Preparation of a Reversible soluble-insoluble β-d-Glucosidase with Perfect Stability and Activity
Publication date: Available online 4 January 2019Source: Journal of BiotechnologyAuthor(s): Lu Li, Huaxiao Li, Bin Yan, Shitao YuAbstractRebuilding enzymes with high activities, stability and reusability has been constantly pursued for application of enzymatic
catalysis in wide field. Rebuilding enzymes by chemical approaches, including immobilization and chemical
modification, represents a simple but effective route. Here, a class of highly stable and active reversible soluble-insoluble β-d-Glucosidase (E-CB-ALD 5K) was prepared using mPEG-ALD (methoxy-polyethylene glycol aldehyde, molecular weight: 5k) as a site...
Source: Journal of Biotechnology - January 5, 2019 Category: Biotechnology Source Type: research

Microbial Production of Glycolate from Acetate by Metabolically Engineered Escherichia coli
Publication date: Available online 4 January 2019Source: Journal of BiotechnologyAuthor(s): Wei Li, Jing Chen, Chang-Xia Liu, Qi-Peng Yuan, Zheng-Jun LiAbstractEscherichia coli was metabolically engineered to synthesize glycolate using acetate as the carbon source. The native glyoxylate bypass pathway was reinforced by the overexpression of isocitrate lyase and isocitrate dehydrogenase kinase/phosphatase. Glyoxylate/hydroxypyruvate reductase was overexpressed to convert glyoxylate to glycolate. Meanwhile, side reactions were eliminated by inactivating genes encoding malate synthase, glyoxylate carboligase, and glycolate ox...
Source: Journal of Biotechnology - January 4, 2019 Category: Biotechnology Source Type: research

Overexpression of RCK1 improves acetic acid tolerance in Saccharomyces cerevisiae
Publication date: Available online 4 January 2019Source: Journal of BiotechnologyAuthor(s): Eun Joong Oh, Na Wei, Suryang Kwak, Heejin Kim, Yong-Su JinAbstractMixed sugars derived from lignocellulosic biomass can be converted into biofuels and chemicals by engineered microorganisms, but toxic fermentation inhibitors produced from harsh depolymerization processes of lignocellulosic biomass pose a critical challenge for economic production of biofuels and chemicals. Unlike other fermentation inhibitors generated from sugar degradation, acetic acid is inevitably produced from acetylated hemicellulose, and its concentrations i...
Source: Journal of Biotechnology - January 4, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 20 January 2019Source: Journal of Biotechnology, Volume 290Author(s): (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - January 4, 2019 Category: Biotechnology Source Type: research

Corrigendum to “Whole-genome sequence of purple non-sulfur bacteria, Rhodobacter sphaeroides strain MBTLJ-8 with improved CO2 reduction capacity” [J Biotechnol 288./ (2018) 9-14]
Publication date: 20 January 2019Source: Journal of Biotechnology, Volume 290Author(s): Ju-Yong Park, Bit-Na Kim, Yang-Hoon Kim, Jiho Min (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - January 4, 2019 Category: Biotechnology Source Type: research

Discovery of a new NADPH-dependent aldo-keto reductase from Candida orthopsilosis catalyzing the stereospecific synthesis of (R)-pantolactone by genome mining
In this study, we investigated another synthetic method of (R)-pantothenate through the stereoselective reduction of ketopantoyl lactone (KPL) by aldo-keto reductase (AKR). A series of conjugated polyketone reductases (CPRs) were discovered from GenBank database by genome mining approach. The putative CPR gene from Candida orthopsilosis Co 90-125 (CorCPR) was cloned and functionally expressed in Escherichia coli BL21 (DE3). The optimum pH and temperature of recombinant CorCPR were 6.0-7.0 and 40 ℃, respectively. The Km and vmax toward KPL were1.3 mM and 227.3 μmol/min/mg protein, respectively. The conserved seq...
Source: Journal of Biotechnology - December 27, 2018 Category: Biotechnology Source Type: research

