Contrasting Composition, Diversity and Predictive Metabolic Potential of the Rhizobacterial Microbiomes Associated with Native and Invasive Prosopis Congeners
In conclusion, our results demonstrate that the co-occurring native and invasive Prosopis species have significantly different rhizosphere bacterial communities in terms of composition, diversity and their predictive metabolic potentials. In addition, the rhizosphere microbiome of invasive Prosopis proffers it a fitness advantage and influences invasion success of the species.PMID:33837467 | DOI:10.1007/s00284-021-02473-1 (Source: Current Microbiology)
Source: Current Microbiology - April 10, 2021 Category: Microbiology Authors: Rishabh Kaushik Maharaj K Pandit Laura A Meyerson Diptaraj S Chaudhari Meesha Sharma Dhiraj Dhotre Yogesh S Shouche Source Type: research

Antibacterial and Antifungal Activities and Toxicity of the Essential Oil from Callistemon viminalis Complexed with β-Cyclodextrin
Curr Microbiol. 2021 Apr 10. doi: 10.1007/s00284-021-02480-2. Online ahead of print.ABSTRACTThe essential oil from Callistemon viminalis (EOC) is rich in monoterpenes, with a variety of biological properties: antibacterial, antifungal, insecticide, and antioxidant. Inclusion complexes (ICs) with cyclodextrins (CDs) is an alternative to prevent toxicity, improve the activity, and reduce the concentration to be used. Thus, the objective of this work was to prepare an IC (EOC/β-CD) and evaluate the antibacterial, antifungal and phospholipase activities, as well as the toxicity. Antimicrobial activity used the agar diffus...
Source: Current Microbiology - April 10, 2021 Category: Microbiology Authors: Lucimara N S B Martins Adneia F A Venceslau Rafaela M Brand ão Mariana A Braga Luis Roberto Batista Maria das Gra ças Cardoso Luciana M A Pinto Source Type: research

Phenotypic Identification and Genotypic Characterization of Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Iran
In conclusion, our study provided valuable information regarding the plasmid-mediated AmpC β-lactamase gene content, antibiotic resistance, and confirmatory phenotypic tests for AmpC β-lactamases in E. coli and K. pneumoniae isolates from clinical sources.PMID:33837818 | DOI:10.1007/s00284-021-02479-9 (Source: Current Microbiology)
Source: Current Microbiology - April 10, 2021 Category: Microbiology Authors: Saeedeh Robatjazi Farhad Nikkhahi Mojtaba Niazadeh Seyed Mahmoud Amin Marashi Amir Peymani Amir Javadi Amir Hossein Kashani Source Type: research

Endophytic Bacteria from the Sahara Desert Protect Tomato Plants Against Botrytis cinerea Under Different Experimental Conditions
In this study, different bacterial endophytic strains were isolated from different plants primed to live in an arid area, namely, the Sahara Desert. Up to 27 of these strains were selected based on their ability to inhibit Botrytis cinerea growth in dual-culture assay and by bacterial volatiles. The results presented in this study show the capacity of most of the bacterial strains to protect Solanum lycopersicum against the pathogenic fungus B. cinerea, under different experimental conditions. Five of these strains induced susceptibility in tomato plants and no callose accumulation upon fungal infection, pointing to callos...
Source: Current Microbiology - April 9, 2021 Category: Microbiology Authors: Nadira Oukala Julia Pastor-Fern ández Neus Sanmart ín Kamel Aissat Victoria Pastor Source Type: research

The Occurrence of Triple Catalytic Characteristics of Yeast Lipases and Their Application Prospects in Biodiesel Production from Non-Edible Jatropha curcas Oil in a Solvent-Free System
Curr Microbiol. 2021 Apr 9. doi: 10.1007/s00284-021-02448-2. Online ahead of print.ABSTRACTExtracellular and cell-bound lipase-producing yeasts were isolated from the palm oil mill wastes and investigated for their potential uses as biocatalysts in biodiesel production. Twenty-six yeast strains were qualitatively screened as lipase producers. From those yeast strains, only six were selected and screened further for quantitative lipase production.The phylogenetic affiliations of the yeast strains were confirmed by investigating the D1/D2 domains of 26S rDNA and ITS1-5.8S-ITS2 molecular regions of the six yeast strains selec...
Source: Current Microbiology - April 9, 2021 Category: Microbiology Authors: Khurshid Ahmed Baloch Apichat Upaichit Benjamas Cheirsilp Fidia Fibriana Source Type: research

Complete Genome Sequence of a Novel Bacteriophage RpY1 Infecting Ralstonia solanacearum Strains
In this study, we isolated 72 phages from the various crop cultivated soils in Korea using five different strains of R. solanacearum. Among 72 phages, phage RpY1 was selected for further study based on the specificity of the targeted host. This phage was identified as a member of Podoviridae with a head measuring 60-70 nm in length and short tail according to the morphology of transmission electron microscopy images. The genome size of phage RpY1 is 43,284 bp with G + C content of 61.4% and 53 open reading frames (ORFs), including 18 annotated ORFs and 35 hypothetical proteins. This phage genome showed no homology to the g...
Source: Current Microbiology - April 9, 2021 Category: Microbiology Authors: Seung Yeup Lee Roniya Thapa Magar Hyo Jeong Kim Kihyuck Choi Seon-Woo Lee Source Type: research

