The Novel Amidase PcnH Initiates the Degradation of Phenazine-1-Carboxamide in Sphingomonas histidinilytica DS-9
This study identified a novel amidase, PcnH, that catalyzed the hydrolysis of PCN to PCA. In addition, the PCA 1,2-dioxygenase PcaA1A2A3A4 and 2OHPC dioxygenase PcnD were also found to be involved in the subsequent degradation steps of PCN in S. histidinilytica DS-9. And the genes responsible for PCN catabolism are highly conserved in some strains of Sphingomonas. These results deepen our understanding of the PCN degradation mechanism.PMID:35579476 | DOI:10.1128/aem.00543-22 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - May 17, 2022 Category: Microbiology Authors: Yijun Ren Mingliang Zhang Siyuan Gao Qian Zhu Zhijian Ke Wankui Jiang Jiguo Qiu Qing Hong Source Type: research

Riemerella anatipestifer T9SS Effector SspA Functions in Bacterial Virulence and Defending Natural Host Immunity
In this study, a secreted subtilisin-like serine protease SspA was shown to be associated with R. anatipestifer virulence, host complement evasion, and degradation of gelatin, fibrinogen, and LL-37. The enzymatic activity of recombinant SspA was determined, and its Km and Vmax were 10.15 mM and 246.96 U/mg, respectively. Three conserved sites (Asp126, His158, and Ser410) are necessary for the protein's function. The median lethal dose of the sspA-deleted mutant strain was reduced >10,000-fold, indicating that SspA is an important virulence factor. In summary, we demonstrate that the R. anatipestifer AS87_RS04190 gene en...
Source: Applied and Environmental Microbiology - May 16, 2022 Category: Microbiology Authors: Zongchao Chen Pengfei Niu Xiaomei Ren Wenlong Han Ruyu Shen Min Zhu Yang Yu Chan Ding Shengqing Yu Source Type: research

A Novel Small RNA, DsrO, in Deinococcus radiodurans Promotes Methionine Sulfoxide Reductase ( < em > msrA < /em > ) Expression for Oxidative Stress Adaptation
This study indicates that protecting proteins from oxidation is an effective strategy for extremophiles to adapt to stress conditions.PMID:35575549 | DOI:10.1128/aem.00038-22 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - May 16, 2022 Category: Microbiology Authors: Yun Chen Mingming Zhao Mengli Lv Min Lin Jin Wang Kaijing Zuo Source Type: research

Genetic and Phenotypic Study of the < em > Pectobacterium versatile < /em > Beta-Lactamase, the Enzyme Most Similar to the Plasmid-Encoded TEM-1
This study aimed to compare the chromosomal beta-lactamase from Pectobacterium versatile, PEC-1, with the well-known and globally distributed TEM-1 in terms of genetic and functional properties. Despite the similarities between the enzymes, we obtained no definitive proof of gene transfer for the emergence of blaPEC-1 from blaTEM-1. Indeed, given the limited degree of sequence identity and the absence of a common genetic environment, it seems unlikely that any transfer of this gene has occurred recently. However, although blaPEC-1 was found mostly in one specific clade of the P. versatile species, certain isolates from oth...
Source: Applied and Environmental Microbiology - May 16, 2022 Category: Microbiology Authors: Guilhem Royer Zoya Dixit Jacques P édron Gautier Pierrat Vanessa Demontant B éatrice Berçot Christophe Rodriguez Marie-Anne Barny Herv é Jacquier Paul-Louis Woerther Source Type: research

Enhancing the Thermostability of Phytase to Boiling Point by Evolution-Guided Design
In this study, the phytase APPA from Yersinia intermedia was designed by evolution-guided design. Through the collection of homologous sequences in the NCBI database, we obtained a sequence set composed of 5,569 sequences, counted the number and locations of motif N-X-T/S, and selected the sites with high frequency in evolution as candidate sites for experiments. Based on the principle that N-glycosylation modification sites are located on the protein surface, 13 mutants were designed to optimize the number and location of N-glycosylation sites. Through experimental verification, 7 single mutants with improved thermostabil...
Source: Applied and Environmental Microbiology - May 13, 2022 Category: Microbiology Authors: Qian Wang Xiaoqing Liu Jian Tian Yaru Wang Honglian Zhang Yuan Wang Huiying Luo Bin Yao Huoqing Huang Tao Tu Source Type: research

