PCR Development for Analysis of Some Type II Toxin-Antitoxin Systems, relJK, mazEF3, and vapBC3 Genes, in Mycobacterium tuberculosis and Mycobacterium bovis
Curr Microbiol. 2024 Feb 5;81(3):90. doi: 10.1007/s00284-023-03599-0.ABSTRACTToxin-Antitoxin (TA) systems are some small genetic modules in bacteria that play significant roles in resistance and tolerance development to antibiotics. Whole genome sequencing (WGS) is an effective method to analyze TA systems in pathogenic Mycobacteria. However, this study aimed to use a simple and inexpensive PCR-Sequencing approach to investigate the type II TA system. Using data from the WGS of Mycobacterium tuberculosis (M. tuberculosis) strain H37Rv and Mycobacterium bovis (M. bovis) strain BCG, primers specific to the relJK, mazEF3, and...
Source: Current Microbiology - February 4, 2024 Category: Microbiology Authors: Maryam Shafipour Abdolmajid Mohammadzadeh Ezzat Allah Ghaemi Pezhman Mahmoodi Source Type: research
Co-inoculation of Soybean Seedling with Trichoderma asperellum and Irpex laceratus Promotes the Absorption of Nitrogen and Phosphorus
Curr Microbiol. 2024 Feb 5;81(3):87. doi: 10.1007/s00284-023-03571-y.ABSTRACTSoybean are one of the main oil crops in the world. The study demonstrated that co-inoculation with Trichoderma asperellum (Sordariomycetes, Hypocreomycetidae) and Irpex laceratus (Basidiomycota, Polyporales) isolated from Kosteletzkya virginica can promote the growth of soybean seedlings. The two fungi were found to produce various enzymes, including cellulase, amylase, laccase, protease, and urease. Upon inoculation, T. asperellum mainly colonized within the phloem of the roots in soybean seedlings, while I. laceratus mainly in the xylem and phl...
Source: Current Microbiology - February 4, 2024 Category: Microbiology Authors: Zengyuan Tian Xiaomin Wang Yanyi Li Yu Xi Mengting He Yuqi Guo Source Type: research
Putative Role of Anti-microbial Peptide Recovered from Lactiplantibacillus spp. in Biocontrol Activity
In conclusion, this study lays the foundation for utilizing Lactiplantibacillus spp. IT derived AMPs in plant biocontrol strategies, showcasing their efficacy against bacterial phytopathogens. These findings contribute valuable insights for advancing sustainable agricultural practices.PMID:38311656 | DOI:10.1007/s00284-023-03586-5 (Source: Current Microbiology)
Source: Current Microbiology - February 4, 2024 Category: Microbiology Authors: Ishan Tiwari Ali Asger Bhojiya Ram Prasad Shalini Porwal Ajit Varma Devendra Kumar Choudhary Source Type: research
Enhancing Immunological Memory: Unveiling Booster Doses to Bolster Vaccine Efficacy Against Evolving SARS-CoV-2 Mutant Variants
Curr Microbiol. 2024 Feb 5;81(3):91. doi: 10.1007/s00284-023-03597-2.ABSTRACTA growing number of re-infections with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in previously immunized individuals has sparked discussions about the potential need for a booster vaccine dosage to counteract declining antibody levels and new strains. The protective immunity produced by vaccinations, and past illnesses relies on immunological memory. CD4 + T cells, CD8 + T cells, B cells, and long-lasting antibody responses are all components of the adaptive immune system that can generate and maintain this immunological memory....
Source: Current Microbiology - February 4, 2024 Category: Microbiology Authors: Sovan Samanta Jhimli Banerjee Aparna Das Sourav Das Rubai Ahmed Swarnali Das Amitava Pal Kazi Monjur Ali Rupanjan Mukhopadhyay Biplab Giri Sandeep Kumar Dash Source Type: research
Transcription Needs Translation Initiation of the Downstream Gene to Continue Downstream at Intercistronic Junctions in E. Coli
This study verified that the chimeric protein was produced, but unexpectedly, we found that the GalT protein was also synthesized in the mutant, and its amount equaled that in the wild-type. In the wild-type, we also found that the GalE-GalT chimeric protein was produced in an amount equal to that of the GalE protein. These results suggest that translation termination of galE and translation initiation of galT occur independently, thus, corroborating our transcription-translation model: At the cistron junction, transcription, decoupled from translation due to the translation termination of galE, needs translation initiatio...
