Retraction for Harrison et al., "Molecular Characterization of < em > Clostridium perfringens < /em > Isolates from Humans with Sporadic Diarrhea: Evidence for Transcriptional Regulation of the Beta2-Toxin-Encoding Gene"
Appl Environ Microbiol. 2024 Apr 12:e0025924. doi: 10.1128/aem.00259-24. Online ahead of print.NO ABSTRACTPMID:38606962 | DOI:10.1128/aem.00259-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - April 12, 2024 Category: Microbiology Authors: Ben Harrison Deepa Raju Helen S Garmory Moira M Brett Richard W Titball Mahfuzur R Sarker Source Type: research

Design of a tunable bacterial gene expression system using engineered σ factors
Appl Environ Microbiol. 2024 Apr 12:e0002124. doi: 10.1128/aem.00021-24. Online ahead of print.ABSTRACTExtracytoplasmic function (ECF) σ factors selectively upregulate expression of specific genes in bacteria. These σ factors, belonging to the σ70 family, are much smaller than the primary, housekeeping σ factor with two helical domains that interact with the Pribnow box and the -35 element of the promoter DNA. Structural studies reveal that promoter specificity in a σ factor is determined by the interactions between a loop (L3) and the Pribnow box element. Similarly, the efficiency of transcription initiation is gover...
Source: Applied and Environmental Microbiology - April 12, 2024 Category: Microbiology Authors: Twinkal Patel Amit Dinda Sankar Mahesh Savitha Nadig Nishank Reddy Balasubramanian Gopal Source Type: research

Retraction for Sarker et al., "Comparative Experiments To Examine the Effects of Heating on Vegetative Cells and Spores of < em > Clostridium perfringens < /em > Isolates Carrying Plasmid Genes versus Chromosomal Enterotoxin Genes"
Appl Environ Microbiol. 2024 Apr 12:e0024924. doi: 10.1128/aem.00249-24. Online ahead of print.NO ABSTRACTPMID:38606961 | DOI:10.1128/aem.00249-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - April 12, 2024 Category: Microbiology Authors: Mahfuzur R Sarker Robert P Shivers Shauna G Sparks Vijay K Juneja Bruce A McClane Source Type: research

Retraction for Harrison et al., "Molecular Characterization of < em > Clostridium perfringens < /em > Isolates from Humans with Sporadic Diarrhea: Evidence for Transcriptional Regulation of the Beta2-Toxin-Encoding Gene"
Appl Environ Microbiol. 2024 Apr 12:e0025924. doi: 10.1128/aem.00259-24. Online ahead of print.NO ABSTRACTPMID:38606962 | DOI:10.1128/aem.00259-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - April 12, 2024 Category: Microbiology Authors: Ben Harrison Deepa Raju Helen S Garmory Moira M Brett Richard W Titball Mahfuzur R Sarker Source Type: research

Design of a tunable bacterial gene expression system using engineered σ factors
Appl Environ Microbiol. 2024 Apr 12:e0002124. doi: 10.1128/aem.00021-24. Online ahead of print.ABSTRACTExtracytoplasmic function (ECF) σ factors selectively upregulate expression of specific genes in bacteria. These σ factors, belonging to the σ70 family, are much smaller than the primary, housekeeping σ factor with two helical domains that interact with the Pribnow box and the -35 element of the promoter DNA. Structural studies reveal that promoter specificity in a σ factor is determined by the interactions between a loop (L3) and the Pribnow box element. Similarly, the efficiency of transcription initiation is gover...
Source: Applied and Environmental Microbiology - April 12, 2024 Category: Microbiology Authors: Twinkal Patel Amit Dinda Sankar Mahesh Savitha Nadig Nishank Reddy Balasubramanian Gopal Source Type: research

Retraction for Sarker et al., "Comparative Experiments To Examine the Effects of Heating on Vegetative Cells and Spores of < em > Clostridium perfringens < /em > Isolates Carrying Plasmid Genes versus Chromosomal Enterotoxin Genes"
Appl Environ Microbiol. 2024 Apr 12:e0024924. doi: 10.1128/aem.00249-24. Online ahead of print.NO ABSTRACTPMID:38606961 | DOI:10.1128/aem.00249-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - April 12, 2024 Category: Microbiology Authors: Mahfuzur R Sarker Robert P Shivers Shauna G Sparks Vijay K Juneja Bruce A McClane Source Type: research

