Clone of Plipastatin Biosynthesis Gene Cluster by Transformation-associated Recombination Technique and High Efficient Expression in Model Organism Bacillus subtilis
In this study, a scarless genetic modification method was applied to construct a heterologous expression host (Bacillus subtilis 1A751 Δpps) by knocking out the natural plipastatin genes from B. subtilis strain 1A751. The core genes for plipastatin biosynthesis from B. amyloliquefaciens HYM12 were captured and assembled with sfp and degQ using transformation-associated recombination (TAR) cloning. The resultant gene cluster was introduced into B. subtilis 1A751 Δpps to generate strain B. subtilis 1A751 Δpps amyE::ppsA–E + sfp + degQ. Its fermentation products were analyze...
Source: Journal of Biotechnology - October 20, 2018 Category: Biotechnology Source Type: research

Monitoring of Antibody-drug Conjugation Reactions with UV/Vis Spectroscopy
Publication date: Available online 12 October 2018Source: Journal of BiotechnologyAuthor(s): Sebastian Andris, Matthias Rüdt, Jonas Rogalla, Michaela Wendeler, Jürgen HubbuchAbstractThe conjugation reaction of monoclonal antibodies (mAbs) with small-molecule drugs is a central step during production of antibody-drug conjugates (ADCs). The ability to monitor this step in real time can be advantageous for process understanding and control. Here, we propose a method based on UV/Vis spectroscopy in conjunction with partial least squares (PLS) regression for non-invasive monitoring of conjugation reactions. In experim...
Source: Journal of Biotechnology - October 13, 2018 Category: Biotechnology Source Type: research

Improved conversion of Dibenzothiophene into sulfone by surface display of Dibenzothiophene monooxygenase (DszC) in recombinant Escherichia coli
Publication date: Available online 12 October 2018Source: Journal of BiotechnologyAuthor(s): Sabita Rangra, Mira Kabra, Vageesh Gupta, Preeti SrivastavaAbstractBiodesulfurization is an eco-friendly process for removing sulfur from petroleum fractions. The process could not be commercialized because of the inability of microorganisms to desulfurize a wide range of heterocyclic poly aromatic sulfur compounds like dibenzothiophene (DBT), 4, 6-Dimethyl DBT present in fuel and low desulfurization activity. In the present study, to improve the rates of conversion of dibenzothiophene to dibenzothiophene sulfone, the responsible e...
Source: Journal of Biotechnology - October 13, 2018 Category: Biotechnology Source Type: research

Freestanding ultrathin films for separation of small molecules in an aqueous environment
Publication date: Available online 11 October 2018Source: Journal of BiotechnologyAuthor(s): Agnes Rodler, Christian Schuster, Eva Berger, Rupert Tscheließnig, Alois JungbauerAbstractAlternative separation methods operating in an aqueous environment are of increasing importance for further progress of molecular separation in life sciences and other industrial sectors working towards a biobased economy. By spincoating, membranes with thicknesses under 100 nm and 20 cm2 surface area were prepared from an epoxy based resin. For the first time such ultrathin epoxy films were used for the selective separation of small mol...
Source: Journal of Biotechnology - October 11, 2018 Category: Biotechnology Source Type: research

Optimization of transglutaminase (TG) immobilization on the surface of polyethersulfone ultrafiltration membrane and its characteristics in a membrane reactor
Publication date: Available online 9 October 2018Source: Journal of BiotechnologyAuthor(s): Wang Wen-qiong, Zhang Xiao-fengAbstractThe process of microbial transglutaminase (TG) covalent immobilization on an ultrafiltration membrane surface, the optimum immobilization conditions and the characteristics of the enzymatic membrane in a reactor were investigated. The process of TG immobilization on polyethersulfone (PES) membrane surfaces was analyzed by Fourier transform infrared and X-ray photoelectron spectroscopy. The optimal condition for TG immobilization was in pH 5.0 phosphate buffer containing TG (20 U/mL). The immobi...
Source: Journal of Biotechnology - October 10, 2018 Category: Biotechnology Source Type: research

Detection and discrimination of Shigella sonnei and Shigella flexneri based on vacuolar responses in Saccharomyces cerevisiae
This study provided a system for bacteria detection based on a lysosome-like-vacuole response in the yeast Saccharomyces cerevisiae. Vacuoles are factors known to activate the immune system in the presence of foreign substances. Here, Shigella sonnei and Shigella flexneri were exposed to yeast to analyze the alteration of vacuolar enzymes. The ability to detect the bacteria was evaluated by confocal microscopy after exposing and staining vacuoles with LysoTracker. Results showed that the treatment of yeast with these bacteria increased the number of red vacuole-like organelles surrounding yeast nuclei. Thus, vacuole altera...
Source: Journal of Biotechnology - October 5, 2018 Category: Biotechnology Source Type: research

