A Novel Kunitz-Type Serine Protease Inhibitor (HcKuSPI) is Involved in Antibacterial Defense in Innate Immunity and Participates in Shell Formation of Hyriopsis cumingii
Mar Biotechnol (NY). 2023 Dec 20. doi: 10.1007/s10126-023-10275-8. Online ahead of print.ABSTRACTSerine protease inhibitors (SPIs) are abundantly reported for its inhibition against specific proteases involved in the immune responses, but SPI data related to calcareous shells are scarce. Previously, our research group has reported the proteome analysis of non-nucleated pearl powder, and a candidate matrix protein containing two Kunitz domains in the acid soluble fraction caught our attention. In the present study, the full-length cDNA sequence of HcKuSPI was obtained from Hyriopsis cumingii. HcKuSPI was specifically expres...
Source: Marine Biotechnology - December 20, 2023 Category: Biotechnology Authors: Can Jin Kang Cheng Rui Jiang Yihang Zhang Wen Luo Source Type: research
A Novel Kunitz-Type Serine Protease Inhibitor (HcKuSPI) is Involved in Antibacterial Defense in Innate Immunity and Participates in Shell Formation of Hyriopsis cumingii
Mar Biotechnol (NY). 2023 Dec 20. doi: 10.1007/s10126-023-10275-8. Online ahead of print.ABSTRACTSerine protease inhibitors (SPIs) are abundantly reported for its inhibition against specific proteases involved in the immune responses, but SPI data related to calcareous shells are scarce. Previously, our research group has reported the proteome analysis of non-nucleated pearl powder, and a candidate matrix protein containing two Kunitz domains in the acid soluble fraction caught our attention. In the present study, the full-length cDNA sequence of HcKuSPI was obtained from Hyriopsis cumingii. HcKuSPI was specifically expres...
Source: Marine Biotechnology - December 20, 2023 Category: Biotechnology Authors: Can Jin Kang Cheng Rui Jiang Yihang Zhang Wen Luo Source Type: research
Anti-Bacterial and Immunostimulatory Properties of Ulvan-Loaded Chitosan Nanoparticles for Use in Aquaculture
Mar Biotechnol (NY). 2023 Dec 19. doi: 10.1007/s10126-023-10272-x. Online ahead of print.ABSTRACTAlternative prophylactic strategies to limit farm animal infection are needed in order to avoid the use of antibiotics. Anti-bacterial and immunostimulatory properties of bioactive compounds are of great interest in aquaculture. Marine derived polysaccharides, such as chitosan and ulvan, together with nanotechnology, have become the focus of attention in the scientific community due to their wide range of biological properties. In this work, chitosan and ulvan-loaded chitosan nanoparticles (referred as CS-TPP NPs and CS-UL-TPP ...
Source: Marine Biotechnology - December 19, 2023 Category: Biotechnology Authors: Marian Ponce Eugenia Zuasti Victoria Angu ís Catalina Fern ández-Díaz Source Type: research
Anti-Bacterial and Immunostimulatory Properties of Ulvan-Loaded Chitosan Nanoparticles for Use in Aquaculture
Mar Biotechnol (NY). 2023 Dec 19. doi: 10.1007/s10126-023-10272-x. Online ahead of print.ABSTRACTAlternative prophylactic strategies to limit farm animal infection are needed in order to avoid the use of antibiotics. Anti-bacterial and immunostimulatory properties of bioactive compounds are of great interest in aquaculture. Marine derived polysaccharides, such as chitosan and ulvan, together with nanotechnology, have become the focus of attention in the scientific community due to their wide range of biological properties. In this work, chitosan and ulvan-loaded chitosan nanoparticles (referred as CS-TPP NPs and CS-UL-TPP ...
