Recycling selectable markers via Cre/loxP system for constructing Komagataella phaffii strains co-expressing multiple proteins
CONCLUSIONS: With easy manipulation, the method was effective in recycling of the selectable markers, and consequently two protein genes were sequential integrated chromosomally and successfully co-expressed in the yeast.PMID:38416308 | DOI:10.1007/s10529-024-03466-3 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 28, 2024 Category: Biotechnology Authors: Weixian Wang Minghai Han Guofei Zhu Xiaohui Liu Tianming Zhao Xiaoyan Ma Xun Gong Cunbin Xu Source Type: research

ANCUT1, a novel thermoalkaline cutinase from Aspergillus nidulans and its application on hydroxycinnamic acids lipophilization
Biotechnol Lett. 2024 Feb 28. doi: 10.1007/s10529-024-03467-2. Online ahead of print.ABSTRACTOne of the four cutinases encoded in the Aspergillus nidulans genome, ANCUT1, is described here. Culture conditions were evaluated, and it was found that this enzyme is produced only when cutin is present in the culture medium, unlike the previously described ANCUT2, with which it shares 62% amino acid identity. The differences between them include the fact that ANCUT1 is a smaller enzyme, with experimental molecular weight and pI values of 22 kDa and 6, respectively. It shows maximum activity at pH 9 and 60 °C under assayed condi...
Source: Biotechnology Letters - February 28, 2024 Category: Biotechnology Authors: Carolina Pe ña-Montes Eva Berm údez-García Denise Castro-Ochoa Fernanda Vega-P érez Katia Esqueda-Dom ínguez Jos é Augusto Castro-Rodríguez Augusto Gonz ález-Canto Laura Segoviano-Reyes Arturo Navarro-Oca ña Amelia Farr és Source Type: research

Recycling selectable markers via Cre/loxP system for constructing Komagataella phaffii strains co-expressing multiple proteins
CONCLUSIONS: With easy manipulation, the method was effective in recycling of the selectable markers, and consequently two protein genes were sequential integrated chromosomally and successfully co-expressed in the yeast.PMID:38416308 | DOI:10.1007/s10529-024-03466-3 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 28, 2024 Category: Biotechnology Authors: Weixian Wang Minghai Han Guofei Zhu Xiaohui Liu Tianming Zhao Xiaoyan Ma Xun Gong Cunbin Xu Source Type: research

ANCUT1, a novel thermoalkaline cutinase from Aspergillus nidulans and its application on hydroxycinnamic acids lipophilization
Biotechnol Lett. 2024 Feb 28. doi: 10.1007/s10529-024-03467-2. Online ahead of print.ABSTRACTOne of the four cutinases encoded in the Aspergillus nidulans genome, ANCUT1, is described here. Culture conditions were evaluated, and it was found that this enzyme is produced only when cutin is present in the culture medium, unlike the previously described ANCUT2, with which it shares 62% amino acid identity. The differences between them include the fact that ANCUT1 is a smaller enzyme, with experimental molecular weight and pI values of 22 kDa and 6, respectively. It shows maximum activity at pH 9 and 60 °C under assayed condi...
Source: Biotechnology Letters - February 28, 2024 Category: Biotechnology Authors: Carolina Pe ña-Montes Eva Berm údez-García Denise Castro-Ochoa Fernanda Vega-P érez Katia Esqueda-Dom ínguez Jos é Augusto Castro-Rodríguez Augusto Gonz ález-Canto Laura Segoviano-Reyes Arturo Navarro-Oca ña Amelia Farr és Source Type: research

In silico designing of novel epitope-based peptide vaccines against HIV-1
In this study, we explored the impacts of CD40L and IFN-γ as immunostimulatory adjuvants for our candidate HIV-1 Nef vaccine in human and mouse using immunoinformatics analyses. Overall, 18 IFN-γ-based vaccine constructs (9 constructs in human and 9 constructs in mouse), and 18 CD40L-based vaccine constructs (9 constructs in human and 9 constructs in mouse) were designed. To find immunogenic epitopes, important characteristics of each component (e.g., MHC-I and MHC-II binding, and peptide-MHC-I/MHC-II molecular docking) were determined. Then, the selected epitopes were applied to create multiepitope constructs. Finally, ...
Source: Biotechnology Letters - February 25, 2024 Category: Biotechnology Authors: Fatemeh Heidarnejad Ali Namvar Seyed Mehdi Sadat Parisa Moradi Pordanjani Fatemeh Rezaei Haideh Namdari Sina Arjmand Azam Bolhassani Source Type: research

In silico designing of novel epitope-based peptide vaccines against HIV-1
In this study, we explored the impacts of CD40L and IFN-γ as immunostimulatory adjuvants for our candidate HIV-1 Nef vaccine in human and mouse using immunoinformatics analyses. Overall, 18 IFN-γ-based vaccine constructs (9 constructs in human and 9 constructs in mouse), and 18 CD40L-based vaccine constructs (9 constructs in human and 9 constructs in mouse) were designed. To find immunogenic epitopes, important characteristics of each component (e.g., MHC-I and MHC-II binding, and peptide-MHC-I/MHC-II molecular docking) were determined. Then, the selected epitopes were applied to create multiepitope constructs. Finally, ...
Source: Biotechnology Letters - February 25, 2024 Category: Biotechnology Authors: Fatemeh Heidarnejad Ali Namvar Seyed Mehdi Sadat Parisa Moradi Pordanjani Fatemeh Rezaei Haideh Namdari Sina Arjmand Azam Bolhassani Source Type: research