Dynamic suspension culture improves ex vivo expansion of cytokine-induced killer cells by upregulating cell activation and glucose consumption rate
Publication date: 10 December 2018Source: Journal of Biotechnology, Volume 287Author(s): Weiwei Zhang, Haibo Cai, Wen-Song TanAbstractEx vivo expansion is an effective strategy to acquire cytokine-induced killer (CIK) cells needed for clinical trials. In this work, the effects of dynamic suspension culture, which was carried out by shake flasks on a shaker, on CIK cells were investigated by the analysis of expansion characteristics and physiological functions, with the objective to optimize the culture conditions for ex vivo expansion of CIK cells. The results showed that the expansion folds of total cells in dynamic cultu...
Source: Journal of Biotechnology - October 5, 2018 Category: Biotechnology Source Type: research

Characterization of cellular states of CHO-K1 suspension cell culture through cell cycle and RNA-sequencing profiling
Publication date: Available online 19 September 2018Source: Journal of BiotechnologyAuthor(s): Ileana Tossolini, Fernando J. López-Díaz, Ricardo Kratje, Claudio C. PrietoAbstractChinese hamster ovary (CHO) derived cell lines are the preferred host system for the production of therapeutic proteins. The aim of this work was to explore the regulation of suspension-adapted CHO-K1 host cell line bioprocesses, especially under a temperature gradient from 37 °C to 31 °C. We analyzed cell cycle behavior through flow cytometry of propidium iodide stained cells and high throughput transcriptome dy...
Source: Journal of Biotechnology - September 21, 2018 Category: Biotechnology Source Type: research

Propionic acid metabolism and poly-3-hydroxybutyrate-co-3-hydroxyvalerate production by a prpC mutant of Herbaspirillum seropedicae Z69
Publication date: Available online 18 September 2018Source: Journal of BiotechnologyAuthor(s): Ana Inés Catalán, Ana Karen Malan, Fernando Ferreira, Paul R. Gill, Silvia BatistaAbstractPolyhydroxyalkanoates (PHAs) are thermoplastic polyesters produced by a wide range of bacteria as carbon and energy reserves. PHA accumulation is typically increased under unbalanced growth conditions and with carbon source in excess. Although polyhydroxybutyrate (PHB) could be used for specific applications, it is brittle and not a useful alternative for plastics like polypropylene. Far more useful polypropylene-like PHAs, are...
Source: Journal of Biotechnology - September 19, 2018 Category: Biotechnology Source Type: research

Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCR
In this study, isolated ssDNA aptamers by ST-SELEX were used for detection of SEA via apta-Real time PCR (apta-qPCR). After in silico analysis of SEA protein with SEE and finding the specific region on the surface of protein, ST-SELEX was carried out in two steps (classical SELEX and Second SELEX). Finally, after isolating high specific aptamers, the apta-qPCR was used for the detection of the SEA. In this technique, poly-clonal antibody against SEA was immobilized on protein G sepharose beads (Ab-PGs). Then, the SEA protein was captured by poly clonal antibody as the target that immobilized on sepharose beads. The isolate...
Source: Journal of Biotechnology - September 18, 2018 Category: Biotechnology Source Type: research

Exploring cellular fatty acid composition and intracellular metabolites of osmotic-tolerant mutant Lactobacillus paracasei NCBIO-M2 for highly efficient lactic acid production with high initial glucose concentration
In this study, an osmotic-tolerant mutant named Lactobacillus paracasei NCBIO01-M2 was obtained through a high-throughput screening technology, which exhibited a higher tolerance to osmotic stress due to its more flexible regulation of the unsaturated fatty acid proportion along with the intracellular compatible solute pools. The mutant successfully consumed all 248 g/L initial glucose and produced 223.7 g/L lactic acid with a productivity of 5.53 g/L/h in a single batch fermentation by the neutralizing agent strategy. Moreover, similar fermentation performances were also achieved in the open fermentat...
Source: Journal of Biotechnology - September 14, 2018 Category: Biotechnology Source Type: research

Impact of Hydrodynamics on iPSC-derived Cardiomyocyte Differentiation Processes
Publication date: Available online 10 September 2018Source: Journal of BiotechnologyAuthor(s): Jasmin J. Samaras, Bernardo Abecasis, Margarida Serra, Andrea Ducci, Martina MichelettiAbstractCardiomyocytes (CMs), derived from pluripotent stem cells (PSCs), have the potential to be used in cardiac repair. Addition of physical cues, such as electrical and mechanical stimulations, have proven to significantly effect morphology, density, cardiogenesis, maturity and functionality of differentiated CMs. This work combines rigorous fluid dynamics investigation and flow frequency analysis with iPSC differentiation experiments to id...
Source: Journal of Biotechnology - September 11, 2018 Category: Biotechnology Source Type: research