Endophytic Bacteria from the Sahara Desert Protect Tomato Plants Against Botrytis cinerea Under Different Experimental Conditions
In this study, different bacterial endophytic strains were isolated from different plants primed to live in an arid area, namely, the Sahara Desert. Up to 27 of these strains were selected based on their ability to inhibit Botrytis cinerea growth in dual-culture assay and by bacterial volatiles. The results presented in this study show the capacity of most of the bacterial strains to protect Solanum lycopersicum against the pathogenic fungus B. cinerea, under different experimental conditions. Five of these strains induced susceptibility in tomato plants and no callose accumulation upon fungal infection, pointing to callos...
Source: Current Microbiology - April 9, 2021 Category: Microbiology Authors: Nadira Oukala Julia Pastor-Fern ández Neus Sanmart ín Kamel Aissat Victoria Pastor Source Type: research

The Occurrence of Triple Catalytic Characteristics of Yeast Lipases and Their Application Prospects in Biodiesel Production from Non-Edible Jatropha curcas Oil in a Solvent-Free System
Curr Microbiol. 2021 Apr 9. doi: 10.1007/s00284-021-02448-2. Online ahead of print.ABSTRACTExtracellular and cell-bound lipase-producing yeasts were isolated from the palm oil mill wastes and investigated for their potential uses as biocatalysts in biodiesel production. Twenty-six yeast strains were qualitatively screened as lipase producers. From those yeast strains, only six were selected and screened further for quantitative lipase production.The phylogenetic affiliations of the yeast strains were confirmed by investigating the D1/D2 domains of 26S rDNA and ITS1-5.8S-ITS2 molecular regions of the six yeast strains selec...
Source: Current Microbiology - April 9, 2021 Category: Microbiology Authors: Khurshid Ahmed Baloch Apichat Upaichit Benjamas Cheirsilp Fidia Fibriana Source Type: research

Complete Genome Sequence of a Novel Bacteriophage RpY1 Infecting Ralstonia solanacearum Strains
In this study, we isolated 72 phages from the various crop cultivated soils in Korea using five different strains of R. solanacearum. Among 72 phages, phage RpY1 was selected for further study based on the specificity of the targeted host. This phage was identified as a member of Podoviridae with a head measuring 60-70 nm in length and short tail according to the morphology of transmission electron microscopy images. The genome size of phage RpY1 is 43,284 bp with G + C content of 61.4% and 53 open reading frames (ORFs), including 18 annotated ORFs and 35 hypothetical proteins. This phage genome showed no homology to the g...
Source: Current Microbiology - April 9, 2021 Category: Microbiology Authors: Seung Yeup Lee Roniya Thapa Magar Hyo Jeong Kim Kihyuck Choi Seon-Woo Lee Source Type: research

Molecular Identification of Penicillium sp. Isolated from Citrus Fruits
This study provided a useful molecular approach to identify different Penicillium species by sequencing ITS region, focus on the pathogenicity, compare between P. expansum isolates and their ability in patulin production.PMID:33829281 | DOI:10.1007/s00284-021-02463-3 (Source: Current Microbiology)
Source: Current Microbiology - April 8, 2021 Category: Microbiology Authors: Eman Gamal Abd Elnaser Mohamed El-Dawy Youssuf Ahmed Gherbawy Sabry Hassan Mohamed Ahmed Hussein Source Type: research

Characterization and Pathogenicity of Rhizoctonia Species Causing Root Rot and Damping-off on Sugar Beet in Turkey
Curr Microbiol. 2021 Apr 7. doi: 10.1007/s00284-021-02470-4. Online ahead of print.ABSTRACTDuring 2015-2017 growing seasons, seventy-one isolates of Rhizoctonia spp. were obtained from the samples collected from the sugar beet fields of Konya province, which is one of the most important sugar beet growing areas of Turkey. Anastomosis groups (AGs) of Rhizoctonia spp. isolates were determined by hyphal conjugation with the tester strains and sequence analysis of the rDNA ITS region. The obtained data confirmed the species identity of 61 isolates out of 71 as R. solani (AG-2-2-IIIB, AG-4-(HGI, HGII, HGIII), AG-5, AG-11) and t...
Source: Current Microbiology - April 8, 2021 Category: Microbiology Authors: Meltem Avan G ülsüm Palacıoğlu Cemre Aksoy R ıza Kaya Harun Bayraktar Yakup Zekai Kat ırcıoğlu Salih Maden Source Type: research

Isolation and Identification of Pseudomonas sp. Strain DY-1 from Agricultural Soil and Its Degradation Effect on Prometryne
This study presents a bacterial strain isolated from soil samples with a prometryne application history, designated strain DY-1. Strain DY-1, identified as Pseudomonas sp., is capable of utilizing prometryne as a sole carbon source for growth and degrading 100% of prometryne within 48 h from an initial concentration of 50 mg L-1. To further optimize the degradation of prometryne, the prometryne concentration, temperature, pH, and salt concentration were examined. The optimal conditions for degradation of prometryne by strain DY-1 were an initial prometryne concentration of 50 mg L-1, 30 °C, pH 7-8, and NaCl concentrati...
Source: Current Microbiology - April 8, 2021 Category: Microbiology Authors: Dong Liang Ming-Yue Ding Chang-Yixin Xiao Ya-Wen Shen Yin-Yue Wang Hai-Tao Li Rong-Mei Liu Ji-Guo Gao Source Type: research

Accumulation of Ectoines By Halophilic Bacteria Isolated from Fermented Shrimp Paste: An Adaptation Mechanism to Salinity, Temperature, and pH Stress
This study found that most halophilic bacteria isolated from shrimp paste accumulated ectoines (ectoine and hydroxyectoine) as protective osmotic agents. Five isolated bacteria, including three high ectoine producers and two high hydroxyectoine producers, were selected for further study. Based on their morphological and biochemical characteristics and 16S rRNA gene sequences, the five strains were classified into three genera: Salinivibrio (strains M7 and M316), Salimicrobium (strains M31 and M69), and Vibrio (strain M92). The accumulation of ectoines by Salimicrobium species is reported here for the first time. The effect...
Source: Current Microbiology - April 8, 2021 Category: Microbiology Authors: Doan Van Thuoc Tran Thi Loan Nguyen Thi Tra Source Type: research

