Geniposide alleviates lipopolysaccharide-caused apoptosis of murine kidney podocytes by activating Ras/Raf/MEK/ERK-mediated cell autophagy.
This study explored the effects of geniposide (GEN) on lipopolysaccharide (LPS)-caused murine kidney podocyte MPC5 apoptosis and autophagy. Viability and apoptosis of MPC5 cells were respectively detected with the help of CCK-8 assay and Guava Nexin assay. 3-Methyladenine (3-MA) was used as an autophagy inhibitor, while rapamycin as autophagy activator. Si-Beclin-1 was transfected in MPC5 cells to down-regulate the expression of Beclin-1. We found that LPS stimulation significantly caused MPC5 cell viability reduction, apoptosis and autophagy (P
Source: Artificial Cells, Nanomedicine and Biotechnology - April 17, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

A novel cross-linked haemoglobin-based oxygen carrier is beneficial to sepsis in rats.
Authors: Kuang L, Zhu Y, Zhang J, Wu Y, Tian K, Chen X, Xue M, Tzang FC, Lau B, Wong BL, Liu L, Li T Abstract Pathological hypoxia-induced organ dysfunction contributes to the high mortality of sepsis. Because of the microcirculation dysfunction following severe sepsis, it is difficult for erythrocytes to transport oxygen to hypoxic tissues. Haemoglobin-based oxygen carriers (HBOCs) are capable of delivering oxygen to hypoxic tissues. The aim of this study is to observe the potential benefits of a novel bovine-derived, non-polymerized, cell-free HBOC solution, YQ23, on sepsis in rats. Cecum ligation and puncture wa...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 17, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Relationship between the expressions of PD-L1 and tumour-associated fibroblasts in gastric cancer.
In conclusion, TAFs promoted the growth in gastric cancer cell lines by increased the PD-L1 expression. PMID: 30983436 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 17, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Long non-coding RNA SNHG16 reduces hydrogen peroxide-induced cell injury in PC-12 cells by up-regulating microRNA-423-5p.
In conclusion, these findings indicated that SNGH16 reduced H2O2-evoked cell injury by mediating miR-423-5p in PC-12 cells. The findings might uncover the effect of SNHG16 on SCI, which provide a new reference for remedying SCI. Highlights H2O2 evokes cell injury in PC-12 cells; SNHG16 reduces H2O2-induced cell injury in PC-12 cells; SNGH16 enhances miR-423-5p expression in H2O2-stimulated PC-12 cells; MiR-423-5p inhibition abrogates the protective effect of SNGH16 in PC-12 cells; SNGH16 mediates AMPK and ERK1/2 pathways by up-regulating miR-423-5p. PMID: 30977409 [PubMed - in process] (Source: Artificial Cells, Nanome...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 14, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Co-delivery of sorafenib and VEGF-siRNA via pH-sensitive liposomes for the synergistic treatment of hepatocellular carcinoma.
Authors: Yao Y, Wang T, Liu Y, Zhang N Abstract Non-viral nanocarrier affords a platform for drug and siRNA combination, the focus of which is to load drug and siRNA into a single carrier, allowing for co-delivery and a synergistic effect at tumour site. In our previous study, pH-sensitive carboxymethyl chitosan-modified liposomes (CMCS-SiSf-CL) were assembled for sorafenib (Sf) and Cy3-siRNA co-loaded. The present study evaluated in vitro and in vivo co-delivery of the co-loaded liposomes. Further, in vitro inhibiting hepatocellular carcinoma of the pH-sensitive sorafenib (Sf) and VEGF-siRNA co-loaded liposomes wa...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 14, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The therapeutic effect of secretome from human umbilical cord-derived mesenchymal stem cells in age-related osteoporosis.
In conclusion, these findings demonstrated that secretome from hUCMSCs has the capacity to recover stem cell potential and delay local bone loss in age-related osteoporosis, which could potentially be applied in osteoporosis therapy in the future. PMID: 30977425 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 14, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Explorative analysis of the gene expression profile during liver regeneration of mouse: a microarray-based study.
