Soluble overexpression of a flagellin derivative from Salmonella enterica using synonymous codon substitutions of 5'-coding region in Escherichia coli.
CONCLUSION: Results showed that scvCBLB502-5, engineered through the synonymous codon substitutions, was easily expressed in functionally soluble form and maintained the proper folding to be recognized by TLR5, as an inducer for pathogen-associated molecular pattern (PAMP). PMID: 31535307 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - September 18, 2019 Category: Biotechnology Authors: Cheong DE, Lee J, Choi HJ, Yoo SK, Lee DH, Kim GJ Tags: Biotechnol Lett Source Type: research

Design and in vitro delivery of HIV-1 multi-epitope DNA and peptide constructs using novel cell-penetrating peptides.
Abstract OBJECTIVES: Developing an effective HIV vaccine that stimulates the humoral and cellular immune responses is still challenging because of the diversity of HIV-1 virus, polymorphism of human HLA and lack of a suitable delivery system. RESULTS: Using bioinformatics tools, we designed a DNA construct encoding multiple epitopes. These epitopes were highly conserved within prevalent HIV-1 subtypes and interacted with prevalent class I and II HLAs in Iran and the world. The designed DNA construct included Nef60-84, Nef126-144, Vpr34-47, Vpr60-75, Gp16030-53, Gp160308-323 and P248-151 epitopes (i.e., nef-vp...
Source: Biotechnology Letters - September 17, 2019 Category: Biotechnology Authors: Davoodi S, Bolhassani A, Sadat SM, Irani S Tags: Biotechnol Lett Source Type: research

A kinetic model to optimize and direct the dose ratio of Dsz enzymes in the 4S desulfurization pathway in vitro and in vivo.
CONCLUSIONS: According to the results predicted by the enzyme kinetic model, maintaining higher concentrations of DszC and DszB in the desulfurization system can effectively improve the desulfurization efficiency. PMID: 31522352 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - September 14, 2019 Category: Biotechnology Authors: Li L, Ye L, Guo Z, Zhang W, Liao X, Lin Y, Liang S Tags: Biotechnol Lett Source Type: research

Autochthonous lactic acid bacteria-presentation of potential probiotics application.
CONCLUSION: The results indicated the potential probiotic properties of the isolates and give evidence for further investigation and potential application in the dairy industry. PMID: 31515644 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - September 12, 2019 Category: Biotechnology Authors: Grujović MŽ, Mladenović KG, Nikodijević DD, Čomić LR Tags: Biotechnol Lett Source Type: research

Characterization of a glucose tolerant β-glucosidase from Aspergillus unguis with high potential as a blend-in for biomass hydrolyzing enzyme cocktails.
CONCLUSION: The GT-BGL from A. unguis NII 08123 was proven effective as a blend in for biomass hydrolyzing enzyme cocktails and the possible reasons for its glucose tolerance was determined through studies on its modeled structure. PMID: 31489522 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - September 5, 2019 Category: Biotechnology Authors: Kooloth Valappil P, Rajasree KP, Abraham A, Christopher M, Sukumaran RK Tags: Biotechnol Lett Source Type: research

Kinetics and thermodynamics of lipase catalysed synthesis of propyl caprate.
CONCLUSIONS: The results showed that the lipase-catalyzed esterification exhibits an ordered bi-bi mechanism with capric acid inhibiting the reaction and forming the dead-end complex with the lipase. Under the given set of reaction conditions, the lipase catalysed esterification reaction was anticipated to be spontaneous, referring to the value of the Gibbs free energy change (ΔG). Moreover, the esterification process was found to be endothermic, based on the values of enthalpy (ΔH) and entropy (ΔS). PMID: 31463715 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - August 28, 2019 Category: Biotechnology Authors: Parikh DT, Lanjekar KJ, Rathod VK Tags: Biotechnol Lett Source Type: research

