An Efficient Vector-Based CRISPR/Cas9 System in Zebrafish Cell Line
In conclusion, we established a new gene editing tool for zebrafish cell line, which could be a useful in vitro platform for high-throughput analyzing gene function in fish.PMID:38652190 | DOI:10.1007/s10126-024-10320-0 (Source: Marine Biotechnology)
Source: Marine Biotechnology - April 23, 2024 Category: Biotechnology Authors: Xiaokang Ye Jiali Lin Qiuji Chen Jiehuan Lv Chunsheng Liu Yuping Wang Shuqi Wang Xiaobo Wen Fan Lin Source Type: research
Organic Matrices of Calcium Carbonate Biominerals Improve Osteoblastic Mineralization
Mar Biotechnol (NY). 2024 Apr 23. doi: 10.1007/s10126-024-10316-w. Online ahead of print.ABSTRACTMany organisms incorporate inorganic solids into their tissues to improve functional and mechanical properties. The resulting mineralized tissues are called biominerals. Several studies have shown that nacreous biominerals induce osteoblastic extracellular mineralization. Among them, Pinctada margaritifera is well known for the ability of its organic matrix to stimulate bone cells. In this context, we aimed to study the effects of shell extracts from three other Pinctada species (Pinctada radiata, Pinctada maxima, and Pinctada ...
Source: Marine Biotechnology - April 23, 2024 Category: Biotechnology Authors: Sarah Nahle Camille Lutet-Toti Yuto Namikawa Marie-H élène Piet Alice Brion Sylvie Peyroche Michio Suzuki Fr édéric Marin Marthe Rousseau Source Type: research
Dietary Poly- β-Hydroxybutyrate Improved the Growth, Non-specific Immunity, Digestive Enzyme Activity, Intestinal Morphology, Phagocytic Activity, and Disease Resistance Against Vibrio parahaemolyticus of Pacific White Shrimp, Penaeus vannamei
This study assessed the effects of dietary supplementation of poly-β-hydroxybutyrate (PHB) on growth performance, feed efficiency, non-specific immunity, digestive enzyme capacity, phagocytic activity, hemocyte count, intestinal morphology, and disease resistance against Vibrio parahaemolyticus of Pacific white shrimp (Penaeus vannamei). Six diets were prepared by supplementing graded levels of PHB at 0.00, 0.25, 0.50, 1.00, 2.00, and 4.00% (Con, P0.25, P0.5, P1.0, P2.0, and P4.0, respectively). Triplicate groups of 90 shrimps (initial body weight 0.25 ± 0.01 g) per treatment were randomly assigned and fed an experimenta...
Source: Marine Biotechnology - April 22, 2024 Category: Biotechnology Authors: Suhyeok Kim Jaebeom Shin Nalin Medagoda Sera Choi So Yun Park Jeung-Yil Park Kyeong-Jun Lee Source Type: research
High-Temperature-Induced Differential Expression of miRNA Mediates Liver Inflammatory Response in Tsinling Lenok Trout (Brachymystax lenok tsinlingensis)
In conclusion, our study provides evidence that high-temperature stress induces liver inflammation in tsinling lenok trout through dysregulation of miRNAs. Understanding the molecular mechanisms underlying the inflammatory response in tsinling lenok trout under high-temperature stress is crucial for developing strategies to mitigate the negative impacts of environmental stressors on fish health and aquaculture production.PMID:38647909 | DOI:10.1007/s10126-024-10315-x (Source: Marine Biotechnology)
Source: Marine Biotechnology - April 22, 2024 Category: Biotechnology Authors: Xiaobin Xie Yibo Wang Fang Ma Ruilin Ma Leqiang Du Xin Chen Source Type: research
A Novel Investigation for Early Sex Determination in Alive Adult European Seabass (Dicentrarchus labrax) Using cyp19a1a, dmrt1a, and dmrt1b Genes Expression in Tail Fin tissues
This study is the first investigation for using sex-related gene expression in tail fin tissues of seabass as early sex determination without killing the fish. The European seabass (Dicentrarchus labrax) is gonochoristic and lacks distinguishable sex chromosomes, so, sex determination is referred to molecular actions for some sex-related genes on autosomal chromosomes which are well known such as cyp19a1a, dmrt1a, and dmrt1b genes which play crucial role in gonads development and sex differentiation. cyp19a1a is expressed highly in females for ovarian development and dmrt1a and dmrt1b are for testis development in males. I...
