Biologically rapid synthesis of silver nanoparticles by Sphingobium sp. MAH-11T and their antibacterial activity and mechanisms investigation against drug-resistant pathogenic microbes.
Authors: Akter S, Huq MA Abstract The present study highlights the biological synthesis of silver nanoparticles (AgNPs) using Sphingobium sp. MAH-11 and also their antibacterial mechanisms against drug-resistant pathogenic microorganisms. The nanoparticle synthesis method used in this study was reliable, facile, rapid, cost-effective and ecofriendly. The AgNPs exhibited the highest absorbance at 423 nm. The TEM image expressed spherical shape of AgNPs and the size of synthesized AgNPs was 7-22 nm. The selected area diffraction (SAED) pattern and XRD spectrum revealed the crystalline structure of AgNPs...
Source: Artificial Cells, Nanomedicine and Biotechnology - February 22, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Silencing circular RNA-ZNF652 represses proliferation and EMT process of renal carcinoma cells via raising miR-205.
Authors: Zhang L, Guo Y Abstract Recently, the functions of circular RNAs (circRNAs) on cancer initiation and development arouse wide concern. Herein, we tested the influences of circ-ZNF652 on renal carcinoma cell growth and metastasis. Firstly, clinical renal carcinoma tissues and corresponding normal tissues were collected. The circ-ZNF652 expressions were tested. Then, the influences of silencing circ-ZNF652 on renal carcinoma A498 and ACHN cell proliferation, apoptosis and epithelial-mesenchymal transition (EMT) process, as well as Ras/Raf/MEK/ERK and JAK1/STAT3 pathways, were probed. Finally, whether miR-205 ...
Source: Artificial Cells, Nanomedicine and Biotechnology - February 20, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

miR-200b regulates cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysema.
In conclusion, our results suggest that miR-200b may attenuate cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysema. PMID: 32070140 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - February 20, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Long non-coding RNA 91H regulates IGF2 expression by interacting with IGF2BP2 and promotes tumorigenesis in colorectal cancer.
Authors: Gao T, Liu X, He B, Pan Y, Wang S Abstract 91H, a long non-coding antisense transcripts located on the position of the H19/IGF2 locus had been suggested to play a critical role in tumour development. However, little study had proved the mechanism in colorectal cancer (CRC). Hence, we performed this study to deeply explore the mechanism of lncRNA 91H in tumour progression. The expression of lncRNA 91H was first detected in CRC tissues and cells which was higher in vitro and in vivo than normal cells or tissues and CRC patients with high lncRNA 91H expression usually had a high risk in tumour metastasis (p 
Source: Artificial Cells, Nanomedicine and Biotechnology - February 20, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

CD32a polymorphism rs1801274 affects the risk of Kawasaki disease.
Authors: Wang Z, Geng PL Abstract Aim: To analyze the impact of CD32a polymorphism rs1801274 on the occurrence of Kawasaki disease (KD) through the meta-analysis.Methods: The correlation between CD32a polymorphism rs1801274 and the susceptibility to KD was appraised using summarized odds ratios (ORs) with their 95% confidence intervals (95% CIs). Besides, stratification analyses were further implemented on the basis of ethnicity and control source, respectively. Between-study heterogeneity was checked adopting chi-square-based Q test, with p 
Source: Artificial Cells, Nanomedicine and Biotechnology - February 20, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

KANK1 regulates paclitaxel resistance in lung adenocarcinoma A549 cells.
Authors: Pu J, Shen J, Zhong Z, Yanling M, Gao J Abstract Paclitaxel (PTX), a tubulin-binding agent, is widely used and has shown good efficacy in the initial period of treatment for non-small cell lung cancer (NSCLC). However, the relatively rapid acquisition of resistance to PTX treatments that is observed in virtually all cases significantly limits its utility and remains a substantial challenge to the clinical management of NSCLC. The aim of this study was to identify candidate genes and mechanisms that might mediate acquired paclitaxel resistance. In this work, we established paclitaxel-resistant cells (A549-T...