Purification, identification and characterization of an esterase with high enantioselectivity to (S)-ethyl indoline-2-carboxylate.
CONCLUSIONS: In this study, a new esterase BaCE with an apparent molecular mass of 35 kDa was purified to homogeneity for the first time. The esterase from Bacillus aryabhattai B8W22 was isolated with a purification more than 59-fold and a yield of 20% by anion exchange chromatography and hydrophobic interaction chromatography. And its biochemical characterization were described in detail with pNPB as substrate. It displayed high enantioselectivity toward (S)-ethyl indoline-2-carboxylate. We next plan to highly express esterase BaCE in Escherichia coli, and apply it to industrial production of (S)-indoline-2-carboxyli...
Source: Biotechnology Letters - August 27, 2019 Category: Biotechnology Authors: Zhang YJ, Chen CS, Liu HT, Chen JL, Xia Y, Wu SJ Tags: Biotechnol Lett Source Type: research

The antibiotic activity and mechanisms of active metabolites (Streptomyces alboflavus TD-1) against Ralstonia solanacearum.
CONCLUSIONS: The active metabolites produced by S. alboflavus TD-1 liquid fermentation contain components that can significant inhibitory effects on R. solanacearum. It had the potential to develop biocontrol agents against bacterial wilt and be a kind potential sources for the preparation of functional anti-pathogenic microbial agents. PMID: 31451996 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - August 26, 2019 Category: Biotechnology Authors: Xue Y, Yang M, Li S, Li Z, Liu H, Guo Q, Wang C Tags: Biotechnol Lett Source Type: research

Ac25 in Autographa californica multiple nucleopolyhedrovirus was crucial for progeny budded virion production.
CONCLUSION: Ac25 is highly accumulated in the nucleus and promotes progeny virus production by stimulating viral genome replication and up-regulating the expression of late genes. Two potential applications of vAc-Ac25-EGFP were proposed: an improved bac-to-bac eukaryotic protein expression systems and biopesticides. PMID: 31444662 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - August 23, 2019 Category: Biotechnology Authors: Guo Y, Liang A, Fu Y Tags: Biotechnol Lett Source Type: research

Identification of internal control genes for circular RNAs.
CONCLUSIONS: In conclusion, since the stability of circRNAs is higher than that of linear RNAs, hsa_circ_0000284 and hsa_circ_0000471 may be used as reference genes not only for circRNAs but also for other kinds of RNAs. The findings in the present study fill the gap of lacking reference genes in the detection of circRNAs. PMID: 31428905 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - August 19, 2019 Category: Biotechnology Authors: Zhong S, Zhou S, Yang S, Yu X, Xu H, Wang J, Zhang Q, Lv M, Feng J Tags: Biotechnol Lett Source Type: research

Quorum-sensing based small RNA regulation for dynamic and tuneable gene expression.
CONCLUSIONS: We engineered bacterial small RNA to develop a pathway-independent strategy that can dynamically repress the expression of any gene at the posttranscription level. PMID: 31428906 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - August 19, 2019 Category: Biotechnology Authors: Bao SH, Li WY, Liu CJ, Zhang DY, Meng E Tags: Biotechnol Lett Source Type: research

Professor Christopher Julian Hewitt FREng 1969-2019.
Authors: PMID: 31420816 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - August 16, 2019 Category: Biotechnology Tags: Biotechnol Lett Source Type: research

Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2.
CONCLUSIONS: Alg17B can be used to saccharify the main carbohydrate, alginate, in the ethanolic production of brown algae fuel as well as in preparing and researching oligosaccharides. PMID: 31418101 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - August 16, 2019 Category: Biotechnology Authors: Huang G, Wen S, Liao S, Wang Q, Pan S, Zhang R, Lei F, Liao W, Feng J, Huang S Tags: Biotechnol Lett Source Type: research

Imaging aquatic animal cells and associated pathogens by atomic force microscopy in air.
In this study, we used AFM to image three different types of biological material from shrimp (Litopenaeus vannamei) in air, including hemocytes and two associated pathogens. Without obvious deformations when the cells were imaged in air and in the case for the haemocytes and the cells were fixed as well. The result suggests hydrophobic glass coverslips are a suitable substrate for adhesion of these samples. The method described here can be applied to the preparation of other fragile biological samples from aquatic animals for high-resolution analyses of host-pathogen interactions and other basic physiological processes. ...
Source: Biotechnology Letters - August 13, 2019 Category: Biotechnology Authors: Li C, Liang Y, Xu M, Wang H, Wang C, Huang J Tags: Biotechnol Lett Source Type: research

