Metabolic Trends of Chinese Hamster Ovary Cells in Biopharmaceutical Production under Batch and Fed ‐Batch Conditions
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - October 22, 2021 Category: Biotechnology Authors: Adam J. Rish, James K. Drennen, Carl A. Anderson Tags: REVIEW Source Type: research

Issue Information
(Source: Biotechnology Progress)
Source: Biotechnology Progress - October 18, 2021 Category: Biotechnology Tags: ISSUE INFORMATION Source Type: research

Efficient enzymatic synthesis of L ‐ascorbyl palmitate using Candida antarctica lipase B‐embedded metal‐organic framework
AbstractTheCandida antarctica lipase B (CALB) was embedded in the metal-organic framework, zeolitic imidazolate framework-8 (ZIF-8), and applied in the enzymatic synthesis ofL-ascorbic acid palmitate (ASP) for the first time. The obtained CALB@ZIF-8 achieved the enzyme loading of 80  mg g−1 with 11.3  U g−1 (dry weight) unit activity, 59.8% activity recovery, and 92.7% immobilization yield. Under the optimal condition, ASP was synthesized with over 75.9% conversion ofL-ascorbic acid in a 10-batch reaction. Continuous synthesis of ASP was subsequently performed in a packed bed bioreacto...
Source: Biotechnology Progress - October 18, 2021 Category: Biotechnology Authors: Xiao ‐Jian Zhang, Feng‐Yu Qi, Jia‐Mei Qi, Fei Yang, Jiang‐Wei Shen, Xue Cai, Zhi‐Qiang Liu, Yu‐Guo Zheng Tags: RESEARCH ARTICLE Source Type: research

Biophysical properties of tense quaternary state polymerized human hemoglobins bracketed between 500 kDa and 0.2 μm in size
AbstractPolymerized hemoglobin (Hb)-based oxygen carriers (HBOCs) are a scalable and cost-effective red blood cell (RBC) substitute. However, previous generations of commercial polymerized HBOCs elicited oxidative tissue injuryin vivo due to the presence of low molecular weight polymeric Hb species (
Source: Biotechnology Progress - October 9, 2021 Category: Biotechnology Authors: Clayton T. Cuddington, Savannah R. Wolfe, Andre F. Palmer Tags: RESEARCH ARTICLE Source Type: research

Characterization of cell cycle and apoptosis in Chinese hamster ovary cell culture using flow cytometry for bioprocess monitoring
AbstractChinese hamster ovary (CHO) cells are by far the most important mammalian cell lines used for producing antibodies and other therapeutic proteins. It is critical to fully understand their physiological conditions during a bioprocess in order to achieve the highest productivity and the desired product quality. Flow cytometry technology possesses unique advantages for measuring multiple cellular attributes for a given cell and examining changes in cell culture heterogeneity over time that can be used as metrics for enhanced process understanding and control strategy. Flow cytometry-based assays were utilized to exami...
Source: Biotechnology Progress - October 6, 2021 Category: Biotechnology Authors: Xiaodan Ji, Young Je Lee, Tom Eyster, Alexis Parrillo, Sybille Galosy, Zhaohui Ao, Pramthesh Patel, Yuan Zhu Tags: RESEARCH ARTICLE Source Type: research

X ‐ray sterilization of biopharmaceutical manufacturing equipment—Extractables profile of a film material and copolyester Tritan™ compared to gamma irradiation
AbstractThe biopharmaceutical industry gains enormous flexibility in production processes by using sterilized preassembled single-use devices. Gamma irradiation is an established sterilization technology that may be restricted in the future by the availability of60Co as irradiation source and irradiation capacities. X-ray technology is considered an alternative type of radiation for sterilizing SU equipment. In the context of extractables and leachables —one concern connected with the use of single-use process equipment—the effect of X-ray irradiation on the extractables profile of the materials needs to be com...
Source: Biotechnology Progress - October 4, 2021 Category: Biotechnology Authors: Roberto Menzel, Samuel Dorey, Tanja Maier, Ina Pahl, Armin Hauk Tags: RESEARCH ARTICLE Source Type: research

