A novel recombinant protein vaccine containing the different E7 proteins of the HPV16, 18, 6, 11 E7 linked to the HIV-1 Tat (47-57) improve cytotoxic immune responses
CONCLUSION: Our finding suggested that this novel fusion protein vaccine was able to induce therapeutic efficacy and immunogenicity by improving CD8 + T cell in TC-1 tumor bearing mice; so this vaccine may be appreciated for research against HPV and tumor immunotherapies.PMID:34313864 | DOI:10.1007/s10529-021-03166-2 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 27, 2021 Category: Biotechnology Authors: Tahoora Mousavi Reza Valadan Alireza Rafiei Ali Abbasi Mohammad Reza Haghshenas Source Type: research

A novel recombinant protein vaccine containing the different E7 proteins of the HPV16, 18, 6, 11 E7 linked to the HIV-1 Tat (47-57) improve cytotoxic immune responses
CONCLUSION: Our finding suggested that this novel fusion protein vaccine was able to induce therapeutic efficacy and immunogenicity by improving CD8 + T cell in TC-1 tumor bearing mice; so this vaccine may be appreciated for research against HPV and tumor immunotherapies.PMID:34313864 | DOI:10.1007/s10529-021-03166-2 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 27, 2021 Category: Biotechnology Authors: Tahoora Mousavi Reza Valadan Alireza Rafiei Ali Abbasi Mohammad Reza Haghshenas Source Type: research

A novel recombinant protein vaccine containing the different E7 proteins of the HPV16, 18, 6, 11 E7 linked to the HIV-1 Tat (47-57) improve cytotoxic immune responses
CONCLUSION: Our finding suggested that this novel fusion protein vaccine was able to induce therapeutic efficacy and immunogenicity by improving CD8 + T cell in TC-1 tumor bearing mice; so this vaccine may be appreciated for research against HPV and tumor immunotherapies.PMID:34313864 | DOI:10.1007/s10529-021-03166-2 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 27, 2021 Category: Biotechnology Authors: Tahoora Mousavi Reza Valadan Alireza Rafiei Ali Abbasi Mohammad Reza Haghshenas Source Type: research

Food-grade γ-aminobutyric acid production by immobilized glutamate decarboxylase from Lactobacillus plantarum in rice vinegar and monosodium glutamate system
CONCLUSIONS: The method of production GABA by immobilized GadB microspheres mixed in the rice vinegar and MSG reaction system is introduced herein for the first time. Especially, the results obtained here meet the increased interest in the harnessing of biocatalyst to synthesize food-grade GABA.PMID:34308525 | DOI:10.1007/s10529-021-03164-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 26, 2021 Category: Biotechnology Authors: Li-Li Yao Jia-Ren Cao Chang-Jiang Lyu Fang-Fang Fan Hong-Peng Wang Hong-Wei Cao Jun Huang Le-He Mei Source Type: research

Food-grade γ-aminobutyric acid production by immobilized glutamate decarboxylase from Lactobacillus plantarum in rice vinegar and monosodium glutamate system
CONCLUSIONS: The method of production GABA by immobilized GadB microspheres mixed in the rice vinegar and MSG reaction system is introduced herein for the first time. Especially, the results obtained here meet the increased interest in the harnessing of biocatalyst to synthesize food-grade GABA.PMID:34308525 | DOI:10.1007/s10529-021-03164-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 26, 2021 Category: Biotechnology Authors: Li-Li Yao Jia-Ren Cao Chang-Jiang Lyu Fang-Fang Fan Hong-Peng Wang Hong-Wei Cao Jun Huang Le-He Mei Source Type: research

Production of flavonol and flavone 6-C-glucosides by bioconversion in Escherichia coli expressing a C-glucosyltransferase from wasabi (Eutrema japonicum)
CONCLUSIONS: The Ec-WjGT1 system quickly and easily produces flavone and flavonol 6-C-glucosides at high conversion rates when using sequential administration to avoid precipitation of substrates.PMID:34302563 | DOI:10.1007/s10529-021-03165-3 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 24, 2021 Category: Biotechnology Authors: Nasanjargal Dorjjugder Mayu Hatano Goro Taguchi Source Type: research

