Biochemical Characterization of Thermostable Acrylamide Amidohydrolase from Aspergillus fumigatus with Potential Activity for Acrylamide Degradation in Various Food Products
Curr Microbiol. 2023 Dec 5;81(1):30. doi: 10.1007/s00284-023-03544-1.ABSTRACTAcrylamide is the major by-product of the Maillard reactions in foods with the overheating processes of L-asparagine-rich foods with reducing sugars that usually allied with neurotoxicity and carcinogenicity. Several approaches have been used to prevent the formation of acrylamide, however, degrading the already formed acrylamide in foods remains unequivocal. Acrylamide hydrolyzing enzyme "amidohydrolase" is one of the most promising enzymes for acrylamide degradation in foods. So, amidohydrolase "amidase" from thermotolerant Aspergillus fumigatus...
Source: Current Microbiology - December 5, 2023 Category: Microbiology Authors: Ashraf S A El-Sayed Hala N Elghamry Marwa A Yassin Source Type: research
Direct Detection of Fluoroquinolone Resistance in Sputum Samples from Tuberculosis Patients by High Resolution Melt Curve Analysis
In conclusion, HRM is a rapid, cost-effective (INR 150/USD 1.83) and closed-tube method for direct detection of FLQ resistance in sputum samples including direct smear-negative samples.PMID:38041739 | DOI:10.1007/s00284-023-03519-2 (Source: Current Microbiology)
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Rakesh Kumar Gupta Divya Anthwal Manpreet Bhalla Jaya Sivaswami Tyagi Sangeeta Choudhary Sagarika Haldar Source Type: research
The MSMEG_1586 of M. smegmatis Is a Penicillin-Interactive Enzyme That Can Potentially Hydrolyse Aztreonam and Cephalosporins
Curr Microbiol. 2023 Dec 2;81(1):26. doi: 10.1007/s00284-023-03545-0.ABSTRACTMycobacteria are intrinsically resistant to beta-lactams as they possess several putative penicillin-interactive enzymes (PIEs), some of those are with dual-activity, namely DD-carboxypeptidase and beta-lactamase. Here, with help of molecular approaches, we elucidated the nature of one such putative PIE, MSMEG_1586, in Mycobacterium smegmatis. The in vivo expression of the membrane-bound form of MSMEG_1586 enhanced the beta-lactam resistance of a beta-lactamase deleted host E. coli strain (AM1OC), particularly for aztreonam (eight-fold) and cephal...
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Aditya Prasad Panda Satya Deo Pandey Diamond Jain Anindya S Ghosh Source Type: research
Direct Detection of Fluoroquinolone Resistance in Sputum Samples from Tuberculosis Patients by High Resolution Melt Curve Analysis
In conclusion, HRM is a rapid, cost-effective (INR 150/USD 1.83) and closed-tube method for direct detection of FLQ resistance in sputum samples including direct smear-negative samples.PMID:38041739 | DOI:10.1007/s00284-023-03519-2 (Source: Current Microbiology)
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Rakesh Kumar Gupta Divya Anthwal Manpreet Bhalla Jaya Sivaswami Tyagi Sangeeta Choudhary Sagarika Haldar Source Type: research
The MSMEG_1586 of M. smegmatis Is a Penicillin-Interactive Enzyme That Can Potentially Hydrolyse Aztreonam and Cephalosporins
Curr Microbiol. 2023 Dec 2;81(1):26. doi: 10.1007/s00284-023-03545-0.ABSTRACTMycobacteria are intrinsically resistant to beta-lactams as they possess several putative penicillin-interactive enzymes (PIEs), some of those are with dual-activity, namely DD-carboxypeptidase and beta-lactamase. Here, with help of molecular approaches, we elucidated the nature of one such putative PIE, MSMEG_1586, in Mycobacterium smegmatis. The in vivo expression of the membrane-bound form of MSMEG_1586 enhanced the beta-lactam resistance of a beta-lactamase deleted host E. coli strain (AM1OC), particularly for aztreonam (eight-fold) and cephal...
