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Total 393 results found since Jan 2013.

MiR-199a-5p regulates rat liver regeneration and hepatocyte proliferation by targeting TNF- α TNFR1/TRADD/CASPASE8/CASPASE3 signalling pathway.
MiR-199a-5p regulates rat liver regeneration and hepatocyte proliferation by targeting TNF-α TNFR1/TRADD/CASPASE8/CASPASE3 signalling pathway. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):4110-4118 Authors: Zhang C, Ye B, Wei J, Wang Q, Xu C, Yu G Abstract Abnormally expressed miR-199a-5p (miR-199a) has been frequently reported in multiple types of malignancies. Nevertheless, its effect in liver regeneration (LR) is largely still unclear. Herein, we investigated the function of miR-199a in hepatocyte proliferation during LR. As a result, miR-199a expression was significantly increased 12-30 h, in ...
Source: Artificial Cells, Nanomedicine and Biotechnology - November 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

A 1,8-naphthalimide-12aneN3 derivative for efficient Cu2+ recognition, lysosome staining and siRNA delivery.
Abstract Development of multifunctional compounds as both fluorescence probes and non-viral vectors is still difficult till date. It is necessary to overcome many hurdles such as the balance of hydrophilic and hydrophobic moieties, binding affinity between multifunctional compound and targeting substrate, the cytotoxicity of multifunctional compound, and so on. In this work, the performances of compound 1 on Cu2+ recognition, lysosome staining and siRNA (small interfering RNA) delivery were investigated. It was found that compound 1 exhibited high selectivity and sensitivity toward Cu2+ in aqueous solutions. The f...
Source: Colloids and Surfaces - October 31, 2019 Category: Biotechnology Authors: Gao YG, Dang K, Zhang WJ, Liu FL, Patil S, Qadir A, Ding AX, Qian AR Tags: Colloids Surf B Biointerfaces Source Type: research

PLCG2 promotes hepatocyte proliferation in vitro via NF- κB and ERK pathway by targeting bcl2, myc and ccnd1.
In this study, we investigate the mechanism of PLCG2 action using a short interference RNA (siRNA) method. The effects of PLCG2 on rat liver BRL-3A cells treated siRNA were studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT assay), bromodeoxyuridine (BrdU) labelling assay, flow cytometry method (FCM), quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. The results showed when PLCG2 was reduced, cell vitality and proliferation rate were significantly decreased (p < .05 vs. control). FCM analysis showed that the number of cell division phase (G2 + M) was decl...
Source: Artificial Cells, Nanomedicine and Biotechnology - September 26, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Osteopontin promotes rat hepatocyte proliferation both in vitro and in vivo.
Conclusion: OPN promotes hepatocyte proliferation both in vitro and in vivo through STAT3 and AKT signaling pathways. PMID: 31544532 [PubMed - in process]
Source: Artificial Cells, Nanomedicine and Biotechnology - September 25, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Lipid-based Vehicles for siRNA Delivery in Biomedical Field.
Abstract Genetic drugs have aroused much attention in the past twenty years. RNA interference (RNAi) offers novel insights into discovering potential gene functions and therapies targeting genetic diseases. Small interference RNA (siRNA), typically 21-23 nucleotides in length, can specifically degrade complementary mRNA. However, targeted delivery and controlled release of siRNA remain a great challenge. Considering the vast number of drug carriers, when compared with viral vectors, non-viral ones show reduced cytotoxicity and low production cost. Especially, lipid-based vehicles are more promising due to their hi...
Source: Current Pharmaceutical Biotechnology - September 23, 2019 Category: Biotechnology Authors: Li T, Huang L, Yang M Tags: Curr Pharm Biotechnol Source Type: research

Molecular and expression characteristics of resistin (RETN) and its effects on the differentiation of intramuscular preadipocyte in goat.
In this study, the expression of RETN mRNA in goat tissues and intramuscular preadipocytes during differentiation was detected by qPCR technique. Furthermore, RNA interference was used to explore the effects of RETN on intramuscular preadipocytes differentiation in goat. The results showed that the cloned goat RETN gene sequence was 428 bp in length, of which the ORF was 330 bp, encoding 109 amino acids. Sequence analysis revealed that it had 12 phosphorylation sites and an O-glycosylation site, and its protein contained a signal peptide sequence. Also, the RETN gene is expressed in goat various analyzed tissues, and t...
Source: Animal Biotechnology - September 12, 2019 Category: Biotechnology Authors: Zhang Y, Wang Y, Xu Q, Zhu J, Lin Y Tags: Anim Biotechnol Source Type: research

