Prediction of enantioselectivity of lipase catalyzed kinetic resolution using umbrella sampling
Publication date: Available online 18 July 2018Source: Journal of BiotechnologyAuthor(s): Ashwini C. Mathpati, Bhalchandra M. BhanageAbstractEnantiopure intermediates are preferred for drug synthesis in pharmaceutical industry. Lipases are widely used for chiral resolution of optically active compounds based on kinetic resolution. In the kinetic resolution, two enantiomers react with different rates in presence of a chiral catalyst or reagent, resulting in obtaining one enantiomer of great excess compared to other. The enantiomeric ratio (E) is closely related to the free energy difference (ΔΔG) of the activate...
Source: Journal of Biotechnology - July 19, 2018 Category: Biotechnology Source Type: research

Expression of a Zika virus antigen in microalgae: towards mucosal vaccine development
Publication date: Available online 18 July 2018Source: Journal of BiotechnologyAuthor(s): Verónica Araceli Márquez-Escobar, Bernardo Bañuelos-Hernández, Sergio Rosales-MendozaAbstractZika virus (ZIKV) infection has extended rapidly all over the world in the last decades affecting humans of all ages, inducing severe illness such as the autoimmune Guillain-Barré syndrome as well as fetal neurodevelopmental defects. Despite the epidemiological importance of ZIKV, today there are no commercially available drugs or vaccines to combat or prevent this infection. Microalgae are attractive hosts t...
Source: Journal of Biotechnology - July 19, 2018 Category: Biotechnology Source Type: research

Rational engineering of ornithine decarboxylase with greater selectivity for ornithine over lysine through protein network analysis
Publication date: Available online 17 July 2018Source: Journal of BiotechnologyAuthor(s): Eun Young Hong, Jin Young Kim, Roopali Upadhyay, Byung Jun Park, Jong Min Lee, Byung-Gee KimAbstractOrnithine decarboxylase (ODC) converts C5 ornithine into C4 putrescine, a monomer for polyamide synthesis. However, ODC also has minor activity towards cell metabolite C6 lysine and yields C5 cadaverine. The accumulation of cadaverine in the reaction solution causes increase in the operational cost of subsequent distillation process for putrescine purification. Here, to increase ODC substrate specificity toward ornithine over lysine, mo...
Source: Journal of Biotechnology - July 18, 2018 Category: Biotechnology Source Type: research

Scale-up of Continuous Multicolumn Chromatography for the Protein A capture step: from Bench to Clinical Manufacturing
In this study, we focused on upscaling continuous protein A chromatography from lab to pilot scale using the Cadence™ BioSMB PD and the Cadence™ BioSMB Process 80 system, respectively. Additionally, we evaluated hardware and software capability whilst running the system for 10 days non-stop using feed from a perfusion bioreactor. In terms of product quality and removal of impurities, comparable data was obtained regarding lab scale and production scale. Compared to batch mode, productivity was increased by 400 to 500%. Furthermore, the system worked accurately during the whole trial, proving its potential for t...
Source: Journal of Biotechnology - July 18, 2018 Category: Biotechnology Source Type: research

Analysis of the genome sequence of plant beneficial strain Pseudomonas sp. RU47
In this study, the genome sequence of strain RU47 was obtained and phylogenetic and comparative genome analyses were performed. Multilocus sequence analysis (MLSA) coupled with the calculation of average nucleotide identity (ANI) and in silico DNA–DNA hybridization (DDH) values suggested that strain RU47 belongs to the P. koreensis group of the P. fluorescens complex, but cannot be assigned to any known Pseudomonas species. Multiple genes and operons encoding for functions that likely contribute to its previously reported high rhizosphere competence, biocontrol of Rhizoctonia solani and plant growth promotion in soil...
Source: Journal of Biotechnology - July 18, 2018 Category: Biotechnology Source Type: research

Differential expression of microRNAs in recombinant Chinese hamster ovary cells treated with sodium butyrate using digital RNA counting
Publication date: 10 October 2018Source: Journal of Biotechnology, Volume 283Author(s): Sung-Min Lim, Sun-Hye Park, Joo-Hyoung Lee, Sun Hong Kim, Jee Yon Kim, Jeong-Ki Min, Gyun Min Lee, Yeon-Gu KimAbstractSodium butyrate (NaBu) is an efficient supplement for increasing recombinant protein production in Chinese hamster ovary (CHO) cell culture. To elucidate the effects of NaBu on miRNA expression profile in recombinant CHO (rCHO) cells, differentially expressed miRNAs in NaBu-treated rCHO cells were assessed by NanoString nCounter analysis. This result showed that eight mature mouse miRNAs (let-7b, let-7d, miR-15b, miR-25,...
