Applied and Environmental Microbiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Gut microbiota intervention alleviates pulmonary inflammation in broilers exposed to fine particulate matter from broiler house
In conclusion, gut microbiota could affect PM2.5-induced lung inflammation in broilers by adjusting the capacity of antigen-presenting cells to activate T-cell differentiation.IMPORTANCE: Gut microbes can influence the development of lung inflammation, and fine particulate matter collected from broiler houses can lead to lung inflammation in broilers. In this study, we explored the effect of gut microbes modified by intestinal non-absorbable antibiotics on particulate matter-induced lung inflammation. The results showed that modification in the composition of gut microbiota could alleviate lung inflammation by attenuating ...
Source: Applied and Environmental Microbiology - April 24, 2024 Category: Microbiology Authors: Junze Liu Yuan Li Dan Shen Xiaoqing Li Kai Wang Kentaro Nagaoka Chunmei Li Source Type: research

An overview of Parafrankia (Nod+/Fix+) and Pseudofrankia (Nod+/Fix-) interactions through genome mining and experimental modeling in co-culture and co-inoculation of Elaeagnus angustifolia 
This study delves into the ecological interactions between Parafrankia EUN1f and Pseudofrankia inefficax EuI1c, isolated from Elaeagnus umbellata nodules. EUN1f (Nod+/Fix+) and EuI1c (Nod+/Fix-) display contrasting symbiotic traits. While the prediction suggests a competitive scenario, the absence of direct interaction evidence implies that the competitive advantage of EUN1f and EuI1c is likely contingent on contextual factors such as substrate availability and the specific nature of stressors in their respective habitats. In co-culture, EUN1f outperforms EuI1c, especially under specific conditions, driven by its nitrogena...
Source: Applied and Environmental Microbiology - April 23, 2024 Category: Microbiology Authors: Maher Gtari Nicholas J Beauchemin Indrani Sarker Arnab Sen Faten Ghodhbane-Gtari Louis S Tisa Source Type: research

Azole resistance mechanisms and population structure of the human pathogen Aspergillus fumigatus on retail plant products
Appl Environ Microbiol. 2024 Apr 23:e0205623. doi: 10.1128/aem.02056-23. Online ahead of print.ABSTRACTAspergillus fumigatus is a ubiquitous saprotroph and human-pathogenic fungus that is life-threatening to the immunocompromised. Triazole-resistant A. fumigatus was found in patients without prior treatment with azoles, leading researchers to conclude that resistance had developed in agricultural environments where azoles are used against plant pathogens. Previous studies have documented azole-resistant A. fumigatus across agricultural environments, but few have looked at retail plant products. Our objectives were to deter...
Source: Applied and Environmental Microbiology - April 23, 2024 Category: Microbiology Authors: Caroline Wang Natalie Miller Douglas Vines Paul M Severns Michelle Momany Marin T Brewer Source Type: research

Reduction of product composition variability using pooled microbiome ecosystem therapy and consequence in two infectious murine models
In this study, we compared the metagenomic profile of pooled products to corresponding single donor-derived products. We demonstrated that pooled products are more homogeneous, diverse, and enriched in beneficial bacteria known to produce anti-inflammatory short chain fatty acids compared to single donor-derived products. We then evaluated pooled products' efficacy compared to corresponding single donor-derived products in Salmonella and C. difficile infectious mouse models. We were able to demonstrate that pooled products decreased pathogenicity by inducing a structural change in the intestinal microbiota composition. Sin...
Source: Applied and Environmental Microbiology - April 23, 2024 Category: Microbiology Authors: Julie Reygner Johanne Delannoy Marie-Th érèse Barba-Goudiaby Cyrielle Gasc Beno ît Levast Enora Gaschet Laurent Ferraris St éphane Paul Nathalie Kapel Anne-Judith Waligora-Dupriet Frederic Barbut Muriel Thomas Carole Schwintner Bastien Laperrousaz Nat Source Type: research

Erratum for Konstanti et al., "Physiology of γ-aminobutyric acid production by Akkermansia muciniphila "
Appl Environ Microbiol. 2024 Apr 23:e0049824. doi: 10.1128/aem.00498-24. Online ahead of print.NO ABSTRACTPMID:38651931 | DOI:10.1128/aem.00498-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - April 23, 2024 Category: Microbiology Authors: Prokopis Konstanti Kate Ligthart Christos Fryganas Constantinos Patinios Hauke Smidt Willem M de Vos Clara Belzer Source Type: research

Impact of operational conditions on drinking water biofilm dynamics and coliform invasion potential
In this study, we investigated whether indicator organisms for drinking water quality, such as coliforms, can settle in mature drinking water biofilms. Therefore, a biofilm monitor consisting of glass rings was used to grow and sample drinking water biofilms. Two mature drinking water biofilms were characterized by flow cytometry, ATP measurements, confocal laser scanning microscopy, and 16S rRNA sequencing. Biofilms developed under treated chlorinated surface water supply exhibited lower cell densities in comparison with biofilms resulting from treated groundwater. Overall, the phenotypic as well as the genotypic characte...
Source: Applied and Environmental Microbiology - April 22, 2024 Category: Microbiology Authors: Fien Waegenaar Cristina Garc ía-Timermans Josefien Van Landuyt Bart De Gusseme Nico Boon Source Type: research

