Development of an antibody control system using phage display
Publication date: Available online 23 May 2019Source: Journal of BiotechnologyAuthor(s): Zsolt Keresztessy, Edina Erdos, Lilla Ozgyin, János Kádas, József Horváth, Gábor Zahuczky, Balint L. BalintAbstractWhile chromatin immunoprecipitation has become a widely-used method in the field of transcription regulation studies, serious limitations connected to the complexity and relatively little standardization of the method serve as obstacles for its use in clinical research. In this paper we introduce a method for developing bacteriophage-based controls for the better standardization of the ch...
Source: Journal of Biotechnology - May 24, 2019 Category: Biotechnology Source Type: research

One-pot, three-step cascade synthesis of D-danshensu using engineered Escherichia coli whole cells
Publication date: Available online 21 May 2019Source: Journal of BiotechnologyAuthor(s): Tianzhen Xiong, Pu jia, Jing Jiang, Yajun Bai, Taiping Fan, Xiaohui Zheng, Yujie CaiAbstractD-danshensu (D-DSS), extracted from the plant Salvia miltiorrhiza (Danshen), is widely used to treat cardiovascular and cerebrovascular diseases. Here we engineered Escherichia coli strains to produce D-DSS from catechol, pyruvate and ammonia by one-pot biotransformation. Tyrosin-phenol lyase (TPL), L-amino acid deaminase (aadL), D-lactate dehydrogenase (ldhD) and glucose dehydrogenase (gdh) genes were overexpressed in Escherichia coli strain. F...
Source: Journal of Biotechnology - May 22, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 20 June 2019Source: Journal of Biotechnology, Volume 299Author(s): (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - May 18, 2019 Category: Biotechnology Source Type: research

TP53, EGFR and PIK3CA gene variations observed as prominent biomarkers in breast and lung cancer by plasma cell-free DNA genomic testing
Publication date: Available online 14 May 2019Source: Journal of BiotechnologyAuthor(s): Savli Hakan, Sertdemir Nilufer, Aydin Duygu, Dursun Bilge, Kurtas Omer, Reka Seda, Sunnetci-Akkoyunlu Deniz, Eren-Keskin Seda, Uygun Kazim, Ozden Ercan, Isik Ulas, Cabuk Devrim, Demir Gulhan, Guzdolu Eda, Gokbayrak Merve, Aksu Maksut Gorkem, CAnturk Nuh Zafer, Cine NaciABSTRACTUse of plasma cell-free DNA genomic testing, also know as liquid biopsy, reveals information for early detection and monitoring of solid tumors. Our study reports the analysis of 113 lung and 18 breast cancer patients using commercially available platforms. Lung ...
Source: Journal of Biotechnology - May 16, 2019 Category: Biotechnology Source Type: research

Synergistic improvement of PQQ-dependent D-sorbitol dehydrogenase activity from Gluconobacter oxydans for the biosynthesis of miglitol precursor 6-(N-hydroxyethyl)-amino-6-deoxy-α-L-sorbofuranose
In this study, two subunits sldA and sldB of mSLDH were introduced into G. oxydans ZJB-605, and the specific enzyme activity of mSLDH towards NHEG was enhanced by 2.15-fold. However, the endogenous PQQ level was dramatically reduced in the recombinant strain and became a bottleneck to support the holo-enzyme activity. A combined supplementation of four amino acids (Glu, Ile, Ser, Arg) involved in biosynthesis of PQQ in conventional media effectively increased extracellular accumulation of PQQ by 1.49-fold, which further enhanced mSLDH activity by 1.33-fold. The synergic improvement of mSLDH activity provided in this study ...
Source: Journal of Biotechnology - May 16, 2019 Category: Biotechnology Source Type: research

Extracellular transglycosylase and glyceraldehyde-3-phosphate dehydrogenase attributed to the anti-staphylococcal activity of Lactobacillus plantarum USM8613
Publication date: Available online 13 May 2019Source: Journal of BiotechnologyAuthor(s): Jia Sin Ong, Todd D. Taylor, Chyn Boon Wong, Boon Yin Khoo, Sreenivasan Sasidharan, Sy Bing Choi, Hiroshi Ohno, Min Tze LiongAbstractIncreasing levels of antibiotic resistance in pathogens, including Staphylococcus aureus, remains a serious problem for public health, leading to the need for better alternative antimicrobial strategies. The antimicrobial proteins produced by Lactobacillus plantarum USM8613 attributed to its anti-staphylococcal activity were identified as extracellular transglycosylase and glyceraldehyde-3-phosphate dehyd...
Source: Journal of Biotechnology - May 15, 2019 Category: Biotechnology Source Type: research