Source: Journal of Biotechnology - July 18, 2018 Category: Biotechnology Source Type: research

Characterization of a new microbial Pictet-Spenglerase NscbB affording the β-carboline skeletons from Nocardiopsis synnemataformans DSM 44143
Publication date: 10 September 2018Source: Journal of Biotechnology, Volume 281Author(s): Qi Chen, Shaofei Zhang, Yunchang XieAbstractThe enzymatic Pictet-Spengler (PS) reaction, catalyzed by Pictet-Spenglerase (PSase), is the feature of β-carboline (βC) alkaloid biosynthesis. NscbB is a rare microbial PSase discovered from a cryptic β-carboline alkaloid biosynthetic gene cluster (BGC) in the Nocardiopsis synnemataformans DSM 44143 (kidney transplant patient derived) by homologous alignment with its well-characterized counterpart McbB. The biochemical analysis showed that NscbB could catalyze l-tryptophan (K...
Source: Journal of Biotechnology - July 14, 2018 Category: Biotechnology Source Type: research

The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells
Publication date: Available online 11 July 2018Source: Journal of BiotechnologyAuthor(s): Nina Bydlinski, Daniel Maresch, Valerie Schmieder, Gerald Klanert, Richard Strasser, Nicole BorthAbstractGalactosylation as part of N-glycan processing is conducted by a set of beta-1,4-galactosyltransferases (B4 GALTs), with B4 GALT1 as the dominant isoenzyme for this reaction. Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4 GALT2, B4 GALT3 and B4 GALT4, have not been studied in-depth.To increase the understanding of the protein- and site-specific a...
Source: Journal of Biotechnology - July 12, 2018 Category: Biotechnology Source Type: research

Enhancement of the antibacterial activity of an E. faecalis strain by the heterologous expression of enterocin A
Publication date: Available online 10 July 2018Source: Journal of BiotechnologyAuthor(s): Carlos Eduardo Serrano-Maldonado, Maricarmen QuirascoAbstractThe genus Enterococcus occurs as native microbiota of fermented products due to its broad environmental distribution and its resistance to salt concentrations. Enterococcus faecalis F, a non-pathogenic strain isolated from a ripened cheese, has demonstrated useful enzymatic capabilities, a probiotic behavior and antibacterial activity against some food-borne pathogens, mainly due to peptidoglycan hydrolase activity. Its use as a natural pathogen-control agent could be furthe...
Source: Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Directed modification of l-LcLDH1, an l-lactate dehydrogenase from Lactobacillus casei, to improve its specific activity and catalytic efficiency towards phenylpyruvic acid
Publication date: Available online 22 May 2018Source: Journal of BiotechnologyAuthor(s): Jian-Fang Li, Xue-Qing Li, Yan Liu, Feng-Jiao Yuan, Ting Zhang, Min-Chen Wu, Ji-Ru ZhangAbstractTo improve the specific activity and catalytic efficiency of l-LcLDH1, an NADH-dependent allosteric l-lactate dehydrogenase from L. casei, towards phenylpyruvic acid (PPA), its directed modification was conducted based on the semi-rational design. The three variant genes, Lcldh1Q88R, Lcldh1I229A and Lcldh1T235G, were constructed by whole-plasmid PCR as designed theoretically, and expressed in E. coli BL21(DE3), respectively. The purified mut...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Editorial
Publication date: Available online 6 June 2018Source: Journal of BiotechnologyAuthor(s): (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Evaluating the potential of indigenous methanogenic consortium for enhanced oil and gas recovery from high temperature depleted oil reservoir
Publication date: Available online 1 July 2018Source: Journal of BiotechnologyAuthor(s): Rohit Rathi, Meeta Lavania, Vipin Kukreti, Banwari LalAbstractIn past years, lots of research has been focussed on the indigenous bacteria and their mechanisms, which help in enhanced oil recovery. Most of the oil wells in Indian subcontinent have temperature higher than 60 °C. Also, the role of methanogenic consortia from high temperature petroleum reservoir for enhanced oil recovery (EOR) has not been explored much. Hence, in the present study methanogens isolated from thermophilic oil wells (70 °C) were evaluated for enh...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Characterization of a new microbial Pictet-Spenglerase NscbB affording the β-carboline skeletonsfrom Nocardiopsis synnemataformans DSM 44143
