Assessing the Effectiveness of Sex-Linked Molecular Markers to Identify Neomale Breeders for the Production of All-Female Progenies of Rainbow Trout
Mar Biotechnol (NY). 2024 Feb;26(1):199-204. doi: 10.1007/s10126-024-10288-x. Epub 2024 Jan 16.ABSTRACTA cultured stock of masculinized rainbow trout was diagnosed with Y-linked markers (sdY and OmyY1) aiming to detect neomales before their use at the production level. To achieve a reliable diagnosis, the following steps were considered: (1) PCR amplification of the housekeeping β-actin gene to determine the DNA quality of samples, (2) validation of the Y-linked markers by their PCR amplification in male and female samples with known sex, and (3) molecular sexing of the masculinized juveniles based on male-specific (XY ge...
Source: Marine Biotechnology - January 16, 2024 Category: Biotechnology Authors: Nelson Colihueque Margarita Parraguez Source Type: research
Conjugated Linoleic Acid Reduces Lipid Accumulation via Down-regulation Expression of Lipogenic Genes and Up-regulation of Apoptotic Genes in Grass Carp (Ctenopharyngodon idella) Adipocyte In Vitro
Mar Biotechnol (NY). 2024 Feb;26(1):169-180. doi: 10.1007/s10126-024-10286-z. Epub 2024 Jan 15.ABSTRACTThe relationship between conjugated linoleic acid (CLA) and lipogenesis has been extensively studied in mammals and some cell lines, but it is relatively rare in fish, and the potential mechanism of action of CLA reducing fat mass remains unclear. The established primary culture model for studying lipogenesis in grass carp (Ctenopharyngodon idella) preadipocytes was used in the present study, and the objective was to explore the effects of CLA on intracellular lipid and TG content, fatty acid composition, and mRNA levels ...
Source: Marine Biotechnology - January 15, 2024 Category: Biotechnology Authors: Hua Yu Zhao-Xia Zou Wei Wei Ying Li Source Type: research
Comparative Transcriptome Analysis Reveals the Role of Ribosome Reduction in Impeding Oogenesis in Female Triploid Crassostrea Gigas
This study offers valuable insights into the oogenesis process in female triploid C. gigas.PMID:38217752 | DOI:10.1007/s10126-024-10283-2 (Source: Marine Biotechnology)
Source: Marine Biotechnology - January 13, 2024 Category: Biotechnology Authors: Qiong Yang Hong Yu Qi Li Source Type: research
Alternative Splicing Reveals Acute Stress Response of Litopenaeus vannamei at High Alkalinity
Mar Biotechnol (NY). 2024 Feb;26(1):103-115. doi: 10.1007/s10126-023-10281-w. Epub 2024 Jan 11.ABSTRACTAlkalinity is regarded as one of the primary stressors for aquatic animals in saline-alkaline water. Alternative splicing (AS) can significantly increase the diversity of transcripts and play key roles in stress response; however, the studies on AS under alkalinity stress of crustaceans are still limited. In the present study, we devoted ourselves to the study of AS under acute alkalinity stress at control (50 mg/L) and treatment groups (350 mg/L) by RNA-seq in pacific white shrimp (Litopenaeus vannamei). We identified a ...
Source: Marine Biotechnology - January 11, 2024 Category: Biotechnology Authors: Xiang Shi Ruiqi Zhang Zhe Liu Guiyan Zhao Jintao Guo Xue Mao Baoyi Fan Source Type: research
Genetic Modification of Aurantiochytrium sp. 18W-13a for Enhancement of Proteolytic Activity by Heterologous Expression of Extracellular Proteases
Mar Biotechnol (NY). 2024 Jan 5. doi: 10.1007/s10126-023-10280-x. Online ahead of print.ABSTRACTA marine thraustochytrid, Aurantiochytrium, is a promising organism to produce docosahexaenoic acid (DHA) and squalene. Utilization of inexpensive substances such as proteins in wastes and by-products from the food industry for cultivation is a considerable option to reduce production cost; however, the proteolytic ability of Aurantiochytrium spp. is low compared to taxonomically close Shizochytrium aggregatum. We previously identified extracellular protease (extracellular protease 1, EP1) in S. aggregatum ATCC 28209 from the su...