Cloning and functional characterization of epidermis-specific promoter MtML1 from Medicago truncatula
Publication date: Available online 11 May 2019Source: Journal of BiotechnologyAuthor(s): Li Gao, Tian Ye, Meng-Ci Chen, Wei Li, Tian-Ge Gao, Hong-Ju Yin, Jin-Lin Zhang, Tanweer Kumar, Lin-Bo Liu, Suo-Min WangAbstractEpidermis-specific promoters are necessary for ectopic expression of specific functional genes such as the cuticle-related genes. Previous studies indicated that both ECERIFERUM 6 (AtCER6) and ARABIDOPSIS THALIANA MERISTEM L1 LAYER (ATML1) promoters from Arabidopsis thaliana can drive gene expression specifically in the epidermis of shoot apical meristems (SAMs) and leaves. However, the epidermis-specifi...
Source: Journal of Biotechnology - May 12, 2019 Category: Biotechnology Source Type: research

Simple yet effective: microbial and biotechnological benefits of rumen liquid addition to lignocellulose-degrading biogas plants
This study compares 0 %, 20 % and 40 % rumen liquid in a batch anaerobic digestion approach. Moreover, it determines the biogas- and methane-potentials as well as degradation-speeds of corn straw, co-digested with cattle manure. It inspects microbial communities via marker-gene sequencing, qPCR and RNA-DGGE and draws attention on possible beneficial effects of rumen addition on the biogas-producing community. Bioaugmentation with 20 % and 40 % v/v rumen liquid accelerated methane yields by 5 and 6 days, respectively (i.e. reaching 90% of total methane production). It also enhanced LCB- as well as (hemi)cellulose- and volat...
Source: Journal of Biotechnology - May 12, 2019 Category: Biotechnology Source Type: research

Computational analysis and optimization of carcinoembryonic antigen aptamers and experimental evaluation
In this study, computational methods were used to optimize CEA aptamers. Experimental evaluvation of selected aptamers were conducted through electrochemical impedance spectroscopy. After two and three-dimensional structure modeling, the complexes of twelve reported aptamers against CEA were simulated using the ZDOCK server. Based on docking scores, two aptamer sequences (CSR59 and CSR57.1) were selected and used to create a new library. This ssDNA aptamer library consisting of 91 sequences was created using diverse in silico mutational methods. We obtained seventeen sequences having higher binding scores than reported seq...
Source: Journal of Biotechnology - May 9, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 10 June 2019Source: Journal of Biotechnology, Volume 298Author(s): (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - May 7, 2019 Category: Biotechnology Source Type: research

Optimization of process conditions for mammalian fed-batch cell culture in automated micro-bioreactor system using genetic algorithm
In this study, we have successfully used a simple genetic algorithm as a method of experimental design for optimization of mammalian cell culture processes for two recombinant cell lines, one expressing a monoclonal antibody and one an Fc-fusion protein. Harnessing the automation capability of a robotically driven micro-bioreactor system to execute the genetic algorithm-derived experiments, a set of 14 process parameters was optimized within 132 experiments per cell line (six generations of 22 experiments), showing the feasibility of genetic algorithm approach as an alternative to classical statistical experimental designs...
Source: Journal of Biotechnology - May 6, 2019 Category: Biotechnology Source Type: research

Quantification of peripheral whole blood, cell-free plasma and exosome encapsulated mitochondrial DNA copy numbers in patients with atrial fibrillation
Publication date: Available online 4 May 2019Source: Journal of BiotechnologyAuthor(s): Beáta Soltész, Réka Urbancsek, Ondrej Pös, Orsolya Hajas, Ildikó Noémi Forgács, Edina Szilágyi, Edina Nagy-Baló, Tomas Szemes, Zoltán Csanádi, Bálint NagyAbstractMitochondrial DNA (mtDNA) copy number changes have been associated with various diseases. Several studies showed that mtDNA content in peripheral blood was associated with oxidative stress and cardiovascular disease. Atrial fibrillation (AF) is one of the severe cardiovascular diseases. We aimed t...
Source: Journal of Biotechnology - May 5, 2019 Category: Biotechnology Source Type: research

Screening for preeclampsia in the first trimester of pregnancy in routine clinical practice in Hungary
In conclusion, using maternal characteristics, biophysical parameters, and PAPP-A, an acceptable screening efficacy could be achieved for early-PE during first-trimester screening. Since PlGF did not improve efficacy in our study, we suggest setting new standard curves for PlGF in Eastern European pregnant women, and the evaluation of novel biochemical markers. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - May 4, 2019 Category: Biotechnology Source Type: research