A novel anti-cancer L19-interleukin-12 fusion protein with an optimized peptide linker efficiently localizes in vivo at the site of tumors
Publication date: Available online 23 December 2018Source: Journal of BiotechnologyAuthor(s): Tiziano Ongaro, Mattia Matasci, Samuele Cazzamalli, Baptiste Gouyou, Roberto De Luca, Dario Neri, Alessandra VillaAbstractAntibody-cytokine fusion proteins are a class of biopharmaceuticals, with the potential to modulate the activity of the immune system at the site of disease. The molecular format used to connect antibody moiety and cytokine payload can have a profound influence on biological activity and pharmacokinetic properties. The optimization of fusion protein format is particularly challenging for heterodimeric cytokines...
Source: Journal of Biotechnology - December 24, 2018 Category: Biotechnology Source Type: research

Bacterial cellulose as a support for yeast immobilization – correlation between carrier properties and process efficiency
Publication date: Available online 20 December 2018Source: Journal of BiotechnologyAuthor(s): Anna Żywicka, Anna Banach, Adam F. Junka, Radosław Drozd, Karol FijałkowskiAbstractThe aim of the current study was to analyze physicochemical properties of bacterial cellulose (BC) produced by Komagataeibacter xylinus for various periods of time in stationary conditions with regard to its potential as a carrier for yeast (Saccharomyces cerevisiae and Yarrowia lipolytica) immobilization, and subsequently to correlate the relationship between these properties and the efficiency of the immobilization process. Physicochemical prop...
Source: Journal of Biotechnology - December 20, 2018 Category: Biotechnology Source Type: research

Metabolic engineering of Corynebacterium glutamicum for the fermentative production of halogenated tryptophan
In this study, fermentative production of the halogenated amino acid 7-chloro-L-tryptophan from sugars, ammonium and chloride salts was achieved. This required metabolic engineering of an L-tryptophan producing C. glutamicum strain for expression of the genes coding for FAD-dependent halogenase RebH and NADH-dependent flavin reductase RebF from Lechevalieria aerocolonigenes. Chlorination of L-tryptophan to 7-chloro-L-tryptophan by recombinant C. glutamicum was improved by optimizing the RBS of rebH. Metabolic engineering enabled production of 7-chloro-L-tryptophan and L-tryptophan from the alternative carbon sources arabin...
Source: Journal of Biotechnology - December 20, 2018 Category: Biotechnology Source Type: research

One-Pot Synthesis of a 3-Thiazolidine through Combination of an Asinger-type Multi-Component-Condensation Reaction with an Enzymatic Imine Reduction
Publication date: Available online 20 December 2018Source: Journal of BiotechnologyAuthor(s): Nadine Zumbrägel, Harald GrögerAbstractAddressing the combination of heterocyclic chemistry and biocatalysis, a one-pot synthesis of a 3-thiazolidine by means of an initial Asinger-type multi-component-condensation reaction under formation of a 3-thiazoline and a subsequent enzymatic reduction of this cyclic imine is reported. The one-pot process is conducted in a sequential mode and without the need for a spatial compartmentalization of the single reaction steps. For the biocatalytic reduction an imine reductase was use...
Source: Journal of Biotechnology - December 20, 2018 Category: Biotechnology Source Type: research

Targeted delivery of calreticulin to ED-A fibronectin leads to tumor-growth retardation
We report the design and characterization of novel fusion proteins, consisting of the F8 antibody and of murine calreticulin (Calr). The F8 antibody recognizes the alternatively-spliced ED-A domain of fibronectin, an extracellular matrix component found in most tumor types, while calreticulin has previously been described as an “eat-me” signal for dendritic cells and phagocytes. Four fusion proteins, differing in antibody formats and peptide linkers, were produced in mammalian cells, purified to homogeneity and tested in vitro and in vivo. A quantitative biodistribution in F9 tumor-bearing mice revealed that th...
Source: Journal of Biotechnology - December 20, 2018 Category: Biotechnology Source Type: research

