Clearance of porcine circovirus and porcine parvovirus from porcine ‐derived pepsin by low pH inactivation and cation exchange chromatography
In this study, we investigated low pH inactivation of viruses including PCV Type 1 (PCV1) and PCV1 removal by cation exchange chromatography (CEX) in the presence of pepsin. Both parvovirus and PCV1 could be effectively inactivated by low pH and PCV1 could be removed by POROS 50HS CEX. The POROS 50HS method presented in this article is helpful for designing other CEX methods for the same purpose and not much difference would be expected for similar product intermediates and same process parameters. While the effectiveness needs to be confirmed for specific applications, the results demonstrate that both low pH (pH 1...
Source: Biotechnology Progress - February 14, 2020 Category: Biotechnology Authors: Bin Yang, Hua Wang, Kimberly Kaleas, Michelle Butler, Jayme Franklin, Amber Bill, Sally A. Baylis, Qi Chen, Johannes Bl ümel Tags: Research Article Source Type: research

Dewatering of algal suspension using microfiltration with cross flow in the presence of magnetite as a filter aid
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - February 12, 2020 Category: Biotechnology Authors: Hidemi Kitani, Mikihide Demura, Shintaro Morisada, Keisuke Ohto, Hidetaka Kawakita Tags: RESEARCH ARTICLE Source Type: research

Issue Information
Biotechnology Progress, Volume 36, Issue 1, January/February 2020. (Source: Biotechnology Progress)
Source: Biotechnology Progress - February 11, 2020 Category: Biotechnology Tags: ISSUE INFORMATION Source Type: research

Multiplexed clonality verification of cell lines for protein biologic production
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - February 8, 2020 Category: Biotechnology Authors: Sofie A. O'Brien, Juhi Ojha, Paul Wu, Wei ‐Shou Hu Tags: RESEARCH ARTICLE Source Type: research

Manufacturing biological medicines on demand: Safety and efficacy of granulocyte colony ‐stimulating factor in a mouse model of total body irradiation
The objective of the current study is to establish proof‐of‐concept safety an d efficacy of Bio‐MOD‐manufactured granulocyte colony‐stimulating factor (G‐CSF) in a mouse model of total body irradiation at a dose estimated to induce 30% lethality within the first 30 days postexposure. To illustrate on‐demand Bio‐MOD production feasibility, histidine‐tagged G‐ CSF was manufactured daily under good manufacturing practice‐like conditions prior to administration over a 16‐day period. Bio‐MOD‐manufactured G‐CSF improved 30‐day survival when compared with saline alone (p = .073). In addition...
Source: Biotechnology Progress - February 7, 2020 Category: Biotechnology Authors: Rajani Adiga, Abhay Andar, Shayan Borhani, David Burgenson, Sevda Deldari, Douglas Frey, Xudong Ge, Chandrasekhar Gurramkonda, Erick Gutierrez, Isabel L. Jackson, Yordan Kostov, Yang Liu, Diana Newman, Joseph Piegols, Benjamin Punshon ‐Smi Tags: RESEARCH ARTICLE Source Type: research

Characterization of three ‐dimensional rat central nervous system culture maturation, with applications to monitor cholinergic integrity
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - February 5, 2020 Category: Biotechnology Authors: Parker Lyng Andersen, Patrick Vermette, Abdelouahed Khalil, Jacek M. Witkowski, Tamas F ülöp Tags: RESEARCH ARTICLE Source Type: research

Feeding tricarboxylic acid (TCA) cycle intermediates improves lactate consumption and antibody production in CHO cell cultures
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - February 5, 2020 Category: Biotechnology Authors: Xiaolin Zhang, Rubin Jiang, Henry Lin, Sen Xu Tags: RESEARCH ARTICLE Source Type: research