Microbial contamination pathways in a poultry abattoir provided clues on the distribution and persistence of Arcobacter spp
Appl Environ Microbiol. 2024 Apr 22:e0029624. doi: 10.1128/aem.00296-24. Online ahead of print.ABSTRACTThe consumption of contaminated poultry meat is a significant threat for public health, as it implicates in foodborne pathogen infections, such as those caused by Arcobacter. The mitigation of clinical cases requires the understanding of contamination pathways in each food process and the characterization of resident microbiota in the productive environments, so that targeted sanitizing procedures can be effectively implemented. Nowadays these investigations can benefit from the complementary and thoughtful use of culture...
Source: Applied and Environmental Microbiology - April 22, 2024 Category: Microbiology Authors: Cristian Botta Davide Buzzanca Elisabetta Chiarini Francesco Chiesa Selene Rubiola Ilario Ferrocino Edoardo Fontanella Kalliopi Rantsiou Kurt Houf Valentina Alessandria Source Type: research

Articles of Significant Interest in This Issue
Appl Environ Microbiol. 2024 Apr 17;90(4):e0066424. doi: 10.1128/aem.00664-24. Epub 2024 Apr 17.NO ABSTRACTPMID:38629879 | PMC:PMC11022557 | DOI:10.1128/aem.00664-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - April 17, 2024 Category: Microbiology Source Type: research

Articles of Significant Interest in This Issue
Appl Environ Microbiol. 2024 Apr 17;90(4):e0066424. doi: 10.1128/aem.00664-24. Epub 2024 Apr 17.NO ABSTRACTPMID:38629879 | DOI:10.1128/aem.00664-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - April 17, 2024 Category: Microbiology Source Type: research

Warming alters life-history traits and competition in a phage community
In this study, we investigate the impact of warming across a fever temperature range (37°C, 40°C, and 42°C) on phage life-history traits and competition using a bacterium (host) and bacteriophage (parasite) system. We show that phages vary in their responses to temperature due to disruption of different phage life-history traits. Furthermore, we show that temperature can alter phage competitiveness and shape phage-phage competition outcomes. These results suggest that fever temperatures have the potential to restrict phage infectivity and drive phage community dynamics. We discuss implications for the role of temperatur...
Source: Applied and Environmental Microbiology - April 16, 2024 Category: Microbiology Authors: Samuel T E Greenrod Daniel Cazares Serena Johnson Tobias E Hector Emily J Stevens R Craig MacLean Kayla C King Source Type: research

Polysaccharide intercellular adhesin and proper phospholipid composition are important for aggregation in Tetragenococcus halophilus SL10
This study provides evidence for the presence of aggregation factors in T. halophilus other than the proteinaceous aggregation factor and suggests that further understanding of the coordinated action of these factors may improve clarified soy sauce production.PMID:38624197 | DOI:10.1128/aem.00334-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - April 16, 2024 Category: Microbiology Authors: Airi Yanagihara Kouta Matsue Kurumi Kobayashi Takura Wakinaka Yoshinobu Mogi Jun Watanabe Source Type: research

Old role with new feature: T2SS ATPase as a cyclic-di-GMP receptor to regulate antibiotic production
In this study, we identified LspE, a homologous protein of the type II secretion system (T2SS) ATPase GspE in Lysobacter enzymogenes, as a receptor protein for c-di-GMP. We identified the more conservative c-di-GMP binding amino acid residues as K358 and T359, which differ from the previous reports, indicating that GspE proteins may represent a class of c-di-GMP receptor proteins. Additionally, we found that LspE in L. enzymogenes also possesses a novel role in regulating the production of the antifungal antibiotic HSAF. Further investigations revealed the critical involvement of both ATPase activity and c-di-GMP binding i...
Source: Applied and Environmental Microbiology - April 16, 2024 Category: Microbiology Authors: Haofei Liu Gaoge Xu Baodian Guo Fengquan Liu Source Type: research

A Burkholderia cenocepacia -like environmental isolate strongly inhibits the plant fungal pathogen Zymoseptoria tritici 
Appl Environ Microbiol. 2024 Apr 16:e0222223. doi: 10.1128/aem.02222-23. Online ahead of print.ABSTRACTFungal phytopathogens cause significant reductions in agricultural yields annually, and overusing chemical fungicides for their control leads to environmental pollution and the emergence of resistant pathogens. Exploring natural isolates with strong antagonistic effects against pathogens can improve our understanding of their ecology and develop new treatments for the future. We isolated and characterized a novel bacterial strain associated with the species Burkholderia cenocepacia, termed APO9, which strongly inhibits Zy...
Source: Applied and Environmental Microbiology - April 16, 2024 Category: Microbiology Authors: Tingting Song Suyash Gupta Yael Sorokin Omer Frenkel Eddie Cytryn Jonathan Friedman Source Type: research

Identification and enzymatic properties of arginine decarboxylase from Aspergillus oryzae 
Appl Environ Microbiol. 2024 Apr 16:e0029424. doi: 10.1128/aem.00294-24. Online ahead of print.ABSTRACTAspergillus oryzae spores, when sprinkled onto steamed rice and allowed to propagate, are referred to as rice "koji." Agmatine, a natural polyamine derived from arginine through the action of arginine decarboxylase (ADC), is abundantly produced by solid state-cultivated rice koji of A. oryzae RIB40 under low pH conditions, despite the apparent absence of ADC orthologs in its genome. Mass spectrometry imaging revealed that agmatine was accumulated inside rice koji at low pH conditions, where arginine was distributed. ADC a...
Source: Applied and Environmental Microbiology - April 16, 2024 Category: Microbiology Authors: Yui Murakami Soichiro Ikuta Wakao Fukuda Naoki Akasaka Jun-Ichi Maruyama Shuichi Shinma Eiichiro Fukusaki Shinsuke Fujiwara Source Type: research