Vanadium Accumulation and Reduction by Vanadium-Accumulating Bacteria Isolated from the Intestinal Contents of Ciona robusta
In this study, we discovered two bacterial strains that accumulate large amounts of vanadium, CD2-88 and CD2-102, which belong to the genera Pseudoalteromonas and Vibrio, respectively. The growth medium composition impacted vanadium uptake. Furthermore, pH was also an important factor in the accumulation and localization of vanadium. Most of the vanadium(V) accumulated by these bacteria was converted to less toxic vanadium(IV). Our results provide insights into vanadium accumulation and reduction by bacteria isolated from the ascidian C. robusta to further study the relations between ascidians and microbes and their possib...
Source: Marine Biotechnology - March 7, 2024 Category: Biotechnology Authors: Dewi Yuliani Fumihiro Morishita Takuya Imamura Tatsuya Ueki Source Type: research
Vanadium Accumulation and Reduction by Vanadium-Accumulating Bacteria Isolated from the Intestinal Contents of Ciona robusta
In this study, we discovered two bacterial strains that accumulate large amounts of vanadium, CD2-88 and CD2-102, which belong to the genera Pseudoalteromonas and Vibrio, respectively. The growth medium composition impacted vanadium uptake. Furthermore, pH was also an important factor in the accumulation and localization of vanadium. Most of the vanadium(V) accumulated by these bacteria was converted to less toxic vanadium(IV). Our results provide insights into vanadium accumulation and reduction by bacteria isolated from the ascidian C. robusta to further study the relations between ascidians and microbes and their possib...
Source: Marine Biotechnology - March 7, 2024 Category: Biotechnology Authors: Dewi Yuliani Fumihiro Morishita Takuya Imamura Tatsuya Ueki Source Type: research
DNA Methylation Analysis Reveals Potential Mechanism in Takifugu rubripes Against Cryptocaryon irritans Infection
Mar Biotechnol (NY). 2024 Mar 6. doi: 10.1007/s10126-024-10296-x. Online ahead of print.ABSTRACTTakifugu rubripes (T. rubripes) is a valuable commercial fish, and Cryptocaryon irritans (C. irritans) has a significant impact on its aquaculture productivity. DNA methylation is one of the earliest discovered ways of gene epigenetic modification and also an important form of modification, as well as an essential type of alteration that regulates gene expression, including immune response. To further explore the anti-infection mechanism of T. rubripes in inhibiting this disease, we determined genome-wide DNA methylation profile...
Source: Marine Biotechnology - March 6, 2024 Category: Biotechnology Authors: Yu-Qing Xia Yi Yang Yan-Yun Liu Jian-Xin Cheng Ying Liu Cheng-Hua Li Peng-Fei Liu Source Type: research
DNA Methylation Analysis Reveals Potential Mechanism in Takifugu rubripes Against Cryptocaryon irritans Infection
Mar Biotechnol (NY). 2024 Mar 6. doi: 10.1007/s10126-024-10296-x. Online ahead of print.ABSTRACTTakifugu rubripes (T. rubripes) is a valuable commercial fish, and Cryptocaryon irritans (C. irritans) has a significant impact on its aquaculture productivity. DNA methylation is one of the earliest discovered ways of gene epigenetic modification and also an important form of modification, as well as an essential type of alteration that regulates gene expression, including immune response. To further explore the anti-infection mechanism of T. rubripes in inhibiting this disease, we determined genome-wide DNA methylation profile...
Source: Marine Biotechnology - March 6, 2024 Category: Biotechnology Authors: Yu-Qing Xia Yi Yang Yan-Yun Liu Jian-Xin Cheng Ying Liu Cheng-Hua Li Peng-Fei Liu Source Type: research
Association of a Skin Dressing Made With the Organic Part of Marine Sponges and Photobiomodulation on the Wound Healing in an Animal Model
Mar Biotechnol (NY). 2024 Mar 5. doi: 10.1007/s10126-024-10295-y. Online ahead of print.ABSTRACTThe present study aims to characterize and to evaluate the biological effects of a skin dressing manufactured with the organic part of the Chondrilla caribensis marine sponge (called spongin-like collagen (SC)) associated or not to photobiomodulation (PBM) on the skin wound healing of rats. Skin dressings were manufactured with SC and it was characterized using scanning electron microscopy (SEM) and a tensile assay. In order to evaluate its biological effects, an experimental model of cutaneous wounds was surgically performed. E...