Analysis of chemometric models applied to Raman spectroscopy for monitoring key metabolites of cell culture
This study compares the chemometric modeling methods of Support Vector Machine radial (SVMr), Random Forests (RF) and Cubist to the commonly used linear PLS model for predicting cell culture components – glucose, lactate and ammonia. This research is performed to assess if the use of PLS as standard practice is justified for chemometric modeling of Raman spectroscopy and cell culture data. Model development data from 5 small scale bioreactors (2 x 1 L& 3 x 5  L) using two Chinese Hamster Ovary (CHO) cell lines were used to predict against a manufacturing scale bioreactor (2000 L). Analysis de...
Source: Biotechnology Progress - February 5, 2020 Category: Biotechnology Authors: Carl Rafferty, Kjell Johnson, Jim O'Mahony, Barbara Burgoyne, Rosemary Rea, Karin M. Balss Tags: RESEARCH ARTICLE Source Type: research

Characterization of three ‐dimensional rat central nervous system culture maturation, with applications to monitor cholinergic integrity
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - February 4, 2020 Category: Biotechnology Authors: Parker Lyng Andersen, Patrick Vermette, Abdelouahed Khalil, Jacek M. Witkowski, Tamas F ülöp Tags: RESEARCH ARTICLE Source Type: research

Feeding tricarboxylic acid (TCA) cycle intermediates improves lactate consumption and antibody production in CHO cell cultures
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - February 4, 2020 Category: Biotechnology Authors: Xiaolin Zhang, Rubin Jiang, Henry Lin, Sen Xu Tags: RESEARCH ARTICLE Source Type: research

Analysis of chemometric models applied to Raman spectroscopy for monitoring key metabolites of cell culture
This study compares the chemometric modeling methods of Support Vector Machine radial (SVMr), Random Forests (RF) and Cubist to the commonly used linear PLS model for predicting cell culture components – glucose, lactate and ammonia. This research is performed to assess if the use of PLS as standard practice is justified for chemometric modeling of Raman spectroscopy and cell culture data. Model development data from 5 small scale bioreactors (2 x 1 L& 3 x 5  L) using two Chinese Hamster Ovary (CHO) cell lines were used to predict against a manufacturing scale bioreactor (2000 L). Analysis de...
Source: Biotechnology Progress - February 4, 2020 Category: Biotechnology Authors: Carl Rafferty, Kjell Johnson, Jim O'Mahony, Barbara Burgoyne, Rosemary Rea, Karin M. Balss Tags: RESEARCH ARTICLE Source Type: research

Clearance of Porcine Circovirus (PCV) and Porcine Parvovirus (PPV) from Porcine ‐Derived Pepsin by Low pH Inactivation and Cation Exchange Chromatography
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Bin Yang, Hua Wang, Kimberly Kaleas, Michelle Butler, Jayme Franklin, Amber Bill, Sally A. Baylis, Qi Chen, Johannes Bl ümel Tags: Research Article Source Type: research

Designing a new dimerized Anti ‐human TNF‐α aptamer with blocking activity
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Kazem Mashayekhi, Ali Ganji, Mojtaba Sankian Tags: RESEARCH ARTICLE Source Type: research

Manufacturing Biological Medicines on Demand: Safety and Efficacy of Granulocyte ‐Colony Stimulating Factor in a Mouse Model of Total Body Irradiation
The objective of the current study is to establish proof‐of‐concept safety and efficacy of Bio‐MOD‐manufactured granulocyte colony stimulating factor (G‐CSF) in a mouse model of total body irradiation at a dose estimated to induce 30% lethality withi n the first 30‐days post‐exposure. To illustrate on‐demand Bio‐MOD production feasibility, histidine tagged G‐CSF was manufactured daily under good manufacturing practice (GMP)‐like conditions prior to administration over a 16‐day period. Bio‐MOD‐manufactured G‐CSF improved 30‐ day survival when compared to saline alone (p = 0.073). In...
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Rajani Adiga, Abhay Andar, Shayan Borhani, David Burgenson, Sevda Deldari, Douglas Frey, Xudong Ge, Chandrasekhar Gurramkonda, Erick Gutierrez, Isabel L. Jackson, Yordan Kostov, Yang Liu, Diana Newman, Joseph Piegols, Benjamin Punshon ‐Smi Tags: RESEARCH ARTICLE Source Type: research

Inhibition of endogenous miR ‐23a/miR‐377 in CHO cells enhances difficult‐to‐express recombinant lysosomal sulfatase activity
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Ifeanyi Michael Amadi, Vishal Agrawal, Terri Christianson, Cameron Bardliving, Parviz Shamlou, Jonathan H. LeBowitz Tags: RESEARCH ARTICLE Source Type: research

