Applied and Environmental Microbiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Exploring functional genes' correlation with ( S )-equol concentration and new daidzein racemase identification
This study examines the association between functional genes and (S)-EQ production, highlighting a potential correlation between the DZNR gene and (S)-EQ content. Various types of DZNR may be linked to the regulation of (S)-EQ synthesis. Furthermore, the identification of a new DDRC gene offers promising prospects for enhancing in vitro (S)-EQ production.PMID:38501861 | DOI:10.1128/aem.00007-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - March 19, 2024 Category: Microbiology Authors: Yun-Fei Hu Shu Luo Sheng-Qi Wang Ke-Xin Chen Wei-Xuan Zhong Bai-Yuan Li Lin-Yan Cao Hua-Hai Chen Ye-Shi Yin Source Type: research

Protein disulfide isomerase 1 is required for RodA assembling-based conidial hydrophobicity of Aspergillus fumigatus 
In conclusion, Pdi1 ensures the correct folding of RodA in the inner layer of conidia, facilitating its secretion into the outer layer of the cell wall and allowing self-assembly of the hydrophobic layer. This study has identified a regulatory element for conidia rodlet assembly.IMPORTANCEAspergillus fumigatus is the major cause of invasive aspergillosis, which is mainly transmitted by the inhalation of conidia. The spread of conidia is largely dependent on their hydrophobicity, which is primarily attributed to the self-assembly of the hydrophobic protein RodA on the cell wall. However, the mechanisms underlying RodA secre...
Source: Applied and Environmental Microbiology - March 19, 2024 Category: Microbiology Authors: Xiaotao Hu Yao Zhou Renning Liu Jing Wang Lingyan Guo Xiaofei Huang Jingyi Li Yunfeng Yan Feiyun Liu Xueying Li Xinyu Tan Yiqing Luo Ping Wang Shengmin Zhou Source Type: research

Combining CRISPR/Cas9 and natural excision for the precise and complete removal of mobile genetic elements in bacteria
Appl Environ Microbiol. 2024 Mar 18:e0009524. doi: 10.1128/aem.00095-24. Online ahead of print.ABSTRACTHorizontal gene transfer, facilitated by mobile genetic elements (MGEs), is an adaptive evolutionary process that contributes to the evolution of bacterial populations and infectious diseases. A variety of MGEs not only can integrate into the bacterial genome but also can survive or even replicate like plasmids in the cytoplasm, thus requiring precise and complete removal for studying their strategies in benefiting host cells. Existing methods for MGE removal, such as homologous recombination-based deletion and excisionas...
Source: Applied and Environmental Microbiology - March 18, 2024 Category: Microbiology Authors: Pengxia Wang Xiaofei Du Yi Zhao Weiquan Wang Tongxuan Cai Kaihao Tang Xiaoxue Wang Source Type: research

Exploring associations between the teat apex metagenome and Staphylococcus aureus intramammary infections in primiparous cows under organic directives
Appl Environ Microbiol. 2024 Mar 18:e0223423. doi: 10.1128/aem.02234-23. Online ahead of print.ABSTRACTThe primary objective of this study was to identify associations between the prepartum teat apex microbiome and the presence of Staphylococcus aureus intramammary infections (IMI) in primiparous cows during the first 5 weeks after calving. We performed a case-control study using shotgun metagenomics of the teat apex and culture-based milk data collected longitudinally from 710 primiparous cows on five organic dairy farms. Cases had higher odds of having S. aureus metagenomic DNA on the teat apex prior to parturition compa...
Source: Applied and Environmental Microbiology - March 18, 2024 Category: Microbiology Authors: C J Dean F Pe ña-Mosca T Ray T J Wehri K Sharpe A M Antunes E Doster L Fernandes V F Calles C Bauman S Godden B Heins P Pinedo V S Machado L S Caixeta N R Noyes Source Type: research

Eye infection risks from Pseudomonas aeruginosa via hand soap and eye drops
This study applies the risk assessment framework of QMRA to evaluate eye infection risks through both consumer products. The study examines the prevalence of this pathogen in eye drops and soap, as well as the critical need to implement measures that will mitigate bacterial exposure (e.g., single-use soap dispensers and eye drops with preservatives). Additionally, limitations and challenges are discussed, including the need to incorporate data regarding consumer practices, which may improve exposure assessments and health risk estimates.PMID:38497644 | DOI:10.1128/aem.02119-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - March 18, 2024 Category: Microbiology Authors: Anna Gitter Kristina D Mena Karla S Mendez Fuqing Wu Charles P Gerba Source Type: research

A novel glycoside hydrolase 43-like enzyme from Clostridium boliviensis is an endo-xylanase and a candidate for xylooligosaccharide production from different xylan substrates
In conclusion, CbE1Xyn43-l from C. boliviensis strain E-1 is the first characterized member of a large group of homologous hypothetical proteins annotated as GH43-like and is a thermostable endo-xylanase, producing xylooligosaccharides of high DP (xylotriose and xylotetraose) producer.IMPORTANCE: The genome of Clostridium boliviensis strain E-1 encodes a number of hypothetical enzymes, annotated as glycoside hydrolase-like but not classified in the Carbohydrate Active Enzyme Database (CAZy). A novel thermostable GH43-like enzyme is here characterized as an endo-β-xylanase of interest in the production of prebiotic xylooli...
Source: Applied and Environmental Microbiology - March 18, 2024 Category: Microbiology Authors: Daniel Martin Salas-Veizaga Leonardo Roberto Rocabado-Villegas Javier A Linares-Past én Elisabet Eik Gudmundsdottir Gudmundur Oli Hreggvidsson Mar ía Teresa Álvarez-Aliaga Patrick Adlercreutz Eva Nordberg Karlsson Source Type: research

Impacts of litter microbial community on litter decomposition in the absence of soil microorganisms
This study has enriched our understanding of the decomposition of litter itself and provided a theoretical basis for further exploring the "additive and non-additive effects" of litter decomposition and the mechanism of microbial drive.IMPORTANCE: The study of litter decomposition mechanism plays an important role in the material circulation of the global ecosystem. However, previous studies have often looked at contact with soil as the starting point for decomposition. But actually, standing litter is very common in forest ecosystems. Therefore, we used field simulation experiments to simulate the decomposition of litters...
Source: Applied and Environmental Microbiology - March 14, 2024 Category: Microbiology Authors: Jiaying Liu Changjun Ding Chao Teng Weixi Zhang Xiaohua Su Wenxu Zhu Source Type: research

The white rot basidiomycete Gelatoporia subvermispora produces fatty aldehydes that enable fungal manganese peroxidases to degrade recalcitrant lignin structures
Appl Environ Microbiol. 2024 Mar 14:e0204423. doi: 10.1128/aem.02044-23. Online ahead of print.ABSTRACTThe ability of some white rot basidiomycetes to remove lignin selectively from wood indicates that low molecular weight oxidants have a role in ligninolysis. These oxidants are likely free radicals generated by fungal peroxidases from compounds in the biodegrading wood. Past work supports a role for manganese peroxidases (MnPs) in the production of ligninolytic oxidants from fungal membrane lipids. However, the fatty acid alkylperoxyl radicals initially formed during this process are not reactive enough to attack the majo...
Source: Applied and Environmental Microbiology - March 14, 2024 Category: Microbiology Authors: Alexander N Kapich Hideki Suzuki Kolby C Hirth Elena Fern ández-Fueyo Angel T Mart ínez Carl J Houtman Kenneth E Hammel Source Type: research