Microbial Contamination and Disinfection of Sport Mouthguard: In Vitro Study.
In conclusion, hydrogen peroxide, 0.5% sodium hypochlorite and the solution Oral Care Foam™ allowed to obtain an optimum disinfection of the mouthguard. SEM observation showed that different substances demonstrated a decontaminating effect decreasing the microbial communities on the EVA surface. PMID: 31813013 [PubMed - as supplied by publisher] (Source: Current Microbiology)
Source: Current Microbiology - December 7, 2019 Category: Microbiology Authors: D'Ercole S, Tieri M, Martinelli D, Ciaravino C, Fulco D, Tripodi D Tags: Curr Microbiol Source Type: research

Key Factors for Removing Bias in Viability PCR-Based Methods: A Review.
te; G Abstract During the last decade, the viability PCR approach has been investigated intensively with the goal of detecting solely the DNA derived from intact cells by removing the bias resulting from dead or damaged cell artifacts. However, since the first published paper in 2003 and despite the various continuous improvements, the stubborn reality is that in most cases the goal has not been achieved. Complete neutralization of nucleic acids derived from dead microorganisms was not possible, at least without affecting live cells. However, recently published works in this field show how neutralization rates can...
Source: Current Microbiology - December 6, 2019 Category: Microbiology Authors: Codony F, Dinh-Thanh M, Agustí G Tags: Curr Microbiol Source Type: research

Pararcticibacter amylolyticus gen. nov., sp. nov., Isolated from a Rotten Hemp Rope, and Reclassification of Pedobacter tournemirensis as Pararcticibacter tournemirensis comb. nov.
Abstract A Gram-stain-negative, rod-shaped, non-motile, facultatively anaerobic bacterium, designated FJ4-8T, was isolated from a rotten hemp rope in Chongqing City, PR China. Phylogenetic analysis of 16S rRNA gene sequences indicated that the isolate was closely related to members of the family Sphingobacteriaceae, with the highest similarity to Pedobacter tournemirensis TF5-37.2-LB10T (95.3%) and low similarities to all other species of the genus Pedobacter (90.4-93.9%). Phylogenetic analyses demonstrated that strain FJ4-8T formed a stable subclade with Pedobacter tournemirensis TF5-37.2-LB10T. The clade with th...
Source: Current Microbiology - December 6, 2019 Category: Microbiology Authors: Cai YY, Dong WW, Hu YL, Jiang X, Wang YW, Zhou YQ, Peng N, Liang YX, Zhao SM Tags: Curr Microbiol Source Type: research

Effect of Black Grape Seed Extract (Vitis vinifera) on Biofilm Formation of Methicillin-Resistant Staphylococcus aureus and Staphylococcus haemolyticus.
Abstract Grape seeds are considered one of the most important sources for phenolic and other compounds and is globally consumed for the biological value of its active ingredients. The increasing prevalence of Methicillin-resistant Staphylococcus aureus-related infections has become a very challenging health issue worldwide. This work aims at examining the antibacterial activity of alcoholic extract of black grape seeds (Vitis vinifera) against biofilm formation by Staphylococcus aureus and Staphylococcus haemolyticus. Staphylococcal bacterial isolates were first clinically confirmed using the VITEK-2compact system...
Source: Current Microbiology - December 5, 2019 Category: Microbiology Authors: Al-Mousawi AH, Al-Kaabi SJ, Albaghdadi AJH, Almulla AF, Raheem A, Algon AAA Tags: Curr Microbiol Source Type: research

Aureimonas leprariae sp. nov., Isolated from a Lepraria sp. Lichen.
Abstract A Gram-negative, motile, aerobic and coccoid rod-shaped bacterium, designated strain YIM132180T, was isolated from a Lepraria sp. lichen collected from Pu'er, Yunnan Province, China. The strain grew at 15-35 °C (optimum, 25-28 °C), at 0-2% (w/v) NaCl (optimum, 0-1%) and at pH 6.0-9.0 (optimum, pH 7.0). The 16S rRNA gene sequence showed that strain YIM132180T had highest similarity (96.4%) with Aureimonas endophytica 2T4P-2-4T, followed by Aureimonas ureilytica NBRC 106430T (95.7%) and Aureimonas rubiginis CC-CFT034T (95.6%). Phylogenetic analysis showed that the strain grouped with speci...
Source: Current Microbiology - December 4, 2019 Category: Microbiology Authors: Zhang K, Jiang LQ, Wang LS, An DF, Lang L, Li GD, Wang XY, Shi SB, Li QY, Jiang CL, Jiang Y Tags: Curr Microbiol Source Type: research

Mitochondrial Citrate Transport System in the Fungus Mucor circinelloides: Identification, Phylogenetic Analysis, and Expression Profiling During Growth and Lipid Accumulation.
Abstract The mitochondrial citrate transport system, composed of citrate and malate transporters (MTs), can regulate the citrate efflux from mitochondria to cytosol, and then citrate is cleaved into OAA and acetyl-CoA which can be used for fatty acid (FA) biosynthesis. However, in the fungus Mucor circinelloides the molecular mechanism of citrate efflux from the mitochondria by this system and its role in FA synthesis is unclear. In the present study, we have analyzed the genome of high lipid-producing strain WJ11 and the low lipid-producing strain CBS 277.49 to find the potential genes involving in this system. F...
Source: Current Microbiology - December 4, 2019 Category: Microbiology Authors: Yang J, Khan MAK, Zhang H, Zhang Y, Certik M, Garre V, Song Y Tags: Curr Microbiol Source Type: research