Improving Product Quality and Productivity of Bispecific Molecules Through the Application of Continuous Perfusion Principles
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Natalia Gomez, Jonathan Lull, Xiaorui Yang, Yan Wang, Xin Zhang, Agatha Wieczorek, John Harrahy, Mike Pritchard, Diandra Martinez Cano, Michael Shearer, Chetan Goudar Tags: RESEARCH ARTICLE Source Type: research

Biocalorimetric monitoring of glycoengineered P. pastoris cultivation for the production of recombinant huIFN α2b: A quantitative study based on mixed feeding strategies
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Srikanth Katla, Satya Sai Pavan Allampalli, Naresh Mohan, Senthilkumar Sivaprakasam Tags: Research Article Source Type: research

Exploiting the NADPH pool for xylitol production using recombinant Saccharomyces cerevisiae
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Shamlan M. S. Reshamwala, Arvind M. Lali Tags: Research Article Source Type: research

Clearance of Porcine Circovirus (PCV) and Porcine Parvovirus (PPV) from Porcine ‐Derived Pepsin by Low pH Inactivation and Cation Exchange Chromatography
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Bin Yang, Hua Wang, Kimberly Kaleas, Michelle Butler, Jayme Franklin, Amber Bill, Sally A. Baylis, Qi Chen, Johannes Bl ümel Tags: Research Article Source Type: research

Designing a new dimerized Anti ‐human TNF‐α aptamer with blocking activity
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Kazem Mashayekhi, Ali Ganji, Mojtaba Sankian Tags: RESEARCH ARTICLE Source Type: research

Manufacturing Biological Medicines on Demand: Safety and Efficacy of Granulocyte ‐Colony Stimulating Factor in a Mouse Model of Total Body Irradiation
The objective of the current study is to establish proof‐of‐concept safety and efficacy of Bio‐MOD‐manufactured granulocyte colony stimulating factor (G‐CSF) in a mouse model of total body irradiation at a dose estimated to induce 30% lethality withi n the first 30‐days post‐exposure. To illustrate on‐demand Bio‐MOD production feasibility, histidine tagged G‐CSF was manufactured daily under good manufacturing practice (GMP)‐like conditions prior to administration over a 16‐day period. Bio‐MOD‐manufactured G‐CSF improved 30‐ day survival when compared to saline alone (p = 0.073). In...
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Rajani Adiga, Abhay Andar, Shayan Borhani, David Burgenson, Sevda Deldari, Douglas Frey, Xudong Ge, Chandrasekhar Gurramkonda, Erick Gutierrez, Isabel L. Jackson, Yordan Kostov, Yang Liu, Diana Newman, Joseph Piegols, Benjamin Punshon ‐Smi Tags: RESEARCH ARTICLE Source Type: research

Inhibition of endogenous miR ‐23a/miR‐377 in CHO cells enhances difficult‐to‐express recombinant lysosomal sulfatase activity
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Ifeanyi Michael Amadi, Vishal Agrawal, Terri Christianson, Cameron Bardliving, Parviz Shamlou, Jonathan H. LeBowitz Tags: RESEARCH ARTICLE Source Type: research

Improving Product Quality and Productivity of Bispecific Molecules Through the Application of Continuous Perfusion Principles
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Natalia Gomez, Jonathan Lull, Xiaorui Yang, Yan Wang, Xin Zhang, Agatha Wieczorek, John Harrahy, Mike Pritchard, Diandra Martinez Cano, Michael Shearer, Chetan Goudar Tags: RESEARCH ARTICLE Source Type: research

Biocalorimetric monitoring of glycoengineered P. pastoris cultivation for the production of recombinant huIFN α2b: A quantitative study based on mixed feeding strategies
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Srikanth Katla, Satya Sai Pavan Allampalli, Naresh Mohan, Senthilkumar Sivaprakasam Tags: Research Article Source Type: research

Exploiting the NADPH pool for xylitol production using recombinant Saccharomyces cerevisiae
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 28, 2020 Category: Biotechnology Authors: Shamlan M. S. Reshamwala, Arvind M. Lali Tags: Research Article Source Type: research