Glucose limitation and glucose uptake rate determines metabolite production and sporulation in high cell density continuous cultures of Bacillus amyloliquefaciens 83
Publication date: Available online 2 May 2019Source: Journal of BiotechnologyAuthor(s): Sergio Andrés Cristiano-Fajardo, Celia Flores, Noemí Flores, Raunel Tinoco-Valencia, Leobardo Serrano-Carreón, Enrique GalindoAbstractBacillus amyloliquefaciens spores have been used as the principal ingredient of biocontrol products. However, during the process of spore production, wild-type strains produce poly-γ-glutamic acid (γ-PGA), an undesirable byproduct that increases broth viscosity and hinders recovery and drying. This work examined the influence of specific glucose uptake rates (qGluc) in glu...
Source: Journal of Biotechnology - May 4, 2019 Category: Biotechnology Source Type: research

Overexpression of tomato SlbHLH22 transcription factor gene enhances fruit sensitivity to exogenous phytohormones and shortens fruit shelf-life
In this study, we isolated the tomato SlbHLH22 and expressing significantly in fruits at early and later stages of development. The SlbHLH22 was overexpressed under control of CaMV 35S promoter. The transgenic tomato fruits exhibited sensitivity to exogenous phytohormone, such as ABA and IAA and ripened fast than WT fruits. However, the fruits remain insensitive to GA. It was observed that the transcript abundance of ABA and IAA related genes were also significantly upregulated in fruits overexpressing SlbHLH22. Compared to WT, the activities of peroxidase, superoxide dismutase, and catalase was lower in the transgenic fru...
Source: Journal of Biotechnology - May 2, 2019 Category: Biotechnology Source Type: research

Non-invasive prenatal testing as a valuable source of population specific allelic frequencies
Publication date: Available online 1 May 2019Source: Journal of BiotechnologyAuthor(s): Budis Jaroslav, Gazdarica Juraj, Radvanszky Jan, Harsanyova Maria, Gazdaricova Iveta, Strieskova Lucia, Frno Richard, Duris Frantisek, Minarik Gabriel, Sekelska Martina, Nagy Balint, Szemes TomasAbstractLow-coverage massively parallel genome sequencing for non-invasive prenatal testing (NIPT) of common aneuploidies is one of the most rapidly adopted and relatively low-cost DNA tests. Since aggregation of reads from a large number of samples allows overcoming the problems of extremely low coverage of individual samples, we describe the p...
Source: Journal of Biotechnology - May 2, 2019 Category: Biotechnology Source Type: research

Copy number variants detection by microarray and multiplex ligation-dependent probe amplification in congenital heart diseases
Publication date: Available online 1 May 2019Source: Journal of BiotechnologyAuthor(s): Orsolya Nagy, Katalin Szakszon, Brigitta Orsolya Biró, Gábor Mogyorósy, Dóra Nagy, Bálint Nagy, István Balogh, Anikó UjfalusiAbstractCongenital heart diseases (CHDs) are the most common birth defects among life births, which could be presented as isolated or syndromic with other congenital malformations. The etiology of CHD largely unknown, genetic and environmental factors contribute to the disease. Recurrent copy number variants (CNVs) have been reported in the pathogenesis of CHD. The ...
Source: Journal of Biotechnology - May 2, 2019 Category: Biotechnology Source Type: research

PEGAylated graphene oxide/superparamagnetic nanocomposite as a high-efficiency loading nanocarrier for controlled delivery of methotrexate
Publication date: 10 June 2019Source: Journal of Biotechnology, Volume 298Author(s): Zahra Abdollahi, Asghar Taheri-Kafrani, Seyed Amir Bahrani, Abolghasem Abbasi KajaniAbstractPolymer-coated nanocarriers play an important role in targeted drug delivery. The use of polymers such as polyethylene glycol increases stability, biocompatibility, and blood circulation time of the drug, and may consequently improve the success of drug delivery. In the present work, a simple approach has been reported for synthesizing polyethylene glycol bis amin (PEGA) functionalized graphene oxide/iron oxide nanocomposite as a remarkable unit for...
Source: Journal of Biotechnology - May 1, 2019 Category: Biotechnology Source Type: research

