Radioactive gold nanocluster (198-AuNCs) showed inhibitory effects on cancer cells lines.
In this study, we produced, characterized and evaluated (in vitro) in three different cancer cell lines (protaste, breast and melanoma) a radioactive gold nanocluster (R-AuNC) (198Au25(Capt)18). The pharmacokinetics as the influence in the ABC transporter (MRP1 Efflux Transporter Protein) was also evaluated. The results showed that R-AuNC (198Au25(Capt)18) are capable to kill the cancer cells lines of protaste, breast and melanoma. The pharmacokinetics showed a fast clearance and great volume of distribution, confirming the use of R-AuNC as nanomedicine for cancer treatment. Finally, the ABC transporter assay corrobor...
Source: Artificial Cells, Nanomedicine and Biotechnology - September 18, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Development of graphene based nanocomposites towards medical and biological applications.
Authors: Mousavi SM, Low FW, Hashemi SA, Lai CW, Ghasemi Y, Soroshnia S, Savardashtaki A, Babapoor A, Pynadathu Rumjit N, Goh SM, Amin N, Tiong SK Abstract Graphene and its derivative materials present high potential towards medical and biological applications, including drug delivery and bioimaging, due to their exceptional properties such as thermal conductivity and high specific surface area. The main focus of this work is to review the current development of graphene materials and the derivatives for biocompatible, bioimaging and drug delivery applications. Also, the synthesis methods with variation of graphene...
Source: Artificial Cells, Nanomedicine and Biotechnology - September 17, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

5-Aminolevulinic acid hydrochloride loaded microbubbles-mediated sonodynamic therapy in pancreatic cancer cells.
Authors: Yang W, Xu H, Liu Q, Liu C, Hu J, Liu P, Fang T, Bai Y, Zhu J, Xie R Abstract 5-Aminolevulinic acid hydrochloride (ALA)-mediated sonodynamic therapy (SDT) had anti-tumour effect on pancreatic cancer cells. Hence, ALA loaded lipid/poly(lactic-co-glycolic acid) (PLGA) microbubbles (MBs)-mediated SDT for pancreatic cancer has great potential. The average size of ALA-lipid MBs and ALA-PLGA MBs was about 3.0 µm. The two kinds of MBs had good biocompatibility to normal HPDE6-C7 cells and were not toxic to pancreatic cancer cells. Compared with ALA-induced SDT, a statistically significant decrease in c...
Source: Artificial Cells, Nanomedicine and Biotechnology - September 15, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Green synthesis of copper nanoparticles using Cissus vitiginea and its antioxidant and antibacterial activity against urinary tract infection pathogens.
Authors: Wu S, Rajeshkumar S, Madasamy M, Mahendran V Abstract The green approachable of metal nanoparticles is treated to be an eco-friendly path and cost-effectiveness. In this present study, nano copper was synthesized profitably by Cissus vitiginea. The synthesized nano copper was used to evaluate the antioxidant and antibacterial activity against urinary tract infections pathogens. The resulting constructed nanoparticles were characterized by using ultraviolet spectroscopy absorbance around 370 nm. Scanning electron microscopy results showed the distribution of nanoparticles and particles sizes are foun...
Source: Artificial Cells, Nanomedicine and Biotechnology - September 15, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Study of tissue engineered vascularised oral mucosa-like structures based on ACVM-0.25% HLC-I scaffold in  vitro and in vivo.
CONCLUSION: The tissue-engineered vascularised complexes constructed by HGFs, HGECs, VEC-like cells and ACVM-0.25% HLC-I has good biocompatibility and considerable strength. PMID: 32924619 [PubMed - as supplied by publisher] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - September 15, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Resveratrol promotes secretion of wound healing related growth factors of mesenchymal stem cells originated from  adult and fetal tissues.
This study aims to investigate the effect of RV on secretion of wound healing related growth factors. We isolated and characterised MSCs from wharton's jelly (WJ), amniotic membrane (AM), and adipose tissue. We treated MSCs with serum deprived medium (SDM) supplemented with RV at 0.1 µM, 0.5 µM, 0.8 µM concentration. Our study revealed that RV at 0.1 µM was more effective to increase cell proliferation rate. Resveratrol at 0.1 µM promoted EGF, HGF, PDGF, and TGF-β1 secretion from MSCs. AD-MSCs showed the greatest response to RV stimulation in the term of...