Cashew apple bagasse as new feedstock for the hydrogen production using dark fermentation process
Publication date: Available online 8 September 2018Source: Journal of BiotechnologyAuthor(s): J.S. Silva, J.S. Mendes, J.A.C. Correia, M.V.P. Rocha, L. MicoliAbstractCashew apple bagasse (CAB) has been studied as feedstock for the biohydrogen production using Clostridium roseum and the dark fermentation process. Pretreatment with alkaline hydrogen peroxide (CAB-AHP) on raw material and the acid and enzymatic hydrolysis have been taken into account to evaluate the H2 yields. Results show that the acid hydrolysate obtained from CAB produced higher H2 molar yield (HMY) (15 mmolH2/Lhydrolysate) than the acid hydrolysate from C...
Source: Journal of Biotechnology - September 9, 2018 Category: Biotechnology Source Type: research

Administration of chitosan-tripolyphosphate-DNA nanoparticles to knockdown glutamate dehydrogenase expression impairs transdeamination and gluconeogenesis in the liver
Publication date: Available online 6 September 2018Source: Journal of BiotechnologyAuthor(s): Carlos Gaspar, Jonás I. Silva-Marrero, Anna Fàbregas, Montserrat Miñarro, Josep R. Ticó, Isabel V. Baanante, Isidoro MetónAbstractGlutamate dehydrogenase (GDH) plays a major role in amino acid catabolism. To increase the current knowledge of GDH function, we analysed the effect of GDH silencing on liver intermediary metabolism from gilthead sea bream (Sparus aurata). Sequencing of GDH cDNA from S. aurata revealed high homology with its vertebrate orthologues and allowed us to design short hairpin...
Source: Journal of Biotechnology - September 7, 2018 Category: Biotechnology Source Type: research

Complete genome of Gongronella sp. w5 provides insight into its relationship with plant
Publication date: Available online 5 September 2018Source: Journal of BiotechnologyAuthor(s): Yanqun Dong, Qiuyin Sun, Yinliang Zhang, Xiaojie Wang, Pu Liu, Yazhong Xiao, Zemin FangAbstractGongronella sp. w5 (w5) is a soil fungus isolated from Anhui, China. Here we report the high-quality genome sequence of w5 and its phenotypic characteristics based on genomic information. The genome of w5 consists of 34,723,828 bp assembled into 149 scaffolds and 11,302 predicted protein-coding genes. Genome analysis suggested that w5 may possess host cell infection capacity and maybe a biotrophic fungus that relies on plant sucrose as c...
Source: Journal of Biotechnology - September 6, 2018 Category: Biotechnology Source Type: research

Localized expression of antimicrobial proteins mitigates huanglongbing symptoms in Mexican lime
In this study, the human antimicrobial peptides, lysozyme and β-defensin 2, were targeted to the vascular tissue of Mexican lime (Citrus x aurantifolia [Christm.] Swingle) by fusion to a phloem-restricted protein. Localized expression was achieved, via Agrobacterium tumefaciens-mediated transformation of the stem, which led to protein expression and mobilization within the vascular tissue of heterotrophic tissues. HLB-infected plants were monitored for 360 days. Lower bacteria titers were observed in plants expressing either β-defensin 2, lysozyme, or the combination thereof, and these plants had increased photos...
Source: Journal of Biotechnology - September 6, 2018 Category: Biotechnology Source Type: research

Identification of an alternative translation initiation site in the sequence of the commonly used Glutathione S-Transferase tag
Publication date: Available online 6 September 2018Source: Journal of BiotechnologyAuthor(s): Sarah C. Bernier, Louis-Philippe Morency, Rafael Najmanovich, Christian SalesseAbstractA large number of proteins are expressed in fusion with a tag to perform their purification. Glutathione-S-Transferase (GST) is a widely used tag to achieve passenger protein purification. Accordingly, commonly used commercial expression vectors contain the coding sequence of GST in order to express fusion proteins. However, fusion proteins are sometimes expressed in a truncated form that may result from an incomplete synthesis, proteolytic clea...
Source: Journal of Biotechnology - September 6, 2018 Category: Biotechnology Source Type: research

