Applied and Environmental Microbiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Pan-azole- and multi-fungicide-resistant Aspergillus fumigatus is widespread in the United States
In this study, we isolated azole-resistant A. fumigatus from multiple states and compared it to isolates from around the world. We show that A. fumigatus which is resistant to azoles and to other strictly agricultural fungicides is widespread in the U.S.PMID:38557086 | DOI:10.1128/aem.01782-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - April 1, 2024 Category: Microbiology Authors: B N Celia-Sanchez B Mangum L F G ómez Londoño C Wang B Shuman M T Brewer M Momany Source Type: research

A gold speciation that adds a second layer to synergistic gold-copper toxicity in Cupriavidus metallidurans 
This study reveals that the geochemical conditions may decide whether bacteria are able to form gold nanoparticles or not.PMID:38557120 | DOI:10.1128/aem.00146-24 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - April 1, 2024 Category: Microbiology Authors: Niklas Hirth Nicole Wiesemann Stephanie Kr üger Michelle-Sophie Gerlach Kilian Preu ßner Diana Galea Martin Herzberg Cornelia Gro ße Dietrich H Nies Source Type: research

CslA and GlxA from Streptomyces lividans form a functional cellulose synthase complex
Appl Environ Microbiol. 2024 Apr 1:e0208723. doi: 10.1128/aem.02087-23. Online ahead of print.ABSTRACTFilamentous growth of streptomycetes coincides with the synthesis and deposition of an uncharacterized protective glucan at hyphal tips. Synthesis of this glucan depends on the integral membrane protein CslA and the radical copper oxidase GlxA, which are part of a presumably large multiprotein complex operating at growing tips. Here, we show that CslA and GlxA interact by forming a protein complex that is sufficient to synthesize cellulose in vitro. Mass spectrometry analysis revealed that the purified complex produces cel...
Source: Applied and Environmental Microbiology - April 1, 2024 Category: Microbiology Authors: Xiaobo Zhong Simone Nicolardi Ruochen Ouyang Manfred Wuhrer Chao Du Gilles van Wezel Erik Vijgenboom Ariane Briegel Dennis Claessen Source Type: research

An NADH/NAD + -favored aldo-keto reductase facilitates avilamycin A biosynthesis by primarily catalyzing oxidation of avilamycin C
This study represents, to our knowledge, the first characterization of biochemical reactions involved in avilamycin biosynthesis and contributes to the construction of high-performance strains with industrial value.IMPORTANCEAvilamycins are a group of oligosaccharide antibiotics produced by Streptomyces viridochromogenes, which can be used as veterinary drugs and animal feed additives. Avilamycin A is the most bioactive component, differing from avilamycin C only in the redox state of the two-carbon branched-chain of the terminal octose moiety. Currently, the biosynthetic pathway of avilamycins is not clear. Here, we repor...
Source: Applied and Environmental Microbiology - March 29, 2024 Category: Microbiology Authors: Derundong Zhang Yaotong Wang Qunyan Tang Qinglin Zhang Xiaowei Ji Xiangqi Qiu Dandan Chen Wen Liu Source Type: research

Long-duration environmental biosensing by recording analyte detection in DNA using recombinase memory
Appl Environ Microbiol. 2024 Mar 29:e0236323. doi: 10.1128/aem.02363-23. Online ahead of print.ABSTRACTMicrobial biosensors that convert environmental information into real-time visual outputs are limited in their sensing abilities in complex environments, such as soil and wastewater, due to optical inaccessibility. Biosensors that could record transient exposure to analytes within a large time window for later retrieval represent a promising approach to solve the accessibility problem. Here, we test the performance of recombinase-memory biosensors that sense a sugar (arabinose) and a microbial communication molecule (3-ox...
Source: Applied and Environmental Microbiology - March 29, 2024 Category: Microbiology Authors: Prashant Bharadwaj Kalvapalle Swetha Sridhar Jonathan J Silberg Lauren B Stadler Source Type: research

A novel phage putative depolymerase, Depo16, has specific activity against K1 capsular-type Klebsiella pneumoniae 
In this study, the lytic bacteriophage vB_KpnP_ZK1 (abbreviated as ZK1) was isolated using serotype K1 hvKP as the host. Although amino acid sequence BLAST analysis indicated that the tail fiber protein Depo16 of phage ZK1 showed no significant similarity to any reported phage depolymerases, it displayed enzymatic activities that are characteristic of phage depolymerases. After expression and purification, Depo16 could efficiently remove the capsular polysaccharide layer that surrounds the surface of serotype K1 K. pneumoniae. Although no bactericidal activity was detected, Depo16 makes serotype K1 K. pneumoniae sensitive ...
Source: Applied and Environmental Microbiology - March 29, 2024 Category: Microbiology Authors: Rihong Zhao Shanshan Jiang Siyu Ren Li Yang Wenyu Han Zhimin Guo Jingmin Gu Source Type: research

Growth and mortality of aerobic anoxygenic phototrophs in the North Pacific Subtropical Gyre
Appl Environ Microbiol. 2024 Mar 29:e0003224. doi: 10.1128/aem.00032-24. Online ahead of print.ABSTRACTAerobic anoxygenic phototrophic (AAP) bacteria harvest light energy using bacteriochlorophyll-containing reaction centers to supplement their mostly heterotrophic metabolism. While their abundance and growth have been intensively studied in coastal environments, much less is known about their activity in oligotrophic open ocean regions. Therefore, we combined in situ sampling in the North Pacific Subtropical Gyre, north of O'ahu island, Hawaii, with two manipulation experiments. Infra-red epifluorescence microscopy docume...
Source: Applied and Environmental Microbiology - March 29, 2024 Category: Microbiology Authors: Michal Kobl ížek Isabel Ferrera Eva Kol ářová Solange Duhamel Kimberly J Popendorf Josep M Gasol Benjamin A S Van Mooy Source Type: research

Sulfate-reducing bacteria unearthed: ecological functions of the diverse prokaryotic group in terrestrial environments
Appl Environ Microbiol. 2024 Mar 29:e0139023. doi: 10.1128/aem.01390-23. Online ahead of print.ABSTRACTSulfate-reducing prokaryotes (SRPs) are essential microorganisms that play crucial roles in various ecological processes. Even though SRPs have been studied for over a century, there are still gaps in our understanding of their biology. In the past two decades, a significant amount of data on SRP ecology has been accumulated. This review aims to consolidate that information, focusing on SRPs in soils, their relation to the rare biosphere, uncultured sulfate reducers, and their interactions with other organisms in terrestr...
Source: Applied and Environmental Microbiology - March 29, 2024 Category: Microbiology Authors: Konstantin A Demin Evgeniya V Prazdnova Tatiana M Minkina Andrey V Gorovtsov Source Type: research