Substrate specificity of novel GH16 endo-β-(1→3)-galactanases acting on linear and branched β-(1→3)-galactooligosaccharides
In this study, three novel endo-β-(1→3)-galactanases were identified and the substrate specificity was further studied using well-defined galactan oligomers. Linear and branched β-(1→3)-linked galactans, which resemble the carbohydrate core of the arabinogalactan protein, were used for the characterization of endo-β-(1→3)-galactanases. The identified enzymes required at least three consecutive galactose residues for activity. Non-substituted regions were preferred, but substituents in the -2 and +2 and in some cases also -1 and +1 subsites were tolerated to some extent, depending on the branch...
Source: Journal of Biotechnology - December 18, 2018 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 10 January 2019Source: Journal of Biotechnology, Volume 289Author(s): (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - December 18, 2018 Category: Biotechnology Source Type: research

Enantioselective synthesis of enantiopure β-amino alcohols via kinetic resolution and asymmetric reductive amination by a robust transaminase from Mycobacterium vanbaalenii
Publication date: Available online 13 December 2018Source: Journal of BiotechnologyAuthor(s): Jian-Dong Zhang, Jian-Wei Zhao, Li-Li Gao, Hong-Hong Chang, Wen-Long Wei, Jian-He XuAbstractChiral β-amino alcohols are very important chiral building block for preparing bioactive compounds for use in pharmaceutical and fine chemical industries. Synthesis of chiral β-amino alcohols by transaminase is big challenging due to the strict substrate specificities and very low activity of the enzyme. In this work, a (R)-selective ω-transaminase (MVTA) from Mycobacterium vanbaalenii was employed as a biocatalyst for the f...
Source: Journal of Biotechnology - December 14, 2018 Category: Biotechnology Source Type: research

Disruption of por1 gene in Candida utilis improves co-production of S-adenosylmethionine and glutathione
Publication date: Available online 13 December 2018Source: Journal of BiotechnologyAuthor(s): Dahui Wang, Dechao Li, Gaochuan Zhang, Chonglong Wang, Gongyuan WeiAbstractThe por1 gene encoding one of the mitochondrial porin channels in C. utilis CCTCC M 209298 was disrupted using a homologous recombination method. The co-production of S-adenosylmethionine (SAM) and glutathione (GSH) in the mutant C. utilis Δpor1 increased by 34.9% and 25.1%, respectively, during batch and fed-batch fermentation, relative to the parental strain. The average oxygen consumption rate, activities of key enzymes involved in SAM and GSH bios...
Source: Journal of Biotechnology - December 14, 2018 Category: Biotechnology Source Type: research

Comparison and implementation of different control strategies for improving production of rHSA using Pichia pastoris
Publication date: Available online 11 December 2018Source: Journal of BiotechnologyAuthor(s): Piyanka, Souhardya Roy, Viki Chopda, James Gomes, Anurag S. RathoreAbstractRecombinant protein production by various host organisms is strictly regulated, which is determined mainly by host cell genetics and maintenance of process variables within desirable limits in a reactor. Since the process dynamics typically exhibit nonlinear interactions amongst the critical process variables, the commonly employed controllers are suboptimal for control of most bioprocesses. In this article, we present the implementation of an augmented con...
Source: Journal of Biotechnology - December 12, 2018 Category: Biotechnology Source Type: research

Highly Efficient Production of Chiral Amines in Batch and Continuous Flow by Immobilized ω-Transaminases on Controlled Porosity Glass Metal-Ion Affinity Carrier
In this study, two stereocomplementary ω-transaminases from Arthrobacter sp. (AsR-ωTA) and Chromobacterium violaceum (Cv-ωTA) were immobilized via iron cation affinity binding onto polymer-coated controlled porosity glass beads (EziGTM). The immobilization procedure was studied with different types of carrier materials and immobilization buffers of varying compositions, concentrations, pHs and cofactor (PLP) concentrations. Notably, concentrations of PLP above 0.1 mM were correlated with a dramatic decrease of the immobilization yield. The highest catalytic activity, along with quantitative immobil...
Source: Journal of Biotechnology - December 12, 2018 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 20 December 2018Source: Journal of Biotechnology, Volume 288Author(s): (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - December 5, 2018 Category: Biotechnology Source Type: research