Prevalence of Campylobacter spp. in Chicken Carcasses in Slaughterhouses from South of Brazil
Curr Microbiol. 2021 Apr 8. doi: 10.1007/s00284-021-02478-w. Online ahead of print.ABSTRACTCampylobacteriosis has become common cause of diarrhea in humans and is associated with Guillain-Barré Syndrome, Reactive Arthritis and Irritable Bowel Syndrome is caused mainly by contaminated food and water intake in which the majority occurs from manipulation, preparation and consumption of poultry meat. The aim of this study was to estimate the prevalence of Campylobacter in chicken carcasses from slaughterhouses located in the states of Parana, Santa Catarina and Rio Grande do Sul in the South of Brazil. The samples were ...
Source: Current Microbiology - April 8, 2021 Category: Microbiology Authors: Carla Susana Rodrigues Paulo Marcel Armendaris Claudia Val éria Gonçalves Cordeiro de Sá Jo ão Paulo Amaral Haddad Cristiano Barros de Melo Source Type: research

Molecular Identification of Penicillium sp. Isolated from Citrus Fruits
This study provided a useful molecular approach to identify different Penicillium species by sequencing ITS region, focus on the pathogenicity, compare between P. expansum isolates and their ability in patulin production.PMID:33829281 | DOI:10.1007/s00284-021-02463-3 (Source: Current Microbiology)
Source: Current Microbiology - April 8, 2021 Category: Microbiology Authors: Eman Gamal Abd Elnaser Mohamed El-Dawy Youssuf Ahmed Gherbawy Sabry Hassan Mohamed Ahmed Hussein Source Type: research

Characterization and Pathogenicity of Rhizoctonia Species Causing Root Rot and Damping-off on Sugar Beet in Turkey
Curr Microbiol. 2021 Apr 7. doi: 10.1007/s00284-021-02470-4. Online ahead of print.ABSTRACTDuring 2015-2017 growing seasons, seventy-one isolates of Rhizoctonia spp. were obtained from the samples collected from the sugar beet fields of Konya province, which is one of the most important sugar beet growing areas of Turkey. Anastomosis groups (AGs) of Rhizoctonia spp. isolates were determined by hyphal conjugation with the tester strains and sequence analysis of the rDNA ITS region. The obtained data confirmed the species identity of 61 isolates out of 71 as R. solani (AG-2-2-IIIB, AG-4-(HGI, HGII, HGIII), AG-5, AG-11) and t...
Source: Current Microbiology - April 8, 2021 Category: Microbiology Authors: Meltem Avan G ülsüm Palacıoğlu Cemre Aksoy R ıza Kaya Harun Bayraktar Yakup Zekai Kat ırcıoğlu Salih Maden Source Type: research

Isolation Chip Increases Culturable Bacterial Diversity and Reduces Cultivation Bias
In this study, we evaluated the ability of isolation chip (ichip) to reduce cultivation bias. We used the ichip and petri dish plating methods to cultivate bacteria from soil contaminated with (contaminated soil) or without (natural soil) crude oil. Ichip improved the richness and evenness of bacterial isolates in both the natural and contaminated soil samples. Using the petri dish plating method, Pseudomonas and Lysinibacillus isolates were found to be the most abundant, with over 50% of the relative abundance in the natural and oil-polluted soil-cultured communities, respectively. In comparison, using the ichip method, t...
Source: Current Microbiology - April 6, 2021 Category: Microbiology Authors: Xinwu Liu Miaoxiao Wang Yong Nie Xiao-Lei Wu Source Type: research

Isolation Chip Increases Culturable Bacterial Diversity and Reduces Cultivation Bias
In this study, we evaluated the ability of isolation chip (ichip) to reduce cultivation bias. We used the ichip and petri dish plating methods to cultivate bacteria from soil contaminated with (contaminated soil) or without (natural soil) crude oil. Ichip improved the richness and evenness of bacterial isolates in both the natural and contaminated soil samples. Using the petri dish plating method, Pseudomonas and Lysinibacillus isolates were found to be the most abundant, with over 50% of the relative abundance in the natural and oil-polluted soil-cultured communities, respectively. In comparison, using the ichip method, t...
Source: Current Microbiology - April 6, 2021 Category: Microbiology Authors: Xinwu Liu Miaoxiao Wang Yong Nie Xiao-Lei Wu Source Type: research

Genome-Wide Identification and Analysis of Chromosomally Integrated Putative Prophages Associated with Clinical Klebsiella pneumoniae Strains
In this study, 254 strains of K. pneumoniae were screened for the presence of prophages using the PHASTER tool. Very few strains lacked prophages (3.1%), while the remaining harboured both intact (811) and defective prophages (709). A subset of 42 unique strains of K. pneumoniae was chosen for further analysis. Our analysis revealed the presence of 110 complete prophages which were further classified as belonging to Myoviridae (67.3%), Siphoviridae (28.2%) and Podoviridae family (4.5%). An alignment of the 110 complete, prophage genome sequences clustered the prophages into 16 groups and 3 singletons. While none of the pro...
Source: Current Microbiology - April 4, 2021 Category: Microbiology Authors: Pallavi Baliga Malathi Shekar Girisha Shivani Kallappa Source Type: research

