Applied and Environmental Microbiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

A new ROS response factor YvmB protects Bacillus licheniformis against oxidative stress under adverse environment
Appl Environ Microbiol. 2024 Jan 9:e0146823. doi: 10.1128/aem.01468-23. Online ahead of print.ABSTRACTUnder environment stress, the electron transfer chain will be perturbed resulting in the accumulation of H2O2 and rapidly transform to ·OH through Fenton Reaction. How do bacteria deal with oxidative stress? At present, several iron chelators have been reported to decrease the ·OH generation by sequestrating iron, while how bacteria control the synthesis of iron chelators to resist oxidative stress is still unclear. Our study found that the synthesis of iron chelator PA is induced by reactive oxygen species (ROS), which ...
Source: Applied and Environmental Microbiology - January 9, 2024 Category: Microbiology Authors: Penghui He Shiying Hu Yongjia Zhang Zhengwei Xiang Zheng Zhang Dong Wang Shouwen Chen Source Type: research

Natural transformation-specific DprA coordinate DNA double-strand break repair pathways in heavily irradiated D. radiodurans 
Appl Environ Microbiol. 2024 Jan 9:e0194823. doi: 10.1128/aem.01948-23. Online ahead of print.ABSTRACTDeinococcus radiodurans exhibits an extraordinary ability to endure and thrive in extreme environments, including exposure to radiation, desiccation, and damaging chemicals, as well as intense UV radiation. The bacterium has evolved highly efficient repair mechanisms capable of rapidly mending hundreds of DNA fragments in its genome. Our research indicates that natural transformation (NT)-specific dprA genes play a pivotal role in regulating DNA repair in response to radiation. Remarkably, we found that DprA is instrumenta...
Source: Applied and Environmental Microbiology - January 9, 2024 Category: Microbiology Authors: Dhirendra Kumar Sharma Ishu Soni Hari S Misra Yogendra Singh Rajpurohit Source Type: research

The critical roles of propanethiol oxidoreductase and sulfide-quinone oxidoreductase in the propanethiol catabolism pathway in Pseudomonas putida S-1
Appl Environ Microbiol. 2024 Jan 9:e0195923. doi: 10.1128/aem.01959-23. Online ahead of print.ABSTRACTThis work investigated the PT catabolism pathway in Pseudomonas putida S-1, a microorganism capable of utilizing PT as the sole carbon source. Critical genes that control the initiation of PT degradation were identified and characterized, such as pto and sqor. By increasing the transcription level of pto and sqor genes in vivo, we have successfully enhanced the PT degradation efficiency and growth rate of P. putida S-1. This work does not only reveal a unique PT degradation pathway but also highlights the potential of enha...
Source: Applied and Environmental Microbiology - January 9, 2024 Category: Microbiology Authors: Pei Qiao Lixiao Ning Jinhui Chen Yuhang Tang Rui Zhao Guoqing Chen Qun Ye Tong Zhou Jianmeng Chen Weihong Zhong Source Type: research

Correction for Tuntevski et al., " Aspergillus Collagen-Like Genes ( acl ): Identification, Sequence Polymorphism, and Assessment for PCR-Based Pathogen Detection"
Appl Environ Microbiol. 2024 Jan 9:e0190123. doi: 10.1128/aem.01901-23. Online ahead of print.NO ABSTRACTPMID:38193682 | DOI:10.1128/aem.01901-23 (Source: Applied and Environmental Microbiology)
Source: Applied and Environmental Microbiology - January 9, 2024 Category: Microbiology Authors: Kiril Tuntevski Brandon C Durney Ann а K Snyder P Rocco LaSala Ajay P Nayak Brett J Green Donald H Beezhold Rita V M Rio Lisa A Holland Slawomir Lukomski Source Type: research

Seasonal influenza viruses decay more rapidly at intermediate humidity in droplets containing saliva compared to respiratory mucus
Appl Environ Microbiol. 2024 Jan 9:e0201023. doi: 10.1128/aem.02010-23. Online ahead of print.ABSTRACTDetermining how long viruses persist in the environment is important for mitigating transmission risk. Expelled infectious droplets and aerosols are composed of respiratory fluids, including saliva and complex mucus mixtures, but how well influenza viruses survive in such fluids is largely unknown. Here, we find that infectious influenza virus is present in the oral cavity of infected ferrets, suggesting that saliva-containing expulsions can play a role in onward transmission. Additionally, influenza virus in droplets comp...
Source: Applied and Environmental Microbiology - January 9, 2024 Category: Microbiology Authors: Nicole C Rockey Valerie Le Sage Linsey C Marr Seema S Lakdawala Source Type: research

