The MSMEG_1586 of M. smegmatis Is a Penicillin-Interactive Enzyme That Can Potentially Hydrolyse Aztreonam and Cephalosporins
Curr Microbiol. 2023 Dec 2;81(1):26. doi: 10.1007/s00284-023-03545-0.ABSTRACTMycobacteria are intrinsically resistant to beta-lactams as they possess several putative penicillin-interactive enzymes (PIEs), some of those are with dual-activity, namely DD-carboxypeptidase and beta-lactamase. Here, with help of molecular approaches, we elucidated the nature of one such putative PIE, MSMEG_1586, in Mycobacterium smegmatis. The in vivo expression of the membrane-bound form of MSMEG_1586 enhanced the beta-lactam resistance of a beta-lactamase deleted host E. coli strain (AM1OC), particularly for aztreonam (eight-fold) and cephal...
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Aditya Prasad Panda Satya Deo Pandey Diamond Jain Anindya S Ghosh Source Type: research
Direct Detection of Fluoroquinolone Resistance in Sputum Samples from Tuberculosis Patients by High Resolution Melt Curve Analysis
In conclusion, HRM is a rapid, cost-effective (INR 150/USD 1.83) and closed-tube method for direct detection of FLQ resistance in sputum samples including direct smear-negative samples.PMID:38041739 | DOI:10.1007/s00284-023-03519-2 (Source: Current Microbiology)
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Rakesh Kumar Gupta Divya Anthwal Manpreet Bhalla Jaya Sivaswami Tyagi Sangeeta Choudhary Sagarika Haldar Source Type: research
The MSMEG_1586 of M. smegmatis Is a Penicillin-Interactive Enzyme That Can Potentially Hydrolyse Aztreonam and Cephalosporins
Curr Microbiol. 2023 Dec 2;81(1):26. doi: 10.1007/s00284-023-03545-0.ABSTRACTMycobacteria are intrinsically resistant to beta-lactams as they possess several putative penicillin-interactive enzymes (PIEs), some of those are with dual-activity, namely DD-carboxypeptidase and beta-lactamase. Here, with help of molecular approaches, we elucidated the nature of one such putative PIE, MSMEG_1586, in Mycobacterium smegmatis. The in vivo expression of the membrane-bound form of MSMEG_1586 enhanced the beta-lactam resistance of a beta-lactamase deleted host E. coli strain (AM1OC), particularly for aztreonam (eight-fold) and cephal...
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Aditya Prasad Panda Satya Deo Pandey Diamond Jain Anindya S Ghosh Source Type: research
Direct Detection of Fluoroquinolone Resistance in Sputum Samples from Tuberculosis Patients by High Resolution Melt Curve Analysis
In conclusion, HRM is a rapid, cost-effective (INR 150/USD 1.83) and closed-tube method for direct detection of FLQ resistance in sputum samples including direct smear-negative samples.PMID:38041739 | DOI:10.1007/s00284-023-03519-2 (Source: Current Microbiology)
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Rakesh Kumar Gupta Divya Anthwal Manpreet Bhalla Jaya Sivaswami Tyagi Sangeeta Choudhary Sagarika Haldar Source Type: research
The MSMEG_1586 of M. smegmatis Is a Penicillin-Interactive Enzyme That Can Potentially Hydrolyse Aztreonam and Cephalosporins
Curr Microbiol. 2023 Dec 2;81(1):26. doi: 10.1007/s00284-023-03545-0.ABSTRACTMycobacteria are intrinsically resistant to beta-lactams as they possess several putative penicillin-interactive enzymes (PIEs), some of those are with dual-activity, namely DD-carboxypeptidase and beta-lactamase. Here, with help of molecular approaches, we elucidated the nature of one such putative PIE, MSMEG_1586, in Mycobacterium smegmatis. The in vivo expression of the membrane-bound form of MSMEG_1586 enhanced the beta-lactam resistance of a beta-lactamase deleted host E. coli strain (AM1OC), particularly for aztreonam (eight-fold) and cephal...