Source: Artificial Cells, Nanomedicine and Biotechnology - February 19, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Protocatechuic aldehyde mitigates hydrogen peroxide-triggered PC12  cell damage by down-regulating MEG3.
Conclusions: The results disclosed the protective impacts of PA on PC12 cells to resist H2O2-provoked damage. MEG3, Wnt/β-catenin and PTEN/PI3K/AKT pathways joined in adjusting the activity of PA in H2O2-damaged PC12 cells. PMID: 32064936 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - February 19, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Stem-like cells of various origins showed therapeutic effect to improve the recovery of spinal cord injury.
Authors: Kang J, Zhang C, Zhi Z, Wang Y, Liu J, Wu F, Xu G Abstract We aimed to evaluate the therapeutic effects of exosomes, which were collected from human neuroepithelial stem cells (HNESCs) treated by miR-29b mimics, on the treatment of spinal cord injury (SCI). Computational analysis, real-time PCR, Western blot analysis and TUNEL assay, a BBB score system, the Nissl staining and IHC assay were conducted to explore the molecular signalling pathway underlying the function of exosomes in SCI. Exosomes isolated from cells treated with HNESC exhibited the strongest inhibitory effect on cell apoptosis while exhibit...
Source: Artificial Cells, Nanomedicine and Biotechnology - February 16, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Long non-coding RNA DDX11-AS1 facilitates gastric cancer progression by regulating miR-873-5p/SPC18 axis.
In conclusion, DDX11-AS1 may serve as an oncogene in GC progression by sponging miR-873-5p and promoting SPC18 expression, providing a new insight into the mechanisms of DDX11-AS1 and elucidating a promising therapy target in GC. PMID: 32054332 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - February 16, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Folic acid-modified celastrol nanoparticles: synthesis, characterization, anticancer activity in 2D and 3D breast cancer models.
Authors: Law S, Leung AW, Xu C Abstract Celastrol is used in traditional Chinese medicine for treating cancers. However, its low water solubility and poor tumour selection represent major pitfalls for clinical application. In the present study, gold nanoparticle (AuNP) firstly was conjugated with PVP-co-2-dimethylaminoethyl methacrylate (Polymer) and celastrol then modified by folic acid. The as-prepared folate receptor-targeted celastrol AuNP (FCA) was characterized using attenuated total reflection Fourier transform infrared spectroscopy, UV-Vis spectrometry, transmission electron microscope, and inductively coup...
Source: Artificial Cells, Nanomedicine and Biotechnology - February 16, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The first and low cost copper Schiff base/manganese oxide bio nanocomposite from unwanted plants as a robust industrial catalyst.
Authors: Naeimi A, Abbasspour S, Torabizadeh SA Abstract In this paper, the first bio-nano colloid including manganese oxide nanoparticles and copper nanocomplex in the presence of Amaranthus spinosus as one unwanted plant was prepared (Mn3O4/CuL bio-nanocolloid). As-prepared bio nanocolloid was analysed completely by different techniques such as FT-IR, ICP-AES, SEM, EDX, TEM and elemental analysis to have the size, structure, morphology and elements in this compound. This bio-nanocolloid showed high catalytic activity towards green oxidation reactions of alcohols using hydrogen peroxide under solvent free conditio...
Source: Artificial Cells, Nanomedicine and Biotechnology - February 14, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Antiviral activity of curcumin-nanoemulsion associated with photodynamic therapy in vulvar cell lines transducing different variants of HPV-16.
Authors: Bonfim CMD, Monteleoni LF, Calmon MF, Cândido NM, Provazzi PJS, Lino VS, Rabachini T, Sichero L, Villa LL, Quintana SM, Melli PPDS, Primo FL, Amantino CF, Tedesco AC, Boccardo E, Rahal P Abstract Vulvar intraepithelial neoplasia (VIN) is associated with human papillomavirus (HPV) infection. Curcumin is a natural bioactive compound with antineoplastic properties. The use of nanoparticles containing curcumin could allow a better performance of this compound in therapies. So, VIN biopsies were collected and HPV DNA detection was performed by PCR, positive samples were genotyped by Restriction Fragment L...
