Applied and Environmental Microbiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Antibacterial insights into alternariol and its derivative alternariol monomethyl ether produced by a marine fungus
Appl Environ Microbiol. 2024 Mar 12:e0005824. doi: 10.1128/aem.00058-24. Online ahead of print.ABSTRACTAlternaria alternata FB1 is a marine fungus identified as a candidate for plastic degradation in our previous study. This fungus has been recently shown to produce secondary metabolites with significant antimicrobial activity against various pathogens, including methicillin-resistant Staphylococcus aureus (MRSA) and the notorious aquaculture pathogen Vibrio anguillarum. The antibacterial compounds were purified and identified as alternariol (AOH) and its derivative, alternariol monomethyl ether (AME). We found that AOH an...
Source: Applied and Environmental Microbiology - March 12, 2024 Category: Microbiology Authors: Rongmei Li Zhenjie Su Chaomin Sun Shimei Wu Source Type: research

Pyoluteorin regulates the biosynthesis of 2,4-DAPG through the TetR family transcription factor PhlH in Pseudomonas protegens Pf-5
In this study, we find that the TetR family transcription factor PhlH is involved in the cross talk between pyoluteorin and 2,4-DAPG biosynthesis. PhlH binds to a palindromic sequence within the promoter of phlG (PphlG), which encodes a C-C bond hydrolase responsible for degrading 2,4-DAPG. As a signaling molecule, pyoluteorin disrupts the PhlH-PphlG complex by binding to PhlH, leading to decreased levels of 2,4-DAPG. Proteomics data suggest that pyoluteorin regulates multiple physiological processes including fatty acid biosynthesis and transportation of taurine, siderophore, and amino acids. Our work not only reveals a n...
Source: Applied and Environmental Microbiology - March 12, 2024 Category: Microbiology Authors: Li-Ming Luo Hang Xu Nannan Zhang Honghua Ge Yun Xiang Hao Yang Yong-Xing He Source Type: research

Mitigation of Vibrio coralliilyticus -induced coral bleaching through bacterial dysbiosis prevention by Ruegeria profundi 
This study investigates how coral-associated bacterial communities respond to bleaching induced by V. coralliilyticus. Our findings demonstrate that Ruegeria profundi exhibits clear evidence of defense against pathogenic bacterial infection, contributing to the maintenance of host health and symbiont homeostasis. This observation suggests that bacterial pathogens could cause dysbiosis in coral holobionts. Probiotic bacteria display an essential capability in restructuring and manipulating coral-associated bacterial communities. This restructuring effectively reduces bacterial community virulence and enhances the pathogenic...
Source: Applied and Environmental Microbiology - March 12, 2024 Category: Microbiology Authors: Meiting Xu Zhonghua Cai Keke Cheng Guofu Chen Jin Zhou Source Type: research

Development of a genetic system for Haloferax gibbonsii LR2-5, model host for haloarchaeal viruses
Appl Environ Microbiol. 2024 Mar 12:e0012924. doi: 10.1128/aem.00129-24. Online ahead of print.ABSTRACTArchaeal viruses are among the most enigmatic members of the virosphere, and their diverse morphologies raise many questions about their infection mechanisms. The study of molecular mechanisms underlying virus-host interactions hinges upon robust model organisms with a system for gene expression and deletion. Currently, there are only a limited number of archaea that have associated viruses and have a well-developed genetic system. Here, we report the development of a genetic system for the euryarchaeon Haloferax gibbonsi...
Source: Applied and Environmental Microbiology - March 12, 2024 Category: Microbiology Authors: Colin Tittes Jeroen Nijland Anna M C Schoentag Thomas Hackl Nadia Di Cianni Anita Marchfelder Tessa E F Quax Source Type: research

Microbiome analysis of the restricted bacteria in radioactive element-containing water at the Fukushima Daiichi Nuclear Power Station
This study represents the first comprehensive analysis of microbial communities, utilizing meta 16S amplicon sequencing, with a focus on environmental samples collected from the radioactive element-containing water in the torus room, including the surrounding environments. Some of the identified microbial genera are shared with those previously identified in spent nuclear fuel pools in countries such as France and Brazil. Moreover, our discussion in this paper elucidates the correlation of many of these bacteria with metal corrosion.PMID:38470121 | DOI:10.1128/aem.02113-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - March 12, 2024 Category: Microbiology Authors: Tomoro Warashina Asako Sato Hiroshi Hinai Nurislam Shaikhutdinov Elena Shagimardanova Hiroshi Mori Satoshi Tamaki Motofumi Saito Yukihisa Sanada Yoshito Sasaki Kozue Shimada Yuma Dotsuta Toru Kitagaki Shigenori Maruyama Oleg Gusev Issay Narumi Ken Kurokaw Source Type: research

