Synthesis of a tetralone derivative of ampicillin to control ampicillin-resistant Staphylococcusaureus
Biochem Biophys Res Commun. 2024 Apr 20;714:149974. doi: 10.1016/j.bbrc.2024.149974. Online ahead of print.ABSTRACTDue to the rapid emergence of antibiotic resistant new bacterial strains and new infections, there is an urgent need for novel or newly modified and efficient alternatives of treatment. However, conventional antibiotics are still used in therapeutic settings but their efficacy is uncertain due to the rapid evolution of drug resistance. In the present study, we have synthesized a new derivative of conventional antibiotic ampicillin using SN2-type substitution reaction. NMR and mass analysis of the newly synthes...
Source: Biochemical and Biophysical Research communications - April 25, 2024 Category: Biochemistry Authors: Sourav Chakraborty Piyush Baindara Suresh K Mondal Dinata Roy Santi M Mandal Source Type: research
A dual-channel electrochemical biosensor enables concurrent detection of pathogens and antibiotic resistance
Biosens Bioelectron. 2024 Apr 20;257:116314. doi: 10.1016/j.bios.2024.116314. Online ahead of print.ABSTRACTDiarrheagenic E. coli infections, commonly treated with β-lactam antibiotics, contribute to antibiotic resistance - a pressing public health concern. Rapid monitoring of pathogen antibiotic resistance is vital to combat antimicrobial spread. Current bacterial diagnosis methods identify pathogens or determine antibiotic resistance separately, necessitating multiple assays. There is an urgent need for tools that simultaneously identify infectious agents and their antibiotic resistance at the point of care (POC). We de...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Dharanivasan Gunasekaran Irina Rostovsky David Taussig Talya Bar-Am Yariv Wine Neta Sal-Man Sefi Vernick Source Type: research
A comprehensive synthetic library of poly-N-acetyl glucosamines enabled vaccine against lethal challenges of Staphylococcus aureus
Nat Commun. 2024 Apr 24;15(1):3420. doi: 10.1038/s41467-024-47457-4.ABSTRACTPoly-β-(1-6)-N-acetylglucosamine (PNAG) is an important vaccine target, expressed on many pathogens. A critical hurdle in developing PNAG based vaccine is that the impacts of the number and the position of free amine vs N-acetylation on its antigenicity are not well understood. In this work, a divergent strategy is developed to synthesize a comprehensive library of 32 PNAG pentasaccharides. This library enables the identification of PNAG sequences with specific patterns of free amines as epitopes for vaccines against Staphylococcus aureus (S. aure...
Source: Cancer Control - April 24, 2024 Category: Cancer & Oncology Authors: Zibin Tan Weizhun Yang Nicholas A O'Brien Xingling Pan Sherif Ramadan Terence Marsh Neal Hammer Colette Cywes-Bentley Mariana Vinacur Gerald B Pier Jeffrey C Gildersleeve Xuefei Huang Source Type: research
Culture-free detection of β-lactamase-Producing bacteria in urinary tract infections using a paper sensor
Biosens Bioelectron. 2024 Apr 22;257:116300. doi: 10.1016/j.bios.2024.116300. Online ahead of print.ABSTRACTDeveloping simple, inexpensive, fast, sensitive, and specific probes for antibiotic-resistant bacteria is crucial for the management of urinary tract infections (UTIs). We here propose a paper-based sensor for the rapid detection of β-lactamase-producing bacteria in the urine samples of UTI patients. By conjugating a strongly electronegative group -N+(CH3)3 with the core structures of cephalosporin and carbapenem antibiotics, two visual probes were achieved to respectively target the extended-spectrum/AmpC β-lactam...
Source: Biosensors and Bioelectronics - April 24, 2024 Category: Biotechnology Authors: Guangming Liu Wenshuai Li Siya Li Jia Xu Xinsheng Wang Hua Xu Dingbin Liu Hongmei Gao Source Type: research
Genes, Vol. 15, Pages 532: Resistome, Virulome, and Clonal Variation in Methicillin-Resistant Staphylococcus aureus (MRSA) in Healthy Swine Populations: A Cross-Sectional Study
Patricia Poeta
This cross-sectional study investigates the methicillin-resistant Staphylococcus aureus (MRSA): its prevalence, antimicrobial resistance, and molecular characteristics in healthy swine populations in central Portugal. A total of 213 samples were collected from pigs on twelve farms, and MRSA prevalence was assessed using selective agar plates and confirmed via molecular methods. Antimicrobial susceptibility testing and whole genome sequencing (WGS) were performed to characterize resistance profiles and genetic determinants. Among the 107 MRSA-positive samples (83.1% prevalence), fattening pigs and breed...