Source: Artificial Cells, Nanomedicine and Biotechnology - February 14, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21.
In conclusion, the neuroprotective effects of MEG3 silence on PC12 cells injured by hypoxia were observed in this study. Mechanistically, the neuroprotective effects of MEG3 silence on PC12 cells were via sponging miR-21 and thus regulating PI3K/AKT and NF-κB pathways.HIGHLIGHTSMEG3 is highly expressed in PC12 cells following hypoxic injury;Silence of MEG3 prevents hypoxia-induced cell damage in PC12 cells;MEG3 acts as a miR-21 sponge;MEG3 sponges miR-21 to regulate PI3K/AKT and NF-κB pathways. PMID: 32050796 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - February 14, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Circular RNA cZNF292 silence alleviates OGD/R-induced injury through up-regulation of miR-22 in rat neural stem cells (NSCs).
Conclusions: The cZNF292 silence alleviated OGD/R-induced injury through the up-regulation of miR-22 in NSCs, and which furnished the theoretical basis for further research on HIE progression. PMID: 32052645 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - February 14, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Knockdown of hsa_circ_0058124 inhibits the proliferation of human lung cancer cells by up-regulation of miR-1297.
Authors: Li X, Zhang Q, Yang Z Abstract Hsa_circ_0058124 was reported to possess the capacity of enhancing tumorigenesis and invasiveness. This investigation explored the effects of hsa_circ_0058124 on human lung cancer. qRT-PCR was employed for assessing the expression of hsa_circ_0058124 and miR-1297. Cell transfection was conducted for altering the expression of hsa_circ_0058124 and miR-1297. Dual-luciferase reporter gene assay was employed for exploring the relationship between hsa_circ_0058124 and miR-1297. CCK-8 assay, colony formation, flow cytometry, western blot and transwell assay were respectively conduc...
Source: Artificial Cells, Nanomedicine and Biotechnology - February 14, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Long non-coding RNA DANCR alleviates hypoxia-caused H9c2 cells damage through up regulation of HIF-1 α.
In conclusion, DANCR alleviated hypoxia-caused H9c2 cells damage through positive regulation of HIF-1α. PMID: 32041436 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - February 13, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The effect of π-Conjugation on the self-assembly of micelles and controlled cargo release.
The effect of π-Conjugation on the self-assembly of micelles and controlled cargo release. Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):525-532 Authors: Liang Y, Sun Y, Fu X, Lin Y, Meng Z, Meng Y, Niu J, Lai Y, Sun Y Abstract Here we presented a novel micelle self-assembled from amphiphiles with π-conjugated moieties (OEG-DPH). The π-conjugated structural integrity of the micelles enabled stable encapsulation of Nile Red (NR, model drug). The self-assembly behaviour of the amphiphiles and the release profile of NR loaded micelles were investigated. Spherical core-shell structured NR loaded ...
Source: Artificial Cells, Nanomedicine and Biotechnology - February 12, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Dexmedetomidine protects PC12 cells from oxidative damage through regulation of miR-199a/HIF-1 α.
Conclusions: This article illustrated the protective effect of Dex on oxidative damage in PC12 cells. Furthermore, Dex prevented PC12 cells from oxidative injury through the regulation of miR-199a/HIF-1α. PMID: 32024386 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - February 7, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Toxicity assessment of superparamagnetic iron oxide nanoparticles in different tissues.
Authors: Vakili-Ghartavol R, Momtazi-Borojeni AA, Vakili-Ghartavol Z, Aiyelabegan HT, Jaafari MR, Rezayat SM, Arbabi Bidgoli S Abstract Superparamagnetic iron oxide nanoparticles (SPIONs) have been employed in several biomedical applications where they facilitate both diagnostic and therapeutic aims. Although the potential benefits of SPIONs with different surface chemistry and conjugated targeting ligands/proteins are considerable, complicated interactions between these nanoparticles (NPs) and cells leading to toxic impacts could limit their clinical applications. Hence, elevation of our knowledge regarding the SP...
