechnology Letters - May 29, 2020 Category: Biotechnology Authors: Capão A, Moreira-Filho P, Garcia M, Bitati S, Procópio L Tags: Biotechnol Lett Source Type: research

Proteomic analysis reveals the damaging role of low redox laccase from Yersinia enterocolitica strain 8081 in the midgut of Helicoverpa armigera.
CONCLUSIONS: All these data collectively suggest that H. armigera (Hübner) larvae can be used to study the effect of microbes and their metabolites on the host physiology, anatomy, and survival. PMID: 32472187 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 29, 2020 Category: Biotechnology Authors: Ahlawat S, Singh D, Yadav A, Singh AK, Virdi JS, Sharma KK Tags: Biotechnol Lett Source Type: research

Comparative evaluation of silver nanoparticles biosynthesis by two cold-tolerant Streptomyces strains and their biological activities.
In conclusion; although AgNPs produced by diverse strains of cold-adapted Streptomyces had close characteristics and biological activities, they showed some multifarious properties. PMID: 32462288 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 27, 2020 Category: Biotechnology Authors: Bakhtiari-Sardari A, Mashreghi M, Eshghi H, Behnam-Rasouli F, Lashani E, Shahnavaz B Tags: Biotechnol Lett Source Type: research

The synergy between LPMOs and cellulases in enzymatic saccharification of cellulose is both enzyme- and substrate-dependent.
CONCLUSIONS: The degree of synergy exhibited by the cellulase-LPMO mixtures was enzyme- and substrate-specific. The observed Cel7A inhibition, rather than synergy, by the C1-oxidizing LPMO, TtAA9E, warrants further investigations. PMID: 32458293 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 26, 2020 Category: Biotechnology Authors: Tokin R, Ipsen JØ, Westh P, Johansen KS Tags: Biotechnol Lett Source Type: research

Inhibition of Salmonella enteritidis biofilms by Salmonella invasion protein-targeting aptamer.
This study demonstrated quantitative and qualitative antibiofilm effect of Apt17 against the biofilms of two Salmonella enteritidis strains. According to our knowledge, this is the first study employing an aptamer that targets SipA protein to inhibit biofilm formation in Salmonella. PMID: 32451800 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 25, 2020 Category: Biotechnology Authors: Shatila F, Yaşa İ, Yalçın HT Tags: Biotechnol Lett Source Type: research

Mechanical loading induces HIF-1 α expression in chondrocytes via YAP.
CONCLUSION: Our results indicated that cyclic mechanical stress promoted HIF-1α stabilization and YAP is involved in mechanical stress induced HIF-1α up-regulation. PMID: 32451801 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 25, 2020 Category: Biotechnology Authors: Jing X, Yang X, Zhang W, Wang S, Cui X, Du T, Li T Tags: Biotechnol Lett Source Type: research

Single-nucleotide polymorphisms influence the promoter activities of systemic lupus erythematosus-associated genes.
Abstract Systemic lupus erythematosus (SLE) is a protypical autoimmune disease and genetic factors play important roles in its pathogenesis. Since present SLE susceptibility loci are mainly studied through meta-analysis of genome-wide association study, we performed promoter activity analysis to examine the biological functions of SLE-associated single-nucleotide polymorphisms (SNPs). We found at SNP positions rs1341239, rs1800795, rs1800796, rs1800872, rs1800871, rs187238, rs360719, rs8178822, rs3761549, different alleles influenced respective promoter activities in different manners, and the effects also appeare...
Source: Biotechnology Letters - May 25, 2020 Category: Biotechnology Authors: Qiu J, Qin X, Wen J, Wu L, Kong L, Ou Y, Yang S Tags: Biotechnol Lett Source Type: research