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Aditya Prasad Panda Satya Deo Pandey Diamond Jain Anindya S Ghosh Source Type: research
Direct Detection of Fluoroquinolone Resistance in Sputum Samples from Tuberculosis Patients by High Resolution Melt Curve Analysis
In conclusion, HRM is a rapid, cost-effective (INR 150/USD 1.83) and closed-tube method for direct detection of FLQ resistance in sputum samples including direct smear-negative samples.PMID:38041739 | DOI:10.1007/s00284-023-03519-2 (Source: Current Microbiology)
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Rakesh Kumar Gupta Divya Anthwal Manpreet Bhalla Jaya Sivaswami Tyagi Sangeeta Choudhary Sagarika Haldar Source Type: research
The MSMEG_1586 of M. smegmatis Is a Penicillin-Interactive Enzyme That Can Potentially Hydrolyse Aztreonam and Cephalosporins
Curr Microbiol. 2023 Dec 2;81(1):26. doi: 10.1007/s00284-023-03545-0.ABSTRACTMycobacteria are intrinsically resistant to beta-lactams as they possess several putative penicillin-interactive enzymes (PIEs), some of those are with dual-activity, namely DD-carboxypeptidase and beta-lactamase. Here, with help of molecular approaches, we elucidated the nature of one such putative PIE, MSMEG_1586, in Mycobacterium smegmatis. The in vivo expression of the membrane-bound form of MSMEG_1586 enhanced the beta-lactam resistance of a beta-lactamase deleted host E. coli strain (AM1OC), particularly for aztreonam (eight-fold) and cephal...
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Aditya Prasad Panda Satya Deo Pandey Diamond Jain Anindya S Ghosh Source Type: research
Direct Detection of Fluoroquinolone Resistance in Sputum Samples from Tuberculosis Patients by High Resolution Melt Curve Analysis
In conclusion, HRM is a rapid, cost-effective (INR 150/USD 1.83) and closed-tube method for direct detection of FLQ resistance in sputum samples including direct smear-negative samples.PMID:38041739 | DOI:10.1007/s00284-023-03519-2 (Source: Current Microbiology)
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Rakesh Kumar Gupta Divya Anthwal Manpreet Bhalla Jaya Sivaswami Tyagi Sangeeta Choudhary Sagarika Haldar Source Type: research
The MSMEG_1586 of M. smegmatis Is a Penicillin-Interactive Enzyme That Can Potentially Hydrolyse Aztreonam and Cephalosporins
Curr Microbiol. 2023 Dec 2;81(1):26. doi: 10.1007/s00284-023-03545-0.ABSTRACTMycobacteria are intrinsically resistant to beta-lactams as they possess several putative penicillin-interactive enzymes (PIEs), some of those are with dual-activity, namely DD-carboxypeptidase and beta-lactamase. Here, with help of molecular approaches, we elucidated the nature of one such putative PIE, MSMEG_1586, in Mycobacterium smegmatis. The in vivo expression of the membrane-bound form of MSMEG_1586 enhanced the beta-lactam resistance of a beta-lactamase deleted host E. coli strain (AM1OC), particularly for aztreonam (eight-fold) and cephal...
Source: Current Microbiology - December 2, 2023 Category: Microbiology Authors: Aditya Prasad Panda Satya Deo Pandey Diamond Jain Anindya S Ghosh Source Type: research
Construction and Immunogenicity Evaluation of Recombinant Bacillus subtilis Expressing HA1 Protein of H9N2 Avian Influenza Virus
This study was the first to use an oral recombinant B. subtilis-HA1 vaccine candidate, and it provides theoretical data and technical reference for the creation of a new live vector vaccine against H9N2 AIV.PMID:38040977 | DOI:10.1007/s00284-023-03548-x (Source: Current Microbiology)
Source: Current Microbiology - December 1, 2023 Category: Microbiology Authors: Zixuan Li Chong Peng Lijun Chen Peng Wang Fangkun Wang Source Type: research
Sensitivities of a Rapid Test Versus an ELISA Kit for Detecting Anti-SARS-CoV-2 IgM/IgG in Sera from an Egyptian Cohort
This study aimed to compare diagnostic sensitivities of a rapid test (Rt) and an ELISA kit for detecting anti-SARS-CoV-2 IgM/IgG in virus-RT-PCR-positive (VPP) and virus-RT-PCR-unchecked (VPU) subjects in an Egyptian cohort during the first wave of SARS-CoV-2 infection. The results revealed higher sensitivity of the Rt for detecting IgM/IgG in the VPP subjects. Both the Rt and ELISA showed identical sensitivities for IgM detection in the VPU subjects. The ELISA was more sensitive for detecting IgG in the VPU subjects. Generally, within both the VPP and the VPU groups, Rt was more sensitive for detecting IgM/IgG among the s...
