Genome-Based Reclassification of Anoxybacillus geothermalis Filippidou et al. 2016 as a Later Heterotypic Synonym of Anoxybacillus rupiensis Derekova et al. 2007
In this study, our aim was to elucidate the relationship between Anoxybacillus rupiensis DSM 17127T and Anoxybacillus geothermalis GSsed3T through whole-genome phylogenetic analysis. The obtained 16S rRNA gene sequence from the genome of A. rupiensis DSM 17127T exhibited a 99.8% similarity with A. geothermalis GSsed3T. In the phylogenetic trees constructed using whole-genome sequences and 16S rRNA gene sequences, A. rupiensis DSM 17127T and A. geothermalis GSsed3T were observed to form a clade, indicating a close relationship between them. Moreover, the average amino acid identity, average nucleotide identity, and digital ...
Source: Current Microbiology - February 20, 2024 Category: Microbiology Authors: Kadriye Inan Bektas Aleyna Nalcaoglu Halil İbrahim Guler Sabriye Canakci Ali Osman Belduz Source Type: research
Exploring the Hot Springs of Golan: A Source of Thermophilic Bacteria and Enzymes with Industrial Promise
This study screens and isolates thermophilic bacteria and thermozymes from the Golan hot springs in Karakocan, Elazig, Turkey. The study also characterizes thermophilic bacteria and their thermozymes to understand their features and applications better. Golan hot spring water samples at 50 °C yielded 12 isolates. GKE 02, 07, 08, and 10 produce amylase, GKE 04, 08, and 11 cellulase, and GKE 06 xylanase. One isolate (GKE 08) displayed both amylolytic and cellulolytic activity on agar plates. GKE 02 had the highest plate assay amylolytic index (2.3) and amylase activity (67.87 U/ml). Plate assay indicates GKE 08 has 1.5 amyl...
Source: Current Microbiology - February 20, 2024 Category: Microbiology Authors: Song ül Yaşar Yildiz Source Type: research
Genome-Based Reclassification of Anoxybacillus geothermalis Filippidou et al. 2016 as a Later Heterotypic Synonym of Anoxybacillus rupiensis Derekova et al. 2007
In this study, our aim was to elucidate the relationship between Anoxybacillus rupiensis DSM 17127T and Anoxybacillus geothermalis GSsed3T through whole-genome phylogenetic analysis. The obtained 16S rRNA gene sequence from the genome of A. rupiensis DSM 17127T exhibited a 99.8% similarity with A. geothermalis GSsed3T. In the phylogenetic trees constructed using whole-genome sequences and 16S rRNA gene sequences, A. rupiensis DSM 17127T and A. geothermalis GSsed3T were observed to form a clade, indicating a close relationship between them. Moreover, the average amino acid identity, average nucleotide identity, and digital ...
Source: Current Microbiology - February 20, 2024 Category: Microbiology Authors: Kadriye Inan Bektas Aleyna Nalcaoglu Halil İbrahim Guler Sabriye Canakci Ali Osman Belduz Source Type: research
Psychromicrobium Xiongbiense sp. nov., an Actinobacterium Isolated from Forest Soil
Curr Microbiol. 2024 Feb 19;81(4):99. doi: 10.1007/s00284-024-03629-5.ABSTRACTA novel Gram-stain-positive, oval-shaped, and non-flagellated bacterial strain YIM S02556T was isolated from forest soil in Xiongbi Town, Shizong County, Qujing City, Yunnan Province, southwestern China. The strain exhibited high pairwise 16 S rRNA gene sequence similarity with Psychromicrobium lacuslunae (97.3%) and Psychromicrobium silvestre (96.3%). Strain YIM S02556T exhibited an average nucleotide identity (ANI) of 72.5% with P. lacuslunae IHBB 11,108T and 72.8% ANI with P. silvestre AK 20-18T. The digital DNA-DNA hybridization (dDDH) value ...