Next ‐generation polymerized human hemoglobins in hepatic bioreactor simulations
In this study, T ‐state PolyhHb production and purification processes were optimized to minimize the concentration of low‐molecular‐weight PolyhHb species in solution. Deconvolution of size‐exclusion chromatography spectra was performed to calculate the distribution of polymeric Hb species in the final produ ct. Fluid flow and mass transport within a single fiber of a hepatic HF bioreactor was computationally modeled with finite element methods to simulate the effects of employing T‐state PolyhHb to facilitate O2 transport in a hepatic bioreactor system. Optimal bioreactor performance was defined as having a comb...
Source: Biotechnology Progress - January 21, 2020 Category: Biotechnology Authors: Clayton Cuddington, Savannah Moses, Donald Belcher, Niral Ramesh, Andre Palmer Tags: RESEARCH ARTICLE Source Type: research

Superhydrophobic nanocarbon ‐based membrane with antibacterial characteristics
AbstractTo overcome the biofouling challenge which faces membrane water treatment processed, the novel superhydrophobic carbon nanomaterials impregnated on/powder activated carbon (CNMs/PAC) was utilized to successfully design prepare an antimicrobial membrane. The research was conducted following a systematic statistical design of experiments technique considering various parameters of composite membrane fabrication. The impact of these parameters of composite membrane onStaphylococcus aureus growth was investigated. The bacteria growth was analyzed through spectrophotometer and SEM. The effect of CNMs' hydrophobicity on ...
Source: Biotechnology Progress - January 21, 2020 Category: Biotechnology Authors: Mustafa M. Aljumaily, Mohammed A. Alsaadi, Nur Awanis Binti Hashim, Farouq S. Mjalli, Qusay F. Alsalhy, Abdul L. Khan, Ahmed Al ‐Harrasi Tags: RESEARCH ARTICLE Source Type: research

Maximizing Antibody Production in a Targeted Integration Host by Optimization of Subunit Gene Dosage and Position
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 21, 2020 Category: Biotechnology Authors: Joe Carver, Domingos Ng, Michelle Zhou, Peggy Ko, Dejin Zhan, Mandy Yim, David Shaw, Brad Snedecor, Michael W. Laird, Steven Lang, Amy Shen, Zhilan Hu Tags: RESEARCH ARTICLE Source Type: research

Next ‐generation polymerized human hemoglobins in hepatic bioreactor simulations
In this study, T ‐state PolyhHb production and purification processes were optimized to minimize the concentration of low‐molecular‐weight PolyhHb species in solution. Deconvolution of size‐exclusion chromatography spectra was performed to calculate the distribution of polymeric Hb species in the final produ ct. Fluid flow and mass transport within a single fiber of a hepatic HF bioreactor was computationally modeled with finite element methods to simulate the effects of employing T‐state PolyhHb to facilitate O2 transport in a hepatic bioreactor system. Optimal bioreactor performance was defined as having a comb...
Source: Biotechnology Progress - January 21, 2020 Category: Biotechnology Authors: Clayton Cuddington, Savannah Moses, Donald Belcher, Niral Ramesh, Andre Palmer Tags: RESEARCH ARTICLE Source Type: research

Generation of keratinocyte stem ‐like cells from human fibroblasts via a direct reprogramming approach
AbstractSkin repair and reconstruction are important after severe wound and trauma. Keratinocyte stem cells (KSCs) in the basal layer of the epidermis can regrow the stratified epidermis but are almost depleted after skin injury. Thus, generating enough KSCs is indispensable for skin regeneration. Pluripotent stem cells such as ESC and iPSC can differentiate into KSCs, but their applications are challenged by ethical issues and risks of tumor formation. Lineage reprogramming from one cell type into another one makes it feasible to generate the desired cell type. Here, we develop a method to convert human fibroblasts into i...
Source: Biotechnology Progress - January 21, 2020 Category: Biotechnology Authors: Andong Zhao, Yi Yang, Xiaohua Pan, Yu Pan, Sa Cai Tags: RESEARCH ARTICLE Source Type: research