miR-342 suppresses the proliferation and invasion of acute myeloid leukemia by targeting Naa10p.
In conclusion, our study confirmed that miR-342/Naa10p plays key roles in AML progression, providing insights into underlying mechanisms of AML pathogenesis and also a potential therapeutic target for this malignancy. PMID: 31496296 [PubMed - in process]
Source: Artificial Cells, Nanomedicine and Biotechnology - September 11, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Targeting clusterin induces apoptosis, reduces growth ability, invasion and mediates sensitivity to chemotherapy in human cancer in human osteosarcoma cells.
CONCLUSIONS: Overexpression of sCLU was significantly correlated with metastasis and chemosensitivity in osteosarcoma cells. The described sCLU-specific siRNA that can potently silence sCLU gene expression may thus prove valuable agents during antitumor therapy. PMID: 31433751 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Biotechnology - August 20, 2019 Category: Biotechnology Authors: Wang X, Yu Y, Zang L, Zhang P, Ma J, Chen D Tags: Curr Pharm Biotechnol Source Type: research

miR-34a inhibits proliferation, migration and invasion of paediatric neuroblastoma cells via targeting HNF4 α.
Conclusion: The expression of miR-34a was down-regulated in paediatric neuroblastoma tissues, and overexpression of miR-34a could inhibit proliferation, migration and invasion of SH-SY5Y cells by targeting HNF4α. PMID: 31343368 [PubMed - in process]
Source: Artificial Cells, Nanomedicine and Biotechnology - July 27, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Transcriptome profiling of cervical cancer cells acquired resistance to cisplatin by deep sequencing.
In this study, we developed cisplatin resistance cell line, subsequently examined the molecular mechanisms linked. Transcriptome sequencing technology was utilized to compare the various expression models between the cisplatin-resistant cell line (Hela/DDP) and its parental cell line human cervical adenocarcinoma Hela. The present study has identified 2,312 differentially expressed genes (DEGs). Results showed there were 1,437 up-regulated genes and 875 down-regulated ones. Databases analysis including Gene ontology (GO), Cluster of Orthologous Groups of proteins (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pat...
Source: Artificial Cells, Nanomedicine and Biotechnology - July 13, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

FAK mediates BMP9-induced osteogenic differentiation via Wnt and MAPK signaling pathway in synovial mesenchymal stem cells.
Conclusion: FAK plays a pivotal role in promoting BMP9-induced osteogenesis of SMSCs, which is probably via activating Wnt and MAPK pathway. PMID: 31240956 [PubMed - in process]
Source: Artificial Cells, Nanomedicine and Biotechnology - June 27, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Molecular characterization, expression analysis of Chemerin gene and its potential role in intramuscular adipocyte differentiation of goat.
Abstract Chemerin is a cytokine secreted by adipose cells and plays an important regulatory role in adipocyte differentiation and lipid metabolism. However, the function of Chemerin on intramuscular fat (IMF) deposition is still unknown in goat. Here, we cloned the cDNA of goat Chemerin gene using quantitative real-time PCR (qRT-PCR) technique and further clarified the expression pattern of Chemerin in various tissues of goat. The siRNA against Chemerin were synthesized to explore the potential role in intramuscular preadipocyte differentiation of goat. Our results showed that the full-length open reading frame (O...
Source: Animal Biotechnology - June 12, 2019 Category: Biotechnology Authors: Ma J, Liao H, Lin Y, Huang K, Zhu J, Wang Y Tags: Anim Biotechnol Source Type: research

Pretreatment of ghrelin protects H9c2 cells against hypoxia/reoxygenation-induced cell death via PI3K/AKT and AMPK pathways.
In conclusion, this study demonstrated that ghrelin pretreatment protected H9c2 cells against H/R-induced cell damage, possibly via PI3K/AKT and AMPK pathways. PMID: 31159591 [PubMed - in process]
Source: Artificial Cells, Nanomedicine and Biotechnology - June 6, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Cyclin D1 regulates osteoarthritis chondrocyte apoptosis via WNT3/ β-catenin signalling.
Conclusions: Cyclin D1 regulated chondrocyte proliferation and apoptosis through Wnt3/β-catenin instead of Wnt10a/β-catenin signalling pathway. PMID: 31155960 [PubMed - in process]
Source: Artificial Cells, Nanomedicine and Biotechnology - June 4, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Study of As2O3 regulating proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1.
CONCLUSION: As2O3 could regulate the proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1. PMID: 31079498 [PubMed - in process]
Source: Artificial Cells, Nanomedicine and Biotechnology - May 15, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research