Optimization for allitol production from D-glucose by using immobilized glucose isomerase and recombinant E. coli expressing D-psicose-3-epimerase, ribitol dehydrogenase and formate dehydrogenase.
CONCLUSIONS: Allitol can be efficiently produced from low cost substrate D-glucose by using the method combining enzymatic catalysis and resting-cell biotransformation, which is the first report. PMID: 32451803 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 25, 2020 Category: Biotechnology Authors: Wen X, Lin H, Ren Y, Li C, Zhang C, Song X, Lin J, Lin J Tags: Biotechnol Lett Source Type: research

B1 protein: a novel cell penetrating protein for in vitro and in vivo delivery of HIV-1 multi-epitope DNA constructs.
CONCLUSION: Generally, our findings showed the potency of B1 protein as a promising gene delivery system to improve an effective therapeutic vaccine against HIV-1 infection. PMID: 32449070 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 25, 2020 Category: Biotechnology Authors: Kardani K, Bolhassani A, Agi E, Hashemi A Tags: Biotechnol Lett Source Type: research

Effect of a novel postbiotic containing lactic acid bacteria on the intestinal microbiota and disease resistance of rainbow trout (Oncorhynchus mykiss).
This study was aimed to assess the effect of a novel postbiotic on bacterial community composition and structure within the intestinal ecosystem of rainbow trout (Oncorhynchus mykiss), as well as evaluate its capacity to protect rainbow trout from Lactococcus garvieae infection. RESULTS: After 30 days of dietary postbiotic supplementation, high-throughput 16S rRNA gene sequencing revealed that bacterial community composition, diversity and richness were significantly higher in treated fish than in control fish. The proportion of sequences affiliated to the phylum Tenericutes, and to a lesser extent, the phyla Spirocha...
Source: Biotechnology Letters - May 25, 2020 Category: Biotechnology Authors: Mora-Sánchez B, Balcázar JL, Pérez-Sánchez T Tags: Biotechnol Lett Source Type: research

Internalization of the TAT-PBX1 fusion protein significantly enhances the proliferation of human hair follicle-derived mesenchymal stem cells and delays their senescence.
CONCLUSIONS: The TAT-PBX1 fusion protein increased the proliferation of hair follicle mesenchymal stem cells and delayed their senescence by activating the AKT pathway following internalization by cells. PMID: 32436118 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 20, 2020 Category: Biotechnology Authors: Wang B, Liu F, Liu Z, Han X, Lian A, Zhang Y, Zuo K, Wang Y, Liu M, Zou F, Jiang Y, Jin M, Liu X, Liu J Tags: Biotechnol Lett Source Type: research

MicroRNA-191 regulates differentiation and migration of mesenchymal stem cells and their paracrine effect on angiogenesis.
Abstract MicroRNAs (miRNAs) are critical regulators in organ development. Among them, miR-191 is known to be regulated in early embryogenesis and dysregulated in cancer. This role in undifferentiated tissues suggests a possible part of miR-191 also in bone marrow derived mesenchymal stem cells (BMSCs) physiology. Here, we report that miR-191 decreased MMP expression and migration of BMSCs. Conditioned media of miR-191 overexpressing BMSCs block VEGF expression, and inhibit angiogenesis of HUVECs. Under osteogenic culture conditions, inhibition of miR-191 significantly induces bone formation. Moreover, our studies ...
Source: Biotechnology Letters - May 20, 2020 Category: Biotechnology Authors: Liu Y, Liu X, Ye P, Zhang X, Schilling AF, Yonezawa T, Gao G, Cui X Tags: Biotechnol Lett Source Type: research

The effect of exosomes derived from mesenchymal stem cells in the treatment of induced type 1 diabetes mellitus in rats.
Abstract AIM: The aim of the current study was to evaluate the therapeutic and regenerative effects of MSCs derived exosomes in the treatment of type 1 DM and to compare its effects with MSCs themselves. The experiment was done on forty albino rats grouped as follows, group (1): Ten healthy rats, group (2): Ten induced type 1 DM rats, group (3): Ten induced type 1 DM rats received exosomes intraperitoneally, and group (4): Ten induced type 1 DM rats received MSCs intraperitoneally. Serum glucose and plasma insulin levels were assessed weekly. QRT-PCR was done to assess regeneration of pancreatic beta cells by meas...
Source: Biotechnology Letters - May 19, 2020 Category: Biotechnology Authors: Sabry D, Marzouk S, Zakaria R, Ibrahim HA, Samir M Tags: Biotechnol Lett Source Type: research

