Liposomes for drug delivery: review of vesicular composition, factors affecting drug release and drug loading in liposomes
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):428-440. doi: 10.1080/21691401.2023.2247036.ABSTRACTLiposomes are considered among the most versatile and advanced nanoparticle delivery systems used to target drugs to specific cells and tissues. Structurally, liposomes are sphere-like vesicles of phospholipid molecules that are surrounded by equal number of aqueous compartments. The spherical shell encapsulates an aqueous interior which contains substances such as peptides and proteins, hormones, enzymes, antibiotics, antifungal and anticancer agents. This structural property of liposomes makes it an important nano-carrier f...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 18, 2023 Category: Biotechnology Authors: Shantanu Pande Source Type: research

Anticancer potentials of metformin loaded coconut oil nanoemulsion on MCF-7, HepG2 and HCT-116 cell lines
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):419-427. doi: 10.1080/21691401.2023.2246145.ABSTRACTIn order to load metformin in a nano formula and evaluate the produced nano form towards cancer cells, metformin was loaded on natural carrier coconut oil. The formed metformin-loaded coconut oil nanoemulsion was characterized by Zeta potential, particle size, drug content, drug release, and drug stability. The formed nanoemulsion was evaluated towards MCF-7, HepG2, and HCT-116 cell lines. Cell cycle analysis and apoptosis mechanism were studied. The nanoemulsion was created using deionized water, 1.5% Span 20, 1.5% Tween 80,...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 17, 2023 Category: Biotechnology Authors: Hadil Faris Alotaibi El-Sayed Khafagy Amr Selim Abu Lila Haifa F Alotaibe Serag Eldin Elbehairi Ashwag S Alanazi Mohammad Y Alfaifi Jawaher Abdullah Alamoudi Sarah Salem Alamrani Fatma Alzahraa Mokhtar Source Type: research

Generation of a novel < em > ex-vivo < /em > model to study re-endothelialization
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):408-416. doi: 10.1080/21691401.2023.2245456.ABSTRACTEndothelial dysfunction initiates the pathogenesis of a myriad of cardiovascular diseases, yet the precise underlying mechanisms remain unclear. Current model utilises mechanical denudation of arteries resulting in an arterial-injury model with onset of intimal hyperplasia (IH). Our study shows that 5 min enzymatic denudation of human umbilical artery (hUA) lumen at 37 °C efficiently denudes hUA while maintaining vessel integrity without significantly increase intima-media thickness after 7 days in culture. This ex-vivo mode...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 16, 2023 Category: Biotechnology Authors: Siti Sarah Azman Muhammad Dain Yazid Nur Azurah Abdul Ghani Raja Zahratul Azma Raja Sabudin Mohd Ramzisham Abdul Rahman Nadiah Sulaiman Source Type: research

Correction
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):417-418. doi: 10.1080/21691401.2023.2245267.NO ABSTRACTPMID:37577808 | DOI:10.1080/21691401.2023.2245267 (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - August 14, 2023 Category: Biotechnology Source Type: research

An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):371-383. doi: 10.1080/21691401.2023.2239274.ABSTRACTElectrical characteristics of living cells have been proven to reveal important details about their internal structure, charge distribution and composition changes in the cell membrane, as well as the extracellular context. An impedance flow cytometry is a common approach to determine the electrical properties of a cell, having the advantage of label-free and high throughput. However, the current techniques are complex and costly for the fabrication process. For that reason, we introduce an integrated dual microneedle-microch...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 7, 2023 Category: Biotechnology Authors: Muhammad Asraf Mansor Mohd Ridzuan Ahmad Michal Petr ů Seyed Saeid Rahimian Koloor Source Type: research

IgG antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancer
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):384-396. doi: 10.1080/21691401.2023.2242419.ABSTRACTVincamine, a natural chemical, was used as a reducing agent in the synthesis of IgG antibodies mediated biogenic gold nanoparticles (IgGAuNPs). Eventually, the synthesised IgGAuNPs were bioconjugated with the chemotherapeutic drug methotrexate (MTX-IgGAuNPs). The IgG isotype can target cancer cells through polymorphic Fc gamma receptors (FcγRs) and have therapeutic effects. They can restrict cell division by inhibiting different intracellular signal transduction pathways and activating NK cells and macrophages through antibo...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 7, 2023 Category: Biotechnology Authors: Asad Syed Abu Baker Mohamed Mohany Abdallah M Elgorban Mohd Sajid Khan Salim S Al-Rejaie Source Type: research