Characterization of a novel multi-domain xylanase from Clostridium clariflavum with application in hydrolysis of corn cobs.
CONCLUSIONS: Since the native multi-CDs' xylanases are rare, the thermostable Xyn2083 provides a good source for functional studies of two CDs coexisted in one xylanase and for potential applications after modification. PMID: 31410658 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - August 13, 2019 Category: Biotechnology Authors: Liu Y, Sun Y, Wang H, Tang L Tags: Biotechnol Lett Source Type: research

Overexpression of acetyl-CoA carboxylase increases fatty acid production in the green alga Chlamydomonas reinhardtii.
Abstract Chlamydomonas reinhardtii is a photosynthetic unicellular model algae with multiple biotechnological advantages, and its fatty acids can be used to produce biofuels. Numerous studies suggest that acetyl-coA carboxylase (ACCa) catalyzes the first committed and rate-limiting step of fatty acid biosynthesis, thereby playing a central role in oil accumulation. Here, we cloned and overexpressed ACCa in C. reinhardtii to directly evaluate its effect on fatty acid synthesis. GC-MS analysis found that the unsaturated FAs contents of the CW15-24 and CW15-85 strains were 55.45% and 56.15%, which were significantly ...
Source: Biotechnology Letters - August 9, 2019 Category: Biotechnology Authors: Chen D, Yuan X, Liang L, Liu K, Ye H, Liu Z, Liu Y, Huang L, He W, Chen Y, Zhang Y, Xue T Tags: Biotechnol Lett Source Type: research

A cofactor consumption screen identifies promising NfsB family nitroreductases for dinitrotoluene remediation.
CONCLUSIONS: Vibrio vulnificus NfsB is a particularly promising candidate for bioremediation applications, being ca. fivefold more catalytically efficient with 2,4-dinitrotoluene and over 26-fold more active with 2,6-dinitrotoluene than the benchmark E. coli nitroreductases NfsA and NfsB. PMID: 31392514 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - August 7, 2019 Category: Biotechnology Authors: Williams EM, Sharrock AV, Rylott EL, Bruce NC, MacKichan JK, Ackerley DF Tags: Biotechnol Lett Source Type: research

Glucoindole alkaloid accumulation induced by yeast extract in Uncaria tomentosa root cultures is involved in defense response.
CONCLUSION: The YE elicitor induced an antioxidant defense response, increased the transcript levels of genes encoding enzymes related to strictosidine biosynthesis precursors and class III peroxidases, and decreased the transcript level of SGD. Thus, YE could stimulate antifungal DHC production in root cultures of U. tomentosa. PMID: 31388801 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - August 6, 2019 Category: Biotechnology Authors: Correa-Higuera LJ, Sepúlveda-García EB, Ponce-Noyola T, Trejo-Espino JL, Jiménez-Aparicio AR, Luna-Palencia GR, Trejo-Tapia G, Ramos-Valdivia AC Tags: Biotechnol Lett Source Type: research

Towards a surrogate system to express human lipid binding TCRs.
CONCLUSIONS: The results suggest that the mis-pairing of the endogenous α/β TCR during ER folding in the presence of the new human TCR sequences could be impairing the functionality of the TCR lipid receptors. The surrogate systems presented here are important first steps in the establishment of human cell-specific lipid responsive libraries for the study of natural lipid substances. PMID: 31346817 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 26, 2019 Category: Biotechnology Authors: Wang R, Pscheid R, Ghumra A, Kan LYL, Cochrane S, Fairclough L, Alcocer MJC Tags: Biotechnol Lett Source Type: research

Multivariate analysis of metabolic parameters and optimization of antibody production using high cell density hybridoma in hollow fiber bioreactors.
CONCLUSION: The single-use HFBs could provide the advantages including high cell density, low shear stress, and continuous antibody production. PMID: 31325004 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 19, 2019 Category: Biotechnology Authors: Liu CH, Liu YX, Kumari M, Wu WC Tags: Biotechnol Lett Source Type: research

