Transcriptomic analysis reveals the inhibitory effect of beta-sitosterol on proliferation of bovine preadipocytes
This study, based on RNA-seq, reveals the impact of Beta -sitosterol on the proliferation of bovine preadipocytes. Compared to the control group, Beta-sitosterol demonstrated a more pronounced inhibitory effect on cell proliferation after 48 hours of treatment than after 24 hours, as evidenced by the results of EdU staining and flow cytometry. RNA-seq and Western Blot analyses further substantiated these findings. Our results suggest that the impact of Beta-sitosterol on the proliferation of bovine preadipocytes is not significant after a 24-hour treatment. It is only after extending the treatment time to 48 hours that Bet...
Source: Animal Biotechnology - April 18, 2024 Category: Biotechnology Authors: Lei Jiang Yuan Wan Jinhai Pan Xiaoyu Mao Xiaolei Sun Linsen Zan Hongbao Wang Source Type: research
MiR-2284b regulation of α-s1 casein synthesis in mammary epithelial cells of dairy goats
Anim Biotechnol. 2024 Nov;35(1):2334725. doi: 10.1080/10495398.2024.2334725. Epub 2024 Apr 16.ABSTRACTThe lactation character of dairy goats is the most important characteristic, and milk protein is an important index to evaluate milk quality. Casein accounts for more than 80% of the total milk protein in goat milk and is the main component of milk protein. Using GMECs (goat mammary epithelial cells) as the research object, the CHECK2 vector of the CSN1S1 gene and the overexpression vector of pcDNA 3.1 were constructed, and the mimics of miR-2284b and the interfering RNA of CSN1S1 were synthesized. Using PCR, RT-qPCR, a du...
Source: Animal Biotechnology - April 16, 2024 Category: Biotechnology Authors: Jinxing Hou Wenfei Li Xiaolong Xu Ao Sun Ganggang Xu Zefang Cheng Haoyuan Zhang Xiaopeng An Source Type: research
MiR-2284b regulation of α-s1 casein synthesis in mammary epithelial cells of dairy goats
Anim Biotechnol. 2024 Nov;35(1):2334725. doi: 10.1080/10495398.2024.2334725. Epub 2024 Apr 16.ABSTRACTThe lactation character of dairy goats is the most important characteristic, and milk protein is an important index to evaluate milk quality. Casein accounts for more than 80% of the total milk protein in goat milk and is the main component of milk protein. Using GMECs (goat mammary epithelial cells) as the research object, the CHECK2 vector of the CSN1S1 gene and the overexpression vector of pcDNA 3.1 were constructed, and the mimics of miR-2284b and the interfering RNA of CSN1S1 were synthesized. Using PCR, RT-qPCR, a du...
Source: Animal Biotechnology - April 16, 2024 Category: Biotechnology Authors: Jinxing Hou Wenfei Li Xiaolong Xu Ao Sun Ganggang Xu Zefang Cheng Haoyuan Zhang Xiaopeng An Source Type: research
Genetic variant of the sheep < em > E2F8 < /em > gene and its associations with litter size
Anim Biotechnol. 2024 Nov;35(1):2337751. doi: 10.1080/10495398.2024.2337751. Epub 2024 Apr 10.ABSTRACTThe economic efficiency of sheep breeding, aiming to enhance productivity, is a focal point for improvement of sheep breeding. Recent studies highlight the involvement of the Early Region 2 Binding Factor transcription factor 8 (E2F8) gene in female reproduction. Our group's recent genome-wide association study (GWAS) emphasizes the potential impact of the E2F8 gene on prolificacy traits in Australian White sheep (AUW). Herein, the purpose of this study was to assess the correlation of the E2F8 gene with litter size in AUW...
Source: Animal Biotechnology - April 10, 2024 Category: Biotechnology Authors: Leijing Zhu Nazar Akhmet Didi Bo Chuanying Pan Jiyao Wu Xianyong Lan Source Type: research
Genetic variant of the sheep < em > E2F8 < /em > gene and its associations with litter size
Anim Biotechnol. 2024 Nov;35(1):2337751. doi: 10.1080/10495398.2024.2337751. Epub 2024 Apr 10.ABSTRACTThe economic efficiency of sheep breeding, aiming to enhance productivity, is a focal point for improvement of sheep breeding. Recent studies highlight the involvement of the Early Region 2 Binding Factor transcription factor 8 (E2F8) gene in female reproduction. Our group's recent genome-wide association study (GWAS) emphasizes the potential impact of the E2F8 gene on prolificacy traits in Australian White sheep (AUW). Herein, the purpose of this study was to assess the correlation of the E2F8 gene with litter size in AUW...