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Aditya Prasad Panda Satya Deo Pandey Diamond Jain Anindya S Ghosh Source Type: research
Construction and Immunogenicity Evaluation of Recombinant Bacillus subtilis Expressing HA1 Protein of H9N2 Avian Influenza Virus
This study was the first to use an oral recombinant B. subtilis-HA1 vaccine candidate, and it provides theoretical data and technical reference for the creation of a new live vector vaccine against H9N2 AIV.PMID:38040977 | DOI:10.1007/s00284-023-03548-x (Source: Current Microbiology)
Source: Current Microbiology - December 1, 2023 Category: Microbiology Authors: Zixuan Li Chong Peng Lijun Chen Peng Wang Fangkun Wang Source Type: research
Sensitivities of a Rapid Test Versus an ELISA Kit for Detecting Anti-SARS-CoV-2 IgM/IgG in Sera from an Egyptian Cohort
This study aimed to compare diagnostic sensitivities of a rapid test (Rt) and an ELISA kit for detecting anti-SARS-CoV-2 IgM/IgG in virus-RT-PCR-positive (VPP) and virus-RT-PCR-unchecked (VPU) subjects in an Egyptian cohort during the first wave of SARS-CoV-2 infection. The results revealed higher sensitivity of the Rt for detecting IgM/IgG in the VPP subjects. Both the Rt and ELISA showed identical sensitivities for IgM detection in the VPU subjects. The ELISA was more sensitive for detecting IgG in the VPU subjects. Generally, within both the VPP and the VPU groups, Rt was more sensitive for detecting IgM/IgG among the s...
Source: Current Microbiology - November 30, 2023 Category: Microbiology Authors: Mahmoud Mohamed Bahgat Mohamed Hassan Nasraa Rola Nadeem Khaled Amer Wael A Hassan Fadya M ELGarhy Salem Reda Akinyemi M Fasemore Konrad U F örstner Dina Nadeem Abd-Elshafy Source Type: research
Sensitivities of a Rapid Test Versus an ELISA Kit for Detecting Anti-SARS-CoV-2 IgM/IgG in Sera from an Egyptian Cohort
This study aimed to compare diagnostic sensitivities of a rapid test (Rt) and an ELISA kit for detecting anti-SARS-CoV-2 IgM/IgG in virus-RT-PCR-positive (VPP) and virus-RT-PCR-unchecked (VPU) subjects in an Egyptian cohort during the first wave of SARS-CoV-2 infection. The results revealed higher sensitivity of the Rt for detecting IgM/IgG in the VPP subjects. Both the Rt and ELISA showed identical sensitivities for IgM detection in the VPU subjects. The ELISA was more sensitive for detecting IgG in the VPU subjects. Generally, within both the VPP and the VPU groups, Rt was more sensitive for detecting IgM/IgG among the s...
Source: Current Microbiology - November 30, 2023 Category: Microbiology Authors: Mahmoud Mohamed Bahgat Mohamed Hassan Nasraa Rola Nadeem Khaled Amer Wael A Hassan Fadya M ELGarhy Salem Reda Akinyemi M Fasemore Konrad U F örstner Dina Nadeem Abd-Elshafy Source Type: research
Synergistic Effect of Thymol-Ciprofloxacin Combination on Planktonic Cells and Biofilm of Pseudomonas aeruginosa
This study explores the synergistic effects of Thymol in combination with Ciprofloxacin, Amikacin and Colistin against planktonic cells and biofilm of P. aeruginosa. Thymol in combination with Ciprofloxacin yields the fractional inhibitory concentration index values 0.156 and 0.375 in P. aeruginosa strains, GC14 and ATCC 9027, respectively, highlighting a robust synergistic effect on both the planktonic and biofilm of P. aeruginosa. The results showed that Thymol (512 μg/mL) and Ciprofloxacin (0.125 μg/mL) were the most effective combination with 95 and 93.5% total biofilm inhibition in GC14 and PA27, respectively, compa...
Source: Current Microbiology - November 29, 2023 Category: Microbiology Authors: Devi Jayakumar Minsa Mini Praveen Kumar Parvathi Vaikkathillam Aparna Mohan Sajeeb Khan Source Type: research
Synergistic Effect of Thymol-Ciprofloxacin Combination on Planktonic Cells and Biofilm of Pseudomonas aeruginosa
This study explores the synergistic effects of Thymol in combination with Ciprofloxacin, Amikacin and Colistin against planktonic cells and biofilm of P. aeruginosa. Thymol in combination with Ciprofloxacin yields the fractional inhibitory concentration index values 0.156 and 0.375 in P. aeruginosa strains, GC14 and ATCC 9027, respectively, highlighting a robust synergistic effect on both the planktonic and biofilm of P. aeruginosa. The results showed that Thymol (512 μg/mL) and Ciprofloxacin (0.125 μg/mL) were the most effective combination with 95 and 93.5% total biofilm inhibition in GC14 and PA27, respectively, compa...