In conclusion, these findings could contribute to revealing the molecular mechanism of liver regeneration after PH, which could provide new ideas and treatment methods for regenerative medicine, oncological drug development and oncological treatment. PMID: 30963776 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 11, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Antagonism of RIP1 using necrostatin-1 (Nec-1) ameliorated damage and inflammation of HBV X protein (HBx) in human normal hepatocytes.
Authors: Xie L, Huang Y Abstract Hepatitis B virus X protein (HBx), encoded by the hepatitis B virus (HBV) genome, plays a pivotal in mediating pathogenicity in liver diseases. However, the underlying mechanisms are still needed to be elucidated. Receptor-interacting protein 1 (RIP1) has been identified as a serine/threonine kinase with various biological functions in cell fate, proliferation, and death. In the current study, we found that overexpression of HBx in LO2 human normal hepatocytes increased the expression of RIP1. Importantly, our results indicate that blockage of RIP1 using its specific antagonist necr...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 11, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Inhibition of SF3b1 by pladienolide B evokes cycle arrest, apoptosis induction and p73 splicing in human cervical carcinoma cells.
Authors: Zhang Q, Di C, Yan J, Wang F, Qu T, Wang Y, Chen Y, Zhang X, Liu Y, Yang H, Zhang H Abstract Pladienolide B is a potent cancer cell growth inhibitor that targets the SF3b1 subunit of the spliceosome. There is considerable interest in the compound as a tool to study SF3b1 function in cancer. However, so far little information is available on the molecular mechanism of SF3b1 eliciting apoptosis in cancer cells. Here, we investigated the molecular mechanism of SF3b1 eliciting apoptosis in human cervical carcinoma cells. We demonstrated that inhibition of SF3b1 by pladienolide B inhibited proliferation of HeLa...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 11, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

IL-33 drives the antitumour effects of dendritic cells via upregulating CYLD expression in pulmonary adenocarcinoma.
In conclusion, our study demonstrated that IL-33 mediated immunoregulation in pulmonary adenocarcinoma by improving DC-induced T cell proliferation by upregulating CYLD expression. PMID: 30964341 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 11, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Using CuO nanoparticles and hyperthermia in radiotherapy of MCF-7 cell line: synergistic effect in cancer therapy.
Authors: Esmaeili Govarchin Ghaleh H, Zarei L, Mansori Motlagh B, Jabbari N Abstract The aim of this paper was examining the combined impacts of CuO nanoparticles (CuO NPs), hyperthermia (H), and irradiation (R) on an increment of MCF-7 cells. The MTT assay was employed to assess the antiproliferative effects of CuO NPs (25, 50, and 100 μg/ml), hyperthermia (41 °C for 1 h), and irradiation (200 cGy). Moreover, the perniciousness was estimated through the survival capability of cells, and apoptosis, ROS production, and levels of caspase-3, -8 and -9 proteins were determined. A significant (p
Source: Artificial Cells, Nanomedicine and Biotechnology - April 11, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Photosensitizer effects of MWCNTs-COOH particles on CT26 fibroblastic cells exposed to laser irradiation.
Authors: Abdi Goushbolagh N, Keshavarz M, Zare MH, Bahreyni-Toosi MH, Kargar M, Farhood B Abstract In the current study, photosensitizer effect of carboxylate multiwalled carbon nanotubes (MWCNTs-COOH) on CT26 fibroblastic cells following near infrared (NIR) irradiation was quantized in photo-thermal therapy (PTT). Moreover, it was tried to achieve optimal dose of MWCNTs-COOH and laser exposure time. Characterizations of MWCNTs-COOH were scrutinized using scanner electron microscope (SEM), spectrophotometer, and particle size analyzer. The seeded CT26 cells were treated with nontoxic concentrations of MWCNTs-COOH a...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 11, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

LncRNA HIF2PUT inhibited osteosarcoma stem cells proliferation, migration and invasion by regulating HIF2 expression.