Optimization of Production of Vesicular Stomatitis Virus (VSV) in suspension serum-free culture medium at high cell density
In conclusion, our data demonstrated that 293SF-3F6 cells, for which a cGMP master cell bank is available, is a performant cell line to scale up VSV production in suspension culture using serum-free medium. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - December 1, 2018 Category: Biotechnology Source Type: research

Lactobacillus reuteri NAD(P)H oxidase: Properties and coexpression with propanediol-utilization enzymes for enhancing 3-hydroxypropionic acid production from 3-hydroxypropionaldehyde
Publication date: Available online 29 November 2018Source: Journal of BiotechnologyAuthor(s): Tarek Dishisha, Ramin Sabet-Azad, Victor Arieta, Rajni Hatti-KaulAbstractLactobacillus reuteri metabolizes glycerol through propanediol-utilization (Pdu) pathway to 1,3-propanediol (1,3-PD) via 3-hydroxypropionaldehyde (3-HPA) as intermediate. In the resting cells, the oxidized co-factor obtained in the reaction is regenerated by simultaneous oxidation of 3-HPA to 3-hydroxypropionic acid (3-HP) using propionaldehyde dehydrogenase (PduP), phosphotransacylase (PduL) and propionate kinase (PduW). We have earlier shown that the use of...
Source: Journal of Biotechnology - November 30, 2018 Category: Biotechnology Source Type: research

Enhancement of the Brenneria sp. levansucrase thermostability by site-directed mutagenesis at Glu404 located at the “-TEAP-” residue motif
Publication date: Available online 29 November 2018Source: Journal of BiotechnologyAuthor(s): Wei Xu, Jiaying Peng, Wenli Zhang, Tao Zhang, Cuie Guang, Wanmeng MuAbstractLevansucrase (EC 2.1.4.10, LS) has been used in the production of levan and levan-type fructooligosaccharides from sucrose; however, development of further application is restricted due to its poor thermostability. The LS from Brenneria sp. EniD312 was engineered using a structure-guided approach. Residue Glu404 was located in the “-TEAP-” motif and varied among LSs with different thermostabilities. Site-directed mutagenesis was performed in Gl...
Source: Journal of Biotechnology - November 30, 2018 Category: Biotechnology Source Type: research

An Enhanced Regeneration Strategy to Improve Microbial Control and Prolong RESIN Lifetime for Protein A Resin in Large-scale Monoclonal Antibody (mAb) Purification
This report introduces an enhanced regeneration strategy for MabSelect SuRe resin. The approach applies benzyl alcohol in low concentration acetic acid and NaOH (100 mM) solutions in sequential steps to regenerate MabSelect SuRe resin for reuse. Such solutions demonstrated good resin compatibility in resin functional test after extended solution incubation time, and demonstrated superior microbial control using eight test microorganisms. The cleaning effectiveness of the proposed strategy is demonstrated by carryover study, where product and impurity carryover was below detection limit. Finally, during a resin lifet...
Source: Journal of Biotechnology - November 29, 2018 Category: Biotechnology Source Type: research

Genetic modification of the carotenoid pathway in the red yeast Xanthophyllomyces dendrorhous: Engineering of a high-yield zeaxanthin strain
Publication date: Available online 26 November 2018Source: Journal of BiotechnologyAuthor(s): Jürgen Breitenbach, Hendrik Pollmann, Gerhard SandmannABSTRACTThe red yeast Xanthophyllomyces dendrorhous was genetically engineered for high-yield accumulation of the carotenoid zeaxanthin. Initially, an astaxanthin hyper-producing mutant was used to generate a β-carotene synthesizing transformant by inactivation of the astaxanthin synthase gene. Subsequently, a bacterial β-carotene hydroxylase gene was genome integrated to establish β-carotene to zeaxanthin conversion. Crucial for efficient zeaxanthin formati...
Source: Journal of Biotechnology - November 27, 2018 Category: Biotechnology Source Type: research

pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose
In this study, we found that pH differentially affects citric acid and lipid production in Y. lipolytica W29, with citric acid production enhanced at more neutral pH’s and lipid production enhanced at more acid pH’s. To determine the mechanism governing this pH-dependent switch between citric acid and lipid production, we profiled gene expression at different pH’s and found that the relative expression of multiple transporters is increased at neutral pH. These results suggest that this pH-dependent switch is mediated at the level of citric acid transport rather than changes in the expression of the enzyme...
Source: Journal of Biotechnology - November 27, 2018 Category: Biotechnology Source Type: research