Publication date: Available online 4 July 2018Source: Journal of BiotechnologyAuthor(s): Qi Chen, Shaofei Zhang, Yunchang XieAbstractTheenzymatic Pictet-Spengler (PS) reaction, catalyzed by Pictet-Spenglerase (PSase), is the feature ofβ-carboline (βC) alkaloid biosynthesis. NscbB is a raremicrobial PSase discovered from a cryptic β-carboline alkaloid biosynthetic gene cluster (BGC) in the Nocardiopsis synnemataformans DSM 44143 (kidney transplant patient derived) by homologous alignment with its well-characterized counterpart McbB. Thebiochemical analysis showed that NscbB could catalyze L-tryptophan (KM =...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Enzymatic characterization of a soluble aggregate induced by N-terminal extension to a lipolytic enzyme
Publication date: Available online 4 July 2018Source: Journal of BiotechnologyAuthor(s): Ji-Min Park, Mi-Hwa Lee, Chul-Hyung Kang, Ki-Hoon Oh, Jung-Sook Lee, Jung-Hoon YoonABSTRACTA self-assembling peptide (27PEP) was isolated from an open reading frame (ORF). The ORF consisted of an unknown functional domain and a catalytic (lipolytic and phospholipolytic) domain (MPlaG) on metagenomic fosmid clone. This extension of 27 amino acids prior to the N-terminus of the catalytic domain (27PEP-MPlaG), starting at Met261, produced an aggregate of high molecular weight (> 700 kDa). Compared with MPlaG, 27PEP-MPlaG showed the s...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

A Multifunctional Tag with the Ability to Benefit the Expression, Purification, Thermostability and Activity of Recombinant Proteins
In this study, a novel multifunctional tag, S1v1 (AEAEAHAH)2, was generated from a self-assembling amphipathic peptide in the Zuotin protein sequence by replacing lysine residues with histidine residues. After fusing S1v1 at the N-terminus through a PT-linker, the expressions of polygalacturonate lyase (PGL), lipoxygenase (LOX) and green fluorescent protein (GFP) were enhanced by 3.8, 0.2 and 1.52-fold, respectively,compared to the wild-type proteins. However, the frequently used His-tag with a PT-linker had negligible effects on expression. Moreover, the three S1v1 fusions were purified with high purities and acceptable r...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Deciphering Molecular Consequences of the Proprotein Convertase 1/3 Inhibition in Macrophages for Application in Anti-tumour Immunotherapy
Publication date: Available online 7 July 2018Source: Journal of BiotechnologyAuthor(s): Franck Rodet, Alice Capuz, Tsukasa Hara, Rinaldo van Meel, Marie Duhamel, Mélanie Rose, Antonella Raffo Romero, Isabelle Fournier, Michel SalzetAbstractDuring tumour development, macrophages are recruited to the tumour site and orientated towards an anti-inflammatory phenotype. Due to their immunosuppressive function, tumour associated macrophages (TAMs) are recognized as major components in tumour progression. Changing these macrophages to a pro-inflammatory phenotype is thus extensively studied as a potential means for develop...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Synthesis of butyl oleate catalyzed by cross-linked enzyme aggregates with magnetic nanoparticles in rotating magneto-micro-reactor
This study provides a novel design that realized efficient, convenient and continuous application of CLEAs in biosynthesis, and M-CLEAs also show good promises in industry production. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Influence of hydraulic retention time and carbon loading rate on the production of algae
Publication date: Available online 7 July 2018Source: Journal of BiotechnologyAuthor(s): C.M. Fernandez-Marchante, Y. Asensio, J. Lobato, J. Villaseñor, P. Cañizares, M.A. RodrigoAbstractThis paper is focused on the assessment of the production of algae in batch bioreactors. Hydraulic retention time, carbon loading rate and light color were the inputs of the study and algae production the main output. Bioreactors were operated in semi-continuous mode and tests lasted two months, more than two times the period required to meet a steady-state response. This steady-state was verified with plateau responses in bo...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Over-expression of SINAL7 increases biomass and drought tolerance, and also delays senescence in Arabidopsis
In this study, the in vivo effects of SINAL7 on plant physiology were examined by over-expressing SINAL7 in transgenic Arabidopsis plants. Phenotypic and gene expression analyses suggest the involvement of SINAL7 in the regulation of several vegetative parameters, essentially those that affect the aerial parts of the plants. Over-expression of SINAL7 resulted in an increase in the concentrations of hexoses and sucrose, with a concommitant increase in plant biomass, particularly in the number of rosette leaves and stem thickness. Interestingly, using the CAB1 (chlorophyll ab binding protein 1) gene as a marker revealed a de...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates
In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis. The present genomic data provides new insights for lignocellulose bioconversion of white-rot fungus for the genus Pleurotus. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Extraction of astaxanthin from microalga Haematococcus pluvialis in red phase by using Generally Recognized As Safe solvents and accelerated extraction
Publication date: Available online 9 July 2018Source: Journal of BiotechnologyAuthor(s): Antonio Molino, Juri Rimauro, Patrizia Casella, Antonietta Cerbone, Vincenzo Larocca, Simeone Chianese, Despina Karatza, Sanjeet Mehariya, Angelo Ferraro, Evangelos Hristoforou, Dino MusmarraAbstractSolvent Extraction was tested to extract astaxanthin from Haematococcus pluvialis in red phase (HPR), by investigating effects of solvents, extraction pressure and temperature. Astaxanthin isomers were identified and quantified in the extract. The performances of acetone and ethanol, Generally Recognized As Safe (GRAS) solvents, were explor...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Immobilization of microalgae on the surface of new cross-linked polyethylenimine-based sorbents
We report on the use of the polyethylenimine-based (PEI) sorbents for immobilization and harvesting of microalgae (MA) cells. Specific materials assessed were porous solid polymers from highly-branched PEI synthesized by cross-linking with epichlorohydrin (ECH) or diethylene glycol diglycidyl ether (DGDE). We estimated the effect of PEI/cross-linker ratio on the MA attachment and biocompatibility of the sorbents with the MA cells. A decrease in the cross-linker percentage resulted in the enhancement of the immobilization efficiency but impaired the cell viability as was manifested by inhibition of the photosynthetic activi...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Optimization of a high-cell-density polyethylenimine transfection method for rapid protein production in CHO-EBNA1 cells
Publication date: 10 September 2018Source: Journal of Biotechnology, Volume 281Author(s): Matthew Stuible, Alina Burlacu, Sylvie Perret, Denis Brochu, Béatrice Paul-Roc, Jason Baardsnes, Martin Loignon, Eric Grazzini, Yves DurocherAbstractFor pre-clinical evaluation of biotherapeutic candidates, protein production by transient gene expression (TGE) in Chinese Hamster Ovary (CHO) cells offers important advantages, including the capability of rapidly and cost-effectively generating recombinant proteins that are highly similar to those produced in stable CHO clones. We have established a novel CHO clone (CHO-3E7) expre...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Design and testing of a highly conserved human rotavirus VP8* immunogenic peptide with potential for vaccine development
Publication date: 10 September 2018Source: Journal of Biotechnology, Volume 281Author(s): Eileena Mohanty, Budheswar Dehury, Ashok Kumar Satapathy, Bhagirathi DwibediAbstractRotavirus infection of young children particularly below five years of age resulting in severe diarhoea, is the cause of a large number of infant deaths all over the world, more so in developing countries like India. Vaccines developed against this infection in the last two decades have shown mixed results with some of them leading to complications. Oral vaccines have not been very effective in India. Significant diversity has been found in circulating...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Modification of the cell wall structure of Saccharomyces cerevisiae strains during cultivation on waste potato juice water and glycerol towards biosynthesis of functional polysaccharides
Publication date: 10 September 2018Source: Journal of Biotechnology, Volume 281Author(s): Anna Bzducha-Wróbel, Stanisław Błażejak, Marek Kieliszek, Katarzyna Pobiega, Katarzyna Falana, Monika JanowiczAbstractChanges in cell wall structure of four strains of Sacccharomyces cerevisiae species (brewer’s, baker’s and probiotic yeast) after culturing on deproteinated potato juice water (DPJW) with diverse addition of glycerol and different pH were investigated. It allowed to select conditions intensifying biosynthesis of β(1,3)/(1,6)-glucan and mannoproteins of cell walls of tested strains. Yeast cell...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Clonal variation in productivity and proteolytic clipping of an Fc-fusion protein in CHO cells: Proteomic analysis suggests a role for defective protein folding and the UPR