CONCLUSIONS: Overexpression of lncRNA HIF2PUT significantly attenuated the proliferation, migration and invasion of osteosarcoma stem cells. Furthermore, we demonstrated that lncRNA overexpression inhibited the sphere-formation of osteosarcoma stem cells by downregulating HIF-2α. These findings suggest that lncRNA HIF2PUT may act as a tumour suppressor in osteosarcoma. LncRNA HIF2PUT/HIF-2α may be a novel therapeutic target in the treatment of osteosarcoma. PMID: 30966832 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 11, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

LDH hybrid thermosensitive hydrogel for intravaginal delivery of anti-HIV drugs.
In this study, a hybrid thermo-sensitive hydrogel constituted by nanosized layered double hydroxides and poloxamer 407 (P407) was constructed and co-loaded with both hydrophobic and hydrophilic drug. The LDH-P407 hydrogel could achieve sol-gel transition at body temperature. The in vivo experiment showed that LDH-P407 hydrogel can achieve controlled release of theaflavin and Nile red (hydrophobic drug model) into blood by vaginal drug delivery, meanwhile the hydrogel showed barely mucosal irritation. In addition, ex vivo experiment showed that the nifeviroc-loaded LDH-P407 hydrogel was able to specifically bind co-receptor...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 11, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

CircCDYL inhibits the expression of C-MYC to suppress cell growth and migration in bladder cancer.
In this study, the expression level of circCDYL was detected by quantitative real-time PCR in bladder cancer tissues. The circCDYL over-expression plasmid was constructed and transfected into bladder cancer cell lines. The cell migration was detected by using transwell migration assay and wound healing assay, and cell cycles were detected by flow cytometry. The relative protein expression was detected by Western blotting. It was found that circCDYL was low expressed in bladder cancer tissues and cell lines. Functionally, over-expression of circCDYL inhibited cell growth and migration. Importantly, over-expression of circCD...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 11, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The mechanism of miR-16-5p protection on LPS-induced A549 cell injury by targeting CXCR3.
Authors: Liu GP, Wang WW, Lu WY, Shang AQ Abstract OBJECTIVE: To study the effect of miR-16-5p on lung cancer cell injury and apoptosis, and its mechanism. METHODS: LPS induced lung cancer cell A549 injury; qRT-PCR method was applied to detect the expression of miR-16-5p and CXCR3 in A549 cells. Con (without LPS treatment), LPS + miR-NC group (transfected negative control samples), LPS + miR-16-5p group (transfected miR-16-5p mimics); LPS + si-NC group (transfected negative control samples), LPS + si-CXCR3 group (transfected si-CXCR3); LPS + miR-16-5p + pcDNA3.1 group (co-transfected miR-16-5p mimics and pcDNA3...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 10, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Correction.
Authors: PMID: 30939932 [PubMed] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Expanded 3D nanofibre sponge scaffolds by gas-foaming technique enhance peripheral nerve regeneration.
In conclusion, we design a new method to construct a 3D scaffold containing NGC with orientation and porosity. The application of this 3D scaffold material has good prospects in future peripheral nerve repair. PMID: 30942090 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Salicin inhibits AGE-induced degradation of type II collagen and aggrecan in human SW1353 chondrocytes: therapeutic potential in osteoarthritis.
Authors: Gao F, Zhang S Abstract Osteoarthritis (OA) is a major age-related disease, which may be caused by the accumulation of advanced glycation end-products (AGEs). Excessive degradation of type II collagen and aggrecan by matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase with thrombospondin type 1 motif (ADAMTS) induced by AGEs is a pivotal event in the pathogenesis of osteoarthritis. In addition, activation of the nuclear factor-κB (NF-κB) pathway induces the expression of a cascade of proinflammatory cytokines, such as interleukin (IL)-1β and tumor necrosis factor-&alph...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Associations between IL-23R gene polymorphisms and the susceptibility of rheumatoid arthritis: a meta-analysis.