Effect of aliphatic diamine spacer length on enzymatic performance of myrosinase immobilized on chitosan microsphere and its application for sulforaphene production
Publication date: Available online 28 April 2019Source: Journal of BiotechnologyAuthor(s): Jie Zhang, Chunrong Feng, Xuemei Tan, Peter-Leon Hagedoorn, Changhong Gu, Hui Xu, Xing ZhouAbstractGlucosinolates can be hydrolyzed by the enzyme commonly known as myrosinase (E.C. 3.2.1.147) to a variety of biological compounds. Myrosinase (MYR) has been immobilized through the flexible spacers of different length on cross-linked chitosan resin (CCR). Ethylene diamine, hexamethylenediamine and decamethylene-diamine have been separately used as spacers. The influence of the flexible spacer length on the immobilized MYR (IMYR) propert...
Source: Journal of Biotechnology - April 29, 2019 Category: Biotechnology Source Type: research

Highly efficient asymmetric reduction of 2-octanone in biphasic system by immobilized Acetobacter sp. CCTCC M209061 cells
Publication date: Available online 29 April 2019Source: Journal of BiotechnologyAuthor(s): Xiao-Yang Ou, Xiao-Ling Wu, Fei Peng, Pei Xu, Shi-Yu Zhang, Min-Hua Zong, Wen-Yong LouAbstractHighly efficient asymmetric reduction of 2-octanone to (R)-2-octanol catalyzed by immobilized Acetobacter sp. CCTCC M209061 cells was achieved in a biphasic system. Bioreduction conducted in aqueous single phase buffer was limited due to poor solubility and toxicity towards cells cause by product accumulation. Introduction of [C4MIM]·Ac accelerated the biotransformation process, giving 99% yield,>99% product e.e. and 1.42-fold high...
Source: Journal of Biotechnology - April 29, 2019 Category: Biotechnology Source Type: research

A dynamic model-based preparation of uniformly-13C-labeled internal standards facilitates quantitative metabolomics analysis of Penicillium chrysogenum
In conclusion, the IDMS method can be incorporated into well-established fast sampling and quenching protocols to obtain dynamic quantitative in vivo metabolome data at the timescales of (tens of) seconds and elucidate the underlying regulatory architecture. The case study revealed gross differences between single and repeated pulses, which suggests that single pulse studies have limited value for understanding of metabolic responses in large-scale bioreactors. Instead, intermittent feeding should be favored. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - April 29, 2019 Category: Biotechnology Source Type: research

PITX2 and NEURL1 SNP polymorphisms in Hungarian atrial fibrillation patients determined by quantitative real-time PCR and melting curve analysis
Publication date: Available online 27 April 2019Source: Journal of BiotechnologyAuthor(s): Krisztina Szirák, Beáta Soltész, Orsolya Hajas, Réka Urbancsek, Edina Nagy-Baló, András Penyige, Zoltán Csanádi, Bálint NagyAbstractAtrial fibrillation (AF) is the most common cardiac arrhythmia affecting 1-2% of the general population. Some common variants located in or next to PITX2 and NEURL1 genes are proved to play role in the occurrence of AF. The aim of our study was to investigate whether rs2595104 in the 4q25 chromosome region and rs6584555 SNP in the NEURL1 gene...
Source: Journal of Biotechnology - April 28, 2019 Category: Biotechnology Source Type: research

Classification of different types of estrogen receptor alpha binding sites in MCF-7 cells
Publication date: Available online 27 April 2019Source: Journal of BiotechnologyAuthor(s): Dóra Bojcsuk, Bálint László BálintAbstractEstrogen Receptor alpha (ERα) is a ligand-activated transcription factor and it has a prominent role in both physiological and pathological processes of the reproductive system. ERα has been investigated extensively in breast cancer and the MCF-7 breast-cancer-derived cell line is a widely used model for the study of its behavior. In this paper we provide a systematic catalog of the possible scenarios of binding to more than 80,000 ERα tran...
Source: Journal of Biotechnology - April 28, 2019 Category: Biotechnology Source Type: research

Uncertainty of fetal fraction determination in Non-Invasive Prenatal Screening by highly polymorphic SNPs
Publication date: Available online 26 April 2019Source: Journal of BiotechnologyAuthor(s): Marian Grendár, Dušan Loderer, Zuzana Laučeková, Iveta Švecová, Michaela Hrtánková, Andrea Hornáková, Bálint Nagy, Pavol Žúbor, Zora Lasabová, Ján DankoAbstractFetal fraction and the chromosome representation are the two key quantities used in Non-Invasive Prenatal Screening (NIPS) to determine the aneuploidy status of a fetus. Several methods for fetal fraction determination have been proposed in the literature, including a class of the meth...
Source: Journal of Biotechnology - April 28, 2019 Category: Biotechnology Source Type: research

