Three New Species of Laccaria (Hydnangiaceae) from India (Darjeeling Hills) Based on Molecular and Morphological Evidence
Curr Microbiol. 2024 Jan 28;81(3):79. doi: 10.1007/s00284-023-03598-1.ABSTRACTThree new species of Laccaria infundibuliformis, L. pallidus, and L. darjeelingensis, collected from Darjeeling, India, are described based on morphological and molecular evidence. Laccaria infundibuliformis is characterized by its small infundibuliform basidiocarps, and echinulate basidiospores with spines up to 1.36 µm long. Laccaria pallidus is characterized by medium-sized greyish-red basidiocarps, and echinulate basidiospores with spines up to 1.9 µm long. Laccaria darjeelingensis is characterized by dull red basidiocarps, and echinulate b...
Source: Current Microbiology - January 28, 2024 Category: Microbiology Authors: Alisha Thapa Juna Tamang Krishnendu Acharya Source Type: research

Nakazawaea tricholomae f.a., sp. nov., a Novel Ascomycetous Yeast Species Isolated from Two Mushroom Species in China
Curr Microbiol. 2024 Jan 28;81(3):78. doi: 10.1007/s00284-023-03600-w.ABSTRACTTwo yeast strains designated as 20-27-1 and 20-28 were isolated from the fruiting bodies of Tricholoma gambosum and Marasmius maximus, respectively, which were collected in Wudaogou, Weichang county, Chengde area, Hebei Province, China. The multi-locus analysis of the sequences of the rDNA ITS, D1/D2 LSU, and SSU regions, together with partial sequences of two protein-coding genes RPB1 and TEF1 indicates that the two strains are closely related to Nakazawaea ernobii and Nakazawaea holstii, showing the similarity values of 99.3-98.7%, 97.2-97.1%, ...
Source: Current Microbiology - January 28, 2024 Category: Microbiology Authors: Min Liu You-Liang Jiang Yu-Xuan Zhang Qi-Ming Wang Source Type: research

Mutational analysis of the transmembrane α4-helix of Bacillus thuringiensis mosquito-larvicidal Cry4Aa toxin
Curr Microbiol. 2024 Jan 28;81(3):80. doi: 10.1007/s00284-023-03602-8.ABSTRACTCry4Aa, produced by Bacillus thuringiensis subsp. israelensis, exhibits specific toxicity to larvae of medically important mosquito genera. Cry4Aa functions as a pore-forming toxin, and a helical hairpin (α4-loop-α5) of domain I is believed to be the transmembrane domain that forms toxin pores. Pore formation is considered to be a central mode of Cry4Aa action, but the relationship between pore formation and toxicity is poorly understood. In the present study, we constructed Cry4Aa mutants in which each polar amino acid residues within the tran...
Source: Current Microbiology - January 28, 2024 Category: Microbiology Authors: Hirokazu Takahashi Mami Asakura Toru Ide Tohru Hayakawa Source Type: research

Effective Inhibition of Listeria monocytogenes Biofilm Formation by Satureja rechingeri Essential Oil: Mechanisms and Implications
In this study, we investigated the inhibitory potential of Satureja rechingeri essential oil (Sr-EO) against L. monocytogenes growth and biofilm formation. Gas chromatography-mass spectrometry analysis revealed a high carvacrol content in Sr-EO, a compound with known antimicrobial properties. We examined the effects of Sr-EO on initial attachment and preformed biofilms, using crystal violet and MTT assays to quantify attached biomass and metabolic activity, respectively. Our results demonstrated that Sr-EO not only prevented initial attachment but also effectively disrupted preformed biofilms, indicating its potential as a...
Source: Current Microbiology - January 27, 2024 Category: Microbiology Authors: Siavash Maktabi Roya Rashnavadi Mohammad Reza Tabandeh Mohammad Mahmoodi Sourestani Source Type: research

Genomic Characterization of Two NDM-5-Producing Isolates of Klebsiella pneumoniae ST11 from a Single Patient
Curr Microbiol. 2024 Jan 25;81(3):76. doi: 10.1007/s00284-023-03596-3.ABSTRACTTwo metallo-β-lactamase-producing Klebsiella pneumoniae (HA30 and HA31) were isolated in a hospital in Argentina during 2018. K. pneumoniae HA30 was isolated from a rectal swab during the epidemiological surveillance for carbapenemase-producing strains, while K. pneumoniae HA31 was collected from the same patient 4 days after hospitalization. The aim of the present study was to identify the clonal relationships and resistome of these two NDM-producing K. pneumoniae strains isolated from a patient with a fatal outcome. Whole-genome sequencing (WG...
Source: Current Microbiology - January 24, 2024 Category: Microbiology Authors: Ver ónica E Álvarez Laura Carrera P áez Mar ía Piekar Natalia Garc ía Allende Josefina Campos Nicol ás Mendiondo Andrea Aguilar Barbara Fox Liliana Fern ández-Canigia Mar ía P Quiroga Daniela Centr ón Source Type: research