Source: Marine Biotechnology - January 5, 2024 Category: Biotechnology Authors: Kohei Yoneda Chun Hung Man Yoshiaki Maeda Iwane Suzuki Source Type: research
Genetic Modification of Aurantiochytrium sp. 18W-13a for Enhancement of Proteolytic Activity by Heterologous Expression of Extracellular Proteases
Mar Biotechnol (NY). 2024 Jan 5. doi: 10.1007/s10126-023-10280-x. Online ahead of print.ABSTRACTA marine thraustochytrid, Aurantiochytrium, is a promising organism to produce docosahexaenoic acid (DHA) and squalene. Utilization of inexpensive substances such as proteins in wastes and by-products from the food industry for cultivation is a considerable option to reduce production cost; however, the proteolytic ability of Aurantiochytrium spp. is low compared to taxonomically close Shizochytrium aggregatum. We previously identified extracellular protease (extracellular protease 1, EP1) in S. aggregatum ATCC 28209 from the su...
Source: Marine Biotechnology - January 5, 2024 Category: Biotechnology Authors: Kohei Yoneda Chun Hung Man Yoshiaki Maeda Iwane Suzuki Source Type: research
Genetic Modification of Aurantiochytrium sp. 18W-13a for Enhancement of Proteolytic Activity by Heterologous Expression of Extracellular Proteases
Mar Biotechnol (NY). 2024 Jan 5. doi: 10.1007/s10126-023-10280-x. Online ahead of print.ABSTRACTA marine thraustochytrid, Aurantiochytrium, is a promising organism to produce docosahexaenoic acid (DHA) and squalene. Utilization of inexpensive substances such as proteins in wastes and by-products from the food industry for cultivation is a considerable option to reduce production cost; however, the proteolytic ability of Aurantiochytrium spp. is low compared to taxonomically close Shizochytrium aggregatum. We previously identified extracellular protease (extracellular protease 1, EP1) in S. aggregatum ATCC 28209 from the su...
Source: Marine Biotechnology - January 5, 2024 Category: Biotechnology Authors: Kohei Yoneda Chun Hung Man Yoshiaki Maeda Iwane Suzuki Source Type: research
Genetic Modification of Aurantiochytrium sp. 18W-13a for Enhancement of Proteolytic Activity by Heterologous Expression of Extracellular Proteases
Mar Biotechnol (NY). 2024 Jan 5. doi: 10.1007/s10126-023-10280-x. Online ahead of print.ABSTRACTA marine thraustochytrid, Aurantiochytrium, is a promising organism to produce docosahexaenoic acid (DHA) and squalene. Utilization of inexpensive substances such as proteins in wastes and by-products from the food industry for cultivation is a considerable option to reduce production cost; however, the proteolytic ability of Aurantiochytrium spp. is low compared to taxonomically close Shizochytrium aggregatum. We previously identified extracellular protease (extracellular protease 1, EP1) in S. aggregatum ATCC 28209 from the su...
Source: Marine Biotechnology - January 5, 2024 Category: Biotechnology Authors: Kohei Yoneda Chun Hung Man Yoshiaki Maeda Iwane Suzuki Source Type: research
Genetic Modification of Aurantiochytrium sp. 18W-13a for Enhancement of Proteolytic Activity by Heterologous Expression of Extracellular Proteases
Mar Biotechnol (NY). 2024 Jan 5. doi: 10.1007/s10126-023-10280-x. Online ahead of print.ABSTRACTA marine thraustochytrid, Aurantiochytrium, is a promising organism to produce docosahexaenoic acid (DHA) and squalene. Utilization of inexpensive substances such as proteins in wastes and by-products from the food industry for cultivation is a considerable option to reduce production cost; however, the proteolytic ability of Aurantiochytrium spp. is low compared to taxonomically close Shizochytrium aggregatum. We previously identified extracellular protease (extracellular protease 1, EP1) in S. aggregatum ATCC 28209 from the su...
Source: Marine Biotechnology - January 5, 2024 Category: Biotechnology Authors: Kohei Yoneda Chun Hung Man Yoshiaki Maeda Iwane Suzuki Source Type: research
Rapid Visual Detection of Spiroplasma eriocheiris by Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye
Mar Biotechnol (NY). 2024 Jan 3. doi: 10.1007/s10126-023-10282-9. Online ahead of print.ABSTRACTIn recent years, a new type of Spiroplasma has been found that can cause "tremor disease" of the Chinese mitten crab Eriocheir sinensis. The outbreak of epidemic tremor disease has caused a serious setback in the Chinese mitten crab farming industry, with an incidence rate of more than 30% and mortality rates of 80-100%. Therefore, finding a sensitive method to detect tremor disease in E. sinensis has become a current research focus. In this research, a loop-mediated isothermal amplification detection method coupled with hydroxy...