Engineering Trichoderma reesei Rut-C30 with the overexpression of egl1 at the ace1 locus to relieve repression on cellulase production and to adjust the ratio of cellulolytic enzymes for more efficient hydrolysis of lignocellulosic biomass
In this study, T. reesei QS305 with high endoglucanase activity was developed from T. reesei Rut-C30 by replacing the transcription repressor gene ace1 with the coding region of endoglucanase gene egl1. Compared to T. reesei Rut-C30, T. reesei QS305 showed 90.0% and 132.7% increase in the activities of total cellulases and endoglucanases under flask culture conditions. When cellulase production by T. reesei QS305 was performed in the 5-L fermentor, a cellulases activity of 10.7 FPU/mL was achieved at 108 h, 75.4% higher than that produced by T. reesei Rut-C30. Moreover, cellulases produced by T. reesei QS305 were more effi...
Source: Journal of Biotechnology - September 6, 2018 Category: Biotechnology Source Type: research

Vitamin D fortification of foods and prospective health outcomes
Publication date: Available online 31 August 2018Source: Journal of BiotechnologyAuthor(s): Anargyros N. Moulas, Maria VaiouAbstractVitamin D is essential for bone health and has significant roles in non-skeletal health and organ function. Dermal synthesis through exposure to ultraviolet B light is the major natural source of vitamin D, while only a small portion of the necessary amount can be acquired by a diet without fortified foods. In recent years, vitamin D deficiency as a result of lifestyles with inadequate sun exposure, has received increased attention due to its association with the increased risk of serious chro...
Source: Journal of Biotechnology - September 1, 2018 Category: Biotechnology Source Type: research

Biotechnology in Europe: A Challenge for Central and East European Countries
Publication date: Available online 31 August 2018Source: Journal of BiotechnologyAuthor(s): Stanislav Miertuš, Miroslav Ondrejovič, Kevan M.A. Gartland (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - September 1, 2018 Category: Biotechnology Source Type: research

Biological control of plant pathogens by Bacillus species
Publication date: Available online 30 August 2018Source: Journal of BiotechnologyAuthor(s): Djordje Fira, Ivica Dimkić, Tanja Berić, Jelena Lozo, Slaviša StankovićAbstractBacteria from the Bacillus group are microorganisms that inhabit a large number of different habitats. They are well known as producers of a wide array of antagonistic compounds of different structures, having between 5 to 8% of the total genome devoted to biosynthesis of secondary metabolites. Most important bioactive molecules from the genus Bacillus are non-ribosomally synthesized peptides and lipopeptides, polyketide compounds, bacteriocins ...
Source: Journal of Biotechnology - August 31, 2018 Category: Biotechnology Source Type: research

Genome sequence of Halomonas hydrothermalis Y2, an efficient ectoine-producer isolated from pulp mill wastewater
Publication date: Available online 30 August 2018Source: Journal of BiotechnologyAuthor(s): Qi Zhao, Yiwei Meng, Shannan Li, Peiwen Lv, Ping Xu, Chunyu YangABSTRACTHalophilic microorganisms have great potentials towards biotechnological applications. Halomonas hydrothermalis Y2 is a halotolerant and alkaliphilic strain that isolated from the Na+-rich pulp mill wastewater. The strain is dominant in the bacterial community of pulp mill wastewater and exhibits metabolic diversity in utilizing various substrates. Here we present the genome sequence of this strain, which comprises a circular chromosome 3,933,432 bp in size and ...
Source: Journal of Biotechnology - August 31, 2018 Category: Biotechnology Source Type: research

Analytical Assessment of Clonal Derivation of Eukaryotic/CHO Cell Populations
Publication date: Available online 30 August 2018Source: Journal of BiotechnologyAuthor(s): Christel Aebischer-Gumy, Pierre Moretti, Thomas Little, Martin BertschingerAbstractCell populations that are exclusively composed by descendants from a defined single parental progenitor are referred to as “monoclonal”, “clonal” or more correctly as “clonally derived cell population”. Clonal derivation of cell lines used in the manufacturing of recombinant biologics is a regulatory requirement that aims to ensure a robust process and consistent quality throughout the life cycle of a product. Clona...
Source: Journal of Biotechnology - August 31, 2018 Category: Biotechnology Source Type: research

Determination of Geometrical and Viscoelastic Properties of PLA/PHB Samples Made by Additive Manufacturing for Urethral Substitution
This study is a pilot study for 3D printing of a new material mixture potentially used as a tubular substitute for urethra replacement. This new mixture is a blend of polylactic acid (PLA) and polyhydroxybutyrate (PHB).The basic aspect that affects the 3D printing process is correct material preparation and setting of 3D printer parameters.Selection of material and printing parameters depend on printing technology. The printing technology affects material behavior during printing process. The goal of preprocessing and 3D printing process is to provide stabile conditions during manufacturing to obtain usable printed samples...
Source: Journal of Biotechnology - August 30, 2018 Category: Biotechnology Source Type: research

