Integrated micro ‐scale protein a chromatography and Low pH viral inactivation unit operations on an automated platform
This study is based upon the design and testing of a HTPD platform for integration of the protein A and low pH VI operations. This was achieved by using a design and simulation software before execution on an automated liquid handler. The operations were successfully translated to the micro-scale, as assessed by analysis of recoveries and molecular weight content. The integrated platform was then used as a tool to assess the effect of pH on HMWC during low pH hold. The laboratory-scale and micro-scale elution pools showed comparable HMWC across the pH range 3.2 –3.7. The investigative power of the platform is highlighted...
Source: Biotechnology Progress - May 1, 2024 Category: Biotechnology Authors: Paras Sharma,
Lars Robbel,
Michael Schmitt,
Duygu Dikicioglu,
Daniel G. Bracewell Tags: RESEARCH ARTICLE Source Type: research
Surface plasmon resonance aptasensing and computational analysis of Staphylococcus aureus IsdA surface protein
AbstractStaphylococcus aureus (S. aureus), a common foodborne pathogen, poses significant public health challenges due to its association with various infectious diseases. A key player in its pathogenicity, which is the IsdA protein, is an essential virulence factor inS. aureus infections. In this work, we present an integrated in-silico and experimental approach using MD simulations and surface plasmon resonance (SPR)-based aptasensing measurements to investigateS. aureus biorecognition via IsdA surface protein binding. SPR, a powerful real-time and label-free technique, was utilized to characterize interaction dynamics b...
Source: Biotechnology Progress - April 30, 2024 Category: Biotechnology Authors: Tracy Ann Bruce ‐Tagoe,
Michael T. Harnish,
Shokoufeh Soleimani,
Najeeb Ullah,
Tongye Shen,
Michael K. Danquah Tags: RESEARCH ARTICLE Source Type: research
Multivariate data analysis of process parameters affecting the growth and productivity of stable Chinese hamster ovary cell pools expressing SARS ‐CoV‐2 spike protein as vaccine antigen in early process development
In this study, we analyze early process development data of recombinant trimeric spike protein Cumate-inducible manufacturing platform utilizing CHO stable pool as a preferred production host across three different stirred-tank bioreactor scales (0.75, 1, and 10 L). The impact of cell passage number as an indicator of cell age, methionine sulfoximine (MSX) concentration as a selection pressure, and cell seeding density was investigated using stable pools expressing three variants of concern. Multivariate data analysis with principal component analysis an d batch-wise unfolding technique was applied to evaluate the effec...
Source: Biotechnology Progress - April 26, 2024 Category: Biotechnology Authors: Sebastian ‐Juan Reyes,
Lucas Lemire,
Raul‐Santiago Molina,
Marjolaine Roy,
Helene L'Ecuyer‐Coelho,
Yuliya Martynova,
Brian Cass,
Robert Voyer,
Yves Durocher,
Olivier Henry,
Phuong Lan Pham Tags: RESEARCH ARTICLE Source Type: research
Improved sieving coefficient in perfusion cell culture with reduced effective filtration length of hollow fibers
AbstractThe hollow fiber filter is the primary cell-retention device used in high-density perfusion cell culture and often used in an alternating tangential flow (ATF) configuration. The limited commercially available diaphragm pumps for ATF prevent utilization of vertical space when scaling beyond 500 L. Stacking hollow fiber filters coupled with viscous cell culture imposes vacuum pressure exceeding facility capabilities. Additionally, the longer filter assembly increases the hold-up volume and exceeds the diaphragm pump's fluid exchange capacity. The conventional tangential flow filtration ( TFF) configuration circum...
Source: Biotechnology Progress - April 25, 2024 Category: Biotechnology Authors: Jimmy Vu,
J. Alex Gadberry,
Jon Coffman,
Ken Lee Tags: RESEARCH ARTICLE Source Type: research
Pharmaceutical proteins at the interfaces and the role of albumin
AbstractA critical measure of the quality of pharmaceutical proteins is the preservation of native conformations of the active pharmaceutical ingredients. Denaturation of the active proteins in any step before administration into patients could lead to loss of potency and/or aggregation, which is associated with an increased risk of immunogenicity of the products. Interfacial stress enhances protein instability as their adsorption to the air-liquid and liquid –solid interfaces are implicated in the formation of denatured proteins and aggregates. While excipients in protein formulations have been employed to reduce the ri...
