Screen and Optimization of an Aptamer for Alexandrium tamarense-A Common Toxin-Producing Harmful Alga
In this study, an ssDNA library was first obtained by whole cell systematic evolution of ligands by exponential enrichment after 18 consecutive rounds of iterative screening. After sequencing in combination with subsequent multiple alignment of sequences and secondary structure simulation, the library could be classified into 2 families, namely, Family1 and Family2, according to sequence similarity. Flow cytometry was used to test the affinity and cross-reactivity of Ata19, Ata6, Ata25 and Ata29 belonging to Family2. Ata19 was selected to be modified by truncation, through which a new resultant aptamer named as Ata19-1-1 w...
Source: Marine Biotechnology - September 24, 2023 Category: Biotechnology Authors: Yu Duan Fuguo Liu Chunyun Zhang Yuanyuan Wang Guofu Chen Source Type: research
Screen and Optimization of an Aptamer for Alexandrium tamarense-A Common Toxin-Producing Harmful Alga
In this study, an ssDNA library was first obtained by whole cell systematic evolution of ligands by exponential enrichment after 18 consecutive rounds of iterative screening. After sequencing in combination with subsequent multiple alignment of sequences and secondary structure simulation, the library could be classified into 2 families, namely, Family1 and Family2, according to sequence similarity. Flow cytometry was used to test the affinity and cross-reactivity of Ata19, Ata6, Ata25 and Ata29 belonging to Family2. Ata19 was selected to be modified by truncation, through which a new resultant aptamer named as Ata19-1-1 w...
Source: Marine Biotechnology - September 24, 2023 Category: Biotechnology Authors: Yu Duan Fuguo Liu Chunyun Zhang Yuanyuan Wang Guofu Chen Source Type: research
Transcriptomic Variation of Amphiprion Percula (Lacep ède, 1802) in Response to Infection with Cryptocaryon Irritans Brown, 1951
Mar Biotechnol (NY). 2023 Sep 11. doi: 10.1007/s10126-023-10246-z. Online ahead of print.ABSTRACTCryptocaryon irritans (Brown 1951) frequently infect the Pomacentridae fishes causing severe economic losses. However, the anti-C. irritans' molecular mechanism in these fishes remains largely unknown. To address this issue, we conducted RNA-Seq for C. irrtians-infected gills of the clownfish Amphiprion percula (Lacepède 1802) at the early (day 1) and late (day 3) stages of infection. A total of 1655 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pat...
Source: Marine Biotechnology - September 11, 2023 Category: Biotechnology Authors: Jose Priya T A Charutha Karunakaran Aishwarya Nath Sudha Kappalli Source Type: research
Transcriptomic Variation of Amphiprion Percula (Lacep ède, 1802) in Response to Infection with Cryptocaryon Irritans Brown, 1951
Mar Biotechnol (NY). 2023 Sep 11. doi: 10.1007/s10126-023-10246-z. Online ahead of print.ABSTRACTCryptocaryon irritans (Brown 1951) frequently infect the Pomacentridae fishes causing severe economic losses. However, the anti-C. irritans' molecular mechanism in these fishes remains largely unknown. To address this issue, we conducted RNA-Seq for C. irrtians-infected gills of the clownfish Amphiprion percula (Lacepède 1802) at the early (day 1) and late (day 3) stages of infection. A total of 1655 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pat...
Source: Marine Biotechnology - September 11, 2023 Category: Biotechnology Authors: Jose Priya T A Charutha Karunakaran Aishwarya Nath Sudha Kappalli Source Type: research
Transcriptomic Variation of Amphiprion Percula (Lacep ède, 1802) in Response to Infection with Cryptocaryon Irritans Brown, 1951
Mar Biotechnol (NY). 2023 Sep 11. doi: 10.1007/s10126-023-10246-z. Online ahead of print.ABSTRACTCryptocaryon irritans (Brown 1951) frequently infect the Pomacentridae fishes causing severe economic losses. However, the anti-C. irritans' molecular mechanism in these fishes remains largely unknown. To address this issue, we conducted RNA-Seq for C. irrtians-infected gills of the clownfish Amphiprion percula (Lacepède 1802) at the early (day 1) and late (day 3) stages of infection. A total of 1655 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pat...