Source: Current Microbiology - February 19, 2024 Category: Microbiology Authors: Ling Zhu Yu-Chen Wang Le-Le Li Xian-Min Huang Man Zhu Xiu Gao Jian Cai Source Type: research
Green-Synthesized Silver Nanoparticles in the Prevention of Multidrug-Resistant Proteus mirabilis Infection and Incrustation of Urinary Catheters BioAgNPs Against P. mirabilis Infection
This study aimed to assess the activity of AgNPs biosynthesized by Fusarium oxysporum (bio-AgNPs) against multidrug-resistant uropathogenic Proteus mirabilis, and to assess the antibacterial activity of catheters coated with bio-AgNPs. Broth microdilution and time-kill kinetics assays were used to determine the antibacterial activity of bio-AgNPs. Catheters were coated with two (2C) and three (3C) bio-AgNPs layers using polydopamine as crosslinker. Catheters were challenged with urine inoculated with P. mirabilis to assess the anti-incrustation activity. MIC was found to be 62.5 µmol l-1, causing total loss of viability a...
Source: Current Microbiology - February 19, 2024 Category: Microbiology Authors: Gustavo Issamu Asai Saikawa Gustavo Henrique Migliorini Guidone Sandriele Aparecida Noriler Guilherme Fonseca Reis Admilton Gon çalves de Oliveira Gerson Nakazato Sergio Paulo Dejato Rocha Source Type: research
Peptoniphilus genitalis sp. nov. and Mobiluncus massiliensis sp. nov.: Novel Bacteria Isolated from the Vaginal Microbiome
Curr Microbiol. 2024 Feb 19;81(4):97. doi: 10.1007/s00284-023-03584-7.ABSTRACTThe strains Marseille-Q7072T (= CSUR Q7072T = CECT 30604 T) and Marseille-Q7826T (= CSUR Q7826T = CECT 30727 T) were isolated from vaginal samples. As MALDI-TOF mass spectrometry failed to identify them, their genomes were directly sequenced to determine their taxogenomic identities. Both strains are anaerobic without any oxidase and catalase activity. C16:0 is the most abundant fatty acid for both strains. Strain Marseille-Q7072T is non-spore-forming, non-motile, Gram-stain-positive, and coccus-shaped, while strain Marseille-Q7826T is non-spore-...
Source: Current Microbiology - February 19, 2024 Category: Microbiology Authors: Linda Abou Chacra Marion Bonnet M égane Heredia Gabriel Haddad Nicholas Armstrong St éphane Alibar Florence Bretelle Florence Fenollar Source Type: research
Divergent Roles of Escherichia Coli Encoded Lon Protease in Imparting Resistance to Uncouplers of Oxidative Phosphorylation: Roles of marA, rob, soxS and acrB
This study addresses the effects of uncouplers and the roles of genes involved during bacterial growth and phenotypic antibiotic resistance. Strong uncouplers are often used to treat wastewater, and these results shed light on the possible mechanisms by which bacteria respond to uncouplers. Also, the rampant usage of some uncouplers to treat wastewater may lead to the development of antibiotic resistance.PMID:38372817 | DOI:10.1007/s00284-024-03632-w (Source: Current Microbiology)
Source: Current Microbiology - February 19, 2024 Category: Microbiology Authors: Taru Verma Santhi Sanil Nandini Varsha Singh Abinaya Raghavan Harshita Annappa Chetana Bhaskarla Ashim Kumar Dubey Dipankar Nandi Source Type: research
Structural Characterization and Functional Studies of Exopolysaccharide by Native Lacticaseibacillus rhamnosus P14 Isolated from the Moroccan Region
Curr Microbiol. 2024 Feb 19;81(4):96. doi: 10.1007/s00284-024-03611-1.ABSTRACTExopolysaccharides (EPS) are natural polymers synthesized by several microorganisms, including lactic acid bacteria (LAB). They are characterized by a great structural diversity, which gives them interesting biological and pharmacological properties. This work investigates the physicochemical and biological characterization of a new exopolysaccharide (EPS) produced by a wild Lacticaseibacillus rhamnosus P14. The functional groups, chemical bonds, and thermal and morphological properties of the purified EPS-P14 were determined using Fourier Transf...