Optimized dual assay for the transgenes selection and screening in CHO cell line development for recombinant protein production.
CONCLUSIONS: Our method: (i) allows to include into workflow clones that demonstrate slow growth during single cell cloning but producing high amounts of a target protein, which otherwise would be lost in standard protocols of cells screening; (ii) can be applied for testing of DNA vectors for transfection and protein production; (iii) can be used for monitoring the heterogeneity of cell population and analysis of stable pools productivity. PMID: 31321593 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 18, 2019 Category: Biotechnology Authors: Beketova EV, Ibneeva LR, Abdulina YA, Dergousova EA, Filatov VL, Kozlovsky SV, Shilov ES, Datskevich PN, Rozov FN Tags: Biotechnol Lett Source Type: research

Identification and characterization of an acetyl esterase from Paenibacillus sp. XW-6-66 and its novel function in 7-aminocephalosporanic acid deacetylation.
CONCLUSIONS: Our results demonstrate the characterization of a new acetyl esterase belonging to the Aes family with potential biotechnological applications. PMID: 31302814 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 13, 2019 Category: Biotechnology Authors: Ding J, Zhou Y, Zhu H, Deng M, Long L, Yang Y, Wu Q, Huang Z Tags: Biotechnol Lett Source Type: research

An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast.
CONCLUSIONS: The method highlights the utility of the T2A-Leu2-based ribosomal skipping strategy for increasing the quality of the input library for selection, along with an optimized selection protocol based on thermo-resistant yeast cells. PMID: 31300936 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 12, 2019 Category: Biotechnology Authors: Jia Y, Ren P, Duan S, Zeng P, Xie D, Zeng F Tags: Biotechnol Lett Source Type: research

Preincubation with a low concentration of methyl- β-cyclodextrin enhances baculovirus expression system productivity.
CONCLUSIONS: Preincubation with a low concentration MβCD enhance baculovirus infection and foreign protein expression productivity. PMID: 31286325 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 8, 2019 Category: Biotechnology Authors: Huang J, Liu N, Shen X, Hao B Tags: Biotechnol Lett Source Type: research

Insights into the hydrolytic activity of Asclepias fruticosa L. protease.
CONCLUSIONS: Asclepain f was revealed as a successful enzyme for biocatalysis of protein hydrolysis processes at alkaline pH. This new plant protease has a broad substrate specificity and is capable of selectively degrading the fractions of soy proteins and improving its functional properties. PMID: 31286326 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 8, 2019 Category: Biotechnology Authors: Torres MJ, Natalucci C, López LMI, Trejo SA Tags: Biotechnol Lett Source Type: research

Purification and enzymatic characterization of the RNA ligase RTCB from Thermus thermophilus.
CONCLUSION: D64 was proved to be essential for RTCB-catalyzed RNA ligation and DNA activation (from 37 to 70 °C) in T. thermophilus. PMID: 31280403 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 6, 2019 Category: Biotechnology Authors: Duan S, Chen Z, Li Z, Ji R, Gan J, Li J Tags: Biotechnol Lett Source Type: research

Synthetic cell-cell communication in a three-species consortium for one-step vitamin C fermentation.
CONCLUSIONS: We successfully constructed a novel three-species consortia for one-step vitamin C fermentation by strengthening the cell-cell communication. This will be very useful for probing the rational design principles of more complex multi-microbial consortia. PMID: 31278569 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 5, 2019 Category: Biotechnology Authors: Wang EX, Liu Y, Ma Q, Dong XT, Ding MZ, Yuan YJ Tags: Biotechnol Lett Source Type: research

Enhancement of marinacarboline A biosynthesis by optimizing the riboflavin supplement in marine derived Nocardiopsis flavescens CGMCC 4.5723.
CONCLUSIONS: Optimization of riboflavin supplement could be a new promising strategy in actinomycetic marinacarboline A exploitation. PMID: 31270649 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - July 3, 2019 Category: Biotechnology Authors: Chen Q, Wu C, Zhang Y, Liu X, Chen J, Lei L, Xie Y Tags: Biotechnol Lett Source Type: research