Use of CRISPR interference for efficient and rapid gene inactivation in Fusobacterium nucleatum 
Appl Environ Microbiol. 2024 Jan 8:e0166523. doi: 10.1128/aem.01665-23. Online ahead of print.ABSTRACTHow can we effectively investigate the gene functions in Fusobacterium nucleatum, given the dual challenges of gene inactivation and the inherent genetic resistance of many strains? Traditional methods have been cumbersome and often inadequate. Addressing this, our work introduces a novel inducible CRISPR interference (CRISPRi) system in which dCas9 expression is controlled at the translation level by a theophylline-responsive riboswitch unit, and single-guide RNA expression is driven by the robust, constitutive rpsJ promo...
Source: Applied and Environmental Microbiology - January 8, 2024 Category: Microbiology Authors: Peng Zhou Bibek G C Flynn Stolte Chenggang Wu Source Type: research

Microbial associates of the elm leaf beetle: uncovering the absence of resident bacteria and the influence of fungi on insect performance
Appl Environ Microbiol. 2024 Jan 5:e0105723. doi: 10.1128/aem.01057-23. Online ahead of print.ABSTRACTOur study provides insights into the still understudied role of microbial symbionts in the biology of the elm leaf beetle (ELB), a major pest of elms. Contrary to expectations, we found no persistent bacterial symbionts associated with the ELB or elm leaves. Our research thus contributes to the growing body of knowledge that not all insects rely on bacterial symbionts. While no persistent bacterial symbionts were detectable in the ELB and elm leaf samples, our analyses revealed the persistent presence of fungi, particularl...
Source: Applied and Environmental Microbiology - January 5, 2024 Category: Microbiology Authors: Johanna Schott Juliette Rakei Mitja Remus-Emsermann Paul Johnston Susan Mbedi Sarah Sparmann Monika Hilker Luis R Paniagua Voirol Source Type: research

Polysaccharide utilization loci from Bacteroidota encode CE15 enzymes with possible roles in cleaving pectin-lignin bonds
Appl Environ Microbiol. 2024 Jan 5:e0176823. doi: 10.1128/aem.01768-23. Online ahead of print.ABSTRACTThe plant cell wall is a highly complex matrix, and while most of its polymers interact non-covalently, there are also covalent bonds between lignin and carbohydrates. Bonds between xylan and lignin are known, such as the glucuronoyl ester bonds that are cleavable by CE15 enzymes. Our work here indicates that enzymes from CE15 may also have other activities, as we have discovered enzymes in PULs proposed to target other polysaccharides, including pectin. Our study represents the first investigation of such enzymes. Our fir...
Source: Applied and Environmental Microbiology - January 5, 2024 Category: Microbiology Authors: Andrea Seveso Scott Mazurkewich Sanchari Banerjee Jens-Christian Navarro Poulsen Leila Lo Leggio Johan Larsbrink Source Type: research

Evolution and separation of actinobacterial pyranose and C -glycoside-3-oxidases
Appl Environ Microbiol. 2024 Jan 5:e0167623. doi: 10.1128/aem.01676-23. Online ahead of print.ABSTRACTC-Glycosides often form active compounds in various plants. Breakage of the C-C bond in these glycosides to release the aglycone is challenging and proceeds via a two-step reaction, the oxidation of the sugar and subsequent cleavage of the C-C bond. Recently, an enzyme from a soil bacterium, FAD-dependent C-glycoside-3-oxidase (CGOx), was shown to catalyze the initial oxidation reaction. Here, we show that CGOx belongs to the same sequence space as pyranose oxidase (POx), and that an actinobacterial ancestor of the POx/CGO...
Source: Applied and Environmental Microbiology - January 5, 2024 Category: Microbiology Authors: Anja Kostelac Andr é Taborda L ígia O Martins Dietmar Haltrich Source Type: research