Source: Marine Biotechnology - March 5, 2024 Category: Biotechnology Authors: Abdias Fernando Simon Sales Karolyne Dos Santos Jorge Sousa Amanda de Souza Mirian Bonifacio Tiago Akira Tashiro Ara újo Matheus de Almeida Cruz M árcia Busanello Costa Daniel Araki Ribeiro L ívia Assis Cintia Cristina Santi Martignago Ana Cl áudia Re Source Type: research
Association of a Skin Dressing Made With the Organic Part of Marine Sponges and Photobiomodulation on the Wound Healing in an Animal Model
Mar Biotechnol (NY). 2024 Mar 5. doi: 10.1007/s10126-024-10295-y. Online ahead of print.ABSTRACTThe present study aims to characterize and to evaluate the biological effects of a skin dressing manufactured with the organic part of the Chondrilla caribensis marine sponge (called spongin-like collagen (SC)) associated or not to photobiomodulation (PBM) on the skin wound healing of rats. Skin dressings were manufactured with SC and it was characterized using scanning electron microscopy (SEM) and a tensile assay. In order to evaluate its biological effects, an experimental model of cutaneous wounds was surgically performed. E...
Source: Marine Biotechnology - March 5, 2024 Category: Biotechnology Authors: Abdias Fernando Simon Sales Karolyne Dos Santos Jorge Sousa Amanda de Souza Mirian Bonifacio Tiago Akira Tashiro Ara újo Matheus de Almeida Cruz M árcia Busanello Costa Daniel Araki Ribeiro L ívia Assis Cintia Cristina Santi Martignago Ana Cl áudia Re Source Type: research
Characterization and Biological Activities of the Ulvan Polysaccharide-Rich Fraction Obtained from Ulva rigida and Ulva pseudorotundata and Their Potential for Pharmaceutical Application
Mar Biotechnol (NY). 2024 Mar 2. doi: 10.1007/s10126-024-10299-8. Online ahead of print.ABSTRACTSeaweed from the genus Ulva (Ulvales, Chlorophyta) has a worldwide distribution and represents a potential biomass source for biotechnological applications. In the present study, we investigated the ulvan polysaccharide-rich fraction (UPRF) isolated from two Ulva species (U. rigida and U. pseudorotundata), naturally occurring on the Spanish Mediterranean coast. Chemical characterization of UPRFs was performed in order to explore the polysaccharides' composition. Biological assessments of UPRFs were compared by antioxidant activi...
Source: Marine Biotechnology - March 2, 2024 Category: Biotechnology Authors: Tha ís Fávero Massocato V íctor Robles-Carnero Bruna Rodrigues Moreira Pablo Castro-Varela Jos é Bonomi-Barufi Roberto Abdala-D íaz Leonardo Rubi R örig F élix Diego López Figueroa Source Type: research
Semi-purified Antimicrobial Proteins from Oyster Hemolymph Inhibit Pneumococcal Infection
This study determined the in vitro antibacterial activity of hemolymph and unique protein fractions from an Australian oyster (Saccostrea glomerata) against multi-drug-resistant S. pneumoniae. We developed a successful method for hemolymph extraction and separation into 16 fractions by preparative HPLC. The strongest activity was observed in fraction 7: at 42 µg/mL protein, this fraction was bactericidal to S. pneumoniae and inhibited biofilm formation. Proteomic analysis showed that fraction 7 contained relatively high abundance of carbonic anhydrase, cofilin, cystatin B-like, and gelsolin-like proteins, while surroundin...
Source: Marine Biotechnology - March 2, 2024 Category: Biotechnology Authors: Kate Summer Lei Liu Qi Guo Bronwyn Barkla Kirsten Benkendorff Source Type: research
Characterization and Biological Activities of the Ulvan Polysaccharide-Rich Fraction Obtained from Ulva rigida and Ulva pseudorotundata and Their Potential for Pharmaceutical Application
Mar Biotechnol (NY). 2024 Mar 2. doi: 10.1007/s10126-024-10299-8. Online ahead of print.ABSTRACTSeaweed from the genus Ulva (Ulvales, Chlorophyta) has a worldwide distribution and represents a potential biomass source for biotechnological applications. In the present study, we investigated the ulvan polysaccharide-rich fraction (UPRF) isolated from two Ulva species (U. rigida and U. pseudorotundata), naturally occurring on the Spanish Mediterranean coast. Chemical characterization of UPRFs was performed in order to explore the polysaccharides' composition. Biological assessments of UPRFs were compared by antioxidant activi...
Source: Marine Biotechnology - March 2, 2024 Category: Biotechnology Authors: Tha ís Fávero Massocato V íctor Robles-Carnero Bruna Rodrigues Moreira Pablo Castro-Varela Jos é Bonomi-Barufi Roberto Abdala-D íaz Leonardo Rubi R örig F élix Diego López Figueroa Source Type: research
Semi-purified Antimicrobial Proteins from Oyster Hemolymph Inhibit Pneumococcal Infection
This study determined the in vitro antibacterial activity of hemolymph and unique protein fractions from an Australian oyster (Saccostrea glomerata) against multi-drug-resistant S. pneumoniae. We developed a successful method for hemolymph extraction and separation into 16 fractions by preparative HPLC. The strongest activity was observed in fraction 7: at 42 µg/mL protein, this fraction was bactericidal to S. pneumoniae and inhibited biofilm formation. Proteomic analysis showed that fraction 7 contained relatively high abundance of carbonic anhydrase, cofilin, cystatin B-like, and gelsolin-like proteins, while surroundin...
