Genomic Characterization of Two NDM-5-Producing Isolates of Klebsiella pneumoniae ST11 from a Single Patient
Curr Microbiol. 2024 Jan 25;81(3):76. doi: 10.1007/s00284-023-03596-3.ABSTRACTTwo metallo-β-lactamase-producing Klebsiella pneumoniae (HA30 and HA31) were isolated in a hospital in Argentina during 2018. K. pneumoniae HA30 was isolated from a rectal swab during the epidemiological surveillance for carbapenemase-producing strains, while K. pneumoniae HA31 was collected from the same patient 4 days after hospitalization. The aim of the present study was to identify the clonal relationships and resistome of these two NDM-producing K. pneumoniae strains isolated from a patient with a fatal outcome. Whole-genome sequencing (WG...
Source: Current Microbiology - January 24, 2024 Category: Microbiology Authors: Ver ónica E Álvarez Laura Carrera P áez Mar ía Piekar Natalia Garc ía Allende Josefina Campos Nicol ás Mendiondo Andrea Aguilar Barbara Fox Liliana Fern ández-Canigia Mar ía P Quiroga Daniela Centr ón Source Type: research
Multiplex Detection of RNA Viruses Based on Ligation Reaction and Universal PCR Amplification
Curr Microbiol. 2024 Jan 23;81(3):75. doi: 10.1007/s00284-023-03582-9.ABSTRACTTo detect several RNA viruses simultaneously, a method based on multiplex ligation reaction combined with multiplex qPCR or multiplex PCR+capillary electrophoresis was established to detect four RNA viruses: human immunodeficiency virus (HIV), hepatitis C (HCV), influenza A virus (IAV) H1N1 and H5N1. The experimental conditions including ligation probe concentration, annealing procedure, ligation temperature and ligase dosage were optimized intensively. We found that the specificity of the ligation reaction was affected by the probe concentration...
Source: Current Microbiology - January 23, 2024 Category: Microbiology Authors: Lijun Qian Junhua Xiao Kai Li Yuxun Zhou Source Type: research
Description of Mycolicibacterium arenosum sp. nov. Isolated from Coastal Sand on the Yellow Sea Coast
Curr Microbiol. 2024 Jan 23;81(3):73. doi: 10.1007/s00284-023-03587-4.ABSTRACTA Gram-staining-positive, aerobic, non-spore-forming bacterium was isolated from coastal sand samples from Incheon in the Republic of Korea and designated as strain CAU 1645T. The optimum conditions for growth were observed at 30 °C in growth media containing 1% (w/v) NaCl at pH 9.0. The predominant respiratory quinone was MK-9 and the major fatty acids were C16:0, C17:1 w7c, and summed feature 7. Similarly, the 16S rRNA gene sequence exhibited the highest similarity with Mycolicibacterium bacteremicum DSM 45578T and Mycolicibacterium neoaurum J...
Source: Current Microbiology - January 22, 2024 Category: Microbiology Authors: Jiseon Jeong Soyeon Ahn Thoi Cong Truong Jong-Hwa Kim Veeraya Weerawongwiwat Jung-Sook Lee Jung-Hoon Yoon Ampaitip Sukhoom Wonyong Kim Source Type: research
Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus fumigatus
In this study, we investigate the biological impact of Khib in A. fumigatus. Using a combination of antibody enrichment with the conventional LC-MS/MS method, the pattern of Khib-modification in proteins and their respective sites were analyzed in a wild type strain of A. fumigatus. Our findings revealed 3494 Khib-modified proteins with a total of 18,091 modified sites in this strain. Functional enrichment analysis indicated that these Khib-modified proteins participate in a diverse range of cellular functions, spanning various subcellular locations such as ribosome biosynthesis, protein synthesis and nucleocytoplasmic tra...
Source: Current Microbiology - January 22, 2024 Category: Microbiology Authors: Hailin Zheng Huan Mei Xiaofang Li Dongmei Li Weida Liu Source Type: research
Pelagibacterium flavum sp. nov., Isolated from Soil Sample
Curr Microbiol. 2024 Jan 23;81(3):72. doi: 10.1007/s00284-023-03583-8.ABSTRACTA Gram-stain-negative, yellow, moist and circular, aerobic, motile, and rod-shaped bacterium, designated YIM 151497T, was isolated from soil sample collected from Blue-Bridge, Weizhou Island, Guangxi province, China. Classification using a polyphasic approach suggested that strain YIM 151497T belonged to the genus Pelagibacterium, and was closely relevant to Pelagibacterium nitratireducens JLT2005T (98.8%), Pelagibacterium halotolerans CGMCC 1.7692T (98.7%), Pelagibacterium lixinzhangensis H64T (98.1%), and Pelagibacterium luteolum CGMCC 1.10267T...
