Microwave-assisted single step cinnamic acid derivatization to mimic vanillin isomer and their cytotoxic potential.
CONCLUSIONS: In conclusion, the present study gives a very reliable and simple method for isolation of HMB and cinnamic acid modification that efficiently reduces the reaction time and proves to be friendly with environment. PMID: 31613725 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 15, 2019 Category: Biotechnology Authors: Mishra S, Singh S, Ali A, Gupta AC, Shanker K, Bawankule DU, Luqman S Tags: Curr Pharm Biotechnol Source Type: research

Recent advances in inhaled formulations and pulmonary insulin delivery systems.
Abstract Insulin (INS) therapy played a great role for patients in type 1 and type 2 diabetes to regulate blood glucose levels. Although hypodermic injection was commonly used for insulin delivery, it had some disadvantages such as pain, needle phobia and the risk of infection. Therefore, pulmonary insulin delivery had been developed as an alternative method to overcome the therapeutic challenges in recent years since pulmonary insulin administration showed great improvements in rapid action and circumvention of first-pass hepatic metabolism. This review described the most recent developments of pulmonary insulin ...
Source: Current Pharmaceutical Biotechnology - October 11, 2019 Category: Biotechnology Authors: Liu H, Shan X, Yu J, Li X, Hu L Tags: Curr Pharm Biotechnol Source Type: research

Inhibitory effect of sesamolin on melanogenesis in B16F10 cells by in vitro and in silico analysis.
CONCLUSION: Sesamolin is a good candidate for melanogenic inhibition. However, sesamolin was predicted as a weak sensitizer by Derek EC3 prediction. It is necessary to confirm the safety of sesamolin as a cosmetic material in a biological skin toxicity experiment. PMID: 31612825 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 11, 2019 Category: Biotechnology Authors: Baek SH, Kang MG, Parka D Tags: Curr Pharm Biotechnol Source Type: research

Antimicrobial Peptides: A Promising Avenue For Human Healthcare.
Abstract Antimicrobial drugs resistance microbes have been observed worldwide and therefore alternatively development of antimicrobial peptides has gained interest with potential advantages for human healthcare. Enormous progress has been made in the development of antimicrobial peptide during the last decade. This review provides recent information on current status of antimicrobial peptide development, their applications and future perspective in human therapies. PMID: 31612826 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 11, 2019 Category: Biotechnology Authors: Bhopale GM Tags: Curr Pharm Biotechnol Source Type: research

High dietary fat modulates neurobehavioural but not oxidative and neurochemical effects of lopinavir/ritonavir in mice.
CONCLUSION: In conclusion, the study shows that while dietary composition may alter the neurobehavioural responses to lopinavir/ritonavir in healthy mice, its effects on oxidative status, L-glutamate and acetylcholinesterase activity remains relatively unaffected. PMID: 31612827 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 11, 2019 Category: Biotechnology Authors: O J O, O I A, O J A, E A F, A T O, A Y O Tags: Curr Pharm Biotechnol Source Type: research

Healthcare-Associated Infections: Antibiotic Poly-therapies, Antibiotic Prophylaxis and Appropriate Policy for the Risk Management to Fight Adverse Events.
Abstract PMID: 31592746 [PubMed - in process] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 10, 2019 Category: Biotechnology Authors: Fineschi V Tags: Curr Pharm Biotechnol Source Type: research

Integrative Exome Sequencing Analysis in Castration-Resistant Prostate Cancer in Chinese Population.
CONCLUSION: we speculate that MST1 would be a new potential target for the treatment of CRPC by regulating of Hippo signaling pathway. The results provided an important clue to the molecular mechanism of CRPC. PMID: 31580249 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 3, 2019 Category: Biotechnology Authors: Hao L, Li H, Zhang S, Yang Y, Xu Z, Zhang Y, Liu Z Tags: Curr Pharm Biotechnol Source Type: research

Relationship between SNP rs1764391 and Susceptibility, Risk Factors, Gene-environment Interactions of Acute Myocardial Infarction in Guangxi Han Chinese Population.
This study aimed to examine the association between SNP rs1764391 of Cx37 and disease-susceptibility, several risk factors, gene-environment interactions of AMI in Guangxi Han Chinese. METHODS: In this study, 344 healthy controls and 344 AMI patients of Han Chinese were enrolled. The TaqMan assay was implemented to confirm the genotypes. Differences in genotype and allele frequencies of SNP rs1764391 were compared in both the AMI and control groups. RESULTS: Significant differences in TT genotype frequencies of SNP rs1764391 between the AMI and control groups were detected (P
Source: Current Pharmaceutical Biotechnology - October 3, 2019 Category: Biotechnology Authors: Li J, Qin R, Wang W, Huang Z, Huang DL, Li T, Wang F, Zeng XT, Sun ZY, Liu XF, Huang F, Guo T Tags: Curr Pharm Biotechnol Source Type: research