Efficient enzymatic synthesis of L ‐ascorbyl palmitate using CALB‐embedded metal‐organic framework
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - October 3, 2021 Category: Biotechnology Authors: Xiao ‐Jian Zhang, Feng‐Yu Qi, Jia‐Mei Qi, Fei Yang, Jiang‐Wei Shen, Xue Cai, Zhi‐Qiang Liu, Yu‐Guo Zheng Tags: RESEARCH ARTICLE Source Type: research

Assessment of Shock Pretreatment and Alkali Pretreatment on Corn Stover Using Enzymatic Hydrolysis
This study investigates digestibility enhancements of lignocellulose from shock pretreatment, alkaline pretreatment, and combination. Shock pretreatment subjects aqueous slurries of lignocellulose to shock waves, which disrupts its structure rendering it more susceptible to hydrolysis. Alkaline pretreatment submerges the biomass in aqueous alkali (NaOH, Ca(OH)2), which removes lignin and acetyl groups. As indicators of digestibility, cellulase (CTec3) and hemicellulase (HTec3) were used to saccharify the pretreated corn stover and the resulting filtrate which contains about 10% of the sugars. Shock is most effective when i...
Source: Biotechnology Progress - October 1, 2021 Category: Biotechnology Authors: Opeyemi Olokede, Shen ‐chun Hsu, Simon Schiele, Huang Ju, Mark Holtzapple Tags: RESEARCH ARTICLE Source Type: research

Rheology of Enzyme Liquefied Corn Stover Slurries: The Effect of Solids Concentration on Yielding and Flow Behavior
This study compared static yield stress values and flow hysteresis of corn stover slurries of 100, 150, 200, 250, and 300 g/L, after these slurries were formed by adding pellets to a cellulase enzyme solution (Celluclast 1.5L) in a fed-batch manner. A rotational rheometer was used to quantitate relative yield stress and its dependence on processing history at insoluble solids concentrations of 4 to 21% (w/v). Key findings confirmed previous observations that yield stress increases with solids loadings and reaches ~3,000 Pa at 25% (w/v) solids concentration compared to ~200 Pa after enzyme liquefaction. While optimization o...
Source: Biotechnology Progress - September 30, 2021 Category: Biotechnology Authors: Ryan Szeto, Jonathan C. Overton, Antonio Freitas dos Santos, Clark Eby, Nathan S. Mosier, Eduardo Ximenes, Michael R. Ladisch, Kendra A. Erk Tags: RESEARCH ARTICLE Source Type: research

Retrospective Assessment of Clonality of a Legacy Cell Line by Analytical Subcloning of the Master Cell Bank
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - September 29, 2021 Category: Biotechnology Authors: Guanghua Benson Li, Jennifer Pollard, Ren Liu, Richard C. Stevens, Jorge Quiroz, Michael C. Nelson, Matthew Manahan, Nicholas Murgolo, Robin S. Ehrick, Eric J. Wallenstein, Jason Hughes, Yung ‐shyeng Tsao, Jia Zhao, Zhimei Du, Nihal Tugcu, Tags: RESEARCH ARTICLE Source Type: research

Characterization of cell cycle and apoptosis in CHO cell culture using flow cytometry for bioprocess monitoring
AbstractChinese Hamster Ovary cells are by far the most important mammalian cell lines used for producing antibodies and other therapeutic proteins. It is critical to fully understand their physiological conditions during a bioprocess in order to achieve the highest productivity and the desired product quality. Flow cytometry technology possesses unique advantages for measuring multiple cellular attributes for a given cell and examining changes in cell culture heterogeneity over time that can be used as metrics for enhanced process understanding and control strategy. Flow cytometry-based assays were utilized to examine the...
Source: Biotechnology Progress - September 22, 2021 Category: Biotechnology Authors: Xiaodan Ji, Young Je Lee, Tom Eyster, Alexis Parrillo, Sybille Galosy, Zhaohui Ao, Pramthesh Patel, Yuan Zhu Tags: RESEARCH ARTICLE Source Type: research

Rapid intensification of an established CHO cell fed ‐batch process
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - September 20, 2021 Category: Biotechnology Authors: Markus Schulze, Julia Niemann, Rene H. Wijffels, Jens Matuszczyk, Dirk Martens Tags: RESEARCH ARTICLE Source Type: research