Ultracentrifugation enrichment protocol followed by total RNA sequencing allows assembly of the complete mitochondrial genome
Publication date: Available online 22 April 2019Source: Journal of BiotechnologyAuthor(s): Lucia Strieskova, Iveta Gazdaricova, Michal Kajsik, Katarina Soltys, Jaroslav Budis, Ondrej Pos, Martina Lickova, Boris Klempa, Tomas SzemesAbstractThe mitochondrial genome is an independent genetic system in each eukaryotic cell outside the nuclear genome. Mitochondrial DNA (mtDNA) appears in high copy number within one cell, unlike nuclear DNA, which exists in two copies. But nevertheless, mtDNA represent only small part of total cellular DNA what causes problematic analysis and identification of relevant mutations. While most rese...
Source: Journal of Biotechnology - April 24, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 20 May 2019Source: Journal of Biotechnology, Volume 297Author(s): (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - April 19, 2019 Category: Biotechnology Source Type: research

Detection of cell-free, exosomal and whole blood mitochondrial DNA copy number in plasma or whole blood of patients with serous epithelial ovarian cancer
Publication date: Available online 16 April 2019Source: Journal of BiotechnologyAuthor(s): Judit Szilvia Keserű, Beáta Soltész, János Lukács, Éva Márton, Melinda Szilágyi-Bónizs, András Penyige, Róbert Póka, Bálint NagyAbstractOvarian tumor is one of the leading causes of cancer among women. Patients are diagnosed at an advanced stage, usually. There is a need for new specific and sensitive biomarkers.Mitochondrial DNA copy number change was observed in various cancers. Our aim was to detect mitochondrial DNA copy number in whole blood (wb...
Source: Journal of Biotechnology - April 18, 2019 Category: Biotechnology Source Type: research

Predictive accuracy of the breast cancer genetic risk model based on eight common genetic variants: The BACkSIDE study
Publication date: Available online 16 April 2019Source: Journal of BiotechnologyAuthor(s): Danková Zuzana, Žúbor Pavol, Grendár Marián, Zelinová Katarína, Jagelková Marianna, Šťastný Igor, Kapinová Andrea, Vargová Daniela, Kasajová Petra, Dvorská Dana, Kalman Michal, Danko Ján, Lasabová ZoraAbstractBreast cancer (BC) development is caused by the interaction of environmental and genetic factors. At least 90 susceptible genetic variants with different population penetration and incidence have been associated with BC. Th...
Source: Journal of Biotechnology - April 18, 2019 Category: Biotechnology Source Type: research

On the critical evaluation and confirmation of germline sequence variants identified using massively parallel sequencing
Publication date: Available online 15 April 2019Source: Journal of BiotechnologyAuthor(s): Zuzana Kubiritova, Marianna Gyuraszova, Emilia Nagyova, Michaela Hyblova, Maria Harsanyova, Jaroslav Budis, Rastislav Hekel, Juraj Gazdarica, Frantisek Duris, Ludevit Kadasi, Tomas Szemes, Jan RadvanszkyAbstractAlthough massively parallel sequencing (MPS) is becoming common practice in both research and routine clinical care, confirmation requirements of identified DNA variants using alternative methods are still topics of debate. When evaluating variants directly from MPS data, different read depth statistics, together with speciali...
Source: Journal of Biotechnology - April 16, 2019 Category: Biotechnology Source Type: research

Inhibitory effect of Bacillus velezensis on biofilm formation by Streptococcus mutans
Publication date: Available online 12 April 2019Source: Journal of BiotechnologyAuthor(s): Yesol Yoo, Dong-Ho Seo, Hyunjin Lee, Eui-Sang Cho, Nho-Eul Song, Tea Gyu Nam, Young-Do Nam, Myung-Ji SeoAbstractStreptococcus mutans plays a key role in the development of dental caries and promotes the formation of oral biofilm produced by glucosyltransferases (GTFs). Bacillus velezensis K68 was isolated from traditional fermented foods and inhibits biofilm formation mediated by S. mutans. Gene amplification results demonstrated that B. velezensis K68 contained genes for the biosynthesis of 1-deoxynojirimycin (1-DNJ), a known GTF ex...
Source: Journal of Biotechnology - April 14, 2019 Category: Biotechnology Source Type: research