Heterologous expression and biochemical characterization of a cold-active lipase from Rhizopus microsporus suitable for oleate synthesis and bread making
CONCLUSIONS: A novel cold-active lipase gene from R. microsporus was identified, and its application potentials were evaluated. RmLipA should be a potential candidate in oleates synthesis and bread making industries.PMID:34302564 | DOI:10.1007/s10529-021-03167-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 24, 2021 Category: Biotechnology Authors: Man Xiang Ling Wang Qiaojuan Yan Zhengqiang Jiang Shaoqing Yang Source Type: research

Production of flavonol and flavone 6-C-glucosides by bioconversion in Escherichia coli expressing a C-glucosyltransferase from wasabi (Eutrema japonicum)
CONCLUSIONS: The Ec-WjGT1 system quickly and easily produces flavone and flavonol 6-C-glucosides at high conversion rates when using sequential administration to avoid precipitation of substrates.PMID:34302563 | DOI:10.1007/s10529-021-03165-3 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 24, 2021 Category: Biotechnology Authors: Nasanjargal Dorjjugder Mayu Hatano Goro Taguchi Source Type: research

Heterologous expression and biochemical characterization of a cold-active lipase from Rhizopus microsporus suitable for oleate synthesis and bread making
CONCLUSIONS: A novel cold-active lipase gene from R. microsporus was identified, and its application potentials were evaluated. RmLipA should be a potential candidate in oleates synthesis and bread making industries.PMID:34302564 | DOI:10.1007/s10529-021-03167-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 24, 2021 Category: Biotechnology Authors: Man Xiang Ling Wang Qiaojuan Yan Zhengqiang Jiang Shaoqing Yang Source Type: research

Fabrication of gelatin microspheres containing ammonium hydrogen carbonate for the tunable release of herbicide
In this study, gelatin microspheres were prepared by emulsion and cross-linking, then they were loaded with 2,4-dichlorophenoxyacetic acid sodium (2,4-D Na) as a model herbicide. To achieve temperature-tunable release of 2,4-D Na from the microspheres, NH4HCO3 was added to the formulations at different concentrations. The prepared formulations were characterized by SEM, FTIR, and size distribution analyzes, and their drug loading capacities were determined. Based on bioassay experiments, the 2,4-D Na-NH4HCO3-loaded gelatin microspheres can effectively control the spread of dicotyledonous weeds. Therefore, the strategy prop...
Source: Biotechnology Letters - July 18, 2021 Category: Biotechnology Authors: He Liu Zheng Zhang Jiaxin Li Wanyu Zang Qing Yang Jun Yang Source Type: research

Fabrication of gelatin microspheres containing ammonium hydrogen carbonate for the tunable release of herbicide
In this study, gelatin microspheres were prepared by emulsion and cross-linking, then they were loaded with 2,4-dichlorophenoxyacetic acid sodium (2,4-D Na) as a model herbicide. To achieve temperature-tunable release of 2,4-D Na from the microspheres, NH4HCO3 was added to the formulations at different concentrations. The prepared formulations were characterized by SEM, FTIR, and size distribution analyzes, and their drug loading capacities were determined. Based on bioassay experiments, the 2,4-D Na-NH4HCO3-loaded gelatin microspheres can effectively control the spread of dicotyledonous weeds. Therefore, the strategy prop...
Source: Biotechnology Letters - July 18, 2021 Category: Biotechnology Authors: He Liu Zheng Zhang Jiaxin Li Wanyu Zang Qing Yang Jun Yang Source Type: research

Fabrication of gelatin microspheres containing ammonium hydrogen carbonate for the tunable release of herbicide
In this study, gelatin microspheres were prepared by emulsion and cross-linking, then they were loaded with 2,4-dichlorophenoxyacetic acid sodium (2,4-D Na) as a model herbicide. To achieve temperature-tunable release of 2,4-D Na from the microspheres, NH4HCO3 was added to the formulations at different concentrations. The prepared formulations were characterized by SEM, FTIR, and size distribution analyzes, and their drug loading capacities were determined. Based on bioassay experiments, the 2,4-D Na-NH4HCO3-loaded gelatin microspheres can effectively control the spread of dicotyledonous weeds. Therefore, the strategy prop...
Source: Biotechnology Letters - July 18, 2021 Category: Biotechnology Authors: He Liu Zheng Zhang Jiaxin Li Wanyu Zang Qing Yang Jun Yang Source Type: research