This study was carried out to understand more fundamentally the intracellular events that may play a role in determining why cell lines from the same cell line development project can vary with regards to the extent of Fc-fusion protein clipping. The cell lines that displayed the highest levels of clipping also produced less product than the cell lines with a lower level of clipping. In this study we applied differential quantitative label-free LC–MS/MS proteomic analysis to group clonally-derived cell lines (CDCLs) based on the level of clipping of the Fc-fusion protein. The analysis was carried out over two times p...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Speed genome editing by transient CRISPR/Cas9 targeting and large DNA fragment deletion
In this study, we have validated fluorescent protein reporter aided cell sorting which enables the isolation of maximal diversity in mutant cells. We further applied two spectrally distinct fluorescent proteins DsRed2 and ECFP as reporters for independent CRISPR/Cas9 mediated targeting, which allows for one-cell-one-well sorting of the mutant cells. Because of ultra-high efficiency of the CRISPR/Cas9 system with dual reporters and large DNA fragment deletion resulting from independent loci cleavage, monoclonal mutant cells could be easily identified by conventional PCR. In the speed genome editing method presented here, so...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Real time and in vivo pharmaceutical and environmental studies with SpiderMass instrument
Publication date: 10 September 2018Source: Journal of Biotechnology, Volume 281Author(s): B. Fatou, P. Saudemont, M. Duhamel, M. Ziskind, C. Focsa, M. Salzet, I. FournierAbstractRemote Infrared Matrix Assisted Laser Desorption Ionization (Remote IR MALDI) system (SpiderMass) with endogenous water as matrix allows to perform real-time DMPK in vivo. In this work, SpiderMass was used to analyze the impact on metabolite production or release of invalidated pro-protein PC1/3 macrophages by Short RNA (shRNA) versus scramble shRNA with Paclitaxel. Time course in vivo experiments were then performed on the inner and outer faces of...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Improving phospholipase D activity and selectivity by bio-imprinting-immobilization to produce phosphatidylglycerol
Publication date: 10 September 2018Source: Journal of Biotechnology, Volume 281Author(s): Binglin Li, Dandan Duan, Jiao Wang, Huanyu Li, Xiaoli Zhang, Binxia ZhaoAbstractPhospholipase D (PLD) was firstly hyperactivated by the bio-imprinting and, then, these hyperactivated PLD molecules were immobilized by adsorption and precipitation, followed by cross-linking. The high degree of conformational rigidification provided by intra- and intermolecular cross-linking can make PLD “remember” imprint-induced characteristics even in aqueous solutions. The obtained immobilized PLD showed the excellent catalytic performanc...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Lipase-mediated direct in situ ring-opening polymerization of ε-caprolactone formed by a chemo-enzymatic method
Publication date: 10 September 2018Source: Journal of Biotechnology, Volume 281Author(s): Yuanyuan Zhang, Peiyu Lu, Qinghua Sun, Tao Li, Lanjie Zhao, Xin Gao, Fanye Wang, Junhong LiuAbstractA novel method to synthesize poly(ε-caprolactone) (PCL) through a three-step, lipase-mediated chemo-enzymatic reaction from cyclohexanone using an immobilized lipase from Trichosporon laibacchii (T. laibacchii) CBS5791 was developed. The immobilized preparation with 1280 U· g−1 used here was obtained by a method of purification and in situ immobilization where the crude intracellular lipase (cell homogenate) was sub...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Converting lignin derived phenolic aldehydes into microbial lipid by Trichosporon cutaneum
This study took the first experimental trial on utilizing phenolic aldehydes as the sole carbon sources for microbial lipid accumulation by T. cutaneum ACCC 20271. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Carbon nanotube-lipase hybrid nanoflowers with enhanced enzyme activity and enantioselectivity