PEGAylated graphene oxide/superparamagnetic nanocomposite as a high-efficienc loading nanocarrier for controlled delivery of methotrexate
Publication date: Available online 12 April 2019Source: Journal of BiotechnologyAuthor(s): Zahra Abdollahi, Asghar Taheri-Kafrani, Seyed Amir Bahrani, Abolghasem Abbasi KajaniAbstractPolymer-coated nanocarriers play an important role in targeted drug delivery. The use of polymers such as polyethylene glycol increases stability, biocompatibility, and blood circulation time of the drug, and may consequently improve the success of drug delivery. In the present work, a simple approach has been reported for synthesizing polyethylene glycol bis amin (PEGA) functionalized graphene oxide/iron oxide nanocomposite as a remarkable un...
Source: Journal of Biotechnology - April 14, 2019 Category: Biotechnology Source Type: research

Significance of Liquid Biopsy in Glioblastoma – A Review
Publication date: Available online 12 April 2019Source: Journal of BiotechnologyAuthor(s): Álmos Klekner, László Szivos, József Virga, Péter Árkosy, László Bognár, Zsuzsanna Birkó, Bálint NagyAbstractGlioblastoma (GBM) is the most common and most aggressive primary malignant brain tumor with a 16-24-months overall survival time (OS). Effective management is hindered by intratumoral heterogeneity, a characteristic trait of GBM which results in subpopulations of cells with altered therapeutic responsiveness, different invasiveness and growth potenti...
Source: Journal of Biotechnology - April 13, 2019 Category: Biotechnology Source Type: research

Production, purification and characterization of an elastin-like polypeptide containing the Ile-Lys-Val-Ala-Val (IKVAV) peptide for tissue engineering applications
In conclusion, this functionalized ELP-IKVAV biomaterial shows interesting properties for tissue engineering requiring neurotization.Graphical abstract (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - April 11, 2019 Category: Biotechnology Source Type: research

Target genes for plant productivity improvement
Publication date: Available online 9 April 2019Source: Journal of BiotechnologyAuthor(s): Nowicka BeatryczeAbstractThe use of chemical fertilizers and pesticides, as well as the development of high-yielding varieties enabled substantial increase in crop productivity during the 20th century. However, the increase in yield over the last two decades has been slower. It is thought that further improvement in productivity of the major crop species using traditional cultivation methods is limited. Therefore, the use of genetic engineering seems to be a promising approach. There is ongoing research concerning genes that have an i...
Source: Journal of Biotechnology - April 10, 2019 Category: Biotechnology Source Type: research

Increased fermentative adenosine production by gene-targeted Bacillus subtilis mutation
In this study, guanosine 5′-monophosphate (GMP) synthetase gene (guaA) was inactivated by gene-target manipulation to increase the metabolic flux from inosine 5′-monophosphate (IMP) to adenosine in B. subtilis A509. The resulted mutant M3-3 showed an increased adenosine production from 7.40 to 10.45 g/L, which was further enhanced to a maximum of 14.39 g/L by central composite design. As the synthesis of succinyladenosine monophosphate (sAMP) from IMP catalysed by adenylosuccinate synthetase (encoded by purA gene) is the rate-limiting step in adenosine synthesis, the up-regulated transcription lev...
Source: Journal of Biotechnology - April 10, 2019 Category: Biotechnology Source Type: research

Enhancement of galactose consumption rate in Saccharomyces cerevisiae CEN.PK2-1 by CRISPR Cas9 and adaptive evolution for fermentation of Kappaphycus alvarezii hydrolysate
Publication date: Available online 5 April 2019Source: Journal of BiotechnologyAuthor(s): In Yung Sunwoo, Pailin Sukwong, Deok Yeol Jeong, Soo Rin Kim, Gwi-Teak Jeong, Sung-Koo KimAbstractEthanol ferrmentation of Kappaphycus alvarezii hydrolysates was performed using wild-type (WT) Saccharomyces cerevisiae CEN.PK2-1, hexokinase 2 deleted (Δhxk2) and adapted strain on high galactose concentrations. The WT and Δhxk2 strains produced 8.9 and 14.67 g/L of ethanol with yield coefficient (YEtOH) of 0.20 and 0.33 (g/g), respectively. However, neither the WT nor Δhxk2strain could utilize all of the galacto...
Source: Journal of Biotechnology - April 7, 2019 Category: Biotechnology Source Type: research

Assessment of Fed-batch Cultivation Strategies for an Inducible CHO Cell Line
Publication date: Available online 5 April 2019Source: Journal of BiotechnologyAuthor(s): Kahina Mellahi, Denis Brochu, Michel Gilbert, Michel Perrier, Sven Ansorge, Yves Durocher, Olivier HenryAbstractIn order to maximize cell growth and productivity for an inducible CHO cell line expressing rituximab, various fed-batch culture strategies were investigated. In each case, the performance was evaluated for cultures induced at moderate and high cell density conditions (4 × 106 and 10 × 106 cells/mL) to assess the impact of the timing of induction. We first demonstrate the importance of...
Source: Journal of Biotechnology - April 7, 2019 Category: Biotechnology Source Type: research