Source: Artificial Cells, Nanomedicine and Biotechnology - September 11, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Micronized curcumin fabricated by supercritical CO2 to improve antibacterial activity against Pseudomonas aeruginosa.
Authors: Xue B, Huang J, Zhang H, Li B, Xu M, Zhang Y, Xie M, Li X Abstract Curcumin (CM) is a natural polyphenolic compound with multiple biomedical functions. However, clinical applications face more challenges due to its low dissolution rate and poor bioavailability. Micronization is an effective strategy to overcome these drawbacks. Herein, CM nanoparticles (CM NPs, ∼300 nm) were fabricated using solution enhanced dispersion by supercritical CO2 (SEDS). The solubility of CM NPs was remarkably enhanced. Aim to study the effects of micronization on the biological functions of CM, we investigated the an...
Source: Artificial Cells, Nanomedicine and Biotechnology - September 10, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Near-infra-red fluorescent chitosan oligosaccharide lactate for targeted cancer imaging and photothermal therapy.
In this study, we developed a bifunctional near-infra-red (NIR) fluorescent conjugate consisting of chitosan oligosaccharide lactate (COL) and the ZW800-1 NIR fluorophore (COL-ZW). We demonstrate that this conjugate is easy to use and that it is an effective theranostic agent for fluorescence-guided photothermal treatment. The temperature of COL-ZW increased by 62.3 °C after NIR laser irradiation (1.1 W/cm2) for 5 min in HT-29 tumour-bearing mice. The HT-29 tumours targeted by COL-ZW showed a remarkable decrease in tumour volume until a week after photothermal treatment. These in vivo results ...
Source: Artificial Cells, Nanomedicine and Biotechnology - September 5, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Protective effects of isorhynchophylline against silicon-dioxide-induced lung injury in mice.
Authors: Qiu M, Yang Z, Bian M, Liu C, Zhao Y, Liu Q Abstract Inhalation of silicon dioxide (SD) results in pulmonary inflammatory responses and fibrosis. Isorhynchophylline (Isorhy) is the main alkaloid in the traditional Chinese herb Tripterygium wilfordii, which is reported to have anti-inflammatory activities in the nervous system. However, the effects of Isorhy on SD-induced pulmonary inflammation and fibrosis in mice are unknown. Male mice were exposed to a single dose of SD (2.5 mg/kg, intranasal inoculation) to induce pulmonary fibrosis (PF). The mice were woken up and immediately treated with Isorhy...
Source: Artificial Cells, Nanomedicine and Biotechnology - September 5, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

An overview of various treatment strategies, especially tissue engineering for damaged articular cartilage.
Authors: Rahmani Del Bakhshayesh A, Babaie S, Tayefi Nasrabadi H, Asadi N, Akbarzadeh A, Abedelahi A Abstract Many traditional procedures, including surgical methods such as microfracture of subchondral bone and soft tissue transplantation, have been widely used to treat damaged cartilage. However, there is still no definitive cure for cartilage defects. In recent decades, tissue engineering has raised hopes for the repair of defective cartilage. Different approaches are used for cartilage engineering, in which cells, scaffolds, and biological signals or growth factors may be used alone or in combination. Additiona...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 29, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Zinc oxide nanoparticles synthesised from the Vernonia amygdalina shows the anti-inflammatory and antinociceptive activities in the mice model.
In conclusion, the findings of this study proved the beneficial effects of zinc oxide nanoparticles from V. amygdalina against the different pain and inflammation-induced mice. Hence, it was clear that the zinc nanoparticles from V. amygdalina could be promising antinociceptive and anti-inflammatory agent in the future. PMID: 32815404 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - August 22, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Systemic and microvascular comparison of Lactated Ringer's solution, VIR-HBOC, and alpha-alpha crosslinked haemoglobin-based oxygen carrier in a rat 10% topload model.
Authors: Song BK, Light WR, Vandegriff KD, Tucker J, Nugent WH Abstract Medical support for traumatic haemorrhage is lacking for far-forward combat units. VIR-HBOC (haemoglobin-based oxygen carrier) is a novel biological therapeutic under development as a field-stable resuscitation fluid. HBOCs have a long history of complications, chief among them is vasoconstrictive hypertension, which must be resolved before efficacy testing. As such, VIR-HBOC was compared against Lactated Ringers (LRS; vehicle) and a cross-linked haemoglobin (ααHb; a known vasoactive HBOC) in a rat topload model. Twenty-three male, ...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 22, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The negative effects of obesity on heart, especially the electrophysiology of the heart.