Multiplex Detection of RNA Viruses Based on Ligation Reaction and Universal PCR Amplification
Curr Microbiol. 2024 Jan 23;81(3):75. doi: 10.1007/s00284-023-03582-9.ABSTRACTTo detect several RNA viruses simultaneously, a method based on multiplex ligation reaction combined with multiplex qPCR or multiplex PCR+capillary electrophoresis was established to detect four RNA viruses: human immunodeficiency virus (HIV), hepatitis C (HCV), influenza A virus (IAV) H1N1 and H5N1. The experimental conditions including ligation probe concentration, annealing procedure, ligation temperature and ligase dosage were optimized intensively. We found that the specificity of the ligation reaction was affected by the probe concentration...
Source: Current Microbiology - January 23, 2024 Category: Microbiology Authors: Lijun Qian Junhua Xiao Kai Li Yuxun Zhou Source Type: research

Description of Mycolicibacterium arenosum sp. nov. Isolated from Coastal Sand on the Yellow Sea Coast
Curr Microbiol. 2024 Jan 23;81(3):73. doi: 10.1007/s00284-023-03587-4.ABSTRACTA Gram-staining-positive, aerobic, non-spore-forming bacterium was isolated from coastal sand samples from Incheon in the Republic of Korea and designated as strain CAU 1645T. The optimum conditions for growth were observed at 30 °C in growth media containing 1% (w/v) NaCl at pH 9.0. The predominant respiratory quinone was MK-9 and the major fatty acids were C16:0, C17:1 w7c, and summed feature 7. Similarly, the 16S rRNA gene sequence exhibited the highest similarity with Mycolicibacterium bacteremicum DSM 45578T and Mycolicibacterium neoaurum J...
Source: Current Microbiology - January 22, 2024 Category: Microbiology Authors: Jiseon Jeong Soyeon Ahn Thoi Cong Truong Jong-Hwa Kim Veeraya Weerawongwiwat Jung-Sook Lee Jung-Hoon Yoon Ampaitip Sukhoom Wonyong Kim Source Type: research

Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus fumigatus
In this study, we investigate the biological impact of Khib in A. fumigatus. Using a combination of antibody enrichment with the conventional LC-MS/MS method, the pattern of Khib-modification in proteins and their respective sites were analyzed in a wild type strain of A. fumigatus. Our findings revealed 3494 Khib-modified proteins with a total of 18,091 modified sites in this strain. Functional enrichment analysis indicated that these Khib-modified proteins participate in a diverse range of cellular functions, spanning various subcellular locations such as ribosome biosynthesis, protein synthesis and nucleocytoplasmic tra...
Source: Current Microbiology - January 22, 2024 Category: Microbiology Authors: Hailin Zheng Huan Mei Xiaofang Li Dongmei Li Weida Liu Source Type: research

Pelagibacterium flavum sp. nov., Isolated from Soil Sample
Curr Microbiol. 2024 Jan 23;81(3):72. doi: 10.1007/s00284-023-03583-8.ABSTRACTA Gram-stain-negative, yellow, moist and circular, aerobic, motile, and rod-shaped bacterium, designated YIM 151497T, was isolated from soil sample collected from Blue-Bridge, Weizhou Island, Guangxi province, China. Classification using a polyphasic approach suggested that strain YIM 151497T belonged to the genus Pelagibacterium, and was closely relevant to Pelagibacterium nitratireducens JLT2005T (98.8%), Pelagibacterium halotolerans CGMCC 1.7692T (98.7%), Pelagibacterium lixinzhangensis H64T (98.1%), and Pelagibacterium luteolum CGMCC 1.10267T...
Source: Current Microbiology - January 22, 2024 Category: Microbiology Authors: Xue-Mei Chen Zu-Zhen Yang Si-Rong He Lian-Shuang Xiong Qing Yuan Shu-Ya Zuo Cheng-Lin Jiang Yi Jiang Source Type: research

Genomic, Physiological, Biochemical, and Phenotypic Evidences Reveal a New Species, Halomicroarcula salaria sp. nov
Curr Microbiol. 2024 Jan 23;81(3):71. doi: 10.1007/s00284-023-03574-9.ABSTRACTAn extremely halophilic archaeon strain named FL173T was isolated from a salt mine (Anhui Province, China). Colonies on agar plate are orange-red, moist, and opaque. Cells are motile, Gram-stain-negative, polymorphic, and lyse in distilled water. Cells are able to grow at temperatures, NaCl concentrations, and pH ranging from 20 to 50 °C (optimum 42 °C), 2.6 to 5.1 M NaCl concentration (optimum 3.4 M), and 5.5 to 9.5 pH (optimum 7.0), respectively. Mg2+ is not necessary for growth. The major polar lipids of strain FL173T were phosphatidylglycer...
Source: Current Microbiology - January 22, 2024 Category: Microbiology Authors: Tao Hong Juntao Ke Liangzhon Chen Yuling Hao Peng Lu Shaoxing Chen Source Type: research