Publication date: 10 September 2018Source: Journal of Biotechnology, Volume 281Author(s): Kai Li, Jianhua Wang, Yaojia He, Miaad Adnan Abdulrazaq, Yunjun YanAbstractVarious nanoflowers are synthesized as supports for different methods of enzyme immobilization; however, the activities of these immobilized enzymes are limited because of their confinement in the nanoflowers. In order to increase the performance of nanoflowers, in this study, different protein-phosphate hybrid nanostructures were successfully synthesized and further enhanced by carbon nanotubes (CNTs) under the same conditions. Only Cu3(PO4)2 complex nanostruc...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Enhanced overexpression, purification of a channelrhodopsin and a fluorescent flux assay for its functional characterization
Publication date: 10 September 2018Source: Journal of Biotechnology, Volume 281Author(s): Hiofan Hoi, Zhigang Qi, Hang Zhou, Carlo D. MontemagnoAbstractChannelrhodopsins (ChRs) are a group of membrane proteins that allow cation flux across the cellular membrane when stimulated by light. They have been emerged as important tools in optogenetics where light is used to trigger a change in the membrane potential of live cells which induces downstream physiological cascades. There is also increased interest in their applications for generating light-responsive biomaterials. Here we have used a two-step screening protocol to dev...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Enhanced squalene biosynthesis in Yarrowia lipolytica based on metabolically engineered acetyl-CoA metabolism
In this study, we focused on the enhancement of squalene biosynthesis in Yarrowia lipolytica, with particular attention to the engineering of acetyl-CoA metabolism based on genome-scale metabolic reaction network analysis. Although the overexpression of the rate-limiting endogenous ylHMG1 (3-hydroxy-3-methylglutaryl-CoA reductase gene) could improve squalene synthesis by 3.2-fold over that by the control strain, the availability of the key intracellular precursor, acetyl-CoA, was found to play a more significant role in elevating squalene production. Analysis of metabolic networks with the newly constructed genome-scale me...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Features of the transglutaminase-activating metalloprotease from Streptomyces mobaraensis DSM 40847 produced in Escherichia coli
Publication date: 10 September 2018Source: Journal of Biotechnology, Volume 281Author(s): Norbert E. Juettner, Moritz Classen, Felix Colin, Sascha B. Hoffmann, Christian Meyners, Felicitas Pfeifer, Hans-Lothar FuchsbauerAbstractTransglutaminase from Streptomyces mobaraensis (MTG) is an important enzyme for numerous industrial applications. Recombinant production requires proteolytic activation of the zymogen. The study provides a convenient procedure for the preparation of the transglutaminase-activating metalloprotease (TAMP) in Escherichia coli. In contrast to wtTAMP, rTAMP exhibited the P domain of convertases as molecu...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Opportunities in biotechnology
Publication date: 20 September 2018Source: Journal of Biotechnology, Volume 282Author(s): Kevan M.A. Gartland, Jill S. GartlandAbstractStrategies for biotechnology must take account of opportunities for research, innovation and business growth. At a regional level, public-private collaborations provide potential for such growth and the creation of centres of excellence. By considering recent progress in areas such as genomics, healthcare diagnostics, synthetic biology, gene editing and bio-digital technologies, opportunities for smart, strategic and specialised investment are discussed. These opportunities often involve co...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Characterization of the stability of Vibrio fluvialis JS17 amine transaminase
Publication date: 20 September 2018Source: Journal of Biotechnology, Volume 282Author(s): Shan Chen, Jonatan C. Campillo-Brocal, Per Berglund, Maria Svedendahl HumbleAbstractThe amine transaminase from Vibrio fluvialis (Vf-ATA) is an attractive enzyme with applications within Biocatalysis for the preparation of chiral amines. Various catalytic properties of Vf-ATA have been investigated, but a biophysical characterization of its stability has been lacking. Today, the industrial application of Vf-ATA is limited by its low operational stability. In order to enhance the knowledge regarding the structural stability of ATAs, ge...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Improving cellulosic ethanol fermentability of Zymomonas mobilis by overexpression of sodium ion tolerance gene ZMO0119
