Evolution and separation of actinobacterial pyranose and < em > C < /em > -glycoside-3-oxidases
Appl Environ Microbiol. 2024 Jan 5:e0167623. doi: 10.1128/aem.01676-23. Online ahead of print.ABSTRACTC-Glycosides often form active compounds in various plants. Breakage of the C-C bond in these glycosides to release the aglycone is challenging and proceeds via a two-step reaction, the oxidation of the sugar and subsequent cleavage of the C-C bond. Recently, an enzyme from a soil bacterium, FAD-dependent C-glycoside-3-oxidase (CGOx), was shown to catalyze the initial oxidation reaction. Here, we show that CGOx belongs to the same sequence space as pyranose oxidase (POx), and that an actinobacterial ancestor of the POx/CGO...
Source: Applied and Environmental Microbiology - January 5, 2024 Category: Microbiology Authors: Anja Kostelac Andr é Taborda L ígia O Martins Dietmar Haltrich Source Type: research

AlmA involved in the long-chain < em > n < /em > -alkane degradation pathway in < em > Acinetobacter baylyi < /em > ADP1 is a Baeyer-Villiger monooxygenase
In this study, we conducted a detailed characterization of AlmA in A. baylyi ADP1 and found that AlmA exhibits Baeyer-Villiger monooxygenase activity, thus indicating the presence of a novel LC n-alkane biodegradation mechanism in strain ADP1.PMID:38168668 | DOI:10.1128/aem.01625-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - January 3, 2024 Category: Microbiology Authors: Chao-Fan Yin Yong Nie Tao Li Ning-Yi Zhou Source Type: research

The catabolic specialization of the marine bacterium < em > Polaribacter < /em > sp. Q13 to red algal β1,3/1,4-mixed-linkage xylan
This study reveals the catabolism of MLX by marine Bacteroidetes, promoting our understanding of the degradation and utilization of algal polysaccharides by marine bacteria. This study also sets a foundation for the biomass conversion of MLX.PMID:38169280 | DOI:10.1128/aem.01704-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - January 3, 2024 Category: Microbiology Authors: Fang Zhao Chun-Mei Yu Hai-Ning Sun Ting-Ting Xu Zhong-Zhi Sun Qi-Long Qin Ning Wang Xiu-Lan Chen Yang Yu Yu-Zhong Zhang Source Type: research

< em > Marinomonas mediterranea < /em > synthesizes an R-type bacteriocin
In this study, it is shown that some extracellular defective phage particles previously observed in Marinomonas mediterranea are in fact R-type bacteriocins showing antimicrobial activity against other Marinomonas strains. The operon coding for the R-type bacteriocin has been identified.PMID:38169292 | DOI:10.1128/aem.01273-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - January 3, 2024 Category: Microbiology Authors: Patricia Lucas-El ío Tarik ElAlami Alicia Mart ínez Antonio Sanchez-Amat Source Type: research

The military gear microbiome: risk factors surrounding the warfighter
Appl Environ Microbiol. 2024 Jan 3:e0117623. doi: 10.1128/aem.01176-23. Online ahead of print.ABSTRACTCombat extremity wounds are vulnerable to contamination from environments of proximity to the warfighter, leading to potential detrimental outcomes such as infection and delayed wound healing. Therefore, microbial surveillance of such environments is necessary to aid the advancement of military safety and preparedness through clinical diagnostics, treatment protocols, and uniform material design.PMID:38170999 | DOI:10.1128/aem.01176-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - January 3, 2024 Category: Microbiology Authors: Car Reen Kok Zakariae Bram James B Thissen Timothy S Horseman Keith S K Fong Susan A Reichert-Scrivner Carmen Paguirigan Kelsey O'Connor Kristina Thompson Alexander E Scheiber Shalini Mabery Viseth Ngauy Catherine F Uyehara Nicholas A Be Source Type: research

