In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay

In this study, we designed a chemiluminescence dot blot assay to detect the 3A and 3B NSPs of FMDV. It is sensitive enough to detect up to 20 ng of the NSP, and exhibited specificity as it does not react with the viral SPs. This cost-effective assay holds promise in quality control assessment in FMD vaccine manufacturing.PMID:38479084 | DOI:10.1016/j.jviromet.2024.114906

Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Uzma Jabeen Kailash Singh Bisht Huildore Bommanna Ranjitha Madhusudan Hosamani Beeragere Parameshwaraiah Sreenivasa Pratik M Kulkarni Dombesara Chandrashekar Nidhi Rajegowdanadoddi Lakshmana Amulya Veerakyathappa Bhanuprakash Hosur Joyappa Dechamma Aniket Source Type: research