Source: Artificial Cells, Nanomedicine and Biotechnology - February 7, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Long non-coding RNA NKILA weakens TNF- α-induced inflammation of MRC-5 cells by miR-21 up-regulation.
Conclusions: NKILA overexpression remitted TNF-α-induced MRC5 cell injury by up-regulation of miR-21 and via inactivation of JNK and NF-κB signaling pathways. PMID: 32013579 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - February 6, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Tanshinone I induces cell apoptosis by reactive oxygen species-mediated endoplasmic reticulum stress and by suppressing p53/DRAM-mediated autophagy in human hepatocellular carcinoma.
Authors: Liu X, Liu J Abstract Human hepatocellular carcinoma (HCC) is the most common type of liver cancer, and it has a high mortality rate. Despite surgical treatments, radiotherapy, and chemotherapy, the median survival of patients with advanced HCC is low. Evidence has shown that tanshinone (TA) I exhibits anti-proliferative activity against numerous cancers. However, the role of TA I and its mechanism in HCC remain unknown. Here, we determined the anti-cancer potential of TA I against HCC cell lines HepG2 and Huh7. Cell viability was analyzed using a Cell Counting Kit-8 assay. Flow cytometry was used to analy...
Source: Artificial Cells, Nanomedicine and Biotechnology - February 6, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Schisandrin B suppresses liver fibrosis in rats by targeting miR-101-5p through the TGF- β signaling pathway.
In conclusion, Sch B can directly target miR-101 to suppress liver fibrosis. Sch B or miR-101-5p may be used as a therapeutic approach for the prevention and treatment of liver fibrosis. PMID: 31975615 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - January 25, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells.
Authors: Zhang J, Si J, Gan L, Guo M, Yan J, Chen Y, Wang F, Xie Y, Wang Y, Zhang H Abstract Cervical cancer is the second most common malignant tumour threatening women's health. In recent years, heavy-ion beam therapy is becoming a newly emerging therapeutic mean of cancer; however, radio-resistance and radiation-induced damage constitute the main obstacles for curative treatment of cervical cancer. Therefore, to identify the radiosensitizers is essential. Here, we investigated the effects of Wnt signalling pathway on the response of 12C6+ radiation in HeLa cells. XAV939, an inhibitor of Wnt signalling pathway, w...
Source: Artificial Cells, Nanomedicine and Biotechnology - January 25, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Physcion 8-O- β-glucopyranoside exerts protective roles in high glucose-induced diabetic retinopathy via regulating lncRNA NORAD/miR-125/STAT3 signalling.
Physcion 8-O-β-glucopyranoside exerts protective roles in high glucose-induced diabetic retinopathy via regulating lncRNA NORAD/miR-125/STAT3 signalling. Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):463-472 Authors: Wan W, Wan W, Long Y, Li Q, Jin X, Wan G, Zhang F, Lv Y Abstract Diabetic retinopathy (DR) is the leading cause of decreased vision and blindness globally. The aim of this study was to understand the role of physcion 8-O-β-glucopyranoside (PG) in high glucose (HG)-induced DR and to investigate whether lncRNA NORAD/miR-125/STAT3 signalling was the underlying mechanism involved in...
Source: Artificial Cells, Nanomedicine and Biotechnology - January 14, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

MiR-142-3p targeting NUCKS1 inhibits proliferation and invasion of pancreatic cancer cells.
Conclusion: MiR-142-3p regulated the biological characteristics of pancreatic cancer cells by directly targeting NUCKS1. PMID: 31914803 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - January 11, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Regenerated silk fibroin (RSF) electrostatic spun fibre composite with polypropylene mesh for reconstruction of abdominal wall defects in a rat model.
