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Total 393 results found since Jan 2013.

Procyanidin B2 induces porcine skeletal slow-twitch myofiber gene expression by AMP-activated protein kinase signaling pathway
In this study, our aim is to investigate the effect of dimer procyanidin B2 [epicatechin-(4β-8)-epicatechin] (PB2) on porcine skeletal myofiber gene expression in vitro. Our data showed PB2 promoted the protein expression of slow myosin heavy chain (MyHC) in porcine myotubes, concomitant with the increases in mRNA levels of MyHC I, MyHC IIa and Tnni1. We also found PB2 activated AMPK signaling in porcine myotubes. NRF1 and CaMKKβ that are two important upstream factors of AMPK, and Sirt1 and PGC-1α that are two major downstream factors of AMPK, were also up-regulated by PB2. The mechanism study showed the effect of PB2 ...
Source: Animal Biotechnology - June 1, 2021 Category: Biotechnology Authors: Meng Xu Xiaoling Chen Zhiqing Huang Daiwen Chen Bing Yu Jun He Hong Chen Jie Yu Yuheng Luo Ping Zheng Source Type: research

Leucine regulates porcine muscle fiber type transformation via adiponectin signaling pathway
Anim Biotechnol. 2021 Mar 11:1-9. doi: 10.1080/10495398.2021.1892709. Online ahead of print.ABSTRACTLeucine can promote slow-twitch muscle fibers formation, and this effect may be mediated by AMPK signaling pathway. In addition, adiponectin (AdipoQ) plays an important role in regulation of muscle fiber type transformation. AdipoQ is located in the upstream of AMPK and its secretion can be regulated by leucine. Therefore, the aim of this study was to explore whether leucine affects muscle fiber type transformation through AdipoQ signaling pathway. Our data showed that 4 mM leucine significantly increased protein expression ...
Source: Animal Biotechnology - March 11, 2021 Category: Biotechnology Authors: Lu Xiang Zhiqing Huang Xiaoling Chen Gang Jia Guangmang Liu Hua Zhao Source Type: research

Hepatitis B virus DNA polymerase displays an anti-apoptotic effect by interacting with elongation factor 1-alpha2 in hepatoma cells.
In this study, we aimed at investigating the impact of HBV Pol on host cellular processes, mainly apoptosis, and the underlying mechanisms. We showed a marked reduction in apoptotic rates in the HBV Pol-expressed HepG2 cells compared to controls. Moreover, a series of assays-i.e. yeast two-hybrid, GST pull-down, co-immunoprecipitation, and confocal laser scanning microscopy-identified the host factor eEF1A2 to be associated with HBV Pol. Furthermore, knockdown of the eEF1A2 gene by siRNA abrogated the HBV Pol-mediated anti-apoptotic effect with apoptosis induced by endoplasmatic reticulum (ER) stress inducer thapsigargin (...
Source: Journal of Microbiology and Biotechnology - October 23, 2020 Category: Biotechnology Authors: Niu X, Nong S, Gong J, Zhang X, Tang H, Zhou T, Li W Tags: J Microbiol Biotechnol Source Type: research

Mucus-penetrating PEGylated polysuccinimide-based nanocarrier for intravaginal delivery of siRNA battling sexually transmitted infections.
Abstract Intravaginal delivery of siRNA for prevention of sexually transmitted infections faces obstacles such as the acidic environment and vaginal mucus barrier. To achieve effective protection and delivery of siRNA, we developed a polysuccinimide (PSI)-based nanocarrier (PSI-PEG-API-PMA, PPAP) by conjugating methoxy polyethylene glycol amine (Me-PEG-NH2, Mw 5000), 1-(3-aminopropyl)imidazole (API), and 1-pyrenemethylamine hydrochloride (PMA) to PSI. PPAP demonstrated a spherical self-assembled nanostructure before and after encapsulation of a model siRNA. Variable electrostatic interaction between API and siRNA ...
Source: Colloids and Surfaces - July 28, 2020 Category: Biotechnology Authors: Currie S, Kim S, Gu X, Ren X, Lin F, Liu S, Yang C, Kim JH, Liu S Tags: Colloids Surf B Biointerfaces Source Type: research