AlmA involved in the long-chain < em > n < /em > -alkane degradation pathway in < em > Acinetobacter baylyi < /em > ADP1 is a Baeyer-Villiger monooxygenase
In this study, we conducted a detailed characterization of AlmA in A. baylyi ADP1 and found that AlmA exhibits Baeyer-Villiger monooxygenase activity, thus indicating the presence of a novel LC n-alkane biodegradation mechanism in strain ADP1.PMID:38168668 | DOI:10.1128/aem.01625-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - January 3, 2024 Category: Microbiology Authors: Chao-Fan Yin Yong Nie Tao Li Ning-Yi Zhou Source Type: research

The catabolic specialization of the marine bacterium < em > Polaribacter < /em > sp. Q13 to red algal β1,3/1,4-mixed-linkage xylan
This study reveals the catabolism of MLX by marine Bacteroidetes, promoting our understanding of the degradation and utilization of algal polysaccharides by marine bacteria. This study also sets a foundation for the biomass conversion of MLX.PMID:38169280 | DOI:10.1128/aem.01704-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - January 3, 2024 Category: Microbiology Authors: Fang Zhao Chun-Mei Yu Hai-Ning Sun Ting-Ting Xu Zhong-Zhi Sun Qi-Long Qin Ning Wang Xiu-Lan Chen Yang Yu Yu-Zhong Zhang Source Type: research

< em > Marinomonas mediterranea < /em > synthesizes an R-type bacteriocin
In this study, it is shown that some extracellular defective phage particles previously observed in Marinomonas mediterranea are in fact R-type bacteriocins showing antimicrobial activity against other Marinomonas strains. The operon coding for the R-type bacteriocin has been identified.PMID:38169292 | DOI:10.1128/aem.01273-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - January 3, 2024 Category: Microbiology Authors: Patricia Lucas-El ío Tarik ElAlami Alicia Mart ínez Antonio Sanchez-Amat Source Type: research

The military gear microbiome: risk factors surrounding the warfighter
Appl Environ Microbiol. 2024 Jan 3:e0117623. doi: 10.1128/aem.01176-23. Online ahead of print.ABSTRACTCombat extremity wounds are vulnerable to contamination from environments of proximity to the warfighter, leading to potential detrimental outcomes such as infection and delayed wound healing. Therefore, microbial surveillance of such environments is necessary to aid the advancement of military safety and preparedness through clinical diagnostics, treatment protocols, and uniform material design.PMID:38170999 | DOI:10.1128/aem.01176-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - January 3, 2024 Category: Microbiology Authors: Car Reen Kok Zakariae Bram James B Thissen Timothy S Horseman Keith S K Fong Susan A Reichert-Scrivner Carmen Paguirigan Kelsey O'Connor Kristina Thompson Alexander E Scheiber Shalini Mabery Viseth Ngauy Catherine F Uyehara Nicholas A Be Source Type: research

Metabolic engineering of < em > Caldicellulosiruptor bescii < /em > for 2,3-butanediol production from unpretreated lignocellulosic biomass and metabolic strategies for improving yields and titers
In this study, the thermo-cellulolytic bacterium Caldicellulosiruptor bescii was successfully engineered to produce 2,3-butanediol from cellulose, xylan, and directly from unpretreated switchgrass. Genome-scale metabolic modeling of C. bescii was applied to adjust carbon and redox fluxes to maximize productivity of 2,3-butanediol, thereby revealing bottlenecks that require genetic modifications.PMID:38131671 | DOI:10.1128/aem.01951-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - December 22, 2023 Category: Microbiology Authors: Tania N N Tanwee Gina L Lipscomb Jason L Vailionis Ke Zhang Ryan G Bing Hailey C O'Quinn Farris L Poole Ying Zhang Robert M Kelly Michael W W Adams Source Type: research

Metabolic engineering of < em > Caldicellulosiruptor bescii < /em > for 2,3-butanediol production from unpretreated lignocellulosic biomass and metabolic strategies for improving yields and titers
In this study, the thermo-cellulolytic bacterium Caldicellulosiruptor bescii was successfully engineered to produce 2,3-butanediol from cellulose, xylan, and directly from unpretreated switchgrass. Genome-scale metabolic modeling of C. bescii was applied to adjust carbon and redox fluxes to maximize productivity of 2,3-butanediol, thereby revealing bottlenecks that require genetic modifications.PMID:38131671 | DOI:10.1128/aem.01951-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - December 22, 2023 Category: Microbiology Authors: Tania N N Tanwee Gina L Lipscomb Jason L Vailionis Ke Zhang Ryan G Bing Hailey C O'Quinn Farris L Poole Ying Zhang Robert M Kelly Michael W W Adams Source Type: research