Moheibacter lacus sp. nov., Isolated from Freshwater Lake Sediment
Curr Microbiol. 2021 Apr 3. doi: 10.1007/s00284-021-02465-1. Online ahead of print.ABSTRACTA Gram-stain-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-motile bacterium, designated BDHS18T, was isolated from the sediment of the Hasuhai Lake, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this strain belongs to the genus Moheibacter in the family Flavobacteriaceae and its closest relative was Moheibacter sediminis JCM 19634T (96.0%), followed by Moheibacter stercoris DSM 29388T (95.3%). Cells of strain BDHS18T were catalase-positive and oxidase-negative. Strain BDHS18T was found to...
Source: Current Microbiology - April 4, 2021 Category: Microbiology Authors: Yuan Liu Ya-Qi Chang Chao-Nan Wang Meng-Qi Ye Ming-Yi Wang Zong-Jun Du Source Type: research

Genome-Wide Identification and Analysis of Chromosomally Integrated Putative Prophages Associated with Clinical Klebsiella pneumoniae Strains
In this study, 254 strains of K. pneumoniae were screened for the presence of prophages using the PHASTER tool. Very few strains lacked prophages (3.1%), while the remaining harboured both intact (811) and defective prophages (709). A subset of 42 unique strains of K. pneumoniae was chosen for further analysis. Our analysis revealed the presence of 110 complete prophages which were further classified as belonging to Myoviridae (67.3%), Siphoviridae (28.2%) and Podoviridae family (4.5%). An alignment of the 110 complete, prophage genome sequences clustered the prophages into 16 groups and 3 singletons. While none of the pro...
Source: Current Microbiology - April 4, 2021 Category: Microbiology Authors: Pallavi Baliga Malathi Shekar Girisha Shivani Kallappa Source Type: research

Moheibacter lacus sp. nov., Isolated from Freshwater Lake Sediment
Curr Microbiol. 2021 Apr 3. doi: 10.1007/s00284-021-02465-1. Online ahead of print.ABSTRACTA Gram-stain-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-motile bacterium, designated BDHS18T, was isolated from the sediment of the Hasuhai Lake, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this strain belongs to the genus Moheibacter in the family Flavobacteriaceae and its closest relative was Moheibacter sediminis JCM 19634T (96.0%), followed by Moheibacter stercoris DSM 29388T (95.3%). Cells of strain BDHS18T were catalase-positive and oxidase-negative. Strain BDHS18T was found to...
Source: Current Microbiology - April 4, 2021 Category: Microbiology Authors: Yuan Liu Ya-Qi Chang Chao-Nan Wang Meng-Qi Ye Ming-Yi Wang Zong-Jun Du Source Type: research

Marinisubtilis pacificus gen. nov., sp. nov., a Member of the Family Microbacteriaceae Isolated From a Deep-Sea Seamount
Curr Microbiol. 2021 Apr 2. doi: 10.1007/s00284-021-02468-y. Online ahead of print.ABSTRACTA Gram-stain-positive, yellow, aerobic, slender rod-shaped bacterial strain, designated KN1116T, was isolated from a deep-sea seamount. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain KN1116T was related to the genus Chryseoglobus and had highest 16S rRNA gene sequence identity with Chryseoglobus frigidaquae CW1T (98.5%). The predominant cellular fatty acids were anteiso-C15:0 and iso-C16:0. The quinone system for strain KN1116T comprised menaquinone MK-12, MK-11, MK-10 and MK-13. The polar lipid profile cont...
Source: Current Microbiology - April 3, 2021 Category: Microbiology Authors: Fang Qin Wen-Xuan He De-Chao Zhang Source Type: research

Inhibition of Phospholipase by Orlistat as an Alternate Therapy to Combat Opportunistic Mycosis Caused by C. albicans
Curr Microbiol. 2021 Apr 3. doi: 10.1007/s00284-021-02476-y. Online ahead of print.ABSTRACTCandida albicans is one of the most important etiological agents causing an opportunistic mycosis, candidiasis. In the past, it was perceived to be associated with immunocompromised patients only. However, it has now been reported with several clinical complications with varying severity. Additionally, increasing incidences of multiple drug resistance associated with the infections have complicated its treatment as well. Therefore, an investigation of alternate therapy, for instance, inhibition of the virulence factors is desperately...
Source: Current Microbiology - April 3, 2021 Category: Microbiology Authors: Hardi Sinde Priyanka Patel Kunjan M Kikani Dhyey R Kothari Bhavtosh A Kikani Source Type: research

Rhamnolipids Application for the Removal of Vanadium from Contaminated Sediment
Curr Microbiol. 2021 Apr 3. doi: 10.1007/s00284-021-02445-5. Online ahead of print.ABSTRACTThe use of biosurfactants in bioremediation of hydrocarbons and in the removal of heavy metals in crude oils is considered an attractive subject. The vanadium pollution in soil and sediments had attracted research interest in exploring eco-friendly methods of remediation. The present study was conducted to evaluate the potential of a biosurfactant to remove vanadium from artificially contaminated sand. The biosurfactant producer's strain selection process was carried out from 23 strains in two steps. In the primary screening, four pr...
Source: Current Microbiology - April 3, 2021 Category: Microbiology Authors: Yaima Barrios San Mart ín Heidy F Toledo Le ón Arelis Ábalos Rodríguez Ana M Marqu és Maria I S ánchez López Source Type: research