Quantitative RT-PCR-based miRNA profiling of blastemal Wilms’ tumors from formalin-fixed paraffin-embedded samples
Publication date: Available online 5 April 2019Source: Journal of BiotechnologyAuthor(s): Gergely Buglyó, Zsófia Magyar, Éva Romicsné Görbe, Rita Bánusz, Monika Csóka, Tamás Micsik, Zsanett Berki, Péter Varga, Zoltán Sápi, Bálint NagyAbstractBlastemal Wilms’ tumors are associated with poor chemo-responsiveness and an adverse prognosis. Our aim was to contribute to the miRNA profiling of the disease, while demonstrating the value of archived formalin-fixed, paraffin-embedded (FFPE) samples as miRNA sources. MiRNA was extracted from tumor...
Source: Journal of Biotechnology - April 7, 2019 Category: Biotechnology Source Type: research

Constructing an efficient salicylate biosynthesis platform by Escherichia coli chromosome integration
In this study, an efficient SA producing strain was constructed by step-by-step chromosome integration. First, the SA biosynthetic module controlled by promoters PT7 or Ptac was integrated into the chromosome of E. coli, generating the basal strain SA producing strains. PT7 performed better than Ptac and led to higher SA titers, the best of which was 233.6 ± 6.6 mg/L. Disrupting pheA/tyrA eliminated accumulation of phenylalanine and tyrosine, improving SA titer to 492.4 ± 9.3 mg/L. Strengthening the upstream pathway by enhancing expression of aroG further improved SA ...
Source: Journal of Biotechnology - April 7, 2019 Category: Biotechnology Source Type: research

Expression of CD24 in plasma, exosome and ovarian tissue samples of serous ovarian cancer patients
Publication date: Available online 5 April 2019Source: Journal of BiotechnologyAuthor(s): Beáta Soltész, János Lukács, Edina Szilágyi, Éva Márton, Melinda Szilágyi Bónizs, András Penyige, Róbert Póka, Bálint NagyAbstractCD24 is a small molecular weight cell-surface protein and an independent marker for poor prognosis in the different type of cancers. We aimed to determine the expression of CD24 in plasma, exosomes and ovarian tissue samples of serous ovarian cancer patients.We collected tissue and blood samples from 21 cases of serou...
Source: Journal of Biotechnology - April 7, 2019 Category: Biotechnology Source Type: research

Circulating epithelial-mesenchymal transition-associated miRNAs are promising biomarkers in ovarian cancer
Publication date: Available online 3 April 2019Source: Journal of BiotechnologyAuthor(s): Éva Márton, János Lukács, András Penyige, Eszter Janka, Lídia Hegedüs, Beáta Soltész, Gábor Méhes, Róbert Póka, Bálint Nagy, Melinda SzilágyiAbstractOvarian cancer is the fifth most common cause of cancer death among women that is mostly due to the difficulty of early diagnosis. Circulating miRNAs proved to be reliable biomarkers in various cancers. We screened 9 miRNAs, which are involved in epithelial-mesenchymal transition, in the...
Source: Journal of Biotechnology - April 4, 2019 Category: Biotechnology Source Type: research

Liquid biopsy for predictive mutational profiling of solid cancer: the pathologist’s perspective
Publication date: Available online 3 April 2019Source: Journal of BiotechnologyAuthor(s): Gábor MéhesAbstractThe pathological diagnostics of cancer - based on the histological features - is today increasingly completed by molecular profiling at variable depth in an almost evident fashion. Predictive information should cover potential therapeutic targets and/or major resistance mechanisms the nature of which is subject of alteration during the course of the treatment. Mutational profiling recently became technically available by the analysis of circulating free DNA obtained following non-invasive peripheral bl...
Source: Journal of Biotechnology - April 4, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 20 April 2019Source: Journal of Biotechnology, Volume 296Author(s): (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - April 4, 2019 Category: Biotechnology Source Type: research