A Novel PCR-Based Approach for Rapid Identification of Morchella sextelata Using Species-Specific Primers.
In this study, a PCR-based approach was developed for identification of Morchella sextelata, based on a 190-bp segment of internal transcribed spacer (ITS). The species-specific primer, MS1F/MS1R, was designed based on the alignment of sequence obtained from the 20 representative cultivated body fruit of Morchella sextelata as well as 10 sequence data available in Genbank. Verifying the species-specific primer pair demonstrated generation of an amplicon from extracted DNA of Morchella sextelata. The primer pair was also applied and tested for five other species of morels and eight common mushrooms from other genus. This no...
Source: Current Microbiology - December 4, 2019 Category: Microbiology Authors: Shen Q, Li C, Xie M, Li W, Qian Z, Zhang J Tags: Curr Microbiol Source Type: research

Chryseotalea sanaruensis gen. nov., sp., nov., a Member of the Family Cytophagaceae, Isolated from a Brackish Lake in Hamamatsu Japan.
Abstract A strain-designated YsT was isolated as a filterable bacterial strain from Lake Sanaru, a brackish water lake in Hamamatsu Japan. YsT is aerobic, Gram-negative, and slender rod shaped. YsT grew optimally at 30 °C, pH 7.0-8.0 and without the addition of NaCl. MK-7 was the sole isoprenoid quinone. The main cellular polar lipids were phosphatidylethanolamine and unidentified amino- and polar-lipids. The predominant cellular fatty acids were C18:0, iso-C14:0 and iso-C15:0. Phylogenetic analysis of 16S rRNA gene sequence revealed the nearest neighbours of strain YsT to be members of the Ohtaekwangia a...
Source: Current Microbiology - December 2, 2019 Category: Microbiology Authors: Maejima Y, Iino T, Muraguchi Y, Fukuda K, Ohkuma M, Suzuki T, Moriuchi R, Dohra H, Kimbara K, Shintani M Tags: Curr Microbiol Source Type: research

Impact of Spray-Drying on Biological Properties of Chitosan Matrices Supplemented with Antioxidant Fungal Extracts for Wine Applications.
In this study, crude antioxidant extracts obtained from A. tubingensis G131 cultures were encapsulated with two types of chitosan matrix. Spray-drying was used to obtain dried particles from a dispersion of fungal crude extracts in a solution of the coating agent chitosan. This process appeared to be an efficient method for obtaining a dry extract with antioxidant activity. Three types of fungal extracts, with different antioxidant capacities, were produced: two different concentrations of crude extract and a semi-purified extract. In this study, the chitosan matrices for encapsulation were chosen on the basis of their ant...
Source: Current Microbiology - November 30, 2019 Category: Microbiology Authors: Choque E, Durrieu V, Alric I, Raynal J, Mathieu F Tags: Curr Microbiol Source Type: research

Edaphocola flava sp. nov., Isolated from Herbicide Bensulfuron-methyl-Contaminated Soil.
Abstract A novel strain, HME-24T, was isolated from herbicide bensulfuron-methyl-contaminated soil in Jiangning District, Jiangsu Province, China. Strain HME-24T was a Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterium, and the colonies on R2A agar are yellow. The strain was non-sporulating, catalase- and oxidase-positive. Cells growth occurred at 15-37 °C (optimum, 30 °C), at pH 6.0-8.0 (optimum, 7.0) and with 0-1.5% (w/v) NaCl (optimum, 0.5% NaCl). Strain HME-24T showed the highest 16S rRNA gene sequence identity to Edaphocola aurantiacus H2T (99.58%), followed by Taibaiella chish...
Source: Current Microbiology - November 30, 2019 Category: Microbiology Authors: Hu T, Xiang Y, Xing ZY, Qi P, Huang X Tags: Curr Microbiol Source Type: research

Anti-biofilm Effect of β-Lapachone and Lapachol Oxime Against Isolates of Staphylococcus aureus.
Anti-biofilm Effect of β-Lapachone and Lapachol Oxime Against Isolates of Staphylococcus aureus. Curr Microbiol. 2019 Nov 29;: Authors: Fernandes AWC, Dos Anjos Santos VL, Araújo CRM, de Oliveira HP, da Costa MM Abstract Antimicrobial resistance in bacteria, such as Staphylococcus aureus, has been the subject of many assistance studies of alternatives for the treatment of infections. These studies aim to solve this problem for bacteria, such as biofilm formation. Aiming to control the emergence of the problem or enhance antibiotic activity, the data sources are inserted into new therapeuti...
Source: Current Microbiology - November 29, 2019 Category: Microbiology Authors: Fernandes AWC, Dos Anjos Santos VL, Araújo CRM, de Oliveira HP, da Costa MM Tags: Curr Microbiol Source Type: research

Flagellimonas algicola sp. nov., Isolated from a Marine Red Alga, Asparagopsis taxiformis.
Abstract A Gram-stain-negative, strictly aerobic and moderately halophilic bacterium, designated strain AsT0115T, was isolated from a red marine alga, Asparagopsis taxiformis. Cells were non-motile rods showing oxidase-positive and catalase-negative activities. Growth was observed at 15-37 C (optimum, 25 C) and pH 6.5-7.5 (optimum, pH 7.0) and in the presence of 0-11.0% (w/v) NaCl (optimum, 2.0-3.0%). Strain AsT0115T contained iso-C15:0 and iso-C15:1 G as the predominant fatty acids. Menaquinone-6 was identified as the sole isoprenoid quinone. Major polar lipids consisted of phosphatidylethanolamine, an ...
Source: Current Microbiology - November 28, 2019 Category: Microbiology Authors: Kim J, Kim KH, Chun BH, Khan SA, Jeon CO Tags: Curr Microbiol Source Type: research