Authors: Chen H, Wang X, Xiong C, Zou H Abstract Obesity is associated with ventricular arrhythmia and sudden cardiac death. Numerous studies have shown that obesity may have effects on the heart by affecting the ventricular re-polarisation (VR). As an effective detection method for VR the measurement of the QT interval has been extensively studied in obese patients (OP). This review aims to investigate the relationship between obesity and obesity-related diseases; including diabetes, hypertension and cardiovascular diseases (CVD). This review compares the advantages and disadvantages of different QT interval measu...
Source: Artificial Cells, Nanomedicine and Biotechnology - July 23, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

LINC00162 participates in the pathogenesis of diabetic nephropathy via modulating the miR-383/HDAC9 signalling pathway.
In this study, we aimed to explore the potential role of lncRNA LINC-00162 in the pathogenic process of DN. LncRNA microarray analysis, real-time PCR, IHC computational analysis and luciferase assay were performed to explore the regulatory relationship among LINC00162, miR-383 and HDAC9. There was an obvious difference between T2D + DN and T2D - DN patients in their levels of eGRF and albuminuria. A significant difference was observed between T2D + DN and T2D - DN groups in terms of their LINC00162 expression. In particular, LINC00162 and HDAC9 were highly expressed, ...
Source: Artificial Cells, Nanomedicine and Biotechnology - July 19, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Magnetically active pNIPAM nanosystems as temperature-sensitive biocompatible structures for controlled drug delivery.
Authors: Garcia-Pinel B, Ortega-Rodríguez A, Porras-Alcalá C, Cabeza L, Contreras-Cáceres R, Ortiz R, Díaz A, Moscoso A, Sarabia F, Prados J, López-Romero JM, Melguizo C Abstract Here, temperature-sensitive hybrid poly(N-isopropylacrylamide) (pNIPAM) nanosystems with magnetic response are synthesised and investigated for controlled release of 5-fluorouracil (5FU) and oxaliplatin (OXA). Initially, magnetic nanoparticles (@Fe3O4) are synthesised by co-precipitation approach and functionalised with acrylic acid (AA), 3-butenoic acid (3BA) or allylamine (AL) as comonomers. The thermo-...
Source: Artificial Cells, Nanomedicine and Biotechnology - July 16, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Osteoclastic activity was associated with the development of steroid-induced osteonecrosis of femoral head.
This study is focussed on evaluating and comparing two mediators of osteoclast, osteoprotegerin (OPG) and nuclear factor-κB ligand (RANKL), in plasma and tissue levels in patients with steroid-induced osteonecrosis of femoral head (SIONFH). Subjects were included in this cross-sectional case-control study in 2016. Bone histomorphology, immunohistochemistry, Western blotting, OPG and RANKL plasma levels, post-hoc statistical power and receiver-operating characteristic (ROC) curves were evaluated. Eighty-six patients diagnosed with SIONFH and 51 healthy subjects were included. OPG expression levels in bone samples incr...
Source: Artificial Cells, Nanomedicine and Biotechnology - July 16, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

A metabolomic study on the anti-depressive effects of two active components from Chrysanthemum morifolium.
Authors: Liu T, Zhou N, Xu R, Cao Y, Zhang Y, Liu Z, Zheng X, Feng W Abstract Chrysanthemum morifolium (Chr) is a traditional Chinese medicine (TCM) that has been used in the treatment of inflammation-linked diseases for hundreds of years. Naringenin (Nar) and apigenin (Api) are the major active components in aqueous extracts of C. morifolium. The aim of our study was to clarify the roles of Chr, Nar and Api in ameliorating depression-like behaviour induced by corticosterone. First, the behavioural and biochemical indicators closely related to depression were examined to evaluate the therapeutic effects of Chr/Nar/...
Source: Artificial Cells, Nanomedicine and Biotechnology - July 15, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Localised delivery of quercetin by thermo-sensitive PLGA-PEG-PLGA hydrogels for the treatment of brachial plexus avulsion.
In conclusion, the temperature-sensitive quercetin loaded PLGA-PEG-PLGA hydrogel sustained-release system can alleviate oxidative damage and inflammation in the spinal cord, increase neuron survival rate, and promote nerve regeneration and motor function recovery in rats with early BPA. The findings suggest that this drug-loaded hydrogel has potential applications in the clinical treatment of BPA. PMID: 32608269 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - July 3, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Correction.