Source: Marine Biotechnology - September 11, 2023 Category: Biotechnology Authors: Jose Priya T A Charutha Karunakaran Aishwarya Nath Sudha Kappalli Source Type: research
Transcriptomic Variation of Amphiprion Percula (Lacep ède, 1802) in Response to Infection with Cryptocaryon Irritans Brown, 1951
Mar Biotechnol (NY). 2023 Sep 11. doi: 10.1007/s10126-023-10246-z. Online ahead of print.ABSTRACTCryptocaryon irritans (Brown 1951) frequently infect the Pomacentridae fishes causing severe economic losses. However, the anti-C. irritans' molecular mechanism in these fishes remains largely unknown. To address this issue, we conducted RNA-Seq for C. irrtians-infected gills of the clownfish Amphiprion percula (Lacepède 1802) at the early (day 1) and late (day 3) stages of infection. A total of 1655 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pat...
Source: Marine Biotechnology - September 11, 2023 Category: Biotechnology Authors: Jose Priya T A Charutha Karunakaran Aishwarya Nath Sudha Kappalli Source Type: research
Transcriptomic Variation of Amphiprion Percula (Lacep ède, 1802) in Response to Infection with Cryptocaryon Irritans Brown, 1951
Mar Biotechnol (NY). 2023 Sep 11. doi: 10.1007/s10126-023-10246-z. Online ahead of print.ABSTRACTCryptocaryon irritans (Brown 1951) frequently infect the Pomacentridae fishes causing severe economic losses. However, the anti-C. irritans' molecular mechanism in these fishes remains largely unknown. To address this issue, we conducted RNA-Seq for C. irrtians-infected gills of the clownfish Amphiprion percula (Lacepède 1802) at the early (day 1) and late (day 3) stages of infection. A total of 1655 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pat...
Source: Marine Biotechnology - September 11, 2023 Category: Biotechnology Authors: Jose Priya T A Charutha Karunakaran Aishwarya Nath Sudha Kappalli Source Type: research
Transcriptomic Variation of Amphiprion Percula (Lacep ède, 1802) in Response to Infection with Cryptocaryon Irritans Brown, 1951
Mar Biotechnol (NY). 2023 Sep 11. doi: 10.1007/s10126-023-10246-z. Online ahead of print.ABSTRACTCryptocaryon irritans (Brown 1951) frequently infect the Pomacentridae fishes causing severe economic losses. However, the anti-C. irritans' molecular mechanism in these fishes remains largely unknown. To address this issue, we conducted RNA-Seq for C. irrtians-infected gills of the clownfish Amphiprion percula (Lacepède 1802) at the early (day 1) and late (day 3) stages of infection. A total of 1655 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pat...
Source: Marine Biotechnology - September 11, 2023 Category: Biotechnology Authors: Jose Priya T A Charutha Karunakaran Aishwarya Nath Sudha Kappalli Source Type: research
Transcriptomic Variation of Amphiprion Percula (Lacep ède, 1802) in Response to Infection with Cryptocaryon Irritans Brown, 1951
Mar Biotechnol (NY). 2023 Sep 11. doi: 10.1007/s10126-023-10246-z. Online ahead of print.ABSTRACTCryptocaryon irritans (Brown 1951) frequently infect the Pomacentridae fishes causing severe economic losses. However, the anti-C. irritans' molecular mechanism in these fishes remains largely unknown. To address this issue, we conducted RNA-Seq for C. irrtians-infected gills of the clownfish Amphiprion percula (Lacepède 1802) at the early (day 1) and late (day 3) stages of infection. A total of 1655 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pat...
Source: Marine Biotechnology - September 11, 2023 Category: Biotechnology Authors: Jose Priya T A Charutha Karunakaran Aishwarya Nath Sudha Kappalli Source Type: research
Transcriptomic Variation of Amphiprion Percula (Lacep ède, 1802) in Response to Infection with Cryptocaryon Irritans Brown, 1951
Mar Biotechnol (NY). 2023 Sep 11. doi: 10.1007/s10126-023-10246-z. Online ahead of print.ABSTRACTCryptocaryon irritans (Brown 1951) frequently infect the Pomacentridae fishes causing severe economic losses. However, the anti-C. irritans' molecular mechanism in these fishes remains largely unknown. To address this issue, we conducted RNA-Seq for C. irrtians-infected gills of the clownfish Amphiprion percula (Lacepède 1802) at the early (day 1) and late (day 3) stages of infection. A total of 1655 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pat...