Multivariate optimization of the refolding process of an incorrectly folded Fc-fusion protein in cell culture broth.
CONCLUSION: The refolding process increases the fraction of active protein in original broth reducing the burden of downstream purification steps markedly. PMID: 31577202 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 2, 2019 Category: Biotechnology Authors: Behrouz H, Molavi B, Tavakoli A, Askari M, Maleknia S, Mahboudi F, Khodadadian M Tags: Curr Pharm Biotechnol Source Type: research

Characterizations and fibrinolytic activity of serine protease from Bacillus subtilis C10.
CONCLUSION: Results from the present study suggested that enzyme obtained from B. subtilis C10 could be a serine protease. B. subtilis C10 could be of potential use for cost-effective production of fibrinolytic protease that could be useful for medical applications. PMID: 31577203 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 2, 2019 Category: Biotechnology Authors: Thu NTA, Khue NTM, Huy ND, Tien NQD, Loc NH Tags: Curr Pharm Biotechnol Source Type: research

Identification of antibacterial molecule(s) from animals living in polluted environments.
CONCLUSION: The results revealed that python that feed on germ-infested rodents and water monitor lizards that feed on rotten organic waste possess antibacterial activity in a heat-sensitive manner and several peptides were identified through LC-MS. We hope that the discovery of antibacterial activity in the sera of animals living in polluted environments will stimulate research in finding antibacterial agents from unusual sources as this has potential for the development of novel strategies in the control of infectious diseases. PMID: 31577204 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 2, 2019 Category: Biotechnology Authors: Winnie FYM, Siddiqui R, Sagathevan K, Khan NA Tags: Curr Pharm Biotechnol Source Type: research

Synergistic antibacterial efficacy of biogenic synthesized silver nanoparticles using Ajuga bractosa with standard antibiotics: a study against bacterial pathogens.
CONCLUSION: It has been concluded that green synthesis is an innovative technology in which natural products are used against infectious pathogens. Current research shows the significant antibacterial effect against infectious agents. PMID: 31573882 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 1, 2019 Category: Biotechnology Authors: Nazer S, Andleeb S, Ali S, Gulzar N, Iqbal T, Khan MAR, Raza A Tags: Curr Pharm Biotechnol Source Type: research

RP-HPLC estimation of flavonol derivatives and phenolic acids in Capsicum annuum L. and their correlation with in vitro anti-inflammatory activity.
CONCLUSION: When correlation was established a strong positive correlation of 0.9 was observed, thereby postulating the role of quercetin and salicylic acid to be the active phytochemical of Capsicum annuum for its anti-inflammatory activity. Further research is needed to fully elucidate the phytochemicals accountable for its anti-inflammatory activity and to develop better herbal drug formulations. PMID: 31566132 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - September 30, 2019 Category: Biotechnology Authors: Iqbal Z, Ahmad A, Haque Z, Khan MS, Khan MS, Iqbal MS Tags: Curr Pharm Biotechnol Source Type: research

Lipid-based Vehicles for siRNA Delivery in Biomedical Field.
Abstract Genetic drugs have aroused much attention in the past twenty years. RNA interference (RNAi) offers novel insights into discovering potential gene functions and therapies targeting genetic diseases. Small interference RNA (siRNA), typically 21-23 nucleotides in length, can specifically degrade complementary mRNA. However, targeted delivery and controlled release of siRNA remain a great challenge. Considering the vast number of drug carriers, when compared with viral vectors, non-viral ones show reduced cytotoxicity and low production cost. Especially, lipid-based vehicles are more promising due to their hi...
Source: Current Pharmaceutical Biotechnology - September 24, 2019 Category: Biotechnology Authors: Li T, Huang L, Yang M Tags: Curr Pharm Biotechnol Source Type: research

Variances in the level of of COX-2 and iNOS in different grades of endometrial cancer.
CONCLUSIONS: Our findings suggest that changes in expression of COX-2 and iNOS may be potentially useful in predicting the progression of endometrial cancer and treatment effectiveness. PMID: 31533599 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - September 17, 2019 Category: Biotechnology Authors: Oplawski M, Dziobek K, Zmarzły N, Grabarek B, Kiełbasiński R, Kieszkowski P, Januszyk P, Talkowski K, Plewka A, Boroń D Tags: Curr Pharm Biotechnol Source Type: research