Authors: PMID: 32597689 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - July 2, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Identification of potential key pathways, genes and circulating markers in the development of intracranial aneurysm based on weighted gene co-expression network analysis.
Conclusions: Potential IA-related key pathways, genes and circulating markers were identified for predicting IA rupture by WGCNA analysis. PMID: 32589050 [PubMed - as supplied by publisher] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - June 28, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

MicroRNA-155 acts as a diagnostic and prognostic biomarker for oesophageal squamous cell carcinoma.
In this study, we found that miR-155 was significantly increased in ESCC tissues compared with the paired adjacent tissues and healthy normal controls (p 
Source: Artificial Cells, Nanomedicine and Biotechnology - June 25, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

LINC02163 promotes colorectal cancer progression via miR-511-3p/AKT3 axis.
Authors: Ma J, Zhang L, Shang A, Song H, Huo J, Zhang M, Jiang L Abstract Long non-coding RNAs and microRNAs are functional regulators in tumour progression. Herein, we revealed the level LINC02163 was up-regulated in CRC tissues and cell lines, and the expression of LINC02163 negatively correlated with prognosis of CRC patients. Functional experiments demonstrated knockdown of LINC02163 significantly attenuated CRC cells proliferation and metastasis. Mechanism analysis showed miR-511-3p could bind LINC02163 and AKT3, and the expressional level of miR-511-3p negatively correlated with the abundance of LINC02163 and...
Source: Artificial Cells, Nanomedicine and Biotechnology - June 13, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The enhanced treatment efficacy of invasive brain glioma by dual-targeted artemether plus paclitaxel micelles.
In this study, artemether and paclitaxel were used as two agents for tumour suppression. Two functional materials were synthesised and modified on the surface of the micelle as targeting molecules. The addition of two functional materials confers the ability of the micelles to effectively cross the blood-brain barrier (BBB) and then target the glioma cells. Thus, this dual-targeted delivery system allows the drug to play a better role in inhibiting tumour invasion and vasculogenic mimicry (VM) channels. In this paper, the anticancer effects of dual-targeted artemether plus paclitaxel micelles on glioma U87 cells were studi...
Source: Artificial Cells, Nanomedicine and Biotechnology - June 13, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Protocatechuic acid inhibits proliferation, migration and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes.
In conclusion, the results indicated that PCA exhibited an inhibitory effect on RA-FLSs via inhibiting the NF-κB and Akt/mTOR signalling pathways. These findings supported the concept that PCA might be a therapeutic agent for RA treatment. PMID: 32510258 [PubMed - as supplied by publisher] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - June 9, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Whether miR-4293 rs12220909 variant affects cancer susceptibility: evidence from 11255 subjects.
Conclusion: In summary, our meta-analysis chase down heterozygote rs12220909 polymorphism of miR-4293 is a protective factor to cancer initiation, especially for lung cancer. PMID: 32496828 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - June 6, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Diallyl disulphide-loaded spherical gold nanoparticles and acorn-like silver nanoparticles synthesised using onion extract: catalytic activity and cytotoxicity.
Authors: Lee YJ, Cha SH, Kim H, Choi SE, Cho S, Park Y Abstract Onion (Allium cepa) extract was used for the green synthesis of gold and silver nanoparticles. Each colloidal solution exhibited surface plasmon resonance, with a peak at 532 nm for gold nanoparticles and 391 nm for silver nanoparticles. Microscopic results confirmed the presence of spherical shapes. The X-ray diffraction pattern demonstrated a face-centered cubic structure. Both nanoparticles had negative zeta potentials and retained colloidal stability in cell culture medium. Catalytic applications were evaluated for 4-nitrophenol reduc...
Source: Artificial Cells, Nanomedicine and Biotechnology - June 6, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Anti-hepatoma effect of taccalonolide A through suppression of sonic hedgehog pathway.
Authors: Tian H, He Z Abstract Taccalonolide A has been reported to have anti-tumour efficiency. However, the underlying mechanism for taccalonolides A therapy of hepatocellular carcinoma (HCC) is still obscure. Cell viability was evaluated by cell counting kit-8 (CCK-8) assay. Apoptosis was determined by flow cytometry. Protein expression of B cell lymphoma (Bcl-2), Bcl-2 associated X (Bax), sonic hedgehog (Shh), Smoothened (Smo) and Gli family zinc finger 1 (Gli1) was analyzed by western blot. The expression of Shh, Smo and Gli1 mRNA was determined using quantitative real-time polymerase chain reaction (qRT-PCR)....