Growth Pattern of Magnetic Field-Treated Bacteria.
Abstract A study of the induced effect of different types of weak magnetic field exposure on bacterial growth is performed, comparing the relative changes after removal from the magnetic fields. This investigation is relevant to understand the effect of magnetic field exposure on human beings due to electronic devices. For this purpose, we use four species of common bacteria in reference to human health and safety including Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The choice of these four bacteria also allows us to check for effects which rely upon the Gram-s...
Source: Current Microbiology - November 27, 2019 Category: Microbiology Authors: Masood S, Saleem I, Smith D, Chu WK Tags: Curr Microbiol Source Type: research

Tinea Gladiatorum and Dermatophyte Contamination Among Wrestlers and in Wrestling Halls: A Systematic Review and Meta-analysis.
Abstract To present an overview of the existing epidemiological evidence regarding the occurrence of tinea gladiatorum in wrestlers and dermatophyte contamination in wrestling halls, five electronic databases including "PubMed," "Scopus," "Google Scholar," "Science Direct," and "Web of Science" were searched from inception to 30 December 2018. Studies focusing on epidemiology of dermatophytosis among wrestlers and on the presence of dermatophytes in wrestler hall were included. Data from 13 studies, 4818 wrestlers, and 391 wrestling mat samples from Turkey, Iran, a...
Source: Current Microbiology - November 26, 2019 Category: Microbiology Authors: Kermani F, Moosazadeh M, Hosseini SA, Bandalizadeh Z, Barzegari S, Shokohi T Tags: Curr Microbiol Source Type: research

Isolation and Complete Genome of the Marine Pseudoalteromonas Phage C7 from Coastal Seawater of Yellow Sea, China.
This study provides an important data for further research on the interaction between marine bacteriophages and their hosts. PMID: 31760453 [PubMed - as supplied by publisher] (Source: Current Microbiology)
Source: Current Microbiology - November 23, 2019 Category: Microbiology Authors: Wang Q, Liu Y, Liu Q, Liu X, Yang F, Wang M, Shao H, Jiang Y Tags: Curr Microbiol Source Type: research

Mobile Genetic Elements in Pseudomonas stutzeri.
Abstract Mobile genetic elements (MGE) play a large role in the plasticity of genomes, participating in several phenomena which involve genes acquisition. Pseudomonas stutzeri is an environmental widely distributed bacteria. This bacteria has a very large genomic plasticity, which would explain its occurrence in several different environments. NCBI data bank and online programs were used to build an inventory to investigate diversity and structure of MGE in Pseudomonas stutzeri, searching for insertion sequences (IS), integrases/transposases, plasmids and prophages. Five hundred and forty-eight ISs, 62 integrases,...
Source: Current Microbiology - November 21, 2019 Category: Microbiology Authors: de Sousa LP Tags: Curr Microbiol Source Type: research

Phage Transduction is Involved in the Intergeneric Spread of Antibiotic Resistance-Associated blaCTX-M, mel, and tetM Loci in Natural Populations of Some Human and Animal Bacterial Pathogens.
In this study, we examined the genome sequences of 2239 bacteriophages from different sources for the presence of ARGs. The identified ARG-carrying bacteriophages were then analyzed by PHACTS, PHAST, and HostPhinder programs to determine their lifestyles, genes coding for bacterial cell lysis, recombinases, and a spectrum of their potential host species, respectively. We employed the SplitsTree, RDP4 and SimPlot software packages in recombination tests to identify HGT events of ARGs between these bacteriophages and bacteria. In our analyses, some ARG-carrying bacteriophages exhibited temperate and/or polyvalent patterns. T...
Source: Current Microbiology - November 21, 2019 Category: Microbiology Authors: Gabashvili E, Osepashvili M, Koulouris S, Ujmajuridze L, Tskhitishvili Z, Kotetishvili M Tags: Curr Microbiol Source Type: research

Geomonas soli gen. nov., sp. nov., a New Member of the Family Comamonadaceae, Isolated from Soil.
Abstract A Gram-stain negative, facultative aerobic bacterial strain, designated strain S-16T, was isolated from soil in South Korea. Colonies were white-milkish and cells were non-motile rods with oxidase- and catalase-positive activities. The growth of strain S-16T was observed at 20-40 °C (optimum, 25-30 °C) and pH 5.5-7.0 (optimum, pH 6.5). Ubiquinone-8 was identified as the sole respiratory quinone and C12:0, C16:0, C18:0, C15:1ω5c and summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c) were identified as the major fatty acids (>5%). The major polar lipids consisted o...
Source: Current Microbiology - November 21, 2019 Category: Microbiology Authors: Khan SA, Jeong SE, Park HY, Lee SS, Jeon CO Tags: Curr Microbiol Source Type: research

Characterization and Genome Analysis of a Zearalenone-Degrading Bacillus velezensis Strain ANSB01E.
Abstract Zearalenone, a nonsteroidal estrogenic mycotoxin mainly produced by Fusarium species, causes reproductive disorders and hyperestrogenic syndromes in animals and humans. The bacterial strain Bacillus velezensis ANSB01E, isolated from chicken cecal content, was capable of effectively degrading zearalenone in both liquid medium and mouldy corn. Moreover, Bacillus velezensis ANSB01E exhibited good antimicrobial activities against animal pathogenic bacteria, including Escherichia coli, Staphylococcus aureus, and Salmonella spp. Genome-based analysis revealed the presence of genes coding peroxiredoxin and alpha...
Source: Current Microbiology - November 20, 2019 Category: Microbiology Authors: Guo Y, Zhou J, Tang Y, Ma Q, Zhang J, Ji C, Zhao L Tags: Curr Microbiol Source Type: research