X ‐ray Sterilization of Biopharmaceutical Manufacturing Equipment ‐ Extractables Profile of a Film Material and Copolyester Tritan™ Compared to Gamma Irradiation
AbstractThe biopharmaceutical industry gains enormous flexibility in production processes by using sterilized pre-assembled single-use devices. Gamma irradiation is an established sterilization technology that may be restricted in the future by the availability of60Co as irradiation source and irradiation capacities. X-ray technology is considered an alternative type of radiation for sterilizing SU equipment. In the context of extractables and leachables – one concern connected with the use of single-use process equipment - the effect of X-ray irradiation on the extractables profile of the materials needs to be compa...
Source: Biotechnology Progress - September 20, 2021 Category: Biotechnology Authors: Roberto Menzel, Samuel Dorey, Tanja Maier, Ina Pahl, Armin Hauk Tags: RESEARCH ARTICLE Source Type: research

A Ribonucleoprotein ‐based Decaplex CRISPR/Cas9 Knockout Strategy for CHO Host Engineering
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - September 19, 2021 Category: Biotechnology Authors: Joseph Carver, Marie Kern, Peggy Ko, Midori Greenwood ‐Goodwin, X. Christopher Yu, Dana Duan, Danming Tang, Shahram Misaghi, Simon Auslaender, Ben Haley, Inn H. Yuk, Amy Shen Tags: RESEARCH ARTICLE Source Type: research

Application of a simple unstructured kinetic and cost of goods models to support T ‐cell therapy manufacture
AbstractManufacturing of cell therapy products requires sufficient understanding of the cell culture variables and associated mechanisms for adequate control and risk analysis. The aim of this study was to apply an unstructured ordinary differential equation-based model for prediction of T-cell bioprocess outcomes as a function of process input parameters. A series of models were developed to represent the growth of T-cells as a function of time, culture volumes, cell densities, and glucose concentration using data from the Ambr ®15 stirred bioreactor system. The models were sufficiently representative of the process t...
Source: Biotechnology Progress - September 15, 2021 Category: Biotechnology Authors: Maryam Shariatzadeh, Adriana G. Lopes, Katie E. Glen, Andrew Sinclair, Rob J. Thomas Tags: RESEARCH ARTICLE Source Type: research

Developments of biobased plasticizers for compostable polymers in the green packaging applications: A review
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - September 11, 2021 Category: Biotechnology Authors: Atika Alhanish, Mustafa Abu Ghalia Tags: REVIEW Source Type: research

Establishment and characterization of lung co ‐culture spheroids for paclitaxel loaded Eudragit® RL 100 nanoparticle evaluation
In this study, a simple setup was developed for consistent generation of lung co-culture spheroids, which were developed using the cancer cell lines A549, NCI H460, and fibroblast cells WI-38. The potential of these spheroids for evaluating the toxicity of Eudragit ® RL 100 nanoparticles (ENP) was explored. Monodisperse ENP, having the size range of 140–200 nm was prepared using the nanoprecipitation method. These were loaded with the poorly water-soluble anticancer drug paclitaxel. The evaluation of toxicity and uptake of drug-loaded ENP revealed that 2D monolayers were more sensitive to treatment than 3D...
Source: Biotechnology Progress - September 9, 2021 Category: Biotechnology Authors: Tejal Pant, Ganesh Gaikwad, Dhiraj Jain, Prajakta Dandekar, Ratnesh Jain Tags: RESEARCH ARTICLE Source Type: research

Erratum
(Source: Biotechnology Progress)
Source: Biotechnology Progress - September 8, 2021 Category: Biotechnology Tags: ERRATUM Source Type: research

Hybrid Fusion Protein as a Dual protease inhibitor for the Healing of Chronic Wounds
AbstractDiseases bring about the need for interventions that pinpoint each specific aspect of the illness. Commonly, remission of a complex disease is accomplished by mixing treatments, medications, and therapeutics together in a fashion where they may negatively interact with each other or never arrive at the diseased site as a systemic heterogeneous mixture. Chronic wounds display intricacy as they are very localized and have their own environment where tissue deconstruction due to high levels of numerous proteases outweighs normal tissue reconstruction. This idea leads to the necessity of a protein that contains low dif...
Source: Biotechnology Progress - September 6, 2021 Category: Biotechnology Authors: Graham Strauss, Piyush Koria Tags: RESEARCH ARTICLE Source Type: research