Superhydrophobic nanocarbon ‐based membrane with antibacterial characteristics
AbstractTo overcome the biofouling challenge which faces membrane water treatment processed, the novel superhydrophobic carbon nanomaterials impregnated on/powder activated carbon (CNMs/PAC) was utilized to successfully design prepare an antimicrobial membrane. The research was conducted following a systematic statistical design of experiments technique considering various parameters of composite membrane fabrication. The impact of these parameters of composite membrane onStaphylococcus aureus growth was investigated. The bacteria growth was analyzed through spectrophotometer and SEM. The effect of CNMs' hydrophobicity on ...
Source: Biotechnology Progress - January 21, 2020 Category: Biotechnology Authors: Mustafa M. Aljumaily, Mohammed A. Alsaadi, Nur Awanis Binti Hashim, Farouq S. Mjalli, Qusay F. Alsalhy, Abdul L. Khan, Ahmed Al ‐Harrasi Tags: RESEARCH ARTICLE Source Type: research

Optimized immobilization of endoglucanase Cel9A onto glutaraldehyde activated chitosan nanoparticles by response surface methodology: The study of kinetic behaviors
In this study, chitosan nanoparticles (ChNPs) were prepared by Ionic gelation method and Endoglucanase Cel9A fromAlicyclobacillus acidocaldariius (AaCel9A) immobilized on the nanoparticles. The FTIR results showed that the enzymes were immobilized on the ChNPs. The dynamic light scattering and scanning electron microscope (SEM) results illustrated that the AaCel9A ‐ChNPs approximately had 40 nm diameters. For optimizing enzyme immobilization, response surface methodology was employed using different variables (pH, enzyme immobilization time, and enzyme to ChNPs ratio [E/Cs]). The results showed that the high immob...
Source: Biotechnology Progress - January 21, 2020 Category: Biotechnology Authors: Saeed Najavand, Mahdiyeh Habibnejad, A. R. Amani ‐Ghadim, Parastou Rahimizadeh, Mohammad Pazhang Tags: RESEARCH ARTICLE Source Type: research

Maximizing Antibody Production in a Targeted Integration Host by Optimization of Subunit Gene Dosage and Position
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 21, 2020 Category: Biotechnology Authors: Joe Carver, Domingos Ng, Michelle Zhou, Peggy Ko, Dejin Zhan, Mandy Yim, David Shaw, Brad Snedecor, Michael W. Laird, Steven Lang, Amy Shen, Zhilan Hu Tags: RESEARCH ARTICLE Source Type: research

Holistic process development to mitigate proteolysis of a subunit rotavirus vaccine candidate produced in Pichia pastoris by means of an acid pH pulse during fed ‐batch fermentation
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 20, 2020 Category: Biotechnology Authors: M. Lourdes Velez ‐Suberbie, Stephen A. Morris, Kawaljit Kaur, John M. Hickey, Sangeeta B. Joshi, David B. Volkin, Daniel G. Bracewell, Tarit K. Mukhopadhyay Tags: RESEARCH ARTICLE Source Type: research

Effective temperature shift strategy development and scale confirmation for simultaneous optimization of protein productivity and quality in Chinese hamster ovary cells
AbstractTemperature shifts to lower culture temperatures are frequently employed in the manufacturing of protein therapeutics in mammalian cells to improve productivity, viability, or quality attributes. The direction and extent to which a temperature shift affects productivity and quality may vary depending on the expression host and characteristics of the expressed protein. We demonstrated here that two Chinese hamster ovary (CHO) clones expressing different human monoclonal antibodies responded differently to a temperature shift despite sharing a common parental CHO cell line. Within a single CHO line, we observed a non...
Source: Biotechnology Progress - January 17, 2020 Category: Biotechnology Authors: Kyle P. McHugh, Jianlin Xu, Kathryn L. Aron, Michael C. Borys, Zheng Jian Li Tags: RESEARCH ARTICLE Source Type: research

Biochemical characterization and structural insights into the high substrate affinity of a dimeric and Ca2+ independent Bacillus subtilis α‐amylase
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 17, 2020 Category: Biotechnology Authors: Karima Salem, Fatma Elgharbi, Hajer Ben Hlima, Massimiliano Perduca, Adel Sayari, A ïda Hmida‐Sayari Tags: RESEARCH ARTICLE Source Type: research