Enzymatic Characteristics of a Polyphosphate/ATP-NAD Kinase, PanK, from Myxococcus xanthus.
Abstract NAD kinase is a crucial enzyme for production of NADP+. Myxococcus xanthus is a gram-negative soil bacterium that forms fruiting bodies and spores under starvation, and it accumulates polyphosphate (poly(P)) during early development. We found that M. xanthus NAD kinase (PanK) utilized both ATP and poly(P) as phosphoryl donors; therefore, PanK was designated as a poly(P)/ATP-NAD kinase. Unlike other poly(P)/ATP-NAD kinases, PanK hardly exhibited NADH kinase activity. The NAD kinase activity of PanK was inhibited by NADPH, but not NADH. Replacement of Thr-90 in the GGDGT motif of PanK with Asn decreased bot...
Source: Current Microbiology - November 18, 2019 Category: Microbiology Authors: Kimura Y, Kamimoto T, Tanaka N Tags: Curr Microbiol Source Type: research

Evaluation of eazyplex ® SuperBug CRE Test for Beta-Lactamase Genes Detection in Klebsiella spp. and P. aeruginosa Strains.
Evaluation of eazyplex® SuperBug CRE Test for Beta-Lactamase Genes Detection in Klebsiella spp. and P. aeruginosa Strains. Curr Microbiol. 2019 Nov 14;: Authors: Sękowska A, Bogiel T, Gospodarek-Komkowska E Abstract The multi-drug resistance of Gram-negative rods is one of the most important issues of present medicine. In recent years, more and more strains resistant to the majority or to all possible therapeutic options have been isolated-especially Klebsiella spp. and Pseudomonas spp. representatives. It is very important to detect strains with these phenotypes as quickly and reliably as possib...
Source: Current Microbiology - November 14, 2019 Category: Microbiology Authors: Sękowska A, Bogiel T, Gospodarek-Komkowska E Tags: Curr Microbiol Source Type: research

The Resurgence of Dirigent Story: Time for a Bacterial Chapter.
Abstract For several decades, dirigent (DIR) domain-containing proteins have been assumed to be green lineage-specific, responsible for the defence response and lignan/lignin biosynthesis. Despite their high potential in terms of biotechnology and chemistry, to date there have been very few well-studied plant DIRs. However, recent achievements in sequencing technologies have allowed for discovery of DIR genes in bacteria. This prospective study suggests expansion of the focus of research to consider the existence of bacterial DIRs. It also considers the outlook for understanding DIR functioning with respect to the...
Source: Current Microbiology - November 14, 2019 Category: Microbiology Authors: Dabravolski SA Tags: Curr Microbiol Source Type: research

Lysobacter segetis sp. nov., Isolated from Soil.
Abstract A Gram-negative, aerobic, motile by gliding, rod-shaped bacterium, strain 17J68-2T, was isolated from a soil sample taken from Jeju Island, Republic of Korea. The isolate displayed high 16S rRNA gene sequence similarity to the members of the genus Lysobacter in the family Lysobacteraceae, with Lysobacter humi FJY8T (98.4% similarity), Lysobacter xinjiangensis RCML-52T (98.3%), and Lysobacter mobilis 9NM-14T (98.1%) as closest phylogenetic neighbors. Growth of strain 17J68-2T occurred at 15-42 °C, pH 7-8, and in the presence of 0-1.0% NaCl. Draft genome was 2.94 Mb in size with G+C content of...
Source: Current Microbiology - November 14, 2019 Category: Microbiology Authors: Ten LN, Jeon J, Elderiny NS, Kim MK, Lee SY, Jung HY Tags: Curr Microbiol Source Type: research

Enhancement of Purified Human Colon Cancer-Specific Parasporal Toxin from Bacillus thuringiensis-LDC-501.
Abstract Parasporal inclusion protein of Bacillus thuringiensis-LDC-501 (Bt-LDC-501) exhibits selective cytocidal action towards human colon cancer cells. The yield of this parasporal protein was minimum in the normal culture. In order to increase the yield of protein from Bt-LDC-501 various agro-based cost-efficient nutrient sources such as corn steep liquor (CSL), sesame oil cake extract (SOC), groundnut oil cake extract (GOC), neem oil cake extract (NOC), rice bran extract (RB), wheat bran extract (WB), red gram hull extract (RGH), green gram hull extract (GGH), black gram hull extract (BGH), Mysore gram hull e...
Source: Current Microbiology - November 14, 2019 Category: Microbiology Authors: Grace JJ, Ramani G, Shenbagarathai R Tags: Curr Microbiol Source Type: research

Multiplexed Non-barcoded Long-Read Sequencing and Assembling Genomes of Bacillus Strains in Error-Free Simulations.
Abstract The generation of genomic data from microorganisms has revolutionized our abilities to understand their biology, but it is still challenging to obtain complete genome sequences of microbes in an automated high-throughput and cost-effective manner. While the advent of second-generation sequencing technologies provided significantly higher throughput, their shorter lengths and more pronounced sequence-context bias led to a shift towards resequencing applications. Recently, single molecule real-time (SMRT) DNA sequencing has been used to generate sequencing reads that are much longer than other sequencing pl...
Source: Current Microbiology - November 13, 2019 Category: Microbiology Authors: Qian J, Meng Q, Feng Y, Mao X, Ling Y, Li J Tags: Curr Microbiol Source Type: research

Induction of Systemic Resistance in Chickpea (Cicer arietinum L.) Against Fusarium oxysporum f. sp. ciceris by Antagonistic Rhizobacteria in Assistance with Native Mesorhizobium.
Abstract In the present study five potent rhizobacterial antagonists of Fusarium oxysporum f. sp. ciceris alone and in combination with Mesorhizobium (M) were evaluated for their potential to elicit the defence response reactions to reduce the total loss of plants and enhance the growth of two chickpea cultivars i.e. resistant GPF-2 and susceptible JG-41. Observations revealed that maximum phenolic, peroxidase (PO) and polyphenol oxidase (PPO) activity was induced after 30th day of germination. Maximum phenol concentration of 745.8 and 724.1 μg/gfw root tissues was recorded by Ps45 when co-inoculated with ...
Source: Current Microbiology - November 13, 2019 Category: Microbiology Authors: Kumari S, Khanna V Tags: Curr Microbiol Source Type: research