CONCLUSION: The results of meta-analysis indicated that IL-23R gene rs2201841 polymorphism might be a susceptible factor for RA under G vs. A model, especially in Caucasian population. PMID: 30942097 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Analysis of polymorphisms in genes associated with the FA/BRCA pathway in three patients with multiple primary malignant neoplasms.
This study analyzed the genetic susceptibility of gene polymorphisms in three patients with multiple primary malignant neoplasms and examined the possible pathogenesis. The clinical data and whole genome sequence of three patients (1 with 5 primary cancers, 1 with 4 primary cancers, and 1 with 3 primary cancers) were aligned with a series of databases. We found the three patients contained a total of seven types of malignant tumours (endometrial cancer, ovarian cancer, breast cancer, colon cancer, ureter cancer, bladder cancer and kidney cancer). It was found that the varied genes in Patient 1 (5 primary cancers) were BRIP...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Design, synthesis and characterization of enzyme-analogue-built polymer catalysts as artificial hydrolases.
Authors: Mathew D, Thomas B, Devaky KS Abstract In this review, the concept and various strategies in molecular imprinting is discussed briefly. How the concept of transition state analogue can be used to design a template to prepare catalytic imprinted polymers is described in detail. The use of the "bait and switch" approach and alternative covalent template strategies show how functional groups which assist in the catalytic properties can be assembled within the imprint. Thus, there are so many reports on P catalyzed reactions. Owing to their advantageous properties over natural biological recognition ...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Synthesis and characterization of gold nanoparticles from aqueous leaf extract of Alternanthera sessilis and its anticancer activity on cervical cancer cells (HeLa).
Authors: Qian L, Su W, Wang Y, Dang M, Zhang W, Wang C Abstract Cervical cancer is the third most common highest mortality in women worldwide. The use of standard chemotherapeutic drugs against cervical cancer patients received several side effects. Therefore, we focused phytoconsituents-mediated synthesis of gold nanoparticles (AuNPs) considered as greatest attention in the treatment of cervical cancer. In this present study, we reported that green synthesis of AuNPs by using with Alternanthera Sessilis aqueous extract. Synthesis of AuNPs were characterized by UV visible spectroscopy, energy dispersive X-ray (EDX)...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

A conceptual review of rhodanine: current applications of antiviral drugs, anticancer and antimicrobial activities.
Authors: Mousavi SM, Zarei M, Hashemi SA, Babapoor A, Amani AM Abstract Rhodanines are accepted as advantaged heterocycles in medicinal chemistry as one of the 4-thiazolidinones subtypes. The aim of this paper is to analyze the features of rhodanine and its application in pharmacy and medicine. Some of the properties of rhodanine such as antiviral, anticancer, antimicrobial, and drug discovery have recently been reported. Although there are still vague points in the structure and mechanism of polymerization of this substance, there is a significant increase in the use of rhodanine in medicine. In this review paper,...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Engineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cells.
Authors: Moosazadeh Moghaddam M, Bonakdar S, Shokrgozar MA, Zaminy A, Vali H, Faghihi S Abstract Differentiation of stem cells to Schwann is considered efficient way for nerve regeneration since the sources of human Schwann cells are limited for clinical application. It is demonstrated that mimicking micromechanical forces or micro/nanotopographical environments that stem cells are experienced in vivo could control their fate. Here, the potency of substrates with imprinted cell-like topographies for direct differentiation of adipose-derived mesenchymal stem cells (ADSCs) into Schwann cells (SCs) is reported. For th...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Safeguarding the catalytic activity and stability of polyaniline chitosan silver nanocomposite bound beta-galactosidase against product inhibitors and structurally related compound.
In this study, an attempt has been made to evaluate the effect of products of β-galactosidase (βGS) catalyzed reaction i.e. glucose and galactose and their structurally related compound vitamin C (VC) on the catalytic activity of native and PANI-CS-NC and PANI-CS-Ag-NC adsorbed βGS. Results indicated a decline in catalytic activity of soluble enzyme in the presence of all investigated compounds. The order of inhibition was found to be VC 
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Lixisenatide attenuates advanced glycation end products (AGEs)-induced degradation of extracellular matrix in human primary chondrocytes.