Retraction for Harrison et al., "Molecular Characterization of < em > Clostridium perfringens < /em > Isolates from Humans with Sporadic Diarrhea: Evidence for Transcriptional Regulation of the Beta2-Toxin-Encoding Gene"
Appl Environ Microbiol. 2024 Apr 12:e0025924. doi: 10.1128/aem.00259-24. Online ahead of print.NO ABSTRACTPMID:38606962 | DOI:10.1128/aem.00259-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - April 12, 2024 Category: Microbiology Authors: Ben Harrison Deepa Raju Helen S Garmory Moira M Brett Richard W Titball Mahfuzur R Sarker Source Type: research

Design of a tunable bacterial gene expression system using engineered σ factors
Appl Environ Microbiol. 2024 Apr 12:e0002124. doi: 10.1128/aem.00021-24. Online ahead of print.ABSTRACTExtracytoplasmic function (ECF) σ factors selectively upregulate expression of specific genes in bacteria. These σ factors, belonging to the σ70 family, are much smaller than the primary, housekeeping σ factor with two helical domains that interact with the Pribnow box and the -35 element of the promoter DNA. Structural studies reveal that promoter specificity in a σ factor is determined by the interactions between a loop (L3) and the Pribnow box element. Similarly, the efficiency of transcription initiation is gover...
Source: Applied and Environmental Microbiology - April 12, 2024 Category: Microbiology Authors: Twinkal Patel Amit Dinda Sankar Mahesh Savitha Nadig Nishank Reddy Balasubramanian Gopal Source Type: research

Redefining piscine lactococcosis
Appl Environ Microbiol. 2024 Apr 10:e0234923. doi: 10.1128/aem.02349-23. Online ahead of print.ABSTRACTPiscine lactococcosis is a significant threat to cultured and wild fish populations worldwide. The disease typically presents as a per-acute to acute hemorrhagic septicemia causing high morbidity and mortality, recalcitrant to antimicrobial treatment or management interventions. Historically, the disease was attributed to the gram-positive pathogen Lactococcus garvieae. However, recent work has revealed three distinct lactococcosis-causing bacteria (LCB)-L. garvieae, L. petauri, and L. formosensis-which are phenotypically...
Source: Applied and Environmental Microbiology - April 10, 2024 Category: Microbiology Authors: Taylor I Heckman Zeinab Yazdi Caitlin E Older Matt J Griffin Geoffrey C Waldbieser Alexander M Chow Isabella Medina Silva Kelsey M Anenson Julio C Garc ía Benjamin R LaFrentz Durda Slavic Kathy L Toohey-Kurth Paula Yant Heather M Fritz Eileen E Henderson Source Type: research

An autonomous plasmid as an inovirus phage satellite
Appl Environ Microbiol. 2024 Apr 10:e0024624. doi: 10.1128/aem.00246-24. Online ahead of print.ABSTRACTBacterial viruses (phages) are potent agents of lateral gene transfer and thus are important drivers of evolution. A group of mobile genetic elements, referred to as phage satellites, exploits phages to disseminate their own genetic material. Here, we isolated a novel member of the family Inoviridae, Shewanella phage Dolos, along with an autonomously replicating plasmid, pDolos. Dolos causes a chronic infection in its host Shewanella oneidensis by phage production with only minor effects on the host cell proliferation. Wh...
Source: Applied and Environmental Microbiology - April 10, 2024 Category: Microbiology Authors: Nicole Schmid David Brandt Claudia Walasek Clara Rolland Johannes Wittmann Dorian Fischer Mathias M üsken J örn Kalinowski Kai Thormann Source Type: research

Redefining piscine lactococcosis
Appl Environ Microbiol. 2024 Apr 10:e0234923. doi: 10.1128/aem.02349-23. Online ahead of print.ABSTRACTPiscine lactococcosis is a significant threat to cultured and wild fish populations worldwide. The disease typically presents as a per-acute to acute hemorrhagic septicemia causing high morbidity and mortality, recalcitrant to antimicrobial treatment or management interventions. Historically, the disease was attributed to the gram-positive pathogen Lactococcus garvieae. However, recent work has revealed three distinct lactococcosis-causing bacteria (LCB)-L. garvieae, L. petauri, and L. formosensis-which are phenotypically...
Source: Applied and Environmental Microbiology - April 10, 2024 Category: Microbiology Authors: Taylor I Heckman Zeinab Yazdi Caitlin E Older Matt J Griffin Geoffrey C Waldbieser Alexander M Chow Isabella Medina Silva Kelsey M Anenson Julio C Garc ía Benjamin R LaFrentz Durda Slavic Kathy L Toohey-Kurth Paula Yant Heather M Fritz Eileen E Henderson Source Type: research