Source: Current Microbiology - February 19, 2024 Category: Microbiology Authors: Akhtach Sihame Tabia Zakaria Mabrouk El Khalil Belkhou Rajae Source Type: research
Rapid Decline of SARS-CoV-2 Viral Load in Single vs. Double-Dose (Short-Interval & lt;6 Weeks) ChAdOx nCoV-19 Vaccinated Health-Care Workers
Curr Microbiol. 2024 Feb 14;81(4):95. doi: 10.1007/s00284-023-03603-7.ABSTRACTThe present work was carried out during the emergence of Delta Variant of Concern (VoC) and aimed to study the change in SARS CoV-2 viral load in Covishield vaccinated asymptomatic/mildly symptomatic health-care workers (HCWs) to find out the optimum isolation period. The SARS CoV-2 viral load was carried out in sequential samples of 55 eligible HCWs which included unvaccinated (UnV; n = 11), single-dose vaccinated (SDV, n = 20) and double-dose vaccinated [DDV, n = 24; short-interval (<6 weeks)] subjects. The mean load of envelope (E) gene on ...
Source: Current Microbiology - February 14, 2024 Category: Microbiology Authors: Arnab Ghosh Poonam Kanta Manisha Ramola Ritin Mohindra Kapil Goyal Roop Kishore Vikas Suri P V M Lakshmi Chanderkanta Chauhan Manisha Sharma Partha Rakshit Kalaiarasan Ponnusamy Tanzin Dikid Mini P Singh Source Type: research
Rapid Decline of SARS-CoV-2 Viral Load in Single vs. Double-Dose (Short-Interval & lt;6 Weeks) ChAdOx nCoV-19 Vaccinated Health-Care Workers
Curr Microbiol. 2024 Feb 14;81(4):95. doi: 10.1007/s00284-023-03603-7.ABSTRACTThe present work was carried out during the emergence of Delta Variant of Concern (VoC) and aimed to study the change in SARS CoV-2 viral load in Covishield vaccinated asymptomatic/mildly symptomatic health-care workers (HCWs) to find out the optimum isolation period. The SARS CoV-2 viral load was carried out in sequential samples of 55 eligible HCWs which included unvaccinated (UnV; n = 11), single-dose vaccinated (SDV, n = 20) and double-dose vaccinated [DDV, n = 24; short-interval (<6 weeks)] subjects. The mean load of envelope (E) gene on ...
Source: Current Microbiology - February 14, 2024 Category: Microbiology Authors: Arnab Ghosh Poonam Kanta Manisha Ramola Ritin Mohindra Kapil Goyal Roop Kishore Vikas Suri P V M Lakshmi Chanderkanta Chauhan Manisha Sharma Partha Rakshit Kalaiarasan Ponnusamy Tanzin Dikid Mini P Singh Source Type: research
Rapid Decline of SARS-CoV-2 Viral Load in Single vs. Double-Dose (Short-Interval & lt;6 Weeks) ChAdOx nCoV-19 Vaccinated Health-Care Workers
Curr Microbiol. 2024 Feb 14;81(4):95. doi: 10.1007/s00284-023-03603-7.ABSTRACTThe present work was carried out during the emergence of Delta Variant of Concern (VoC) and aimed to study the change in SARS CoV-2 viral load in Covishield vaccinated asymptomatic/mildly symptomatic health-care workers (HCWs) to find out the optimum isolation period. The SARS CoV-2 viral load was carried out in sequential samples of 55 eligible HCWs which included unvaccinated (UnV; n = 11), single-dose vaccinated (SDV, n = 20) and double-dose vaccinated [DDV, n = 24; short-interval (<6 weeks)] subjects. The mean load of envelope (E) gene on ...