Metabolic engineering of < em > Caldicellulosiruptor bescii < /em > for 2,3-butanediol production from unpretreated lignocellulosic biomass and metabolic strategies for improving yields and titers
In this study, the thermo-cellulolytic bacterium Caldicellulosiruptor bescii was successfully engineered to produce 2,3-butanediol from cellulose, xylan, and directly from unpretreated switchgrass. Genome-scale metabolic modeling of C. bescii was applied to adjust carbon and redox fluxes to maximize productivity of 2,3-butanediol, thereby revealing bottlenecks that require genetic modifications.PMID:38131671 | DOI:10.1128/aem.01951-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - December 22, 2023 Category: Microbiology Authors: Tania N N Tanwee Gina L Lipscomb Jason L Vailionis Ke Zhang Ryan G Bing Hailey C O'Quinn Farris L Poole Ying Zhang Robert M Kelly Michael W W Adams Source Type: research

Metabolic engineering of < em > Caldicellulosiruptor bescii < /em > for 2,3-butanediol production from unpretreated lignocellulosic biomass and metabolic strategies for improving yields and titers
In this study, the thermo-cellulolytic bacterium Caldicellulosiruptor bescii was successfully engineered to produce 2,3-butanediol from cellulose, xylan, and directly from unpretreated switchgrass. Genome-scale metabolic modeling of C. bescii was applied to adjust carbon and redox fluxes to maximize productivity of 2,3-butanediol, thereby revealing bottlenecks that require genetic modifications.PMID:38131671 | DOI:10.1128/aem.01951-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - December 22, 2023 Category: Microbiology Authors: Tania N N Tanwee Gina L Lipscomb Jason L Vailionis Ke Zhang Ryan G Bing Hailey C O'Quinn Farris L Poole Ying Zhang Robert M Kelly Michael W W Adams Source Type: research

Metabolic engineering of < em > Caldicellulosiruptor bescii < /em > for 2,3-butanediol production from unpretreated lignocellulosic biomass and metabolic strategies for improving yields and titers
In this study, the thermo-cellulolytic bacterium Caldicellulosiruptor bescii was successfully engineered to produce 2,3-butanediol from cellulose, xylan, and directly from unpretreated switchgrass. Genome-scale metabolic modeling of C. bescii was applied to adjust carbon and redox fluxes to maximize productivity of 2,3-butanediol, thereby revealing bottlenecks that require genetic modifications.PMID:38131671 | DOI:10.1128/aem.01951-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - December 22, 2023 Category: Microbiology Authors: Tania N N Tanwee Gina L Lipscomb Jason L Vailionis Ke Zhang Ryan G Bing Hailey C O'Quinn Farris L Poole Ying Zhang Robert M Kelly Michael W W Adams Source Type: research

Metabolic engineering of < em > Caldicellulosiruptor bescii < /em > for 2,3-butanediol production from unpretreated lignocellulosic biomass and metabolic strategies for improving yields and titers
In this study, the thermo-cellulolytic bacterium Caldicellulosiruptor bescii was successfully engineered to produce 2,3-butanediol from cellulose, xylan, and directly from unpretreated switchgrass. Genome-scale metabolic modeling of C. bescii was applied to adjust carbon and redox fluxes to maximize productivity of 2,3-butanediol, thereby revealing bottlenecks that require genetic modifications.PMID:38131671 | DOI:10.1128/aem.01951-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - December 22, 2023 Category: Microbiology Authors: Tania N N Tanwee Gina L Lipscomb Jason L Vailionis Ke Zhang Ryan G Bing Hailey C O'Quinn Farris L Poole Ying Zhang Robert M Kelly Michael W W Adams Source Type: research