Small design from big alignment: engineering proteins with multiple sequence alignment as the starting point.
Abstract Multiple sequence alignment (MSA) is a fundamental way to gain information that cannot be obtained from the analysis of any individual sequence included in the alignment. It provides ways to investigate the relationship between sequence and function from a perspective of evolution. Thus, the MSA of proteins can be employed as a reference for protein engineering. In this paper, we reviewed the recent advances to highlight how protein engineering was benefited from the MSA of proteins. These methods include (1) engineering the thermostability or solubility of proteins by making it closer to the consensus se...
Source: Biotechnology Letters - May 19, 2020 Category: Biotechnology Authors: Wang T, Liang C, Hou Y, Zheng M, Xu H, An Y, Xiao S, Liu L, Lian S Tags: Biotechnol Lett Source Type: research

Enhanced production of Aspergillus niger inulinase from sugar beet molasses and its kinetic modeling.
CONCLUSIONS: The enzyme activity was increased by optimizing the concentration of the components used. It was demonstrated that the proposed kinetic models can victoriously define fungal development, enzyme fabrication, and sugar depletion. PMID: 32424732 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 18, 2020 Category: Biotechnology Authors: Germec M, Turhan I Tags: Biotechnol Lett Source Type: research

Soybean peptides promote yoghurt fermentation and quality.
CONCLUSIONS: In our study, the addition of soybean peptides (0.2%) can be effective both in maintaining the viable bacterial count and yoghurt quality. PMID: 32419046 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 17, 2020 Category: Biotechnology Authors: Hu Y, Li Y, Liu X Tags: Biotechnol Lett Source Type: research

Recombinant expression of sericin-cecropin fusion protein and its functional activity.
CONCLUSIONS: To our knowledge, this is the first report not only on the recombinant expression of sericin as a fusion protein but also the bactericidal possibility of the 38-amino acid serine-rich motif of sericin protein. We also discuss the potential biomedical and biotechnological applications of this sericin hybrid protein. PMID: 32418030 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 16, 2020 Category: Biotechnology Authors: Thomas DS, Manoharan C, Rasalkar S, Mishra RK, Gopalapillai R Tags: Biotechnol Lett Source Type: research

Characterization of the hydrolysate and catalytic cavity of α-agarase AgaD.
CONCLUSION: This study confirms AgaD is an endo-type α-agarase and A4 as the main hydrolysis product of AgaD, which suggests the catalytic cavity of AgaD accommodates eight sugar units spanning from - 4 to + 4. PMID: 32399660 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 13, 2020 Category: Biotechnology Authors: Wang H, Zhang W, Cui Z, Lu Z, Lu X Tags: Biotechnol Lett Source Type: research

The immune response to a recombinant Lactococcus lactis oral vaccine against foot-and-mouth disease virus in mice.
CONCLUSIONS: Potent immune responses were successfully elicited in mice with FMDV VP1 delivered through L. lactis. PMID: 32385744 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 9, 2020 Category: Biotechnology Authors: Liu X, Qi L, Lv J, Zhang Z, Zhou P, Ma Z, Wang Y, Zhang Y, Pan L Tags: Biotechnol Lett Source Type: research

PPAR α plays an important role in the migration activity, and the expression of CYP2S1 and CYP1B1 in chrysin-treated HCT116 cells.
CONCLUSION: The study indicates that PPARα may play an essential role in regulating the migration activity, and the expression of CYP2S1 and CYP1B1 in chrysin-treated colorectal cancer cells. PMID: 32385743 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 8, 2020 Category: Biotechnology Authors: Khor CY, Khoo BY Tags: Biotechnol Lett Source Type: research