Genome Shuffling of Bacillus velezensis for Enhanced Surfactin Production and Variation Analysis.
In this study, genome shuffling was employed to improve surfactin production in Bacillus velezensis strain LM3403 via recursive protoplast fusion. RT-qPCR analysis was employed to evaluate the transcriptional variance of surfactin synthase genes and surfactin efflux gene to insight into the variance underlying the recombinant strain. After three rounds of genome shuffling, a high-yield and genetic stable recombinant F34 was obtained, exhibiting dramatic improvement in surfactin production (from 229.60 ± 7.10 mg/L to 908.15 ± 5.65 mg/L) with high proportion of long carbon ...
Source: Current Microbiology - November 12, 2019 Category: Microbiology Authors: Chen L, Chong XY, Zhang YY, Lv YY, Hu YS Tags: Curr Microbiol Source Type: research

Pseudomonas jilinensis sp. nov., Isolated from Oil Production Water of Jilin Oilfield in China.
Abstract A Gram-stain-negative, rod-shaped bacterial strain JS15-10A1T was isolated from oil production water. Its optimum growth was observed at 37 °C, pH 7.0, and 3% (w/v) NaCl. The 16S rRNA gene sequence of strain JS15-10A1T showed the highest similarities with Pseudomonas parafulva CB-1T (97.6%) and P. fulva IAM 1529T (97.5%). In addition, phylogenetic analyses based on multilocus sequence analyses with concatenating 16S rRNA, gyrB, rpoD, and rpoB genes indicated that strain JS15-10A1T was a member of genus Pseudomonas but discriminated from other species. Furthermore, whole-genome analyses revealed t...
Source: Current Microbiology - November 11, 2019 Category: Microbiology Authors: Wang JW, Cai M, Nie Y, Hu B, Yang Y, Wu XL Tags: Curr Microbiol Source Type: research

Intracellular Proteomic Analysis of Streptomyces sp. MC1 When Exposed to Cr(VI) by Gel-Based and Gel-Free Methods.
as LB Abstract The actinobacterium Streptomyces sp. MC1 has previously shown the capacity to resist and remove Cr(VI) from liquid culture media. The aim of this work is to analyze the differential expression pattern of intracellular proteins when Streptomyces sp. MC1 is exposed to Cr(VI) in order to explain the molecular mechanisms of resistance that this microorganism possesses. For this purpose, 2D-PAGE and shotgun proteomic analyses (2D-nanoUPLC-ESI-MS/MS) were applied. The presence of Cr(VI) induced the expression of proteins involved in molecular biosynthesis and energy generation, chaperones with a key role ...
Source: Current Microbiology - November 9, 2019 Category: Microbiology Authors: Bonilla JO, Callegari EA, Estevéz MC, Villegas LB Tags: Curr Microbiol Source Type: research

Regulation of Pyruvate Formate Lyase-Deficient Klebsiella pneumoniae for Efficient 1,3-Propanediol Bioproduction.
Abstract Anaerobic growth defect of pyruvate formate lyase (PFL)-deficient Klebsiella pneumoniae limits its industrial application, and the reason for this growth defect was analyzed in this study. The obtained evidences, combined with normal intracellular redox status and no further inhibition by adhE deletion, strongly suggested that growth defect in PFL-deficient K. pneumoniae was probably caused by lack of carbon flux from pyruvate to acetyl-CoA (AcCoA). Correspondingly, the anaerobic growth of PFL-deficient K. pneumoniae was promoted by deletion of pdhR, a negative transcriptional regulator gene for AcCoA gen...
Source: Current Microbiology - November 8, 2019 Category: Microbiology Authors: Bao W, Wei R, Liu X, Dong S, Chen T, Fu S, Gong H Tags: Curr Microbiol Source Type: research

Rheinheimera pleomorphica sp. nov., a Novel Alkali-Tolerant Bacteria Isolated from Chilika Lake, India.
Abstract A novel Gram-negative gamma-proteobacterium, non-sporulating motile, rod or coccus-shaped bacterium designated as strain PKS7T was isolated from a sediment sample collected from Chilika Lake, Odisha, India and characterized taxonomically using a polyphasic approach. The major quinone was Q8 and major cellular fatty acids were C16:0, C17:0, C15:1w8c, C17:1w8c, C12:03-OH. The chemotaxonomic features confirmed the isolate to be a member of genus Rheinheimera. 16SrRNA gene sequence of strain PKS7T was closest in similarity to R. aquimaris SW-353T (99.36% identity), R. muenzenbergensis E49T (98.63%), R. nanhai...
Source: Current Microbiology - November 8, 2019 Category: Microbiology Authors: Panda AN, Ray L, Mishra SR, Raina V Tags: Curr Microbiol Source Type: research

The Genome of the Plant-Associated Lactic Acid Bacterium Lactococcus lactis KF147 Harbors a Hybrid NRPS-PKS System Conserved in Strains of the Dental Cariogenic Streptococcus mutans.
This study mainly aimed to predict the structure and function of the hybrid NRP-PK product of L. lactis KF147 using comparative genomics techniques, and included a detailed analysis of the regulatory system. Various bioinformatical approaches were used to predict the substrate specificity of the six A domains and the iterative transAT domain. Functional conservation of the A domains within different-niche-associated strains supported the prediction of the primary core structure of the putative hybrid natural product to be Leu-DLeu-Asp-DAsn-Gly-MC-MC-MC-DAsp (MC = Malonyl-CoA). Oxidative stress resistance and ...
Source: Current Microbiology - November 8, 2019 Category: Microbiology Authors: Khayatt BI, van Noort V, Siezen RJ Tags: Curr Microbiol Source Type: research