Response ‐Surface Methodology for the production and the purification of a new H2O2‐tolerant alkaline protease from Bacillus invictae AH1 strain
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 17, 2020 Category: Biotechnology Authors: Amal Hammami, Ahmed Bayoudh, Bilel Hadrich, Ola Abdelhedi, Mourad Jridi, Moncef Nasri Tags: RESEARCH ARTICLE Source Type: research

Biochemical characterization and structural insights into the high substrate affinity of a dimeric and Ca2+ independent Bacillus subtilis α‐amylase
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 17, 2020 Category: Biotechnology Authors: Karima Salem, Fatma Elgharbi, Hajer Ben Hlima, Massimiliano Perduca, Adel Sayari, A ïda Hmida‐Sayari Tags: RESEARCH ARTICLE Source Type: research

Response ‐Surface Methodology for the production and the purification of a new H2O2‐tolerant alkaline protease from Bacillus invictae AH1 strain
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 17, 2020 Category: Biotechnology Authors: Amal Hammami, Ahmed Bayoudh, Bilel Hadrich, Ola Abdelhedi, Mourad Jridi, Moncef Nasri Tags: RESEARCH ARTICLE Source Type: research

Development of Small ‐Scale Models to Understand the Impact of Continuous Downstream Bioprocessing on Integrated Virus Filtration
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 16, 2020 Category: Biotechnology Authors: Scott Lute, Julie Kozaili, Sarah Johnson, Kazuya Kobayashi, Daniel Strauss Tags: RESEARCH ARTICLE Source Type: research

Superhydrophobic Nanocarbon ‐based membrane with anti‐bacterial characteristics
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 14, 2020 Category: Biotechnology Authors: Mustafa Mohammed Aljumaily, Mohammed Abdulhakim Alsaadi, N. Awanis Hashim, Farouq S. Mjalli, Qusay F Alsalhy, Abdul Latif Khan, Ahmed Al ‐Harrasi Tags: RESEARCH ARTICLE Source Type: research

Generation of keratinocyte stem ‐like cells from human fibroblasts via a direct reprogramming approach
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 12, 2020 Category: Biotechnology Authors: Andong Zhao, Yi Yang, Xiaohua Pan, Yu Pan, Sa Cai Tags: RESEARCH ARTICLE Source Type: research

Effective temperature shift strategy development and scale confirmation for simultaneous optimization of protein productivity and quality in Chinese hamster ovary cells
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 12, 2020 Category: Biotechnology Authors: Kyle P. McHugh, Jianlin Xu, Kathryn L. Aron, Michael C. Borys, Zheng Jian Li Tags: RESEARCH ARTICLE Source Type: research

Use of Next ‐Generation Polymerized Human Hemoglobins in Hepatic Bioreactor Simulations
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 10, 2020 Category: Biotechnology Authors: Clayton Cuddington, Savannah Moses, Donald Belcher, Niral Ramesh, Andre Palmer Tags: RESEARCH ARTICLE Source Type: research

Optimized immobilization of endoglucanase Cel9A onto glutaraldehyde activated chitosan nanoparticles by Response Surface Methodology: The study of kinetic behaviors
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 10, 2020 Category: Biotechnology Authors: Saeed Najavand, Mahdiyeh Habibnejad, Ali Reza Amani ‐Ghadim, Parastou Rahimizadeh, Mohammad Pazhang Tags: RESEARCH ARTICLE Source Type: research

Use of Next ‐Generation Polymerized Human Hemoglobins in Hepatic Bioreactor Simulations
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 10, 2020 Category: Biotechnology Authors: Clayton Cuddington, Savannah Moses, Donald Belcher, Niral Ramesh, Andre Palmer Tags: RESEARCH ARTICLE Source Type: research

Atomistic probing of aptameric binding of CD19 outer membrane domain reveals an “aptamer walking” mechanism
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 8, 2020 Category: Biotechnology Authors: Michael K. Danquah, Hao ‐Bo Guo, Kei Xian Tan, Manoo Bhakta Tags: RESEARCH ARTICLE Source Type: research