Source: Genes - April 24, 2024 Category: Genetics & Stem Cells Authors: Vanessa Silva Adriana Silva Raquel Barbero Mario Romero Rosa del Campo Manuela Cani ça Rui Cordeiro Gilberto Igrejas Patricia Poeta Tags: Article Source Type: research
Molecules, Vol. 29, Pages 1939: Fractionation of Carlina acaulis L. Root Methanolic Extract as a Promising Path towards New Formulations against Bacillus cereus and Methicillin-Resistant Staphylococcus aureus
In this study, the fractionation of methanol extract from the root was conducted. Four fractions (A, B, C, and D) were obtained and tested against a range of bacteria and fungi. The results showed promising antibacterial activity, especially against Bacillus cereus, where the minimal inhibitory concentration (MIC) was determined to be equal to 0.08 mg/mL and 0.16 mg/mL for heptane (fraction B) and ethyl acetate (fraction C), respectively. In the case of the methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300 strain, the same fractions yielded higher MIC values (2.5 and 5.0 mg/mL, respectively). This was accompani...
Source: Molecules - April 24, 2024 Category: Chemistry Authors: Sylwia Wnorowska Agnieszka Grzegorczyk Jacek Kurzepa Filippo Maggi Maciej Strzemski Tags: Article Source Type: research
Evaluation of omadacycline regimens for community-acquired bacterial pneumonia patients infected with < em > Staphylococcus Aureus < /em > by pharmacokinetic/pharmacodynamic analysis
J Chemother. 2024 Apr 22:1-8. doi: 10.1080/1120009X.2024.2343963. Online ahead of print.ABSTRACTOmadacycline is an FDA-approved agent for community-acquired bacterial pneumonia (CABP). The purpose of this study is to evaluate the effectiveness of omadacycline for treating CABP patients infected with Staphylococcus aureus, including Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin-Susceptible Staphylococcus aureus (MSSA), using pharmacokinetic/pharmacodynamic (PK/PD) analysis. Monte Carlo simulations (MCSs) were performed by utilizing omadacycline pharmacokinetic (PK) parameters, minimum inhibitory concent...
Source: Journal of Chemotherapy - April 23, 2024 Category: Cancer & Oncology Authors: Gaoqi Xu Xin Liu Jiaqi Wang Yuqing Mei Dihong Yang Chaoneng He Like Zhong Junfeng Zhu Haiying Ding Luo Fang Source Type: research
Application of Mono and Trinuclear Cyclometalated Iridium (III) Complexes in Differential Bacterial Imaging and Antimicrobial Photodynamic therapy
Chemistry. 2024 Apr 23:e202400646. doi: 10.1002/chem.202400646. Online ahead of print.ABSTRACTThe application of transition metal complexes for antimicrobial photodynamic therapy (PDT) has emerged as an attractive alternative in mitigating a broad range of bacterial pathogens, including multidrug-resistant pathogens. In view of their photostability, long excited-state lifetimes, and tunable emission properties, transition metal complexes also contribute as bioimaging agents. In the present work, we designed mono and trinuclear cyclometalated iridium(III) complexes to explore their imaging application and antibacterial pote...
Source: Chemistry - April 23, 2024 Category: Chemistry Authors: Bishnu Das Prakash Biswas Amirul Islam Mallick Parna Gupta Source Type: research
Evaluation of omadacycline regimens for community-acquired bacterial pneumonia patients infected with < em > Staphylococcus Aureus < /em > by pharmacokinetic/pharmacodynamic analysis
J Chemother. 2024 Apr 22:1-8. doi: 10.1080/1120009X.2024.2343963. Online ahead of print.ABSTRACTOmadacycline is an FDA-approved agent for community-acquired bacterial pneumonia (CABP). The purpose of this study is to evaluate the effectiveness of omadacycline for treating CABP patients infected with Staphylococcus aureus, including Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin-Susceptible Staphylococcus aureus (MSSA), using pharmacokinetic/pharmacodynamic (PK/PD) analysis. Monte Carlo simulations (MCSs) were performed by utilizing omadacycline pharmacokinetic (PK) parameters, minimum inhibitory concent...
Source: Journal of Chemotherapy - April 23, 2024 Category: Cancer & Oncology Authors: Gaoqi Xu Xin Liu Jiaqi Wang Yuqing Mei Dihong Yang Chaoneng He Like Zhong Junfeng Zhu Haiying Ding Luo Fang Source Type: research
Application of Mono and Trinuclear Cyclometalated Iridium (III) Complexes in Differential Bacterial Imaging and Antimicrobial Photodynamic therapy
Chemistry. 2024 Apr 23:e202400646. doi: 10.1002/chem.202400646. Online ahead of print.ABSTRACTThe application of transition metal complexes for antimicrobial photodynamic therapy (PDT) has emerged as an attractive alternative in mitigating a broad range of bacterial pathogens, including multidrug-resistant pathogens. In view of their photostability, long excited-state lifetimes, and tunable emission properties, transition metal complexes also contribute as bioimaging agents. In the present work, we designed mono and trinuclear cyclometalated iridium(III) complexes to explore their imaging application and antibacterial pote...