The In Vitro Anti-Cancer Activities of 17 βH-Neriifolin Isolated from Cerbera odollam and Its Binding Activity on Na+,K+-ATPase.
The In Vitro Anti-Cancer Activities of 17βH-Neriifolin Isolated from Cerbera odollam and Its Binding Activity on Na+,K+-ATPase. Curr Pharm Biotechnol. 2019 Sep 17;: Authors: Yunos NM, Osman A, Jauri MH, Sallehudin NJ, Mutalip SSM Abstract 17βH-neriifolin, a cardiac glycoside compound had been successfully isolated from Cerbera odollam leaves based on bioassay guided isolation procedure. The compound was tested against breast (MCF-7, T47D), colorectal (HT-29), ovarian (A2780, SKOV-3) and skin (A375) cancer cell lines that gave IC50 values ranged from 0.022 ± 0.0015 to 0.030 ± 0....
Source: Current Pharmaceutical Biotechnology - September 17, 2019 Category: Biotechnology Authors: Yunos NM, Osman A, Jauri MH, Sallehudin NJ, Mutalip SSM Tags: Curr Pharm Biotechnol Source Type: research

Evaluation of some biological applications of Pleurotus citrinopileatus and Boletus edulis fruiting bodies.
Abstract Mushrooms were deemed as a special delicacy by many countries. They identified as an essential cuisine due its bioactive ingredients and offer huge pivotal actions on personal fitness. Herein, we evaluated some biological aspects of the methanolic extracts of Pleurotus citrinopileatus and Boletus edulis fruiting bodies including; In vitro antimicrobial, potential anti-diabetic, antioxidant, potential anti-lipidemic and cytotoxic activity against different carcinoma and normal cell lines. B. edulis methanolic extract revealed superior antimicrobial and potential anti-diabetic activity. On the other hand, P...
Source: Current Pharmaceutical Biotechnology - September 4, 2019 Category: Biotechnology Authors: Younis AM, Abdel-Aziz MM, Yosri M Tags: Curr Pharm Biotechnol Source Type: research

In vitro Biological Estimation of [1,2,3]triazolo[4,5-d]pyrimidine Derivatives as Anti-breast Cancer Agent: DFT, QSAR and Docking Studies.
Abstract 20 sets of synthesized molecular compounds were considered as anti-breast cancer agent. The molecular descriptors which describe the microbial activities of the studied compounds were calculated using B3LYP/6-31+G* and obtained. The parameters obtained were EHOMO (eV), ELUMO (eV), dipole moment (Debye), log P, molecular weight (amu), HBA, HBD, Vol and Ovality were screened. The obtained calculated descriptors were used to develop QSAR model for prediction of experimental inhibition concentration (IC50) using SPSS and Gretl software packages for multiple linear regression (MLR) and MATLAB for artificial ne...
Source: Current Pharmaceutical Biotechnology - September 4, 2019 Category: Biotechnology Authors: Kolawole OA, Banjo S Tags: Curr Pharm Biotechnol Source Type: research

Antibacterial, antibiofilm, antihemolytic and antivirulence properties of low molecular weight chitooligosaccharides to Staphylococcus aureus.
CONCLUSION: The water solubility of chitosan limits its biological activity, the shortening the polymer size in the form of chitooligosaccharides (COS) becomes as alternate possibilities to maintain its activity. Hence, in the present study, different sizes of low molecular weight COS were used for the testing for antibacterial, antibiofilm and antivirulence properties against S. aureus. PMID: 31475895 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - September 2, 2019 Category: Biotechnology Authors: Khan F, Lee JW, Pham DTN, Kim YM Tags: Curr Pharm Biotechnol Source Type: research

A UPLC-MS/MS Assay for Simultaneous Determination of Two Antipsychotics and Two Antidepressants in Human Plasma and Its Application in Clinic.
Conclusions: The developed method was successfully used to simultaneous determination of FLU, ESC, RIS and ARI in patients' plasma, which is with great clinic value for individual therapy. PMID: 31470784 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - August 30, 2019 Category: Biotechnology Authors: Li H, Cheng X, Zhang D, Wang M, Dong W, Feng W Tags: Curr Pharm Biotechnol Source Type: research