Taking a Closer Look at the Valid Publication and Authorship of Legionella bozemanae Brenner et al. 1980, Fluoribacter bozemanae Garrity et al. 1980, Legionella pittsburghensis Pasculle et al. 1980, Legionella micdadei H ébert et al. 1980 and Tatlockia micdadei (Hébert et al. 1980) Garrity et al. 1980.
Taking a Closer Look at the Valid Publication and Authorship of Legionella bozemanae Brenner et al. 1980, Fluoribacter bozemanae Garrity et al. 1980, Legionella pittsburghensis Pasculle et al. 1980, Legionella micdadei Hébert et al. 1980 and Tatlockia micdadei (Hébert et al. 1980) Garrity et al. 1980. Curr Microbiol. 2019 Nov 08;: Authors: Tindall BJ Abstract The names Legionella bozemanae Brenner et al. 1980, Fluoribacter bozemanae Garrity et al. 1980, Legionella pittsburghensis Pasculle et al. 1980, Legionella micdadei Hébert et al. 1980 and Tatlockia micdadei (Hébert et ...
Source: Current Microbiology - November 8, 2019 Category: Microbiology Authors: Authorship of Legionella bozemanae Brenner et al. 1980, Fluoribacter bozemanae Garrity et al. 1980, Legionella pittsburghensis Pasculle et al. 1980, Legionella micdadei Hébert et al. 1980 and Tatlockia micdadei (Hébert et al. 1980) Garrity et al. 1980. Tags: Curr Microbiol Source Type: research

The Role of Cross-Pathway Control Regulator CpcA in the Growth and Extracellular Enzyme Production of Penicillium oxalicum.
In this study, the biological function of CpcA in Penicillium oxalicum was investigated under different cultivation conditions. Disruption of cpcA led to decreased cell growth either in the presence or absence of histidine biosynthetic inhibitor, and the phenotype could be rescued by the addition of exogenous amino acid sources. In addition, CpcA was required for the rapid production of cellulase when cells were cultured on cellulose. Transcript abundance measurement showed that a set of amino acid biosynthetic genes as well as two major cellulase genes were significantly down-regulated in cpcA deletion mutant relative to ...
Source: Current Microbiology - November 7, 2019 Category: Microbiology Authors: Pan Y, Gao L, Zhang X, Qin Y, Liu G, Qu Y Tags: Curr Microbiol Source Type: research

Complete Genome Sequence of Strain YCSC6, a Marine Bacterium Isolated from Saturated Saltpan with Activity Against Uronema marinum.
In this study, we sequenced its complete genome. Results showed that it consists of two circular chromosomes: 2.49 Mbps and 0.74 Mbps, respectively. It encodes 3429 protein-coding sequences. Biosynthetic gene clusters predicted to synthesize bacteriocins and antimicrobial peptides were discovered, which might be the key factors to lyse and kill U. marinum. The complete genome sequence of strain YCSC6 provides insights into the fundamental genetic potential for elucidating its insecticidal mechanism against U. marinum. PMID: 31691022 [PubMed - as supplied by publisher] (Source: Current Microbiology)
Source: Current Microbiology - November 6, 2019 Category: Microbiology Authors: Du GX, Qu LY, Shang K, Gao P, Ding W, Sun CJ Tags: Curr Microbiol Source Type: research

Isolation and Genome Sequencing of a Novel Pseudomonas aeruginosa Phage PA-YS35.
Abstract Phage PA-YS35 is a novel lytic Pseudomonas aeruginosa phage belonging to the Myoviridae family and was isolated from the sewage of the First Hospital of Jilin University. The biological properties testing indicated that phage PA-YS35 is stable between - 20 and 60 °C and pH 4-9. The one-step growth curve shows that the latent period of PA-YS35 was 9 min, and the burst period was about 21 min by the size of approximately 380 progeny phages per host cell. The genome of phage PA-YS35 is linear double-stranded DNA with a size of 93,296 bp and a GC content of 49.35%. The results from RAST gene ann...
Source: Current Microbiology - October 29, 2019 Category: Microbiology Authors: Jiang YH, Liu JQ, Zhao CY, Yu S, Sun YB, Shi HY, Huang HL Tags: Curr Microbiol Source Type: research

Chitooligosaccharides and Thermostable Chitinase Against Vulvovaginal Candidiasis and Saprophyte Fungi: LC Mass Studies of Shrimp Shell Fermentation by Bacillus altitudinis.
Abstract Some patients with candidiasis seek alternatives drug to treat vaginal yeast infection like herbal preparations and probiotics. However, the effectiveness of such treatments has not received much study. In this research, the unique chitinotrophic Bacillus was isolated on shrimp shell from salt lakes and identified as Bacillus altitudinis by 16SRNA sequencing. This strain produced a novel chitin-oligosaccharide material and thermostable chitinase (5.1 units/ml) during 4 days incubation on shrimp shell medium; nevertheless, its growth on nutrient agar was negative. The zymogram showed less than 50...
Source: Current Microbiology - October 28, 2019 Category: Microbiology Authors: Abassi S, Emtiazi G, Hosseini-Abari A, Kim BG Tags: Curr Microbiol Source Type: research