Authors: Yang D, Song Z, Shen J, Song H, Yang J, Zhang P, Gu Y Abstract Abdominal wall defects are associated with abdominal wall surgery, infection and tumour resection. Polypropylene (PP) mesh, which has excellent mechanical strength, is currently the primary clinical repair material. In repairing the abdominal wall, the mesh can erode the bowel and cause other problems. Constructing a barrier that induces a weak inflammatory response and promotes rapid recovery of the peritoneum is important. We used electrospinning technology to construct a silk fibroin coating on the abdominal surface of a PP patch. A rat mode...
Source: Artificial Cells, Nanomedicine and Biotechnology - January 11, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Rs145204276 and rs4759314 affect the prognosis of prostate cancer by modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathways.
This study aims to characterize the role of GAS5 rs145204276 and HOTAIR rs4759314 polymorphisms in the pathogenesis of PC. Both INS allele of GAS5 rs145204276 and A allele of HOTAIR rs4759314 were identified to increase the survival of PC patients. And patients carrying DEL/DEL + AG genotypes tend to present higher levels of HMGB1, GAS5, HOTAIR and lower levels of miR-1284 and miR-22. In addition, the transcription activity of GAS5 promoter was increased by the deletion allele of rs145204276 polymorphism, while the G allele of rs4759314 polymorphism increased the transcription activity of HOTAIR promoter. GAS...
Source: Artificial Cells, Nanomedicine and Biotechnology - January 11, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Clostridium difficile toxin B induces colonic inflammation through the TRIM46/DUSP1/MAPKs and NF- κB signalling pathway.
Clostridium difficile toxin B induces colonic inflammation through the TRIM46/DUSP1/MAPKs and NF-κB signalling pathway. Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):452-462 Authors: Li Y, Xu S, Xu Q, Chen Y Abstract Clostridium difficile (C. difficile) infection results in toxin-induced epithelial injury and marked colonic inflammation. Mitogen-activated protein kinase (MAPK) and NF-κB which regulated by MAP kinase phosphatase (MKP, also known as dual specificity phosphatases, DUSP) are fundamental signalling pathways that mediate multiple cellular processes. However, the regulation of DU...
Source: Artificial Cells, Nanomedicine and Biotechnology - January 11, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

LncRNA PTENP1 inhibits cervical cancer progression by suppressing miR-106b.
This study investigates the interaction of PTENP1 and miR-106b in cell proliferation, apoptosis and epithelial-mesenchymal transition (EMT) in cervical cancer. The expressions of PTENP1, miR-106b and PTEN were determined in cervical cancer tissues, adjacent normal tissues, cervical cancer cells (HeLa, SiHa, C33A and CasKi) and normal cervical epithelial H8 cells. Up-regulation of PTENP1 and down-regulation of miR-106b were conducted in HeLa and CasKi cells by transfecting cells with corresponding miRNA mimics and inhibitors. Bioinformatics analysis, luciferase reporter assay and RNA-pull down assay were performed to verify...
Source: Artificial Cells, Nanomedicine and Biotechnology - January 10, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Preliminary biological evaluation of 123I-labelled anti-CD30-LDM in CD30-positive lymphomas murine models.
Authors: Gong J, Guo F, Cheng W, Fan H, Miao Q, Yang J Abstract Overexpression of CD30 has been reported on the surface of some T-cell lymphomas, especially on Hodgkin's lymphoma (HL) and anaplastic large cell lymphoma (ALCL). CD30 targeted immunotherapy has good clinical therapy response. We have produced a novel antibody drug conjugates (ADCs)-anti-CD30-LDM, which shows attractive tumour-targeting capability and extremely potent antitumor efficacy. To further investigate biological characteristics and promote clinical translation of anti-CD30-LDM, we constructed a radiolabeled 123I-anti-CD30-LDM to evaluate the b...
Source: Artificial Cells, Nanomedicine and Biotechnology - January 10, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Protective effect of hyperoside against hydrogen peroxide-induced dysfunction and oxidative stress in osteoblastic MC3T3-E1 cells.