Marinisubtilis pacificus gen. nov., sp. nov., a Member of the Family Microbacteriaceae Isolated From a Deep-Sea Seamount
Curr Microbiol. 2021 Apr 2. doi: 10.1007/s00284-021-02468-y. Online ahead of print.ABSTRACTA Gram-stain-positive, yellow, aerobic, slender rod-shaped bacterial strain, designated KN1116T, was isolated from a deep-sea seamount. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain KN1116T was related to the genus Chryseoglobus and had highest 16S rRNA gene sequence identity with Chryseoglobus frigidaquae CW1T (98.5%). The predominant cellular fatty acids were anteiso-C15:0 and iso-C16:0. The quinone system for strain KN1116T comprised menaquinone MK-12, MK-11, MK-10 and MK-13. The polar lipid profile cont...
Source: Current Microbiology - April 3, 2021 Category: Microbiology Authors: Fang Qin Wen-Xuan He De-Chao Zhang Source Type: research

Inhibition of Phospholipase by Orlistat as an Alternate Therapy to Combat Opportunistic Mycosis Caused by C. albicans
Curr Microbiol. 2021 Apr 3. doi: 10.1007/s00284-021-02476-y. Online ahead of print.ABSTRACTCandida albicans is one of the most important etiological agents causing an opportunistic mycosis, candidiasis. In the past, it was perceived to be associated with immunocompromised patients only. However, it has now been reported with several clinical complications with varying severity. Additionally, increasing incidences of multiple drug resistance associated with the infections have complicated its treatment as well. Therefore, an investigation of alternate therapy, for instance, inhibition of the virulence factors is desperately...
Source: Current Microbiology - April 3, 2021 Category: Microbiology Authors: Hardi Sinde Priyanka Patel Kunjan M Kikani Dhyey R Kothari Bhavtosh A Kikani Source Type: research

Rhamnolipids Application for the Removal of Vanadium from Contaminated Sediment
Curr Microbiol. 2021 Apr 3. doi: 10.1007/s00284-021-02445-5. Online ahead of print.ABSTRACTThe use of biosurfactants in bioremediation of hydrocarbons and in the removal of heavy metals in crude oils is considered an attractive subject. The vanadium pollution in soil and sediments had attracted research interest in exploring eco-friendly methods of remediation. The present study was conducted to evaluate the potential of a biosurfactant to remove vanadium from artificially contaminated sand. The biosurfactant producer's strain selection process was carried out from 23 strains in two steps. In the primary screening, four pr...
Source: Current Microbiology - April 3, 2021 Category: Microbiology Authors: Yaima Barrios San Mart ín Heidy F Toledo Le ón Arelis Ábalos Rodríguez Ana M Marqu és Maria I S ánchez López Source Type: research

Glyphosate Biodegradation Potential in Soil Based on Glycine Oxidase Gene (thiO) from Bradyrhizobium
Curr Microbiol. 2021 Apr 2. doi: 10.1007/s00284-021-02467-z. Online ahead of print.ABSTRACTDespite the intensive use of glyphosate (GP) and its ubiquitous presence in the environment, studies addressing the presence of microbial genes involved in glyphosate degradation in natural conditions are scarce. Based on the agronomical importance of Bradyrhizobium genus and its metabolic versatility, we tested the hypothesis that species or genotypes of Bradyrhizobium could be a proxy for GP degrader potential in soil. A quantitative PCR assay was designed to target a specific region of the glycine oxidase gene (thiO), involved in ...
Source: Current Microbiology - April 2, 2021 Category: Microbiology Authors: Keren Hern ández Guijarro Eduardo De Ger ónimo Leonardo Erijman Source Type: research

Glyphosate Biodegradation Potential in Soil Based on Glycine Oxidase Gene (thiO) from Bradyrhizobium
Curr Microbiol. 2021 Apr 2. doi: 10.1007/s00284-021-02467-z. Online ahead of print.ABSTRACTDespite the intensive use of glyphosate (GP) and its ubiquitous presence in the environment, studies addressing the presence of microbial genes involved in glyphosate degradation in natural conditions are scarce. Based on the agronomical importance of Bradyrhizobium genus and its metabolic versatility, we tested the hypothesis that species or genotypes of Bradyrhizobium could be a proxy for GP degrader potential in soil. A quantitative PCR assay was designed to target a specific region of the glycine oxidase gene (thiO), involved in ...
Source: Current Microbiology - April 2, 2021 Category: Microbiology Authors: Keren Hern ández Guijarro Eduardo De Ger ónimo Leonardo Erijman Source Type: research

Ruegeria haliotis sp. nov., Isolated from the Gut of the Abalone Haliotis rubra
Curr Microbiol. 2021 Apr 1. doi: 10.1007/s00284-021-02450-8. Online ahead of print.ABSTRACTA Gram-stain-negative, aerobic, non-motile, white-pigmented, short rod-shaped, and alginate-degrading bacterium, designated B1Z28T, was isolated from the gut of the abalone Haliotis rubra obtained at Weihai, China. Strain B1Z28T was found to grow at 4-35 °C, pH 6.5-9.0, and in the presence of 0.5-8.0% (w/v) NaCl. Cells were positive for oxidase and catalase activity. The 16S rRNA-based phylogenetic analysis revealed that the nearest phylogenetic neighbors of strain B1Z28T were Tritonibacter scottomollicae MCCC 1A06440T (98.1%), R...
Source: Current Microbiology - April 1, 2021 Category: Microbiology Authors: Wen-Rui Cao Dan-Dan Shang Bang-Tao Liu Yi-Hao Hu Xun-Ke Sun Yuan-Yuan Sun Ming-Yu Jiang Zong-Jun Du Source Type: research