Microbiota Associated with Dromedary Camel Milk from Algerian Sahara.
Abstract Despite the fact that camel milk represents a valuable food source, the fungal diversity of raw camel milk has been poorly studied so far. Here, we investigated the fungal and bacterial communities found in dromedary camel milk from Ghardaia, a representative region of Algerian Sahara. The application of both culture-dependent and independent molecular techniques, based on dHPLC analysis and metabarcoding of ITS region, provided a complementary biodiversity assessment of camel milk fungi which was composed of 15 different taxa. Yeast species belonged to Filobasidium, Naganishia, Malassezia, Mrakia, Rhodot...
Source: Current Microbiology - October 26, 2019 Category: Microbiology Authors: Amrouche T, Mounier J, Pawtowski A, Thomas F, Picot A Tags: Curr Microbiol Source Type: research

Clonal Dissemination of Clinical Isolates of Acinetobacter baumannii Carriers of 16S rRNA Methylase Genes in an Oncological Hospital in Recife, Brazil.
The objective was to characterize the susceptibility profile to aminoglycosides (amikacin and gentamicin) of clinical isolates of Acinetobacter spp. from an oncological hospital in Recife, and given the resistance to both antimicrobials, to characterize minimal inhibitory concentrations (MICs) of amikacin, gentamicin and tobramycin, the occurrence of 16S rRNA methylase genes (armA, rmtB, rmtC and rmtD) and of ß-lactamase gene (blaKPC) and the clonal profile. Isolates resistant to both antimicrobials, amikacin and gentamicin, were selected by disk diffusion technique in Mueller-Hinton agar and identified. Broth microd...
Source: Current Microbiology - October 26, 2019 Category: Microbiology Authors: da Paz Pereira JN, de Andrade CADN, da Costa Lima JL, de Lima Neto RG, de Araújo PSR, Maciel MAV Tags: Curr Microbiol Source Type: research

Degradation Mechanism of 4-Chlorobiphenyl by Consortium of  Pseudomonas sp. Strain CB-3 and Comamonas sp. Strain CD-2.
Degradation Mechanism of 4-Chlorobiphenyl by Consortium of Pseudomonas sp. Strain CB-3 and Comamonas sp. Strain CD-2. Curr Microbiol. 2019 Oct 24;: Authors: Xing Z, Hu T, Xiang Y, Qi P, Huang X Abstract Polychlorinated biphenyls (PCBs) are types of lasting environmental pollutants which are widely used in various industries. 4-chlorobiphenyl (4CBP) is a PCB which is harmful to the environment as well as humans. Two strains, CB-3 and CD-2, were isolated from the polluted soil of a chemical factory and could completely degrade 50 mg/L 4CBP within 12 h by co-culture. The consortium compris...
Source: Current Microbiology - October 24, 2019 Category: Microbiology Authors: Xing Z, Hu T, Xiang Y, Qi P, Huang X Tags: Curr Microbiol Source Type: research

Complete Genome Sequence of Sphingobacterium psychroaquaticum Strain SJ-25, an Aerobic Bacterium Capable of Suppressing Fungal Pathogens.
Abstract Using antagonistic bacterium is an effective method to control plant disease by fungal pathogens. An aerobic bacterium designated SJ-25, capable of suppressing Fusarium graminearum, Exserohilum turcicum, Pythium aphanidermatum, and Cochliobolus sativus, was isolated from farmland soil. The phylogenetic analysis revealed that strain SJ-25 belongs to the species of Sphingobacterium psychroaquaticum. The genome of strain SJ-25 consists of a 4,396,535-bp chromosome with a G+C content of 41.7 mol%; including 3696 CDS, 64 tRNA genes and six rRNA operons. Genomic analysis revealed that its genome contains multip...
Source: Current Microbiology - October 23, 2019 Category: Microbiology Authors: Xu L, Zhang H, Xing YT, Li N, Wang S, Sun JQ Tags: Curr Microbiol Source Type: research

Analysis of the Microbiota in the Fecal Material of Painted Turtles (Chrysemys picta).
Abstract We used high-throughput sequencing analysis, which targeted the hypervariable V3-V4 region of the bacterial 16S rRNA gene, to investigate the microbiota in fecal material from ten wild painted turtles (Chrysemys picta) captured in southeastern Wisconsin. The most predominant bacterial phylum detected in all samples was the Firmicutes (relative abundance for all samples 96.4% to 68.3%). The next most predominant phylum was Bacteroidetes (relative abundance for all samples 23.9% to 7.8%) in eight samples. Fusobacteria (relative abundance for all samples 22.2% to 0%) was the second most predominant in the ot...
Source: Current Microbiology - October 23, 2019 Category: Microbiology Authors: Fugate HM, Kapfer JM, McLaughlin RW Tags: Curr Microbiol Source Type: research

Glutathione is Involved in Detoxification of Peroxide and Root Nodule Symbiosis of Mesorhizobium huakuii.
Abstract Legumes interact with symbiotic rhizobia to produce nitrogen-fixation root nodules under nitrogen-limiting conditions. The contribution of glutathione (GSH) to this symbiosis and anti-oxidative damage was investigated using the M. huakuii gshB (encoding GSH synthetase) mutant. The gshB mutant grew poorly with different monosaccharides, including glucose, sucrose, fructose, maltose, or mannitol, as sole sources of carbon. The antioxidative capacity of gshB mutant was significantly decreased by these treatments with H2O2 under the lower concentrations and cumene hydroperoxide (CUOOH) under the higher concen...
Source: Current Microbiology - October 17, 2019 Category: Microbiology Authors: Luo S, Yin J, Peng Y, Xie J, Wu H, He D, Li X, Cheng G Tags: Curr Microbiol Source Type: research