Source: Biotechnology Progress - April 23, 2024 Category: Biotechnology Authors: Ketki Y. Velankar,
Ellen S. Gawalt,
Yi Wen,
Wilson S. Meng Tags: REVIEW Source Type: research
Effects of green light supplementation with red and blue combinations of LED light spectrums on the growth and transcriptional response of Haematococcus pluvialis
In this study, the effects of green light supplementation, in the combination with red and blue light were investigated inHaematococcus pluvialis. 10% and 20% of green light supplementations were applied in 3:2 ratios of red and blue LED light combinations as an expense of red-light. Growth rates, chlorophyll concentration, and dry weight were measured to assess the growth kinetics ofH. pluvialis along with the relative transcript accumulations of four mRNAs: Rubisco, PTOX2, PsaB, and PsbS. Growth rates, chlorophyll concentrations and dry weight were found significantly higher in presence of 10% green light supplementation...
Source: Biotechnology Progress - April 21, 2024 Category: Biotechnology Authors: G. Karag ülle,
M. Telli Tags: RESEARCH ARTICLE Source Type: research
Issue Information
(Source: Biotechnology Progress)
Source: Biotechnology Progress - April 18, 2024 Category: Biotechnology Tags: ISSUE INFORMATION Source Type: research
Strategies to improve CHO cell culture performance: Targeted deletion of amino acid catabolism and apoptosis genes paired with growth inhibitor supplementation
AbstractChinese hamster ovary (CHO) cells are the predominant host of choice for recombinant monoclonal antibody (mAb) expression. Recent advancements in gene editing technology have enabled engineering new CHO hosts with higher growth, viability, or productivity. One approach involved knock out (KO) of BCAT1 gene, which codes for the first enzyme in the branched chain amino acid (BCAA) catabolism pathway; BCAT1 KO reduced accumulation of growth inhibitory short chain fatty acid (SCFA) byproducts and improved culture growth and titer when used in conjunction with high-end pH-controlled delivery of glucose (HiPDOG) technolo...
Source: Biotechnology Progress - April 18, 2024 Category: Biotechnology Authors: Cynthia Lam,
Alyssa Sargon,
Camil Diaz,
Zijuan Lai,
Dewakar Sangaraju,
Inn Yuk,
Gavin Barnard,
Shahram Misaghi Tags: RESEARCH ARTICLE Source Type: research
Single cell analysis of Chinese hamster ovary cells during a bioprocess using a novel dynamic imaging system
This article assesses a novel dynamic imaging system for single cell analysis. This data shows that comparable total cell density, viable cell density and percentage viability data shown here, generated by the imaging system, aligned well with conventional trypan blue counting methods for an industrially relevant Chinese Hamster Ovary (CHO) cell line. Furthermore, detailed statistical analysis shows that the classification system used by the PharmaFlow system can reveal trends of interest in monitoring the health of mammalian cells over a 6-day bioreactor culture. The system is also capable of sampling at-line, removing th...
Source: Biotechnology Progress - April 14, 2024 Category: Biotechnology Authors: Laura Breen,
James Flynn,
Adam Bergin,
Evangelia Flampouri,
Michael Butler Tags: RESEARCH ARTICLE Source Type: research
Monte Carlo simulation ‐guided design for size‐tuned tumor spheroid formation in 3D printed microwells
This study showed prominent steps in investigating spheroid formation, thereby leveraging the current know-how on the mechanism of tumor growth. (Source: Biotechnology Progress)
Source: Biotechnology Progress - April 13, 2024 Category: Biotechnology Authors: Ismail E ş,
Ana‐Maria Theodora Ionescu,
Burak M. Görmüş,
Fatih Inci,
Marco P. C. Marques,
Nicolas Szita,
Lucimara Gaziola de la Torre Tags: RESEARCH ARTICLE Source Type: research
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cell expression system
AbstractMonoclonal antibodies (mAbs) are often engineered at the sequence level for improved clinical performance yet are rarely evaluated prior to candidate selection for their “developability” characteristics, namely expression, which can necessitate additional resource investments to improve the manufacturing processes for problematic mAbs. A strong relationship between primary sequence and expression has emerged, with slight differences in amino acid sequence result ing in titers differing by up to an order of magnitude. Previous work on these “difficult-to-express” (DTE) mAbs has shown that these phenotypes ar...