Compost Samples from Different Temperature Zones as a Model to Study Co-occurrence of Thermophilic and Psychrophilic Bacterial Population: a Metagenomics Approach
In this study, using a metagenomic approach, we explore the bacterial diversity of compost sites categorized based on their ambient temperatures. The two sites were Reckong Peo in the lower Himalayas and Tambaram in the southern region of the country, namely, CPR and CT. Following assembly of the raw reads from shotgun metagenomics, similarity hits were generated using NCBI BLAST + and SILVA database. A total of 1463 and 1483 species were annotated from CPR and CT. A species-level annotation was performed using a python-based literature search pipeline revealing their growth characteristics. Thermophiles Thermomonospora cu...
Source: Current Microbiology - March 31, 2021 Category: Microbiology Authors: Jithin S Sunny Anuradha Natarajan Khairun Nisha Lilly M Saleena Source Type: research

Bioaugmentation of Soil with Pseudomonas monteilii Strain Eliminates Inhibition of Okra (Abelmoschus esculentus) Seed Germination by m-Cresol
Curr Microbiol. 2021 Mar 31. doi: 10.1007/s00284-021-02438-4. Online ahead of print.ABSTRACTCresols are ubiquitous in nature due to their bulk production and end uses in various industrial processes as well as due to their natural presence. They are highly toxic to both fauna and flora and are included in the list of priority pollutants. In the present study, the effect of m-cresol on germination of ten different crop seeds was tested and the seeds of okra and eggplant were found to be very sensitive, okra being the most vulnerable. Okra seeds lost its viability in the presence of m-cresol, which was proportionate to its c...
Source: Current Microbiology - March 31, 2021 Category: Microbiology Authors: M Ajisha T C Shaima Soumya V Menon A A Mohammad Kunhi Source Type: research

Green Formulation of Microbial Biopolyesteric Nanocarriers Toward In Vitro Drug Delivery and Its Characterization
Curr Microbiol. 2021 Mar 31. doi: 10.1007/s00284-021-02464-2. Online ahead of print.ABSTRACTIn the present study, formulation and characterization of microbial biopolyesteric nanocarrier (MBPNc) was reported for in vitro controlled release of the drugs, viz., amoxicillin and levofloxacin. The synthesis of microbial biopolyester, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticle was done by a triple emulsion method and loaded with amoxicillin and levofloxacin to improve its curative bioavailability. The synthesized MBPNc was found to be spherical in shape with a size range of 50-100 nm which was confirmed through Tr...
Source: Current Microbiology - March 31, 2021 Category: Microbiology Authors: Nupur Ojha Nilanjana Das Source Type: research

Compost Samples from Different Temperature Zones as a Model to Study Co-occurrence of Thermophilic and Psychrophilic Bacterial Population: a Metagenomics Approach
In this study, using a metagenomic approach, we explore the bacterial diversity of compost sites categorized based on their ambient temperatures. The two sites were Reckong Peo in the lower Himalayas and Tambaram in the southern region of the country, namely, CPR and CT. Following assembly of the raw reads from shotgun metagenomics, similarity hits were generated using NCBI BLAST + and SILVA database. A total of 1463 and 1483 species were annotated from CPR and CT. A species-level annotation was performed using a python-based literature search pipeline revealing their growth characteristics. Thermophiles Thermomonospora cu...
Source: Current Microbiology - March 31, 2021 Category: Microbiology Authors: Jithin S Sunny Anuradha Natarajan Khairun Nisha Lilly M Saleena Source Type: research

Bioaugmentation of Soil with Pseudomonas monteilii Strain Eliminates Inhibition of Okra (Abelmoschus esculentus) Seed Germination by m-Cresol
Curr Microbiol. 2021 Mar 31. doi: 10.1007/s00284-021-02438-4. Online ahead of print.ABSTRACTCresols are ubiquitous in nature due to their bulk production and end uses in various industrial processes as well as due to their natural presence. They are highly toxic to both fauna and flora and are included in the list of priority pollutants. In the present study, the effect of m-cresol on germination of ten different crop seeds was tested and the seeds of okra and eggplant were found to be very sensitive, okra being the most vulnerable. Okra seeds lost its viability in the presence of m-cresol, which was proportionate to its c...
Source: Current Microbiology - March 31, 2021 Category: Microbiology Authors: M Ajisha T C Shaima Soumya V Menon A A Mohammad Kunhi Source Type: research

Green Formulation of Microbial Biopolyesteric Nanocarriers Toward In Vitro Drug Delivery and Its Characterization
Curr Microbiol. 2021 Mar 31. doi: 10.1007/s00284-021-02464-2. Online ahead of print.ABSTRACTIn the present study, formulation and characterization of microbial biopolyesteric nanocarrier (MBPNc) was reported for in vitro controlled release of the drugs, viz., amoxicillin and levofloxacin. The synthesis of microbial biopolyester, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticle was done by a triple emulsion method and loaded with amoxicillin and levofloxacin to improve its curative bioavailability. The synthesized MBPNc was found to be spherical in shape with a size range of 50-100 nm which was confirmed through Tr...
Source: Current Microbiology - March 31, 2021 Category: Microbiology Authors: Nupur Ojha Nilanjana Das Source Type: research

Evaluation of Antimicrobial and Antioxidant Potential of Essential Oil from Croton piauhiensis M üll. Arg
This study indicated that the EOCp may be a potential agent against infections caused by bacterial biofilms, and act as a protective agent against ROS and oxidative stress.PMID:33782740 | DOI:10.1007/s00284-021-02449-1 (Source: Current Microbiology)
Source: Current Microbiology - March 30, 2021 Category: Microbiology Authors: Jean Parcelli Costa do Vale Mayron Alves Vasconcelos Francisco Vassiliepe Sousa Arruda Nairley Cardoso S á Firmino Anna Lu ísa Pereira Alexandre Lopes Andrade Silvana Saker-Sampaio Alexandre Holanda Sampaio Emmanuel Silva Marinho Alexandre Magno Rodrigu Source Type: research