Source: Current Microbiology - November 30, 2023 Category: Microbiology Authors: Mahmoud Mohamed Bahgat Mohamed Hassan Nasraa Rola Nadeem Khaled Amer Wael A Hassan Fadya M ELGarhy Salem Reda Akinyemi M Fasemore Konrad U F örstner Dina Nadeem Abd-Elshafy Source Type: research
Sensitivities of a Rapid Test Versus an ELISA Kit for Detecting Anti-SARS-CoV-2 IgM/IgG in Sera from an Egyptian Cohort
This study aimed to compare diagnostic sensitivities of a rapid test (Rt) and an ELISA kit for detecting anti-SARS-CoV-2 IgM/IgG in virus-RT-PCR-positive (VPP) and virus-RT-PCR-unchecked (VPU) subjects in an Egyptian cohort during the first wave of SARS-CoV-2 infection. The results revealed higher sensitivity of the Rt for detecting IgM/IgG in the VPP subjects. Both the Rt and ELISA showed identical sensitivities for IgM detection in the VPU subjects. The ELISA was more sensitive for detecting IgG in the VPU subjects. Generally, within both the VPP and the VPU groups, Rt was more sensitive for detecting IgM/IgG among the s...
Source: Current Microbiology - November 30, 2023 Category: Microbiology Authors: Mahmoud Mohamed Bahgat Mohamed Hassan Nasraa Rola Nadeem Khaled Amer Wael A Hassan Fadya M ELGarhy Salem Reda Akinyemi M Fasemore Konrad U F örstner Dina Nadeem Abd-Elshafy Source Type: research
Synergistic Effect of Thymol-Ciprofloxacin Combination on Planktonic Cells and Biofilm of Pseudomonas aeruginosa
This study explores the synergistic effects of Thymol in combination with Ciprofloxacin, Amikacin and Colistin against planktonic cells and biofilm of P. aeruginosa. Thymol in combination with Ciprofloxacin yields the fractional inhibitory concentration index values 0.156 and 0.375 in P. aeruginosa strains, GC14 and ATCC 9027, respectively, highlighting a robust synergistic effect on both the planktonic and biofilm of P. aeruginosa. The results showed that Thymol (512 μg/mL) and Ciprofloxacin (0.125 μg/mL) were the most effective combination with 95 and 93.5% total biofilm inhibition in GC14 and PA27, respectively, compa...
Source: Current Microbiology - November 29, 2023 Category: Microbiology Authors: Devi Jayakumar Minsa Mini Praveen Kumar Parvathi Vaikkathillam Aparna Mohan Sajeeb Khan Source Type: research
Synergistic Effect of Thymol-Ciprofloxacin Combination on Planktonic Cells and Biofilm of Pseudomonas aeruginosa
This study explores the synergistic effects of Thymol in combination with Ciprofloxacin, Amikacin and Colistin against planktonic cells and biofilm of P. aeruginosa. Thymol in combination with Ciprofloxacin yields the fractional inhibitory concentration index values 0.156 and 0.375 in P. aeruginosa strains, GC14 and ATCC 9027, respectively, highlighting a robust synergistic effect on both the planktonic and biofilm of P. aeruginosa. The results showed that Thymol (512 μg/mL) and Ciprofloxacin (0.125 μg/mL) were the most effective combination with 95 and 93.5% total biofilm inhibition in GC14 and PA27, respectively, compa...
Source: Current Microbiology - November 29, 2023 Category: Microbiology Authors: Devi Jayakumar Minsa Mini Praveen Kumar Parvathi Vaikkathillam Aparna Mohan Sajeeb Khan Source Type: research
Taxonomic and Predictive Functional Profile of Hydrocarbonoclastic Bacterial Consortia Developed at Three Different Temperatures
This study found that temperature had a stronger influence on the composition and function of the bacterial community than crude oil concentration.PMID:38017305 | DOI:10.1007/s00284-023-03529-0 (Source: Current Microbiology)
Source: Current Microbiology - November 28, 2023 Category: Microbiology Authors: Ningombam A Singha Roselin Neihsial Lhinglamkim Kipgen Waniabha J Lyngdoh Jopthiaw Nongdhar Bobby Chettri Prabhakar Singh Arvind K Singh Source Type: research