Fabrication of gelatin microspheres containing ammonium hydrogen carbonate for the tunable release of herbicide
In this study, gelatin microspheres were prepared by emulsion and cross-linking, then they were loaded with 2,4-dichlorophenoxyacetic acid sodium (2,4-D Na) as a model herbicide. To achieve temperature-tunable release of 2,4-D Na from the microspheres, NH4HCO3 was added to the formulations at different concentrations. The prepared formulations were characterized by SEM, FTIR, and size distribution analyzes, and their drug loading capacities were determined. Based on bioassay experiments, the 2,4-D Na-NH4HCO3-loaded gelatin microspheres can effectively control the spread of dicotyledonous weeds. Therefore, the strategy prop...
Source: Biotechnology Letters - July 18, 2021 Category: Biotechnology Authors: He Liu Zheng Zhang Jiaxin Li Wanyu Zang Qing Yang Jun Yang Source Type: research

Fabrication of gelatin microspheres containing ammonium hydrogen carbonate for the tunable release of herbicide
In this study, gelatin microspheres were prepared by emulsion and cross-linking, then they were loaded with 2,4-dichlorophenoxyacetic acid sodium (2,4-D Na) as a model herbicide. To achieve temperature-tunable release of 2,4-D Na from the microspheres, NH4HCO3 was added to the formulations at different concentrations. The prepared formulations were characterized by SEM, FTIR, and size distribution analyzes, and their drug loading capacities were determined. Based on bioassay experiments, the 2,4-D Na-NH4HCO3-loaded gelatin microspheres can effectively control the spread of dicotyledonous weeds. Therefore, the strategy prop...
Source: Biotechnology Letters - July 18, 2021 Category: Biotechnology Authors: He Liu Zheng Zhang Jiaxin Li Wanyu Zang Qing Yang Jun Yang Source Type: research

Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae
CONCLUSION: This work demonstrated for the first time that various immunocomplex structures of our target protein could be efficiently produced in S. cerevisiae for future application in developing oral and injectable vaccines against various pathogens.PMID:34245387 | PMC:PMC8272446 | DOI:10.1007/s10529-021-03161-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 10, 2021 Category: Biotechnology Authors: Kum-Kang So Jeesun Chun Nguyen Ngoc Luong Hee-Won Seo Dae-Hyuk Kim Source Type: research

Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae
CONCLUSION: This work demonstrated for the first time that various immunocomplex structures of our target protein could be efficiently produced in S. cerevisiae for future application in developing oral and injectable vaccines against various pathogens.PMID:34245387 | PMC:PMC8272446 | DOI:10.1007/s10529-021-03161-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 10, 2021 Category: Biotechnology Authors: Kum-Kang So Jeesun Chun Nguyen Ngoc Luong Hee-Won Seo Dae-Hyuk Kim Source Type: research

Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae
CONCLUSION: This work demonstrated for the first time that various immunocomplex structures of our target protein could be efficiently produced in S. cerevisiae for future application in developing oral and injectable vaccines against various pathogens.PMID:34245387 | PMC:PMC8272446 | DOI:10.1007/s10529-021-03161-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 10, 2021 Category: Biotechnology Authors: Kum-Kang So Jeesun Chun Nguyen Ngoc Luong Hee-Won Seo Dae-Hyuk Kim Source Type: research

Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae
CONCLUSION: This work demonstrated for the first time that various immunocomplex structures of our target protein could be efficiently produced in S. cerevisiae for future application in developing oral and injectable vaccines against various pathogens.PMID:34245387 | PMC:PMC8272446 | DOI:10.1007/s10529-021-03161-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 10, 2021 Category: Biotechnology Authors: Kum-Kang So Jeesun Chun Nguyen Ngoc Luong Hee-Won Seo Dae-Hyuk Kim Source Type: research

Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae
CONCLUSION: This work demonstrated for the first time that various immunocomplex structures of our target protein could be efficiently produced in S. cerevisiae for future application in developing oral and injectable vaccines against various pathogens.PMID:34245387 | PMC:PMC8272446 | DOI:10.1007/s10529-021-03161-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 10, 2021 Category: Biotechnology Authors: Kum-Kang So Jeesun Chun Nguyen Ngoc Luong Hee-Won Seo Dae-Hyuk Kim Source Type: research

Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae
CONCLUSION: This work demonstrated for the first time that various immunocomplex structures of our target protein could be efficiently produced in S. cerevisiae for future application in developing oral and injectable vaccines against various pathogens.PMID:34245387 | DOI:10.1007/s10529-021-03161-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 10, 2021 Category: Biotechnology Authors: Kum-Kang So Jeesun Chun Nguyen Ngoc Luong Hee-Won Seo Dae-Hyuk Kim Source Type: research

Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae
CONCLUSION: This work demonstrated for the first time that various immunocomplex structures of our target protein could be efficiently produced in S. cerevisiae for future application in developing oral and injectable vaccines against various pathogens.PMID:34245387 | DOI:10.1007/s10529-021-03161-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 10, 2021 Category: Biotechnology Authors: Kum-Kang So Jeesun Chun Nguyen Ngoc Luong Hee-Won Seo Dae-Hyuk Kim Source Type: research

Biosensors for the detection of organophosphate exposure by a new diethyl thiophosphate-specific aptamer
CONCLUSIONS: The current method showed great promise in using the DETP-specific aptamer to detect the exposure history to organophosphates by measuring their metabolites, although degradation of organophosphate parent compounds might occur.PMID:34231090 | DOI:10.1007/s10529-021-03158-2 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 7, 2021 Category: Biotechnology Authors: Napachanok Mongkoldhumrongkul Swainson Pongsakorn Aiemderm Chonnikarn Saikaew Kanyanat Theeraraksakul Pakjira Rimdusit Charoenkwan Kraiya Sasimanas Unajak Kiattawee Choowongkomon Source Type: research

Biosensors for the detection of organophosphate exposure by a new diethyl thiophosphate-specific aptamer
CONCLUSIONS: The current method showed great promise in using the DETP-specific aptamer to detect the exposure history to organophosphates by measuring their metabolites, although degradation of organophosphate parent compounds might occur.PMID:34231090 | DOI:10.1007/s10529-021-03158-2 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 7, 2021 Category: Biotechnology Authors: Napachanok Mongkoldhumrongkul Swainson Pongsakorn Aiemderm Chonnikarn Saikaew Kanyanat Theeraraksakul Pakjira Rimdusit Charoenkwan Kraiya Sasimanas Unajak Kiattawee Choowongkomon Source Type: research

Biosensors for the detection of organophosphate exposure by a new diethyl thiophosphate-specific aptamer
CONCLUSIONS: The current method showed great promise in using the DETP-specific aptamer to detect the exposure history to organophosphates by measuring their metabolites, although degradation of organophosphate parent compounds might occur.PMID:34231090 | DOI:10.1007/s10529-021-03158-2 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 7, 2021 Category: Biotechnology Authors: Napachanok Mongkoldhumrongkul Swainson Pongsakorn Aiemderm Chonnikarn Saikaew Kanyanat Theeraraksakul Pakjira Rimdusit Charoenkwan Kraiya Sasimanas Unajak Kiattawee Choowongkomon Source Type: research

Simple stain-free screening method for pectinolytic microorganisms under alkalophilic conditions
CONCLUSIONS: The developed method is useful for identifying pectin-degrading microorganisms.PMID:34228234 | DOI:10.1007/s10529-021-03162-6 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 6, 2021 Category: Biotechnology Authors: Mai Ishihara Masahiro Kikkawa Kazunori Shimizu Hiroaki Suzuki Hiroyuki Honda Source Type: research

Metabolic pathway analysis of walnut endophytic bacterium Bacillus subtilis HB1310 related to lipid production from fermentation of cotton stalk hydrolysate based on genome sequencing
CONCLUSIONS: This study is the first attempt to explain the metabolic mechanism of mixed sugar utilization and lipid production based on genomic information, which provides a theoretical basis for the metabolic regulation of bacterial lipid production from lignocellulosic hydrolysates.PMID:34228235 | DOI:10.1007/s10529-021-03160-8 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 6, 2021 Category: Biotechnology Authors: Qin Zhang Panpan Liu Yanbin Li Hui Jiang Source Type: research