Formulation and application of a biosurfactant from Bacillus methylotrophicus as collector in the flotation of oily water in industrial environment
Publication date: Available online 29 August 2018Source: Journal of BiotechnologyAuthor(s): Marcos José Chaprão, Rita de Cássia Freire Soares da Silva, Raquel Diniz Rufino, Juliana Moura Luna, Valdemir Alexandre Santos, Leonie Asfora SarubboAbstractThe present study describes the formulation of a biosurfactant produced by Bacillus methylotrophicus UCP1616 and investigates its long-term stability for application as a collector in a bench-scale dissolved air flotation (DAF) prototype. For formulation, the conservative potassium sorbate was added to the biosurfactant with or without prior heat treatment a...
Source: Journal of Biotechnology - August 30, 2018 Category: Biotechnology Source Type: research

Decolourization and detoxification of monoazo dyes by laccase from the white-rot fungus Trametes versicolor
Publication date: Available online 29 August 2018Source: Journal of BiotechnologyAuthor(s): Barbora Legerská, Daniela Chmelová, Miroslav OndrejovičAbstractThe decolourization and detoxification of azo dyes (Orange 2, Acid Orange 6) by fungal laccase from Trametes versicolor were evaluated. For laccase catalysed reaction, the azonaphthol Orange 2, with 72.8 % decolourization, was degraded more rapidly than the azobenzene Acid Orange 6, with 45.3 %. The presence of hydroxyl group at o-position to azo bond in the structure of Orange 2 was more preferable than the presence of two hydroxyl groups at o- and p-posi...
Source: Journal of Biotechnology - August 30, 2018 Category: Biotechnology Source Type: research

Effects of return sludge alkaline treatment on sludge reduction in laboratory-scale anaerobic–anoxic–oxic process
Publication date: Available online 28 August 2018Source: Journal of BiotechnologyAuthor(s): Wenzhe Zhang, Benyi Xiao, YanBo Li, Yu Liu, Xuesong GuoAbstractThree alkaline treatments (pH 10, 11, and 12 for 1 h) were used to treat return sludge alone to reduce sludge production in laboratory-scale anaerobic–anoxic–oxic processes. After 99 days of operation, alkaline treatments at pH 10 and 11 led to accumulative excess sludge production and sludge yield reduction of 18.8%–31.7% and 14.7%–27.8%, respectively. However, alkaline treatment at pH 12 led to system breakdown because of sludge bulking. ...
Source: Journal of Biotechnology - August 29, 2018 Category: Biotechnology Source Type: research

Strategies for controlling CRISPR/Cas9 off-target effects and biological variations in mammalian genome editing experiments
Publication date: 20 October 2018Source: Journal of Biotechnology, Volume 284Author(s): Michelle L. Kimberland, Wangfang Hou, Adolfo Alfonso-Pecchio, Stephen Wilson, Yanhua Rao, Shu Zhang, Quinn LuAbstractThe CRISPR/Cas9 system has enabled efficient modification of genes in a variety of cellular systems for studying phenotypic effects of genetic perturbations. However, with this technology comes the inherent risk of generating off-target effects (OTEs) in addition to the desired modifications. As such, it can be difficult to conclusively determine that the observed phenotypic changes are in fact due to the intended modific...
Source: Journal of Biotechnology - August 29, 2018 Category: Biotechnology Source Type: research

Full-length human CCBE1 production and purification: leveraging bioprocess development for high quality glycosylation attributes and functionality
Publication date: Available online 27 August 2018Source: Journal of BiotechnologyAuthor(s): Marta M. Silva, Patrícia Gomes-Alves, Sara Rosa, Daniel Simão, José M. Inácio, Cristina Peixoto, Margarida Serra, José A. Belo, Paula M. AlvesAbstractCollagen and calcium-binding EGF domain-1 (CCBE1) is a secreted protein critical for lymphatic/cardiac vascular development and regeneration. However, the low efficient production of the recombinant full-length CCBE1 (rCCBE1) has been a setback for functional studies and therapeutic applications using this protein.The main goal of this work was to imp...
Source: Journal of Biotechnology - August 28, 2018 Category: Biotechnology Source Type: research

Progress in the genetic engineering of cereals to produce essential polyunsaturated fatty acids
Publication date: Available online 27 August 2018Source: Journal of BiotechnologyAuthor(s): Ján Kraic, Daniel Mihálik, Lenka Klčová, Marcela Gubišová, Tatiana Klempová, Martina Hudcovicová, Katarína Ondreičková, Michaela Mrkvová, Michaela Havrlentová, Jozef Gubiš, Milan ČertíkAbstractEssential polyunsaturated fatty acids with more than two double bonds and length of carbon chain 18-22 must be taken in the diet to prevent diseases and imbalances caused by their deficiency. Terrestrial sources of polyunsaturated fatty acids are limi...
Source: Journal of Biotechnology - August 28, 2018 Category: Biotechnology Source Type: research