Authors: Li X, Jia F, Zhu Z, Huang L Abstract Osteoarthritis (OA) poses a growing threat to the health of the global population. Accumulation of advanced glycation end-products (AGEs) has been shown to upregulate expression of degradative enzymes such as matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) in chondrocytes, which leads to excessive degradation of type II collagen and aggrecan in the articular extracellular matrix (ECM). In the present study we investigated the effects of the GLP-1 agonist lixisenatide, a widely used type II diabetes medication,...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Ras-ERK1/2 signalling promotes the development of osteosarcoma through regulation of H4K12ac through HAT1.
In conclusion, these results testified that Ras-ERK1/2 signalling promoted the development of OS by mediating H4K12ac through MDM2-mediated HAT1 degradation. PMID: 30942624 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Minicapsules encapsulating nanoparticles for targeting, apoptosis induction and treatment of colon cancer.
Authors: Jain A, Jain R, Jain S, Khatik R, Veer Kohli D Abstract Cancer therapies are aimed at eliminating the rapidly growing tumor cells by surgery and radiotherapy. The present therapies are only fruitful in early identified cases. The present study involves the preparation and characterization of eudragit S100-coated mini-capsules filled with chitosan nanoparticles-unconjugated and folic acid (FA)-conjugated encapsulating caspase 3 activator (7-hydroxystaurosporine). The formulated nanoparticles were compared for the cancer targeting and curing ability of the same by pre-investigation through drug release in or...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Improved antibacterial function of Rifampicin-loaded solid lipid nanoparticles on Brucella abortus.
The objective of this study was to assess the antibacterial activity of Rifampicin-loaded solid lipid nanoparticles on Brucella abortus 544. Rifampicin-loaded solid lipid nanoparticles were prepared by a modified microemulsion/sonication method and characterized. The results showed the average size about 319.7 nm, PI about 0.20 and zeta potential about 18.4 mv, encapsulation efficacy and drug-loading were equal to 95.78 and 34.2%, respectively, with a spherical shape. Drug release lasted for 5 days. The antibacterial activity was statistically significant with p 
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Synthesis of gold nanoparticles from leaf Panax notoginseng and its anticancer activity in pancreatic cancer PANC-1 cell lines.
Authors: Wang L, Xu J, Yan Y, Liu H, Li F Abstract Development of novel methods is needed for the synthesis of nanoparticles. Attention on such particles has elevated disquiet about the eco-friendly manner of their fabrication methods. In the present study, we equipped and synthesized gold nanoparticles (AuNPs) from Panax notoginseng; investigated as an environmentally friendly and non-toxic substrate. Amalgamation of AuNPs was distinguished by numerous studies such as UV-absorbance and it shows peak values in the range of 520-550 nm. Nanoparticles sizes are confirmed by dynamic light scattering analysis and it sho...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Biosynthesis of anti-leishmanial natural products in callus cultures of Artemisia scoparia.
In this study, various extracts of the callus cultures of Artimisia scoparia established in response to different plant growth regulators (PGRs) were evaluated for their anti-leishmanial effects against Leishmania tropica promastigotes, followed by an investigation of the possible mechanism of action through reactive apoptosis assay using fluorescent microscopy. Amongst the different callus extracts, higher anti-leishmanial activity (IC50:19.13 µg/mL) was observed in the callus raised in-vitro in the presence of 6-Benzylaminopurine (BA) plus 2,4-Dichlorophenoxyacetic Acid (2,4-D) at the concentration of 1.5&th...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Propofol inhibits proliferation and epithelial-mesenchymal transition of MCF-7 cells by suppressing miR-21 expression.
In conclusion, propofol can inhibit the proliferation and EMT of MCF-7 cells by down-regulating miR-21 expression. Moreover, miR-21 can further regulate PI3K/AKT and Wnt/β-catenin pathways. PMID: 30942630 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Stem cells combined 3D electrospun nanofibrous and macrochannelled matrices: a preliminary approach in repair of rat cranial bones.