Simple stain-free screening method for pectinolytic microorganisms under alkalophilic conditions
CONCLUSIONS: The developed method is useful for identifying pectin-degrading microorganisms.PMID:34228234 | DOI:10.1007/s10529-021-03162-6 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 6, 2021 Category: Biotechnology Authors: Mai Ishihara Masahiro Kikkawa Kazunori Shimizu Hiroaki Suzuki Hiroyuki Honda Source Type: research

Metabolic pathway analysis of walnut endophytic bacterium Bacillus subtilis HB1310 related to lipid production from fermentation of cotton stalk hydrolysate based on genome sequencing
CONCLUSIONS: This study is the first attempt to explain the metabolic mechanism of mixed sugar utilization and lipid production based on genomic information, which provides a theoretical basis for the metabolic regulation of bacterial lipid production from lignocellulosic hydrolysates.PMID:34228235 | DOI:10.1007/s10529-021-03160-8 (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 6, 2021 Category: Biotechnology Authors: Qin Zhang Panpan Liu Yanbin Li Hui Jiang Source Type: research

Application of the modified cytosine base-editing in the cultured cells of bama minipig
This study provides a foundation for further application of somatic cell cloning to construct MSTN-edited Bama minipigs that carry only a single-base mutation and avoids biosafety risks to a large extent, thereby providing experience and a reference for the base editing of other genetic loci in Bama minipigs.PMID:34189671 | DOI:10.1007/s10529-021-03159-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 30, 2021 Category: Biotechnology Authors: Jia-Sheng Pan Zi-Sheng Lin Jian-Cong Wen Jian-Feng Guo Xia-Hui Wu Ying-Ying Liu Wen-Jun Lai Qi-Ying Liang Yong-Shi Xie Yi-Rou Chen Yi-Hong Chen Ai-Fen Yan Juan Feng Lian Liu Dao-Yuan Gong Xiang-Xing Zhu Jia-Hong Lu Dong-Sheng Tang Source Type: research

Application of the modified cytosine base-editing in the cultured cells of bama minipig
This study provides a foundation for further application of somatic cell cloning to construct MSTN-edited Bama minipigs that carry only a single-base mutation and avoids biosafety risks to a large extent, thereby providing experience and a reference for the base editing of other genetic loci in Bama minipigs.PMID:34189671 | DOI:10.1007/s10529-021-03159-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 30, 2021 Category: Biotechnology Authors: Jia-Sheng Pan Zi-Sheng Lin Jian-Cong Wen Jian-Feng Guo Xia-Hui Wu Ying-Ying Liu Wen-Jun Lai Qi-Ying Liang Yong-Shi Xie Yi-Rou Chen Yi-Hong Chen Ai-Fen Yan Juan Feng Lian Liu Dao-Yuan Gong Xiang-Xing Zhu Jia-Hong Lu Dong-Sheng Tang Source Type: research

Application of the modified cytosine base-editing in the cultured cells of bama minipig
This study provides a foundation for further application of somatic cell cloning to construct MSTN-edited Bama minipigs that carry only a single-base mutation and avoids biosafety risks to a large extent, thereby providing experience and a reference for the base editing of other genetic loci in Bama minipigs.PMID:34189671 | DOI:10.1007/s10529-021-03159-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 30, 2021 Category: Biotechnology Authors: Jia-Sheng Pan Zi-Sheng Lin Jian-Cong Wen Jian-Feng Guo Xia-Hui Wu Ying-Ying Liu Wen-Jun Lai Qi-Ying Liang Yong-Shi Xie Yi-Rou Chen Yi-Hong Chen Ai-Fen Yan Juan Feng Lian Liu Dao-Yuan Gong Xiang-Xing Zhu Jia-Hong Lu Dong-Sheng Tang Source Type: research