Modification of Phage Display Technique for Improved Screening of High-Affinity Binding Peptides
Publication date: Available online 26 November 2018Source: Journal of BiotechnologyAuthor(s): Soi Yun, Sangah Lee, Jong Pil Park, Jaebum Choo, E.K. LeeAbstractThe phage display technique is a combinatorial technology in which random peptides are displayed on the surface of the phage; it is widely used to identify high-affinity peptides that bind to a target protein. However, this technique presents several problems due to non-specific binding of the phages and steric hindrance caused by blocking agents. To overcome these problems, we tested two modified methods and compared their screening performance with that of the conv...
Source: Journal of Biotechnology - November 27, 2018 Category: Biotechnology Source Type: research

Engineering Diverse Eubacteria Promoters for Robust Gene Expression in Streptomyces lividans
This study thus greatly expands promoter diversity for the engineering of Streptomyces. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - November 25, 2018 Category: Biotechnology Source Type: research

A fluorescent 3D cell culture assay for high throughput screening of cancer drugs down-regulating survivin
This study demonstrated the potential application of the novel 3D survivin promoter-EGFP reporter assay for high-throughput screening of chemicals down-regulating survivin as a molecular target for cancer therapy. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - November 22, 2018 Category: Biotechnology Source Type: research

Biocatalyst Engineering of Thermomyces Lanuginosus Lipase Adsorbed on Hydrophobic Supports: Modulation of Enzyme Properties for Ethanolysis of Oil in Solvent-Free Systems
Publication date: Available online 19 November 2018Source: Journal of BiotechnologyAuthor(s): Erick Abreu Silveira, Sonia Moreno-Perez, Alessandra Basso, Simona Serban, Rita Pestana-Mamede, Paulo W. Tardioli, Cristiane Sanchez-Farinas, Natalia Castejon, Gloria Fernandez-Lorente, Javier Rocha-Martin, Jose M. GuisanAbstractDifferent immobilized biocatalysts of Thermomyces lanuginosus lipase (TLL) exhibited different properties for the ethanolysis of high oleic sunflower oil in solvent-free systems. TLL immobilized by interfacial adsorption on octadecyl (C-18) supports lost its 1,3-regioselectivity and produced more than 99% ...
Source: Journal of Biotechnology - November 21, 2018 Category: Biotechnology Source Type: research

Setting-up a fast and reliable cytokinin biosensor based on a plant histidine kinase receptor expressed in Saccharomyces cerevisiae
Publication date: Available online 20 November 2018Source: Journal of BiotechnologyAuthor(s): Dimitri Daudu, Anna Kisiala, Carolina Werner Ribeiro, Céline Mélin, Laura Perrot, Marc Clastre, Vincent Courdavault, Nicolas Papon, Audrey Oudin, Martine Courtois, Thomas Dugé de Bernonville, Matthieu Gaucher, Alexandre Degrave, Arnaud Lanoue, Philippe Lanotte, Catherine Schouler, Marie-Noëlle Brisset, R.J. Neil Emery, Olivier Pichon, Sabine CarpinAbstractCytokinins (CK) have been extensively studied for their roles in plant development. Recently, they also appeared to ensure crucial functions in the path...
Source: Journal of Biotechnology - November 21, 2018 Category: Biotechnology Source Type: research

Stereoselective synthesis of a key chiral intermediate of (S)-Rivastigmine by AKR-GDH recombinant whole cells
This study provided a low-cost and efficient process for preparation of (S)-NEMCA-HEPE with high optical purity. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - November 21, 2018 Category: Biotechnology Source Type: research