Source: Marine Biotechnology - September 11, 2023 Category: Biotechnology Authors: Jose Priya T A Charutha Karunakaran Aishwarya Nath Sudha Kappalli Source Type: research
Transcriptomic Variation of Amphiprion Percula (Lacep ède, 1802) in Response to Infection with Cryptocaryon Irritans Brown, 1951
Mar Biotechnol (NY). 2023 Sep 11. doi: 10.1007/s10126-023-10246-z. Online ahead of print.ABSTRACTCryptocaryon irritans (Brown 1951) frequently infect the Pomacentridae fishes causing severe economic losses. However, the anti-C. irritans' molecular mechanism in these fishes remains largely unknown. To address this issue, we conducted RNA-Seq for C. irrtians-infected gills of the clownfish Amphiprion percula (Lacepède 1802) at the early (day 1) and late (day 3) stages of infection. A total of 1655 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pat...
Source: Marine Biotechnology - September 11, 2023 Category: Biotechnology Authors: Jose Priya T A Charutha Karunakaran Aishwarya Nath Sudha Kappalli Source Type: research
Transcriptomic Variation of Amphiprion Percula (Lacep ède, 1802) in Response to Infection with Cryptocaryon Irritans Brown, 1951
Mar Biotechnol (NY). 2023 Sep 11. doi: 10.1007/s10126-023-10246-z. Online ahead of print.ABSTRACTCryptocaryon irritans (Brown 1951) frequently infect the Pomacentridae fishes causing severe economic losses. However, the anti-C. irritans' molecular mechanism in these fishes remains largely unknown. To address this issue, we conducted RNA-Seq for C. irrtians-infected gills of the clownfish Amphiprion percula (Lacepède 1802) at the early (day 1) and late (day 3) stages of infection. A total of 1655 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pat...
Source: Marine Biotechnology - September 11, 2023 Category: Biotechnology Authors: Jose Priya T A Charutha Karunakaran Aishwarya Nath Sudha Kappalli Source Type: research
Tetrodotoxins in Larval Development of Ribbon Worm Cephalothrix cf. simula (Palaeonemertea, Nemertea)
Mar Biotechnol (NY). 2023 Sep 6. doi: 10.1007/s10126-023-10249-w. Online ahead of print.ABSTRACTThe toxic ribbon worm, Cephalothrix cf. simula (Palaeonemertea, Nemertea), possesses extremely high concentrations of tetrodotoxin (TTX). Although TTX has been found in the eggs of this species, the fate of the toxin in the ontogeny of the animal has not been explored. Here, using high performance liquid chromatography with tandem mass spectrometry and immunohistochemistry with anti-TTX antibodies, we examined levels, profile, and localization of TTX and its analogues (TTXs) in larvae of C. cf. simula throughout 41 days post-fer...
Source: Marine Biotechnology - September 6, 2023 Category: Biotechnology Authors: Grigorii V Malykin Peter V Velansky Daria I Melnikova Timur Yu Magarlamov Source Type: research
Tetrodotoxins in Larval Development of Ribbon Worm Cephalothrix cf. simula (Palaeonemertea, Nemertea)
Mar Biotechnol (NY). 2023 Sep 6. doi: 10.1007/s10126-023-10249-w. Online ahead of print.ABSTRACTThe toxic ribbon worm, Cephalothrix cf. simula (Palaeonemertea, Nemertea), possesses extremely high concentrations of tetrodotoxin (TTX). Although TTX has been found in the eggs of this species, the fate of the toxin in the ontogeny of the animal has not been explored. Here, using high performance liquid chromatography with tandem mass spectrometry and immunohistochemistry with anti-TTX antibodies, we examined levels, profile, and localization of TTX and its analogues (TTXs) in larvae of C. cf. simula throughout 41 days post-fer...
Source: Marine Biotechnology - September 6, 2023 Category: Biotechnology Authors: Grigorii V Malykin Peter V Velansky Daria I Melnikova Timur Yu Magarlamov Source Type: research
Tetrodotoxins in Larval Development of Ribbon Worm Cephalothrix cf. simula (Palaeonemertea, Nemertea)
Mar Biotechnol (NY). 2023 Sep 6. doi: 10.1007/s10126-023-10249-w. Online ahead of print.ABSTRACTThe toxic ribbon worm, Cephalothrix cf. simula (Palaeonemertea, Nemertea), possesses extremely high concentrations of tetrodotoxin (TTX). Although TTX has been found in the eggs of this species, the fate of the toxin in the ontogeny of the animal has not been explored. Here, using high performance liquid chromatography with tandem mass spectrometry and immunohistochemistry with anti-TTX antibodies, we examined levels, profile, and localization of TTX and its analogues (TTXs) in larvae of C. cf. simula throughout 41 days post-fer...
Source: Marine Biotechnology - September 6, 2023 Category: Biotechnology Authors: Grigorii V Malykin Peter V Velansky Daria I Melnikova Timur Yu Magarlamov Source Type: research