Are Viruses and Parasites linked to Celiac Disease? A question that still has no definite answer.
Abstract Celiac disease is a complex autoimmune enteropathy of the small intestine that commonly occurs in genetically predisposed individuals due to intake of gluten and related proteins. Gluten consumption, duration of breast-feeding, various infections, especially frequent intestine infections, vaccinations and use of antibiotics can be linked to celiac disease. It is predicted that it affects 1% of the global population and its incidence rate is increasing. Celiac disease is closely related to Turner's syndrome, type I diabetes mellitus, Down's syndrome, and other autoimmune conditions and most people with the...
Source: Current Pharmaceutical Biotechnology - August 28, 2019 Category: Biotechnology Authors: Shariati A, Aslani HR, Shayesteh MRH, Taghipour A, Nasser A, Safari H, Alizade-Sani M, Dehghan A, Azimi T Tags: Curr Pharm Biotechnol Source Type: research

Coffea arabica bean extracts and vitamin C: A novel combination unleashes MCF-7 cell death.
Abstract BACKGROUND: Vitamin C (VC) is believed to enhance immunity and is regularly integrated as a supplementary agent during several treatments. OBJECTIVE: The green (GC) and roasted (RC) Coffee (Coffea arabica) aqueous extracts (0, 125, 250 and 500 μg/ml) combined with VC (50 μg/ml) were examined on the cancerous MCF-7 cell line and normal human lymphocytes. METHODS: Neutral red uptake assay, comet assay, immunocytochemical reactivity for protein expression and mRNA expression of apoptosis-related genes were done. RESULTS: A significant (P
Source: Current Pharmaceutical Biotechnology - August 22, 2019 Category: Biotechnology Authors: El-Garawani I, El-Nabi SH, El-Safey S, Elfiky M, Nafee E Tags: Curr Pharm Biotechnol Source Type: research

In-Vitro Anti-Proliferative, Apoptotic And Antioxidative Activities Of Medicinal Herb Kalonji (Nigella sativa).
CONCLUSION: It was observed that Kalonji extracts have ability to induce apoptosis and improve the antioxidant status of HeLa and HepG2 cells. They can also inhibit the proliferation and angiogenesis in both these cancer cell lines. PMID: 31433749 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - August 21, 2019 Category: Biotechnology Authors: Maqbool T, Awan SJ, Malik S, Hadi F, Shehzadi S, Tariq K Tags: Curr Pharm Biotechnol Source Type: research

Blood brain barrier permeability prediction using machine learning techniques: An update.
Abstract Blood Brain Barrier (BBB) is the collection of vessels of blood with special properties of permeability that allow a limited range of drug and compounds to pass through it. The BBB plays a vital role in maintaining balance between intracellular and extracellular environment for brain. Brain Capillary Endothelial Cells (BECs) act as vehicle for transport and the transport mechanisms across BBB involve active and passive diffusion of compounds. Efficient prediction models of BBB permeability can be vital at the preliminary stages of drug development. There have been persistent efforts in identifying the pre...
Source: Current Pharmaceutical Biotechnology - August 21, 2019 Category: Biotechnology Authors: Saxena D, Sharma A, Siddiqui MH, Kumar R Tags: Curr Pharm Biotechnol Source Type: research

Targeting clusterin induces apoptosis, reduces growth ability, invasion and mediates sensitivity to chemotherapy in human cancer in human osteosarcoma cells.
CONCLUSIONS: Overexpression of sCLU was significantly correlated with metastasis and chemosensitivity in osteosarcoma cells. The described sCLU-specific siRNA that can potently silence sCLU gene expression may thus prove valuable agents during antitumor therapy. PMID: 31433751 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - August 21, 2019 Category: Biotechnology Authors: Wang X, Yu Y, Zang L, Zhang P, Ma J, Chen D Tags: Curr Pharm Biotechnol Source Type: research

Ethnobotany and pharmacology of Pinus species growing naturally in Indian Himalayas: A plant review.
Abstract Pinus species found in the Indian Himalayas is among most commonly used medicinal plant in Northwestern. Nearly all parts of the plant especially needles are believed to possess medicinal and clinical values in traditional system of medicine be it Ayurvedic, Unani or Homeopathic systems of medicine. In these traditional systems of medicine, different part of pines are used to heal numerous diseases, including afflictions of the skin, eyes, throats and ears. Various parts of plant including needles are rich in various biologically active compounds such as thunbergol, 3-carene, cembrene, α-pinene, que...
Source: Current Pharmaceutical Biotechnology - August 19, 2019 Category: Biotechnology Authors: Singh L, Dixit P, Srivastava RP, Pandey S, Verma PC, Saxena G Tags: Curr Pharm Biotechnol Source Type: research