Model assisted comparison of Protein A resins and multi-column chromatography for capture processes
Publication date: Available online 27 August 2018Source: Journal of BiotechnologyAuthor(s): Daniel Baur, James M. Angelo, Srinivas Chollangi, Xuankuo Xu, Thomas Müller-Späth, Na Zhang, Sanchayita Ghose, Zheng Jian Li, Massimo MorbidelliAbstractEffect of particle size (85μm vs. 50μm) on the performance of continuous capture chromatography using Protein A affinity was evaluated in combination with varied feed titers, loading flow rates and target breakthrough using a Design of Experiments (DoE) approach. In comparison to previous studies, higher cell culture titers in the order 5 - 15 g/L relevant to c...
Source: Journal of Biotechnology - August 28, 2018 Category: Biotechnology Source Type: research

Differential gene expression of a feed-spiked super-producing CHO cell line
In this study, only a single shot of feed medium was added on day 0 to a basal cell culture medium and compared to non-supplemented basal medium (feed-spiked at day 0 versus control experiments) by cultivation of a recombinant mAb expressing CHO cell line in batch mode under controlled conditions in a bioreactor. Since the feed-spike at day 0 was based on existing medium components without introducing additional supplements, a desirable process with decreased complexity was generated. Unlike cells in basal medium, feed-spiked cultures reached almost 2× higher peak cell concentrations (10 × 106 c/m...
Source: Journal of Biotechnology - August 27, 2018 Category: Biotechnology Source Type: research

Strategies for controlling off-target effects and biological variations in mammalian CRISPR/Cas9 genome editing experiments
Publication date: Available online 22 August 2018Source: Journal of BiotechnologyAuthor(s): Michelle L. Kimberland, Wangfang Hou, Adolfo Alfonso-Pecchio, Stephen Wilson, Yanhua Rao, Shu Zhang, Quinn LuAbstractThe CRISPR/Cas9 system has enabled efficient modification of genes in a variety of cellular systems for studying phenotypic effects of genetic perturbations. However, with this technology comes the inherent risk of generating off-target effects (OTEs) in addition to the desired modifications. As such, it can be difficult to conclusively determine that the observed phenotypic changes are in fact due to the intended mod...
Source: Journal of Biotechnology - August 23, 2018 Category: Biotechnology Source Type: research

Utilization of centrate for the outdoor production of marine microalgae at pilot-scale in flat-panel photobioreactors
Publication date: Available online 22 August 2018Source: Journal of BiotechnologyAuthor(s): G.I. Romero-Villegas, M. Fiamengo, F.G. Acién Fernández, E. Molina GrimaAbstractThe outdoor production of marine microalgae biomass in pilot scale flat panels photobioreactors, under not sterile conditions and using centrate as nutrients source, was studied. Experiments were performed modifying the centrate percentage, dilution rate and orientation of the photobioreactors. The strain Geitlerinema sp. was that one prevailing independently of the culture conditions. The higher productivity of 47.7 gbiomass m-2·d...
Source: Journal of Biotechnology - August 23, 2018 Category: Biotechnology Source Type: research

Complete genome sequence of Leuconostoc citreum EFEL2700, a host strain for transformation of pCB vectors
This report will facilitate the understanding and application of L. citreum EFEL2700 as a food-grade microbial cell factory. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - August 23, 2018 Category: Biotechnology Source Type: research

Dynamic metabolic response of Aspergillus niger to glucose perturbation: evidence of regulatory mechanism for reduced glucoamylase production
Publication date: Available online 19 August 2018Source: Journal of BiotechnologyAuthor(s): Chao Li, Wei Shu, Shuai Wang, Peng Liu, Yingpping Zhuang, Siliang Zhang, Jianye XiaAbstractEnvironmental gradient is an important common issue during scale-up process for protein production. To address the dynamic regulatory mechanism of Aspergillus niger being exposed to inhomogeneous glucose concentrations, glucose perturbation were experimented on the steady state of A. niger chemostat culture, and dynamic profiles of the intracellular metabolites in central carbon metabolism were tracked in a time scale of seconds. The upper gly...
Source: Journal of Biotechnology - August 20, 2018 Category: Biotechnology Source Type: research

VEGF165b Mutant with a Prolonged Half-life and Enhanced Anti-tumor Potency in a Mouse Model
Publication date: Available online 19 August 2018Source: Journal of BiotechnologyAuthor(s): Huiyong Zhang, Enchao Jia, Wenjiao Xia, Chengui Lu, Wuling ZhuAbstractVEGF165b has been shown to be an effective anti-cancer agent; however, its short half-life limits further application in the clinical field. The development of a mutant VEGF165b with a prolonged half-life is urgently needed for its future application. A mutant VEGF165b was generated by inactivation of its plasmin cleavage site. The mutant and native VEGF165b proteins without purification tags were expressed via the Pichia pastoris expression system followed by pur...
Source: Journal of Biotechnology - August 20, 2018 Category: Biotechnology Source Type: research