Evaluation of Antimicrobial and Antioxidant Potential of Essential Oil from Croton piauhiensis M üll. Arg
This study indicated that the EOCp may be a potential agent against infections caused by bacterial biofilms, and act as a protective agent against ROS and oxidative stress.PMID:33782740 | DOI:10.1007/s00284-021-02449-1 (Source: Current Microbiology)
Source: Current Microbiology - March 30, 2021 Category: Microbiology Authors: Jean Parcelli Costa do Vale Mayron Alves Vasconcelos Francisco Vassiliepe Sousa Arruda Nairley Cardoso S á Firmino Anna Lu ísa Pereira Alexandre Lopes Andrade Silvana Saker-Sampaio Alexandre Holanda Sampaio Emmanuel Silva Marinho Alexandre Magno Rodrigu Source Type: research

Production of Alkaline Proteases using Aspergillus sp. Isolated from Injera: RSM-GA Based Process Optimization and Enzyme Kinetics Aspect
This study focused on isolating the fungus producing proteases from, a typical Ethiopian food, Injera. Further, the process optimization for protease production using response surface methodology (RSM) and the characterization of the acquired protease were investigated. The 18S rDNA gene sequence homology of the fungus isolate revealed that it was Aspergillus sp. Further, it was deposited in NCBI GenBank with accession number MK4262821. Using the isolate, owing to maximize the protease production, the independent process parameters, temperature, pH, and sucrose concentration were optimized using RSM followed by a genetic a...
Source: Current Microbiology - March 29, 2021 Category: Microbiology Authors: Surafel Mustefa Beyan S Venkatesa Prabhu Tsegazeab K Mumecha Mesfin T Gemeda Source Type: research

Isolation and Identification of Cronobacter spp. from Fish and Shellfish Sold in Retail Markets
In this study, we investigated the incidence of Cronobacter spp. in seafood collected from retail fish markets of Mumbai, India. A total of 50 samples comprising fresh finfish (n = 32), shellfish (n = 6), dried fish (n = 9) and water (n = 3) were analyzed for Cronobacter spp. by selective enrichment, isolation and biochemical tests. Of 145 isolates presumptively identified as Cronobacter spp. by biochemical tests, 37 were confirmed as Cronobacter spp. by Polymerase Chain Reaction (PCR) specific to the internal transcribed spacer (ITS) regions. Based on the partial ITS gene sequence analysis, 35 isolates were identified as ...
Source: Current Microbiology - March 28, 2021 Category: Microbiology Authors: Sambit K Das Sanath H Kumar Binaya B Nayak Manjusha Lekshmi Source Type: research

Isolation and Identification of Cronobacter spp. from Fish and Shellfish Sold in Retail Markets
In this study, we investigated the incidence of Cronobacter spp. in seafood collected from retail fish markets of Mumbai, India. A total of 50 samples comprising fresh finfish (n = 32), shellfish (n = 6), dried fish (n = 9) and water (n = 3) were analyzed for Cronobacter spp. by selective enrichment, isolation and biochemical tests. Of 145 isolates presumptively identified as Cronobacter spp. by biochemical tests, 37 were confirmed as Cronobacter spp. by Polymerase Chain Reaction (PCR) specific to the internal transcribed spacer (ITS) regions. Based on the partial ITS gene sequence analysis, 35 isolates were identified as ...
Source: Current Microbiology - March 28, 2021 Category: Microbiology Authors: Sambit K Das Sanath H Kumar Binaya B Nayak Manjusha Lekshmi Source Type: research

Impact of Bacillus licheniformis SV1 Derived Glycolipid on Candida glabrata Biofilm
Curr Microbiol. 2021 Mar 27. doi: 10.1007/s00284-021-02461-5. Online ahead of print.ABSTRACTIn the present investigation, we have evaluated the antibiofilm potential of Bacillus licheniformis SV1 derived glycolipid against C. glabrata biofilm. Impact of isolated glycolipid on the viability of C. glabrata and on inhibiting as well as eradicating ability of its biofilm were studied. Further, morphological alterations, reactive oxygen species generation (ROS) production and transcriptional expression of selected genes (RT-PCR) of C. glabrata in response with isolated glycolipid were studied. The isolated glycolipid (1.0 mg ml...
Source: Current Microbiology - March 27, 2021 Category: Microbiology Authors: Sonam Gupta Payal Gupta Vikas Pruthi Source Type: research

Diversity of Endophytic Bacteria in Cardamine hupingshanensis and Potential of Culturable Selenium-Resistant Endophytes to Enhance Seed Germination Under Selenate Stress
Curr Microbiol. 2021 Mar 27. doi: 10.1007/s00284-021-02444-6. Online ahead of print.ABSTRACTThe endophytic bacterial communities of Se hyperaccumulator Cardamine hupingshanensis collected from greenhouse and selenium mining area in Enshi City were investigated by Illumina sequencing technology. In addition, 14 culturable endophytic selenium-resistant strains were isolated and their selenium tolerance and plant growth promotion abilities were studied. The results showed that phylum Proteobacteria predominated in all the plants (> 70%) regardless of their habitats, with most of the OTUs related to Betaproteobacteria, Alph...
Source: Current Microbiology - March 27, 2021 Category: Microbiology Authors: Qi Li Shoubiao Zhou Na Liu Source Type: research