This study aims to evaluate the protective effects provided by HYP to osteoblastic MC3T3-E1 cells. MC3T3-E1 cells were pre-treated with HYP for 24 h before being treated with 0.3 mM hydrogen peroxide (H2O2) for 24 h. Cell viability, flow cytometric analysis and mRNA expression of alkaline phosphatase (ALP), collagen I (COL-I) and osteocalcin (OCN) in MC3T3-E1 cells were examined. We next examined apoptosis-related and mitogen-activated protein kinase (MAPK) related proteins in HYP and H2O2 groups. HYP over the dose of 40 μmol/L could obviously increase the MC3T3-E1 cell viability at 24&thinsp...
Source: Artificial Cells, Nanomedicine and Biotechnology - January 8, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Anti-antioxidant impacts of circZNF609 silence in HaCaT cells through regulating miR-145.
Conclusion: The protective function of cZNF609 silence in H2O2-injured HaCaT cells was revealed in vitro. Silence of cZNF609 exhibited its impact possibly through regulating miR-145, and JNK and p38MAPK pathways. PMID: 31905030 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - January 8, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Prognostic and clinicopathologic significance of long non-coding RNA opa-interacting protein 5-antisense RNA 1 in multiple human cancers.
Conclusion: High expression of lncRNA OIP5-AS1 may predict a poor OS and advanced clinical stage, implicating that OIP5-AS1 may be a possible prognostic factor in cancers. PMID: 31899963 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - January 5, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Prokineticin 2 relieves hypoxia/reoxygenation-induced injury through activation of Akt/mTOR pathway in H9c2 cardiomyocytes.
In conclusion, PK2 relieved H/R-induced injury in H9c2 cardiomyocytes by activation of the Akt/mTOR pathway. PMID: 31899964 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - January 5, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Histological and functional outcomes in a rat model of hemisected spinal cord with sustained VEGF/NT-3 release from tissue-engineered grafts.
This study verifies that co-delivery of VEGF/NT-3 reduces inflammation and gliosis in the hemisected spinal cord, promotes axonal outgrowth and results in better locomotor recovery, while the BMSCs transplantation facilitates these functions limitedly. PMID: 31899965 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - January 5, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Cytoplasmic p27 is a novel prognostic biomarker and oncogenic protein for nasopharyngeal carcinoma.
Authors: Teng Y, Hu L, Yu B, Li X, Chen M, Fu X, Zhang J, Gao Y, Xu R, Zhu J Abstract Cytoplasmic p27 plays an important role in regulating the cell cycle. Recent studies have revealed p27 protein translocation from the nucleus to the cytoplasm in many tumour cells. The aim of this study was to investigate the role and molecular mechanisms of cytoplasmic p27 in the progression of nasopharyngeal carcinoma (NPC) and to explore its prognostic value. We found increased cytoplasmic p27 expression by immunohistochemistry in NPC tissues, and its expression level was significantly correlated with the T classification and T...
Source: Artificial Cells, Nanomedicine and Biotechnology - January 1, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Long non-coding RNA HULC exerts oncogenic activity on papillary thyroid cancer in  vitro and in vivo.
Long non-coding RNA HULC exerts oncogenic activity on papillary thyroid cancer in vitro and in vivo. Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):326-335 Authors: Yang Z, Li G, Ding C, Sun W, Zhang J Abstract Thyroid cancer is a frequently happened malignancy in human endocrine system. Papillary thyroid cancer (PTC) presents 70-80% of all thyroid cancer cases. Herein, we probed the possible oncogenic function of long non-coding RNA (lncRNA) highly up-regulated in liver cancer (HULC) in PTC. First, the HULC and microRNA-106a (miR-106a) expressions in PTC tissues and cells were tested. Plasmi...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 29, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Silencing hsa_circ_PVT1 (circPVT1) suppresses the growth and metastasis of glioblastoma multiforme cells by up-regulation of miR-199a-5p.