Source: Marine Biotechnology - March 2, 2024 Category: Biotechnology Authors: Kate Summer Lei Liu Qi Guo Bronwyn Barkla Kirsten Benkendorff Source Type: research
Transcriptome Analysis of Host Anti-Vibrio harveyi Infection Revealed the Pathogenicity of V. harveyi to American Eel (Anguilla rostrata)
This study aimed to determine the LD50 of V. harveyi in American eel and assess the bacterial load in the liver, spleen, and kidney post-infection with the LD50 dose. The results showed that the LD50 of V. harveyi via intraperitoneal injection in American eels over a 14d period was determined to be 1.24 × 103 cfu/g body weight (6.2 × 104 cfu/fish). The peak bacterial load occurred at 36 h post-infection (hpi) in all three organs examined. Histopathology analysis revealed hepatic vein congestion and thrombi, tubular vacuolar degeneration, and splenic bleeding. Moreover, quantitative reverse transcription polymerase chain ...
Source: Marine Biotechnology - February 17, 2024 Category: Biotechnology Authors: Minxia Chen Qijuan Wan Ming Xu Zihao Chen Songlin Guo Source Type: research
Transcriptome Analysis of Host Anti-Vibrio harveyi Infection Revealed the Pathogenicity of V. harveyi to American Eel (Anguilla rostrata)
This study aimed to determine the LD50 of V. harveyi in American eel and assess the bacterial load in the liver, spleen, and kidney post-infection with the LD50 dose. The results showed that the LD50 of V. harveyi via intraperitoneal injection in American eels over a 14d period was determined to be 1.24 × 103 cfu/g body weight (6.2 × 104 cfu/fish). The peak bacterial load occurred at 36 h post-infection (hpi) in all three organs examined. Histopathology analysis revealed hepatic vein congestion and thrombi, tubular vacuolar degeneration, and splenic bleeding. Moreover, quantitative reverse transcription polymerase chain ...
Source: Marine Biotechnology - February 17, 2024 Category: Biotechnology Authors: Minxia Chen Qijuan Wan Ming Xu Zihao Chen Songlin Guo Source Type: research
Transcriptome Analysis of Host Anti-Vibrio harveyi Infection Revealed the Pathogenicity of V. harveyi to American Eel (Anguilla rostrata)
This study aimed to determine the LD50 of V. harveyi in American eel and assess the bacterial load in the liver, spleen, and kidney post-infection with the LD50 dose. The results showed that the LD50 of V. harveyi via intraperitoneal injection in American eels over a 14d period was determined to be 1.24 × 103 cfu/g body weight (6.2 × 104 cfu/fish). The peak bacterial load occurred at 36 h post-infection (hpi) in all three organs examined. Histopathology analysis revealed hepatic vein congestion and thrombi, tubular vacuolar degeneration, and splenic bleeding. Moreover, quantitative reverse transcription polymerase chain ...
Source: Marine Biotechnology - February 17, 2024 Category: Biotechnology Authors: Minxia Chen Qijuan Wan Ming Xu Zihao Chen Songlin Guo Source Type: research
Transcriptome Analysis of Host Anti-Vibrio harveyi Infection Revealed the Pathogenicity of V. harveyi to American Eel (Anguilla rostrata)
This study aimed to determine the LD50 of V. harveyi in American eel and assess the bacterial load in the liver, spleen, and kidney post-infection with the LD50 dose. The results showed that the LD50 of V. harveyi via intraperitoneal injection in American eels over a 14d period was determined to be 1.24 × 103 cfu/g body weight (6.2 × 104 cfu/fish). The peak bacterial load occurred at 36 h post-infection (hpi) in all three organs examined. Histopathology analysis revealed hepatic vein congestion and thrombi, tubular vacuolar degeneration, and splenic bleeding. Moreover, quantitative reverse transcription polymerase chain ...
Source: Marine Biotechnology - February 17, 2024 Category: Biotechnology Authors: Minxia Chen Qijuan Wan Ming Xu Zihao Chen Songlin Guo Source Type: research
Transcriptome Analysis of Host Anti-Vibrio harveyi Infection Revealed the Pathogenicity of V. harveyi to American Eel (Anguilla rostrata)
This study aimed to determine the LD50 of V. harveyi in American eel and assess the bacterial load in the liver, spleen, and kidney post-infection with the LD50 dose. The results showed that the LD50 of V. harveyi via intraperitoneal injection in American eels over a 14d period was determined to be 1.24 × 103 cfu/g body weight (6.2 × 104 cfu/fish). The peak bacterial load occurred at 36 h post-infection (hpi) in all three organs examined. Histopathology analysis revealed hepatic vein congestion and thrombi, tubular vacuolar degeneration, and splenic bleeding. Moreover, quantitative reverse transcription polymerase chain ...
Source: Marine Biotechnology - February 17, 2024 Category: Biotechnology Authors: Minxia Chen Qijuan Wan Ming Xu Zihao Chen Songlin Guo Source Type: research