Source: Artificial Cells, Nanomedicine and Biotechnology - June 6, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Concentrated growth factor promotes gingival regeneration through the AKT/Wnt/ β-catenin and YAP signaling pathways.
Concentrated growth factor promotes gingival regeneration through the AKT/Wnt/β-catenin and YAP signaling pathways. Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):920-932 Authors: Qi L, Liu L, Hu Y, Li J, Li J, Cao N, Zhu F, Shi C, Zhang L Abstract Although concentrated growth factor (CGF) is known to promote gingival regeneration and improve the outcomes of clinical treatment, the mechanisms underlying its effects remain unknown. Therefore, this study aimed to elucidate the effects of CGF on gingival thickening. To this end, gingival mesenchymal stem cells (GMSCs) were treated with different conc...
Source: Artificial Cells, Nanomedicine and Biotechnology - June 6, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Construction of immune-related risk signature for uveal melanoma.
Authors: Zhang Z, Ni Y, Chen G, Wei Y, Peng M, Zhang S Abstract Uveal melanoma (UM) is the most frequent primary ocular tumour among adults. Here, we aimed to establish the immune cell-based signature to predict the overall survival (OS) of UM patients. The mRNA profile and matched clinical records of 80 UM patients were downloaded from The Cancer Genome Atlas (TCGA) database. CIBERSORT was used to verify the immune cell types of individuals. The univariate analysis found the CD8+ T cell, monocyte, CD4+ memory T cell (resting) and mast cell (resting) were significantly associated with the OS of UM patients. Subsequ...
Source: Artificial Cells, Nanomedicine and Biotechnology - June 6, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

RDM1 plays an oncogenic role in human ovarian carcinoma cells.
Authors: Tong L, Cao W, Sheng J, Zhu E, Yu Y, Zhong T, Chen Y, Wang L Abstract Ovarian cancer is one of the deadliest gynecological cancer, with a low overall 5-year survival rate. RDM1, RAD52 motif-containing protein 1, is sensitive to cisplatin, a common chemotherapy drug and it has an important role inDNA damage repair pathway. Until now, the effect of RDM1 in ovarian cancer is undiscovered. Here, clinical data shows that the tumour tissues of ovarian carcinoma patients with higher mRNA and protein expression of RDM1. Knockdown of RDM1 in ovarian carcinoma cells reduces cell proliferation and promotes apoptosis,...
Source: Artificial Cells, Nanomedicine and Biotechnology - June 6, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Biosynthesis of antioxidative enzymes and polyphenolics content in calli cultures of Prunella vulgaris L. in response to auxins and cytokinins.
This study will contribute in the development of cell culture in fermenter and synthesis of antioxidant secondary metabolites for commercial uses. PMID: 32490684 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - June 5, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Effect of gastric bypass on BMI and lipid metabolism in type 2 diabetes mellitus.
Authors: Zhang C, Cai W, Zhao H, Zhu M, Cui J, Sun Z Abstract To compare the clinical efficacy of gastric bypass in obese patients with T2DM with different BMI. Serum leptin, adiponectin, triglyceride (TG), cholesterol (CHOL) were measured as the indicators to show clinical efficacy after laparoscopic gastric bypass surgery (LRYGB). For patients with high BMI and patients with low BMI, the therapeutic effect of LRYGB surgical diabetes is more significant. The postoperative remission rate of diabetes in the high BMI group was not correlated with the preoperative lipid metabolism index but was positively correlated w...
Source: Artificial Cells, Nanomedicine and Biotechnology - June 3, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Enhanced sensitivity and efficiency of detection of Staphylococcus aureus based on modified magnetic nanoparticles by photometric systems.
In this study, a nano-biosensor based on iron nanoparticles (MNPs) was designed to detect S. aureus. MNPs were synthesized and conjugated to Biosensors. Then S. aureus was lysed and nano-biosensor (MNP-TiO2-AP-SMCC-Biosensors) was added to the lysed bacteria. After bonding the bacterial genome to the nano-biosensor, MNPs were separated by a magnet. Bacterial DNA was released from the surface of nano-biosensor and researched by Nano-drop spectrophotometry. The results of SEM and DLS revealed that the size of MNPs was 20-25 nm which increased to 38-43 nm after modification and addition of biosensors. The design...