Conclusion: Our data suggested that silencing circPVT1 inhibited viability, migration, EGF-induced EMT and promoted apoptosis as well as repressed YAP1 and PI3K/AKT pathways by up-regulating miR-199a-5p.HIGHLIGHTSCircPVT1 expression is highly expressed in GBM tissues;Si-circPVT1 represses migration and promoted apoptosis in U539 and U251 cells;Si-circPVT1 represses migration and promoted apoptosis when elevating miR-199a-5p;Si-circPVT1 represses EGF-induced EMT when increasing miR-199a-5p;Si-circPVT1 suppresses YAP1 and PI3K/AKT pathways by up-regulating miR-199-5p. PMID: 31865777 [PubMed - in process] (Source: Artific...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 25, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Correction.
Authors: PMID: 31873037 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - December 25, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

miR-324-5p inhibits gallbladder carcinoma cell metastatic behaviours by downregulation of transforming growth factor beta 2 expression.
Authors: Zhang X, Zhang L, Chen M, Liu D Abstract Increasing studies have demonstrated that microRNAs (miRNAs) are associated with the metastasis of gallbladder carcinoma (GBC). Recently, miR-324-5p has been reported to be a tumour-suppressive miRNA in many types of malignant cancer. However, the biological function and molecular mechanism of miR-324-5p in GBC still remain largely unknown. Here, we found that miR-324-5p expression was notably down-regulated in both GBC tissues and cells compared with that in normal controls. Downregulated miR-324-5p expression was negatively associated with the status of local inva...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 22, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Construction of an immune-related genes nomogram for the preoperative prediction of axillary lymph node metastasis in triple-negative breast cancer.
Authors: Tan W, Xie X, Huang Z, Chen L, Tang W, Zhu R, Ye X, Zhang X, Pan L, Gao J, Tang H, Zheng W Abstract Immune system disorder is associated with metastasis of triple-negative breast cancers (TNBCs). A robust, individualized immune-related genes (IRGs)-based classifier was aimed to develop and validate in our study to precisely estimate the axillary lymph node (ALN) status preoperatively in patients with early-stage TNBC. We first analyzed RNA sequencing profiles in TNBC patients from The Cancer Genome Atlas database by using bioinformatics approaches, and screened 23 differentially expressed IRGs. A 9-gene pa...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 22, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

MicroRNA-15a, microRNA-15b and microRNA-16 inhibit the human dopamine D1 receptor expression in four cell lines by targeting 3'UTR -12  bp to + 154 bp.
Conclusion: The miRNA-15a, miRNA-15b and miRNA-16 inhibit the human dopamine D1 receptor expression by targeting 3'UTR -12 to +154 bp.HighlightsDRD1 3'UTR ranging from -12 to +1135 bp was essential for the post-transcriptional regulation of miRNAs.The deletion of -12 to +154 bp fragment significantly increased the luciferase expression but not the mRNA expression.The miRNA-15a, miRNA-15b and miRNA 16 affected DRD1 expression in different cell lines, respectively. PMID: 31858826 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - December 22, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Improving radio-chemotherapy efficacy of prostate cancer by co-deliverying docetaxel and dbait with biodegradable nanoparticles.
In this study, we engineered the polycation nanoparticle (NP), which co-encapsulated DNA damage repair inhibitor Dbait and chemotherapeutic drug Docetaxel (Dtxl), using H1 nanopolymer (folate--polyethylenimine600-cyclodextrin), and the size of H1/Dbait/Dtxl was about 117 nm. We demonstrated that H1/Dbait/Dtxl enhanced the efficiency of radio-chemotherapy in prostate cancer cells by CCK-8 assay and colony-forming assay. Importantly, the improvement of radio-chemotherapy of H1/Dbait/Dtxl in prostate cancer was also validated in vivo, and the NP did not have a high toxicity profile. The results of immunohistochemi...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 22, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The protective effects of bexarotene against advanced glycation end-product (AGE)-induced degradation of articular extracellular matrix (ECM).
