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Specialty: Biotechnology
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Total 393 results found since Jan 2013.
The diffusion-driven microfluidic process to manufacture lipid-based nanotherapeutics with stealth properties for siRNA delivery
Colloids Surf B Biointerfaces. 2022 Mar 23;215:112476. doi: 10.1016/j.colsurfb.2022.112476. Online ahead of print.ABSTRACTOur study investigated the manufacturing of lipid-based nanotherapeutics with stealth properties for siRNA delivery by employing a diffusion-driven microfluidic process in one or two-steps strategies to produce siRNA-loaded lipid nanocarriers and lipoplexes, respectively. In the one-step synthesis, siRNA in the aqueous phase is introduced from one inlet, while phospholipids dispersed in anhydrous ethanol are introduced from other inlets, generating the lipid nanocarriers. In the two-steps strategies, th...
Source: Colloids and Surfaces - April 7, 2022 Category: Biotechnology Authors: Ismail E ş Antonio A Malfatti-Gasperini Lucimara Gaziola de la Torre Source Type: research
High transfection efficiency and cell viability of immune cells with nanomaterials-based transfection reagent
Biotechniques. 2022 Apr 4. doi: 10.2144/btn-2022-0024. Online ahead of print.ABSTRACTGene manipulation in non-adhesive cells, especially lymphocytes, was difficult due to their low efficiency and high toxicity. Electroporation was reported as a highly efficient method for human and mouse lymphocytes. However, this method requires expensive equipment and causes severe cell damage. Here, the authors present a simple and efficient method to deliver siRNA into lymphocytes with high efficiency and cell viability. This nanomaterials-based transfection reagent was simple and cost-effective and can perform multiple transfections, ...
Source: BioTechniques - April 4, 2022 Category: Biotechnology Authors: Song Wang Dan Tian Source Type: research
< em > Chelidonium majus < /em > Induces Apoptosis of Human Ovarian Cancer Cells Via ATF3-Mediated Regulation of Foxo3a by Tip60
This study demonstrated that Chelidonium majus upregulated the expression of ATF3 and Tip60 and promoted Foxo3a nuclear translocation, ultimately increasing the level of Bcl-2-associated X protein (Bax) protein. ATF3 overexpression stimulated Tip60 expression, while ATF3 inhibition by siRNA repressed Tip60 expression. Furthermore, siRNA-mediated Tip60 inhibition significantly promoted Foxo3a phosphorylation, leading to blockade of Foxo3a translocation into the nucleus. Thus, we were able to deduce that ATF3 mediates the regulation of Foxo3a by Tip60. Moreover, siRNA-mediated Foxo3a inhibition suppressed the expression of B...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Lei Shen Soon Lee Jong Cheon Joo Eunmi Hong Zhen Yang Cui Eunbi Jo Soo Jung Park Hyun-Jin Jang Source Type: research