Separate Production of Hydrogen and Methane from Cassava Wastewater with Added Cassava Residue under a Thermophilic Temperature in Relation to Digestibility
Publication date: Available online 20 November 2018Source: Journal of BiotechnologyAuthor(s): Sumaeth Chavadej, Tasanee Wangmor, Kiatchai Maitriwong, Pongchai Chaichirawiwat, Pramoch Rangsunvigit, Patcharee IntanooAbstractIn this research, the separate production of hydrogen (H2) and methane (CH4) from cassava wastewater with added cassava residue was investigated using a two-stage upflow anaerobic sludge blanket (UASB) system under thermophilic temperature (55 °C) in a continuous mode of operation and steady state condition. The two-stage UASB system was operated under an optimum chemical oxygen demand (COD) lo...
Source: Journal of Biotechnology - November 21, 2018 Category: Biotechnology Source Type: research

The Immobilization of Candida rugosa Lipase on the Modified Polyethersulfone with MOF Nanoparticles as an Excellent Performance Bioreactor Membrane
In this study, the modified nanocomposite membrane of polyethersulfone (PES) with NH2-MIL-101(Cr) as a metal-organic framework (MOF) is exploited for Candida rugosa lipase (CRL) immobilization. To that end, the various amounts of NH2-MIL-101(Cr) nanoparticles are blended into PES casting solution to fabricate ultrafiltration membrane via phase inversion technique. The incorporation efficiency of NH2-MIL-101(Cr) nanoparticles on the membrane morphology is investigated using various techniques, namely atomic force microscopy (AFM), X-ray diffraction (XRD), and contact angle goniometry. In terms of water pure flux and CRL imm...
Source: Journal of Biotechnology - November 18, 2018 Category: Biotechnology Source Type: research

Multicopy integrants of crt genes and co-expression of AMP deaminase improve lycopene production in Yarrowia lipolytica
In this study, the effects of copy numbers of lycopene synthesis genes, a variety of key central metabolic genes (especially AMP deaminase-encoding gene AMPD), and 5-L fermenter cultivation on lycopene production in Y. lipolytica were investigated and the engineered strains with significantly enhanced lycopene content (46-60 mg/g DCW) were achieved. It is therefore possible to make use of the obtained strains to meet the industrial demand of lycopene production on the basis of further genetic and process optimization. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - November 16, 2018 Category: Biotechnology Source Type: research

Computation-aided rational design of a halophilic choline kinase for cytidine diphosphate choline production in high-salt condition
Publication date: Available online 13 November 2018Source: Journal of BiotechnologyAuthor(s): Cheng Zheng, Zhenjian Li, Haifeng Yang, Tianyi Zhang, Huanqing Niu, Dong Liu, Junzhi Wang, Hanjie YingAbstractBiocatalysis has become the main approach to produce cytidine diphosphate choline (CDP-choline), which has been applied for treatment of acute craniocerebral injury and consciousness after brain surgery. However, salt accumulates with the production and inhibits enzyme activity, and eventually reduces yield and product accumulation rate. Our work provided a possible solution to this problem by applying a computational desi...
Source: Journal of Biotechnology - November 14, 2018 Category: Biotechnology Source Type: research

N-acetyltransferase co-expression increases α-glucosidase expression level in Pichia pastoris
This study shows that PpMpr1 has a great potential for improvement of anti-oxidative stress ability in P. pastoris and provides a promising recombinant microorganism for industrial production of proteins. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - November 12, 2018 Category: Biotechnology Source Type: research

Characterization and application of chemical-resistant polyurethane-based enzyme and whole cell compartments
This study presents the preparation and physical-chemical characterization of chemical resistant polyurethane-based compartments for biocatalytic application. The artificial compartments were prepared from an emulsion of polymer precursor and an aqueous phase that includes a biocatalytic reaction system. After curing, highly dispersed aqueous domains were obtained, which still contain the entire biocatalytic reaction system and remain fixed in the solid polymer preparation. The tensile and compression behavior of the prepared polymeric material is not significantly affected by the incorporation and facilitates excellent st...
Source: Journal of Biotechnology - November 12, 2018 Category: Biotechnology Source Type: research