Market Analysis Of Medicinal Plants In India: Review.
Abstract There has been a significant growth in herbal drugs market around the world, over the last few decades due to growing awareness among people about the rising cost as well as side effects related to the use of synthetic drugs. Herbal medicine has been used in traditional medicinal systems around the world, especially India where the oldest systems of medicine namely Ayurveda, Siddha, and Unani make use of more than 90% plant-based formulations. India is rich in medicinal and herbal plants resources that provide for both the health care needs of rural India as well as the source of raw material for therapeu...
Source: Current Pharmaceutical Biotechnology - August 19, 2019 Category: Biotechnology Authors: Ravi S, Bharadvaja N Tags: Curr Pharm Biotechnol Source Type: research

Antibody Therapy for the Control of Viral Diseases: An Update.
Abstract The epidemiological impact of viral diseases combined with the emergence and reemergence of some viruses and the difficulties in identifying effective therapies have encouraged several studies to understand and develop new techniques for the prevention and treatment of viral infections. An example would be the development of immunotherapeutics and their recent application in the treatment and prevenction of viral infections through the use of conventional, bispecific, or multispecific monoclonal antibodies (mAbs). Currently, several immunotherapeutics for the treatment of different viral infections are in...
Source: Current Pharmaceutical Biotechnology - August 9, 2019 Category: Biotechnology Authors: Dibo M, Battocchio EC, Dos Santos Souza LM, da Silva MDV, Banin-Hirata BK, Miranda Sapla MM, Marinello P, Rocha SPD, Faccin-Galhardi LC Tags: Curr Pharm Biotechnol Source Type: research

Impact of the transfersome delivered human growth hormone on the dermal fibroblast cells.
In this study we used transdermal delivery of growth hormone-encapsulated transferosomes (F1 and F2) as antiaging strategy, with the resulting effects being subsequently evaluated. The size, distribution and zeta potential of the particles, together with the in vitro skin permeation and biological activity of the growth hormone loaded onto the transfersomes were measured. The data demonstrated that treatment of fibroblasts with encapsulated hGH increased cell migration, proliferation and collagen I and III gene expression. According to our results, the maximum amount of growth hormone that passes through the skin during a ...
Source: Current Pharmaceutical Biotechnology - August 9, 2019 Category: Biotechnology Authors: Azimi M, Khodabandeh M, Deezagi A, Rahimi F Tags: Curr Pharm Biotechnol Source Type: research

Changes In The Density Of Corneal Endothelial Cells In Elderly Diabetic Patients After Combined Phacovitrectomy And Ex-Press Glaucoma Implants.
Abstract Corneal endothelial cells (ECD) are characterized by limited regenerative potential, which is additionally impaired in patients with diabetes. This retrospective study included 27 patients aged 58.1±13.6, 16 female and 11 males, who underwent 23-gauge vitrectomy in combination with cataract surgery (phacovitrectomy) and further Ex-PRESS shunt implantation throughout 2013-2017 at St. Barbara Hospital in Sosnowiec, Poland. In our study we distinguished 4 periods: initial period; after phacovitrectomy and removal of oil tamponade; and 3 and 12 months after implantation of the Ex-PRESS shunt. Statistic...
Source: Current Pharmaceutical Biotechnology - August 8, 2019 Category: Biotechnology Authors: Lyssek-Boroń A, Krysik K, Kijonka M, Osuch M, Grabarek B, Dobrowolski D Tags: Curr Pharm Biotechnol Source Type: research

Bacillus thuringiensis toxins are cytotoxic to cervical cancer (HeLa) but not to breast cancer (MCF7) cells.
Conclusion; Bacillus thuringiensis toxins are able to have a dual cytotoxic action, on insects and on cancer cell lines. PMID: 31376817 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - August 2, 2019 Category: Biotechnology Authors: Mendoza-Almanza G, Rocha-Zavaleta L, Aguilar-Zacarías C, Ayala-Luján J, Olmos J Tags: Curr Pharm Biotechnol Source Type: research

Structure, genome, infection cycle and clinical manifestations associated with Human Papillomavirus.
Abstract A small, non-enveloped, obligatory parasite, Human papillomavirus (HPV) is known to be the cause of a range of malignancies. These entail benign infections like genital warts as well as malignant, life threatening conditions such as cervical cancer. Since a very high mortality rate is associated with HPV caused cancers (cervical cancer being 2nd leading cause of death caused due to cancer among women globally), there is an escalating need to understand and search for ways to combat such medical conditions. Under the same light, the given article provides an insight into the world of this versatile pathoge...
Source: Current Pharmaceutical Biotechnology - August 2, 2019 Category: Biotechnology Authors: Vashisht S, Mishra H, Mishra PK, Ekielski A, Talegaonkar S Tags: Curr Pharm Biotechnol Source Type: research