Source: Current Microbiology - January 22, 2024 Category: Microbiology Authors: Xue-Mei Chen Zu-Zhen Yang Si-Rong He Lian-Shuang Xiong Qing Yuan Shu-Ya Zuo Cheng-Lin Jiang Yi Jiang Source Type: research
Genomic, Physiological, Biochemical, and Phenotypic Evidences Reveal a New Species, Halomicroarcula salaria sp. nov
Curr Microbiol. 2024 Jan 23;81(3):71. doi: 10.1007/s00284-023-03574-9.ABSTRACTAn extremely halophilic archaeon strain named FL173T was isolated from a salt mine (Anhui Province, China). Colonies on agar plate are orange-red, moist, and opaque. Cells are motile, Gram-stain-negative, polymorphic, and lyse in distilled water. Cells are able to grow at temperatures, NaCl concentrations, and pH ranging from 20 to 50 °C (optimum 42 °C), 2.6 to 5.1 M NaCl concentration (optimum 3.4 M), and 5.5 to 9.5 pH (optimum 7.0), respectively. Mg2+ is not necessary for growth. The major polar lipids of strain FL173T were phosphatidylglycer...
Source: Current Microbiology - January 22, 2024 Category: Microbiology Authors: Tao Hong Juntao Ke Liangzhon Chen Yuling Hao Peng Lu Shaoxing Chen Source Type: research
Clinical Characteristics, Treatment, and Outcomes of Veterans with Cerebrospinal Fluid Culture Positive for Gram-Negative Rod Bacteria: A Retrospective Analysis over 18 Years in 125 Veterans Health Administration Hospitals
Curr Microbiol. 2024 Jan 19;81(2):70. doi: 10.1007/s00284-023-03593-6.ABSTRACTOptimal management for patients with bacterial ventriculitis/meningitis due to Gram-negative rods (GNRs) has yet to be well investigated. We assessed the clinical characteristics, treatment, and outcomes of patients with a positive cerebrospinal fluid (CSF) culture for GNRs. We conducted a retrospective cohort study of all patients with a positive CSF culture within the Veterans Health Administration (VHA) system during 2003-2020. Clinical and microbiological characteristics between the true meningitis and contamination groups were compared. Of t...
Source: Current Microbiology - January 19, 2024 Category: Microbiology Authors: Shinya Hasegawa Eiyu Matsumoto Jennifer R Carlson Hiroyuki Suzuki Source Type: research
Variation Among Japanese Miso Breweries in Indoor Microbiomes is Mainly Ascribed to Variation in Type of Indoor Surface
Curr Microbiol. 2024 Jan 18;81(2):68. doi: 10.1007/s00284-023-03591-8.ABSTRACTMiso is a microbially-fermented soybean food. The miso brewery indoor microbiome contributes to miso fermentation. Japanese breweries are not climate-controlled, so indoor spaces are strongly affected by the prevailing climate. Because climate influences microorganism distribution, our first hypothesis is that latitude, as a proxy for climate, is a major determinant of brewery indoor microbiome structure. Breweries vary in interior surface materials and in the way operations (steaming, processing, fermenting) are apportioned among rooms. Therefor...
Source: Current Microbiology - January 18, 2024 Category: Microbiology Authors: Roger T Koide Makoto Kanauchi Yasushi Hashimoto Source Type: research
Immobilization of Thermoplasma acidophilum Glucose Dehydrogenase and Isocitrate Dehydrogenase Through Enzyme-Inorganic Hybrid Nanocrystal Formation
In conclusion, this study represents the first immobilization of TaGDH and TaIDH using the hybrid nanocrystal forming method. Furthermore, we achieved significant activity enhancement of TaIDH through immobilization and demonstrated the recyclability of the immobilized enzymes.PMID:38236425 | PMC:PMC10796475 | DOI:10.1007/s00284-023-03577-6 (Source: Current Microbiology)
Source: Current Microbiology - January 18, 2024 Category: Microbiology Authors: Shusuke Oshima Yuri Oku Kotchakorn T Sriwong Yutaro Kimura Tomoko Matsuda Source Type: research
Common Features of Environmental Mycobacterium chelonae from Colorado Using Partial and Whole Genomic Sequence Analyses
Curr Microbiol. 2024 Jan 18;81(2):69. doi: 10.1007/s00284-023-03589-2.ABSTRACTNontuberculous mycobacteria (NTM) are environmentally acquired opportunistic pathogens that cause chronic lung disease in susceptible individuals. While presumed to be ubiquitous in built and natural environments, NTM environmental studies are limited. While environmental sampling campaigns have been performed in geographic areas of high NTM disease burden, NTM species diversity is less defined among areas of lower disease burden like Colorado. In Colorado, metals such as molybdenum have been correlated with increased risk for NTM infection, yet ...