High ‐throughput and automation advances for accelerating single‐cell cloning, monoclonality and early phase clone screening steps in mammalian cell line development for biologics production
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - September 4, 2021 Category: Biotechnology Authors: Vijay Tejwani, Minal Chaudari, Toyaj Rai, Susan Sharfstein Tags: REVIEW Source Type: research

The Pandemic and Resilience for the Future: AccBio 2021
AbstractThe year 2020 brought the onslaught of a global crisis in the form of the COVID-19 pandemic. While nearly every facet of everyday life and work was impacted by the pandemic, the biopharmaceutical industry found silver linings in innovation, partnership, and resiliency, all of which contributed to unprecedented speed in developing and delivering vaccines and therapies. The 7th International Conference on Accelerating Biopharmaceutical Development (AccBio 2021) brought together industry leaders to share experiences from the past year and discuss how lessons learned from the pandemic can be carried forward into the fu...
Source: Biotechnology Progress - August 31, 2021 Category: Biotechnology Authors: Āine T. McGovern, Cleo M. Salisbury, Gregg B. Nyberg Tags: EDITORIAL Source Type: research

The effect of biosynthesized selenium nanoparticles on the expression of CYP51A and HSP90 antifungal resistance genes in Aspergillus fumigatus and A. flavus
This study takes a new approach to biosynthesized NPs influence on the expression ofCYP51A andHSP90 antifungal resistance genes inAspergillus fumigatus andA. flavus, and comparison with antifungal agents. Selenium NPs (Se-NPs) were biosynthesized usingAspergillus strains and their production was proved by several methods including, UV-Vis, XRD, FTIR, FESEM, and EDX techniques. The minimum inhibitory concentrations (MICs) ofAspergillus strains were determined using the CLSI M38-A2 broth microdilution method. The differences in expression levels ofCYP51A andHSP90 genes were examined between untreated and treated ofA. fumigat...
Source: Biotechnology Progress - August 30, 2021 Category: Biotechnology Authors: Mahdi Hosseini Bafghi, Razieh Nazari, Majid Darroudi, Mohsen Zargar, Hossein Zarrinfar Tags: APPLIED CELLULAR PHYSIOLOGY AND METABOLIC ENGINEERING Source Type: research

Evaluation of Single ‐use Tangential Flow Filtration Technology for Purification of Activated Polysaccharides Used in Conjugate Vaccine Manufacturing
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - August 30, 2021 Category: Biotechnology Authors: Seyed Pouria Motevalian, Jonathan Steen, Janine De Leon, Verl Sriskanda, Ivette Carino, Amar S. Prashad, Brenda Carrillo Conde, Bo Arve Tags: RESEARCH ARTICLE Source Type: research

Application of a Simple Unstructured Kinetic and Cost of Goods (CoGs) Models to Support T ‐Cell Therapy Manufacture
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - August 29, 2021 Category: Biotechnology Authors: Maryam Shariatzadeh, Adriana G. Lopes, Katie E. Glen, Andrew Sinclair, Rob J. Thomas Tags: RESEARCH ARTICLE Source Type: research

Antennapedia ‐derived positively‐charged peptide faces multiple problems upon their usage as targeting ligand for liposomal doxorubicin
AbstractThe positively-charged peptide antp derived from Antennapedia transcription protein is demonstrated to mediate the liposome translocation across the cell membrane. In the current investigation, we prepared a stable liposomal doxorubicin (Dox) formulation and targeted it with the antp peptide from 0 to 200 ligand/liposome. These antp-containing liposomes were investigated in terms of physical stability on storage in the refrigerator and upon incubation in blood. Also, other features like cell binding, uptake, biodistribution, and treatment efficiency were evaluated in C26 colon carcinoma BALB/c mice. The Antp in lip...
Source: Biotechnology Progress - August 28, 2021 Category: Biotechnology Authors: Sima Kousani, Maryam Karimi, Manouchehr Teymouri, Jamshid gholizadeh Navashenaq, Shahrzad Amiri Darban, Mahmoud Reza Jaafari Tags: RESEARCH ARTICLE Source Type: research