In this study, electrospun poly(ε-caprolactone) (PCL) membranes were spirally wounded to prepare 3D matrices composed of nanofibers and macrochannels. Mesenchymal stromal/stem cells were injected inside the scaffolds after the constructs were implanted in the cranial bone defects in rats. New bone formation, vascularisation and intramembranous ossification of the critical size calvarial defect were accelerated by using mesenchymal stem cells combined 3D spiral-wounded electrospun matrices. PMID: 30942631 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Kaempferol promotes proliferation, migration and differentiation of MC3T3-E1 cells via up-regulation of microRNA-101.
This study aimed to investigate the effects of kaempferol, a natural flavonoid compound, on osteoblast proliferation, migration and differentiation, as well as possible molecular mechanisms. qRT-PCR was performed to measure the expression level of microRNA-101 (miR-101). Cell viability and migration were respectively assessed using cell counting kit-8 (CCK-8) assay and two-chamber migration assay. Relative alkaline phosphatase (ALP) activity was evaluated using p-nitrophenyl phosphate (pNPP) as substrate. Western blotting was used to detect the protein expression levels of key molecules involved in cell proliferation, migr...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The effects of histamine H1 type receptor (H1R) in regulating osteoblastic cell differentiation and mineralization.
Authors: Sun Y, Peng X, Li Y, Ma H, Li D, Shi X Abstract Osteoblastic bone formation is important for maintaining the balance of bone turnover. However, the underlying mechanisms are still needed to be elucidated. Histamine H1 type receptor (H1R) is a major subtype of histamine membrane receptors family, which has displayed diverse biological functions in various tissues and cells. In the current study, we have identified a novel physiological function of H1R in regulating osteoblastic differentiation and mineralization of the MC3T3-E1 cells. We found that H1R is expressed in the MC3T3-E1 cells. Interestingly, H1R ...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The protective effects of saxagliptin against lipopolysaccharide (LPS)-induced inflammation and damage in human dental pulp cells.
In this study, we aimed to evaluate the protective effects of saxagliptin against LPS-induced intracellular insults in human dental pulp cells (HDPCs). We found that DPP-4 is expressed in HDPCs. Interestingly, the expression of DPP-4 was increased in response to LPS treatment. We also found that saxagliptin ameliorated LPS-induced production of ROS and reduction of glutathione (GSH). Additionally, saxagliptin prevented LPS-induced mitochondrial dysfunction by increasing the levels of mitochondrial membrane potential (MMP) and the production of adenosine triphosphate (ATP). Importantly, saxagliptin ameliorated LPS-induced r...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Circular RNA (hsa_circ_0051240) promotes cell proliferation, migration and invasion in ovarian cancer through miR-637/KLK4 axis.
In this study, we identified hsa_circ_0051240 was significantly increased in ovarian cancer (OC) tissues. Our results indicated that silencing of hsa_circ_0051240 inhibited OC cell proliferation, migration and invasion in vitro, and also prevented OC tumour formation in vivo. In addition, the inhibitory effects by blockage of hsa_circ_0051240 could be attenuated by the miR-637 inhibitor. Furthermore, we also identified that hsa_circ_0051240 act as a sponge of miR-637, and miR-637 directly targeted KLK4 mRNA in OC cells. Altogether, hsa_circ_0051240 promotes OC cell proliferation, migration and invasion through inhibiting t...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Production of Hypericum perforatum oil-loaded membranes for wound dressing material and in vitro tests.
Authors: Eğri Ö, Erdemir N Abstract Hypericum perforatum oil loaded electrospun polymeric wound dressing material was produced in order to be used in wound therapy. H. perforatum oil is known to have curative effect on wound-healing process. Wound dressing material was produced in two layers, upper layer of which was made of electrospun PCL nanofibres in order to maintain membrane integrity and mechanical strength, and bottom layer that is designed to be in contact with the wound was formed by electrospraying and electrospining of PEG/H. perforatum oil and PCL polymer solutions from opposite directions (concu...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Vildagliptin inhibits high free fatty acid (FFA)-induced NLRP3 inflammasome activation in endothelial cells.