Effective and economical column-based method for RNA isolation from animal cells.
CONCLUSIONS: The proposed RNA extraction method has the advantages of low toxicity, safe handling, and low cost. Isolation can be completed in 20 min. The proposed method can be used to extract RNA from various animal cell samples and is worth promoting. PMID: 31256272 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 29, 2019 Category: Biotechnology Authors: Niu Q, Cao M, Chen S, Zhao Y, Chen L, Li C, Zhou X Tags: Biotechnol Lett Source Type: research

Basic concepts, current evidence, and future potential for gene therapy in managing cutaneous wounds.
CONCLUSIONS: This article gives an overview of the methods and investigations applied on gene therapy in wound healing. However, clinical investigations need to be undertaken to gain a better understanding of gene delivery technologies and their roles in stimulating wound repair. PMID: 31256273 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 29, 2019 Category: Biotechnology Authors: Oryan A, Alemzadeh E, Zarei M Tags: Biotechnol Lett Source Type: research

Feasible development of stable HEK293 clones by CRISPR/Cas9-mediated site-specific integration for biopharmaceuticals production.
CONCLUSIONS: CRISPR/Cas9 mediated site-specific integration is an efficient and reliable tool to establishment recombinant stable HEK293 cell lines for both academic and industrial applications. PMID: 31236787 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 24, 2019 Category: Biotechnology Authors: Yang H, Wang J, Zhao M, Zhu J, Zhang M, Wang Z, Gao Y, Zhu W, Lu H Tags: Biotechnol Lett Source Type: research

Screening sediment samples used as anolytes in microbial fuel cells for microbial electron transfer activity using DREAM assay.
CONCLUSIONS: The DREAM assay is a rapid, sensitive and low-cost method to assess microbial electron transfer activity for inocula used as anolytes in a MFC. PMID: 31236788 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 24, 2019 Category: Biotechnology Authors: Aiyer KS, Vijayakumar BS Tags: Biotechnol Lett Source Type: research

Identification of cis-regulatory regions responsible for developmental and hormonal regulation of HbHMGS1 in transgenic Arabidopsis thaliana.
CONCLUSION: The - 454 bp HbHMGS1 promoter has great application potential in plant transformation studies as an alternative to the CaMV 35S promoter. The HbHMGS1 promoter may play important roles in regulating ethylene-, MeJA- and GA-mediated gene expression. The functional complexity of cis-elements revealed by this study remains to be elucidated. PMID: 31236789 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 24, 2019 Category: Biotechnology Authors: Gong XX, Yan BY, Tan YR, Gao X, Wang D, Zhang H, Wang P, Li SJ, Wang Y, Zhou LY, Liu JP Tags: Biotechnol Lett Source Type: research

Antibacterial mode of fibrauretine and synergistic effect with kanamycin against multi-drug resistant Escherichia coli.
CONCLUSIONS: Our results indicated that fibrauretine exerted synergistic effect with kanamycin and its antibacterial mode of action mainly attributed to disruption of cell membrane integrity. PMID: 31203568 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - June 15, 2019 Category: Biotechnology Authors: Wang YM, Kong LC, Liu L, Odah KA, Liu SM, Jiang XY, Ma HX Tags: Biotechnol Lett Source Type: research

Extracellular biosynthesis of silver nanoparticles from Penicillium italicum and its antioxidant, antimicrobial and cytotoxicity activities.
Abstract OBJECTIVES: The synthesis of silver nanoparticles and its antioxidant, antimicrobial and cytotoxic activities was investigated and extracellularly biosynthesized using Penicillium italicum isolated from Iraqi lemon fruits. RESULTS: The formation of synthesized nanoparticles was observed after 72 h of incubation. Color changing, ultraviolet and visible spectrum, X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive analysis X- ray confirmed the biosynthesis and characterization of silver nanoparticles. Ultr...
Source: Biotechnology Letters - June 14, 2019 Category: Biotechnology Authors: Taha ZK, Hawar SN, Sulaiman GM Tags: Biotechnol Lett Source Type: research

SpyTag/SpyCatcher cyclization enhances the thermostability and organic solvent tolerance of L-phenylalanine aldolase.
CONCLUSIONS: These results suggest that the in vivo cyclization using SpyTag/SpyCatcher is an effective strategy to improve the stability of enzymes, which potentially could be applied in industrial bioconversion. PMID: 31152342 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 31, 2019 Category: Biotechnology Authors: Wang Y, Tian J, Xiao Y, Wang Y, Sun H, Chang Y, Luo H Tags: Biotechnol Lett Source Type: research