Application of a Simple Unstructured Kinetic and Cost of Goods (CoGs) Models to Support T ‐Cell Therapy Manufacture
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - August 28, 2021 Category: Biotechnology Authors: Maryam Shariatzadeh, Adriana G. Lopes, Katie E. Glen, Andrew Sinclair, Rob J. Thomas Tags: RESEARCH ARTICLE Source Type: research

Establishment and characterization of lung co ‐culture spheroids for Paclitaxel loaded Eudragit RL 100 nanoparticle evaluation
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - August 25, 2021 Category: Biotechnology Authors: Tejal Pant, Ganesh Gaikwad, Dhiraj Jain, Prajakta Dandekar, Ratnesh Jain Tags: RESEARCH ARTICLE Source Type: research

Metabolic engineering of Yarrowia lipolytica for the production of isoprene
AbstractYarrowia lipolytica has recently emerged as a prominent microbial host for production of terpenoids. Its robust metabolism and growth in wide range of substrates offer several advantages at industrial scale. In the present study, we investigate the metabolic potential ofY. lipolytica to produce isoprene. Sustainable production of isoprene has been attempted through engineering several microbial hosts; however, the engineering studies performed so far are challenged with low titers. Engineering ofY. lipolytica, which have inherent high acetyl-CoA flux could fuel precursors into the biosynthesis of isoprene and thus ...
Source: Biotechnology Progress - August 23, 2021 Category: Biotechnology Authors: Kurshedaktar M. Shaikh, Annamma A. Odaneth Tags: RESEARCH ARTICLE Source Type: research

Antennapedia ‐derived positively‐charged peptide faces multiple problems upon their usage as targeting ligand for liposomal doxorubicin
AbstractThe positively-charged peptide antp derived from Antennapedia transcription protein is demonstrated to mediate the liposome translocation across the cell membrane. In the current investigation, we prepared a stable liposomal doxorubicin (Dox) formulation and targeted it with the antp peptide from 0 to 200 ligand/liposome. These antp-containing liposomes were investigated in terms of physical stability on storage in the refrigerator and upon incubation in blood. Also, other features like cell binding, uptake, biodistribution, and treatment efficiency were evaluated in C26 colon carcinoma BALB/c mice. The Antp in lip...
Source: Biotechnology Progress - August 18, 2021 Category: Biotechnology Authors: Sima Kousani, Maryam Karimi, Manouchehr Teymouri, Jamshid Gholizadeh Navashenagh, Shahrzad Amiri Darban, Mahmoud Reza Jaafari Tags: RESEARCH ARTICLE Source Type: research

Differential responses in EPA and fucoxanthin production by the marine diatom Stauroneis sp. under varying cultivation conditions
AbstractThere has been an increasing drive toward better valorising raw biological materials in the context of the sustainability of bio-based industries and the circular economy. As such, microalgae hold the ability to biosynthesise valuable metabolites, which are sought after within the bioenergy, pharmaceuticals, cosmetics or nutrition sectors. Owing to their bioactivities, the xanthophyll pigment fucoxanthin and the omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA) have fostered increasing interests in terms of sustainably refining them from natural sources, such as microalgae. Together with the suitabilit...
Source: Biotechnology Progress - August 13, 2021 Category: Biotechnology Authors: Rachel Parkes, Lorraine Archer, D ónal Mc Gee, Thomas J. Smyth, Eoin Gillespie, Nicolas Touzet Tags: RESEARCH ARTICLE Source Type: research

Issue Information
(Source: Biotechnology Progress)
Source: Biotechnology Progress - August 12, 2021 Category: Biotechnology Tags: ISSUE INFORMATION Source Type: research

PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing
This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3N4/nettles/trachyspermum nanofiber by electrospinning method as a wound dressing. The g-C3N4 and g-C3N4 solution were synthesized and then were characterized using Fourier transform infrared, X-ray diffraction, Zeta Potential, and scanning electronic microscope analyzes. Also, the antibacterial properties of the synthesized materials were proved by gram-positive and gram-negative bacteria using the minimum inhibitory concentration method. Besides, the toxicity, migration, and ...
Source: Biotechnology Progress - August 12, 2021 Category: Biotechnology Authors: Danial Nemati, Mohsen Ashjari, Hamid Rashedi, Fatemeh Yazdian, Mona Navaei ‐Nigjeh Tags: RESEARCH ARTICLE Source Type: research

Microfluidic chip ‐based single‐cell cloning to accelerate biologic production timelines
AbstractCell line development (CLD) represents a critical, yet time-consuming, step in the biomanufacturing process as significant resources are devoted to the scale-up and screening of several hundreds to thousands of single-cell clones. Typically, transfected pools are fully recovered from selection and characterized for growth, productivity, and product quality to identify the best pools suitable for single-cell cloning (SCC) using limiting dilution or fluorescence-activated cell sorting (FACS). Here we report the application of the Berkeley Lights Beacon Instrument (BLI) in an early SCC process to accelerate the CLD ti...
Source: Biotechnology Progress - August 10, 2021 Category: Biotechnology Authors: Jonathan Diep, Huong Le, Kim Le, Ewelina Zasadzinska, Jasmine Tat, Pheng Yam, Ryan Zastrow, Natalia Gomez, Jennitte Stevens Tags: RESEARCH ARTICLE Source Type: research

Exometabolome profiling reveals activation of the carnitine buffering pathway in fed ‐batch cultures of CHO cells co‐fed with glucose and lactic acid
This study focused on the CHO cell's metabolic shift from the fifth day of culture. We compared relative levels of extracellular metabolites in the absence or presence of a 2  g/L lactic acid setpoint while glucose was kept at 4 g/L. Our hypothesis is that extra lactic acid would supply more pyruvate, favoring oxidative phosphorylation. We subsequentially uncovered several carnitine derivatives as biomarkers of the simultaneous activation of TCA anaplerotic pathways as well as a carbon-buffering pathway. CHO cells exhibited a balance between intermediates from (i) amino acid catabolism, (ii) fatty acidβ-oxid...
Source: Biotechnology Progress - August 10, 2021 Category: Biotechnology Authors: Johanna Vappiani, Tom Eyster, Keegan Orzechowski, Diana Ritz, Pramthesh Patel, Daniel S évin, Juan Aon Tags: RESEARCH ARTICLE Source Type: research

Metabolic engineering of Yarrowia lipolytica for the production of isoprene
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - August 9, 2021 Category: Biotechnology Authors: Kurshedaktar M. Shaikh, Annamma A. Odaneth Tags: RESEARCH ARTICLE Source Type: research

In silico process characterization for biopharmaceutical development following the quality by design concept
This study demonstrates the application of a previously published mechanistic chromatography model to the in silico process characterization (PCS) of a monoclonal antibody polishing step. The proposed modeling workflow covered the main tasks of traditional PCS studies following the QbD principles, including criticality assessment of 11 process parameters and establishment of their proven acceptable ranges of operation. Analyzing effects of multi-variate sampling of process parameters on the purification outcome allowed identification of the edge-of-failure. Experimental validation of in silico results demanded approximatel...
Source: Biotechnology Progress - August 5, 2021 Category: Biotechnology Authors: David Saleh, Gang Wang, Federico Rischawy, Simon Kluters, Joey Studts, J ürgen Hubbuch Tags: RESEARCH ARTICLE Source Type: research

PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing
This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3N4/nettles/trachyspermum nanofiber by electrospinning method as a wound dressing. The g-C3N4 and g-C3N4 solution were synthesized and then were characterized using FTIR, XRD, Zeta Potential, and SEM analyzes. Also, the antibacterial properties of the synthesized materials were proved by gram-positive and gram-negative bacteria using the MIC method. Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluate...
Source: Biotechnology Progress - August 4, 2021 Category: Biotechnology Authors: Danial Nemati, Mohsen Ashjari, Hamid Rashedi, Fatemeh Yazdian, Mona Navaei ‐Nigjeh Tags: RESEARCH ARTICLE Source Type: research