Encapsulating genetically modified Saccharomyces cerevisiae cells in a flow-through device towards the detection of diclofenac in wastewater
Publication date: Available online 12 August 2018Source: Journal of BiotechnologyAuthor(s): C. Schirmer, J. Posseckardt, A. Kick, K. Rebatschek, W. Fichtner, K. Ostermann, A. Schuller, G. Rödel, M. MertigAbstractRecently it has been proposed to use sensors based on genetically engineered reporter cells to perform continuous online water monitoring. Here we describe the design, assembly and performance of a novel flow-through device with immobilized genetically modified yeast cells that produce a fluorescent protein upon stimulation with diclofenac whose intensity is then detected by fluorescence microscopy. Although o...
Source: Journal of Biotechnology - August 12, 2018 Category: Biotechnology Source Type: research

Extracellular Biosynthesis of Biocompatible Cadmium sulfide Quantum Dots Using Trametes Versicolor
This study provided a suitable and efficient approach to synthesize stable biocompatible cadmium sulfide quantum dots using the fungus Trametes versicolor with great potentials in the biological and biomedical researches. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - August 12, 2018 Category: Biotechnology Source Type: research

Unmarked Genetic Manipulation in Bacillus subtilis by Natural Co-transformation
Publication date: Available online 6 August 2018Source: Journal of BiotechnologyAuthor(s): Bin Ye, Chaoyang Zhou, Leizhen Zhao, Shan Cheng, Dan Cheng, Xin YanAbstractBacillus subtilis is well known as both a model organism and as a microbial cell factory. Simple and scarless gene modification is a desirable tool for basic research and industrial applications of B. subtilis. It has been demonstrated that naturally competent strains of B. subtilis can uptake multiple different DNA molecules, a phenomenon called co-transformation. Here, we describe a co-transformation-based method for generating unmarked mutants of B. subtili...
Source: Journal of Biotechnology - August 7, 2018 Category: Biotechnology Source Type: research

Elucidation of structure-function relationship of THCA and CBDA synthase from Cannabis sativaL.
Publication date: 20 October 2018Source: Journal of Biotechnology, Volume 284Author(s): Bastian Zirpel, Oliver Kayser, Felix StehleAbstractCannabinoids are secondary natural products from the plant Cannabis sativaL. Therapeutic indications of cannabinoids currently comprise a significant area of medicinal research. We have expressed the Δ9-tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase (CBDAS) recombinantly in Komagataella phaffii and could detect eight different products with a cannabinoid scaffold after conversion of the precursor cannabigerolic acid (CBGA). Besides five products remai...
Source: Journal of Biotechnology - August 7, 2018 Category: Biotechnology Source Type: research

Cloning and characterization of a new delta-specific l-leucine dioxygenase from Anabaena variabilis
Publication date: Available online 4 August 2018Source: Journal of BiotechnologyAuthor(s): Raquel S. Correia Cordeiro, Junichi Enoki, Florian Busch, Carolin Mügge, Robert KouristAbstractOptically pure hydroxy amino acids show several bioactivities and are valuable building blocks for the pharmaceutical industry. Fe(II)/α-ketoglutarate dependent dioxygenases catalyze the hydroxylation or sulfoxidation of L-amino acids with high regio- and stereoselectivity. While several β- and γ-specific enzymes have been described, only one δ-specific hydroxylase has been reported so far. Based on its similarit...
Source: Journal of Biotechnology - August 4, 2018 Category: Biotechnology Source Type: research

Markerless genome editing in Clostridium beijerinckii using the CRISPR-Cpf1 system
This study provides valuable guidance and essential references for repurposing the CRISPR-Cpf1 system for genome engineering in other microorganisms. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - August 4, 2018 Category: Biotechnology Source Type: research

Biogas Plants as Key Units of Biorefinery Concepts: Options and their Assessment
In this study a selection of significant indicators was chosen in order to determine the environmental effectivity and economic efficiency of these biorefinery concepts by a systematic assessment of possible process schemes. Thus within this study the usability of the chosen indicators and the potential of catch crops in advanced biorefineries is assessed. (Source: Journal of Biotechnology)
Source: Journal of Biotechnology - August 3, 2018 Category: Biotechnology Source Type: research