In this study, we investigated the molecular mechanism of the clinically available DPP-4 inhibitor vildagliptin in the protection of FFA-induced endothelial dysfunction. Treatment of endothelial cells with vildagliptin inhibits FFA-induced cellular LDH release and generation of ROS. Vildagliptin also reverses FFA-induced reduced levels of GSH and elevated expression of the FFA-associated NAPHD oxidase protein NOX-4. Moreover, vildagliptin ameliorates the reduction in mitochondrial potential triggered by FFAs. Mechanistically, we show that vildagliptin suppresses FFA-induced expression of proteins of the NLRP3 inflammasome ...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

pPolyHb protects myocardial H9C2 cells against ischemia-reperfusion injury by regulating the Pink1-Parkin-mediated mitochondrial autophagy pathway.
In conclusion, pPolyHb has a protective effect on myocardial ischemia-reperfusion injury by regulating moderate mitochondrial autophagy. PMID: 30945565 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

LncRNA FOXD2-AS1 induces chondrocyte proliferation through sponging miR-27a-3p in osteoarthritis.
Authors: Wang Y, Cao L, Wang Q, Huang J, Xu S Abstract Osteoarthritis (OA) is a common degenerative joint disease worldwide. Long non-coding RNAs (lncRNAs) have been widely confirmed to involve in the modulation of OA progression. However, the underlying mechanisms of lncRNA FOXD2-AS1 in OA remain unclear. In the present study, we showed FOXD2-AS1 expression was upregulated and positively associated with the severity of OA patients. IL-1β and/or TNF-α treatment could increase FOXD2-AS1 expression in chondrocytes. FOXD2-AS1 overexpression induced cell proliferation, inflammation and extracellular matrix (...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

ARTIFICIAL CELL evolves into nanomedicine, biotherapeutics, blood substitutes, drug delivery, enzyme/gene therapy, cancer therapy, cell/stem cell therapy, nanoparticles, liposomes, bioencapsulation, replicating synthetic cells, cell encapsulation/scaffold, biosorbent/immunosorbent haemoperfusion/plasmapheresis, regenerative medicine, encapsulated microbe, nanobiotechnology, nanotechnology.
Authors: Chang TMS Abstract It is only in the last 20 years that many of the original ideas on artificial cells are being increasingly applied and extended by researchers around the world. Artificial cell has now evolved into nanomedicine, biotherapeutics, blood substitutes, drug delivery, enzyme/gene therapy, cancer therapy, cell/stem cell therapy, nanoparticles, liposomes, bioencapsulation, replicating synthetic cells, cell encapsulation/scaffold, biosorbent/immunosorbent haemoperfusion/plasmapheresis, regenerative medicine, encapsulated microbe, nanobiotechnology, nanotechnology and other areas. More futu...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 5, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Long non-coding RNA LINC00858 promotes cells proliferation, migration and invasion by acting as a ceRNA of miR-22-3p in colorectal cancer.
In this study, we firstly found that LINC00858 expression was significantly up-regulated in both CRC tissues and cell lines by both online data and RT-PCR assay. Then, clinical assay revealed that high LINC00858 expression was significantly associated with advanced clinical progression and poor prognosis. Multivariate analysis demonstrated that high LINC00858 expression was an independent poor prognostic factor for CRC patients. Moreover, lost-of-function assay indicated that knockdown of LINC00858 suppressed CRC cells proliferation, migration and invasion, and promoted apoptosis. Mechanistically, bioinformatics analysis, ...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 2, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheres.