Source: Current Microbiology - February 14, 2024 Category: Microbiology Authors: Arnab Ghosh Poonam Kanta Manisha Ramola Ritin Mohindra Kapil Goyal Roop Kishore Vikas Suri P V M Lakshmi Chanderkanta Chauhan Manisha Sharma Partha Rakshit Kalaiarasan Ponnusamy Tanzin Dikid Mini P Singh Source Type: research
Rapid Decline of SARS-CoV-2 Viral Load in Single vs. Double-Dose (Short-Interval & lt;6 Weeks) ChAdOx nCoV-19 Vaccinated Health-Care Workers
Curr Microbiol. 2024 Feb 14;81(4):95. doi: 10.1007/s00284-023-03603-7.ABSTRACTThe present work was carried out during the emergence of Delta Variant of Concern (VoC) and aimed to study the change in SARS CoV-2 viral load in Covishield vaccinated asymptomatic/mildly symptomatic health-care workers (HCWs) to find out the optimum isolation period. The SARS CoV-2 viral load was carried out in sequential samples of 55 eligible HCWs which included unvaccinated (UnV; n = 11), single-dose vaccinated (SDV, n = 20) and double-dose vaccinated [DDV, n = 24; short-interval (<6 weeks)] subjects. The mean load of envelope (E) gene on ...
Source: Current Microbiology - February 14, 2024 Category: Microbiology Authors: Arnab Ghosh Poonam Kanta Manisha Ramola Ritin Mohindra Kapil Goyal Roop Kishore Vikas Suri P V M Lakshmi Chanderkanta Chauhan Manisha Sharma Partha Rakshit Kalaiarasan Ponnusamy Tanzin Dikid Mini P Singh Source Type: research
What's in my Pot? Six Colletotrichum Species Causing Anthracnose in Brazilian Pecan Orchards
This study used morphological and molecular approaches to identify the Colletotrichum species causing anthracnose in pecan plantations in Southern Brazil. The isolates obtained from pecan fruits with anthracnose symptoms were grouped through quantitative morphological characteristics into three distinct morphotypes. Molecular analysis of nuclear genes allowed the identification of six species of Colletotrichum causing anthracnose in pecan: C. nymphaeae, C. fioriniae, C. gloeosporioides, C. siamense, C. kahawae, and C. karsti. Three of these species are reported for the first time as causal agents of anthracnose in pecan. T...
Source: Current Microbiology - February 10, 2024 Category: Microbiology Authors: Tales Poletto Yohan Fritsche Vin ícius Spolaor Fantinel Marlove F átima Brião Muniz Ricardo Harakava Valdir Marcos Stefenon Source Type: research
Forskolin Enhances Antitumor Effect of Oncolytic Measles Virus by Promoting Rab27a Dependent Vesicular Transport System
Curr Microbiol. 2024 Feb 9;81(4):93. doi: 10.1007/s00284-024-03613-z.ABSTRACTThe measles vaccine virus strain (MV-Edm) serves as a potential platform for the development of effective oncolytic vectors. Nevertheless, despite promising pre-clinical data, our comprehension of the factors influencing the efficacy of MV-Edm infection and intratumoral spread, as well as the interactions between oncolytic viruses and specific chemotherapeutics associated with viral infection, remains limited. Therefore, we investigated the potency of Forskolin in enhancing the antitumor effect of oncolytic MV-Edm by promoting the Rab27a-dependent...
Source: Current Microbiology - February 9, 2024 Category: Microbiology Authors: Mao Xia Yangbin Wang Yongquan Xia Jiawei Zeng Source Type: research
Actinomycetospora lemnae sp. nov., A Novel Actinobacterium Isolated from Lemna aequinoctialis Able to Enhance Duckweed Growth
In this study, we aimed to report a novel actinobacterium species and explore its ability to enhance duckweed growth. Strain DW7H6T was isolated from duckweed, Lemna aequinoctialis. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that the strain was most closely related to Actinomycetospora straminea IY07-55T (99.0%), Actinomycetospora chibensis TT04-21T (98.9%), Actinomycetospora lutea TT00-04T (98.8%) and Actinomycetospora callitridis CAP 335T (98.4%). Chemotaxonomic and morphological characteristics of strain DW7H6T were consistent with members of the genus Actinomycetospora, while average nucleotide ...
Source: Current Microbiology - February 5, 2024 Category: Microbiology Authors: Yuparat Saimee Waranya Butdee Chanita Boonmak Kannika Duangmal Source Type: research