Source: Animal Biotechnology - April 10, 2024 Category: Biotechnology Authors: Leijing Zhu Nazar Akhmet Didi Bo Chuanying Pan Jiyao Wu Xianyong Lan Source Type: research
Genetic variant of the sheep < em > E2F8 < /em > gene and its associations with litter size
Anim Biotechnol. 2024 Nov;35(1):2337751. doi: 10.1080/10495398.2024.2337751. Epub 2024 Apr 10.ABSTRACTThe economic efficiency of sheep breeding, aiming to enhance productivity, is a focal point for improvement of sheep breeding. Recent studies highlight the involvement of the Early Region 2 Binding Factor transcription factor 8 (E2F8) gene in female reproduction. Our group's recent genome-wide association study (GWAS) emphasizes the potential impact of the E2F8 gene on prolificacy traits in Australian White sheep (AUW). Herein, the purpose of this study was to assess the correlation of the E2F8 gene with litter size in AUW...
Source: Animal Biotechnology - April 10, 2024 Category: Biotechnology Authors: Leijing Zhu Nazar Akhmet Didi Bo Chuanying Pan Jiyao Wu Xianyong Lan Source Type: research
Genetic variant of the sheep < em > E2F8 < /em > gene and its associations with litter size
Anim Biotechnol. 2024 Nov;35(1):2337751. doi: 10.1080/10495398.2024.2337751. Epub 2024 Apr 10.ABSTRACTThe economic efficiency of sheep breeding, aiming to enhance productivity, is a focal point for improvement of sheep breeding. Recent studies highlight the involvement of the Early Region 2 Binding Factor transcription factor 8 (E2F8) gene in female reproduction. Our group's recent genome-wide association study (GWAS) emphasizes the potential impact of the E2F8 gene on prolificacy traits in Australian White sheep (AUW). Herein, the purpose of this study was to assess the correlation of the E2F8 gene with litter size in AUW...
Source: Animal Biotechnology - April 10, 2024 Category: Biotechnology Authors: Leijing Zhu Nazar Akhmet Didi Bo Chuanying Pan Jiyao Wu Xianyong Lan Source Type: research
Exploring rumen fermentation and microbial populations in Dhofari goats fed a chitosan-added diet
The objective of this study was to explore the rumen fermentation and microbial populations in Dhofari goats fed a diet supplemented with CHI. A total of 24 Dhofari lactating goats (body weight, 27.32 ± 1.80 kg) were assigned randomly into three experimental groups (n = 8 ewes/group). Goats were fed a basal diet with either 0 (control), 180 (low), or 360 (high) mg CHI/kg of dietary dry matter (DM) for 45 days. Feeding high CHI linearly increased (p < 0.05) the propionate level and reduced the acetate, butyrate, and total protozoa count (p < 0.05). Ruminal ammonia nitrogen (NH3-N) concentrations and the acetate:propi...
Source: Animal Biotechnology - April 9, 2024 Category: Biotechnology Authors: Hani M El-Zaiat Waleed Al-Marzooqi Kaadhia Al-Kharousi Source Type: research
Exploring rumen fermentation and microbial populations in Dhofari goats fed a chitosan-added diet
The objective of this study was to explore the rumen fermentation and microbial populations in Dhofari goats fed a diet supplemented with CHI. A total of 24 Dhofari lactating goats (body weight, 27.32 ± 1.80 kg) were assigned randomly into three experimental groups (n = 8 ewes/group). Goats were fed a basal diet with either 0 (control), 180 (low), or 360 (high) mg CHI/kg of dietary dry matter (DM) for 45 days. Feeding high CHI linearly increased (p < 0.05) the propionate level and reduced the acetate, butyrate, and total protozoa count (p < 0.05). Ruminal ammonia nitrogen (NH3-N) concentrations and the acetate:propi...
Source: Animal Biotechnology - April 9, 2024 Category: Biotechnology Authors: Hani M El-Zaiat Waleed Al-Marzooqi Kaadhia Al-Kharousi Source Type: research
Vitamin B5 supplementation enhances intestinal development and alters microbes in weaned piglets
This study explored the effects of different vitamin B5 (VB5) levels on intestinal growth and function of weaned piglets. Twenty-one piglets (7.20 ± 1.11 kg) were included in a 28-day feeding trial with three treatments, including 0 mg/kg (L-VB5), 10 mg/kg (Control) and 50 mg/kg (H-VB5) of VB5 supplement. The results showed that: Large intestine weight/body weight was the highest in H-VB5 group, Control and H-VB5 groups had significantly higher villus height and villus height/crypt depth than the L-VB5 in the ileum (p < .05). Goblet cells (ileal crypt) and endocrine cells (ileal villus) significantly increased in Contr...