In this study, microspheres prepared using poly(lactide-co-glycolide) (PLGA) and embedded with moxifloxacin (MOX-PLGA microspheres) and rifampicin/moxifloxacin (RIF/MOX-PLGA microspheres) using the water-in-oil-in-water double emulsion solvent evaporation technique were used for local delivery. Shape of MOX-PLGA microspheres and RIF/MOX-PLGA microspheres were spherical, mean particle size of them were 20.52 μm and 16.62 μm, respectively. Encapsulation efficiency of the MOX-PLGA microspheres was 17.35% ± 2.42%. However, the encapsulation efficiency for MOX and RIF in RIF/MOX-PLGA microspheres was ...
Source: Artificial Cells, Nanomedicine and Biotechnology - March 22, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Enhanced anti-proliferative and pro-apoptotic effects of metformin encapsulated PLGA-PEG nanoparticles on SKOV3 human ovarian carcinoma cells.
In conclusion, it is supposed that nano-encapsulation of MET into polymeric PLGA-PEG NPs may be a convenient drug delivery system to enhance its anticancer effects for ovarian cancer therapy. PMID: 30892093 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - March 22, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Long non-coding XIAP-AS1 regulates cell proliferation, invasion and cell cycle in colon cancer.
In this study, we found that XIAP-AS1 expression was significantly increased in CRC tissues and its expression showed a positive correlation with TNM stage and cumulative survival rate of CRC. To investigate whether XIAP-AS1 regulates the progression of CRC, we knocked down its expression in several CRC cell lines. CCK-8 assays showed that XIAP-AS1 knockdown remarkably suppressed CRC cell growth and arrested the cell cycle at the G0/G1 phase (flow cytometric analysis). Furthermore, XIAP-AS1 knockdown also remarkably blocked cell invasion of colon cancer cells by regulating the expression of EMT markers, such as E-cadherin,...
Source: Artificial Cells, Nanomedicine and Biotechnology - March 22, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Sustained release paclitaxel-loaded core-shell-structured solid lipid microparticles for intraperitoneal chemotherapy of ovarian cancer.
Authors: Han S, Dwivedi P, Mangrio FA, Dwivedi M, Khatik R, Cohn DE, Si T, Xu RX Abstract The current clinical paradigm for ovarian cancer treatment has a poor prognosis, partially due to the efficacy and toxicity concerns associated with the available chemotherapeutic formulations. To overcome these limitations, we have designed core-shell-structured paclitaxel (PTX) laden solid lipid microparticles (PTX-SLMPs) for intraperitoneal treatment of ovarian cancer. A single-step coaxial electro hydrodynamic atomization (CEHDA) process has been explored to synthesize core-shell structure of PTX-SLMPs with the particle si...
Source: Artificial Cells, Nanomedicine and Biotechnology - March 22, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The use of stromal vascular fraction (SVF), platelet-rich plasma (PRP) and stem cells in the treatment of osteoarthritis: an overview of clinical trials.
Authors: Mehranfar S, Abdi Rad I, Mostafav E, Akbarzadeh A Abstract Osteoarthritis (OA) is a major cause of disability across the world, which its prevalence is relatively high in elder population. Current accepted therapies such as exercise, anti-inflammatory drugs and intra-articular inoculation of corticosteroids are aimed at controlling symptoms in the affected patients. Surgical options including arthroplasty, osteotomy and joint replacement are other choices of treatment, which are invasive and can be applied in case of failure of conventional therapies. In the last few decades, efforts to treat musculoskelet...
Source: Artificial Cells, Nanomedicine and Biotechnology - March 21, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Development and evaluation of doxorubicin self nanoemulsifying drug delivery system with Nigella Sativa oil against human hepatocellular carcinoma.
DISCUSSION: The selected formulation (F6) has a mean particle size of 79.7 nm with PDI 0.098 and the minimum viscosity of 16.42 cps with % transmittance of 1.332 with maximum drug release of 96.968% in 32 h as compared to DOX alone. Stability data showed stable emulsion in both 250C and -40C. F6 showed improved efficacy in HepG2 cells by cytotoxicity, showed significant results p
Source: Artificial Cells, Nanomedicine and Biotechnology - March 21, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research