Enterovibrio baiacu sp. nov.
We report here the novel species to encompass the isolate A649T (=CBAS 716T = CBRVS P1061T) obtained from viscera of the healthy pufferfish Sphoeroides spengleri (Family Tetraodontidae). Genomic taxonomy analysis demonstrates that the novel strain A649T had 
Source: Current Microbiology - October 16, 2019 Category: Microbiology Authors: Azevedo GPR, Mattsson HK, Appolinario LR, Calegario G, Leomil L, Walter JM, Campeão M, Tonon LAC, Moreira APB, Vidal L, Vieira VV, Otsuki K, Tschoeke DA, Swings J, Thompson FL, Thompson CC Tags: Curr Microbiol Source Type: research

Using the IPTG-Inducible Pgrac212 Promoter for Overexpression of Human Rhinovirus 3C Protease Fusions in the Cytoplasm of Bacillus subtilis Cells.
In conclusion, the combination of an inducible promoter Pgrac212 and a solubility tag allowed the overexpression of the HRV3C protease in the cytoplasm of B. subtilis. The resulting fusion protein was purified using a nickel column and was active in cleaving target proteins to remove the fusion tags. This study offers an effective method for producing recombinant proteins in the cytoplasm of endotoxin-free bacteria. PMID: 31612259 [PubMed - as supplied by publisher] (Source: Current Microbiology)
Source: Current Microbiology - October 14, 2019 Category: Microbiology Authors: Le VD, Phan TTP, Nguyen TM, Brunsveld L, Schumann W, Nguyen HD Tags: Curr Microbiol Source Type: research

Pontibacter beigongshangensis sp. nov., Isolated from the Mash of Wine.
Abstract A Gram-negative, aerobic, oval-shaped, and light red pigmented bacterium, designated T6-1T, was isolated from the mash of wine collected from a wine-making laboratory simulated fermenter located in Beijing, China. The optimal growth of T6-1T occurred at 30 °C, pH 7.0 with 1% NaCl. The sole respiratory quinone was menaquinone-7 (MK-7). The principal cellular fatty acids (>5%) were iso-C15:0, iso-C17:0 3OH, C16:1 ω5c, and iso-C17:0. The major polar lipids were PE (phosphatidylethanolamine), PL (unidentified phospholipid), and L1-2 (unidentified lipids). 16S rRNA phylogenetic analysis indic...
Source: Current Microbiology - October 10, 2019 Category: Microbiology Authors: Xu J, Sun L, Sun Z, Xing X, Li J, Lu X, Huang Y, Ren Q Tags: Curr Microbiol Source Type: research

In-Vitro Prebiotic Analysis of Microbiota Accessible Pectic Polysaccharides.
Abstract Pectin is a diverse polysaccharide comprising of polygalacturonic acid, rhamnogalactouronan, and neutral polysaccharides (arabinan and arabinogalactan) as side chains or branches. They are resistant to salivary amylase and gastric juice. In the present study the prebiotic potentials of different pectic derived substrates were performed by using intestinal cultures and measuring the growth, change in pH and short chain fatty acid, (SCFA) production. Arabinan, arabinogalactan, and rhamnogalacturonan were fermented by probiotic bacteria such as Bifidobacterium breve, Bi. longum, Bi. bifidum, and Bacteroides ...
Source: Current Microbiology - October 4, 2019 Category: Microbiology Authors: Tingirikari JMR Tags: Curr Microbiol Source Type: research

Identification and Characterization of Cronobacter Strains Isolated from Environmental Samples.
Abstract As an emerging food-borne pathogen, Cronobacter species are ubiquitous in the food and environment. In order to know the characteristics of Cronobacter spp. from the environment, we isolated Cronobacter spp. from soil and water, and then studied the molecular typing and antibiotic resistance characteristics of these isolates. In 2016, 141 soil and water samples were collected from farms and Riverside Park in Beijing. Isolates were identified by real-time PCR, 16s rRNA sequencing, and whole-genome sequencing. Molecular subtyping of these isolates was characterized by pulsed-field gel electrophoresis, multi...
Source: Current Microbiology - October 4, 2019 Category: Microbiology Authors: Hu J, Li X, Du X, Cui Z, Cui J Tags: Curr Microbiol Source Type: research

Enhanced Production of Shigella flexneri Polysaccharide from a Newly Devised Silicate Method as a Potential Vaccine Conjugate.
Abstract Conventional glycoconjugates are prepared with polysaccharides (PS) isolated from bacterial sources by fermentation technique. This approach has some major challenges like lower yield of PS, impurities and usage of hazardous chemicals. Reports on efficient and enhanced production of PS from Shigella flexneri is meager in literature. Hence, in the current study, three different types of media namely Yeast extract medium, Shigella sonnei-defined medium and synthetic medium were utilized for the culture of S. flexneri. Among the selected media it was recognized that the culture of S. flexneri harvested in sy...
Source: Current Microbiology - October 3, 2019 Category: Microbiology Authors: Narayana PVSLSS, Ray Dutta J Tags: Curr Microbiol Source Type: research

Streptococcus koreensis sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion.
Abstract A novel facultative anaerobic, Gram-stain-positive coccus, strain JS71T, was isolated from the human subgingival dental plaque of a periodontitis lesion. Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) revealed that the strain belonged to the genus Streptococcus. The 16S rDNA sequence had high similarity with Streptococcus rubneri DSM 26920T (98.6%), Streptococcus parasanguinis ATCC 15912T (98.5%), and Streptococcus australis CCUG 45919T (98.3%). The genome of strain JS71T was 2,009,592 bp in length. The DNA G+C content of the strain was 42.1 mol%. Average nucleotide identit...
Source: Current Microbiology - September 30, 2019 Category: Microbiology Authors: Lim YK, Park SN, Shin JH, Ji S, Jo E, Chang YH, Shin Y, Paek J, Kim H, Kook JK Tags: Curr Microbiol Source Type: research