Study and Evaluation of the Potential of Lipid Nanocarriers for Transdermal Delivery of siRNA.
In this study, we used 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or lipofectamine to prepare a nanocarrier for delivering siRNA against glyceraldehyde 3-phosphate dehydrogenase (GAPDH); GAPDH expression was then evaluated at the cellular level. In addition, a dermal transport assay was designed and implemented to evaluate the penetration and delivery efficacy of siRNA in pig skin using lipid nanocarriers. The delivery of siRNA with the use of a lipid nanocarrier was significantly better than the delivery of siRNA without it. Thus, Our findings identify lipid nanocarriers as excellent candidates for the transdermal d...
Source: Biotechnology Journal - July 16, 2020 Category: Biotechnology Authors: Lee K, Min D, Choi Y, Kim J, Yoon S, Jang J, Park S, Tanaka M, Cho YW, Koo HJ, Jeon H, Choi J Tags: Biotechnol J Source Type: research

Noncoding RNAs in Medicinal Plants and Their Regulatory Roles in Bioactive Compound Production.
CONCLUSION: So far, scientists have identified thousands of miRNA candidates from over 50 medicinal plant species and 11794 lncRNAs from Salvia miltiorrhiza, Panax ginseng, and Digitalis purpurea. Among them, more than 30 miRNAs and five lncRNAs have been predicted to regulate bioactive compound production. The regulation may achieve through various regulatory modules and pathways, such as the miR397-LAC module, the miR12112-PPO module, the miR156-SPL module, the miR828-MYB module, the miR858-MYB module, and other siRNA and lncRNA regulatory pathways. Further functional analysis of herbal ncRNAs will provide useful informa...
Source: Current Pharmaceutical Biotechnology - May 27, 2020 Category: Biotechnology Authors: Li C, Wang M, Qiu X, Zhou H, Lu S Tags: Curr Pharm Biotechnol Source Type: research

Mechanical loading induces HIF-1 α expression in chondrocytes via YAP.
CONCLUSION: Our results indicated that cyclic mechanical stress promoted HIF-1α stabilization and YAP is involved in mechanical stress induced HIF-1α up-regulation. PMID: 32451801 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - May 24, 2020 Category: Biotechnology Authors: Jing X, Yang X, Zhang W, Wang S, Cui X, Du T, Li T Tags: Biotechnol Lett Source Type: research

Newly synthesized peptide, Ara ‐27, exhibits significant improvement in cell‐penetrating ability compared to conventional peptides
In conclusion, this study describes a novel CPP, Ara‐27, which exhibit significant improvement in intracellular uptake compared to conventional CPPs, without affecting cell viability. Thus, development of Ara‐27 based peptides may lead to imp roved delivery of functional cargo such as small molecules, siRNA, and drugs forin vivo studies.This article is protected by copyright. All rights reserved.
Source: Biotechnology Progress - May 5, 2020 Category: Biotechnology Authors: Sol Min, Kichul Kim, Seockmo Ku, Jeong ‐Yoon Park, Jeongmin Seo, Sangho Roh Tags: RESEARCH ARTICLE Source Type: research

Natural ligand-receptor mediated loading of siRNA in milk derived exosomes
Publication date: Available online 1 May 2020Source: Journal of BiotechnologyAuthor(s): Shruti Shandilya, Payal Rani, Suneel Kumar Onteru, Dheer Singh
Source: Journal of Biotechnology - May 2, 2020 Category: Biotechnology Source Type: research

siRNA-Mediated MrIAG Silencing Induces Sex Reversal in Macrobrachium rosenbergii.
Abstract The insulin-like androgenic gland (IAG) gene is well known in male crustacean, and it is a key regulator in male sexual differentiation and maintaining the male sexual characteristic. The neo-female can be produced by silencing the MrIAG (Macrobrachium rosenbergii Insulin-like Androgenic Gland) in male Macrobrachium rosenbergii. This is the first time to use siRNA approach to silenced MrIAG in male M. rosenbergii. In the current study, the optimal injection dosage to achieve sex reversal is 0.5 μg/g body weight. After MrIAG silencing, the expression level of Dmrt11e, Dmrt99b, MRPINK, Mrr, Sxl1, and Sxl2...
Source: Marine Biotechnology - April 26, 2020 Category: Biotechnology Authors: Tan K, Zhou M, Jiang H, Jiang D, Li Y, Wang W Tags: Mar Biotechnol (NY) Source Type: research

SiRNA-directed self-assembled quantum dot biosensor for simultaneous detection of multiple microRNAs at the single-particle level
Publication date: Available online 26 March 2020Source: Biosensors and BioelectronicsAuthor(s): Fei Ma, Su Jiang, Chun-yang Zhang
Source: Biosensors and Bioelectronics - March 27, 2020 Category: Biotechnology Source Type: research