Source: Chemistry - April 23, 2024 Category: Chemistry Authors: Bishnu Das Prakash Biswas Amirul Islam Mallick Parna Gupta Source Type: research
Proteomic profiling of antibiotic ‐resistant Escherichia coli GW‐AmxH19 isolated from hospital wastewater treated with physical plasma
AbstractMicroorganisms which are resistant to antibiotics are a global threat to the health of humans and animals. Wastewater treatment plants are known hotspots for the dissemination of antibiotic resistances. Therefore, novel methods for the inactivation of pathogens, and in particular antibiotic-resistant microorganisms (ARM), are of increasing interest. An especially promising method could be a water treatment by physical plasma which provides charged particles, electric fields, UV-radiation, and reactive species. The latter are foremost responsible for the antimicrobial properties of plasma. Thus, with plasma it might...
Source: Proteomics - April 22, 2024 Category: Biochemistry Authors: Veronika Hahn,
Daniela Z ühlke,
Hauke Winter,
Annchristin Landskron,
Jörg Bernhardt,
Susanne Sievers,
Michael Schmidt,
Thomas von Woedtke,
Katharina Riedel,
Juergen F. Kolb Tags: RESEARCH ARTICLE Source Type: research
Synthesis and preclinical evaluation of novel 18F-vancomycin-based tracers for the detection of bacterial infections using positron emission tomography
ConclusionHere we present the synthesis and first evaluation of the vancomycin-based PET tracers [18F]BODIPY-FL-vancomycin and [18F]PQ-VE1-vancomycin for image-guided detection of Gram-positive bacteria. Our study paves the way towards real-time bacteria-targeted diagnosis of soft tissue and implant-associated infections that are oftentimes caused by Gram-positive bacteria, even after prophylactic treatment with vancomycin. (Source: European Journal of Nuclear Medicine and Molecular Imaging)
Source: European Journal of Nuclear Medicine and Molecular Imaging - April 22, 2024 Category: Nuclear Medicine Source Type: research
Unveilling genetic profiles and correlations of biofilm-associated genes, quorum sensing, and antibiotic resistance in Staphylococcus aureus isolated from a Malaysian Teaching Hospital
ConclusionsThe high prevalence of biofilm and slime production among MSSA and MRSA isolates correlates well with the detection of a high prevalence of biofilm-associated genes andagr quorum sensing system. A significant association ofagrI gene was found with cefoxitin, oxacillin, and fluoroquinolone resistance. A more focused approach targeting biofilm-associated and quorum sensing genes is important in developing new surveillance and treatment strategies againstS. aureus biofilm infection. (Source: European Journal of Medical Research)
Source: European Journal of Medical Research - April 22, 2024 Category: Research Source Type: research
Synthesis and preclinical evaluation of novel < sup > 18 < /sup > F-vancomycin-based tracers for the detection of bacterial infections using positron emission tomography
CONCLUSION: Here we present the synthesis and first evaluation of the vancomycin-based PET tracers [18F]BODIPY-FL-vancomycin and [18F]PQ-VE1-vancomycin for image-guided detection of Gram-positive bacteria. Our study paves the way towards real-time bacteria-targeted diagnosis of soft tissue and implant-associated infections that are oftentimes caused by Gram-positive bacteria, even after prophylactic treatment with vancomycin.PMID:38644432 | DOI:10.1007/s00259-024-06717-7 (Source: Molecular Medicine)
Source: Molecular Medicine - April 21, 2024 Category: Molecular Biology Authors: G B Spoelstra S N Blok L Reali Nazario L Noord Y Fu N A Simeth F F A IJpma M van Oosten J M van Dijl B L Feringa W Szymanski P H Elsinga Source Type: research
Chemo-photothermal therapy of chitosan/gold nanorod clusters for antibacterial treatment against the infection of planktonic and biofilm MRSA
In this study, we developed chemo-photothermal therapy (chemo-PTT) based on near-infrared (NIR)-irradiated chitosan/gold nanorod (GNR) clusters as anti-methicillin-resistant Staphylococcus aureus (MRSA) agents. The nanocomposites exhibited an average size of 223 nm with a surface charge of 36 mV. These plasmonic nanocomposites demonstrated on-demand and rapid hyperthermal action under NIR. The combined effect of positive charge and PTT by NIR-irradiated nanocomposites resulted in a remarkable inhibition rate of 96 % against planktonic MRSA, indicating a synergistic activity compared to chitosan nanoparticles or GNR alone. ...
Source: International Journal of Biological Macromolecules - April 20, 2024 Category: Biochemistry Authors: G R Nirmal Zih-Chan Lin Tai-Sheng Chiu Ahmed Alalaiwe Chia-Chih Liao Jia-You Fang Source Type: research