Lactobacillus rhamnosus GG microcapsules inhibit Escherichia coli biofilm formation in coculture.
CONCLUSIONS: Lactobacillus rhamnosus GG microcapsules can effectively inhibit biofilm formation and disturb mature biofilms. PMID: 31147809 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 30, 2019 Category: Biotechnology Authors: Song H, Zhang J, Qu J, Liu J, Yin P, Zhang G, Shang D Tags: Biotechnol Lett Source Type: research

Degradation of the phenolic β-ether lignin model dimer and dyes by dye-decolorizing peroxidase from Bacillus amyloliquefaciens.
CONCLUSIONS: BaDyP was identified as a bacteria peroxidase with great potential for the degradation of lignin and bioremediation of dye-contamination. PMID: 31134460 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 27, 2019 Category: Biotechnology Authors: Yang J, Gao T, Zhang Y, Wang S, Li H, Li S, Wang S Tags: Biotechnol Lett Source Type: research

Recombinant lactococcus lactis secreting viral protein 1 of enterovirus 71 and its immunogenicity in mice.
Abstract OBJECTIVE: To construct recombinant Lactococcus lactis (L. lactis) expressing viral protein 1 (VP1) of enterovirus 71 (EV71) and evaluate its immunogenicity to be used as an oral vaccine in BALB/c mice. RESULTS: Recombinant L. lactis competent in secreting VP1 (~ 30 kDa) into the extracellular environment with the aid of the signal peptide Usp45 was produced. Enzyme-linked immunosorbent assay showed that significant VP1-specific antibody response including the production of both serum IgG and fecal IgA (p 
Source: Biotechnology Letters - May 22, 2019 Category: Biotechnology Authors: Xu P, Wang Y, Tao L, Wu X, Wu W Tags: Biotechnol Lett Source Type: research

Improved recombinant protein production by regulation of transcription and protein transport in Chinese hamster ovary cells.
CONCLUSIONS: We found that some genes involved in transcription and protein transport would improve recombinant protein production in CHO cells. PMID: 31114947 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 21, 2019 Category: Biotechnology Authors: Chen K, Li D, Li H, Li B, Wang R, Jiang L, Huang L, Xu X, Li J, Teng F, Jiang C, Gu H, Fang J Tags: Biotechnol Lett Source Type: research

Correction to: FabG: from a core to circumstantial catalyst.
Abstract In the original publication of the article, under section Polyketide synthesis: a pathway similar to fatty acid synthesis, the sentences "Phylogeny of KS domains and proteins of FAS and PKS, inferred by Bayesian estimation. Numbers above branches indicate posterior clade probability values." and "Branch length indicates number of inferred amino acid changes per position." in the first paragraph were included inadvertently. PMID: 31102074 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 17, 2019 Category: Biotechnology Authors: Shanbhag AP Tags: Biotechnol Lett Source Type: research

Noninvasive imaging of hepatocyte IL-6/STAT3 signaling pathway for evaluating inflammation responses induced by end-stage stored whole blood transfusion.
CONCLUSION: The hepatocyte STAT3 signaling pathway, which is activated by end-stage SWB transfusion, is associated with the elevation of systemic IL-6 secreted by macrophages. The STAT3-Luc mouse may serve as a mouse model for monitoring inflammation responses after end-stage SWB transfusion. PMID: 31102075 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 17, 2019 Category: Biotechnology Authors: Wang Z, Zhang Y, Zhou Q, Ma P, Wang X, Zhan L Tags: Biotechnol Lett Source Type: research

Molecular cloning, expression and characterization of a novel feruloyl esterase from a soil metagenomic library with phthalate-degrading activity.
CONCLUSION: The current investigation discovered a novel FAE with phthalate-degrading activity and highlighted the usefulness of metagenomic approaches as a powerful tool for discovery of novel FAEs. PMID: 31102076 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 17, 2019 Category: Biotechnology Authors: Wu S, Nan F, Jiang J, Qiu J, Zhang Y, Qiao B, Li S, Xin Z Tags: Biotechnol Lett Source Type: research