Recent Development in Fabrication of Collagen Scaffolds for Tissue Engineering Applications.
Abstract Tissue engineering focuses on developing biological substitutes to restore, maintain or improve tissue functions and the three main components of its application are scaffold, cell and growth-stimulating signals. The scaffolds made of biomaterials mainly function as the structural support for the ex vivo cells to attach and proliferate, and also provide physical, mechanical and biochemical cues for the cell differentiation before the cells are transferred to the in vivo site. Collagen have been long used in various clinical applications including as drug delivery vehicle. The wide usage of collagen in cli...
Source: Current Pharmaceutical Biotechnology - July 31, 2019 Category: Biotechnology Authors: Busra MFM, Lokanathan Y Tags: Curr Pharm Biotechnol Source Type: research

Glyoxal induced transition of transferrin to aggregates: Spectroscopic, microscopic and molecular docking insight.
Abstract The present study was aimed at characterizing the conformational alterations induced in human transferrin (hTF), the iron regulatory protein by glyoxal. Since, protein aggregation is at core of many disorders, thus interest in this domain has increased significantly during past years. In our present study, effect of glyoxal was monitored on hTF using multispectroscopic and multi-microscopic studies. Intrinsic fluorescence spectroscopy suggested transition of native hTF towards non-native form. Further, extrinsic fluorescence was retorted and the results were suggestive of this non-native form to be aggreg...
Source: Current Pharmaceutical Biotechnology - July 31, 2019 Category: Biotechnology Authors: Shamsi A, Abdullah KM, Usmani H, Shahab A, Hasan H, Naseem I Tags: Curr Pharm Biotechnol Source Type: research

A review on Targeting Nanoparticles for Breast Cancer.
Abstract Chemotherapeutic agents have been used extensively in breast cancer remedy. However, most anticancer drugs cannot differentiate between cancer cells and normal cells, leading to toxic side effects. Also, the resulted drug resistance during chemotherapy reduces treatment efficacy. The development of targeted drug delivery offers the great promise in breast cancer treatment both in clinical applications and in pharmaceutical research as well. This review discusses recent works in the targeting methods for breast cancer cells. In this review, we concentrate on chemical methods of targeting through the use of...
Source: Current Pharmaceutical Biotechnology - July 31, 2019 Category: Biotechnology Authors: Alqaraghuli HGJ, Kashanian S, Rafipour R Tags: Curr Pharm Biotechnol Source Type: research

In vitro anti-viral effects of small heat shock proteins 20 and 27: A novel therapeutic approach.
CONCLUSION: Generally, Hsp20 was identified as a novel anti-HCV, anti-HSV and anti-HIV agent, but Hsp27 was efficient in suppression of HSV infection. These Hsps may act through suppression of virus entry and/ or through interaction with viral proteins. Thus, it is necessary to determine their exact mechanisms in near future. PMID: 31362669 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 28, 2019 Category: Biotechnology Authors: Vahabpour R, Soleymani S, Roohvand F, Zabihollahi R, Bolhassani A Tags: Curr Pharm Biotechnol Source Type: research

Overexpression of miR-340-5p inhibits skin fibroblast proliferation by targeting Kruppel-like factor 2.
CONCLUSION: This study demonstrates that miR-340-5p may suppress skin fibroblast proliferation, and possibly through targeting KLF2. These findings could help in the understanding the function of miR-340-5p in skin fibroblast. And miR-340-5p could be a therapy target for preventing scarring. PMID: 31345144 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 25, 2019 Category: Biotechnology Authors: Chen L, Li Q, Lu X, Dong X, Li J Tags: Curr Pharm Biotechnol Source Type: research

Current advances on the extraction and identification of bioactive components of sage (Salvia spp.).
Abstract Common sage (Salvia spp., with the most common species Salvia officinalis L., Lamiaceae) is an important medicinal and aromatic plant, with antioxidant, antimicrobial, anti-inflammatory and anticancer properties. The polyphenolic compounds are mainly responsible for its diverse biological activity. Many different methods for extraction and identification of sage bioactive compounds, using various solvents, have been developed in recent years. The total phenolic content is usually measured and identified by various technical methodologies with different sensitivity and specificity. In this aspect, the pres...
Source: Current Pharmaceutical Biotechnology - July 22, 2019 Category: Biotechnology Authors: Poulios E, Giaginis C, Vasios GK Tags: Curr Pharm Biotechnol Source Type: research