Source: Animal Biotechnology - April 8, 2024 Category: Biotechnology Authors: Xin Wang Yan Qin Jianzhong Li Pengfei Huang Yali Li Jing Huang Qiye Wang Huansheng Yang Source Type: research
Vitamin B5 supplementation enhances intestinal development and alters microbes in weaned piglets
This study explored the effects of different vitamin B5 (VB5) levels on intestinal growth and function of weaned piglets. Twenty-one piglets (7.20 ± 1.11 kg) were included in a 28-day feeding trial with three treatments, including 0 mg/kg (L-VB5), 10 mg/kg (Control) and 50 mg/kg (H-VB5) of VB5 supplement. The results showed that: Large intestine weight/body weight was the highest in H-VB5 group, Control and H-VB5 groups had significantly higher villus height and villus height/crypt depth than the L-VB5 in the ileum (p < .05). Goblet cells (ileal crypt) and endocrine cells (ileal villus) significantly increased in Contr...
Source: Animal Biotechnology - April 8, 2024 Category: Biotechnology Authors: Xin Wang Yan Qin Jianzhong Li Pengfei Huang Yali Li Jing Huang Qiye Wang Huansheng Yang Source Type: research
Effects of dietary supplementation of grape seed extract in comparison with excessive level of vitamin E on growth performance and antioxidant function of broilers
Anim Biotechnol. 2024 Nov;35(1):2331640. doi: 10.1080/10495398.2024.2331640. Epub 2024 Mar 25.ABSTRACTThe present study was carried out to evaluate the effects of dietary vitamin E (VE) or grape seed extract (GSE) on the growth performance and antioxidant function of broilers. Two hundred sixteen broiler chicks were randomly assigned to 3 diets: diet supplemented with oxidized rice bran oil (CN group), CN group with 25 mg/kg VE or 100 mg/kg GSE. Dietary VE or GSE improved the growth performance, reverted the disturbed levels of liver antioxidant enzymes, and reduced liver damage of broilers fed oxidized rice bran oil. The ...
Source: Animal Biotechnology - March 25, 2024 Category: Biotechnology Authors: Man Wang Zongze He Zhaolong Xiong Hongwei Liu Xiang Zhou Jian He Source Type: research
Effects of dietary supplementation of grape seed extract in comparison with excessive level of vitamin E on growth performance and antioxidant function of broilers
Anim Biotechnol. 2024 Nov;35(1):2331640. doi: 10.1080/10495398.2024.2331640. Epub 2024 Mar 25.ABSTRACTThe present study was carried out to evaluate the effects of dietary vitamin E (VE) or grape seed extract (GSE) on the growth performance and antioxidant function of broilers. Two hundred sixteen broiler chicks were randomly assigned to 3 diets: diet supplemented with oxidized rice bran oil (CN group), CN group with 25 mg/kg VE or 100 mg/kg GSE. Dietary VE or GSE improved the growth performance, reverted the disturbed levels of liver antioxidant enzymes, and reduced liver damage of broilers fed oxidized rice bran oil. The ...
Source: Animal Biotechnology - March 25, 2024 Category: Biotechnology Authors: Man Wang Zongze He Zhaolong Xiong Hongwei Liu Xiang Zhou Jian He Source Type: research
Effects of dietary supplementation of grape seed extract in comparison with excessive level of vitamin E on growth performance and antioxidant function of broilers
Anim Biotechnol. 2024 Nov;35(1):2331640. doi: 10.1080/10495398.2024.2331640. Epub 2024 Mar 25.ABSTRACTThe present study was carried out to evaluate the effects of dietary vitamin E (VE) or grape seed extract (GSE) on the growth performance and antioxidant function of broilers. Two hundred sixteen broiler chicks were randomly assigned to 3 diets: diet supplemented with oxidized rice bran oil (CN group), CN group with 25 mg/kg VE or 100 mg/kg GSE. Dietary VE or GSE improved the growth performance, reverted the disturbed levels of liver antioxidant enzymes, and reduced liver damage of broilers fed oxidized rice bran oil. The ...
Source: Animal Biotechnology - March 25, 2024 Category: Biotechnology Authors: Man Wang Zongze He Zhaolong Xiong Hongwei Liu Xiang Zhou Jian He Source Type: research
Effects of dietary supplementation of grape seed extract in comparison with excessive level of vitamin E on growth performance and antioxidant function of broilers
Anim Biotechnol. 2024 Nov;35(1):2331640. doi: 10.1080/10495398.2024.2331640. Epub 2024 Mar 25.ABSTRACTThe present study was carried out to evaluate the effects of dietary vitamin E (VE) or grape seed extract (GSE) on the growth performance and antioxidant function of broilers. Two hundred sixteen broiler chicks were randomly assigned to 3 diets: diet supplemented with oxidized rice bran oil (CN group), CN group with 25 mg/kg VE or 100 mg/kg GSE. Dietary VE or GSE improved the growth performance, reverted the disturbed levels of liver antioxidant enzymes, and reduced liver damage of broilers fed oxidized rice bran oil. The ...
Source: Animal Biotechnology - March 25, 2024 Category: Biotechnology Authors: Man Wang Zongze He Zhaolong Xiong Hongwei Liu Xiang Zhou Jian He Source Type: research
