Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803
AbstractIn nature, photosynthetic organisms are exposed to fluctuating light, and their physiological systems must adapt to this fluctuation. To maintain homeostasis, these organisms have a light fluctuation photoprotective mechanism, which functions in both photosystems and metabolism. Although the photoprotective mechanisms functioning in the photosystem have been studied, it is unclear how metabolism responds to light fluctuations within a few seconds. In the present study, we investigated the metabolic response ofSynechocystis sp. PCC 6803 to light fluctuations using13C-metabolic flux analysis. The light intensity and ...
Source: Biotechnology Progress - February 9, 2023 Category: Biotechnology Authors: Tatsumi Imada,
Chiaki Yamamoto,
Masakazu Toyoshima,
Yoshihiro Toya,
Hiroshi Shimizu Tags: RESEARCH ARTICLE Source Type: research
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO/ZnO nanoparticles for superficial wound healing: in vitro and in vivo evaluations
In this study, Reduced Graphene Oxide (RGO)/ZnO NPs/Silk Fibroin nanocomposite was made, and an innovative nanofiber of SF/PVA/RGO/ZnO NPs was ready with the electrospinning technique and successfully characterized. The results of MIC and OD analyses were used to investigate the synthesized materials' antibacterial effects and displayed that the synthesized materials could inhibit growth againstS. aureus andE. coli bacteria. However, both in vitro cytotoxicity (MTT) and scratch wound studies have shown that RGO / ZnO NPs and SF/PVA/RGO/ZnO NPs are not only non-toxic to NIH 3T3 fibroblasts, but also can cause cell viability...
Source: Biotechnology Progress - February 8, 2023 Category: Biotechnology Authors: Maryam kavousi Heidari,
Mehrab Pourmadadi,
Fatemeh Yazdian,
Hamid Rashedi,
S. A. Seyyed Ebrahimi,
Zohreh Bagher,
Mona Navaei ‐Nigjeh,
Bibi Fatemeh Haghirosadat Tags: RESEARCH ARTICLE Source Type: research
Experimental and Computational Characterization of Mass Transfer in High Turndown Bioreactors
AbstractSingle-use bioreactors (SUBs, or disposable bioreactors) are extensively used for the clinical and commercial production of biologics. Despite widespread application, minimal results have been reported utilizing theturndown ratio; an operation mode where the working range of the bioreactor can be expanded to include low fluid volumes. In this work, a systematic investigation into free surface mass transfer and cell growth in high turndown single-use bioreactors is presented. This approach, which combines experimental mass transfer measurements with numerical simulation, deconvolutes the combined effects of headspac...
Source: Biotechnology Progress - February 8, 2023 Category: Biotechnology Authors: Evan M. Schlaich,
John A. Thomas,
Lakshmi Kandari,
Gabi Tremml,
Anurag Khetan Tags: RESEARCH ARTICLE Source Type: research
Integrated evaluation the antioxidant activity of epicatechin from cell dynamics
AbstractOxidative damage has been implicated in the pathogenesis of numerous disorders by affecting the normal functions of several tissues. Further, oxidative stress acts within cells to influence cell morphology and the behavior of cell migration. The movement and migration of cells are crucial during the development of organisms as they transition from embryo to adult, and for the homeostasis of adult tissues. Epicatechin (EC) is a natural flavonoid derived mostly from tea, chocolate, and red wine. We investigated the protective impact of EC on D-galactose(D-gal)/rotenone-injured NIH3T3 cells and found alterations in ce...
Source: Biotechnology Progress - February 4, 2023 Category: Biotechnology Authors: Yinglu Qing,
Xiong Xiang,
Shan Li,
Min Wang,
Zhengyang Liang,
Jiaoyan Ren Tags: RESEARCH ARTICLE Source Type: research
Advances in polymer ‐based cell encapsulation and its applications in tissue repair
AbstractCell microencapsulation is a more widely accepted area of biological encapsulation. In most cases, it involves fixing cells in polymer scaffolds or semi-permeable hydrogel capsules, providing the environment for protecting cells, allowing the exchange of nutrients and oxygen, and protecting cells against the attack of the host immune system by preventing the entry of antibodies and cytotoxic immune cells. Hydrogel encapsulation provides a three-dimensional (3D) environment similar to that experienced in vivo, so it can maintain normal cellular functions to produce tissues similar to those in vivo. Embedded cells ca...
Source: Biotechnology Progress - January 28, 2023 Category: Biotechnology Authors: Tangfang Lu,
Bin Xia,
Guobao Chen Tags: REVIEW Source Type: research
Integrated evaluation the antioxidant activity of epicatechin from cell dynamics
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 27, 2023 Category: Biotechnology Authors: Yinglu Qing,
Xiong Xiang,
Shan Li,
Min Wang,
Zhengyang Liang,
Jiaoyan Ren Tags: RESEARCH ARTICLE Source Type: research
Tuning Metabolic Efficiency for Increased Product Yield in High Titer Fed ‐Batch CHO Cell Culture
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 26, 2023 Category: Biotechnology Authors: Apolline Helfer,
Sylvain Gros,
Dave Kolwyck,
Daniel Karst Tags: RESEARCH ARTICLE Source Type: research
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 26, 2023 Category: Biotechnology Authors: Tatsumi Imada,
Chiaki Yamamoto,
Masakazu Toyoshima,
Yoshihiro Toya,
Hiroshi Shimizu Tags: RESEARCH ARTICLE Source Type: research
Single ‐stage chromatographic clarification of Chinese Hamster Ovary cell harvest reduces cost of protein production
AbstractA single-stage clarification was developed using a single-use chromatographic clarification device (CCD) to recover a recombinant protein from Chinese Hamster Ovary (CHO) harvest cell culture fluid (HCCF). Clarification of a CHO HCCF is a complex and costly process, involving multiple stages of centrifugation and/or depth filtration to remove cells and debris and to reduce process-related impurities such as host cell protein (HCP), nucleic acids, and lipids. When using depth filtration, the filter train consists of multiple filters of varying ratios, layers, pore sizes, and adsorptive properties. The depth filters,...
Source: Biotechnology Progress - January 25, 2023 Category: Biotechnology Authors: Brian O'Mara,
Naveen Kumar Singh,
Alicia Menendez,
Barbara Tipton,
Andrew Vail,
Alexei Voloshin,
Ying Buechler,
Sean M. Anderson Tags: RESEARCH ARTICLE Source Type: research
Advances in Polymer ‐based Cell Encapsulation and Its Applications in Tissue Repair
This article is protected by copyright. All rights reserved. (Source: Biotechnology Progress)
Source: Biotechnology Progress - January 19, 2023 Category: Biotechnology Authors: Tangfang Lu,
Bin Xia,
Guobao Chen Tags: REVIEW Source Type: research