Application of the modified cytosine base-editing in the cultured cells of bama minipig
This study provides a foundation for further application of somatic cell cloning to construct MSTN-edited Bama minipigs that carry only a single-base mutation and avoids biosafety risks to a large extent, thereby providing experience and a reference for the base editing of other genetic loci in Bama minipigs.PMID:34189671 | DOI:10.1007/s10529-021-03159-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 30, 2021 Category: Biotechnology Authors: Jia-Sheng Pan Zi-Sheng Lin Jian-Cong Wen Jian-Feng Guo Xia-Hui Wu Ying-Ying Liu Wen-Jun Lai Qi-Ying Liang Yong-Shi Xie Yi-Rou Chen Yi-Hong Chen Ai-Fen Yan Juan Feng Lian Liu Dao-Yuan Gong Xiang-Xing Zhu Jia-Hong Lu Dong-Sheng Tang Source Type: research

Application of the modified cytosine base-editing in the cultured cells of bama minipig
This study provides a foundation for further application of somatic cell cloning to construct MSTN-edited Bama minipigs that carry only a single-base mutation and avoids biosafety risks to a large extent, thereby providing experience and a reference for the base editing of other genetic loci in Bama minipigs.PMID:34189671 | DOI:10.1007/s10529-021-03159-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 30, 2021 Category: Biotechnology Authors: Jia-Sheng Pan Zi-Sheng Lin Jian-Cong Wen Jian-Feng Guo Xia-Hui Wu Ying-Ying Liu Wen-Jun Lai Qi-Ying Liang Yong-Shi Xie Yi-Rou Chen Yi-Hong Chen Ai-Fen Yan Juan Feng Lian Liu Dao-Yuan Gong Xiang-Xing Zhu Jia-Hong Lu Dong-Sheng Tang Source Type: research

A novel vector-based RNAi method using mouse U6 promoter-driven shRNA expression in the filamentous fungus Blakeslea trispora
CONCLUSIONS: The results suggest that the mouse U6 promoter that drives the expression of shRNA vectors may serve as a novel tool for RNAi induction in filamentous fungi.PMID:34185215 | DOI:10.1007/s10529-021-03155-5 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 29, 2021 Category: Biotechnology Authors: Ye Li Hui Feng Lihua Jin Xiulan Xin Qipeng Yuan Source Type: research

A novel vector-based RNAi method using mouse U6 promoter-driven shRNA expression in the filamentous fungus Blakeslea trispora
CONCLUSIONS: The results suggest that the mouse U6 promoter that drives the expression of shRNA vectors may serve as a novel tool for RNAi induction in filamentous fungi.PMID:34185215 | DOI:10.1007/s10529-021-03155-5 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 29, 2021 Category: Biotechnology Authors: Ye Li Hui Feng Lihua Jin Xiulan Xin Qipeng Yuan Source Type: research

The construction of a dual-functional strain that produces both polysaccharides and sulfotransferases
CONCLUSIONS: These results indicate that EcN::T7M is capable of preparing sulfotransferase and heparosan at the same time. The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.PMID:34176028 | DOI:10.1007/s10529-021-03156-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 27, 2021 Category: Biotechnology Authors: Xiaomei Li Yanying Yu Jiaqing Tang Bingxue Gong Wenjing Li Tingting Chen Xianxuan Zhou Source Type: research

The construction of a dual-functional strain that produces both polysaccharides and sulfotransferases
CONCLUSIONS: These results indicate that EcN::T7M is capable of preparing sulfotransferase and heparosan at the same time. The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.PMID:34176028 | DOI:10.1007/s10529-021-03156-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 27, 2021 Category: Biotechnology Authors: Xiaomei Li Yanying Yu Jiaqing Tang Bingxue Gong Wenjing Li Tingting Chen Xianxuan Zhou Source Type: research

Comparison of different protocols of RNA preparation from circulating blood for RNA sequencing
CONCLUSIONS: The quality of RNA extracted from circulating blood is affected by high-speed centrifugation and cryopreservation. Adding an RNA stabilizer during the cryopreservation of circulating blood significantly improved RNA quality and quantity. The quality of extracted RNA from circulating blood is improved when using TRIzol relative to that attained with a commercial kit.PMID:34173130 | DOI:10.1007/s10529-021-03152-8 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 26, 2021 Category: Biotechnology Authors: Shenghua Li Lan Chen Jinpin Li Jingli Liu Source Type: research

