Development of chondrocyte ‐laden alginate hydrogels with modulated microstructure and properties for cartilage regeneration
This study aims to investigate the applicability of sodium citrate buffer (SCB) as a culture medium supplement for modulating the microstructure of alginate microbeads to provide a favorable microenvironment for chondrogenic induction. The chondrocyte-laden microbeads, with and without TGF- β3 incorporation, were produced through an encapsulator. The obtained small-sized microbeads (~300 μm) were exposed to a treatment medium containing SCB, composed of varied concentrations of sodium citrate (1.10 – 1.57 mM), sodium chloride (3.00 – 4.29 mM), and ethylenediaminetetraacetic a cid (0.60 – 0.86 mM) to partially...
Source: Biotechnology Progress - December 24, 2022 Category: Biotechnology Authors: Tareerat Lertwimol,
Pacharapan Sonthithai,
Weerawan Hankamolsiri,
Pakkanun Kaewkong,
Paweena Uppanan Tags: RESEARCH ARTICLE Source Type: research
Accelerated CMC Workflows to enable Speed to Clinic in the COVID ‐19 era: A multi‐company view from the Biopharmaceutical Industry
This article provides an in-depth analysis of this data, ultimately highlighting an industry perspective of how biopharmaceutical companies can sustainably adopt new approaches to therapeutic protein development and production.This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - December 23, 2022 Category: Biotechnology Authors: Matthew F. Higgins,
Nicholas Abu ‐Absi,
Elena Gontarz,
Ingo H. Gorr,
Klaus Kaiser,
Pramthesh Patel,
Frank Ritacco,
Patrick Sheehy,
Bala Thangaraj Kumaar,
Tony Gill Tags: REVIEW Source Type: research
A Genetic Off ‐Target Event in a Site‐Specific Integration Cell Line Expressing Monoclonal Antibody has no Impact on Commercial Suitability
AbstractSite-specific integration (SSI) cell line systems are gaining popularity for biotherapeutic development and production. Despite the proven advantages for these expression hosts, the SSI system is still susceptible to rare off-target events and potential vector rearrangements. Here we describe the development process of an SSI cell line for production of an IgG1 monoclonal antibody (mAb-086). During cell line generational studies to assess suitability of clone C10 for commercial purposes, restriction fragment lengths of genomic DNA harboring the light chain (LC) were not in agreement with the predicted size. We firs...
Source: Biotechnology Progress - December 22, 2022 Category: Biotechnology Authors: Barbara Tevelev,
Andre Chambers,
Swap Ghosh,
Ying Zhang,
Lisa Marzili,
Jason C. Rouse,
Shu Han,
Mark Moffat,
John Scarcelli Tags: RESEARCH ARTICLE Source Type: research
Issue Information
(Source: Biotechnology Progress)
Source: Biotechnology Progress - December 20, 2022 Category: Biotechnology Tags: ISSUE INFORMATION Source Type: research
Dentin Regeneration based on Tooth Tissue Engineering: A review
This study investigates dentin regeneration after an injury such as caries using TE and stem/progenitor cell-based strategies. We begin by discussing about the biological structure of a dentin and dentinogenesis. The engineering of teeth requires knowledge of the processes that underlie the growth of an organ or tissue. Then, the three fundamental requirements for dentin regeneration, namely cell sources, GFs, and scaffolds are covered in the current study, which may ultimately lead to new insights in this field. (Source: Biotechnology Progress)
Source: Biotechnology Progress - December 17, 2022 Category: Biotechnology Authors: Ehsaneh Azaryan,
Fariba Emadian Razavi,
Mohammad Yahya Hanafi ‐Bojd,
Esmat Alemzadeh,
Mohsen Naseri Tags: REVIEW Source Type: research
A low ‐cost, high‐throughput microfluidic nano‐culture platform for functional metagenomics
AbstractFunctional metagenomics is an attractive culture-independent approach for functional screening of diverse microbiomes to identify known and novel genes. Since functional screening can involve sifting through tens of thousands of metagenomic library clones, an easy high-throughput screening approach is desirable. Here, we demonstrate a proof-of-concept application of a low-cost, high-throughput droplet based microfluidic assay to the selection of antibiotic resistance genes from a soil metagenomic library. Metagenomic library members encapsulated in nano-liter volume water-in-oil droplets were printed on glass slide...
Source: Biotechnology Progress - December 14, 2022 Category: Biotechnology Authors: Manpreet Kaur,
Aura Ferreiro,
Cheng ‐Yu Hung,
Gautam Dantas,
Anand K. Ramasubramanian Tags: NOTE Source Type: research
Real ‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning
AbstractReal-time, detailed online information on cell cultures is essential for understanding modern biopharmaceutical production processes. The determination of key parameters, such as cell density and viability, is usually based on the offline sampling of bioreactors. Gathering offline samples is invasive, has a low time resolution, and risks altering or contaminating the production process. In contrast, measuring process parameters online provides more safety for the process, has a high time resolution, and thus can aid in timely process control actions. We used online double differential digital holographic microscopy...
Source: Biotechnology Progress - December 14, 2022 Category: Biotechnology Authors: Jort J. Altenburg,
Maarten Klaverdijk,
Damien Cabosart,
Laurent Desmecht,
Sonja S. Brunekreeft ‐Terlouw,
Joshua Both,
Vivian I. P. Tegelbeckers,
Marieke L. P. M. Willekens,
Linda van Oosten,
Tessy A. H. Hick,
Tom M. H. van der Aalst,
Gorben P Tags: RESEARCH ARTICLE Source Type: research
Transmission of poly(dT60) single ‐stranded DNA through polycarbonate track‐etched ultrafiltration membranes
The objective of this study was to examine membrane filtration of a single stranded DNA (ssDNA) with 60 thymine nucleotides, and to elucidate the variables controlling its transmission across track-etched porous membranes. Dead end filtration measurements were performed using different pore size membranes (10, 15, and 30 nm) at different transmembrane pressures in solutions with ionic strength ranging from 0 to 1000 mM NaCl. The diffusivity of the ssDNA was determined using fluorescence recovery after photobleaching, yielding hydrodynamic radii ranging from 1.6 to 2.8 nm, with values decreasing with increasing soluti...
Source: Biotechnology Progress - December 12, 2022 Category: Biotechnology Authors: Hossein Nouri Alavijeh,
Ruth E. Baltus Tags: RESEARCH ARTICLE Source Type: research