Source: Current Microbiology - February 4, 2024 Category: Microbiology Authors: Heung Jin Jeon N Monford Paul Abishek Yonho Lee Jeongok Park Heon M Lim Source Type: research
Effects and mechanisms of Salmonella plasmid virulence gene spv on host-regulated cell death
Curr Microbiol. 2024 Feb 2;81(3):86. doi: 10.1007/s00284-024-03612-0.ABSTRACTSalmonella is responsible for the majority of food poisoning outbreaks around the world. Pathogenic Salmonella mostly carries a virulence plasmid that contains the Salmonella plasmid virulence gene (spv), a highly conserved sequence encoding effector proteins that can manipulate host cells. Intestinal epithelial cells are crucial components of the innate immune system, acting as the first barrier of defense against infection. When the barrier is breached, Salmonella encounters the underlying macrophages in lamina propria, triggering inflammation a...
Source: Current Microbiology - February 2, 2024 Category: Microbiology Authors: Li Kang Jiayi You Yuanyuan Li Rui Huang Shuyan Wu Source Type: research
Parendozoicomonas callyspongiae sp. nov. Isolated from a Marine Sponge, Callyspongia elongate, and Reclassification of Sansalvadorimonas verongulae as Parendozoicomonas verongulae comb. nov
Curr Microbiol. 2024 Feb 1;81(3):85. doi: 10.1007/s00284-023-03585-6.ABSTRACTA strictly aerobic Gram-negative bacterium, designated 2012CJ34-2T, was isolated from marine sponge to Chuja-do in Jeju-island, Republic of Korea and taxonomically characterized. Cells were catalase- and oxidase-positive, and non-motile rods (without flagella). Growth was observed at 15-42 °C (optimum, 30 °C), pH 6-9 (optimum, pH 7), and in the presence of 0.5-10% (w/v) NaCl (optimum, 2-3%). The major cellular fatty acid and respiratory quinones were identified summed feature 3 (C16:1 ω7c/C16:1 ω6c), and Q-8 and Q-9, respectively. The polar li...
Source: Current Microbiology - February 1, 2024 Category: Microbiology Authors: Soo-Bin Kim Kyung Hyun Kim Jin-Sook Park Source Type: research
Isolation and Screening of Zn (Zn) Solubilizing Rhizosphere Bacteria from Different Vegetations for Their Ability to Improve Growth, Zn Uptake, and Expression of Zn Transporter Genes in Tomato
In conclusion, both strains can be considered as potential zinc-solubilizing bioinoculants to promote the growth and production yield of tomato.PMID:38294556 | DOI:10.1007/s00284-023-03610-8 (Source: Current Microbiology)
Source: Current Microbiology - January 31, 2024 Category: Microbiology Authors: Attequr Rahman Mian Afaq Ahmad Shiraz Mehmood Abdur Rauf Aqib Iqbal Bakhtiar Ali Mohib Ullah Murad Ali Heba I Mohamed Israr Uddin Source Type: research
Prospecting the Potential of Plant Growth-Promoting Microorganisms for Mitigating Drought Stress in Crop Plants
Curr Microbiol. 2024 Jan 31;81(3):84. doi: 10.1007/s00284-023-03606-4.ABSTRACTDrought is a global phenomenon affecting plant growth and productivity, the severity of which has impacts around the whole world. A number of approaches, such as agronomic, conventional breeding, and genetic engineering, are followed to increase drought resilience; however, they are often time consuming and non-sustainable. Plant growth-promoting microorganisms are used worldwide to mitigate drought stress in crop plants. These microorganisms exhibit multifarious traits, which not only help in improving plant and soil health, but also demonstrate...
Source: Current Microbiology - January 31, 2024 Category: Microbiology Authors: Devendra Singh Shobit Thapa Jyoti Prakash Singh Himanshu Mahawar Anil Kumar Saxena Sunil Kumar Singh Hans Raj Mahla Mahipal Choudhary Manoj Parihar Khushwant Babal Choudhary Hillol Chakdar Source Type: research
The Reasons Behind Long-Term Endemicity of Brucellosis in Low and Middle-Income Countries: Challenges and Future Perspectives
Curr Microbiol. 2024 Jan 30;81(3):82. doi: 10.1007/s00284-023-03605-5.ABSTRACTBrucellosis is a neglected worldwide zoonotic disease with more than 500,000 new human cases each year. Direct contact with infected animals and consumption of undercooked animal origin foods are the main routes of brucellosis transmission to humans. Although long endeavor has been applied to control and eliminate brucellosis from animal and human populations in developing countries especially in low- and middle-income countries (LMICs), the disease is still endemic in these regions. Many common or unique factors including raw milk consumption, u...