MicroRNA-127-3p regulates myoblast proliferation by targeting Sept7.
CONCLUSIONS: These findings reveal that miR-127-3p regulates myoblast proliferation by targeting Sept7. PMID: 32382971 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 7, 2020 Category: Biotechnology Authors: Li J, Wang G, Jiang J, Zhang L, Zhou P, Ren H Tags: Biotechnol Lett Source Type: research

Construction of a system for single-stranded DNA isolation.
CONCLUSIONS: The ST-SSB system has obvious advantages of high efficiency and one-step purification to recycle any ssDNA. PMID: 32372267 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 5, 2020 Category: Biotechnology Authors: Hao M, Huang H, Hu Y, Qi H Tags: Biotechnol Lett Source Type: research

Functional variations between Mesenchymal Stem Cells of different tissue origins: A comparative gene expression profiling.
Abstract BACKGROUND: Mesenchymal Stem Cells (MSCs), regardless of the tissue sources, are considered as excellent candidates for cellular therapy as they are immune-privileged cells containing a multitude of therapeutic functions that aid in tissue regeneration and repair. For the effective application of these cells in cell therapy, it is important to understand and characterize their biological functions. OBJECTIVES: The present study attempts to characterize the variations in multipotent function such as cell surface antigen levels, proliferation, differentiation and stemness (pluripotency) potential of MS...
Source: Biotechnology Letters - May 5, 2020 Category: Biotechnology Authors: Sangeetha KN, Vennila R, Secunda R, Sakthivel S, Pathak S, Jeswanth S, Surendran R Tags: Biotechnol Lett Source Type: research

Biotransformation of androst-4-ene-3,17-dione and nandrolone decanoate by genera of Aspergillus and Fusarium.
Abstract The ability of five fungal species belonging to two genera of Aspergillus and Fusarium has been examined in the microbial transformation of androst-4-ene-3, 17-dione (AD). Furthermore, the biotransformation of nandrolone decanoate (2) by F. fujikuroi has been studied. AD (1) was converted by cultures of Aspergillus sp. PTCC 5266 to form 11α-hydroxy-AD (3) as the only product, with a yield of 86% in 3 days. Moreover, two hydroxylated metabolites 11α-hydroxy-AD (3, 65%) and 7β-hydroxy-AD (4; 18%) were isolated in biotransformation of AD by A. nidulans. On the other hand, it was metabol...
Source: Biotechnology Letters - May 2, 2020 Category: Biotechnology Authors: Heidary M, Ghasemi S, Habibi Z, Ansari F Tags: Biotechnol Lett Source Type: research

Mechanical injury and IL-1 β regulated LOXs and MMP-1, 2, 3 expression in ACL fibroblasts co-cultured with synoviocytes.
CONCLUSIONS: SCs can either inhibit or increase LOX production in the presence of IL-1β, while promoting the accumulation of MMP in injured ACLfs. These results may provide crucial insights into the mechanisms underlying ACL poor healing capacity after injury. PMID: 32358726 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 1, 2020 Category: Biotechnology Authors: Wang C, Chi Q, Sha Y, Xu K, Xu C, Chen C, Huang W, Chen P, Chen P, Yang L, Sung KLP Tags: Biotechnol Lett Source Type: research

Strong alkaline condition is preferable for producing 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa KNU-2B.
In this study, we report the production of 7,10-dihydroxy-8(E)-hexadecenoic acid (DHD) from palmitoleic acid by a new strain, Pseudomonas aeruginosa KNU-2B. KNU-2B efficiently produced DHD from palmitoleic acid and required a strong alkaline condition for maximum DHD production. The maximum DHD amount produced under pH 10.0 and 48-h incubation at 27 °C and 150 rpm was 219.5 mg/100 mL culture. Other important nutritional factors were also investigated to obtain optimum DHD production. PMID: 32356012 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - April 30, 2020 Category: Biotechnology Authors: Moon JS, Lee YJ, Kim IH, Kim HR Tags: Biotechnol Lett Source Type: research