Enhancing the Thermostability of Phytase to Boiling Point by Evolution-Guided Design
In this study, the phytase APPA from Yersinia intermedia was designed by evolution-guided design. Through the collection of homologous sequences in the NCBI database, we obtained a sequence set composed of 5,569 sequences, counted the number and locations of motif N-X-T/S, and selected the sites with high frequency in evolution as candidate sites for experiments. Based on the principle that N-glycosylation modification sites are located on the protein surface, 13 mutants were designed to optimize the number and location of N-glycosylation sites. Through experimental verification, 7 single mutants with improved thermostabil...
Source: Applied and Environmental Microbiology - May 13, 2022 Category: Microbiology Authors: Qian Wang Xiaoqing Liu Jian Tian Yaru Wang Honglian Zhang Yuan Wang Huiying Luo Bin Yao Huoqing Huang Tao Tu Source Type: research

Enhancing the Thermostability of Phytase to Boiling Point by Evolution-Guided Design
In this study, the phytase APPA from Yersinia intermedia was designed by evolution-guided design. Through the collection of homologous sequences in the NCBI database, we obtained a sequence set composed of 5,569 sequences, counted the number and locations of motif N-X-T/S, and selected the sites with high frequency in evolution as candidate sites for experiments. Based on the principle that N-glycosylation modification sites are located on the protein surface, 13 mutants were designed to optimize the number and location of N-glycosylation sites. Through experimental verification, 7 single mutants with improved thermostabil...
Source: Applied and Environmental Microbiology - May 13, 2022 Category: Microbiology Authors: Qian Wang Xiaoqing Liu Jian Tian Yaru Wang Honglian Zhang Yuan Wang Huiying Luo Bin Yao Huoqing Huang Tao Tu Source Type: research

Enhancing the Thermostability of Phytase to Boiling Point by Evolution-Guided Design
In this study, the phytase APPA from Yersinia intermedia was designed by evolution-guided design. Through the collection of homologous sequences in the NCBI database, we obtained a sequence set composed of 5,569 sequences, counted the number and locations of motif N-X-T/S, and selected the sites with high frequency in evolution as candidate sites for experiments. Based on the principle that N-glycosylation modification sites are located on the protein surface, 13 mutants were designed to optimize the number and location of N-glycosylation sites. Through experimental verification, 7 single mutants with improved thermostabil...
Source: Applied and Environmental Microbiology - May 13, 2022 Category: Microbiology Authors: Qian Wang Xiaoqing Liu Jian Tian Yaru Wang Honglian Zhang Yuan Wang Huiying Luo Bin Yao Huoqing Huang Tao Tu Source Type: research

Articles of Significant Interest in This Issue
Appl Environ Microbiol. 2022 May 10;88(9):e0072922. doi: 10.1128/aem.00729-22. Epub 2022 May 10.NO ABSTRACTPMID:35536026 | PMC:PMC9088269 | DOI:10.1128/aem.00729-22 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - May 10, 2022 Category: Microbiology Source Type: research

Variation in Root Exudate Composition Influences Soil Microbiome Membership and Function
Appl Environ Microbiol. 2022 May 10:e0022622. doi: 10.1128/aem.00226-22. Online ahead of print.ABSTRACTRoot exudation is one of the primary processes that mediate interactions between plant roots, microorganisms, and the soil matrix, yet the mechanisms by which exudation alters microbial metabolism in soils have been challenging to unravel. Here, utilizing distinct sorghum genotypes, we characterized the chemical heterogeneity between root exudates and the effects of that variability on soil microbial membership and metabolism. Distinct exudate chemical profiles were quantified and used to formulate synthetic root exudate ...
Source: Applied and Environmental Microbiology - May 10, 2022 Category: Microbiology Authors: Valerie A Seitz Bridget B McGivern Rebecca A Daly Jacqueline M Chaparro Mikayla A Borton Amy M Sheflin Stephen Kresovich Lindsay Shields Meagan E Schipanski Kelly C Wrighton Jessica E Prenni Source Type: research