Source: Artificial Cells, Nanomedicine and Biotechnology - June 3, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Evaluation of relationship between SPON1 gene and genetic susceptibility of postmenopausal osteoporosis.
Conclusions: Our results provide further evidence to support the important role for the SPON1 gene in the aetiology of PMOP, adding to the current understanding of the susceptibility to osteoporosis. PMID: 32484721 [PubMed - as supplied by publisher] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - June 3, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The P50 value detected by the oxygenation-dissociation analyser and blood gas analyser.
Authors: Chu Z, Wang Y, You G, Wang Q, Ma N, Li B, Zhao L, Zhou H Abstract Oxygen tension at 50% haemoglobin saturation (P50), which reflects the degree of peripheral oxygen offloading and tissue oxygenation, plays an important role in the diagnosis and treatment of disease, as well as in transfusion research. Blood gas analysers are commonly used in clinical and obtain P50 values through complex calculations and analysis. Oxygenation-dissociation analysers are specially designed to record the oxygen dissociation curves and obtain P50 values of whole blood, red blood cells (RBCs), and stroma-free haemoglobin. Howev...
Source: Artificial Cells, Nanomedicine and Biotechnology - May 30, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

A systematic review and meta-analysis of long noncoding RNA linc-UBC1 expression and prognosis and clinicopathological phenotypes in human cancers.
Authors: Zhang X, Xie H, Luo M, Liang D, Lao W, Ma W, Lin Y Abstract According to previous studies, linc-UBC1 is abnormally expressed in various human tumours. Nonetheless, the clinical significance and mechanism of linc-UBC1 in cancer remains unclear. In our present analysis, we wanted to explore the specific role of linc-UBC1 in malignant tumours by integrating all of the relevant literature and subsequently elucidating the relationship between linc-UBC1 expression level and clinical characteristics of cancers. An elaborate database search of PubMed, Embase, Wanfang Data, Web of Science, Ovid, Medline, Cochrane L...
Source: Artificial Cells, Nanomedicine and Biotechnology - May 30, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Everestmab, a novel long-acting GLP-1/anti GLP-1R nanobody fusion protein, exerts potent anti-diabetic effects.
Authors: Pan H, Su Y, Xie Y, Wang W, Qiu W, Chen W, Lu W, Lu Z, Wang W, Shang A Abstract In the present study, a novel single domain antibody (sdAb) fusion protein, named everestmab, composing of a mutated GLP-1(A8G) fused to the tandem bispecific humanized GLP-1R-targeting and albumin-binding nanobodies was designed and characterized for the therapies for type 2 diabetes mellitus (T2DM). Surface plasmon resonance (SPR) measurements demonstrated everestmab associates with serum albumins of rat and monkey species with high affinity, and tends to be cross-reactive with rat and monkey species. In vitro GLP-1R binding ...
Source: Artificial Cells, Nanomedicine and Biotechnology - May 30, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The survival benefit of adjuvant radiotherapy for pathological T4N2M0 colon cancer in the Modern Chemotherapy Era: evidence from the SEER database 2004-2015.
Authors: Huang Y, Gu X, Ge K, Fu G, Chu J, Wei W Abstract Neoadjuvant chemoradiotherapy has been established as the standard treatment for patients with locally advanced rectal cancer. However, the role of radiotherapy (RT) has not been fully confirmed in advanced colon cancer (LACC). We postulated that patients with pathological T4N2 locally advanced colon cancer would benefit more from RT. 6715 pT4N2M0 colon cancer patients were included in the Surveillance, Epidemiology, and End Results (SEER) database. The primary endpoints were 5-year overall survival (OS) and cancer-specific survival (CSS). Propensity score m...
Source: Artificial Cells, Nanomedicine and Biotechnology - May 29, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

An emerging technology in lipid research for targeting hydrophilic drugs to the skin in the treatment of hyperpigmentation disorders: kojic acid-solid lipid nanoparticles.
Authors: Khezri K, Saeedi M, Morteza-Semnani K, Akbari J, Rostamkalaei SS Abstract Kojic acid (KA) as tyrosinase inhibitor shows insufficient skin penetration and several adverse events due topical administration. KA solid lipid nanoparticles (KA-SLNs) were prepared using high speed homogenisation followed by ultra-probe sonication method for improve its effectiveness.KA-SLNs was optimised by Glyceryl mono-stearate (GMS) and Cholesterol (Chol) as lipid excipients and span 60 (SP 60) and Tween 20 (Tw 20) as co-emulsifiers (particle size 156.97 ± 7.15 nm, encapsulation efficiency 59.02&thi...