Source: Marine Biotechnology - April 22, 2024 Category: Biotechnology Authors: Samy Y El-Zaeem Amr El-Hanafy Alaa A El-Dahhar Ayaat M Elmaghraby Sara F Ghanem Amany M Hendy Source Type: research
Japanese Planocerid Flatworms: Difference in Composition of Tetrodotoxin and Its Analogs and the Effects of Ingestion by Toxin-Bearing Fishes in the Ryukyu Islands, Japan
Mar Biotechnol (NY). 2024 Apr 17. doi: 10.1007/s10126-024-10312-0. Online ahead of print.ABSTRACTTetrodotoxin (TTX), known as pufferfish toxin, is a potent neurotoxin blocking sodium channels in muscle and nerve tissues. TTX has been detected in various taxa other than pufferfish, including marine polyclad flatworms, suggesting that pufferfish toxin accumulates in fish bodies via food webs. The composition of TTX and its analogs in the flatworm Planocera multitentaculata was identical to those in wild grass puffer Takifugu alboplumbeus. Previously, Planocera sp. from Okinawa Island, Japan, were reported to possess high lev...
Source: Marine Biotechnology - April 17, 2024 Category: Biotechnology Authors: Hiroyuki Ueda Masaaki Ito Ryo Yonezawa Kentaro Hayashi Taiga Tomonou Maho Kashitani Hikaru Oyama Kyoko Shirai Rei Suo Kazutoshi Yoshitake Shigeharu Kinoshita Shuichi Asakawa Shiro Itoi Source Type: research
Japanese Planocerid Flatworms: Difference in Composition of Tetrodotoxin and Its Analogs and the Effects of Ingestion by Toxin-Bearing Fishes in the Ryukyu Islands, Japan
Mar Biotechnol (NY). 2024 Apr 17. doi: 10.1007/s10126-024-10312-0. Online ahead of print.ABSTRACTTetrodotoxin (TTX), known as pufferfish toxin, is a potent neurotoxin blocking sodium channels in muscle and nerve tissues. TTX has been detected in various taxa other than pufferfish, including marine polyclad flatworms, suggesting that pufferfish toxin accumulates in fish bodies via food webs. The composition of TTX and its analogs in the flatworm Planocera multitentaculata was identical to those in wild grass puffer Takifugu alboplumbeus. Previously, Planocera sp. from Okinawa Island, Japan, were reported to possess high lev...
Source: Marine Biotechnology - April 17, 2024 Category: Biotechnology Authors: Hiroyuki Ueda Masaaki Ito Ryo Yonezawa Kentaro Hayashi Taiga Tomonou Maho Kashitani Hikaru Oyama Kyoko Shirai Rei Suo Kazutoshi Yoshitake Shigeharu Kinoshita Shuichi Asakawa Shiro Itoi Source Type: research
Japanese Planocerid Flatworms: Difference in Composition of Tetrodotoxin and Its Analogs and the Effects of Ingestion by Toxin-Bearing Fishes in the Ryukyu Islands, Japan
Mar Biotechnol (NY). 2024 Apr 17. doi: 10.1007/s10126-024-10312-0. Online ahead of print.ABSTRACTTetrodotoxin (TTX), known as pufferfish toxin, is a potent neurotoxin blocking sodium channels in muscle and nerve tissues. TTX has been detected in various taxa other than pufferfish, including marine polyclad flatworms, suggesting that pufferfish toxin accumulates in fish bodies via food webs. The composition of TTX and its analogs in the flatworm Planocera multitentaculata was identical to those in wild grass puffer Takifugu alboplumbeus. Previously, Planocera sp. from Okinawa Island, Japan, were reported to possess high lev...
Source: Marine Biotechnology - April 17, 2024 Category: Biotechnology Authors: Hiroyuki Ueda Masaaki Ito Ryo Yonezawa Kentaro Hayashi Taiga Tomonou Maho Kashitani Hikaru Oyama Kyoko Shirai Rei Suo Kazutoshi Yoshitake Shigeharu Kinoshita Shuichi Asakawa Shiro Itoi Source Type: research
cAMP-Mediated CREM-MITF-TYR Axis Regulates Melanin Synthesis in Pacific Oysters
In this study, we performed in vitro and in vivo experiments to determine the role of cAMP in regulating melanogenesis in Pacific oysters. Besides, the function of cAMP-responsive element modulator (CREM) and the interactions between CREM and melanogenic genes were investigated. Our results showed that a high level of cAMP promotes the expression of melanogenic genes in Pacific oysters. CREM controls the expression of the MITF gene under cAMP regulation. In addition, CREM can regulate melanogenic gene expression, tyrosine metabolism, and melanin synthesis. These results indicate that cAMP plays an important role in the reg...
Source: Marine Biotechnology - April 13, 2024 Category: Biotechnology Authors: Kunyin Jiang Hong Yu Lingfeng Kong Shikai Liu Qi Li Source Type: research
cAMP-Mediated CREM-MITF-TYR Axis Regulates Melanin Synthesis in Pacific Oysters
In this study, we performed in vitro and in vivo experiments to determine the role of cAMP in regulating melanogenesis in Pacific oysters. Besides, the function of cAMP-responsive element modulator (CREM) and the interactions between CREM and melanogenic genes were investigated. Our results showed that a high level of cAMP promotes the expression of melanogenic genes in Pacific oysters. CREM controls the expression of the MITF gene under cAMP regulation. In addition, CREM can regulate melanogenic gene expression, tyrosine metabolism, and melanin synthesis. These results indicate that cAMP plays an important role in the reg...