Source: Current Microbiology - January 18, 2024 Category: Microbiology Authors: Kayden G Glauser Reagan E Kelley William J Leonard Jo Hendrix Suzanne Petri Eric I Tong Yvonne L Chan Ettie M Lipner Stephanie N Dawrs Jennifer R Honda Source Type: research
Comparative Analyses of the Symbiotic Associations of the Host Paramecium bursaria with Free-Living and Native Symbiotic Species of Chlorella
Curr Microbiol. 2024 Jan 17;81(2):66. doi: 10.1007/s00284-023-03590-9.ABSTRACTEach symbiotic Chlorella variabilis associated with the ciliate Paramecium bursaria is enclosed in a symbiosome called the perialgal vacuole. Various potential symbionts, such as bacteria, yeasts, other algae, and free-living Chlorella spp., can infect P. bursaria. However, the detailed infection process of each of them in algae-free P. bursaria is unknown. Here, we aimed to elucidate the difference of the infection process between the free-living C. sorokiniana strain NIES-2169 and native symbiotic C. variabilis strain 1N. We investigated the fa...
Source: Current Microbiology - January 17, 2024 Category: Microbiology Authors: Yuuki Kodama Yuuka Endoh Source Type: research
Effects of Vancomycin Subtherapeutic Concentration on Staphylococcus aureus Isolated from Hemodialysis Patients with Low Serum Trough Concentrations
In conclusion, our findings suggest that the subtherapeutic concentration of vancomycin decrease S. aureus susceptibility to vancomycin and daptomycin and increases its survival to whole blood bactericidal action.PMID:38231442 | DOI:10.1007/s00284-023-03588-3 (Source: Current Microbiology)
Source: Current Microbiology - January 17, 2024 Category: Microbiology Authors: Rodrigo Alto é Chagas Cristiana de Souza Silva J éssica de Cássia Teixeira Birro K ênia Valéria Dos Santos Source Type: research
Co-Occurrence Patterns of Soil Fungal and Bacterial Communities in Subtropical Forest-Transforming Areas
This study provides new insights into our understanding of the feedback of underground creatures to forest conversion and highlights the importance of microbial modules in the nutrient cycling process.PMID:38225342 | DOI:10.1007/s00284-023-03608-2 (Source: Current Microbiology)
Source: Current Microbiology - January 15, 2024 Category: Microbiology Authors: Yajun Liu Xin Jin Shihao Huang Yizhen Liu Zhaoyu Kong Lan Wu Gang Ge Source Type: research
Genetic Determinants in MRSA Carriage and Their Association with Decolonization Outcome
In conclusion, this explorative study showed a higher eradication failure rate in complicated MRSA carriers with ciprofloxacin-resistant MRSA lineages, which are predominantly healthcare-associated. Further studies are warranted to confirm the higher eradication failure risk of ciprofloxacin-resistant lineages, and identify the underlying mechanisms.PMID:38217690 | PMC:PMC10787693 | DOI:10.1007/s00284-023-03581-w (Source: Current Microbiology)
Source: Current Microbiology - January 13, 2024 Category: Microbiology Authors: Annette C Westgeest Emile F Schippers Sigrid Rosema Monika A Fliss Ed J Kuijper Romy D Zwittink Mari ëtte Lokate Marjan Wouthuyzen-Bakker Merel M C Lambregts Erik Bathoorn Source Type: research
Bacillus licheniformis M2-7 Decreases Ochratoxin A Concentrations in Coffee Beans During Storage
In this study, coffee beans (Coffea arabica L.) were inoculated with Bacillus licheniformis M2-7, after which we monitored fungal growth, in vitro antagonism, and OTA concentration. Our findings demonstrated that coffee beans inoculated with this bacterial strain exhibited a significant decrease in fungal populations belonging to the genera Aspergillus and Penicillium, which are known to produce OTA. Moreover, strain M2-7 decreased the growth rates of these fungi from 67.8% to 95.5% (P < 0.05). Similarly, inoculation with B. licheniformis strain M2-7 effectively reduced the OTA concentration from 24.35 ± 1.61 to 5.52 ...
Source: Current Microbiology - January 12, 2024 Category: Microbiology Authors: Mar ía Rojas-Pablo Erubiel Toledo-Hern ández Miguel A Rodr íguez-Barrera Jeiry Toribio-Jim énez Carmina Torreblanca-Ram írez V íctor Manuel Rosas-Guerrero Sergio Adri án Salgado-Souto Patricia Álvarez-Fitz Alejandro Bola ños-Dircio Yanet Romero-R Source Type: research
