Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”
(Source: Biotechnology Progress)
Source: Biotechnology Progress - January 30, 2024 Category: Biotechnology Tags: CORRECTION Source Type: research
Development of bioreactor scale ‐down model using orthogonal projections to latent structures method and CO2 supplementation
AbstractScale-down model qualification is an important step for developing a large-scale cell culture process to enhance process understanding and support process characterization studies. Traditionally, only harvest data are used to show consistency between small-scale and large-scale bioreactor performance, allowing attributes that are dynamic over the cell culture period to be overlooked. A novel statistical method, orthogonal projections to latent structures (OPLS) analysis, can be utilized to compare time-course cell culture data across scales. Here we describe an example where OPLS is used to identify gaps between sm...
Source: Biotechnology Progress - January 30, 2024 Category: Biotechnology Authors: Jinxin Gao,
Laurie B. Hazeltine,
Neal Stroud,
Ning Liu,
Yao ‐ming Huang Tags: RESEARCH ARTICLE Source Type: research
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”
(Source: Biotechnology Progress)
Source: Biotechnology Progress - January 30, 2024 Category: Biotechnology Tags: CORRECTION Source Type: research
A custom 3D printed paddlewheel improves growth in flat panel photobioreactor
AbstractOne of the main challenges with using flat panel photobioreactors for algal growth is uneven mixing and settling of cells in corners, especially when bubbling is the only method used for mixing. In order to improve mixing in our flat panel reactor, we designed a custom paddlewheel. Paddlewheels are frequently used in outdoor algae raceway ponds to improve mixing and we are taking advantage of the same principle for mixing in the reactor. The paddlewheel is easily integrated into our PSI FMT150 1-L flat panel photobioreactor and is printed on a 3D printer using high temperature poly lactic acid (HT-PLA). With the in...
Source: Biotechnology Progress - January 22, 2024 Category: Biotechnology Authors: Michelle Meagher,
Jacob Tamburro,
Nanette R. Boyle Tags: RESEARCH ARTICLE Source Type: research
Transitioning from static to suspension culture system for large ‐scale production of xeno‐free extracellular vesicles derived from mesenchymal stromal cells
In this study, we reported the establishment of a scalable bioprocess to produce MSC-EV in suspension cultures using spinner flasks and human collagen-coated microcarriers (3D culture system). We compared the EV production in this 3D culture system with the standard static culture using T-flasks (2D culture system). The EV produced in both systems were characterized and quantify by western blotting and nanoparticle tracking analysis. The presence of the typical protein markers CD9, CD63, and CD81 was confirmed by western blotting analyses for EV produced in both culture systems. The cell fold-increase was 5.7-fold for the ...
Source: Biotechnology Progress - January 22, 2024 Category: Biotechnology Authors: Nat ália Cristine Dias dos Santos,
Paula Bruzadelle‐Vieira,
Nádia de Cássia Noronha,
Amanda Mizukami‐Martins,
Maristela Delgado Orellana,
Maria Vitória L. B. Bentley,
Dimas Tadeu Covas,
Kamilla Swiech,
Kelen Cristina Ribeiro Malmegrim Tags: RESEARCH ARTICLE Source Type: research
Control strategy for biopharmaceutical production by model predictive control
AbstractThe biopharmaceutical industry is rapidly advancing, driven by the need for cutting-edge technologies to meet the growing demand for life-saving treatments. In this context, Model Predictive Control (MPC) has emerged as a promising solution to address the complexity of modern biopharmaceutical production processes. Its ability to optimize operations and ensure consistent product yields has made it an attractive option for manufacturers in this sector. Furthermore, MPC's alignment with the Process Analytical Technology (PAT) initiative provides an additional layer of assurance, facilitating real-time monitoring and ...
Source: Biotechnology Progress - January 11, 2024 Category: Biotechnology Authors: Touraj Eslami,
Alois Jungbauer Tags: REVIEW Source Type: research
Adaptive modeling optimized by the data fusion strategy: Real ‐time dying cell percentage prediction using capacitance spectroscopy
In conclusion, this study illustrates that the data fusion strategy substantially enhances the model accuracy compared to the previous model relying solely on capacitance spectra. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 5, 2024 Category: Biotechnology Authors: Suyang Wu,
Stephanie A. Ketcham,
Claudia Corredor,
Douglas Both,
Yuxiang Zhao,
James K. Drennen,
Carl A. Anderson Tags: RESEARCH ARTICLE Source Type: research
Chitosan based hybrid superabsorbent for controlled drug delivery application
AbstractIn the present study, a hybrid chitosan-alginate superabsorbent is prepared using maleic acid as a cross-linker and acrylamide as a grafting agent using the free radical mechanism. The composite hydrogel shows good swelling capacity along with hemocompatibility and biocompatibility and hence it is utilized as a drug delivery device. The characterization techniques including x-ray diffraction, Fourier transform infrared, x-ray photoelectron spectroscopy, and thermal analysis indicate the successful synthesis of stable hydrogel with rich functionalities. Metformin hydrochloride is used as a model drug which is used t...
Source: Biotechnology Progress - January 4, 2024 Category: Biotechnology Authors: Medha,
Sapna Sethi Tags: RESEARCH ARTICLE Source Type: research
Issue Information
(Source: Biotechnology Progress)
Source: Biotechnology Progress - January 3, 2024 Category: Biotechnology Tags: ISSUE INFORMATION Source Type: research