Source: Current Microbiology - January 30, 2024 Category: Microbiology Authors: Assadullah Samadi Mirwais Amiri Nabil Hailat Source Type: research
Identifying Fast and Slow-Acting Antimalarial Compounds of Pandemic Response Box Against Blood-Stage Culture of Plasmodium falciparum 3D7
In this study, we assessed the timing of action, either fast or slow-acting of 13 potent compounds of Pandemic Response Box (PRB) against blood-stage Pf3D7 strain by SYBR Green-I assay. The asynchronous culture of Pf3D7 was exposed to varying concentrations of 13 compounds, and IC50 values were determined at 12, 24, 48, 72, and 96 h. We identified four fast-acting compounds (MMV000008, MMV1593541, MMV020752, MMV396785) with rapid-growth inhibitory activity having IC50 values ≤ 0.3 µM at 12 and 24 h. Similarly, we determined nine slow-acting compounds (MMV159340, MMV1634492, MMV1581558, MMV689758, MMV1593540, MMV394033, ...
Source: Current Microbiology - January 30, 2024 Category: Microbiology Authors: Vinoth Rajendran Nimitha Cherthedath Naveen Source Type: research
Three New Species of Laccaria (Hydnangiaceae) from India (Darjeeling Hills) Based on Molecular and Morphological Evidence
Curr Microbiol. 2024 Jan 28;81(3):79. doi: 10.1007/s00284-023-03598-1.ABSTRACTThree new species of Laccaria infundibuliformis, L. pallidus, and L. darjeelingensis, collected from Darjeeling, India, are described based on morphological and molecular evidence. Laccaria infundibuliformis is characterized by its small infundibuliform basidiocarps, and echinulate basidiospores with spines up to 1.36 µm long. Laccaria pallidus is characterized by medium-sized greyish-red basidiocarps, and echinulate basidiospores with spines up to 1.9 µm long. Laccaria darjeelingensis is characterized by dull red basidiocarps, and echinulate b...
Source: Current Microbiology - January 28, 2024 Category: Microbiology Authors: Alisha Thapa Juna Tamang Krishnendu Acharya Source Type: research
Nakazawaea tricholomae f.a., sp. nov., a Novel Ascomycetous Yeast Species Isolated from Two Mushroom Species in China
Curr Microbiol. 2024 Jan 28;81(3):78. doi: 10.1007/s00284-023-03600-w.ABSTRACTTwo yeast strains designated as 20-27-1 and 20-28 were isolated from the fruiting bodies of Tricholoma gambosum and Marasmius maximus, respectively, which were collected in Wudaogou, Weichang county, Chengde area, Hebei Province, China. The multi-locus analysis of the sequences of the rDNA ITS, D1/D2 LSU, and SSU regions, together with partial sequences of two protein-coding genes RPB1 and TEF1 indicates that the two strains are closely related to Nakazawaea ernobii and Nakazawaea holstii, showing the similarity values of 99.3-98.7%, 97.2-97.1%, ...
Source: Current Microbiology - January 28, 2024 Category: Microbiology Authors: Min Liu You-Liang Jiang Yu-Xuan Zhang Qi-Ming Wang Source Type: research
Mutational analysis of the transmembrane α4-helix of Bacillus thuringiensis mosquito-larvicidal Cry4Aa toxin
Curr Microbiol. 2024 Jan 28;81(3):80. doi: 10.1007/s00284-023-03602-8.ABSTRACTCry4Aa, produced by Bacillus thuringiensis subsp. israelensis, exhibits specific toxicity to larvae of medically important mosquito genera. Cry4Aa functions as a pore-forming toxin, and a helical hairpin (α4-loop-α5) of domain I is believed to be the transmembrane domain that forms toxin pores. Pore formation is considered to be a central mode of Cry4Aa action, but the relationship between pore formation and toxicity is poorly understood. In the present study, we constructed Cry4Aa mutants in which each polar amino acid residues within the tran...
Source: Current Microbiology - January 28, 2024 Category: Microbiology Authors: Hirokazu Takahashi Mami Asakura Toru Ide Tohru Hayakawa Source Type: research
Effective Inhibition of Listeria monocytogenes Biofilm Formation by Satureja rechingeri Essential Oil: Mechanisms and Implications
In this study, we investigated the inhibitory potential of Satureja rechingeri essential oil (Sr-EO) against L. monocytogenes growth and biofilm formation. Gas chromatography-mass spectrometry analysis revealed a high carvacrol content in Sr-EO, a compound with known antimicrobial properties. We examined the effects of Sr-EO on initial attachment and preformed biofilms, using crystal violet and MTT assays to quantify attached biomass and metabolic activity, respectively. Our results demonstrated that Sr-EO not only prevented initial attachment but also effectively disrupted preformed biofilms, indicating its potential as a...
Source: Current Microbiology - January 27, 2024 Category: Microbiology Authors: Siavash Maktabi Roya Rashnavadi Mohammad Reza Tabandeh Mohammad Mahmoodi Sourestani Source Type: research