Comparison of the performance of multiple whole-genome sequence-based tools for the identification of Bacillus cereus sensu stricto biovar Thuringiensis 
This study assessed the accuracy of whole-genome sequencing (WGS)-based identification of Bt compared to phenotypic microscopy-based screening for crystal protein production. Multiple bioinformatics tools were compared to assess their performance in predicting crystal protein production. Among them, identification of pesticidal sequences performed best overall at WGS-based Bt identification.PMID:38470126 | DOI:10.1128/aem.01778-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - March 12, 2024 Category: Microbiology Authors: Taejung Chung Abimel Salazar Grant Harm Sophia Johler Laura M Carroll Jasna Kovac Source Type: research

Queen bee gut microbiota extends honeybee lifespan by inhibiting insulin signaling
Appl Environ Microbiol. 2024 Mar 12:e0179923. doi: 10.1128/aem.01799-23. Online ahead of print.ABSTRACTQueen and worker bees are natural models for aging research, as their lifespans vary considerably independent of genetic variation. Investigating the reasons why queens live longer than workers is of great significance for research on the universal processes of aging in animals. The gut microbiome has received attention as a vital regulator of host health, while its precise role in honeybee aging needs further investigation. The effects and mechanisms behind the relationship between gut microbiota and worker lifespan were...
Source: Applied and Environmental Microbiology - March 12, 2024 Category: Microbiology Authors: Hongfang Wang Wenfeng Chen Li Lei Wei Zhang Zhenguo Liu Ying Wang Baohua Xu Source Type: research

The prokaryotic and eukaryotic microbiome of Pacific oyster spat is shaped by ocean warming but not acidification
Appl Environ Microbiol. 2024 Mar 11:e0005224. doi: 10.1128/aem.00052-24. Online ahead of print.ABSTRACTPacific oysters (Magallana gigas, a.k.a. Crassostrea gigas), the most widely farmed oysters, are under threat from climate change and emerging pathogens. In part, their resilience may be affected by their microbiome, which, in turn, may be influenced by ocean warming and acidification. To understand these impacts, we exposed early-development Pacific oyster spat to different temperatures (18°C and 24°C) and pCO2 levels (800, 1,600, and 2,800 µatm) in a fully crossed design for 3 weeks. Under all conditions, the microbi...
Source: Applied and Environmental Microbiology - March 11, 2024 Category: Microbiology Authors: Kevin Xu Zhong Amy M Chan Brenna Collicutt Maxim Daspe Jan F Finke Megan Foss Timothy J Green Christopher D G Harley Amelia V Hesketh Kristina M Miller Sarah P Otto Kate Rolheiser Rob Saunders Ben J G Sutherland Curtis A Suttle Source Type: research

Functional analysis of feedback inhibition-insensitive aspartate kinase identified in a threonine-accumulating mutant of Saccharomyces cerevisiae 
In this study, we isolated a mutant (strain HNV-5) that accumulates threonine, an EAA, derived from a diploid laboratory yeast by conventional mutagenesis. Strain HNV-5 carries a novel mutation in the HOM3 gene encoding the Ala462Thr variant of aspartate kinase (AK). Enzymatic analysis revealed that the Ala462Thr substitution significantly decreased the sensitivity of AK activity to threonine feedback inhibition even in the presence of 50 mM threonine. Interestingly, Ala462Thr substitution did not affect the catalytic ability of Hom3, in contrast to previously reported amino acid substitutions that resulted in reduced sens...
Source: Applied and Environmental Microbiology - March 8, 2024 Category: Microbiology Authors: Shota Isogai Akira Nishimura Akiko Inoue Shino Sonohara Takashi Tsugukuni Tomoyuki Okada Hiroshi Takagi Source Type: research

Plasmalogen, a glycerophospholipid crucial for Streptococcus mutans acid tolerance and colonization
This study sheds light on the biological role of plasmalogen, a specific glycerophospholipid, in bacteria, particularly in Streptococcus mutans. Plasmalogens are known for their significant roles in eukaryotic cells and have been linked to human diseases like neurological disorders and cancers. The enzyme SMU_438c, identified as essential for plasmalogen production under anaerobic conditions, was crucial for acid tolerance and in vivo colonization in Drosophila by S. mutans, underscoring its importance in bacterial stress response and colonization. These findings bridge the knowledge gap in bacterial physiology, highlighti...
Source: Applied and Environmental Microbiology - March 8, 2024 Category: Microbiology Authors: Rong Mu Stephanie Momeni Madeline Krieger Baotong Xie Xixi Cao Justin Merritt Hui Wu Source Type: research