Source: Marine Biotechnology - April 13, 2024 Category: Biotechnology Authors: Kunyin Jiang Hong Yu Lingfeng Kong Shikai Liu Qi Li Source Type: research
The lgi-miR-2d is Potentially Involved in Shell Melanin Synthesis by Targeting mitf in Manila Clam Ruditapes philippinarum
In this study, we investigated the function of lgi-miR-2d in shell melanin formation and the expression patterns of lgi-miR-2d and target gene Rpmitf in Manila clam Ruditapes philippinarum. We further explored and verified the relationship between Rpmitf and lgi-miR-2d and identified the expression level of shell color-related gene changes by RNAi and injecting the antagomir of lgi-miR-2d, respectively. Our results indicated that lgi-miR-2d antagomir affected the expression of its target gene Rpmitf. In addition, the dual-luciferase reporter assay was conducted to confirm the direct interaction between lgi-miR-2d and Rpmit...
Source: Marine Biotechnology - April 12, 2024 Category: Biotechnology Authors: Qiaoyue Xu Hongtao Nie Qianying Ma Jiadi Wang Zhongming Huo Xiwu Yan Source Type: research
The lgi-miR-2d is Potentially Involved in Shell Melanin Synthesis by Targeting mitf in Manila Clam Ruditapes philippinarum
In this study, we investigated the function of lgi-miR-2d in shell melanin formation and the expression patterns of lgi-miR-2d and target gene Rpmitf in Manila clam Ruditapes philippinarum. We further explored and verified the relationship between Rpmitf and lgi-miR-2d and identified the expression level of shell color-related gene changes by RNAi and injecting the antagomir of lgi-miR-2d, respectively. Our results indicated that lgi-miR-2d antagomir affected the expression of its target gene Rpmitf. In addition, the dual-luciferase reporter assay was conducted to confirm the direct interaction between lgi-miR-2d and Rpmit...
Source: Marine Biotechnology - April 12, 2024 Category: Biotechnology Authors: Qiaoyue Xu Hongtao Nie Qianying Ma Jiadi Wang Zhongming Huo Xiwu Yan Source Type: research
Development of a Rapid Detection Method to Prorocentrum lima by Loop-Mediated Isothermal Amplification with Hydroxy Naphthol Blue
In this study, a rapid, sensitive, and cost-effective detection method based on loop-mediated isothermal amplification (LAMP) was developed to identify P. lima. In this method, cell extracts of P. lima were diluted and used directly as templates for amplification, eliminating the need for nucleic acid purification and simplifying the detection process. Hydroxy naphthol blue (HNB) was incorporated into the reaction mix to facilitate result interpretation, enabling visual determination of the amplification outcome with the naked eye. The entire detection process, from DNA extraction to template amplification and product dete...
Source: Marine Biotechnology - April 11, 2024 Category: Biotechnology Authors: Chao Yang Yu Zhen Jialin Hou Tiezhu Mi Source Type: research
Development of a Rapid Detection Method to Prorocentrum lima by Loop-Mediated Isothermal Amplification with Hydroxy Naphthol Blue
In this study, a rapid, sensitive, and cost-effective detection method based on loop-mediated isothermal amplification (LAMP) was developed to identify P. lima. In this method, cell extracts of P. lima were diluted and used directly as templates for amplification, eliminating the need for nucleic acid purification and simplifying the detection process. Hydroxy naphthol blue (HNB) was incorporated into the reaction mix to facilitate result interpretation, enabling visual determination of the amplification outcome with the naked eye. The entire detection process, from DNA extraction to template amplification and product dete...
Source: Marine Biotechnology - April 11, 2024 Category: Biotechnology Authors: Chao Yang Yu Zhen Jialin Hou Tiezhu Mi Source Type: research
Production of Antioxidant, Angiotensin-Converting Enzyme Inhibitory and Osteogenic Gelatin Hydrolysate from Labeo rohita Swim Bladder
Mar Biotechnol (NY). 2024 Apr 1. doi: 10.1007/s10126-024-10305-z. Online ahead of print.ABSTRACTOptimization of antioxidants and angiotensin-converting enzyme (ACE) inhibitory potential gelatin hydrolysate production from Labeo rohita (rohu) swim bladder (SBGH) by alcalase using central composite design (CCD) of response surface methodology (RSM) was investigated. The maximum degree of hydrolysis (DH), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), total antioxidants (TAO), and ACE inhibitory activity were achieved at 0.1:1.0 (w/w) enzyme to substrate ratio, 61 °C hydro...
Source: Marine Biotechnology - April 1, 2024 Category: Biotechnology Authors: Balaji Wamanrao Kanwate Kalpana Patel Sandesh Suresh Karkal Deependra Rajoriya Kunal Sharan Tanaji G Kudre Source Type: research