An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):371-383. doi: 10.1080/21691401.2023.2239274.ABSTRACTElectrical characteristics of living cells have been proven to reveal important details about their internal structure, charge distribution and composition changes in the cell membrane, as well as the extracellular context. An impedance flow cytometry is a common approach to determine the electrical properties of a cell, having the advantage of label-free and high throughput. However, the current techniques are complex and costly for the fabrication process. For that reason, we introduce an integrated dual microneedle-microch...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 7, 2023 Category: Biotechnology Authors: Muhammad Asraf Mansor Mohd Ridzuan Ahmad Michal Petr ů Seyed Saeid Rahimian Koloor Source Type: research

IgG antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancer
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):384-396. doi: 10.1080/21691401.2023.2242419.ABSTRACTVincamine, a natural chemical, was used as a reducing agent in the synthesis of IgG antibodies mediated biogenic gold nanoparticles (IgGAuNPs). Eventually, the synthesised IgGAuNPs were bioconjugated with the chemotherapeutic drug methotrexate (MTX-IgGAuNPs). The IgG isotype can target cancer cells through polymorphic Fc gamma receptors (FcγRs) and have therapeutic effects. They can restrict cell division by inhibiting different intracellular signal transduction pathways and activating NK cells and macrophages through antibo...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 7, 2023 Category: Biotechnology Authors: Asad Syed Abu Baker Mohamed Mohany Abdallah M Elgorban Mohd Sajid Khan Salim S Al-Rejaie Source Type: research

An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):371-383. doi: 10.1080/21691401.2023.2239274.ABSTRACTElectrical characteristics of living cells have been proven to reveal important details about their internal structure, charge distribution and composition changes in the cell membrane, as well as the extracellular context. An impedance flow cytometry is a common approach to determine the electrical properties of a cell, having the advantage of label-free and high throughput. However, the current techniques are complex and costly for the fabrication process. For that reason, we introduce an integrated dual microneedle-microch...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 7, 2023 Category: Biotechnology Authors: Muhammad Asraf Mansor Mohd Ridzuan Ahmad Michal Petr ů Seyed Saeid Rahimian Koloor Source Type: research

IgG antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancer
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):384-396. doi: 10.1080/21691401.2023.2242419.ABSTRACTVincamine, a natural chemical, was used as a reducing agent in the synthesis of IgG antibodies mediated biogenic gold nanoparticles (IgGAuNPs). Eventually, the synthesised IgGAuNPs were bioconjugated with the chemotherapeutic drug methotrexate (MTX-IgGAuNPs). The IgG isotype can target cancer cells through polymorphic Fc gamma receptors (FcγRs) and have therapeutic effects. They can restrict cell division by inhibiting different intracellular signal transduction pathways and activating NK cells and macrophages through antibo...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 7, 2023 Category: Biotechnology Authors: Asad Syed Abu Baker Mohamed Mohany Abdallah M Elgorban Mohd Sajid Khan Salim S Al-Rejaie Source Type: research

An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):371-383. doi: 10.1080/21691401.2023.2239274.ABSTRACTElectrical characteristics of living cells have been proven to reveal important details about their internal structure, charge distribution and composition changes in the cell membrane, as well as the extracellular context. An impedance flow cytometry is a common approach to determine the electrical properties of a cell, having the advantage of label-free and high throughput. However, the current techniques are complex and costly for the fabrication process. For that reason, we introduce an integrated dual microneedle-microch...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 7, 2023 Category: Biotechnology Authors: Muhammad Asraf Mansor Mohd Ridzuan Ahmad Michal Petr ů Seyed Saeid Rahimian Koloor Source Type: research

IgG antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancer
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):384-396. doi: 10.1080/21691401.2023.2242419.ABSTRACTVincamine, a natural chemical, was used as a reducing agent in the synthesis of IgG antibodies mediated biogenic gold nanoparticles (IgGAuNPs). Eventually, the synthesised IgGAuNPs were bioconjugated with the chemotherapeutic drug methotrexate (MTX-IgGAuNPs). The IgG isotype can target cancer cells through polymorphic Fc gamma receptors (FcγRs) and have therapeutic effects. They can restrict cell division by inhibiting different intracellular signal transduction pathways and activating NK cells and macrophages through antibo...
Source: Artificial Cells, Nanomedicine and Biotechnology - August 7, 2023 Category: Biotechnology Authors: Asad Syed Abu Baker Mohamed Mohany Abdallah M Elgorban Mohd Sajid Khan Salim S Al-Rejaie Source Type: research