Source: Artificial Cells, Nanomedicine and Biotechnology - May 29, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Anti-tumour activity of zinc ionophore pyrithione in human ovarian cancer cells through inhibition of proliferation and migration and promotion of lysosome-mitochondrial apoptosis.
In conclusion, our work suggests that ZPT combined with Zinc could inhibit proliferation, migration, invasion, and promote apoptosis by trigger the lysosome-mitochondrial apoptosis pathway in ovarian carcinoma. PMID: 32456481 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - May 29, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Statement of Retraction.
Authors: PMID: 32441139 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - May 24, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Statement of Retraction.
Authors: PMID: 32441141 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - May 24, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Statement of Retraction.
Authors: PMID: 32441145 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - May 24, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Statement of Retraction.
Authors: PMID: 32441146 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - May 24, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Statement of Retraction.
Authors: PMID: 32441161 [PubMed - in process] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - May 24, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Pseudomonas aeruginosa synthesized silver nanoparticles inhibit cell proliferation and induce ROS mediated apoptosis in thyroid cancer cell line (TPC1).
Authors: Yang J, Wang Q, Wang C, Yang R, Ahmed M, Kumaran S, Velu P, Li B Abstract We used cell-free culture filtrate of Pseudomonas aeruginosa as a reducing mediator of AgNO3 to silvernanoparticles (AgNPs) and possibly used as a potential anticancer agent against thyroid cancer cells (TPC1). The bio-generation of AgNPs was firmly established by taking a UV spectrum at 380-500 nm wavelength. The Fourier transform spectrum analysis reveals the association of alcohol, phenol and aromatic functional groups with P. aeruginosa synthesized AgNPs (Ps-AgNPs). By observing under transmission electron microscopy (TEM)...
Source: Artificial Cells, Nanomedicine and Biotechnology - May 21, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Serum levels of PTEN mRNA in colorectal cancer: a case-control study.
Authors: Yan Y, Du X, Xia S, Li S, Teng D, Hu S, Wang Y, Li R Abstract Background: The purpose of this study was to identify the expression of phosphatase and tensin homolog (PTEN) and its diagnostic value in colorectal cancer (CRC).Methods: The expression level of serum PTEN at mRNA and protein level were determined using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analyses, respectively. Chi-square test was employed to explore the relationship between PTEN expression and clinical features of CRC patients. The receiver operating characteristics (ROC) curve was established to evaluat...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 24, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Intracellular synthesis of gold nanoparticles by Gluconacetobacter liquefaciens for delivery of peptide CopA3 and ginsenoside and anti-inflammatory effect on lipopolysaccharide-activated macrophages.
Authors: Liu Y, Perumalsamy H, Kang CH, Kim SH, Hwang JS, Koh SC, Yi TH, Kim YJ Abstract Probiotic Gluconacetobacter strains are intestinal microbes with beneficial effects on human health. Recently, researchers have used these strains to biosynthesize metal and non-metal nanoparticles for treating various chronic diseases. Despite their importance in nanotechnology, gold nanoparticles (AuNPs) biosynthesized by Gluconacetobacter species have not been clearly identified for treating inflammation and inflammation-associated diseases. While ginsenoside CK has strong pharmaceutical activity, it also has strong cytotoxi...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 21, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Natural polymeric nanoparticles as a non-invasive probe for mesenchymal stem cell labelling.
In this study, chitosan-alginate nanoparticles were synthesized as a clinical Dotarem® carrier for decreased t1-weighted. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and Fourier-transform infrared spectroscopy (FTIR) were utilized for nanoprobe characterization and ICP analysis was performed for Gd3+ concentration measurement. The results illustrate that nanoprobes with spherical shape and with a size of 80 nm without any aggregation were obtained. Relaxivity results suggest that r1 in the phantom was 12.8 mM-1s-1 per Gd3+ ion, which is 3.5 t...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 17, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Expression of concern: Involvement of NORAD/miR-608/STAT3 axis in carcinostasis effects of physcion 8-O-b-glucopyranoside on ovarian cancer cells.
Authors: PMID: 32285690 [PubMed] (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - April 16, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research