An autonomous plasmid as an inovirus phage satellite
Appl Environ Microbiol. 2024 Apr 10:e0024624. doi: 10.1128/aem.00246-24. Online ahead of print.ABSTRACTBacterial viruses (phages) are potent agents of lateral gene transfer and thus are important drivers of evolution. A group of mobile genetic elements, referred to as phage satellites, exploits phages to disseminate their own genetic material. Here, we isolated a novel member of the family Inoviridae, Shewanella phage Dolos, along with an autonomously replicating plasmid, pDolos. Dolos causes a chronic infection in its host Shewanella oneidensis by phage production with only minor effects on the host cell proliferation. Wh...
Source: Applied and Environmental Microbiology - April 10, 2024 Category: Microbiology Authors: Nicole Schmid David Brandt Claudia Walasek Clara Rolland Johannes Wittmann Dorian Fischer Mathias M üsken J örn Kalinowski Kai Thormann Source Type: research

Redefining piscine lactococcosis
Appl Environ Microbiol. 2024 Apr 10:e0234923. doi: 10.1128/aem.02349-23. Online ahead of print.ABSTRACTPiscine lactococcosis is a significant threat to cultured and wild fish populations worldwide. The disease typically presents as a per-acute to acute hemorrhagic septicemia causing high morbidity and mortality, recalcitrant to antimicrobial treatment or management interventions. Historically, the disease was attributed to the gram-positive pathogen Lactococcus garvieae. However, recent work has revealed three distinct lactococcosis-causing bacteria (LCB)-L. garvieae, L. petauri, and L. formosensis-which are phenotypically...
Source: Applied and Environmental Microbiology - April 10, 2024 Category: Microbiology Authors: Taylor I Heckman Zeinab Yazdi Caitlin E Older Matt J Griffin Geoffrey C Waldbieser Alexander M Chow Isabella Medina Silva Kelsey M Anenson Julio C Garc ía Benjamin R LaFrentz Durda Slavic Kathy L Toohey-Kurth Paula Yant Heather M Fritz Eileen E Henderson Source Type: research

An autonomous plasmid as an inovirus phage satellite
Appl Environ Microbiol. 2024 Apr 10:e0024624. doi: 10.1128/aem.00246-24. Online ahead of print.ABSTRACTBacterial viruses (phages) are potent agents of lateral gene transfer and thus are important drivers of evolution. A group of mobile genetic elements, referred to as phage satellites, exploits phages to disseminate their own genetic material. Here, we isolated a novel member of the family Inoviridae, Shewanella phage Dolos, along with an autonomously replicating plasmid, pDolos. Dolos causes a chronic infection in its host Shewanella oneidensis by phage production with only minor effects on the host cell proliferation. Wh...
Source: Applied and Environmental Microbiology - April 10, 2024 Category: Microbiology Authors: Nicole Schmid David Brandt Claudia Walasek Clara Rolland Johannes Wittmann Dorian Fischer Mathias M üsken J örn Kalinowski Kai Thormann Source Type: research

< em > Saccharomyces cerevisiae < /em > strains performing similarly during fermentation of lignocellulosic hydrolysates show pronounced differences in transcriptional stress responses
Appl Environ Microbiol. 2024 Apr 8:e0233023. doi: 10.1128/aem.02330-23. Online ahead of print.ABSTRACTImproving our understanding of the transcriptional changes of Saccharomyces cerevisiae during fermentation of lignocellulosic hydrolysates is crucial for the creation of more efficient strains to be used in biorefineries. We performed RNA sequencing of a CEN.PK laboratory strain, two industrial strains (KE6-12 and Ethanol Red), and two wild-type isolates of the LBCM collection when cultivated anaerobically in wheat straw hydrolysate. Many of the differently expressed genes identified among the strains have previously been ...
Source: Applied and Environmental Microbiology - April 8, 2024 Category: Microbiology Authors: Elena C ámara Maurizio Mormino Verena Siewers Yvonne Nyg ård Source Type: research