Source: Marine Biotechnology - January 3, 2024 Category: Biotechnology Authors: Shun Zhou Zongrui Yang Baoshan Guo Jingyuan Yi Yunfei Pang Ruixin Feng Jiaxue Song Yunji Xiu Source Type: research
Rapid Visual Detection of Spiroplasma eriocheiris by Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye
Mar Biotechnol (NY). 2024 Jan 3. doi: 10.1007/s10126-023-10282-9. Online ahead of print.ABSTRACTIn recent years, a new type of Spiroplasma has been found that can cause "tremor disease" of the Chinese mitten crab Eriocheir sinensis. The outbreak of epidemic tremor disease has caused a serious setback in the Chinese mitten crab farming industry, with an incidence rate of more than 30% and mortality rates of 80-100%. Therefore, finding a sensitive method to detect tremor disease in E. sinensis has become a current research focus. In this research, a loop-mediated isothermal amplification detection method coupled with hydroxy...
Source: Marine Biotechnology - January 3, 2024 Category: Biotechnology Authors: Shun Zhou Zongrui Yang Baoshan Guo Jingyuan Yi Yunfei Pang Ruixin Feng Jiaxue Song Yunji Xiu Source Type: research
Stochastic Assembly Increases the Complexity and Stability of Shrimp Gut Microbiota During Aquaculture Progression
This study showed substantial variations in shrimp gut microbiota during aquaculture progression and explored the underlying mechanisms regulating these changes.PMID:38165637 | DOI:10.1007/s10126-023-10279-4 (Source: Marine Biotechnology)
Source: Marine Biotechnology - January 2, 2024 Category: Biotechnology Authors: Saisai Zhang Shuang Liu Hongwei Liu Hui Li Jun Luo Aili Zhang Yinpeng Ding Tongjun Ren Wenbo Chen Source Type: research
Effects of Brown Fishmeal on Growth Performance, Digestibility, and Lipid Metabolism of the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)
In this study, the addition of 20% white fishmeal and 35% brown fishmeal to the diet of Chinese soft-shelled turtles did not adversely affect growth performance. Therefore, 20% white fishmeal and 35% brown fishmeal are the most practical feed formulations for Chinese soft-shelled turtles in this study.PMID:38165638 | DOI:10.1007/s10126-023-10274-9 (Source: Marine Biotechnology)
Source: Marine Biotechnology - January 2, 2024 Category: Biotechnology Authors: Guo-Fang Zhong Liang-Fa Zhang Yi Zhuang Qi Li He Huang Cong Cao Zhan-Ying Zhu Zhong-Yuan Huang Nu-An Wang Kun Yuan Source Type: research
Stochastic Assembly Increases the Complexity and Stability of Shrimp Gut Microbiota During Aquaculture Progression
This study showed substantial variations in shrimp gut microbiota during aquaculture progression and explored the underlying mechanisms regulating these changes.PMID:38165637 | DOI:10.1007/s10126-023-10279-4 (Source: Marine Biotechnology)
Source: Marine Biotechnology - January 2, 2024 Category: Biotechnology Authors: Saisai Zhang Shuang Liu Hongwei Liu Hui Li Jun Luo Aili Zhang Yinpeng Ding Tongjun Ren Wenbo Chen Source Type: research
Effects of Brown Fishmeal on Growth Performance, Digestibility, and Lipid Metabolism of the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)
In this study, the addition of 20% white fishmeal and 35% brown fishmeal to the diet of Chinese soft-shelled turtles did not adversely affect growth performance. Therefore, 20% white fishmeal and 35% brown fishmeal are the most practical feed formulations for Chinese soft-shelled turtles in this study.PMID:38165638 | DOI:10.1007/s10126-023-10274-9 (Source: Marine Biotechnology)
Source: Marine Biotechnology - January 2, 2024 Category: Biotechnology Authors: Guo-Fang Zhong Liang-Fa Zhang Yi Zhuang Qi Li He Huang Cong Cao Zhan-Ying Zhu Zhong-Yuan Huang Nu-An Wang Kun Yuan Source Type: research