Comparison of different protocols of RNA preparation from circulating blood for RNA sequencing
CONCLUSIONS: The quality of RNA extracted from circulating blood is affected by high-speed centrifugation and cryopreservation. Adding an RNA stabilizer during the cryopreservation of circulating blood significantly improved RNA quality and quantity. The quality of extracted RNA from circulating blood is improved when using TRIzol relative to that attained with a commercial kit.PMID:34173130 | DOI:10.1007/s10529-021-03152-8 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 26, 2021 Category: Biotechnology Authors: Shenghua Li Lan Chen Jinpin Li Jingli Liu Source Type: research

Infected insect gut reveals differentially expressed proteins for cellular redox, metal resistance and secretion system in Yersinia enterocolitica-Helicoverpa armigera pathogenic model
CONCLUSIONS: All data from current study collectively suggest that H. armigera larva can be considered as a potential in vivo model system for studying the enteropathogenic infection by Y. enterocolitica strain 8081.PMID:34165641 | DOI:10.1007/s10529-021-03157-3 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 24, 2021 Category: Biotechnology Authors: Shruti Ahlawat Amarjeet Kumar Singh Akshay Shankar Asha Yadav Krishna Kant Sharma Source Type: research

Infected insect gut reveals differentially expressed proteins for cellular redox, metal resistance and secretion system in Yersinia enterocolitica-Helicoverpa armigera pathogenic model
CONCLUSIONS: All data from current study collectively suggest that H. armigera larva can be considered as a potential in vivo model system for studying the enteropathogenic infection by Y. enterocolitica strain 8081.PMID:34165641 | DOI:10.1007/s10529-021-03157-3 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 24, 2021 Category: Biotechnology Authors: Shruti Ahlawat Amarjeet Kumar Singh Akshay Shankar Asha Yadav Krishna Kant Sharma Source Type: research

Combination of atmospheric and room temperature plasma (ARTP) mutagenesis, genome shuffling and dimethyl sulfoxide (DMSO) feeding to improve FK506 production in Streptomyces tsukubaensis
Biotechnol Lett. 2021 Jun 23. doi: 10.1007/s10529-021-03154-6. Online ahead of print.ABSTRACTFK506 is a clinically important macrocyclic polyketide with immunosuppressive activity produced by Streptomyces tsukubaensis. However, the production capacity of the strain is very low. To improve production, atmospheric and room temperature plasma (ARTP) mutagenesis was adopted to get the initial strains used in genome shuffling (GS). After three rounds of GS, S. tsukubaensis R3-C4 was the most productive strain, resulting in a FK506 concentration of 335 μg/mL, 2.6 times than that of the original wild-type strain. Moreover, exo...
Source: Biotechnology Letters - June 23, 2021 Category: Biotechnology Authors: Liting Ye Ruifang Ye Fengxian Hu Guozhu Wang Source Type: research

Enhanced β-carotene production by overexpressing the DID2 gene, a subunit of ESCRT complex, in engineered Yarrowia lipolytica
CONCLUSION: The ESCRT protein complex subunit, DID2, improved β-carotene production in engineered Y. lipolytica and beneficial to glucose utilization and cofactors consumption. This study provided new finding of the DID2 gene's function and it mostly could be used for many other natural product productions.PMID:34160748 | DOI:10.1007/s10529-021-03150-w (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 23, 2021 Category: Biotechnology Authors: Fan Yang Liang Liu Shan Qiang Ching Yuan Hu Ying Li Yong Hong Meng Source Type: research

Combination of atmospheric and room temperature plasma (ARTP) mutagenesis, genome shuffling and dimethyl sulfoxide (DMSO) feeding to improve FK506 production in Streptomyces tsukubaensis
Biotechnol Lett. 2021 Jun 23. doi: 10.1007/s10529-021-03154-6. Online ahead of print.ABSTRACTFK506 is a clinically important macrocyclic polyketide with immunosuppressive activity produced by Streptomyces tsukubaensis. However, the production capacity of the strain is very low. To improve production, atmospheric and room temperature plasma (ARTP) mutagenesis was adopted to get the initial strains used in genome shuffling (GS). After three rounds of GS, S. tsukubaensis R3-C4 was the most productive strain, resulting in a FK506 concentration of 335 μg/mL, 2.6 times than that of the original wild-type strain. Moreover, exo...
Source: Biotechnology Letters - June 23, 2021 Category: Biotechnology Authors: Liting Ye Ruifang Ye Fengxian Hu Guozhu Wang Source Type: research

