Issue Information
(Source: Biotechnology Progress)
Source: Biotechnology Progress - October 24, 2023 Category: Biotechnology Tags: ISSUE INFORMATION Source Type: research
Economic simulation of batch and continuous aqueous two ‐phase purification for viral products
AbstractVaccine manufacturing strategies that lower capital and production costs could improve vaccine access by reducing the cost per dose and encouraging localized manufacturing. Continuous processing is increasingly utilized to drive lower costs in biological manufacturing by requiring fewer capital and operating resources. Aqueous two-phase systems (ATPS) are a liquid –liquid extraction technique that enables continuous processing for viral vectors. To date, no economic comparison between viral vector purifications using traditional methods and ATPS has been published. In this work, economic simulations of traditiona...
Source: Biotechnology Progress - October 17, 2023 Category: Biotechnology Authors: Natalie M. Nold,
Eric Pearson,
Caryn L. Heldt Tags: RESEARCH ARTICLE Source Type: research
Shear stress and microRNAs for better metastatic cancer management
AbstractMetastasis is the process by which cancer cells move from the primary location to establish themselves in a new location in the human body. It is still a significant challenge in cancer management because it is responsible for 90% of cancer-related deaths. In this work, we present an idea to use shear stress encountered by all metastasizing cells as an elegant means to deactivate metastasizing cancer cells. Shear-induced ROS and cross-talk between ROS and miRNA play crucial roles in deactivating metastasizing cancer cells. In addition, there exists a vast therapeutic potential for miRNAs. Therefore, this study expl...
Source: Biotechnology Progress - October 16, 2023 Category: Biotechnology Authors: Siluveru KrishnaPriya,
Pallavi S. Nair,
Prerna Bhalla,
D. Karunagaran,
G. K. Suraishkumar Tags: RESEARCH ARTICLE Source Type: research
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support
AbstractIn this article, we have analyzed the interactions between enzyme crowding on a given support and its chemical modification (ethylenediamine modification via the carbodiimide route and picryl sulfonic (TNBS) modification of the primary amino groups) on the enzyme activity and stability. Lipase fromThermomyces lanuginosus (TLL) and lipase B fromCandida antarctica (CALB) were immobilized on octyl-agarose beads at two very different enzyme loadings, one of them exceeding the capacity of the support, one well under this capacity. Chemical modifications of the highly loaded and lowly loaded biocatalysts gave very differ...
Source: Biotechnology Progress - October 13, 2023 Category: Biotechnology Authors: Pedro Abellanas ‐Perez,
Diego Carballares,
Javier Rocha‐Martin,
Roberto Fernandez‐Lafuente Tags: RESEARCH ARTICLE Source Type: research
A novel method for continuous chromatographic separation of monoclonal antibody charge variants by combining displacement mode chromatography and step elution
In this study, we propose a novel approach for separation of charge variants during continuous CEX chromatography by utilizing a combination of displacement mode chromatography and salt-based step elution. It has been demonstrated that while the displacement mode of chromatography enables control of acidic variants ≤26% in the CEX eluate, salt-based step gradient elution manages basic charge variant ≤25% in the CEX eluate. The proposed approach has been successfully demonstrated using feed materials with varying compositions. On comparing the designed strategy with 2-column concurrent (CC) chromatography, the resin spe...
Source: Biotechnology Progress - October 13, 2023 Category: Biotechnology Authors: Anupa Anupa,
Vikrant Bansode,
Nikhil Kateja,
Anurag S. Rathore Tags: RESEARCH ARTICLE Source Type: research
Reusing wastewater from Coffea arabica processing to produce single ‐cell protein using Candida sorboxylosa: Optimizing of culture conditions
AbstractCoffee is a crop of significant socioeconomic importance, and the reuse of agri-food by-products and biowaste has great potential across several industries. Coffee wastewater (CWW) is a valuable resource containing essential nutrients that can be utilized byCandida sorboxylosa for single-cell protein (SCP) production. This utilization contributes to mitigating the negative impacts of agro-industrial waste. The optimization of culture conditions using the design of experiments (DoE) technique is crucial in understanding the environmental factors influencing metabolite production. In our study, the DoE technique was ...
Source: Biotechnology Progress - October 9, 2023 Category: Biotechnology Authors: Omar Santiago Pillaca ‐Pullo,
André Moreni Lopes,
Waldir D. Estela‐Escalante Tags: RESEARCH ARTICLE Source Type: research
Intramodule pressure profiles and protein accumulation during tangential flow filtration
AbstractTangential flow filtration (TFF) through a 30 kDa nominal molecular weight cut-off (MWCO) ultrafiltration membrane is widely employed to concentrate purified monoclonal antibodies (mAbs) to levels required for their formulation into injectable biologics. While TFF has been used to remove casein from milk for cheese production for over 35 y ears, and in pharmaceutical manufacture of biotherapeutic proteins for 20 years, the rapid decline in filtration rate (i.e., flux) at high protein concentrations is a limitation that still needs to be addressed. This is particularly important for mAbs, many of which are 14...
Source: Biotechnology Progress - October 2, 2023 Category: Biotechnology Authors: Fernanda Cunha,
Jessica Zuponcic,
Francesco Rossi,
Grant Springer,
Eduardo Ximenes,
Norvin Bruns,
John F. Moomaw,
Brian D. Bowes,
Ken K. Qian,
Zhao Yu,
Dennis Yang,
Vincent J. Corvari,
Arezoo Ardekani,
Gintaras Reklaitis,
Michael Ladisch Tags: RESEARCH ARTICLE Source Type: research
One ‐step production of fully biotinylated and glycosylated human Fc gamma receptors
AbstractInitiating and regulating humoral immunity, Fc gamma receptors (Fc γRs) have been identified both as therapeutics and as drug targets, and thus production of biologically active FcγRs is highly demanded for biopharmaceutical development. Focusing on low-affinity FcγRs IIA (131H/R allotypes), IIB, and IIIA (176F/V), this study used human 293-F cells to achieve co rrect post-translational modifications (PTMs) including biotinylation,N-glycosylation, and disulfides. Approaches involving co-expression of Fc γR-AviTag andEscherichia coli biotin ligase BirA, endoplasmic reticulum retention, stable and transient trans...
Source: Biotechnology Progress - September 22, 2023 Category: Biotechnology Authors: Minhyo Kang,
Zening Wang,
Xin Ge Tags: RESEARCH ARTICLE Source Type: research