Source: Marine Biotechnology - December 19, 2023 Category: Biotechnology Authors: Marian Ponce Eugenia Zuasti Victoria Angu ís Catalina Fern ández-Díaz Source Type: research
Physiological and Transcriptome Analysis Reveal the Regulation Mechanism Underlying the Muscle Quality Effect of Dietary Schisandra chinensis in Triploid Crucian Carp (Carassius auratus)
Mar Biotechnol (NY). 2023 Dec 11. doi: 10.1007/s10126-023-10270-z. Online ahead of print.ABSTRACTSchisandra chinensis (sc) is generally demonstrated to improve antioxidant and immune functions in mammal. The present study through physiological and transcriptome analysis revealed alterations in muscle metabolisms of triploid crucian carp (Carassius auratus) cultured at different concentrations of S. chinensis diets (sc0, sc0.125%, sc0.25%, sc0.5%, sc1%, sc2%) after 8 weeks. The serum antioxidant enzyme activities analysis showed that dietary S. chinensis could reduce oxidative stress and increase organismic antioxidant capa...
Source: Marine Biotechnology - December 11, 2023 Category: Biotechnology Authors: Anli Zuo Yonghua Zhou Yuxian Chen Sipu Liu Yuyao Lu Yingjie Li Shenping Cao Zhen Liu Source Type: research
Physiological and Transcriptome Analysis Reveal the Regulation Mechanism Underlying the Muscle Quality Effect of Dietary Schisandra chinensis in Triploid Crucian Carp (Carassius auratus)
Mar Biotechnol (NY). 2023 Dec 11. doi: 10.1007/s10126-023-10270-z. Online ahead of print.ABSTRACTSchisandra chinensis (sc) is generally demonstrated to improve antioxidant and immune functions in mammal. The present study through physiological and transcriptome analysis revealed alterations in muscle metabolisms of triploid crucian carp (Carassius auratus) cultured at different concentrations of S. chinensis diets (sc0, sc0.125%, sc0.25%, sc0.5%, sc1%, sc2%) after 8 weeks. The serum antioxidant enzyme activities analysis showed that dietary S. chinensis could reduce oxidative stress and increase organismic antioxidant capa...
Source: Marine Biotechnology - December 11, 2023 Category: Biotechnology Authors: Anli Zuo Yonghua Zhou Yuxian Chen Sipu Liu Yuyao Lu Yingjie Li Shenping Cao Zhen Liu Source Type: research
Physiological and Transcriptome Analysis Reveal the Regulation Mechanism Underlying the Muscle Quality Effect of Dietary Schisandra chinensis in Triploid Crucian Carp (Carassius auratus)
Mar Biotechnol (NY). 2023 Dec 11. doi: 10.1007/s10126-023-10270-z. Online ahead of print.ABSTRACTSchisandra chinensis (sc) is generally demonstrated to improve antioxidant and immune functions in mammal. The present study through physiological and transcriptome analysis revealed alterations in muscle metabolisms of triploid crucian carp (Carassius auratus) cultured at different concentrations of S. chinensis diets (sc0, sc0.125%, sc0.25%, sc0.5%, sc1%, sc2%) after 8 weeks. The serum antioxidant enzyme activities analysis showed that dietary S. chinensis could reduce oxidative stress and increase organismic antioxidant capa...
Source: Marine Biotechnology - December 11, 2023 Category: Biotechnology Authors: Anli Zuo Yonghua Zhou Yuxian Chen Sipu Liu Yuyao Lu Yingjie Li Shenping Cao Zhen Liu Source Type: research
Physiological and Transcriptome Analysis Reveal the Regulation Mechanism Underlying the Muscle Quality Effect of Dietary Schisandra chinensis in Triploid Crucian Carp (Carassius auratus)
Mar Biotechnol (NY). 2023 Dec 11. doi: 10.1007/s10126-023-10270-z. Online ahead of print.ABSTRACTSchisandra chinensis (sc) is generally demonstrated to improve antioxidant and immune functions in mammal. The present study through physiological and transcriptome analysis revealed alterations in muscle metabolisms of triploid crucian carp (Carassius auratus) cultured at different concentrations of S. chinensis diets (sc0, sc0.125%, sc0.25%, sc0.5%, sc1%, sc2%) after 8 weeks. The serum antioxidant enzyme activities analysis showed that dietary S. chinensis could reduce oxidative stress and increase organismic antioxidant capa...