Enhanced β-carotene production by overexpressing the DID2 gene, a subunit of ESCRT complex, in engineered Yarrowia lipolytica
CONCLUSION: The ESCRT protein complex subunit, DID2, improved β-carotene production in engineered Y. lipolytica and beneficial to glucose utilization and cofactors consumption. This study provided new finding of the DID2 gene's function and it mostly could be used for many other natural product productions.PMID:34160748 | DOI:10.1007/s10529-021-03150-w (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 23, 2021 Category: Biotechnology Authors: Fan Yang Liang Liu Shan Qiang Ching Yuan Hu Ying Li Yong Hong Meng Source Type: research

Differential expression of miRNAs and functional role of mir-200a in high and low productivity CHO cells expressing an Fc fusion protein
CONCLUSION: These results may suggest a role for miR-200a in post-transcriptional regulation of the UPR, presenting miR-200a as a potential target for engineering industrially attractive CHO cell phenotypes.PMID:34131805 | DOI:10.1007/s10529-021-03153-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 16, 2021 Category: Biotechnology Authors: Laura Bryan Michael Henry Niall Barron Clair Gallagher Ronan M Kelly Christopher C Frye Matthew D Osborne Martin Clynes Paula Meleady Source Type: research

Differential expression of miRNAs and functional role of mir-200a in high and low productivity CHO cells expressing an Fc fusion protein
CONCLUSION: These results may suggest a role for miR-200a in post-transcriptional regulation of the UPR, presenting miR-200a as a potential target for engineering industrially attractive CHO cell phenotypes.PMID:34131805 | DOI:10.1007/s10529-021-03153-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 16, 2021 Category: Biotechnology Authors: Laura Bryan Michael Henry Niall Barron Clair Gallagher Ronan M Kelly Christopher C Frye Matthew D Osborne Martin Clynes Paula Meleady Source Type: research

Differential expression of miRNAs and functional role of mir-200a in high and low productivity CHO cells expressing an Fc fusion protein
CONCLUSION: These results may suggest a role for miR-200a in post-transcriptional regulation of the UPR, presenting miR-200a as a potential target for engineering industrially attractive CHO cell phenotypes.PMID:34131805 | DOI:10.1007/s10529-021-03153-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 16, 2021 Category: Biotechnology Authors: Laura Bryan Michael Henry Niall Barron Clair Gallagher Ronan M Kelly Christopher C Frye Matthew D Osborne Martin Clynes Paula Meleady Source Type: research

Differential expression of miRNAs and functional role of mir-200a in high and low productivity CHO cells expressing an Fc fusion protein
CONCLUSION: These results may suggest a role for miR-200a in post-transcriptional regulation of the UPR, presenting miR-200a as a potential target for engineering industrially attractive CHO cell phenotypes.PMID:34131805 | DOI:10.1007/s10529-021-03153-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 16, 2021 Category: Biotechnology Authors: Laura Bryan Michael Henry Niall Barron Clair Gallagher Ronan M Kelly Christopher C Frye Matthew D Osborne Martin Clynes Paula Meleady Source Type: research

Differential expression of miRNAs and functional role of mir-200a in high and low productivity CHO cells expressing an Fc fusion protein
CONCLUSION: These results may suggest a role for miR-200a in post-transcriptional regulation of the UPR, presenting miR-200a as a potential target for engineering industrially attractive CHO cell phenotypes.PMID:34131805 | DOI:10.1007/s10529-021-03153-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 16, 2021 Category: Biotechnology Authors: Laura Bryan Michael Henry Niall Barron Clair Gallagher Ronan M Kelly Christopher C Frye Matthew D Osborne Martin Clynes Paula Meleady Source Type: research