Vigna spp. Root-Nodules Harbor Potentially Pathogenic Fungi Controlled By Co-habiting Bacteria
This study aimed to isolate, identify, and evaluate the pathogenicity of nodule-borne fungi of asymptomatic Vigna spp. plants, grown in soils from preserved tropical dry forests (Caatinga) areas and identify the occurrence of co-habiting bacteria from these plants, and which have potential to control the co-occurring pathogenic fungi. Fungi and bacteria were isolated from three Vigna species (V. unguiculata, V. radiata, and V. mungo), grown in soil samples collected in five preserved Caatinga areas (Northeastern, Brazil). All fungi and selected bacteria were phylogenetically characterized by the sequencing of ITS1-5.8S-ITS...
Source: Current Microbiology - March 27, 2021 Category: Microbiology Authors: Val éria Borges da Silva Cl áudia Silva Gomes Bomfim P âmella Thalita Souza Sena J éssica Caroline Souza Santos Wesley da Silva Mattos Carlos Alberto Tu ão Gava Adailson Pereira de Souza Paulo Ivan Fernandes-J únior Source Type: research

Transcriptome Response of the Tropical Marine Yeast Yarrowia lipolytica on Exposure to Uranium
Curr Microbiol. 2021 Mar 27. doi: 10.1007/s00284-021-02459-z. Online ahead of print.ABSTRACTIn our earlier investigation, we reported the consequences of uranium (U)-induced oxidative stress and cellular defense mechanisms alleviating uranium toxicity in the marine yeast Yarrowia lipolytica NCIM 3589. However, there is lack of information on stress response towards uranium toxicity at molecular level in this organism. To gain an insight on this, transcriptional response of Y. lipolytica after exposure to 50 µM uranium was investigated by RNA sequencing at the global level in this study. The de novo transcriptome anal...
Source: Current Microbiology - March 27, 2021 Category: Microbiology Authors: Nilesh Kolhe Abhijeet Kulkarni Smita Zinjarde Celin Acharya Source Type: research

Impact of Bacillus licheniformis SV1 Derived Glycolipid on Candida glabrata Biofilm
Curr Microbiol. 2021 Mar 27. doi: 10.1007/s00284-021-02461-5. Online ahead of print.ABSTRACTIn the present investigation, we have evaluated the antibiofilm potential of Bacillus licheniformis SV1 derived glycolipid against C. glabrata biofilm. Impact of isolated glycolipid on the viability of C. glabrata and on inhibiting as well as eradicating ability of its biofilm were studied. Further, morphological alterations, reactive oxygen species generation (ROS) production and transcriptional expression of selected genes (RT-PCR) of C. glabrata in response with isolated glycolipid were studied. The isolated glycolipid (1.0 mg ml...
Source: Current Microbiology - March 27, 2021 Category: Microbiology Authors: Sonam Gupta Payal Gupta Vikas Pruthi Source Type: research

Diversity of Endophytic Bacteria in Cardamine hupingshanensis and Potential of Culturable Selenium-Resistant Endophytes to Enhance Seed Germination Under Selenate Stress
Curr Microbiol. 2021 Mar 27. doi: 10.1007/s00284-021-02444-6. Online ahead of print.ABSTRACTThe endophytic bacterial communities of Se hyperaccumulator Cardamine hupingshanensis collected from greenhouse and selenium mining area in Enshi City were investigated by Illumina sequencing technology. In addition, 14 culturable endophytic selenium-resistant strains were isolated and their selenium tolerance and plant growth promotion abilities were studied. The results showed that phylum Proteobacteria predominated in all the plants (> 70%) regardless of their habitats, with most of the OTUs related to Betaproteobacteria, Alph...
Source: Current Microbiology - March 27, 2021 Category: Microbiology Authors: Qi Li Shoubiao Zhou Na Liu Source Type: research

Vigna spp. Root-Nodules Harbor Potentially Pathogenic Fungi Controlled By Co-habiting Bacteria
This study aimed to isolate, identify, and evaluate the pathogenicity of nodule-borne fungi of asymptomatic Vigna spp. plants, grown in soils from preserved tropical dry forests (Caatinga) areas and identify the occurrence of co-habiting bacteria from these plants, and which have potential to control the co-occurring pathogenic fungi. Fungi and bacteria were isolated from three Vigna species (V. unguiculata, V. radiata, and V. mungo), grown in soil samples collected in five preserved Caatinga areas (Northeastern, Brazil). All fungi and selected bacteria were phylogenetically characterized by the sequencing of ITS1-5.8S-ITS...
Source: Current Microbiology - March 27, 2021 Category: Microbiology Authors: Val éria Borges da Silva Cl áudia Silva Gomes Bomfim P âmella Thalita Souza Sena J éssica Caroline Souza Santos Wesley da Silva Mattos Carlos Alberto Tu ão Gava Adailson Pereira de Souza Paulo Ivan Fernandes-J únior Source Type: research

Transcriptome Response of the Tropical Marine Yeast Yarrowia lipolytica on Exposure to Uranium
Curr Microbiol. 2021 Mar 27. doi: 10.1007/s00284-021-02459-z. Online ahead of print.ABSTRACTIn our earlier investigation, we reported the consequences of uranium (U)-induced oxidative stress and cellular defense mechanisms alleviating uranium toxicity in the marine yeast Yarrowia lipolytica NCIM 3589. However, there is lack of information on stress response towards uranium toxicity at molecular level in this organism. To gain an insight on this, transcriptional response of Y. lipolytica after exposure to 50 µM uranium was investigated by RNA sequencing at the global level in this study. The de novo transcriptome anal...
Source: Current Microbiology - March 27, 2021 Category: Microbiology Authors: Nilesh Kolhe Abhijeet Kulkarni Smita Zinjarde Celin Acharya Source Type: research