Circulation of < em > Streptococcus agalactiae < /em > ST103 in a Free Stall Italian Dairy Farm
We report here on an outbreak of mastitis caused by Streptococcus agalactiae, or group B Streptococcus, in a northern Italy (Lombardy Region) free stall dairy farm. This outbreak was unusual because it occurred in a closed dairy herd and proved to be extremely difficult to resolve even after the application of the classical control procedures, which are specifically focused on the contagious nature of S. agalactiae. In order to better understand the potential origins of the pathogen and the critical points that could impair the eradication program and to investigate the possible presence of S. agalactiae in sources outside...
Source: Applied and Environmental Microbiology - May 10, 2022 Category: Microbiology Authors: Filippo Barsi Elena Carra Matteo Ricchi Gisella Gnali Giuliano Pisoni Simone Russo Anita Filippi Norma Arrigoni Ruth N Zadoks Chiara Garbarino Source Type: research

Articles of Significant Interest in This Issue
Appl Environ Microbiol. 2022 May 10;88(9):e0072922. doi: 10.1128/aem.00729-22. Epub 2022 May 10.NO ABSTRACTPMID:35536026 | DOI:10.1128/aem.00729-22 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - May 10, 2022 Category: Microbiology Source Type: research

Variation in Root Exudate Composition Influences Soil Microbiome Membership and Function
Appl Environ Microbiol. 2022 May 10:e0022622. doi: 10.1128/aem.00226-22. Online ahead of print.ABSTRACTRoot exudation is one of the primary processes that mediate interactions between plant roots, microorganisms, and the soil matrix, yet the mechanisms by which exudation alters microbial metabolism in soils have been challenging to unravel. Here, utilizing distinct sorghum genotypes, we characterized the chemical heterogeneity between root exudates and the effects of that variability on soil microbial membership and metabolism. Distinct exudate chemical profiles were quantified and used to formulate synthetic root exudate ...
Source: Applied and Environmental Microbiology - May 10, 2022 Category: Microbiology Authors: Valerie A Seitz Bridget B McGivern Rebecca A Daly Jacqueline M Chaparro Mikayla A Borton Amy M Sheflin Stephen Kresovich Lindsay Shields Meagan E Schipanski Kelly C Wrighton Jessica E Prenni Source Type: research

Circulation of < em > Streptococcus agalactiae < /em > ST103 in a Free Stall Italian Dairy Farm
We report here on an outbreak of mastitis caused by Streptococcus agalactiae, or group B Streptococcus, in a northern Italy (Lombardy Region) free stall dairy farm. This outbreak was unusual because it occurred in a closed dairy herd and proved to be extremely difficult to resolve even after the application of the classical control procedures, which are specifically focused on the contagious nature of S. agalactiae. In order to better understand the potential origins of the pathogen and the critical points that could impair the eradication program and to investigate the possible presence of S. agalactiae in sources outside...
Source: Applied and Environmental Microbiology - May 10, 2022 Category: Microbiology Authors: Filippo Barsi Elena Carra Matteo Ricchi Gisella Gnali Giuliano Pisoni Simone Russo Anita Filippi Norma Arrigoni Ruth N Zadoks Chiara Garbarino Source Type: research

Articles of Significant Interest in This Issue
Appl Environ Microbiol. 2022 May 10;88(9):e0072922. doi: 10.1128/aem.00729-22. Epub 2022 May 10.NO ABSTRACTPMID:35536026 | DOI:10.1128/aem.00729-22 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - May 10, 2022 Category: Microbiology Source Type: research