Real ‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning
AbstractReal-time, detailed online information on cell cultures is essential for understanding modern biopharmaceutical production processes. The determination of key parameters, such as cell density and viability, is usually based on the offline sampling of bioreactors. Gathering offline samples is invasive, has a low time resolution, and risks altering or contaminating the production process. In contrast, measuring process parameters online provides more safety for the process, has a high time resolution, and thus can aid in timely process control actions. We used online double differential digital holographic microscopy...
Source: Biotechnology Progress - December 14, 2022 Category: Biotechnology Authors: Jort J. Altenburg,
Maarten Klaverdijk,
Damien Cabosart,
Laurent Desmecht,
Sonja S. Brunekreeft ‐Terlouw,
Joshua Both,
Vivian I. P. Tegelbeckers,
Marieke L. P. M. Willekens,
Linda van Oosten,
Tessy A. H. Hick,
Tom M. H. van der Aalst,
Gorben P Tags: RESEARCH ARTICLE Source Type: research
Transmission of poly(dT60) single ‐stranded DNA through polycarbonate track‐etched ultrafiltration membranes
The objective of this study was to examine membrane filtration of a single stranded DNA (ssDNA) with 60 thymine nucleotides, and to elucidate the variables controlling its transmission across track-etched porous membranes. Dead end filtration measurements were performed using different pore size membranes (10, 15, and 30 nm) at different transmembrane pressures in solutions with ionic strength ranging from 0 to 1000 mM NaCl. The diffusivity of the ssDNA was determined using fluorescence recovery after photobleaching, yielding hydrodynamic radii ranging from 1.6 to 2.8 nm, with values decreasing with increasing soluti...
Source: Biotechnology Progress - December 12, 2022 Category: Biotechnology Authors: Hossein Nouri Alavijeh,
Ruth E. Baltus Tags: RESEARCH ARTICLE Source Type: research
Mechanistic modeling, simulation, and optimization of mixed ‐mode chromatography for an antibody polishing step
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - December 6, 2022 Category: Biotechnology Authors: Tobias Hahn,
Nora Geng,
Katerina Petrushevska ‐Seebach,
Michael E. Dolan,
Marcus Scheindel,
Pia Graf,
Kosuke Takenaka,
Kyo Izumida,
Lijuan Li,
Zijian Ma,
Norbert Schuelke Tags: RESEARCH ARTICLE Source Type: research