Source: Current Microbiology - November 29, 2023 Category: Microbiology Authors: Devi Jayakumar Minsa Mini Praveen Kumar Parvathi Vaikkathillam Aparna Mohan Sajeeb Khan Source Type: research
Taxonomic and Predictive Functional Profile of Hydrocarbonoclastic Bacterial Consortia Developed at Three Different Temperatures
This study found that temperature had a stronger influence on the composition and function of the bacterial community than crude oil concentration.PMID:38017305 | DOI:10.1007/s00284-023-03529-0 (Source: Current Microbiology)
Source: Current Microbiology - November 28, 2023 Category: Microbiology Authors: Ningombam A Singha Roselin Neihsial Lhinglamkim Kipgen Waniabha J Lyngdoh Jopthiaw Nongdhar Bobby Chettri Prabhakar Singh Arvind K Singh Source Type: research
Two-Omics Probe on the Potential of Pseudomonas sp. GDMCC 1.1703 Under Phenol Stress
Curr Microbiol. 2023 Nov 28;81(1):21. doi: 10.1007/s00284-023-03534-3.ABSTRACTPseudomonas sp. harbors genetic diversity and readily adapts to environmental challenges, conferring upon it the ability to remediate. It is important to genetically determine the effects of bacterial application. The two-omics integration approach may shed more light on Pseudomonas isolates, filling the knowledge gap between genetic potential and dynamic function. In the present study, a strain from the Xi River was isolated using benzene-selective enrichment medium and phylogenetically identified as Pseudomonas sp. GDMCC 1.1703 by 16S rRNA gene...
Source: Current Microbiology - November 27, 2023 Category: Microbiology Authors: Hongfei Zhu Jiashuai Yu Yuting Fu Xiaoshuang Mao Haimei Yang Source Type: research
Two-Omics Probe on the Potential of Pseudomonas sp. GDMCC 1.1703 Under Phenol Stress
Curr Microbiol. 2023 Nov 28;81(1):21. doi: 10.1007/s00284-023-03534-3.ABSTRACTPseudomonas sp. harbors genetic diversity and readily adapts to environmental challenges, conferring upon it the ability to remediate. It is important to genetically determine the effects of bacterial application. The two-omics integration approach may shed more light on Pseudomonas isolates, filling the knowledge gap between genetic potential and dynamic function. In the present study, a strain from the Xi River was isolated using benzene-selective enrichment medium and phylogenetically identified as Pseudomonas sp. GDMCC 1.1703 by 16S rRNA gene...
Source: Current Microbiology - November 27, 2023 Category: Microbiology Authors: Hongfei Zhu Jiashuai Yu Yuting Fu Xiaoshuang Mao Haimei Yang Source Type: research
Stenotrophomonas maltophilia Belonging to Novel Sequence Types ST473 and ST474 in Wild Birds Inhabiting the Brazilian Amazonia
Curr Microbiol. 2023 Nov 26;81(1):20. doi: 10.1007/s00284-023-03532-5.ABSTRACTStenotrophomonas maltophilia is an opportunistic human pathogen associated with nosocomial and community-acquired infections. We have conducted a microbiological and genomic surveillance study of broad-spectrum cephalosporin- and carbapenem-resistant Gram-negative bacteria colonizing wild birds inhabiting the Brazilian Amazonia. Strikingly, two S. maltophilia strains (SM79 and SM115) were identified in Plain-throated antwren (Isleria hauxwelli) passerines affected by Amazonian fragmentation and degradation. Noteworthy, SM79 and SM115 strains belo...
Source: Current Microbiology - November 26, 2023 Category: Microbiology Authors: Gislaine Dalazen F ábio Parra Sellera Danny Fuentes-Castillo Elder Sano Herrison Fontana Brenda Cardoso Fernanda Esposito Luis F ábio Silveira Eliana Reiko Matushima Nilton Lincopan Source Type: research
Microbial Diversity in African Foods and Beverages: A Systematic Assessment
This article provides a comprehensive and in-depth examination of the microbial diversity inherent in African food and beverages, with a particular emphasis on fermented products. It identifies and characterizes the dominant microorganisms, including both prokaryotes and yeasts, prevalent in these foods, and furthermore, critically analyzes the health benefits of these microbial strains, especially their probiotic properties, which could potentially improve digestion and contribute to human health. Notably, it underscores the vital role these microorganisms play in bolstering food security across Africa by enhancing and pr...
Source: Current Microbiology - November 26, 2023 Category: Microbiology Authors: Soumya Ghosh Charn é Bornman Maryam Meskini Mehri Joghataei Source Type: research