Fabrication and in-vitro Investigation of PCL- (PVA/Collagen)Hybrid Nanofiber as Anti-Inflammatory GTR Membrane.
Conclusion- The membranes indicated proper physicochemical properties and appropriate biodegradation rate as a potential GTR membrane. PMID: 31333124 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 22, 2019 Category: Biotechnology Authors: Limoee M, Moradipour P, Arkan E, Behbood L Tags: Curr Pharm Biotechnol Source Type: research

Tinospora cordifolia aqueous extract alleviates cyclophosphamide-induced immune suppression, toxicity and systemic candidiasis in immunosuppressed mice: In vivo study in comparison to antifungal drug fluconazole.
CONCLUSIONS: These results suggest that AETC may potentially be used to rejuvenate the weakened immune system and eliminate the systemic candidiasis in mice. PMID: 31333126 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 22, 2019 Category: Biotechnology Authors: Alrumaihi F, Allemailem KS, Almatroudi A, Alsahli MA, Khan A, Khan MA Tags: Curr Pharm Biotechnol Source Type: research

Identification of a Potential Inhibitor Targeting MurC Ligase of the Drug Resistant Pseudomonas aeruginosa Strain through Structure-Based Virtual Screening Approach and In Vitro Assay.
In this study, a homology model of Pseudomonas aeruginosa MurC ligase was generated and used for virtual screening of chemical compounds from the Zinc Database. The best screened inhibitor i.e. N,N-dimethyl-2-oxo-2,3-dihydro-1H-1,3-benzodiazole-5-sulfonamide was then validated experimentally through inhibition assay. The presented results based on combined computational and in vitro analysis open up new horizons for the development of novel antimicrobials against this pathogen. PMID: 31333120 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 19, 2019 Category: Biotechnology Authors: Messaoudi A, Zoghlami M, Basharat Z, Sadfi-Zouaoui N Tags: Curr Pharm Biotechnol Source Type: research

Evaluation of the synergistic antioxidant effect of resveratrol and Zataria multiflora Boiss essential oil in sodium alginate bioactive films.
Abstract Biodegradable films are the appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as carrier for wide range of food additives especially natural antioxidants. The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model. At first, the major chemical compositions of ZEO were evaluated by GC/MS. Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic co...
Source: Current Pharmaceutical Biotechnology - July 19, 2019 Category: Biotechnology Authors: Hashemi M, Hashemi M, Amiri E, Hassanzadazar H, Daneshamooz S, Aminzare M Tags: Curr Pharm Biotechnol Source Type: research

Expression profile of VEGF-C, VEGF-D, VEGFR-3 in different grades of endometrial cancer.
CONCLUSION: An increase in VEGF-D and VEGFR-3 levels may indicate that VEGF-D dependent processes are intensified along with the dedifferentiation of tumor cells. The lack of VEGF-C expression in endometrial cancer samples may suggest that this tumor is characterized by a mechanism of metastasis other than EMT. Our study emphasize that when analyzing the metastatic potential of cancer, the expression of more than one factor should be taken into account. PMID: 31333122 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 18, 2019 Category: Biotechnology Authors: Oplawski M, Dziobek K, Zmarzły N, Grabarek B, Halski T, Januszyk P, Kuś-Kierach A, Adwent IA, Dąbruś D, Kiełbasiński K, Boron D Tags: Curr Pharm Biotechnol Source Type: research

Biotechnological production of statins: metabolic aspects and genetic approaches.
da RC Abstract Statins are a class of drugs used for people with abnormal lipid levels (hyperlipidemia) that are among the best-selling medications in the United States. Thus, the biotechnological production of these drugs is of extreme importance for the pharmaceutical industry. Herein, we provide a non-exhaustive review of fungal species used to produce statin and highlighted the major factors affecting the efficacy of this process. The current biotechnological approaches and the advances of metabolic engineer to improve statins production by fungi are also emphasised. The biotechnological production of the main...
Source: Current Pharmaceutical Biotechnology - July 18, 2019 Category: Biotechnology Authors: Moraes Neto RN, Barros Gomes E, Weba-Soares L, Dias LR, Nascimento da Silva LC, Mendonça de Miranda RC Tags: Curr Pharm Biotechnol Source Type: research