Clinical Characteristics, Treatment, and Outcomes of Veterans with Cerebrospinal Fluid Culture Positive for Gram-Negative Rod Bacteria: A Retrospective Analysis over 18 Years in 125 Veterans Health Administration Hospitals
Curr Microbiol. 2024 Jan 19;81(2):70. doi: 10.1007/s00284-023-03593-6.ABSTRACTOptimal management for patients with bacterial ventriculitis/meningitis due to Gram-negative rods (GNRs) has yet to be well investigated. We assessed the clinical characteristics, treatment, and outcomes of patients with a positive cerebrospinal fluid (CSF) culture for GNRs. We conducted a retrospective cohort study of all patients with a positive CSF culture within the Veterans Health Administration (VHA) system during 2003-2020. Clinical and microbiological characteristics between the true meningitis and contamination groups were compared. Of t...
Source: Current Microbiology - January 19, 2024 Category: Microbiology Authors: Shinya Hasegawa Eiyu Matsumoto Jennifer R Carlson Hiroyuki Suzuki Source Type: research

Variation Among Japanese Miso Breweries in Indoor Microbiomes is Mainly Ascribed to Variation in Type of Indoor Surface
Curr Microbiol. 2024 Jan 18;81(2):68. doi: 10.1007/s00284-023-03591-8.ABSTRACTMiso is a microbially-fermented soybean food. The miso brewery indoor microbiome contributes to miso fermentation. Japanese breweries are not climate-controlled, so indoor spaces are strongly affected by the prevailing climate. Because climate influences microorganism distribution, our first hypothesis is that latitude, as a proxy for climate, is a major determinant of brewery indoor microbiome structure. Breweries vary in interior surface materials and in the way operations (steaming, processing, fermenting) are apportioned among rooms. Therefor...
Source: Current Microbiology - January 18, 2024 Category: Microbiology Authors: Roger T Koide Makoto Kanauchi Yasushi Hashimoto Source Type: research

Immobilization of Thermoplasma acidophilum Glucose Dehydrogenase and Isocitrate Dehydrogenase Through Enzyme-Inorganic Hybrid Nanocrystal Formation
In conclusion, this study represents the first immobilization of TaGDH and TaIDH using the hybrid nanocrystal forming method. Furthermore, we achieved significant activity enhancement of TaIDH through immobilization and demonstrated the recyclability of the immobilized enzymes.PMID:38236425 | PMC:PMC10796475 | DOI:10.1007/s00284-023-03577-6 (Source: Current Microbiology)
Source: Current Microbiology - January 18, 2024 Category: Microbiology Authors: Shusuke Oshima Yuri Oku Kotchakorn T Sriwong Yutaro Kimura Tomoko Matsuda Source Type: research

Common Features of Environmental Mycobacterium chelonae from Colorado Using Partial and Whole Genomic Sequence Analyses
Curr Microbiol. 2024 Jan 18;81(2):69. doi: 10.1007/s00284-023-03589-2.ABSTRACTNontuberculous mycobacteria (NTM) are environmentally acquired opportunistic pathogens that cause chronic lung disease in susceptible individuals. While presumed to be ubiquitous in built and natural environments, NTM environmental studies are limited. While environmental sampling campaigns have been performed in geographic areas of high NTM disease burden, NTM species diversity is less defined among areas of lower disease burden like Colorado. In Colorado, metals such as molybdenum have been correlated with increased risk for NTM infection, yet ...
Source: Current Microbiology - January 18, 2024 Category: Microbiology Authors: Kayden G Glauser Reagan E Kelley William J Leonard Jo Hendrix Suzanne Petri Eric I Tong Yvonne L Chan Ettie M Lipner Stephanie N Dawrs Jennifer R Honda Source Type: research

Comparative Analyses of the Symbiotic Associations of the Host Paramecium bursaria with Free-Living and Native Symbiotic Species of Chlorella
Curr Microbiol. 2024 Jan 17;81(2):66. doi: 10.1007/s00284-023-03590-9.ABSTRACTEach symbiotic Chlorella variabilis associated with the ciliate Paramecium bursaria is enclosed in a symbiosome called the perialgal vacuole. Various potential symbionts, such as bacteria, yeasts, other algae, and free-living Chlorella spp., can infect P. bursaria. However, the detailed infection process of each of them in algae-free P. bursaria is unknown. Here, we aimed to elucidate the difference of the infection process between the free-living C. sorokiniana strain NIES-2169 and native symbiotic C. variabilis strain 1N. We investigated the fa...
Source: Current Microbiology - January 17, 2024 Category: Microbiology Authors: Yuuki Kodama Yuuka Endoh Source Type: research