Encapsulated drug system based on the gels obtained from callus cultures modified pectins
Publication date: Available online 10 November 2018Source: Journal of BiotechnologyAuthor(s): Elena A. Günter, Oxana V. Popeyko, Elena I. IstominaAbstractThe aims of this research were to obtain modified pectins of callus cultures using various culture conditions, to evaluate the relationship between the chemical characteristics of pectins, the swelling behavior and the release of prednisolone from calcium pectinate gel (CaPG) beads. An increase of the calcium concentration in the culture media correlated significantly with the rhamnogalacturonan I (RG-I) branching of the pectin. The beads from the pectin with a highe...
Source: Journal of Biotechnology - November 11, 2018 Category: Biotechnology Source Type: research

Combined Effects of Glycosylation Precursors and Lactate on the Glycoprofile of IgG Produced by CHO Cells
Publication date: Available online 10 November 2018Source: Journal of BiotechnologyAuthor(s): Liang Zhang, Andreas Castan, Joanne Stevenson, Nathalie Chatzissavidou, Francisco Vilaplana, Veronique ChotteauAbstractThe glycosylation profile of therapeutic monoclonal antibodies (mAbs) is a crucial quality parameter for industrial Immunoglobulin G (IgG) production. Several alternative carbon sources, which function as glycosylation precursors, have been reported to impact the glycosylation pattern. Since the cells give priority to glucose uptake, the presence of this substrate can lower the effects of alternative sugars on the...
Source: Journal of Biotechnology - November 11, 2018 Category: Biotechnology Source Type: research

The effect of CO2 in enhancing photosynthetic cofactor recycling for alcohol dehydrogenase mediated chiral synthesis in cyanobacteria
Publication date: Available online 6 November 2018Source: Journal of BiotechnologyAuthor(s): Annesha Sengupta, Avinash Vellore Sunder, Sujata V. Sohoni, Pramod P. WangikarAbstractThe light harvesting photosystem in cyanobacteria offers a potential pathway for the regeneration of the nicotinamide cofactor NADPH, thereby facilitating the application of cyanobacteria as excellent whole cell biocatalysts in oxidoreductase-mediated biotransformation. The use of cyanobacterial metabolism for cofactor recycling improves the atom economy of the process compared to the commonly employed enzyme-coupled cofactor recycling using enzym...
Source: Journal of Biotechnology - November 7, 2018 Category: Biotechnology Source Type: research

Optimization of chemico-physical transformation methods for various bacterial species using diverse chemical compounds and nanomaterials
Publication date: Available online 5 November 2018Source: Journal of BiotechnologyAuthor(s): Jun Ren, Dokyun Na, Seung Min YooAbstractBacterial transformation is a fundamental technology to deliver engineered plasmids into bacterial cells, which is essential in industrial protein production, chemical production, etc. Previously, we developed a simple chemico-physical transformation method that can be applied to various bacterial species. Here, to accelerate the advance of bacteria biotechnology we optimize our method by combinatorially evaluating chemical compounds (rubidium chloride, lithium acetate, cesium chloride, dime...
Source: Journal of Biotechnology - November 6, 2018 Category: Biotechnology Source Type: research

Efficient glutathione production in metabolically engineered Escherichia coli strains using constitutive promoters
In this study, the constitutive expression of GshF from Streptococcus sanguinis (GshFss) was investigated under four different constitutive promoters. Based on previous study, five genes in Escherichia coli JM109 were deleted to eliminate the degradation of precursors and GSH. The effects of gene knockout on the constitutive expression of GshFss and GSH production were evaluated by whole cell catalysis. Finally, the engineered strain JM03Pdel1 produced 24 mM glutathione with addition of 30 mM precursors in 5-L bioreactor fed-batch fermentation. The yield of GSH based on cysteine in JM03Pdel1was reached 80 % w...
Source: Journal of Biotechnology - November 3, 2018 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 10 December 2018Source: Journal of Biotechnology, Volume 287Author(s): (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - November 3, 2018 Category: Biotechnology Source Type: research

Aptazyme-mediated direct modulation of post-transcriptional sgRNA level for conditional genome editing and gene expression
In this study, a novel system was established to regulate the post-transcriptional sgRNA level by small molecule-controlled aptazyme. This system was shown to reduce the off-target effects caused by Cas9/sgRNA, while enabling precise temporal control over gene editing and regulatory activity. This new system could provide a potentially safer and more powerful tool for genome editing and therapeutic application. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - November 2, 2018 Category: Biotechnology Source Type: research