Source: Marine Biotechnology - December 11, 2023 Category: Biotechnology Authors: Anli Zuo Yonghua Zhou Yuxian Chen Sipu Liu Yuyao Lu Yingjie Li Shenping Cao Zhen Liu Source Type: research
Physiological and Transcriptome Analysis Reveal the Regulation Mechanism Underlying the Muscle Quality Effect of Dietary Schisandra chinensis in Triploid Crucian Carp (Carassius auratus)
Mar Biotechnol (NY). 2023 Dec 11. doi: 10.1007/s10126-023-10270-z. Online ahead of print.ABSTRACTSchisandra chinensis (sc) is generally demonstrated to improve antioxidant and immune functions in mammal. The present study through physiological and transcriptome analysis revealed alterations in muscle metabolisms of triploid crucian carp (Carassius auratus) cultured at different concentrations of S. chinensis diets (sc0, sc0.125%, sc0.25%, sc0.5%, sc1%, sc2%) after 8 weeks. The serum antioxidant enzyme activities analysis showed that dietary S. chinensis could reduce oxidative stress and increase organismic antioxidant capa...
Source: Marine Biotechnology - December 11, 2023 Category: Biotechnology Authors: Anli Zuo Yonghua Zhou Yuxian Chen Sipu Liu Yuyao Lu Yingjie Li Shenping Cao Zhen Liu Source Type: research
Physiological and Transcriptome Analysis Reveal the Regulation Mechanism Underlying the Muscle Quality Effect of Dietary Schisandra chinensis in Triploid Crucian Carp (Carassius auratus)
Mar Biotechnol (NY). 2023 Dec 11. doi: 10.1007/s10126-023-10270-z. Online ahead of print.ABSTRACTSchisandra chinensis (sc) is generally demonstrated to improve antioxidant and immune functions in mammal. The present study through physiological and transcriptome analysis revealed alterations in muscle metabolisms of triploid crucian carp (Carassius auratus) cultured at different concentrations of S. chinensis diets (sc0, sc0.125%, sc0.25%, sc0.5%, sc1%, sc2%) after 8 weeks. The serum antioxidant enzyme activities analysis showed that dietary S. chinensis could reduce oxidative stress and increase organismic antioxidant capa...
Source: Marine Biotechnology - December 11, 2023 Category: Biotechnology Authors: Anli Zuo Yonghua Zhou Yuxian Chen Sipu Liu Yuyao Lu Yingjie Li Shenping Cao Zhen Liu Source Type: research
Nuclear Transformation of the Marine Pennate Diatom Nitzschia sp. Strain NIES-4635 by Multi-Pulse Electroporation
In this study, a transformation method for the marine pennate diatom Nitzschia sp. strain NIES-4635, isolated from the coastal Seto Inland Sea, was established. Plasmids containing the promoter/terminator of the fucoxanthin chlorophyll a/c binding protein gene (fcp, or Lhcf) derived from Nitzschia palea were constructed and introduced into cells by multi-pulse electroporation, resulting in 500 μg/mL nourseothricin-resistant transformants with transformation frequencies of up to 365 colonies per 108 cells. In addition, when transformation was performed using a new plasmid containing a promoter derived from a diatom-infecti...
Source: Marine Biotechnology - December 10, 2023 Category: Biotechnology Authors: Koki Okada Yu Morimoto Yukine Shiraishi Takashi Tamura Shigeki Mayama Takashi Kadono Masao Adachi Kentaro Ifuku Michiko Nemoto Source Type: research
Nuclear Transformation of the Marine Pennate Diatom Nitzschia sp. Strain NIES-4635 by Multi-Pulse Electroporation
In this study, a transformation method for the marine pennate diatom Nitzschia sp. strain NIES-4635, isolated from the coastal Seto Inland Sea, was established. Plasmids containing the promoter/terminator of the fucoxanthin chlorophyll a/c binding protein gene (fcp, or Lhcf) derived from Nitzschia palea were constructed and introduced into cells by multi-pulse electroporation, resulting in 500 μg/mL nourseothricin-resistant transformants with transformation frequencies of up to 365 colonies per 108 cells. In addition, when transformation was performed using a new plasmid containing a promoter derived from a diatom-infecti...