This study screened and overexpressed the genes responsible for transporting metal ions in Z. mobilis for increasing its Na+ tolerance. The ZMO0119 gene encoding Na+/H+ antiporter was identified to be highly effective for reducing intracellular Na+ concentration of Z. mobilis by improving the Na+ transport capacity. Overexpression of ZMO0119 gene in Z. mobilis significantly accelerated the cell growth, glucose consumption, and cellulosic ethanol production from the dry acid pretreated and biodetoxified corn stover feedstock. This study provided an important gene responsible for increasing the cellulosic ethanol fermentabil...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Identification of Pseudomonas mosselii BS011 gene clusters required for suppression of Rice Blast Fungus Magnaporthe oryzae
Publication date: 20 September 2018Source: Journal of Biotechnology, Volume 282Author(s): Lijuan Wu, Wei Xiao, Guoqing Chen, Dawei Song, Maqsood Ahmed Khaskheli, Pei Li, Shiying Zhang, Guozhong FengAbstractPseudomonas is a Gram-negative, rod-shaped bacteria. Many members of this genus displayed remarkable physiological and metabolic activity against different plant pathogens. However, Pseudomonas mosselii has not yet been characterized in biocontrol against plant disease. Here we isolated a strain of P. mosselii BS011 from the rhizosphere soil of rice plants, and the isolate showed strong inhibitory activity against the ri...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Crosslinked enzyme aggregates (CLEA) of phytase with soymilk proteins
Publication date: 20 September 2018Source: Journal of Biotechnology, Volume 282Author(s): Harini Tirunagari, Shalini Basetty, Haridas B. Rode, Nitin W. FadnavisAbstractCrosslinked Enzyme Aggregates (CLEA) of phytase (E.C. 3.1.3.8) have been successfully prepared using soymilk as protein feeder. Compared to total initial activity of 4428 units used for immobilization, the recovered activity in the CLEA is enhanced to 8000 units. The CLEA retained 100% phytase activity up to 50 °C and 70% activity at 70 °C after 6 h incubation while soluble enzyme lost 50% activity at 50 °C and 80% at 70 °C. Soluble...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Genetic and phenotypic features defining industrial relevant Lactococcus lactis, L. cremoris and L. lactis biovar. diacetylactis strains
In this study, a genome-wide analysis was performed including 68 available genomes of L. lactis group strains showing the existence of two species (L. lactis and L. cremoris) and two biovars (L. lactis biovar. diacetylactis and L. cremoris biovar. lactis). The proposed classification scheme revealed coherency among phenotypic (through in silico and in vivo bacterial function profiling), phylogenomic (through maximum likelihood trees) and genomic (using overall genome sequence-based parameters) approaches. Strain biodiversity for the industrial biovar. diacetylactis was also analyzed, finding they are formed by at least thr...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Antibacterial and antifungal activity of phytosterols and methyl dehydroabietate of Norway spruce bark extracts
Publication date: 20 September 2018Source: Journal of Biotechnology, Volume 282Author(s): Zuzana Burčová, František Kreps, Mária Greifová, Michal Jablonský, Aleš Ház, Štefan Schmidt, Igor ŠurinaAbstractThe current study focuses on the analysis of in vitro biological activity of extract from bark of Norway spruce (Picea Abies), which can find potential application in food and cosmetic industry and pharmacology. Milled bark was subjected to Soxhlet extraction and supercritical fluid extraction to obtain two ethanol extracts. These extracts were further used to o...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Extraction of value-added components from food industry based and agro-forest biowastes by deep eutectic solvents
Publication date: 20 September 2018Source: Journal of Biotechnology, Volume 282Author(s): Michal Jablonský, Andrea Škulcová, Ana Malvis, Jozef ŠimaAbstractThe scientific community, experts in technology and marketing have been seeking cost-competitive and green solvents with good dissolving capacity for the valorisation of biomass and biowaste. Along with traditional solvents and techniques, deep eutectic solvents (DESs) and their bio-analogues, natural deep eutectic solvents (NADESs) are currently emerging as a new class of promising liquid media. In this review, a comprehensive summary of rece...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Over-expression of SINAL7 increases biomass and drought tolerance, and also delays senescence in Arabidopsis