In silico designing of novel epitope-based peptide vaccines against HIV-1
In this study, we explored the impacts of CD40L and IFN-γ as immunostimulatory adjuvants for our candidate HIV-1 Nef vaccine in human and mouse using immunoinformatics analyses. Overall, 18 IFN-γ-based vaccine constructs (9 constructs in human and 9 constructs in mouse), and 18 CD40L-based vaccine constructs (9 constructs in human and 9 constructs in mouse) were designed. To find immunogenic epitopes, important characteristics of each component (e.g., MHC-I and MHC-II binding, and peptide-MHC-I/MHC-II molecular docking) were determined. Then, the selected epitopes were applied to create multiepitope constructs. Finally, ...
Source: Biotechnology Letters - February 25, 2024 Category: Biotechnology Authors: Fatemeh Heidarnejad Ali Namvar Seyed Mehdi Sadat Parisa Moradi Pordanjani Fatemeh Rezaei Haideh Namdari Sina Arjmand Azam Bolhassani Source Type: research

Silica nanoparticles enhance interfacial self-adherence of a multi-layered extracellular matrix scaffold for vascular tissue regeneration
CONCLUSION: Our study demonstrated that SiNP prevents the unraveling of a multilayered extracellular matrix graft while improving the scaffolds' overall mechanical properties. In addition to the generation of a robust biomaterial for vascular tissue regeneration, this novel layering technology is a promising strategy for a number of bioengineering applications.PMID:38368285 | DOI:10.1007/s10529-024-03469-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 17, 2024 Category: Biotechnology Authors: Leslie A Goldberg Helena D Zomer Calum McFetridge Peter S McFetridge Source Type: research

Silica nanoparticles enhance interfacial self-adherence of a multi-layered extracellular matrix scaffold for vascular tissue regeneration
CONCLUSION: Our study demonstrated that SiNP prevents the unraveling of a multilayered extracellular matrix graft while improving the scaffolds' overall mechanical properties. In addition to the generation of a robust biomaterial for vascular tissue regeneration, this novel layering technology is a promising strategy for a number of bioengineering applications.PMID:38368285 | DOI:10.1007/s10529-024-03469-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 17, 2024 Category: Biotechnology Authors: Leslie A Goldberg Helena D Zomer Calum McFetridge Peter S McFetridge Source Type: research

Silica nanoparticles enhance interfacial self-adherence of a multi-layered extracellular matrix scaffold for vascular tissue regeneration
CONCLUSION: Our study demonstrated that SiNP prevents the unraveling of a multilayered extracellular matrix graft while improving the scaffolds' overall mechanical properties. In addition to the generation of a robust biomaterial for vascular tissue regeneration, this novel layering technology is a promising strategy for a number of bioengineering applications.PMID:38368285 | DOI:10.1007/s10529-024-03469-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 17, 2024 Category: Biotechnology Authors: Leslie A Goldberg Helena D Zomer Calum McFetridge Peter S McFetridge Source Type: research

Silica nanoparticles enhance interfacial self-adherence of a multi-layered extracellular matrix scaffold for vascular tissue regeneration
CONCLUSION: Our study demonstrated that SiNP prevents the unraveling of a multilayered extracellular matrix graft while improving the scaffolds' overall mechanical properties. In addition to the generation of a robust biomaterial for vascular tissue regeneration, this novel layering technology is a promising strategy for a number of bioengineering applications.PMID:38368285 | DOI:10.1007/s10529-024-03469-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 17, 2024 Category: Biotechnology Authors: Leslie A Goldberg Helena D Zomer Calum McFetridge Peter S McFetridge Source Type: research

Silica nanoparticles enhance interfacial self-adherence of a multi-layered extracellular matrix scaffold for vascular tissue regeneration
CONCLUSION: Our study demonstrated that SiNP prevents the unraveling of a multilayered extracellular matrix graft while improving the scaffolds' overall mechanical properties. In addition to the generation of a robust biomaterial for vascular tissue regeneration, this novel layering technology is a promising strategy for a number of bioengineering applications.PMID:38368285 | DOI:10.1007/s10529-024-03469-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 17, 2024 Category: Biotechnology Authors: Leslie A Goldberg Helena D Zomer Calum McFetridge Peter S McFetridge Source Type: research

Silica nanoparticles enhance interfacial self-adherence of a multi-layered extracellular matrix scaffold for vascular tissue regeneration
CONCLUSION: Our study demonstrated that SiNP prevents the unraveling of a multilayered extracellular matrix graft while improving the scaffolds' overall mechanical properties. In addition to the generation of a robust biomaterial for vascular tissue regeneration, this novel layering technology is a promising strategy for a number of bioengineering applications.PMID:38368285 | DOI:10.1007/s10529-024-03469-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 17, 2024 Category: Biotechnology Authors: Leslie A Goldberg Helena D Zomer Calum McFetridge Peter S McFetridge Source Type: research

Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system
CONCLUSION: The production of MSC-EVs in a 3D bioreactor system under hypoxic conditions resulted in increased EV concentration and purity. This system could be especially useful in screening culture conditions for the production of 3D-derived MSC-EVs.PMID:38349512 | DOI:10.1007/s10529-024-03465-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 13, 2024 Category: Biotechnology Authors: Ciarra Almeria Ren é Weiss Maike Keck Viktoria Weber Cornelia Kasper Dominik Egger Source Type: research

Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system
CONCLUSION: The production of MSC-EVs in a 3D bioreactor system under hypoxic conditions resulted in increased EV concentration and purity. This system could be especially useful in screening culture conditions for the production of 3D-derived MSC-EVs.PMID:38349512 | DOI:10.1007/s10529-024-03465-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 13, 2024 Category: Biotechnology Authors: Ciarra Almeria Ren é Weiss Maike Keck Viktoria Weber Cornelia Kasper Dominik Egger Source Type: research