Plant Phenolics and Lectins as Vaccine Adjuvants.
CONCLUSION: Vaccine adjuvants are chemical substances of very varied structure that seek to improve the immunogenicity of antigens. The main four types of adjuvants under investigation are the following: 1) Oil emulsions with an antigen in solution, 2) Pattern recognition receptors activating molecules, 3) Inflammatory stimulatory molecules or activators of the inflammasome complex, and 4) Cytokines. However, this paper addresses the biological plausibility of two phytochemical compounds as vaccine adjuvants: 5) Lectins, and 6) Plant phenolics whose characteristics, mechanisms of action and disadvantages are addressed. Fin...
Source: Current Pharmaceutical Biotechnology - July 16, 2019 Category: Biotechnology Authors: Reyna-Margarita HR, Irais CM, Mario-Alberto RG, Agustina RM, Luis-Benjamín SG, David PE Tags: Curr Pharm Biotechnol Source Type: research

Evaluation of the Glucuronic Acid Production and Antibacterial Properties of Kombucha Black Tea.
CONCLUSIONS: This study offers a perspective on glucuronic acid production (especially in 30°C rather than 20°C) and antibacterial activity of Kombucha black tea beverage. PMID: 31322066 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 16, 2019 Category: Biotechnology Authors: Ansari F, Pourjafar H, Kangari A, Homayouni A Tags: Curr Pharm Biotechnol Source Type: research

Systematic review of economic evaluation studies of drug-based non-malignant chronic pain treatment.
CONCLUSION: There are limitations to the success of making appropriate recommendations about which treatment is most cost-effective due to great variability between treatments, pain syndromes, and drug dosages. To ensure correct resource allocation as well the best benefit for patients, uniform and standardized approaches of cost and outcome measurement in economic evaluations of chronic are needed. PMID: 31322067 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 16, 2019 Category: Biotechnology Authors: Backhaus I, Mannocci A, La Torre G Tags: Curr Pharm Biotechnol Source Type: research

Changes in the Expression Profile of VEGF-A, VEGF-B, VEGFR-1, VEGFR-2 in Different Grades of Endometrial Cancer.
CONCLUSION: VEGF-A, VEGF-B, VEGF-R1, VEGFR-2 are key proteins involved in tumor angiogenesis. The analysis of the entire panel of proteins involved in a given process is an important element of modern diagnostics. The concentration ratio of VEGFR-1 to VEGFR-2 appears to be a determining factor in the patients' survival prognosis. PMID: 31322068 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 16, 2019 Category: Biotechnology Authors: Dziobek K, Opławski M, Grabarek B, Zmarzły N, Tomala B, Halski T, Leśniak E, Januszyk K, Brus R, Kiełbasiński R, Boroń D Tags: Curr Pharm Biotechnol Source Type: research

Nanoparticles and zeolites: Antibacterial effects and their mechanism against pathogens.
Abstract Nowadays, distribution and microorganism resistance against antimicrobial compounds have caused crucial food safety problems. Hence, nanotechnology and zeolite are recognized as new approaches to manage this problem due to their inherent antimicrobial activity. Different studies have confirmed antimicrobial effects of Nano particles (NPs) (metal and metal oxide) and zeolite, by using various techniques to determine antimicrobial mechanism. This review includes an overview of research with the results of studies about antimicrobial mechanisms of nanoparticles and zeolite. Many researches have shown that ty...
Source: Current Pharmaceutical Biotechnology - July 8, 2019 Category: Biotechnology Authors: Azizi-Lalabadi M, Ehsani A, Alizadeh-Sani M, Khezerlou A, Mirzanajafi-Zanjani M, Divband B, Zolfaghari H, Bagheri V Tags: Curr Pharm Biotechnol Source Type: research

Cardiotoxicity assessment of drugs using human iPS cell-derived cardiomyocytes: From proarrhythmia risk to cardiooncology.
Abstract Growing evidence suggests that human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) can be used as a new human cell-based platform to assess cardiac toxicity/safety during drug development. Cardiotoxicity assessment is highly challenging due to species differences and various toxicities, such as electrophysiological and contractile toxicities, which can result in proarrhythmia and heart failure. To explore proarrhythmia risk, the multi-electrode array (MEA) platform is widely used to assess QT-interval prolongation and the proarrhythmic potential of drug candidates using hiPSC-CMs. Sever...
Source: Current Pharmaceutical Biotechnology - June 28, 2019 Category: Biotechnology Authors: Satsuka A, Kanda Y Tags: Curr Pharm Biotechnol Source Type: research