Improvement of heavy and light chain assembly by modification of heavy chain constant region 1 (CH1): Application for the construction of an anti-paclitaxel fragment antigen-binding (Fab) antibody
Publication date: Available online 31 October 2018Source: Journal of BiotechnologyAuthor(s): Gorawit Yusakul, Seiichi Sakamoto, Hiroyuki Tanaka, Satoshi MorimotoAbstractFormation of disulfide bonds between heavy and light chains is challenge for production of recombinant immunoglobulin G (IgG) and fragment antigen-binding (Fab). Insufficient formation of the bond influences productive yield and quality of recombinant IgG and Fab. To investigate how amino acid sequences of the first heavy chain constant region (CH1) effect on assembly between heavy chain (VH-CH1 or Fd) and light chain (VL-CL), the CH1 gene sequence of an an...
Source: Journal of Biotechnology - October 31, 2018 Category: Biotechnology Source Type: research

Depletion of Endogenous miRNA-378-3p Increases Peak Cell Density of CHO DP12 Cells and is Correlated with Elevated Levels of Ubiquitin Carboxyl-Terminal Hydrolase 14
This study highlights miR-378-3p as a novel engineering candidate for improving CHO cell growth. The use of subcellular fractionation also improved proteome coverage in the identification of novel miRNA targets. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - October 31, 2018 Category: Biotechnology Source Type: research

Recycling waste nutrient solution originating from the plant factory with the cultivation of newly isolated Acutodesmus species
In this study, we isolated novel microalgal species suitable for the growth in waste nutrients from a plant factory and subsequently an underwater LED photobioreactor was constructed being incorporated into the plant factory system. Finally, treated waste nutrient solution was recycled back into the plant factory and successfully used for the cultivation of plant of butterhead lettuce (Lactuca sativa L.). (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - October 30, 2018 Category: Biotechnology Source Type: research

Clone of plipastatin biosynthetic gene cluster by transformation-associated recombination technique and high efficient expression in model organism Bacillus subtilis
In this study, a scarless genetic modification method was applied to construct a heterologous expression host (Bacillus subtilis 1A751 Δpps) by knocking out the natural plipastatin genes from B. subtilis strain 1A751. The core genes for plipastatin biosynthesis from B. amyloliquefaciens HYM12 were captured and assembled with sfp and degQ using transformation-associated recombination (TAR) cloning. The resultant gene cluster was introduced into B. subtilis 1A751 Δpps to generate strain B. subtilis 1A751 Δpps amyE::ppsA–E + sfp + degQ. Its fermentation products were analyze...
Source: Journal of Biotechnology - October 26, 2018 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 20 November 2018Source: Journal of Biotechnology, Volume 286Author(s): (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - October 24, 2018 Category: Biotechnology Source Type: research

Whole-Genome Sequence of Purple Non-sulfur Bacteria, Rhodobacter sphaeroides Strain MBTLJ-8 with Improved CO2 Reduction Capacity
We reported the complete genome sequence and characteristics based on genomic information of this bacteria.Therefore, this genome sequence provides elucidation for improved CO2 fixation and enhanced physiological active compounds production, and will be used as the efficient photosynthetic bacteria for the biological CO2 reduction system. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - October 23, 2018 Category: Biotechnology Source Type: research

Effect of continuous feeding of CO2 and pH in cell concentration and product titers in hIFNγ producing HEK293 cells: Induced metabolic shift for concomitant consumption of glucose and lactate
Publication date: Available online 21 October 2018Source: Journal of BiotechnologyAuthor(s): R. Román, M. Farràs, M. Camps, I. Martínez-Monge, Pere Comas, Mariona Martínez-Espelt, M. Lecina, A. Casablancas, J.J. CairóAbstractAlthough pH control at physiological levels is generally considered as the optimal culture condition, in some cases other strategies should be taken into account for their beneficial effects on process performance. pH and CO2 levels are chemical variables that have a major impact in cell growth and product titers in cell culture since their effect on key metabolic rou...
Source: Journal of Biotechnology - October 21, 2018 Category: Biotechnology Source Type: research