Enhancing the synthesis of latex clearing protein by different cultivation strategies
In this study, we improved the synthesis of the latex clearing protein from Gordonia polyisoprenivorans VH2 (Lcp1VH2), a key enzyme for the initial cleavage of the rubber backbone. Cultivations using a recombinant strain of Escherichia coli were optimized to overcome poor solubility of Lcp1VH2 and improve the production yields. Different cultivation temperatures and agitation rates were evaluated in the process to demonstrate their impact on the solubility of Lcp1VH2. A specific maximum production rate of 28.3 mg Lcp1VH2 g-1 cell dry weight h-1 was obtained at 25 °C and at agitation rates between 2...
Source: Journal of Biotechnology - March 31, 2019 Category: Biotechnology Source Type: research

Comparison of plasma and urinary microRNA-483-5p for the diagnosis of adrenocortical malignancy
Publication date: Available online 30 March 2019Source: Journal of BiotechnologyAuthor(s): Abel Decmann, Irina Bancos, Aakanksha Khanna, Melinda A. Thomas, Péter Turai, Pál Perge, József Zsolt Pintér, Miklós Tóth, Attila Patócs, Peter IgazAbstractIntroductionMinimally invasive circulating microRNAs might be used for the preoperative differentiation of adrenocortical carcinoma (ACC) and adrenocortical adenoma (ACA). So far, the best blood-borne microRNA biomarker of ACC is circulating hsa-miR-483-5p. The expression of urinary hsa-miR-483-5p as a non-invasive marker of maligna...
Source: Journal of Biotechnology - March 31, 2019 Category: Biotechnology Source Type: research

Discovery of three novel sesquiterpene synthases from Streptomyces chartreusis NRRL 3882 and crystal structure of an α-eudesmol synthase
This study describes the discovery of three novel sesquiterpene synthases from Streptomyces chartreusis with preference for the formation of germacradiene-11-ol, α-eudesmol and α-amorphene respectively. The α-eudesmol synthase showed formation of 10-epi-δ-eudesmol and elemol as side products. Eudesmol-isomers are known to have repellent activity, which makes this enzyme a potential catalyst for products for the prevention of mosquito-related disease. The determination of the structure of the apo-enzyme of α-eudesmol synthase from S. chartreusis provides the first structural insights into an eu...
Source: Journal of Biotechnology - March 28, 2019 Category: Biotechnology Source Type: research

Effects of a 15-amino-acid isoform of amyloid- β expressed by silkworm pupae on B6C3-Tg Alzheimer’s disease transgenic mice
In this study, we used silkworm pupae to develop a safer vaccine for Alzheimer’s disease (AD) patients. Aβ15 peptide was fused with the cholera toxin B subunit (CTB) and expressed in silkworm pupae. Then, we tested an oral vaccine with the peptide expressed by silkworm pupae in a transgenic mouse model of AD. The results show that anti-Aβ antibodies were induced, Aβ deposition in the brain decreased, the content of malondialdehyde was lower than in the other group, and memory and cognition of the mice improved. These results suggest that the high-nutrient CTB-Aβ15 silkworm pupa vaccine has ...
Source: Journal of Biotechnology - March 28, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 10 April 2019Source: Journal of Biotechnology, Volume 295Author(s): (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - March 24, 2019 Category: Biotechnology Source Type: research

Monitoring and Control Strategies for Inclusion Body Production in E. coli based on Glycerol Consumption
Publication date: Available online 20 March 2019Source: Journal of BiotechnologyAuthor(s): Christoph Slouka, Julian Kopp, Daniel Strohmer, Julian Kager, Oliver Spadiut, Christoph HerwigAbstractThe Gram-negative bacterium E. coli is the host of choice for the production of a multitude of recombinant proteins in industry. Generally, cultivation is easy, media are cheap and a high product titer can be obtained. However, harsh induction procedures using IPTG as inducer are often referred to cause stress reactions, leading to a phenomenon known as metabolic burden and expression of inclusion bodies. In this contribution, we pre...
Source: Journal of Biotechnology - March 21, 2019 Category: Biotechnology Source Type: research

Identification of miR-146a and miR-196a-2 single nucleotide polymorphisms at patients with high-grade serous ovarian cancer
Publication date: Available online 20 March 2019Source: Journal of BiotechnologyAuthor(s): János Lukács, Beáta Soltész, András Penyige, Bálint Nagy, Róbert PókaAbstractMicroRNAs play an essential role in the regulation of gene expression and tumor development. Single nucleotide polymorphism (SNP) can be observed in miRNAs and could influence gene expression.We aimed to identify miR-146a rs2910164 and miR-196a-2 rs11614913 polymorphisms in ovarian cancer patients and controls.75 patients and 75 controls were involved. DNA was isolated from blood samples. MiR-146a rs291...
Source: Journal of Biotechnology - March 21, 2019 Category: Biotechnology Source Type: research