Authors: Zha Z, Han Q, Huo S Abstract Osteoarthritis (OA) is a common debilitating disease primarily characterised by excessive loss of the articular ECM, which is composed of up to 95% type II collagen. Among the factors that contribute to the pathogenesis of OA, the natural process of aging is regarded as the most significant risk factor. AGEs, which are extremely resilient to degradation, are produced in the body naturally as a result of the Maillard process of nonenzymatic glycation and are also introduced through diet and tobacco smoke. AGEs have a high affinity for collagen and therefore accumulate in joint t...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 21, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

PHD2 acts as an oncogene through activation of Ras/Raf/MEK/ERK and JAK1/STAT3 pathways in human hepatocellular carcinoma cells.
Conclusion: PHD2 exhibited pro-tumour functions in HCC cells. PHD2 promoted HCC possibly through Ras/Raf/MEK/ERK and JAK1/STAT3 pathways.HighlightsPHD2 is highly expressed in HCC tissue and cell lines;PHD2 promotes the proliferation of Huh7 and HepG3B cells;PHD2 enhances Huh7 and HepG3B cells migration and invasion;PHD2 activates Ras/Raf/MEK/ERK and JAK1/STAT3 signalling. PMID: 31852247 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - December 21, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The protective role of omentin-1 in IL-1 β-induced chondrocyte senescence.
In this study, we examined the biological function of omentin-1 in cultured chondrocytes. The presence of omentin-1 potently suppresses IL-1β-induced cellular senescence as revealed by staining with senescence-associated beta-galactosidase (SA-β-Gal). At the cellular level, omentin-1 attenuates IL-1β-induced G1 phase cell-cycle arrest. Mechanistically, we demonstrate that omentin-1 reduced IL-1β-induced expression of senescent factors including caveolin-1, p21, and PAI-1 as well as p53 acetylation through ameliorating SIRT1 reduction. Notably, silencing of SIRT1 abolishes IL-1β-induced senescence a...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 21, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Quantitative assessment of lncRNA H19 polymorphisms and cancer risk: a meta-analysis based on 48,166 subjects.
Authors: Liu X, Zhao Y, Li Y, Zhang J Abstract Recently, numerous studies have been performed to detect the association between H19 polymorphisms and cancer susceptibility. However, their results were inconsistent and controversial. So, we carried out a meta-analysis aiming to define the association exactly. Eligible studies were collected using PubMed and Embase databases up to March 31, 2019. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to assess the strength of association. Sensitivity analysis and publication bias were established to evaluate the robustness of our results. Totally, 60 s...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 21, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Cytisine exerts anti-tumour effects on lung cancer cells by modulating reactive oxygen species-mediated signalling pathways.
In conclusion, cytisine plays a critical role in suppressing the carcinogenesis of lung cancer cells through cell cycle arrest and induction of mitochondria-mediated apoptosis, suggesting that it may be a promising candidate for the treatment of human lung cancer. PMID: 31852250 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - December 21, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

MicroRNA-210 protects against periodontitis through targeting HIF-3 α and inhibiting p38MAPK/NF-κB pathway.
MicroRNA-210 protects against periodontitis through targeting HIF-3α and inhibiting p38MAPK/NF-κB pathway. Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):129-136 Authors: Jia S, Yang X, Yang X, Zhang F Abstract The aim of this study was to investigate the effects of miR-210 abnormal expression on Porphyromonas gingivalis lipopolysaccharide (LPS)-treated primary human periodontal ligament cells (PDLCs). The miR-210 level was identified in gingival tissues from patients with chronic periodontitis (CP) and healthy subjects as well as LPS-treated PDLCs by qRT-PCR. Cell viability, apoptotic cell...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 21, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Gellable silk fibroin-polyethylene sponge for hemostasis.
In conclusion, SF is a new type of haemostatic material that might be able to meet the requirements of speed, efficiency and biosafety in a variety of clinical applications. PMID: 31852256 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - December 21, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research