In this study, the in vivo effects of SINAL7 on plant physiology were examined by over-expressing SINAL7 in transgenic Arabidopsis plants. Phenotypic and gene expression analyses suggest the involvement of SINAL7 in the regulation of several vegetative parameters, essentially those that affect the aerial parts of the plants. Over-expression of SINAL7 resulted in an increase in the concentrations of hexoses and sucrose, with a concommitant increase in plant biomass, particularly in the number of rosette leaves and stem thickness. Interestingly, using the CAB1 (chlorophyll ab binding protein 1) gene as a marker revealed a de...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates
In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis. The present genomic data provides new insights for lignocellulose bioconversion of white-rot fungus for the genus Pleurotus. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Extraction of astaxanthin from microalga Haematococcus pluvialis in red phase by using Generally Recognized As Safe solvents and accelerated extraction
Publication date: Available online 9 July 2018Source: Journal of BiotechnologyAuthor(s): Antonio Molino, Juri Rimauro, Patrizia Casella, Antonietta Cerbone, Vincenzo Larocca, Simeone Chianese, Despina Karatza, Sanjeet Mehariya, Angelo Ferraro, Evangelos Hristoforou, Dino MusmarraAbstractSolvent Extraction was tested to extract astaxanthin from Haematococcus pluvialis in red phase (HPR), by investigating effects of solvents, extraction pressure and temperature. Astaxanthin isomers were identified and quantified in the extract. The performances of acetone and ethanol, Generally Recognized As Safe (GRAS) solvents, were explor...
Source: Journal of Biotechnology - July 10, 2018 Category: Biotechnology Source Type: research

Deciphering Molecular Consequences of the Proprotein Convertase 1/3 Inhibition in Macrophages for Application in Anti-tumour Immunotherapy
Publication date: Available online 7 July 2018Source: Journal of BiotechnologyAuthor(s): Franck Rodet, Alice Capuz, Tsukasa Hara, Rinaldo van Meel, Marie Duhamel, Mélanie Rose, Antonella Raffo Romero, Isabelle Fournier, Michel SalzetAbstractDuring tumour development, macrophages are recruited to the tumour site and orientated towards an anti-inflammatory phenotype. Due to their immunosuppressive function, tumour associated macrophages (TAMs) are recognized as major components in tumour progression. Changing these macrophages to a pro-inflammatory phenotype is thus extensively studied as a potential means for develop...
Source: Journal of Biotechnology - July 8, 2018 Category: Biotechnology Source Type: research

Synthesis of butyl oleate catalyzed by cross-linked enzyme aggregates with magnetic nanoparticles in rotating magneto-micro-reactor
This study provides a novel design that realized efficient, convenient and continuous application of CLEAs in biosynthesis, and M-CLEAs also show good promises in industry production. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - July 8, 2018 Category: Biotechnology Source Type: research

Influence of hydraulic retention time and carbon loading rate on the production of algae
Publication date: Available online 7 July 2018Source: Journal of BiotechnologyAuthor(s): C.M. Fernandez-Marchante, Y. Asensio, J. Lobato, J. Villaseñor, P. Cañizares, M.A. RodrigoAbstractThis paper is focused on the assessment of the production of algae in batch bioreactors. Hydraulic retention time, carbon loading rate and light color were the inputs of the study and algae production the main output. Bioreactors were operated in semi-continuous mode and tests lasted two months, more than two times the period required to meet a steady-state response. This steady-state was verified with plateau responses in bo...
Source: Journal of Biotechnology - July 8, 2018 Category: Biotechnology Source Type: research

Directed modification of l-LcLDH1, an l-lactate dehydrogenase from Lactobacillus casei, to improve its specific activity and catalytic efficiency towards phenylpyruvic acid
Publication date: Available online 22 May 2018Source: Journal of BiotechnologyAuthor(s): Jian-Fang Li, Xue-Qing Li, Yan Liu, Feng-Jiao Yuan, Ting Zhang, Min-Chen Wu, Ji-Ru ZhangAbstractTo improve the specific activity and catalytic efficiency of l-LcLDH1, an NADH-dependent allosteric l-lactate dehydrogenase from L. casei, towards phenylpyruvic acid (PPA), its directed modification was conducted based on the semi-rational design. The three variant genes, Lcldh1Q88R, Lcldh1I229A and Lcldh1T235G, were constructed by whole-plasmid PCR as designed theoretically, and expressed in E. coli BL21(DE3), respectively. The purified mut...
Source: Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research