Activation of alternative metabolic pathways diverts carbon flux away from isobutanol formation in an engineered Escherichia coli strain.
CONCLUSIONS: These results demonstrate that the genetic modifications carried out led to activation of alternative pathways that diverted carbon flux toward formation of unwanted metabolites. The present study highlights how precursor metabolites can be metabolized through enzymatic routes that have not been considered important in previous studies due to the different strategies employed therein. The insights gained from the present study will allow rational genetic modification of host cells for production of metabolites of interest. PMID: 31093837 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 15, 2019 Category: Biotechnology Authors: Deb SS, Reshamwala SMS, Lali AM Tags: Biotechnol Lett Source Type: research

Pseudomonas monteilii PN1: a great potential P-nitrophenol degrader with plant growth promoting traits under drought and saline-alkali stresses.
CONCLUSIONS: Application of P. monteilii PN1 can be an extremely useful approach for the development of bioinoculants in improving plant tolerance to several abiotic stresses and removing PNP in soils to ensure secure crop production. PMID: 31089841 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 14, 2019 Category: Biotechnology Authors: Zhang Y, Yang F, Ren H, Liu J, Mu W, Wang Y Tags: Biotechnol Lett Source Type: research

GC-MS based profiling of alkanes in the filamentous yeast Hyphozyma roseoniger (Moesziomyces antarcticus).
CONCLUSIONS: The major hydrocarbons comprised linear and branched structures. The dominant alkanes were all odd- or even-carbon numbered long-chain n-alkanes, C15 > C18 > C24. PMID: 31079345 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 11, 2019 Category: Biotechnology Authors: Ncube EN, Steenkamp L, Dubery IA Tags: Biotechnol Lett Source Type: research

Multiphosphorylation and cellular localization of poly(rC) binding protein 1 during mitosis in hela cell.
CONCLUSIONS: These observations of mitosis-specific multiphosphorylations and localization shifts of PCBP1 suggest that the versatile PCBP1 was regulatable in a phosphorylation modification- and temporospatial-dependent manner in mitotic regulatory networks. PMID: 31076991 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 10, 2019 Category: Biotechnology Authors: Ma X, Li H, Cao X, Liu Z, He D Tags: Biotechnol Lett Source Type: research

MiR-499 inhibited hypoxia/reoxygenation induced cardiomyocytes injury by targeting SOX6.
CONCLUSION: We proved that miR-499-5p inhibited H/R-induced cardiomyocytes injury by targeting SOX6. Our results suggested that miR-499-5p/SOX6 pathway may present a potential therapeutic target for the treatment of AMI. PMID: 31076992 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 10, 2019 Category: Biotechnology Authors: Shi Y, Han Y, Niu L, Li J, Chen Y Tags: Biotechnol Lett Source Type: research

Pharmacological Notch pathway inhibition leads to cell cycle arrest and stimulates ascl1 and neurogenin2 genes expression in dental pulp stem cells-derived neurospheres.
CONCLUSIONS: Our findings demonstrated the Notch activity in hDPSCs-neurospheres. DAPT treatment positively regulated proneural genes expression and increased neuronal-like differentiation. PMID: 31073804 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 9, 2019 Category: Biotechnology Authors: Niapour A, Ghasemi Hamidabadi H, Niapour N, Mohammadi P, Sharifi Pasandi M, Malekzadeh V Tags: Biotechnol Lett Source Type: research

Precursor-feeding and altered-growth conditions reveal novel blue pigment production by Rubrivivax benzoatilyticus JA2.
CONCLUSION: Our approach unraveled the previously unknown pigment synthesis in strain JA2 and demonstrated the potential of mining strategy in discovering the hidden secondary metabolite repertoire in microorganisms. PMID: 31069568 [PubMed - as supplied by publisher] (Source: Biotechnology Letters)
Source: Biotechnology Letters - May 8, 2019 Category: Biotechnology Authors: Mekala LP, Mohammed M, Chintalapati S, Chintalapati VR Tags: Biotechnol Lett Source Type: research