Source: Biotechnology Progress - April 13, 2024 Category: Biotechnology Authors: Alana C. Szkodny,
Kelvin H. Lee Tags: RESEARCH ARTICLE Source Type: research
Online monitoring of the respiration activity in 96 ‐deep‐well microtiter plate Chinese hamster ovary cultures streamlines kill curve experiments
In conclusion, the broad application of OTR online monitoring for CHO cell cultures in 96-deep-well plates is highlighted. The μTOM device proves as a valuable tool for high-throughput experiments, paving the way fo r diverse applications, such as media and clone screening, cytotoxicity tests, and scale-up experiments. (Source: Biotechnology Progress)
Source: Biotechnology Progress - April 12, 2024 Category: Biotechnology Authors: Anne Neuss,
Nele von Vegesack,
Raoul Liepelt,
Jochen B üchs,
Jørgen Barsett Magnus Tags: RESEARCH ARTICLE Source Type: research
Mesenchymal stem cells derived ‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation
In this study, we evaluated the effects of both AME and WJ-MSC-Exos, individually and together, on the proliferation of corneal keratocytes as well as their ability to promote in vitro cell migration, wound healing, and their impact on cellular morphology. Our findings indicated that the presence of both exosomes (3 × 105 Exo/mL) and AME (50 μg/mL) synergistically enhance the proliferation of corneal keratocytes. Combined use of these solutions (3 × 105 Exo/mL + 50 μg/mL) increased cell proliferation compared to only 50 μg/mL AME treatment on day 3 (****p < 0.0001). This mixture treatmen...
Source: Biotechnology Progress - April 11, 2024 Category: Biotechnology Authors: Fatma Zehra Erkoc ‐Biradli,
Berkay Erenay,
Alp Ozgun,
Hayriye Öztatlı,
Ferda Işık,
Utku Ateş,
Rıfat Rasier,
Bora Garipcan Tags: RESEARCH ARTICLE Source Type: research
Development of a pan ‐tau multivalent nanobody that binds tau aggregation motifs and recognizes pathological tau aggregates
AbstractAlzheimer's disease and other tauopathies are characterized by the misfolding and aggregation of the tau protein into oligomeric and fibrillar structures. Antibodies against tau play an increasingly important role in studying these neurodegenerative diseases and the generation of tools to diagnose and treat them. The development of antibodies that recognize tau protein aggregates, however, is hindered by complex immunization and antibody selection strategies and limitations to antigen presentation. Here, we have taken a facile approach to identify single-domain antibodies, or nanobodies, that bind to many forms of ...
Source: Biotechnology Progress - April 4, 2024 Category: Biotechnology Authors: Nikki McArthur,
Bokyung Kang,
Felix G. Rivera Moctezuma,
Akber T. Shaikh,
Kathryn Loeffler,
Nemil N. Bhatt,
Madison Kidd,
Jennifer M. Zupancic,
Alec A. Desai,
Naima Djeddar,
Anton Bryksin,
Peter M. Tessier,
Rakez Kayed,
Levi B. Wood,
Ravi S. Tags: RESEARCH ARTICLE Source Type: research
CD133 ligand ‐enhanced etoposide‐liposome complex for targeted killing of lung cancer cells
In this study, we combined nanotechnology and biotechnology to develop a CD133 ligand-modified etoposide-liposome complex (Lipo@ETP-CD133) for targeted therapy of lung cancer. The CD133 ligand targeted lung cancer stem cells, causing the composite material to aggregate at the tumor site, where high levels of ETP liposomes could exert a strong tumor-killing effect. Our research results demonstrated that this nano-drug had efficient targeting and tumor-killing effects, indicating its potential for clinical application. (Source: Biotechnology Progress)
Source: Biotechnology Progress - April 2, 2024 Category: Biotechnology Authors: Shiwei Nie,
Junzheng Zhou,
Xiaodong Zheng,
Xudong Wei,
Jinrui Zhang,
Xiaojuan Shen,
Weimin Zhang Tags: RESEARCH ARTICLE Source Type: research