The global role of biotechnology for non communicable disorders
Publication date: Available online 2 August 2018Source: Journal of BiotechnologyAuthor(s): Serena Zacchigna, Mauro GiaccaAbstractThe World Health Organization (WHO) has tagged non-communicable diseases (NCDs) as one of the twenty-first century’s major development challenges. NCDs account for over 15 million deaths annually and over 80 per cent of those deaths occur in developing countries and among the poorest populations. Biotechnology presents unique opportunities to improve the early diagnosis and the treatment of NCDs. This review describes the major applications of biotechnology for a better clinical management ...
Source: Journal of Biotechnology - August 3, 2018 Category: Biotechnology Source Type: research

Complete genome sequence of Agrobacterium pusense VsBac-Y9, a bacterial symbiont of the dark septate endophytic fungus Veronaeopsis simplex Y34 with potential for improving fungal colonization in roots
This study sequenced the complete genome of this V. simplex-helper bacterium using the PacBio and Illumina MiSeq platforms. Hybrid assembly using SPAdes outputted a circular chromosome, a linear chromid, and a circular plasmid for a total genome 5,321,211 bp in size with a G + C content of 59.2%. Analysis of concatenated housekeeping genes (atpD-dnaK-groEL-lepA-recA-rpoB-thrE) and calculation of average nucleotide identity, showed that VsBac-Y9 was affiliated with the species Agrobacterium pusense (syn. Rhizobium pusense). Genome analysis revealed that A. pusense VsBac-Y9 contains a series of genes responsibl...
Source: Journal of Biotechnology - August 2, 2018 Category: Biotechnology Source Type: research

Comparative phenotypic analysis of CHO clones and culture media for lactate shift
Publication date: Available online 1 August 2018Source: Journal of BiotechnologyAuthor(s): Jong Kwang Hong, Shilpa Nargund, Meiyappan Lakshmanan, Sarantos Kyriakopoulos, Do Yun Kim, Kok Siong Ang, Dawn Leong, Yuansheng Yang, Dong-Yup LeeAbstractWe explored the effects of media and clonal variation on the lactate shift which can be considered as one of the desirable features in CHO cell culture. Various culture profiles with the specific growth and antibody production rates under three different media conditions in two CHO producing clones were evaluated by resorting to multivariate statistical analysis. In most cases, glut...
Source: Journal of Biotechnology - August 2, 2018 Category: Biotechnology Source Type: research

Bench-scale production of enzymes from the hydrocarbonoclastic bacteria Alcanivorax borkumensis and biodegradation tests
This study investigates motor oil (3, 5, 7.5 and 10% (v v-1)) as a sole carbon source for the production of Alcanivorax borkumensis in shake flasks and a 5 L bench-scale fermenter in comparison to the standard media. Shake flask studies showed a significant and higher cell growth (p=0.000038), lipase (p = 0.006900) and alkane hydroxylase production (p = 0.000921) by Alcanivorax borkumensis when motor oil was used as the substrate. Based on Tukey post-hoc tests, 5% motor oil concentration was selected as the optimal substrate concentration. The 5 L fermenter experiments conducted us...
Source: Journal of Biotechnology - July 31, 2018 Category: Biotechnology Source Type: research

Plant derived triterpenes from Gypsophila elegans M.Bieb. enable non-toxic delivery of gene loaded nanoplexes
Publication date: Available online 30 July 2018Source: Journal of BiotechnologyAuthor(s): Simko Sama, Gerold Jerz, Peter Schmieder, Jan F. Joseph, Matthias F. Melzig, Alexander WengAbstractTo this date, a number of different Gypsophila species from the family of Caryophyllaceae were phytochemically characterized and tested for diverse pharmacological effects. With Gypsophila elegans M. Bieb., we investigated a scarcely explored Gypsophila species, providing a number of potential transfection enhancing triterpene saponins, and so-called sapofection agents. So far triterpene saponins have not been isolated in Gypsophila eleg...
Source: Journal of Biotechnology - July 30, 2018 Category: Biotechnology Source Type: research

Tackling destructive proteolysis of unconventionally secreted heterologous proteins in Ustilago maydis
Publication date: Available online 29 July 2018Source: Journal of BiotechnologyAuthor(s): Marius Terfrüchte, Sandra Wewetzer, Parveen Sarkari, Daniel Stollewerk, Mirita Franz-Wachtel, Boris Macek, Tino Schlepütz, Michael Feldbrügge, Jochen Büchs, Kerstin SchipperAbstractThe eukaryotic microorganism Ustilago maydis is currently being developed as an alternative protein expression platform. Protein fusion with an unconventionally secreted chitinase mediates export of heterologous proteins. The unique feature of this pathway is the circumvention of N-glycosylation. Different heterologous proteins could alr...
Source: Journal of Biotechnology - July 29, 2018 Category: Biotechnology Source Type: research