Source: Marine Biotechnology - December 10, 2023 Category: Biotechnology Authors: Koki Okada Yu Morimoto Yukine Shiraishi Takashi Tamura Shigeki Mayama Takashi Kadono Masao Adachi Kentaro Ifuku Michiko Nemoto Source Type: research
siRNA Silencing of FpVtg Induces Ovarian Cell Apoptosis in Redtail Prawn, Fenneropenaeus penicillatus
This study found that FpVtg was specifically expressed in F. penicillatus hepatopancreas and ovary. FpVtg was localized predominantly in the oocyte cytoplasm and distributed uniformly in the hepatopancreas tissue. Silencing FpVtg led to apoptosis in both hepatopancreas and ovary tissues. Furthermore, FpVtg depletion upregulated the expression of ovarian peritrophin 1, ovarian peritrophin 2, serine proteinase inhibitor 6, and juvenile hormone esterase-like carboxylesterase 1, while downregulated that of vitellogenin, delta-9 desaturase, and insulin-like receptor. KEGG pathway analysis implicated such as PI3K-AKT signaling, ...
Source: Marine Biotechnology - November 27, 2023 Category: Biotechnology Authors: Kianann Tan Yaxin Dong Karsoon Tan Leong-Seng Lim Khor Waiho Jing Chen Peng Xu Kit Yue Kwan Source Type: research
siRNA Silencing of FpVtg Induces Ovarian Cell Apoptosis in Redtail Prawn, Fenneropenaeus penicillatus
This study found that FpVtg was specifically expressed in F. penicillatus hepatopancreas and ovary. FpVtg was localized predominantly in the oocyte cytoplasm and distributed uniformly in the hepatopancreas tissue. Silencing FpVtg led to apoptosis in both hepatopancreas and ovary tissues. Furthermore, FpVtg depletion upregulated the expression of ovarian peritrophin 1, ovarian peritrophin 2, serine proteinase inhibitor 6, and juvenile hormone esterase-like carboxylesterase 1, while downregulated that of vitellogenin, delta-9 desaturase, and insulin-like receptor. KEGG pathway analysis implicated such as PI3K-AKT signaling, ...
Source: Marine Biotechnology - November 27, 2023 Category: Biotechnology Authors: Kianann Tan Yaxin Dong Karsoon Tan Leong-Seng Lim Khor Waiho Jing Chen Peng Xu Kit Yue Kwan Source Type: research
siRNA Silencing of FpVtg Induces Ovarian Cell Apoptosis in Redtail Prawn, Fenneropenaeus penicillatus
This study found that FpVtg was specifically expressed in F. penicillatus hepatopancreas and ovary. FpVtg was localized predominantly in the oocyte cytoplasm and distributed uniformly in the hepatopancreas tissue. Silencing FpVtg led to apoptosis in both hepatopancreas and ovary tissues. Furthermore, FpVtg depletion upregulated the expression of ovarian peritrophin 1, ovarian peritrophin 2, serine proteinase inhibitor 6, and juvenile hormone esterase-like carboxylesterase 1, while downregulated that of vitellogenin, delta-9 desaturase, and insulin-like receptor. KEGG pathway analysis implicated such as PI3K-AKT signaling, ...
Source: Marine Biotechnology - November 27, 2023 Category: Biotechnology Authors: Kianann Tan Yaxin Dong Karsoon Tan Leong-Seng Lim Khor Waiho Jing Chen Peng Xu Kit Yue Kwan Source Type: research
